CN103495018B - The preparation method of the raw arteries and veins oral liquid of a kind of restoring consciouness - Google Patents
The preparation method of the raw arteries and veins oral liquid of a kind of restoring consciouness Download PDFInfo
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Abstract
The preparation method of the raw arteries and veins oral liquid of a kind of restoring consciouness, it relates to the preparation method of a kind of oral liquid. The present invention to be solved containing caffeine in existing Antifatigue oral liquid, the technical problem that effect is single. The method of the present invention is: one, the preparation of liquid at the bottom of SHENGMAI YIN: Radix Codonopsis, the tuber of dwarf lilyturf, shizandra berry being decocted and boiled, filtration, merge decoction liquor, then take out filter, normal pressure concentrates, and cooling, adds dehydrated alcohol, reconcentration, and fixed appearance, namely obtains liquid at the bottom of SHENGMAI YIN; Two, prepared by the raw arteries and veins oral liquid of restoring consciouness: gets liquid at the bottom of SHENGMAI YIN, then adds Radix Ginseng total saponins, tea-polyphenol, taurine, sucrose, vitamins C, potassium sorbate, filters after fully dissolving, fixed appearance, obtains the raw arteries and veins oral liquid of restoring consciouness. The anti-fatigue effect of SHENGMAI YIN, Radix Ginseng total saponins, taurine and tea-polyphenol is combined by the raw arteries and veins oral liquid of restoring consciouness prepared by the present invention, and these three kinds of compositions had not both had antagonistic action and not containing caffeine material, had been free from side effects by health. The present invention is applied to the preparation field of oral liquid.
Description
Technical field
The present invention relates to the preparation method of a kind of oral liquid.
Background technology
In current society, people, due to the pressure of work, study, life etc., very easily produce tired state, this promotes the generation of anti-fatigue health-product containing. Meanwhile, along with improving constantly of standard of living, the health care of daily life is also more and more paid attention to by people, and various healthcare products also arise at the historic moment.
At present, ginsenoside Antifatigue oral liquid and SHENGMAI YIN KOUFUYE are all compare common oral liquid on the market. But, effect of these two kinds of products is more single, and a lot of healthcare products all contain caffeine. So multi-functional, just become the product innovation that people expect without the oral liquid of caffeine.
Radix Ginseng total saponins is one of ginseng main active ingredient, there is the rising suppressing tryptophane content in cerebral tissue, thus suppress the generation of serotonin, delay central nerve fatigue to occur, Radix Ginseng total saponins by suppressing hydroxyl oxyradical to produce, alleviate the effect of the approach performance antioxidant radical such as the oxidative damage of radical pair cytolipin composition and the removing of acceleration free radical, thus can reach the object of alleviating physical fatigue simultaneously. In addition, Radix Ginseng total saponins by strengthening the activity of LDH, can accelerate the removing of lactic acid, and then reduce the accumulation of lactic acid, promote tired recovery;
Taurine is a kind of beta amino acids containing sulphur, as conditionally essential amino acid a kind of in human body, to the freedom in motion body and metabolism, Ca2+Transhipment etc. has good regulating effect, thus shows good anti-exercise fatigue effect;
Tea-polyphenol and principal monomer catechin thereof as a kind of natural antioxidants, its efficiency scavenging free radicals of the polyphenol hydroxyl structures shape that it has, delayed motion is tired, improve human motion ability and safeguard that sportsmen's health will play a positive role.
" SHENGMAI YIN " is famous ancient prescriptions in thousand, mainly has functions such as " Yiqi and vein recovery, recuperating deplered YANG and rescuing the patient from collapse ", and " SHENGMAI YIN " has anti peroxidation of lipid, protection cardiac muscle, the effect strengthening heart function and enhancing development and learning and memory.
Summary of the invention
The present invention to be solved containing caffeine in existing Antifatigue oral liquid, the technical problem that effect is single, thus provides the preparation method of the raw arteries and veins oral liquid of a kind of restoring consciouness.
The preparation method of the raw arteries and veins oral liquid of a kind of restoring consciouness of the present invention carries out according to the following steps:
One, the preparation of liquid at the bottom of SHENGMAI YIN: the shizandra berry of the tuber of dwarf lilyturf of the Radix Codonopsis of 20��40g, 10��30g, 5��15g is decocted and boils 2 times, filter, merge decoction liquor, obtain extracting solution, extracting solution filter flask is taken out filter, and filtrate normal pressure concentrates to 30mL, cooling, add 50��dehydrated alcohol of 80mL, place 12��24h, then concentrate with Rotary Evaporators, make filtrate be condensed into paste, it is settled to 100mL, namely obtains liquid at the bottom of SHENGMAI YIN; Wherein first time decocts consumption to boil water is 500��800mL, and decocting time is 1.5��3h, and it is 400��600mL that second time decocts consumption to boil water, and backflow decocting time is 1��2h;
Two, the raw arteries and veins oral liquid preparation of restoring consciouness: get liquid at the bottom of the SHENGMAI YIN that 30��50mL step one obtains, then Radix Ginseng total saponins is added, tea-polyphenol, taurine, the sucrose of 9.5��10.8g, the vitamins C of 10��30mg, the potassium sorbate of 10��20mg, filter after fully dissolving, namely obtain the raw arteries and veins oral liquid of restoring consciouness; Wherein, the mass ratio of described Radix Ginseng total saponins, tea-polyphenol and taurine is 1��3: 1��4: 1��3.
The present invention comprises following useful effect:
The anti-fatigue effect of the anti-fatigue effect of SHENGMAI YIN with Radix Ginseng total saponins, taurine and tea-polyphenol is combined by the present invention, and these three kinds of compositions had not both had antagonistic action and not containing caffeine material, had been free from side effects by health; Meanwhile, also there are brain tonic and intelligence development, immunomodulatory, antitumor, radioprotective, anti-dental caries deodorizing, the carbohydrate metabolism and lipid metabolism, cytoprotective, anti-arrhythmia, hypertension and atherosclerosis, the heart failure resistance that regulate body, the effect such as improve visual signalling conduction; Add ascorbic mode to develop, reduce the oxidation of tea-polyphenol, develop the Antifatigue oral liquid without caffeine.
Accompanying drawing explanation
Fig. 1 is the raw arteries and veins oral liquid anti-fatigue effect time verifying test mice swimming time result of restoring consciouness; Wherein, �� is experimental group, and �� is control group;
Fig. 2 is the raw arteries and veins oral liquid anti-fatigue effect time verifying test mice blood lactase acid result of restoring consciouness; Wherein, �� is experimental group, and �� is control group;
Fig. 3 is the raw arteries and veins oral liquid anti-fatigue effect time verifying test mice serum urea result of restoring consciouness; Wherein, �� is experimental group, and �� is control group.
Embodiment
Embodiment one: the preparation method of the raw arteries and veins oral liquid of a kind of restoring consciouness of present embodiment carries out according to the following steps:
One, the preparation of liquid at the bottom of SHENGMAI YIN: the shizandra berry of the tuber of dwarf lilyturf of the Radix Codonopsis of 20��40g, 10��30g, 5��15g is decocted and boils 2 times, filter, merge decoction liquor, obtain extracting solution, extracting solution filter flask is taken out filter, and filtrate normal pressure concentrates to 30mL, cooling, add 50��dehydrated alcohol of 80mL, place 12��24h, then concentrate with Rotary Evaporators, make filtrate be condensed into paste, it is settled to 100mL, namely obtains liquid at the bottom of SHENGMAI YIN; Wherein first time decocts consumption to boil water is 500��800mL, and decocting time is 1.5��3h, and it is 400��600mL that second time decocts consumption to boil water, and decocting time is 1��2h;
Two, the raw arteries and veins oral liquid preparation of restoring consciouness: get liquid at the bottom of the SHENGMAI YIN that 30��50mL step one obtains, then Radix Ginseng total saponins is added, tea-polyphenol, taurine, the sucrose of 9.5��10.8g, the vitamins C of 10��30mg, the potassium sorbate of 10��20mg, filter after fully dissolving, namely obtain the raw arteries and veins oral liquid of restoring consciouness; Wherein, the mass ratio of described Radix Ginseng total saponins, tea-polyphenol and taurine is 1��3: 1��4: 1��3.
Present embodiment comprises following useful effect:
The anti-fatigue effect of the anti-fatigue effect of SHENGMAI YIN with Radix Ginseng total saponins, taurine and tea-polyphenol is combined by present embodiment, and these three kinds of compositions had not both had antagonistic action and not containing caffeine material, had been free from side effects by health; Meanwhile, also there are brain tonic and intelligence development, immunomodulatory, antitumor, radioprotective, anti-dental caries deodorizing, the carbohydrate metabolism and lipid metabolism, cytoprotective, anti-arrhythmia, hypertension and atherosclerosis, the heart failure resistance that regulate body, the effect such as improve visual signalling conduction; Add ascorbic mode to develop, reduce the oxidation of tea-polyphenol, develop the Antifatigue oral liquid without caffeine.
Embodiment two: present embodiment and embodiment one the difference is that: step one is placed 24h. Other is identical with embodiment one.
Embodiment three: present embodiment and embodiment one or two the difference is that: in step one, first time decocts consumption to boil water is 600mL. Other is identical with embodiment one or two.
Embodiment four: one of present embodiment and embodiment one to three the difference is that: in step one first time decocting time be 2h. One of other and embodiment one to three are identical.
Embodiment five: one of present embodiment and embodiment one to four the difference is that: in step one second time decoct consumption to boil water be 500mL. One of other and embodiment one to four are identical.
Embodiment six: one of present embodiment and embodiment one to five the difference is that: in step one second time decocting time be 1.5h. One of other and embodiment one to five are identical.
The useful effect of the present invention is verified by following examples:
Embodiment one: the preparation method of the raw arteries and veins oral liquid of a kind of restoring consciouness of the present embodiment realizes according to the following steps:
One, the preparation of liquid at the bottom of SHENGMAI YIN: the shizandra berry of the tuber of dwarf lilyturf of the Radix Codonopsis of 30g, 20g, 10g is decocted and boils 2 times, filter, merge decoction liquor, obtain extracting solution, extracting solution filter flask is taken out filter, and filtrate normal pressure concentrates to 30mL, cooling, add the ethanol of 60mL, place 24h, then concentrate with Rotary Evaporators, make filtrate be condensed into paste, it is settled to 100mL, namely obtains liquid at the bottom of SHENGMAI YIN; Wherein first time decocts consumption to boil water is 600mL, and decocting time is 2h, and it is 500mL that second time decocts consumption to boil water, and decocting time is 1.5h;
Two, the raw arteries and veins oral liquid preparation of restoring consciouness: get liquid at the bottom of the SHENGMAI YIN that 30mL step one obtains; then the Radix Ginseng total saponins of 5g is added; the tea-polyphenol of 5g; the taurine of 5g, the sucrose of 10.2g, the vitamins C of 20mg; the potassium sorbate of 16mg; filter after fully dissolving, it is settled to 40mL, namely obtain the raw arteries and veins oral liquid of restoring consciouness.
Embodiment two: the present embodiment and embodiment one are the difference is that the Radix Ginseng total saponins adding 5g in step 2, the tea-polyphenol of 10g, the taurine of 10g.
Embodiment three: the present embodiment and embodiment one are the difference is that the Radix Ginseng total saponins adding 5g in step 2, the tea-polyphenol of 20g, the taurine of 15g.
Embodiment four: the present embodiment and embodiment one are the difference is that the Radix Ginseng total saponins adding 10g in step 2, the tea-polyphenol of 5g, the taurine of 10g.
Embodiment five: the present embodiment and embodiment one are the difference is that the Radix Ginseng total saponins adding 10g in step 2, the tea-polyphenol of 10g, the taurine of 15g.
Embodiment six: the present embodiment and embodiment one are the difference is that the Radix Ginseng total saponins adding 10g in step 2, the tea-polyphenol of 20g, the taurine of 5g.
Embodiment seven: the present embodiment and embodiment one are the difference is that the Radix Ginseng total saponins adding 15g in step 2, the tea-polyphenol of 5g, the taurine of 15g.
Embodiment eight: the present embodiment and embodiment one are the difference is that the Radix Ginseng total saponins adding 15g in step 2, the tea-polyphenol of 10g, the taurine of 5g.
Embodiment nine: the present embodiment and embodiment one are the difference is that the Radix Ginseng total saponins adding 15g in step 2, the tea-polyphenol of 20g, the taurine of 10g.
Test one: restoring consciouness oral liquid functional substance formulating
KM mouse, in 6��8 week age, body weight 18��22g, feeds in 21��22 DEG C of environment, first adapts to environment 3��7d, random packet, often organizes 12 (6 ��, 6 ��). Determine the optimal dose level of three kinds of primary efficacy materials with embodiment one to embodiment nine after, set up orthogonal test table 1.
Table 1
Every morning 9:00��10:00 gavage, blank group (physiological saline of equal solvent), every day gastric infusion, continue 14d. In addition, between trial period, mouse freely ingests and drinks water, and every day changes water, within every two days, changes bedding and padding. After last gives given the test agent 30min, the mouse of tail root load 5% body weight sheet lead is placed in swimming trunk went swimming (depth of water is no less than 30cm, water temperature 25 �� 1 DEG C), exhausts to mouse swimming power, pull mouse out. Mouse swimming power is plucked eye immediately and is got blood, centrifuging and taking serum, survey mouse lactic acid content according to kit method after exhausting. The results are shown in Table 2.
Table 2
Note: the 10th group is negative control experiments group; * represent and negative control experiments group significant difference.
Table 2 illustrates that the body weight of mouse does not significantly change in the gavage process of 14 days, but compared with control group (the 10th group), the Serum lactic acid content of experimental group 3��9 all has reduction in various degree, proves that these formulas organized all have the effect of antifatigue.
Embodiment ten: the preparation method of the raw arteries and veins oral liquid of a kind of restoring consciouness of the present embodiment realizes according to the following steps:
One, the preparation of liquid at the bottom of SHENGMAI YIN: the shizandra berry of the tuber of dwarf lilyturf of the Radix Codonopsis of 30g, 20g, 10g is decocted and boils 2 times, filter, merge decoction liquor, obtain extracting solution, extracting solution filter flask is taken out filter, and filtrate normal pressure concentrates to 30mL, cooling, add the ethanol of 60mL, place 24h, then concentrate with Rotary Evaporators, make filtrate be condensed into paste, it is settled to 100mL, namely obtains liquid at the bottom of SHENGMAI YIN; Wherein first time decocts consumption to boil water is 600mL, and decocting time is 2h, and it is 500mL that second time decocts consumption to boil water, and decocting time is 1.5h;
Two, the raw arteries and veins oral liquid preparation of restoring consciouness: get liquid at the bottom of the SHENGMAI YIN that 30mL step one obtains; then the Radix Ginseng total saponins of 0.9g is added; the tea-polyphenol of 0.3g; the taurine of 0.9g, the sucrose of 10.2g, the vitamins C of 20mg; the potassium sorbate of 16mg; filter after fully dissolving, it is settled to 40mL, namely obtain the raw arteries and veins oral liquid of restoring consciouness.
The raw arteries and veins oral liquid of 40mL restoring consciouness prepared by the present embodiment is the human body dose of a day.
Embodiment 11: the present embodiment and embodiment ten are the difference is that the Radix Ginseng total saponins adding 9g in step 2, the tea-polyphenol of 3g, the taurine of 9g.
Embodiment 12: the present embodiment and embodiment ten are the difference is that the Radix Ginseng total saponins adding 4.5g in step 2, the tea-polyphenol of 1.5g, the taurine of 4.5g.
Embodiment 13: the present embodiment and embodiment one are the difference is that the Radix Ginseng total saponins adding 1.8g in step 2, the tea-polyphenol of 0.6g, the taurine of 1.8g.
Test two: the raw arteries and veins oral liquid various dose anti-fatigue effect proof test of restoring consciouness
Kunming mice, male, 6��8 week age, body weight 18��22g, feed in 21��22 DEG C of environment, first adapt to environment 3��7d, it is divided into three dosage groups and a negative control group at random, taking embodiment 11 as a dosage group, it is designated as high dose group (H), taking embodiment 12 as a dosage group, it is designated as middle dosage group (M), taking embodiment 13 as a dosage group, it is designated as low dose group (L), the physiological saline (physiological saline of 40mL) of equal solvent, is designated as negative control group (C), often organizes 6.
Every morning 9:00��10:00 gavage, every day gastric infusion, continue 30d. In addition, between trial period, mouse freely ingests and drinks water, and every day changes water, within every two days, changes bedding and padding. After last gives given the test agent 30min, the mouse of tail root load 5% body weight sheet lead is placed in swimming trunk went swimming (depth of water is no less than 30cm, water temperature 25 �� 1 DEG C), and record mouse starts to sink to 8s at the bottom of case from swimming and can not float voluntarily, record is the mice burden swimming time, pulls mouse out. Mouse swimming power is plucked eye immediately and is got blood, centrifuging and taking serum after exhausting, and surveys mouse lactic acid and serum urea content according to kit method. The results are shown in Table 3.
Table 3
Table 3 result shows, three dosage groups are not remarkable on the impact of mouse body weight, but the raising along with dosage, and the time of mouse swimming extends gradually, and the content of blood lactase acid and serum urea declines gradually simultaneously.
Test three: the raw arteries and veins oral liquid antifatigue time verifying test of restoring consciouness
Kunming mice, in male 6��8 week age, body weight 18��22g, feeds in 21��22 DEG C of environment, first adapts to environment 3��7d, and random 5 groupings (often organizing 6), are designated as A, B, C, D, E respectively. Namely represent and after giving given the test agent 30min, 1h, 2h, 4h, 8h, start anti-fatigue effect experiment.
The morning 9:00��10:00 gavage, the tested material of experimental group is the raw arteries and veins oral liquid of restoring consciouness, and the tested material of control group is the physiological saline of equal solvent. Anti-fatigue effect experiment is started respectively after giving given the test agent 30min, 1h, 2h, 4h, 8h. Being placed in swimming trunk went swimming (depth of water is no less than 30cm, water temperature 25 �� 1 DEG C) by the mouse of tail root load 5% body weight sheet lead, record mouse starts to sink to 8s at the bottom of case from swimming and can not float voluntarily, notes down as the mice burden swimming time, pulls mouse out. Mouse swimming power is plucked eye immediately and is got blood, centrifuging and taking serum after exhausting, and surveys mouse lactic acid and serum urea content according to kit method. The results are shown in Figure 1, Fig. 2, Fig. 3.
The raw arteries and veins oral liquid anti-fatigue effect time verifying test mice swimming time result of restoring consciouness is as shown in Figure 1; Wherein, �� is experimental group, and �� is control group; As can be seen from Figure 1, this oral liquid has good assimilation effect, and the time of gavage 30 minutes mice burden swimmings is significantly improved compared with control group, and sustainable 8 hours of this effect;
The raw arteries and veins oral liquid anti-fatigue effect time verifying test mice blood lactase acid result of restoring consciouness is as shown in Figure 2; Wherein, �� is experimental group, and �� is control group; As can be seen from Figure 2, this oral liquid has good assimilation effect, Serum lactic acid content obvious decline compared with control group after gavage 30 minutes mice burden swimmings, and sustainable 8 hours of this effect;
The raw arteries and veins oral liquid anti-fatigue effect time verifying test mice serum urea result of restoring consciouness is as shown in Figure 3; Wherein, �� is experimental group, and �� is control group; As can be seen from Figure 3, this oral liquid has good assimilation effect, serum urea nitrogen content obvious decline compared with control group after gavage 30 minutes mice burden swimmings, and sustainable 8 hours of this effect.
Claims (1)
1. the preparation method of the raw arteries and veins oral liquid of restoring consciouness, it is characterised in that the preparation method of the raw arteries and veins oral liquid of restoring consciouness carries out according to the following steps:
One, the preparation of liquid at the bottom of SHENGMAI YIN: the shizandra berry of the tuber of dwarf lilyturf of the Radix Codonopsis of 30g, 20g, 10g is decocted and boils 2 times, filter, merge decoction liquor, obtain extracting solution, extracting solution filter flask is taken out filter, and filtrate normal pressure concentrates to 30mL, cooling, add the ethanol of 60mL, place 24h, then concentrate with Rotary Evaporators, make filtrate be condensed into paste, it is settled to 100mL, namely obtains liquid at the bottom of SHENGMAI YIN; Wherein first time decocts consumption to boil water is 600mL, and decocting time is 2h, and it is 500mL that second time decocts consumption to boil water, and decocting time is 1.5h;
Two, the raw arteries and veins oral liquid preparation of restoring consciouness: get liquid at the bottom of the SHENGMAI YIN that 30mL step one obtains; then the Radix Ginseng total saponins of 15g is added; the tea-polyphenol of 5g; the taurine of 15g, the sucrose of 10.2g, the vitamins C of 20mg; the potassium sorbate of 16mg; filter after fully dissolving, it is settled to 40mL, namely obtain the raw arteries and veins oral liquid of restoring consciouness.
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| CN102078503A (en) * | 2009-11-30 | 2011-06-01 | 江西济民可信药业有限公司 | Detection method for pulse-activating decoction traditional Chinese medicine preparation |
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| CN102078503A (en) * | 2009-11-30 | 2011-06-01 | 江西济民可信药业有限公司 | Detection method for pulse-activating decoction traditional Chinese medicine preparation |
| CN102318866A (en) * | 2011-08-08 | 2012-01-18 | 桂林莱茵生物科技股份有限公司 | Beverage with effect of alleviating physical fatigue |
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