CN103484525A - Method, substrate and reagents for alpha-N-acetylglucosaminidase activity detection - Google Patents
Method, substrate and reagents for alpha-N-acetylglucosaminidase activity detection Download PDFInfo
- Publication number
- CN103484525A CN103484525A CN201210195780.0A CN201210195780A CN103484525A CN 103484525 A CN103484525 A CN 103484525A CN 201210195780 A CN201210195780 A CN 201210195780A CN 103484525 A CN103484525 A CN 103484525A
- Authority
- CN
- China
- Prior art keywords
- alpha
- acetamino
- glucose
- reagent
- polyglucosidase
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Landscapes
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
The present invention provides a method for alpha-N-acetylglucosaminidase (EC3.2.1.50) activity detection, and further provides a substrate and reagents for alpha-N-acetylglucosaminidase activity determination. The method, the substrate and the reagents can be used for analyzing and determining alpha-N-acetylglucosaminidase activity in a sample (including human body fluid or tissue or cell samples) requiring determination, and are mainly used in the field of clinical laboratory. The determination reagent prepared by using the method has characteristics of convenience, rapidness and high sensitivity, and is easily promoted and applied.
Description
Technical field
The invention belongs to the biological medicine technology field, belong to again the clinical diagnosis detection field of Inherited Metabolic Disorders simultaneously.Particularly the invention provides a kind of mensuration alpha-N-acetamino Polyglucosidase (α-N-acetylglucosaminidase, EC 3.2.1.50) method of enzyme activity, the present invention simultaneously also provides a kind of as alpha-N-acetamino Polyglucosidase (α-N-acetylglucosaminidase, EC 3.2.1.50) substrate that enzymic activity detects, the present invention also provides the reagent of mensuration alpha-N-acetamino Polyglucosidase (α-N-acetylglucosaminidase, EC 3.2.1.50) enzymic activity in addition.
Background technology
Lysosome is a kind of organoid by the unit membrane parcel in human body cell, and multiple acid hydrolase is contained in inside, and that has found at present has a kind more than 60, as enzymes such as proteolytic enzyme, nuclease, Glycosylase, lipase, Phosphoric acid esterase, N,O-Diacetylmuramidases.In cell, lysosomal enzyme is being controlled the digestion of multiple endogenous and exogenous macromolecular substance, as nucleic acid, protein, lipid, polysaccharide and glycogen etc., therefore, the lysosome intracellular digestion organs that are otherwise known as.The metabolism of human body every day can produce the unwanted macromolecular substance of many human bodies, as sphingolipid, glycoprotein, sticky fat, mucopolysaccharide, oligosaccharides, glycogen etc., these macromolecular substance need be hydrolyzed into small-molecule substance after lysosomal enzyme is processed can the sustainable participation organism metabolism or excrete.If in lysosome, the activity decreased of certain lytic enzyme or disappearance will cause specific biomacromolecule can not normally degrade and store up in lysosome, thereby make lysosome generation swelling, it is too fat to move not normal that cell becomes, cell function is had a strong impact on, finally cause a series of diseases, this class disease is referred to as lysosomal storage disease (Lysosomal Storage Diseases is called for short LSDs).According to the macromolecular substance kind difference of storing up, lysosomal storage disease is divided into to following several main Types: mucopolysaccharidosis, glycogen storage disease II type, glutinous lipoidosis, sphingolipid thesaurismosis, glycoprotein are stored up disease etc.
According to American-European countries's rough estimates data, show, lysosomal storage disease is done as a whole, and in the newborn infant, morbidity can be up to 1/8,000～1/5, and 000.Not yet carry out the examination statistics in China at present, but, along with the raising of biochemistry detection technology, the recall rate of domestic lysosomal storage disease obviously improves, and is progressively ascendant trend.
Mucopolysaccharidosis (mucopolysaccharidosis, MPS) be one group of lysosomal storage disease, be due to some lytic enzyme defect in lysosome, cause acidic mucopolysaccharide (glycosaminoglycan) degraded to be obstructed to cause mucopolysaccharide to gather in vivo and cause the disease of a series of clinical symptom.
Mucopolysaccharidosis belongs to congenital or primary metabolic disturbance syndrome.According to the kind of contained acidic mucopolysaccharide in glucose in urine, relevant indivedual enzymatic defects or active low kind and the difference of clinical manifestation and Radiologic imaging, be divided into 7 large types by mucopolysaccharidosis, is respectively:
MPS-I、MPS-II、MPS-III、MPS-IV、MPS-VI、MPS-VII、MPS-IX。
Mucopolysaccharidosis IIIB(MPS-IIIB) type is autosomal recessive disease, be due to alpha-N-acetamino Polyglucosidase (α-N-acetylglucosaminidase) hypofunction or due to lacking, with MPS-III A, MPS-III C and MPS-III D, belong to MPS-III type.
Mucopolysaccharidosis IIIB type is made a definite diagnosis difficulty and is shown many-sided: because of this disease, be at first multisystem disease, relate to organ or the systems such as heredity, nerve, muscle, blood, bone, eyes, the Symptoms complexity, and to multiple common disease, similar clinical manifestation is arranged, therefore the doctor is difficult to accumulate diagnostic experiences from Symptoms, therefore fail to pinpoint a disease in diagnosis, mistaken diagnosis and delay diagnosis be ubiquitous problem.The supplementary meanss such as next existing every routine inspection of hospital all are difficult to reach the purpose of making a definite diagnosis, as many inspection methods such as bone marrow aspiration, physical examination, electrocardiogram(ECG, electroencephalogram, B ultrasonic, CT/ nuclear-magnetism, x-ray, muscle biopsy, serum creatine phosphokinase inspection all can't be analyzed the real paathogenic factor of mucopolysaccharidosis IIIB type, therefore can not make Accurate Diagnosis to patient disease.Gene test is difficult, and because of the existence of mononucleotide sequence polymorphism, possible some sudden change is exactly normal, can be not diseases induced, therefore to analysis, bring uncertainty; Because the activity of enzyme is also relevant with the gene of some other participation enzyme function, the also not studied personnel's discovery at present of a lot of these genoids is arranged, so gene test equally can not be as the reliable basis of diagnosis in addition.
The direct factor that causes mucopolysaccharidosis IIIB type to produce is exactly that the interior single alpha-N-acetamino glucosidase activity of patient body reduces or lacks fully, and coincident with severity degree of condition is often closely related with the residual ratio of enzymic activity, therefore judge that whether the activity of patient's alpha-N-acetamino Polyglucosidase is normal, in conjunction with patient's clinical symptom performance, just can make diagnosis to patient's disease rapidly and accurately simultaneously.Therefore the diagnosis basis that the alpha-N-acetamino glucosidase activity detects as mucopolysaccharidosis IIIB type has the advantage that additive method can not be compared, clinically by doubtful mucopolysaccharidosis IIIB type patient is carried out to the detection of alpha-N-acetamino glucosidase activity, can realize early diagnosis, early treatment, to the prognosis of mucopolysaccharidosis IIIB type, reduce disease significant to family and social impact and the hidden danger of bringing.It is auxiliary this sick best means of making a definite diagnosis that the proof of clinical practice both at home and abroad detects the alpha-N-acetamino glucosidase activity.The present invention just is based on these technical backgrounds and social reality demand, characteristics according to the enzymatic activity of alpha-N-acetamino Polyglucosidase, the synthetic alpha-N-acetamino glucose derivative that contains fluorophor of design is as the substrate of enzymic catalytic reaction, thus the alpha-N-acetamino Polyglucosidase enzyme activity in can the mensuration sample to be tested of rapid sensitive.Alpha-N-acetamino Polyglucosidase in sample to be tested can will discharge fluorescence molecule and alpha-N-acetamino glucose after the hydrolysis of the alpha-N-acetamino glucose containing fluorophor, the speed of its hydrolysis substrate is directly proportional to the fluorescence intensity that discharges fluorescence molecule, so just can calculate the alpha-N-acetamino Polyglucosidase enzyme activity in sample to be tested.The mensuration reagent that adopts present method to prepare has convenient, fast and highly sensitive characteristics, easy to utilize.
Summary of the invention
The technical problem to be solved in the present invention is: provide a kind of fast, alpha-N-acetamino Polyglucosidase (α-N-acetylglucosaminidase accurately and reliably, EC 3.2.1.50) fluorescence detection of enzyme activity, the present invention simultaneously also is provided for the detection reagent of fluorescence spectrometry alpha-N-acetamino glucosidase activity, adopt the method and reagent to use on the fluorescence detection devices such as semi-automatic or full automatic fluorophotometer or microplate reader, and detection sensitivity is high, specificity is high, easy and simple to handle, thereby can obtain practical promoting the use of.
For the technical solution problem, technical scheme provided by the invention is as follows:
Alpha-N-acetamino Polyglucosidase activity determination method principle of the present invention is as follows:
R-alpha-N-acetamino glucose+H2O
the alpha-N-acetamino Polyglucosidase r-OH+alpha-N-acetamino glucose.
Be specially: the own non-blooming substrate R-alpha-N-acetamino glucose that the alpha-N-acetamino Polyglucosidase under given conditions can hydrolysis, discharge free fluorescence molecule R-OH, specifically excite with emission wavelength under the special fluorescence of R-OH can be detected, because the speed of alpha-N-acetamino Polyglucosidase hydrolysis substrate is directly proportional to the fluorescence intensity that discharges fluorescence molecule, therefore detect the R-OH fluorescent value under particular excitation wavelength and emission wavelength, just can calculate the enzyme activity of alpha-N-acetamino Polyglucosidase.
The general structural formula of alpha-N-acetamino glucose (α-N-acetylglucosaminide) is:
The general structural formula of substrate R-alpha-N-acetamino glucose provided by the invention is,
The general structural formula that specifically describes R-alpha-N-acetamino glucose is characterised in that: the aldehyde radical (CHO) on 1 C that is alpha-N-acetamino glucose at precursor structure is upper by special connection of the R-OH with possessing fluorescent characteristics generating R-alpha-N-acetamino glucose, the R-alpha-N-acetamino glucose generated has connected fluorophor R, but R-alpha-N-acetamino glucose itself does not possess fluorescent characteristics.When the alpha-N-acetamino Polyglucosidase can discharge again free R-OH molecule under given conditions during special hydrolysis R-alpha-N-acetamino glucose, specifically excite with emission wavelength under the special fluorescence of R-OH can be detected.
Wherein R-OH be in the physics and chemistry field general received any possess fluorescent characteristics and can with-molecule that CHO is reacted, it include but not limited to 4-methyl umbelliferone (4-methylumbelliferone) (A), hexadecyl-4 methyl umbelliferone (6-hexadecanoylamido-4-methylumbelliferone) (B), 3, tonka bean camphor (7-Hydroxy-4-(trifluoromethyl) coumarin) (D) for 4-dimethyl Umbelliferone (3,4-dimethylumbelliferone) (C), 7-hydroxyl-4-(trifluoromethyl).The molecule formed after R-OH is connected with alpha-N-acetamino glucose is followed successively by 4-methyl umbelliferone-alpha-N-acetamino glucose (4-methylumbelliferyl-N-acetyl-α-D-glucosamine), hexadecyl-4-methyl umbelliferone-alpha-N-acetamino glucose (6-hexadecanoylamido-4-methylumbelliferyl-N-acetyl-α-D-glucosamine), 3, 4-dimethyl Umbelliferone-alpha-N-acetamino glucose (3, 4-dimethylumbelliferyl-N-acetyl-α-D-glucosamine), the 4-(trifluoromethyl) tonka bean camphor-alpha-N-acetamino glucose (4-(trifluoromethyl) coumarin-N-acetyl-α-D-glucosamine).
R-OH can be connected and be formed R-alpha-N-acetamino glucose with the aldehyde radical (CHO) on 1 C on alpha-N-acetamino glucose.This synthetic substrate R-alpha-N-acetamino glucose can be discharged fluorescence molecule R-OH and alpha-N-acetamino glucose after the hydrolysis of alpha-N-acetamino Polyglucosidase under general received condition under the described condition of claims 6-11 of the present invention and on zymetology.
The glycyl group of substrate R-alpha-N-acetamino glucose of the present invention is to be connected on 2 C of alpha-N-acetamino glucose molecule.
Reagent with Fluorometric assay alpha-N-acetamino Polyglucosidase enzyme activity of the present invention, its main component comprises:
Damping fluid 20-1000mmol/L
PH scope 3-9.5
Stablizer 0.01%-10%
R-alpha-N-acetamino glucose 0.02-10mmol/L
Experiment shows, from the accuracy of measurement result and economy two aspects of preparation cost, considers, and no matter be single dose or two agent, the alpha-N-acetamino Polyglucosidase detection reagent of the present invention of following composition relation is comparatively desirable:
Damping fluid 50-200mmol/L
PH scope 4.0-5.5
Stablizer 0.1-0.5%
R-alpha-N-acetamino glucose 0.05-0.5mmol/L
Alpha-N-acetamino Polyglucosidase enzyme activity detection reagent of the present invention can be single dose, comprises damping fluid, stablizer, R-alpha-N-acetamino glucose.Reagent can be to make dry powder, uses after dissolving, or is made into liquid reagent, can directly use.
Also above-mentioned single dose reagent can be made into to following two agent reagent:
Reagent 1
Damping fluid, stablizer
Reagent 2
Damping fluid, R-alpha-N-acetamino glucose
Reagent can be to make dry powder, uses after dissolving, or is made into liquid reagent, can directly use.
Above-mentioned single dose reagent can also be made into to following two agent reagent:
Reagent 1
Damping fluid, stablizer
Reagent 2
R-alpha-N-acetamino glucose
Reagent can be to make dry powder, uses after dissolving, or is made into liquid reagent, can directly use.
Alpha-N-acetamino glucosidase activity detection reagent provided by the invention, described stablizer mainly comprises that the various tensio-active agents (detergent) of generally being accepted on physics and chemistry and using comprise following instance but are not limited to these examples:
Triton?x-100、Tween20、NP40、Brij-35
Buffer reagent in damping fluid described in alpha-N-acetamino Polyglucosidase enzymic activity detection reagent provided by the invention can or be stablized the reagent in 2-8.5 scope by the pH value of system buffering by generally accepted on physics and chemistry, comprises following instance but is not limited to these examples:
Sodium acetate/acetic acid: pH 2.6-5.8
Sodium formiate/formic acid: pH 2.0-5.0
Citric acid/sodium citrate: pH 3.0-6.6
Hydrophosphate/citric acid (salt): pH 2.2-8.0
Phosphoric acid salt: pH 4.9-8.2
Citric acid/sodium hydroxide/hydrochloric acid: pH 2.2-6.5
Tris-hydrochloric acid: pH 7.1-8.9
Alpha-N-acetamino Polyglucosidase method for detecting enzymatic activity provided by the invention and reagent, can be for detection of the enzyme activity of alpha-N-acetamino Polyglucosidase in the enzyme activity of the alpha-N-acetamino Polyglucosidase in sample to be tested, particularly human body body fluid or tissue or cell sample.Such as detecting alpha-N-acetamino Polyglucosidase vigor in the tissue such as cerebral tissue, hepatic tissue, muscular tissue, white corpuscle, inoblast or cell and blood plasma.
Alpha-N-acetamino glucosidase activity detection method provided by the invention and reagent, can be for detection of the enzyme activity of the alpha-N-acetamino Polyglucosidase in sample to be tested, thus can be widely applied to clinical detection or the diagnosis disease relevant to the enzyme activity of alpha-N-acetamino Polyglucosidase.
Embodiment
For comprehend and application the present invention, hereinafter with reference to embodiment, describe the present invention in detail, described embodiment is only intended as illustrative explanation the present invention, rather than intention limits the scope of the invention.Scope of the present invention is specifically limited by accompanying claims.
Embodiment
The alpha-N-acetamino Polyglucosidase detection reagent of the present embodiment is single reagent, comprises
Sodium phosphate dibasic/citrate buffer solution (pH4.5) 100mmol/L
Stablizer 0.1%
R-alpha-N-acetamino glucose 0.1mmol/L
Substrate R-alpha-N-acetamino glucose in reagent, R is 4-methyl umbelliferone.
The white corpuscle that the sample of tested alpha-N-acetamino Polyglucosidase is behaved, the total protein consumption is 0.05ug/ul, the volume ratio of albumen and reagent is 1/9, after adding sample and reagent, mixing makes it to react, reaction times is 24 hours, add the stop buffer termination reaction after reaction finishes, with fluorescence detection device, detect excitation wavelength 360nm(Ex=360nm), emission wavelength 460nm(Em=460nm) under fluorescent value.The reagent blank fluorescence value of reading average out to 30, normal people's white corpuscle fluorescent value of reading average out to 360, carry out the enzyme activity detection validation with the MPS-IIIB type and the MPS-I type patient that have made a definite diagnosis clinically.By the fluorescence standard curve, calculate the enzyme activity of alpha-N-acetamino Polyglucosidase, shown in seeing the following form.
13% left and right that the enzyme activity of MPS-IIIB type patient through more having made a definite diagnosis is normal individual, significant difference; CV<5%(n=3), illustrate that repeating effect is good, with the MPS-I type patient checking of having made a definite diagnosis clinically, result shows that the method for detection alpha-N-acetamino Polyglucosidase enzyme activity of the present invention and reagent detect and have specificity alpha-N-acetamino Polyglucosidase vigor.Experimental result shows, the method of detection alpha-N-acetamino Polyglucosidase enzyme activity of the present invention and reagent can effectively detect the alpha-N-acetamino Polyglucosidase enzyme activity in sample to be tested, can be applicable to clinical detection and the abnormal relevant disease of alpha-N-acetamino Polyglucosidase enzyme activity.
In a word, facts have proved, adopt method and the reagent of detection alpha-N-acetamino Polyglucosidase enzyme activity of the present invention fully can detect and draw required measurement result by general fluorescence detection device, and highly sensitive, tolerance range is good, specificity is high, is not subject to pollution and the interference of interior allogenic material, easy to utilize in the abnormal relevant disease of clinical detection and alpha-N-acetamino Polyglucosidase enzyme activity.
Claims (13)
1. an alpha-N-acetamino glucosidase activity measuring method is characterized in that its principle is as follows:
R-alpha-N-acetamino glucose+H2O
the alpha-N-acetamino Polyglucosidase 
r-OH+alpha-N-acetamino glucose.
2. be specially: the own non-blooming substrate R-alpha-N-acetamino glucose that the alpha-N-acetamino Polyglucosidase under given conditions can hydrolysis, discharge free fluorescence molecule R-OH, specifically excite with emission wavelength under the special fluorescence of R-OH can be detected, because the speed of alpha-N-acetamino Polyglucosidase hydrolysis substrate is directly proportional to the fluorescence intensity that discharges fluorescence molecule, therefore detect the R-OH fluorescent value under particular excitation wavelength and emission wavelength, just can calculate the enzyme activity of alpha-N-acetamino Polyglucosidase.
3. substrate R-alpha-N-acetamino glucose as claimed in claim 1 is characterized in that its universal architecture formula is:
Specifically describing this structural formula is characterised in that: at the aldehyde radical (CHO) of 1 C of precursor structure (alpha-N-acetamino glucose) and the special connection of R-OH that possesses fluorescent characteristics, generate R-alpha-N-acetamino glucose, the R-alpha-N-acetamino glucose generated has connected fluorophor R, but R-alpha-N-acetamino glucose itself does not possess fluorescent characteristics.
4. can discharge again under given conditions the free R-OH molecule that possesses fluorescent characteristics during special hydrolysis R-alpha-N-acetamino glucose when the alpha-N-acetamino Polyglucosidase.
5. as the R-OH that possesses fluorescent characteristics of claims 1 and 2 described substrate R-alpha-N-acetamino glucose and synthetic this substrate, it is characterized in that R-OH be in the physics and chemistry field general received any possess fluorescent characteristics and can with-molecule that CHO is reacted.
6. substrate R-alpha-N-acetamino glucose as described as claims 1-3, is characterized in that fluorescence molecule R-OH is connected and forms R-alpha-N-acetamino glucose with the aldehyde radical (CHO) on 1 C on alpha-N-acetamino glucose.
7. substrate R-alpha-N-acetamino glucose as described as claims 1-4, is characterized in that the glycyl group is to be on 2 C of alpha-N-acetamino glucose molecule.
8. an alpha-N-acetamino glucosidase activity detection reagent is characterized in that its main component comprises:
Damping fluid 20-1000mmol/L
PH scope 3-9.5
Stablizer 0.01%-10%
Substrate as described as claims 1-5 (R-alpha-N-acetamino glucose) 0.02-10mmol/L
According to claim 6, alpha-N-acetamino glucosidase activity detection reagent, is characterized in that: by damping fluid, stablizer, R-alpha-N-acetamino glucose, form single dose reagent.
9. alpha-N-acetamino glucosidase activity detection reagent according to claim 6, is characterized in that: by damping fluid, stablizer, R-alpha-N-acetamino glucose, form two agent reagent; Reagent 1, be comprised of damping fluid, stablizer; Reagent 2, be comprised of damping fluid, R-alpha-N-acetamino glucose.
10.9. alpha-N-acetamino glucosidase activity detection reagent, is characterized in that: by damping fluid, stablizer, R-alpha-N-acetamino glucose, form two agent reagent according to claim 6; Reagent 1, be comprised of damping fluid, stablizer; Reagent 2, be comprised of R-alpha-N-acetamino glucose.
11.10. according to the described alpha-N-acetamino glucosidase activity of claim 6-9 detection reagent, it is characterized in that: described stablizer mainly comprises by the various tensio-active agents of generally accepting on physics and chemistry and using.
12., as alpha-N-acetamino glucosidase activity detection reagent as described in claim 6-9, it is characterized in that: the buffer reagent in described damping fluid can or be stabilized in the reagent in 3-9.5 scope by the pH value of system buffering by generally accepted on physics and chemistry.
13. alpha-N-acetamino glucosidase activity detection reagent as described as claims 1-11, it is characterized in that, it can be for detection of the enzyme activity of the alpha-N-acetamino Polyglucosidase in sample to be tested, particularly the enzyme activity of alpha-N-acetamino Polyglucosidase in human body body fluid or tissue or cell sample, be applied to the disease that clinical detection or diagnosis are relevant to the enzyme activity of alpha-N-acetamino Polyglucosidase.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201210195780.0A CN103484525A (en) | 2012-06-14 | 2012-06-14 | Method, substrate and reagents for alpha-N-acetylglucosaminidase activity detection |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201210195780.0A CN103484525A (en) | 2012-06-14 | 2012-06-14 | Method, substrate and reagents for alpha-N-acetylglucosaminidase activity detection |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN103484525A true CN103484525A (en) | 2014-01-01 |
Family
ID=49825117
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201210195780.0A Pending CN103484525A (en) | 2012-06-14 | 2012-06-14 | Method, substrate and reagents for alpha-N-acetylglucosaminidase activity detection |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN103484525A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114835834A (en) * | 2022-05-16 | 2022-08-02 | 井冈山大学 | Modified cyclic oligomer and preparation method thereof, micro-nano fiber membrane and application thereof |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH01265897A (en) * | 1988-04-15 | 1989-10-23 | Nissan Gosei Kogyo Kk | Method for determining enzyme |
| CN1995975A (en) * | 2006-12-15 | 2007-07-11 | 苏州艾杰生物科技有限公司 | Method for detecting N-acetyl-beta-amino glucosaccharase activity and diagnosis kit therefor |
-
2012
- 2012-06-14 CN CN201210195780.0A patent/CN103484525A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH01265897A (en) * | 1988-04-15 | 1989-10-23 | Nissan Gosei Kogyo Kk | Method for determining enzyme |
| CN1995975A (en) * | 2006-12-15 | 2007-07-11 | 苏州艾杰生物科技有限公司 | Method for detecting N-acetyl-beta-amino glucosaccharase activity and diagnosis kit therefor |
Non-Patent Citations (1)
| Title |
|---|
| JANE MARSH, ET AL.: "4-methylumbelliferyl α-N-acetylglucosaminidase activity for diagnosis of Sanfilippo B disease", 《 CLINICAL GENETICS》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114835834A (en) * | 2022-05-16 | 2022-08-02 | 井冈山大学 | Modified cyclic oligomer and preparation method thereof, micro-nano fiber membrane and application thereof |
| CN114835834B (en) * | 2022-05-16 | 2023-03-10 | 井冈山大学 | Modified cyclic oligomer and preparation method thereof, micro-nano fiber membrane and application thereof |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| US7563591B2 (en) | Method for assaying the activity of lysosomal enzymes | |
| Wenger et al. | Synthetic substrate ß‐glucosidase activity in leukocytes: A reproducible method for the identification of patients and carriers of Gaucher's disease | |
| CN104267178B (en) | A kind of serum fucoside enzyme detection kit | |
| JP2009530310A (en) | Substrate and internal standard for mass spectrometry detection | |
| Stapleton et al. | Newborn screening for mucopolysaccharidoses: Measurement of glycosaminoglycans by LC-MS/MS | |
| CN107602643B (en) | Naphthalimide-based β -glucuronidase fluorescent probe and application thereof | |
| CN102864206A (en) | Anti-heparin interference leucine aminopeptidase measuring reagent | |
| Wolfe et al. | New substrates and enzyme assays for the detection of mucopolysaccharidosis III (Sanfilippo Syndrome) types A, B, C, and D by tandem mass spectrometry | |
| EP3031801A1 (en) | Specific fluorescent probe based on albumin pseudo-esterase hydrolysis reaction and use thereof | |
| CN111826417B (en) | N-acetyl-beta-D-glucosaminidase detection kit with good stability, preparation method and application | |
| CN109781719A (en) | A kind of kit detecting trypsase and its inhibitor based on platinum cluster | |
| CN102967571A (en) | Liquid single reagent for detecting alpha-L-fucosidase and preparation method thereof | |
| CN109824743B (en) | Fluorescent probe for detecting N-acetyl-beta-D-glucosaminidase and application thereof | |
| CN103512870A (en) | Enzyme activity detection method for mitochondrial respiratory chain complex IV and reagent | |
| CN103424384A (en) | Enzyme activity detection method for mitochondrial respiratory chain compound I and reagents | |
| CN103484525A (en) | Method, substrate and reagents for alpha-N-acetylglucosaminidase activity detection | |
| Volpi et al. | Plasmatic dermatan sulfate and chondroitin sulfate determination in mucopolysaccharidoses | |
| EP2700717B1 (en) | Method for the diagnosis of lysosomal storage diseases | |
| CN107655870A (en) | The detection method and detection kit of acidic hydrolysis enzymatic activity in a kind of lysosome | |
| CN103397076B (en) | The application in the biochemical reagents of negative value or null value is there is in chlorate in preparation elimination measurement result | |
| JP6360848B2 (en) | Rapid mass screening test for lysosomal disease 3 responsible enzyme | |
| CN103484526A (en) | Method, substrate and reagents for beta-hexosaminidase A activity detection | |
| JP2018102295A (en) | Simultaneous screening test for six congenital metabolic disorders | |
| CN103487414A (en) | Method, substrate and reagents for alpha-L-iduronidase activity detection | |
| US20060141551A1 (en) | Analytical method for detecting alkaline sphingomyelinase and kit for use in such method |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C53 | Correction of patent of invention or patent application | ||
| CB02 | Change of applicant information |
Address after: 102299, Changping District, Beijing, super Road, No. 6, building No. 4, layer 1001, 37 Applicant after: Outstanding Bioisystech Co., Ltd of BeiJing ZhongKe Address before: 102299, Changping District, Beijing, super Road, No. 6, building No. 4, layer 1001, 37 Applicant before: Beijing Hexin Feifan Biotechnology Co., Ltd. |
|
| COR | Change of bibliographic data |
Free format text: CORRECT: APPLICANT; FROM: BEIJING HEXIN FEIFAN BIOTECHNOLOGY CO., LTD. TO: BEIJING ZHONGKE FEIFAN BIOTECHNOLOGY CO., LTD. |
|
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20140101 |
|
| RJ01 | Rejection of invention patent application after publication |