CN103497921B - The method of the preparation method of methane-oxidizing bacteria bacterium liquid, Covering Material of Refuse Landfill and refuse landfill methane emission reduction - Google Patents
The method of the preparation method of methane-oxidizing bacteria bacterium liquid, Covering Material of Refuse Landfill and refuse landfill methane emission reduction Download PDFInfo
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- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W30/00—Technologies for solid waste management
- Y02W30/30—Landfill technologies aiming to mitigate methane emissions
Abstract
The invention provides a kind of preparation method of methane-oxidizing bacteria bacterium liquid, comprise the following steps: mineralized waste is mixed with percolate, cultivate under passing into the condition of the mixed gas of methane and oxygen, obtain methane-oxidizing bacteria bacterium liquid, the methane oxidation rate of described mineralized waste is more than 50%.The invention provides a kind of a kind of method of Covering Material of Refuse Landfill and refuse landfill methane emission reduction.The present invention originates using mineralized waste as methane-oxidizing bacteria strain, using percolate as culture medium mixed culture methane-oxidizing bacteria, culture system and landfill yard environment comparatively close, the methane-oxidizing bacteria bacterium liquid obtained more adapts to refuse landfill environment, and the methane-oxidizing bacteria bacterium liquid onset obtained is rapid, higher to the oxidation efficiency of methane in landfill gas, when covering 7 days, methane oxidation rate can reach more than 84%.
Description
Technical field
The invention belongs to environment-friendly materials technical field, particularly relate to a kind of method of preparation method of methane-oxidizing bacteria bacterium liquid, Covering Material of Refuse Landfill and refuse landfill methane emission reduction.
Background technology
Refuse landfill stacks place, because cost is low, sanitation degree is well widely used at home in the refuse collecting under employing sanitary landfill mode.For aged and middle-size and small-size household refuse landfill sites, because methane concentration in the landfill gas of generation is low or generation is few, be difficult to recycle, therefore the general method arranging covering layer of landfill site that adopts carries out bio-oxidation to methane, thus controls and reduce methane release.There are some researches show that compost has good methane oxidation effect, can be used to the biological tectum preparing landfill yard, to improve tectal methane oxidation ability.But concerning China's major part household refuse landfill sites, the acquisition of compost is very not convenient, needs to find the covering material be more effectively easy to get.
In covering layer of landfill site, the quantity of methane-oxidizing bacteria is with active to methane oxidation process important, is therefore the key factor determining Methane Emissions.It is increase the most direct method of methane-oxidizing bacteria quantity in tectum that cultivation preparation methane-oxidizing bacteria bacterium liquid is applied in covering layer of landfill site, be conducive to provide the suitable condition of methane-oxidizing bacteria activity simultaneously, effectively can improve tectal methane oxidation ability, methane oxidation is started fast.
For obtaining high-efficiency methane oxidizing microorganisms system in existing research and engineer applied, the methane-oxidizing bacteria strain of cultivation carrys out the environment that own methane gas produces mostly, as the bed mud in water paddy soil, oil field surface soil, ocean and lake or settling etc.For the cultural method of methane-oxidizing bacteria, the screening of traditional environment engineering bacteria and culturing process more complicated, comprise the bacterial classification needed for selecting special substratum and being separated, then by series of steps such as Tube propagation, triangular flask cultivation, first order seed cultivation, secondary seed cultivation and fermentor cultivation progressively spread cultivation.But this method not only process is complicated, and the bacterium liquid obtained not necessarily can adapt to the complex environment of landfill yard.
Containing methane-oxidizing bacteria in mineralized waste, and bacterial classification is in the environment of landfill yard landfill gas for a long time, comparatively strong to landfill gas adaptability, therefore can originate as the bacterial classification of methane-oxidizing bacteria.The Chinese patent literature being 200710040358.7 as application number discloses one way of life refuse landfill methane oxidation covering material, is made up of mineralized waste and mineralising mud, and during for covering layer of landfill site, methane oxidation assimilated efficiency can improve 20% ~ 30%.But the tectum onset that the method obtains is comparatively slow, and covering its methane oxidation efficiency after one month can reach 70% ~ 80%.
Summary of the invention
The object of the present invention is to provide a kind of method of preparation method of methane-oxidizing bacteria bacterium liquid, Covering Material of Refuse Landfill and refuse landfill methane emission reduction, covering material onset provided by the invention is rapid, within a short period of time can reach higher methane conversion, and cost is lower.
The invention provides a kind of preparation method of methane-oxidizing bacteria bacterium liquid, comprise the following steps:
Mixed with percolate by mineralized waste, cultivate under passing into the condition of the mixed gas of methane and oxygen, obtain methane-oxidizing bacteria bacterium liquid, the methane oxidation rate of described mineralized waste is more than 50%.
Preferably, described mineralized waste carries out pre-treatment in accordance with the following methods:
The mixed gas passing into methane and oxygen in mineralized waste raw material is tamed, and tame and obtain mineralized waste to methane oxidation rate more than 50%, the temperature of described domestication is 28 DEG C ~ 37 DEG C.
Preferably, described mineralized waste raw material is the mineralized waste at 8 ages more than, and the particle diameter of described mineralized waste raw material is 0.5mm ~ 4cm, and the water ratio of described mineralized waste raw material is 20% ~ 30%.
Preferably, described percolate is percolate from garbage filling field, landfill percolate recharges process tail water or landfill percolate mineralising bed process tail water.
Preferably, described percolate is 8 age above percolates, in described percolate, total nitrogen content lower than 1400mg/L, Ni content lower than 0.4mg/L.
Preferably, the mass volume ratio of described mineralized waste and described percolate is 0.5g ~ 2g:100mL.
Preferably, the pH value of the mixed solution obtained after described mineralized waste and percolate mix is 7 ~ 9, and the temperature of described cultivation is 25 DEG C ~ 37 DEG C, is cultured to the cell concn OD of bacterium liquid
600more than 0.6.
Preferably, the volume ratio of described methane and oxygen is 1:1 ~ 1.3.
The invention provides a kind of Covering Material of Refuse Landfill, comprising: the methane-oxidizing bacteria bacterium liquid that mineralized waste and the method described in technique scheme prepare, described methane-oxidizing bacteria bacterium liquid accounts for the 8wt% ~ 12wt% of described mineralized waste.
Present invention also offers a kind of method of refuse landfill methane emission reduction, comprise the following steps:
The rubbish of landfill arranges tectum, and described tectum comprises the Covering Material of Refuse Landfill described in technique scheme.
Compared with prior art, the invention provides a kind of preparation method of methane-oxidizing bacteria bacterium liquid, comprise the following steps: mineralized waste is mixed with percolate, cultivate under passing into the condition of the mixed gas of methane and oxygen, obtain methane-oxidizing bacteria bacterium liquid, the methane oxidation rate of described mineralized waste is more than 50%.The present invention originates using mineralized waste as methane-oxidizing bacteria strain, using percolate as culture medium mixed culture methane-oxidizing bacteria, culture system and landfill yard environment comparatively close, the methane-oxidizing bacteria bacterium liquid obtained more adapts to refuse landfill environment, and the methane-oxidizing bacteria bacterium liquid onset obtained is rapid, higher to the oxidation efficiency of methane in landfill gas, when covering 7 days, methane oxidation rate can reach more than 84%.Simultaneously, percolate and mineralized waste are waste, respectively using it as culture medium and bacterial classification source, solve the problem of environmental pollution that percolate causes, achieve the recycling of mineralized waste, not only method is simple, effectively reduces the cost of refuse landfill methane gas process, and effectively expand the landfill capacity of refuse landfill, extend the working life of refuse landfill.In addition, cover in refuse landfill as covering material after methane-oxidizing bacteria bacterium liquid provided by the invention mixes with mineralized waste, effectively by the methane oxidation in landfill gas, thus the discharge of methane of landfill yard can be controlled, reach the object of the treatment of wastes with processes of wastes against one another.
Embodiment
The invention provides a kind of preparation method of methane-oxidizing bacteria bacterium liquid, comprise the following steps:
Mixed with percolate by mineralized waste, cultivate under passing into the condition of the mixed gas of methane and oxygen, obtain methane-oxidizing bacteria bacterium liquid, the methane oxidation rate of described mineralized waste is more than 50%.
The present invention originates using mineralized waste as methane-oxidizing bacteria strain, using percolate as culture medium mixed culture methane-oxidizing bacteria, culture system and landfill yard environment comparatively close, the methane-oxidizing bacteria bacterium liquid obtained more adapts to refuse landfill environment, and the methane-oxidizing bacteria bacterium liquid onset obtained is rapid, higher to the oxidation efficiency of methane in landfill gas, when covering 7 days, methane oxidation rate can reach more than 84%.
The present invention originates using mineralized waste as methane-oxidizing bacteria strain, and the methane oxidation rate of described mineralized waste is more than 50%.When the methane oxidation rate of mineralized waste is more than 50%, it can carry out using percolate as culture medium cultivating and obtain rapid-action, that efficiency is high methane-oxidizing bacteria bacterium liquid; When the methane oxidation rate of mineralized waste is less than 50%, it carries out cultivating the methane-oxidizing bacteria bacterium liquid obtained and does not have rapid-action, that efficiency is high feature using percolate as culture medium.
In the present invention, described mineralized waste is preferably through following pre-treatment:
The mixed gas passing into methane and oxygen in mineralized waste raw material is tamed, and tames to methane oxidation rate and obtains mineralized waste more than 50%.
In the present invention, described mineralized waste raw material is preferably the mineralized waste at 8 ages more than, is more preferably the mineralized waste at 10 ages more than; The particle diameter of described mineralized waste raw material is preferably 0.5mm ~ 4cm, is more preferably 0.8mm ~ 3.5cm; The water ratio of described mineralized waste raw material is preferably 20% ~ 30%, is more preferably 21% ~ 28%.
The mixed gas passing into methane and oxygen in described mineralized waste raw material is tamed, and tames to methane oxidation rate and can to obtain as methane-oxidizing bacteria strain mineralized waste more than 50%.In the present invention, the volume ratio of described methane and oxygen is preferably 1:1 ~ 1.3; When taming, described acclimation temperature is preferably 28 DEG C ~ 37 DEG C, is more preferably 30 DEG C ~ 35 DEG C.In domestication process, the methane-oxidizing bacteria in mineralized waste raw material obtains enrichment, makes the methane oxidation rate of mineralized waste reach more than 50%.
Obtain methane oxidation rate more than after the mineralized waste of 50%, cultivate it is mixed the condition of the mixed gas passing into methane and oxygen with percolate under, methane-oxidizing bacteria bacterium liquid can be obtained.In the present invention, the effect of described percolate is as culture medium, nutritive substance wherein provides the nutrition required for methane oxidizing bacteria growth and activity, and described percolate can recharge process tail water or landfill percolate mineralising bed process tail water for percolate from garbage filling field, landfill percolate.Specifically, described percolate is preferably the percolate at 8 ages more than, in described percolate, total nitrogen content preferably lower than 1400mg/L, more preferably less than 1200mg/L; Ni content preferably lower than 0.4g/L, more preferably less than 0.3g/L.The mass volume ratio of described mineralized waste and percolate is preferably 0.5g ~ 2g:100mL, is more preferably 0.8g ~ 1.8g:100mL.
After mineralized waste and percolate mixing, cultivate after obtaining mixed solution, the pH value of described mixed solution is preferably 7 ~ 9, is more preferably 7.3 ~ 8.8.The present invention take percolate as the methane-oxidizing bacteria that culture medium cultivates in mineralized waste, and obtain methane-oxidizing bacteria bacterium liquid, the temperature of described cultivation is preferably 25 DEG C ~ 37 DEG C, is more preferably 28 DEG C ~ 35 DEG C; The time of described cultivation is preferably 2 days ~ 3 days, is cultured to the cell concn OD of bacterium liquid
600more than 0.6, wherein, OD
600represent, cell concn represents with the photoabsorption under 600nm wavelength.Described cultivation is carried out under the condition of mixed gas passing into methane and oxygen, be specifically as follows: after mineralized waste and percolate mixing, after passing into the mixed gas of methane and oxygen, sealing is cultivated, and described cultivation can be cultivated or other training methods well known to those skilled in the art for shaking table.In the present invention, the mass ratio of described methane and oxygen is preferably 1:1 ~ 1.3.
After obtaining methane-oxidizing bacteria bacterium liquid, it mixed with mineralized waste, can obtain for refuse landfill, especially household refuse landfill sites tectum is to control the covering material of methane release.
Present invention also offers a kind of Covering Material of Refuse Landfill, comprising: the methane-oxidizing bacteria bacterium liquid that mineralized waste and the method described in technique scheme prepare.
The present invention is not particularly limited the described mineralized waste being used as Covering Material of Refuse Landfill, and be preferably the mineralized waste at 6 ages more than, its particle diameter is preferably 0.5mm ~ 4cm, and water ratio is preferably 20% ~ 30%.In described Covering Material of Refuse Landfill, described methane-oxidizing bacteria bacterium liquid accounts for the 8wt% ~ 12wt% of described mineralized waste, is preferably as 8.5wt% ~ 11.5wt%.
Covering Material of Refuse Landfill provided by the invention can directly overlay in refuse landfill and form tectum, by the methane oxidation in landfill gas, thus controls and reduce the release of methane.
Present invention also offers a kind of method of refuse landfill methane emission reduction, comprise the following steps:
The rubbish of landfill arranges tectum, and described tectum comprises the Covering Material of Refuse Landfill described in technique scheme.
Direct rubbish covering material described in technique scheme being covered landfill forms the release that tectum could control and reduce methane.In the present invention, described refuse landfill is preferably household refuse landfill sites, described covering material can, as covering in the middle of household refuse landfill sites or end covering, namely carry out at any time covering or covering after garbage loading embeading again in the process of landfill waste.
Described covering material is inserted in the landfill post of simulation landfill yard condition by the present invention, and pass into simulation landfill gas (volume ratio is the methane and carbon dioxide of 1:1) in landfill column bottom, landfill column top passes into air, detects covering material to the oxidation ratio of methane.Experimental result shows, the methane oxidation rate after covering material provided by the invention covers 7 days can reach more than 84%, and not only onset is rapid, and methane oxidation rate is higher.
The present invention originates using mineralized waste as methane-oxidizing bacteria strain, using percolate as culture medium mixed culture methane-oxidizing bacteria, culture system and landfill yard environment comparatively close, the methane-oxidizing bacteria bacterium liquid obtained more adapts to refuse landfill environment, and the methane-oxidizing bacteria bacterium liquid onset obtained is rapid, higher to the oxidation efficiency of methane in landfill gas, when covering 7 days, methane oxidation rate can reach more than 84%.Simultaneously, percolate and mineralized waste are waste, respectively using it as culture medium and bacterial classification source, solve the problem of environmental pollution that percolate causes, achieve the recycling of mineralized waste, not only method is simple, effectively reduces the cost of refuse landfill methane gas process, and effectively expand the landfill capacity of refuse landfill, extend the working life of refuse landfill.In addition, cover in refuse landfill as covering material after methane-oxidizing bacteria bacterium liquid provided by the invention mixes with mineralized waste, effectively by the methane oxidation in landfill gas, thus the discharge of methane of landfill yard can be controlled, reach the object of the treatment of wastes with processes of wastes against one another.
In order to further illustrate the present invention; be described below in conjunction with the method for embodiment to the preparation method of methane-oxidizing bacteria bacterium liquid provided by the invention, Covering Material of Refuse Landfill and refuse landfill methane emission reduction, protection scope of the present invention is not limited by the following examples.
Embodiment 1
Get the mineralized waste raw material at Shanghai Lao Gang household refuse landfill sites 10 age, removal of impurities obtains the fines that particle diameter is 0.5mm ~ 4cm after sieving, and water ratio is 26wt%.Described mineralized waste is loaded serum bottle, passes into the CH that volume ratio is 1:1
4and O
2mixed gas in 37 DEG C domestication 7d, now, the methane oxidation rate of mineralized waste reaches 56.8%, stop cultivate.
Added in described mineralized waste by the 10 age percolates taking from Shanghai Lao Gang household refuse landfill sites and obtain mixed solution, proportioning is 1g mineralized waste: 100mL percolate, and the pH value of mixed solution is 7.6; Mixed solution is loaded serum bottle, passes into the CH that volume ratio is 1:1
4and O
2mixed gas after seal, under 35 DEG C of conditions shaking table cultivate.Cultivate 3d, now, the cell concentration OD of mixed solution
600reach 0.76, stop cultivating, obtain methane-oxidizing bacteria bacterium liquid.
Described methane-oxidizing bacteria bacterium liquid is sprayed onto in mineralized waste, described methane-oxidizing bacteria bacterium liquid accounts for the 8wt% of mineralized waste, covering material made by the landfill post inserting simulation landfill yard condition after stirring, cover material thickness is 60cm, pass into simulation landfill gas in landfill column bottom, namely volume ratio is the CH of 1:1
4and CO
2, landfill gas load is 12mL/min; Landfill column top blowing air, the intake of air is 150mL/min, and methane oxidation effect is as shown in table 1.
The methane oxidation rate of Different Covering Material after table 17d
| Different Covering Material | Yellow earth | Mineralized waste | Mineralized waste+bacterium liquid |
| Methane oxidation rate (%) | 6 | 39 | 87 |
As shown in Table 1, covering material onset provided by the invention is rapid, and after 7 days, methane oxidation rate can reach 87%.
Embodiment 2
Get the mineralized waste raw material at Shanghai Lao Gang household refuse landfill sites 16 age, removal of impurities obtains the fines that particle diameter is 0.5mm ~ 4cm after sieving, and water ratio is 23%.Described mineralized waste is loaded serum bottle, passes into the CH of volume ratio 1:1.3
4and O
2in 37 DEG C of domestication 7d, now, the methane oxidation rate of mineralized waste reaches 55%, stops cultivating.
Added in described mineralized waste by Shanghai Lao Gang consumer waste landfill percolate mineralising bed process tail water and obtain mixed solution, proportioning is 0.5g mineralized waste: 100mL percolate, and the pH value of mixed solution is 7.5; Mixed solution is loaded serum bottle, passes into the CH that volume ratio is 1:1.3
4and O
2rear sealing, under 35 DEG C of conditions, shaking table is cultivated.Cultivate 3d, now, the cell concentration OD of mixed solution
600reach 0.82, stop cultivating, obtain methane-oxidizing bacteria bacterium liquid.
Described methane-oxidizing bacteria bacterium liquid is sprayed onto in mineralized waste, described methane-oxidizing bacteria bacterium liquid accounts for the 12wt% of mineralized waste, covering material made by the landfill post inserting simulation landfill yard condition after stirring, cover material thickness is 60cm, pass into simulation landfill gas in landfill column bottom, namely volume ratio is the CH of 1:1
4and CO
2, landfill gas load is 12mL/min; Landfill column top blowing air, the intake of air is 150mL/min, and when 3 days, methane oxidation rate reaches 84%, reaches 90% when 1 week.
Embodiment 3
Get the mineralized waste raw material at Shanghai Lao Gang household refuse landfill sites 11 age, removal of impurities obtains the fines that particle diameter is 0.5mm ~ 4cm after sieving, and water ratio is 28%.Described mineralized waste is loaded serum bottle, passes into the CH that volume ratio is 1:1.2
4and O
2in 37 DEG C of domestication 8d, now, the methane oxidation rate of mineralized waste reaches 53%, stops cultivating.
Shanghai Lao Gang consumer waste landfill percolate is recharged process tail water to add described mineralized waste and obtain mixed solution, proportioning is 1g mineralized waste: 100mL percolate, and the pH value of mixed solution is 7.2.Mixed solution is loaded serum bottle, passes into the CH that volume ratio is 1:1.2
4and O
2rear sealing, under 35 DEG C of conditions, shaking table is cultivated.Cultivate 3d, now, the cell concentration OD of mixed solution
600reach 0.77, stop cultivating, obtain methane-oxidizing bacteria bacterium liquid.
Described methane-oxidizing bacteria bacterium liquid is sprayed onto in mineralized waste, described methane-oxidizing bacteria bacterium liquid accounts for the 4wt% of mineralized waste, covering material made by the landfill post inserting simulation landfill yard condition after stirring, cover material thickness is 60cm, pass into simulation landfill gas in landfill column bottom, namely volume ratio is the CH of 1:1
4and CO
2, landfill gas load is 12mL/min; Landfill column top blowing air, the intake of air is 150mL/min, and when 1 week, methane oxidation rate reaches 86%.
Embodiment 4
Get the mineralized waste raw material at Shanghai Lao Gang household refuse landfill sites 16 age, removal of impurities obtains the fines that particle diameter is 0.5mm ~ 4cm after sieving, and water ratio is 23%.Described mineralized waste is loaded serum bottle, passes into the CH of volume ratio 1:1
4and O
2in 37 DEG C of domestication 7d, now, the methane oxidation rate of mineralized waste reaches 55%, stops cultivating.
Shanghai Lao Gang household refuse landfill sites 19 age percolate is added described mineralized waste, and proportioning is 0.5g mineralized waste: 100mL percolate, and the pH value of mixed solution is 7.3.Mixed solution is loaded serum bottle, passes into the CH of volume ratio 1:1
4and O
2rear sealing, under 35 DEG C of conditions, shaking table is cultivated.Cultivate 3d, now, the cell concentration OD of mixed solution
600reach 0.84, stop cultivating, obtain methane-oxidizing bacteria bacterium liquid.
Described methane-oxidizing bacteria bacterium liquid is sprayed onto in mineralized waste, described methane-oxidizing bacteria bacterium liquid accounts for the 8wt% of mineralized waste, covering material made by the landfill post inserting simulation landfill yard condition after stirring, cover material thickness is 60cm, pass into simulation landfill gas in landfill column bottom, namely volume ratio is the CH of 1:1
4and CO
2, landfill gas load is 12mL/min; Landfill column top blowing air, the intake of air is 150mL/min, and when 1 week, methane oxidation rate reaches 88%.
Comparative example 1
Get the mineralized waste at Shanghai Lao Gang household refuse landfill sites 16 age, removal of impurities obtains the fines that particle diameter is 0.5mm ~ 4cm after sieving, and water ratio is 23%; Get landfill 5 age, water ratio be 35% mineralising mud; By mineralized waste and mineralising mud according to 70:30(butt) mass ratio mixing, covering material made by the landfill post inserting simulation landfill yard condition after stirring, cover material thickness is 60cm, passes into simulation landfill gas in landfill column bottom, and namely volume ratio is the CH of 1:1
4and CO
2, landfill gas load is 12mL/min; Landfill column top blowing air, the intake of air is 150mL/min, and when 3 days, methane oxidation rate reaches 17%, reaches 19% when 1 week, reaches 76% when 1 month.
Comparative example 2
Disclosed in the Chinese patent literature embodiment 1 being 200910046444.8 according to application number, method prepares methyl hydride oxidized bacteria agent, described methyl hydride oxidized bacteria agent is joined in mineralized waste, described methane-oxidizing bacteria bacterium liquid accounts for the 12wt% of mineralized waste, covering material made by the landfill post inserting simulation landfill yard condition after stirring, cover material thickness is 60cm, pass into simulation landfill gas in landfill column bottom, namely volume ratio is the CH of 1:1
4and CO
2, landfill gas load is 12mL/min; Landfill column top blowing air, the intake of air is 150mL/min, and when 3 days, methane oxidation rate reaches 70%, reaches 78% when 1 week, reaches 80% when one month.
Comparative example 3
Get the mineralized waste raw material at Shanghai Lao Gang household refuse landfill sites 16 age, removal of impurities obtains the fines that particle diameter is 0.5mm ~ 4cm after sieving, and water ratio is 23%;
The starch solution of above-mentioned for 20g mineralized waste and 0.1g/L is mixed sealing in serum bottle, after mixing, covering material made by the landfill post inserting simulation landfill yard condition, and cover material thickness is 60cm, pass into simulation landfill gas in landfill column bottom, namely volume ratio is the CH of 1:1
4and CO
2, landfill gas load is 12mL/min; Landfill column top blowing air, the intake of air is 150mL/min, and when 3 days, methane oxidation rate reaches 15%, reaches 35% when 1 week, reaches 48% when one month.
Comparative example 4
Get the mineralized waste raw material at Shanghai Lao Gang household refuse landfill sites 16 age, removal of impurities obtains the fines that particle diameter is 0.5mm ~ 4cm after sieving, and water ratio is 23%.
Added in described mineralized waste by Shanghai Lao Gang consumer waste landfill percolate mineralising bed process tail water and obtain mixed solution, proportioning is 0.5g mineralized waste: 100mL percolate, and the pH value of mixed solution is 7.5; Mixed solution is loaded serum bottle, passes into the CH that volume ratio is 1:1.3
4and O
2rear sealing, under 35 DEG C of conditions, shaking table is cultivated.Cultivate 3d, obtain methane-oxidizing bacteria bacterium liquid.
Described methane-oxidizing bacteria bacterium liquid is sprayed onto in mineralized waste, described methane-oxidizing bacteria bacterium liquid accounts for the 12wt% of mineralized waste, covering material made by the landfill post inserting simulation landfill yard condition after stirring, cover material thickness is 60cm, pass into simulation landfill gas in landfill column bottom, namely volume ratio is the CH of 1:1
4and CO
2, landfill gas load is 12mL/min; Landfill column top blowing air, the intake of air is 150mL/min, and when 3 days, methane oxidation rate reaches 10%, reaches 23% when 1 week, reaches 60% when one month.
Comparative example 5
Get the mineralized waste raw material at Shanghai Lao Gang household refuse landfill sites 16 age, removal of impurities obtains the fines that particle diameter is 0.5mm ~ 4cm after sieving, and water ratio is 23%.Described mineralized waste is loaded serum bottle, passes into the CH of volume ratio 1:1.3
4and O
2in 37 DEG C of domestication 3d, now, the methane oxidation rate of mineralized waste reaches 35%, stops cultivating.
Added in described mineralized waste by Shanghai Lao Gang consumer waste landfill percolate mineralising bed process tail water and obtain mixed solution, proportioning is 0.5g mineralized waste: 100mL percolate, and the pH value of mixed solution is 7.5; Mixed solution is loaded serum bottle, passes into the CH that volume ratio is 1:1.3
4and O
2rear sealing, under 35 DEG C of conditions, shaking table is cultivated.Cultivate 3d, stop cultivating, obtain methane-oxidizing bacteria bacterium liquid.
Described methane-oxidizing bacteria bacterium liquid is sprayed onto in mineralized waste, described methane-oxidizing bacteria bacterium liquid accounts for the 12wt% of mineralized waste, covering material made by the landfill post inserting simulation landfill yard condition after stirring, cover material thickness is 60cm, pass into simulation landfill gas in landfill column bottom, namely volume ratio is the CH of 1:1
4and CO
2, landfill gas load is 12mL/min; Landfill column top blowing air, the intake of air is 150mL/min, and when 3 days, methane oxidation rate reaches 19%, reaches 35% when 1 week, reaches 80% when one month.
The above is only the preferred embodiment of the present invention; it should be pointed out that for those skilled in the art, under the premise without departing from the principles of the invention; can also make some improvements and modifications, these improvements and modifications also should be considered as protection scope of the present invention.
Claims (6)
1. a preparation method for methane-oxidizing bacteria bacterium liquid, comprises the following steps:
Mixed with percolate by mineralized waste, cultivate under passing into the condition of the mixed gas of methane and oxygen, obtain methane-oxidizing bacteria bacterium liquid, the methane oxidation rate of described mineralized waste is more than 50%;
Described mineralized waste carries out pre-treatment in accordance with the following methods:
The mixed gas passing into methane and oxygen in mineralized waste raw material is tamed, and tame and obtain mineralized waste to methane oxidation rate more than 50%, the temperature of described domestication is 28 DEG C ~ 37 DEG C;
Described mineralized waste raw material is the mineralized waste at 8 ages more than, and the particle diameter of described mineralized waste raw material is 0.5mm ~ 4cm, and the water ratio of described mineralized waste raw material is 20% ~ 30%;
Described percolate is percolate from garbage filling field, landfill percolate recharges process tail water or landfill percolate mineralising bed process tail water;
Described percolate is 8 age above percolates, in described percolate, total nitrogen content lower than 1400mg/L, Ni content lower than 0.4mg/L.
2. preparation method according to claim 1, is characterized in that, the mass volume ratio of described mineralized waste and described percolate is 0.5g ~ 2g:100mL.
3. preparation method according to claim 1, is characterized in that, the pH value of the mixed solution obtained after described mineralized waste and percolate mix is 7 ~ 9, and the temperature of described cultivation is 25 DEG C ~ 37 DEG C, is cultured to the cell concn OD of bacterium liquid
600more than 0.6.
4. preparation method according to claim 1, is characterized in that, when cultivating under passing into the condition of the mixed gas of methane and oxygen, the volume ratio of described methane and oxygen is 1:1 ~ 1.3.
5. a Covering Material of Refuse Landfill, comprising: the methane-oxidizing bacteria bacterium liquid that mineralized waste and the method described in any one of Claims 1 to 4 prepare, described methane-oxidizing bacteria bacterium liquid accounts for the 8wt% ~ 12wt% of described mineralized waste.
6. a method for refuse landfill methane emission reduction, comprises the following steps:
The rubbish of landfill arranges tectum, and described tectum comprises Covering Material of Refuse Landfill according to claim 5.
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| CN105861353A (en) * | 2016-01-11 | 2016-08-17 | 吉林大学 | Methane-oxidizing bacteria separated from dairy cow feces and separation method thereof |
| CN105798048A (en) * | 2016-03-18 | 2016-07-27 | 中国环境科学研究院 | Oxide layer material capable of adsorbing and degrading methane gas and preparation method and application of oxide layer material |
| CN107996057A (en) * | 2017-12-05 | 2018-05-08 | 中国科学院青岛生物能源与过程研究所 | A kind of method for combining soil water-retaining soil moisture conservation and emission reduction methane |
| CN109465275B (en) * | 2018-10-24 | 2022-07-12 | 贵州欧瑞欣合环保股份有限公司 | Sludge-based refuse landfill covering material |
| CN109465274B (en) * | 2018-10-24 | 2021-08-03 | 贵州欧瑞欣合环保股份有限公司 | Refuse landfill covering material and preparation method thereof |
| CN110452846B (en) * | 2019-08-16 | 2022-07-12 | 鹭滨环保科技（上海）股份有限公司 | Paracoccus denitrificans and biological denitrification method by combining paracoccus denitrificans with mineralization bed |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN101062848A (en) * | 2007-04-30 | 2007-10-31 | 同济大学 | Methane oxidation covering material for life refuse landfill |
| CN101492642A (en) * | 2009-02-23 | 2009-07-29 | 同济大学 | Process for producing methyl hydride oxidized bacteria agent |
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Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101062848A (en) * | 2007-04-30 | 2007-10-31 | 同济大学 | Methane oxidation covering material for life refuse landfill |
| CN101492642A (en) * | 2009-02-23 | 2009-07-29 | 同济大学 | Process for producing methyl hydride oxidized bacteria agent |
Non-Patent Citations (1)
| Title |
|---|
| 矿化垃圾处理垃圾渗滤液的试验研究;陶正望等;《环境科学研究》;20081231;第21卷(第6期);26-29 * |
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