CN103484568A - Method and kit for dual fluorescence quantitative PCR detection of grass carp reovirus types I and II - Google Patents
Method and kit for dual fluorescence quantitative PCR detection of grass carp reovirus types I and II Download PDFInfo
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- CN103484568A CN103484568A CN201310430466.0A CN201310430466A CN103484568A CN 103484568 A CN103484568 A CN 103484568A CN 201310430466 A CN201310430466 A CN 201310430466A CN 103484568 A CN103484568 A CN 103484568A
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CN201310430466.0A CN103484568B (en) | 2013-09-18 | 2013-09-18 | Method and kit for dual fluorescence quantitative PCR detection of grass carp reovirus types I and II |
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CN201310430466.0A CN103484568B (en) | 2013-09-18 | 2013-09-18 | Method and kit for dual fluorescence quantitative PCR detection of grass carp reovirus types I and II |
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Cited By (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN106811550A (en) * | 2017-03-11 | 2017-06-09 | 中国水产科学研究院珠江水产研究所 | A kind of type vaccine strain of GCRV II and street strain's diagnostic primerses and kit and diagnosis detecting method containing it |
CN108342513A (en) * | 2018-05-10 | 2018-07-31 | 中国水产科学研究院长江水产研究所 | A kind of universal RT-PCR detection primers of different genotype grass carp reovirus and application |
CN109971890A (en) * | 2019-04-30 | 2019-07-05 | 上海海洋大学 | The droplet type digital pcr detection kit and its primer special and probe of II type grass carp reovirus |
CN110257385A (en) * | 2019-07-04 | 2019-09-20 | 广西科学院 | Anti- grass carp exhales aptamer and its construction method and the application of the lonely I type virus of intestines |
CN110894551A (en) * | 2018-09-13 | 2020-03-20 | 杭州众测生物科技有限公司 | RAA constant-temperature fluorescence detection method and reagent for grass carp hemorrhagic disease type I virus (GCRV-I) |
Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN102367486A (en) * | 2011-01-25 | 2012-03-07 | 中国水产科学研究院珠江水产研究所 | Reverse transcription-polymerase chain reaction (RT-PCR) fast detection reagent kit and detection method for grass carp hemorrhage disease pathogeny of grass carp reovirus |
CN102534050A (en) * | 2012-02-21 | 2012-07-04 | 中国水产科学研究院珠江水产研究所 | Triple polymerase chain reaction (PCR) detecting primer group, reagent kit and method for diagnosing grass carp reovirus |
-
2013
- 2013-09-18 CN CN201310430466.0A patent/CN103484568B/en not_active Expired - Fee Related
Patent Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN102367486A (en) * | 2011-01-25 | 2012-03-07 | 中国水产科学研究院珠江水产研究所 | Reverse transcription-polymerase chain reaction (RT-PCR) fast detection reagent kit and detection method for grass carp hemorrhage disease pathogeny of grass carp reovirus |
CN102534050A (en) * | 2012-02-21 | 2012-07-04 | 中国水产科学研究院珠江水产研究所 | Triple polymerase chain reaction (PCR) detecting primer group, reagent kit and method for diagnosing grass carp reovirus |
Non-Patent Citations (2)
Title |
---|
刘宝芹等: "草鱼呼肠孤病毒HZ08株FQ-PCR检测方法的建立及应用", 《中国水产科学》 * |
殷亮等: "水生呼肠孤病毒基因组研究进展", 《动物医学进展》 * |
Cited By (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN106811550A (en) * | 2017-03-11 | 2017-06-09 | 中国水产科学研究院珠江水产研究所 | A kind of type vaccine strain of GCRV II and street strain's diagnostic primerses and kit and diagnosis detecting method containing it |
CN108342513A (en) * | 2018-05-10 | 2018-07-31 | 中国水产科学研究院长江水产研究所 | A kind of universal RT-PCR detection primers of different genotype grass carp reovirus and application |
CN108342513B (en) * | 2018-05-10 | 2020-09-29 | 中国水产科学研究院长江水产研究所 | Universal RT-PCR (reverse transcription-polymerase chain reaction) detection primer for grass carp reoviruses with different genotypes and application |
CN110894551A (en) * | 2018-09-13 | 2020-03-20 | 杭州众测生物科技有限公司 | RAA constant-temperature fluorescence detection method and reagent for grass carp hemorrhagic disease type I virus (GCRV-I) |
CN109971890A (en) * | 2019-04-30 | 2019-07-05 | 上海海洋大学 | The droplet type digital pcr detection kit and its primer special and probe of II type grass carp reovirus |
CN110257385A (en) * | 2019-07-04 | 2019-09-20 | 广西科学院 | Anti- grass carp exhales aptamer and its construction method and the application of the lonely I type virus of intestines |
CN110257385B (en) * | 2019-07-04 | 2022-06-17 | 广西科学院 | Aptamer for resisting grass carp reovirus I, and construction method and application thereof |
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CN103484568B (en) | 2015-06-24 |
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