CN103519786B - Miniature cerebral apoplexy pathology inducible system for free-motion animal - Google Patents
Miniature cerebral apoplexy pathology inducible system for free-motion animal Download PDFInfo
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- CN103519786B CN103519786B CN201310473522.9A CN201310473522A CN103519786B CN 103519786 B CN103519786 B CN 103519786B CN 201310473522 A CN201310473522 A CN 201310473522A CN 103519786 B CN103519786 B CN 103519786B
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Abstract
The invention provides a miniature cerebral apoplexy pathology inducible system for a free-motion animal. The miniature cerebral apoplexy pathology inducible system comprises a support, an imaging device and a base. The support comprises a first optic fiber bundle and a second optic fiber bundle, wherein the first optic fiber bundle is used for irradiating a specific position on the surface of the brain of the free-motion animal to induct pathological changes of the position, and a specific medicine is injected to the animal; the second optic fiber bundle is used for lighting the imaging range of the surface of the brain of the animal. The imaging device is arranged on one side of the support and used for capturing and generating images through lighting of the second optic fiber bundle when the first optic fiber bundle irradiates the specific position of the surface of the brain of the animal. The base is arranged on the other side of the support and used for fixing the support on the surface of the brain of the animal. The support, the imaging device and the base are concentric.
Description
Technical field
The present invention relates to field of biomedicine technology, particularly a kind of miniature apoplexy pathology inducible system for freely-movable animal and method.
Background technology
Apoplexy is a kind of disease affecting brain medium-sized artery system.When apoplexy occurs, brain medium-sized artery is blocked or break, and blood in time to the well-off oxygen of cerebral tissue and nutriment, cannot cause brain cell death then.Apoplexy in situation occurred frequently, is the one of the main reasons causing patient death or deformity always.
In the research of apoplexy, zoopery is significant, and induces apoplexy to be carry out zooperal prerequisite in animal body.At present, in zoopery apoplexy pathology induction can simulate human body morbidity time pathophysiological change situation.But overwhelming majority's research of present stage is all test based on the animal of having anaesthetized, the impact of anesthetis on experiment cannot be got rid of.A large amount of research reports claims, and the pathophysiological change of different anesthetis on apoplexy has different appreciable impacts.Such as, the pathological tissues of anesthetis to apoplexy has and slows down apoptosis, reduces lesion volume, avoids the protective effects such as complication.For another example, under anesthetis effect, nervous system also has remarkable change to the reaction that body stimulates.Therefore, need to propose a kind of apoplexy abductive approach for sobering animal, improve current research.
Photochemical method is a kind of representative Wicresoft, fast, accurately apoplexy inductive technology.Research shows, some photoactive substance chemical change can occur under the exciting of specific wavelength illumination, and change product can react thus artery-clogging with blood vessel wall further.The apoplexy of photochemical method induction, due to the position of illumination, scope, energy-controllable, compared to additive method, has accurate, stable advantage.Therefore photochemical method has a wide range of applications.But the photochemical method of present stage still cannot get rid of the impact of anesthetis on experiment.
In addition, in the research process of apoplexy pathology, the early stage monitoring of pathological process is significant to searching optimal treatment window, therefore most important to the early monitoring of pathological process in clinical or experimentation.And optical image technology is as a kind of important Brian Imaging means, there is high-spatial and temporal resolution characteristic, be widely used in the research of brain science.But the experiment condition of present stage does not allow to complete the induction of apoplexy and the omnidistance Real-Time Monitoring of pathological process substantially simultaneously.Therefore, apoplexy inducible system and optical imaging system are be combined with each other, the detection method proposed in a kind of apoplexy Induction Process is particularly important.
Summary of the invention
The object of the present invention is to provide a kind of miniature apoplexy pathology inducible system for freely-movable animal and method, to get rid of the impact that anesthetis is induced apoplexy pathology, realize the omnidistance Real-Time Monitoring of apoplexy induction and pathological process simultaneously.
For solving the problems of the technologies described above, the invention provides a kind of miniature apoplexy pathology inducible system for freely-movable animal, comprising:
Support, described support comprises one first fibre bundle, for irradiating brain surface's ad-hoc location of a freely-movable animal, induces the pathological change of this position, and wherein, described animal was injected specific medicine;
Second fibre bundle, for the illumination of the brain surface's areas imaging to described animal;
Imaging device, is arranged at the side of described support, for the illumination by described second fibre bundle, catches and generates image when described first fibre bundle irradiates brain surface's ad-hoc location of described animal; And
Base, is arranged at the opposite side of described support, for described support being fixed on the brain surface of described animal;
Described support, imaging device and base keep same axle center.
Further, described in the miniature apoplexy pathology inducible system of freely-movable animal, described support also comprises metal tube, and the light of described first fibre bundle conduction penetrates from described metal tube.
Further, described in the miniature apoplexy pathology inducible system of freely-movable animal, described metal tube has the function regulating described first fibre bundle to be radiated at the angle of the brain surface of described animal.
Further, described in the miniature apoplexy pathology inducible system of freely-movable animal, described support and described imaging device are fixed by one group of Spring screws.
Further, described in the miniature apoplexy pathology inducible system of freely-movable animal, described imaging device comprises:
Camera lens, for catching image when described second fibre bundle irradiates brain surface's ad-hoc location of described animal.
Further, described in the miniature apoplexy pathology inducible system of freely-movable animal, the shape of described base is circular; The shape of the bottom of described support is also circular.
Meanwhile, the present invention also provides a kind of miniature apoplexy pathology abductive approach for freely-movable animal, uses the described miniature apoplexy pathology inducible system for freely-movable animal, comprising:
Specific medicine is injected to animal;
Be injected brain surface's ad-hoc location of the animal of specific medicine described in first fibre bundle irradiates, induce the pathological change of this position;
Second fibre bundle throws light on to the areas imaging of the brain surface of described animal;
Imaging device, by the illumination of described second fibre bundle, catches and generates image when described first fibre bundle irradiates brain surface's ad-hoc location of the animal being injected specific medicine.
Further, described in the miniature apoplexy pathology abductive approach of freely-movable animal, described support also comprises metal tube;
Brain surface's ad-hoc location of the animal of specific medicine was injected described in first fibre bundle irradiates, induce in the step of the pathological change of this position, by regulating metal tube, make the light of described first fibre bundle conduction be radiated at brain surface's ad-hoc location of described animal, induce the pathological change of this position.
Further, described in the miniature apoplexy pathology abductive approach of freely-movable animal, described imaging device comprises camera lens;
Imaging device is by the illumination of described second fibre bundle, catch and generate in the step of picture when described first fibre bundle irradiates brain surface's ad-hoc location of the animal being injected specific medicine, catching image when described first fibre bundle irradiates brain surface's ad-hoc location of the animal being injected specific medicine by camera lens.
Miniature apoplexy pathology inducible system for freely-movable animal provided by the invention and method, there is following beneficial effect: by the first fibre bundle inserted in metal tube, the light of conduction is radiated at brain surface's ad-hoc location of animal, thus can direct light chemical method need specific wavelength light irradiate animal brain surface, carry out the induction of apoplexy.In addition, the angle of metal tube can adjust, thus makes the light of the first fibre bundle conduction correctly can irradiate the brain surface of animal, at ad-hoc location induction apoplexy.
Further, when use second fibre bundle conducting light irradiates animal brain surface imaging scope, imaging device can realize omnidistance realtime imaging by camera lens, and the light of the second fibre bundle conduction can match with imaging technique.
Accompanying drawing explanation
Fig. 1-2 is the structural representation of the miniature apoplexy pathology inducible system for freely-movable animal of the embodiment of the present invention;
Fig. 3 is the flow chart of the miniature apoplexy pathology abductive approach for freely-movable animal of the embodiment of the present invention.
Detailed description of the invention
The miniature apoplexy pathology inducible system for freely-movable animal proposed the present invention below in conjunction with the drawings and specific embodiments and method are described in further detail.According to the following describes and claims, advantages and features of the invention will be clearer.It should be noted that, accompanying drawing all adopts the form that simplifies very much and all uses non-ratio accurately, only in order to object that is convenient, the aid illustration embodiment of the present invention lucidly.
As illustrated in fig. 1 and 2, the invention provides a kind of miniature apoplexy pathology inducible system for freely-movable animal, be applied to brain surface or the body surface (the present embodiment specifically describes brain surface) of animal, for the artificial induction of animal apoplexy disease under freely-movable state, described system comprises:
Support 110, described support 110 comprises one first fibre bundle 111, for irradiating brain surface's ad-hoc location of an animal 220, induces the pathological change of this ad-hoc location, and wherein, described animal 220 was injected specific medicine;
Second fibre bundle 123, for the illumination of the brain surface's areas imaging to described animal 220;
Imaging device 120, is arranged at the side of described support 110, and the present embodiment is top, is fixed, decrease the vibrations of imaging device 120 by one group of Spring screws 121 and support 110.Described imaging device 120, for the illumination by described second fibre bundle 123, catches and generates image when described first fibre bundle 111 irradiates brain surface's ad-hoc location of described animal 220; And
Base 210, is arranged at the opposite side of described support 110, and the present embodiment is bottom, for described support 110 being fixed on the brain surface of described animal 220;
Described support 110, imaging device 120 and base 210 keep same axle center.
Furthermore, described support 110 also comprises metal tube 112, the light that described first fibre bundle 111 conducts penetrates from described metal tube 112, and meanwhile, described metal tube 112 has the function regulating described first fibre bundle 111 to be radiated at the angle of the brain surface of described animal 220.That is, can by regulating the angle of metal tube 112, change the angle that the first fibre bundle 111 is radiated at the brain surface of described animal 220, the first fibre bundle 111 can be made more accurately to be radiated at specific scope, medicine within the scope of induction specific shot and blood vessel wall react thus artery-clogging, the pathological state of induction apoplexy.Whole process does not have obvious pain reaction, meets zooperal requirement.
Furthermore, described imaging device 120 comprises:
Camera lens 122, described second fibre bundle 123 is arranged at the outer ring of described camera lens 122, and described camera lens 122 is for catching image when described second fibre bundle 123 irradiates brain surface's areas imaging of described animal 220;
The present invention is when use second fibre bundle 123 conducting light irradiates the specific areas imaging of the brain surface of described animal 220, imaging device 120 can realize omnidistance realtime imaging by camera lens 122, and the light that the second fibre bundle 123 conducts can match with imaging technique.
Further, in the present embodiment, the shape of described base 210 is circular; The shape of the bottom of described support 110 is also circular.Such support 110 and base 210 just can mate, with fixing described support 110.After being fixed as one with described base 210 bottom described support 110, namely the picture being irradiated the brain surface of animal 220 by described second fibre bundle 123 is caught by described imaging device 120 by described camera lens 122.Meanwhile, the first upper fibre bundle 111 being fixed on described support 110 can induce generation apoplexy pathological state.
The present invention's base 210 is fixed on the brain surface of animal 220, and other parts are fixed on base 210 by support 110, thus in animal 220 motor process, native system can keep relative static conditions with animal 220 brain surface.
Meanwhile, as shown in Figure 3, the present invention also provides a kind of miniature apoplexy pathology abductive approach for freely-movable animal, uses the described miniature apoplexy pathology inducible system for freely-movable animal, comprising:
S31: specific medicine is injected to animal;
Be injected brain surface's ad-hoc location of the animal of specific medicine described in S32: the first fibre bundle irradiates, induce the pathological change of this ad-hoc location;
Concrete, by regulating metal tube, thus make the light of described first fibre bundle conduction be radiated at brain surface's ad-hoc location of described animal.
The light of conduction, by the first fibre bundle inserted in metal tube, is radiated at the brain surface of animal by the present invention, thus can direct light chemical method need specific wavelength light irradiate animal brain surface, carry out the induction of apoplexy.In addition, the angle of metal tube can adjust, thus the irradiation animal brain surface that the light that the first fibre bundle is guided can be correct, at ad-hoc location induction apoplexy.
S33: the second fibre bundle throws light on to the areas imaging of the brain surface of described animal;
S34: imaging device, by the illumination of described second fibre bundle, catches and generates image when described first fibre bundle irradiates brain surface's ad-hoc location of the animal being injected specific medicine.
Concrete, catch image when described first fibre bundle irradiates the brain surface of the animal being injected specific medicine by camera lens.
When use second fibre bundle conducting light irradiates animal brain surface, imaging device can realize omnidistance realtime imaging by camera lens, and the light of the second fibre bundle conduction can match with imaging technique.
Foregoing description is only the description to present pre-ferred embodiments, any restriction not to the scope of the invention, and any change that the those of ordinary skill in field of the present invention does according to above-mentioned disclosure, modification, all belong to the protection domain of claims.
Claims (6)
1., for a miniature apoplexy pathology inducible system for freely-movable animal, it is characterized in that, comprising:
Support, described support comprises one first fibre bundle, for irradiating brain surface's ad-hoc location of an animal, induces the pathological change of this position, and wherein, described animal was injected specific medicine;
Second fibre bundle, for the illumination of the brain surface's areas imaging to described animal;
Imaging device, is arranged at the side of described support, for the illumination by described second fibre bundle, catches and generates image when described first fibre bundle irradiates brain surface's ad-hoc location of described animal; And
Base, is arranged at the opposite side of described support, for described support being fixed on the brain surface of described animal;
Described support, imaging device and base keep same axle center.
2. as claimed in claim 1 for the miniature apoplexy pathology inducible system of freely-movable animal, it is characterized in that, described support also comprises metal tube, and the light of described first fibre bundle conduction penetrates from described metal tube.
3. as claimed in claim 2 for the miniature apoplexy pathology inducible system of freely-movable animal, it is characterized in that, described metal tube has the function regulating the light of described first fibre bundle conduction to be radiated at the angle of the brain surface of described animal.
4., as claimed in claim 1 for the miniature apoplexy pathology inducible system of freely-movable animal, it is characterized in that, described support and described imaging device are fixed by one group of Spring screws.
5., as claimed in claim 1 for the miniature apoplexy pathology inducible system of freely-movable animal, it is characterized in that, described imaging device comprises:
Camera lens, for catching image when described second fibre bundle irradiates brain surface's ad-hoc location of described animal.
6. as claimed in claim 1 for the miniature apoplexy pathology inducible system of freely-movable animal, it is characterized in that, the shape of described base is circular; The shape of the bottom of described support is also circular.
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| CN105962893A (en) * | 2016-04-25 | 2016-09-28 | 上海交通大学 | Endogenous photo-imaging system used for moving animal |
| CN107692974A (en) * | 2017-10-25 | 2018-02-16 | 上海交通大学 | A kind of multi-parameter animal cortex blood flow monitoring device and method |
| CN109009549B (en) * | 2018-08-20 | 2024-06-28 | 中国人民解放军军事科学院军事医学研究院 | Device for recording animal drug addiction and related brain region nerve activity law |
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| CN102247218A (en) * | 2011-03-25 | 2011-11-23 | 杨碧波 | Animal cardiac failure induction device |
| CN102379740A (en) * | 2010-09-06 | 2012-03-21 | 南京大学 | Arcus aortae constriction surgery method for inducing myocardial hypertrophy of mice |
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| CN102379740A (en) * | 2010-09-06 | 2012-03-21 | 南京大学 | Arcus aortae constriction surgery method for inducing myocardial hypertrophy of mice |
| CN102247218A (en) * | 2011-03-25 | 2011-11-23 | 杨碧波 | Animal cardiac failure induction device |
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