CN103397076B - The application in the biochemical reagents of negative value or null value is there is in chlorate in preparation elimination measurement result - Google Patents
The application in the biochemical reagents of negative value or null value is there is in chlorate in preparation elimination measurement result Download PDFInfo
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Abstract
The invention discloses chlorate and eliminate in preparation the application that measurement result occurs in the biochemical reagents of negative value or null value, the novelty teabag of this chlorate is by adding chlorate in biochemical reagents, the chlorate added can provide suitable preservation and reaction environment for determinand, make determinand show due biologic activity, maintain the native conformation of determinand; And take part in the formation in test substance or toolenzyme active centre, ensure that reaction normally carry out obtain real detected result, eliminate the phenomenon measuring sample result and occur null value or negative value.
Description
Technical field
The invention belongs to field of biological detection, specifically, relate to chlorate and eliminate in preparation the application that measurement result occurs in the biochemical reagents of negative value or null value.
Background technology
High performance liquid chromatography and the detection of full-automatic biochemical detecting instrument is mainly contained at present for the instrument of biochemical indicator in detection bodies.Detecting with high performance liquid chromatograph device is generally acknowledged detection method, and detected result is accurate, but due to the method working method loaded down with trivial details, sample processing time is long and instrument costly need be used to support.Along with the development and perfection of modern zymetology and automatic clinical chemistry analyzer, increasing disease marker can be detected by design biochemical reagents.Automatic clinical chemistry analyzer is the instrument measuring certain specified chemical composition in body fluid according to photoelectric colorimetry principle.As to creatinine, TOTAL BILE ACID, adenosine deaminase detection etc.Biochemical reagents are utilize by a series of enzymatic reaction in process for preparation, make the chemical composition of specifying in determinand and color development system form hazardous substance, and this material obtains the value of determinand in sample to the interpretation developed the color by full automatic biochemical apparatus.Because automatic clinical chemistry analyzer measuring speed is fast, accuracy is high, consumption amount of reagent is little, be now used widely in situation of all-level hospitals, quarantine station, family planning services station and laboratory.And some determinand and toolenzyme have certain active biomacromolecule in body fluid, therefore need to provide suitable preservation and reaction environment, due biologic activity can be shown, thus the concentration of specifying chemical composition can accurately be measured in determinand.Present stage is to the Focal point and difficult point of the control of composition various in biochemical reagents and condition biochemical reagents research and development just.Therefore the accuracy that could improve the result that automatic clinical chemistry analyzer measures is continued to optimize to biochemical reagents.
Current biochemical reagents can be combined automatic clinical chemistry analyzer and be detected chemical compositions most in body, but clinically, some special patient is due to after own physiological reason or clinical application, when detecting the chemical composition in these patient's bodies, the chemical composition that usually result display is detected is shown as the illusion of zero or negative value, according to medical science general knowledge or more senior, measuring method is as high-efficient liquid phase technique more accurately, the measured value of the chemical composition that measurement result display is detected be on the occasion of.As the direct energy supply material that glucose is in human body, it maintains certain level in blood, ensures the normal functioning activity of each histoorgan of human body.Therefore the glucose of finite concentration level must be contained in humans and animals body, can not null value or negative value.Current biochemical reagents can not the concentration of kind chemical composition in Accurate Determining part sufferer in body.Therefore how to eliminate automatic clinical chemistry analyzer measurement result and occur that null value or negative value phenomenon are difficult points in the art.
Summary of the invention
Technical problem to be solved by this invention provides chlorate to eliminate in preparation the application that measurement result occurs in the biochemical reagents of negative value or null value for special sufferer, the novelty teabag of this chlorate is by adding chlorate in biochemical reagents, the chlorate added can provide suitable preservation and reaction environment for determinand, make determinand show due biologic activity, maintain the native conformation of determinand; And take part in the formation in test substance or toolenzyme active centre, ensure that reaction normally carry out obtain real detected result, eliminate the phenomenon measuring sample result and occur null value or negative value.
The present invention solves the problems of the technologies described above adopted technical scheme:
The application in the biochemical reagents of negative value or null value is there is in chlorate in preparation elimination measurement result, in biochemical reagents, add chlorate, in biochemical reagents preparation, constitute through adjustment pH, constant volume, the last toolenzyme that adds again the biochemical reagents product eliminated during biochemical reagents measure and occur null value or negative value.Due to special sufferer, the particular matter can secreted in body after self or drug administration can together be blended in body fluid, biochemical substances in determinand is surrounded by the particular matter that sufferer is secreted, when detecting with the body fluid of biochemical reagents to such sufferer not adding chlorate, enzyme in biochemical reagents can be secreted material intercept get up, the biological activity of enzyme can not get playing, thus have impact on the mensuration of biochemical reagents to the chemical substance of specifying in determinand, biochemical reagents there will be the phenomenon of negative value or null value together with the result of the chemical substance concentration of specifying in biochemical measurement Instrument measuring determinand.But the moment exists in vivo for the chemical substance of specifying in survey thing such as glucose or creatinine etc., and concentration can not be negative value or null value.After the chlorate added in biochemical reagents, the metal ion in chlorate can activate the enzyme in biochemical reagents, promotes carrying out smoothly of enzymatic reaction.Cl simultaneously in chlorate
-ion, as passivator, destroys the particular matter of sufferer self secretion, the protectiveness passive film that the particular matter removing sufferer secretion is formed; The structure of specifying biochemical substances can be maintained in determinand simultaneously, avoid the degraded of specifying biochemical substances in determinand.Make each component of biochemical reagents can carry out a series of reaction with all appointment chemical substances in determinand smoothly, form association thing, this association thing accurately can be detected by biochemical measurement instrument, can know the concentration of the biochemical substances of specifying in determinand according to detected result.The effect of metal ion in the chlorate added to enzyme has two aspects.One is the cofactor as enzyme, and two is as activator.K is had as activator
+, Na
+, Mg
2+, Zn
2+, Fe
3+and Ca
2+plasma.Chlorate can eliminate the phenomenon that negative value or null value appear in biochemical reagents measurement result.
In biochemical reagents, add chlorate, biochemical measurement instrument can be used to detect the concentration of designated substance in thing to be checked accurately, eliminate during biochemical reagents measure the illusion occurring null value or negative value.In biochemical reagents, add chlorate, chlorate can provide suitable preservation and reaction environment for determinand, makes determinand show due biologic activity, maintains the native conformation of determinand; And take part in the formation in test substance or toolenzyme active centre, ensure that reaction normally carry out obtain real detected result, solve the phenomenon measuring sample result and occur null value or negative value.Toolenzyme refers to the auxiliary enzymes or indicator enzyme that use in this reagent, as used in enzymic creatinine assay method reagent: sarcosine oxidase, creatine amidino groups lytic enzyme, peroxidase etc., are referred to as toolenzyme, and they are different in different reagent, differ and be decided to be same enzyme, because it has biological activity, therefore after in reagent, other preparation of raw material complete, finally can add.The biochemical reagents of biochemical reagents can be divided into single reagent biochemical reagents and double reagent biochemical reagents, are divided into R1 reagent biochemical reagents and R2 reagent biochemical reagents in double reagent biochemical reagents.When biochemical reagents are single reagent, in single reagent biochemical reagents, add chlorate, when biochemical reagents are double reagent, in any one reagent biochemical reagents of R1 or R2, add chlorate.Or add chlorate at R1 or R2 simultaneously.
Described chlorate is NaCl or MgCl
2or CaCl
2.NaCl, MgCl
2, CaCl
2the salts substances that three exists in human body or in animal body usually, select these three kinds of salts can preferably for determinand provides suitable preservation and reaction environment, determinand is made to show due biologic activity, maintain the natural structure of determinand, and take part in the formation in test substance or toolenzyme active centre, ensure that reaction normally carry out obtain real detected result, solve the phenomenon measuring sample result and occur null value or negative value.In three kinds of chlorates, NaCl is optimized choice, NaCl is usually used in life, easy acquisition, as for the preparation of physiological saline, NaCl can maintain very well and adapt to the environment in body in body, in like manner NaCl also can be good at more preferably to provide suitable preservation and reaction environment by serum determinand, makes determinand show due biologic activity, maintains the native conformation of determinand; And take part in the formation in test substance or toolenzyme active centre, ensure that reaction normally carry out obtain real detected result, eliminate the phenomenon measuring sample result and occur null value or negative value.
The concentration of described chlorate is 1-50g/L.When the concentration of chlorate can well eliminate the phenomenon measuring sample result and occur null value or negative value in the scope of 1-50g/L.When the concentration of chlorate is being less than 1g/L, the too small deficiency of concentration thinks that determinand provides suitable preservation and reaction environment, determinand can not show due biologic activity, can only the life of recuperation section active, although can eliminate the phenomenon measuring sample result and occur null value or negative value, institute's measurement result can not reflect in determinand the concentration of specifying biological really.When the concentration of chlorate is when being greater than 50g/L, the preservation that too high concentration can be carried determinand on the contrary and reaction environment cause disadvantageous effect, thus have slight restraining effect to the due biologic activity of determinand.Although can eliminate the phenomenon measuring sample result and occur null value or negative value, institute's measurement result can not reflect in determinand the concentration of specifying biological really.
Described biochemical reagents comprise the biochemical reagents that the biochemical reagents of creatinine assay, the biochemical reagents of tolal bile acid determination and adenosine deaminase measure.The biochemical reagents of creatinine assay are the concentration in order to detect creatinine in determinand, the biochemical reagents of tolal bile acid determination are the concentration in order to detect TOTAL BILE ACID in determinand, and the biochemical reagents that adenosine deaminase measures are the concentration in order to detect adenosine deaminase in determinand.In addition, as needed other biochemical indicators in human body or in animal body, the biochemical reagents of corresponding different biochemical measurement can be configured.In different biochemical reagents, add chlorate, all can eliminate the phenomenon that negative value or null value appear in biochemical reagents measurement result.
Described NaCl concentration of adding at creatinine assay biochemical reagents is 1 ~ 10g/L.According to the physico-chemical property of creatinine self, the NaCl concentration of adding in the biochemical reagents of creatinine assay is 1 ~ 10g/L, the NaCl of this concentration better for determinand provides suitable preservation and reaction environment, can make determinand show due biologic activity, maintains the native conformation of determinand; And take part in the formation in test substance or toolenzyme active centre, ensure that reaction normally carry out obtain real creatinine detected result, eliminate the phenomenon measuring sample result and occur null value or negative value.
Described NaCl concentration of adding at tolal bile acid determination biochemical reagents is 1 ~ 15g/L.According to the physico-chemical property of TOTAL BILE ACID, the NaCl concentration of adding in tolal bile acid determination biochemical reagents is 1 ~ 15g/L, the NaCl of this concentration can better for determinand provides suitable preservation and reaction environment, make determinand show due biologic activity, maintain the native conformation of determinand; And take part in the formation in test substance or toolenzyme active centre, ensure that reaction normally carry out obtain real TOTAL BILE ACID detected result, solve the phenomenon measuring sample result and occur null value or negative value.
Described NaCl concentration of adding at adenosine deaminase mensuration biochemical reagents is 10 ~ 50g/L.According to the physico-chemical property of adenosine deaminase, measuring the NaCl concentration of adding in biochemical reagents at adenosine deaminase is 10 ~ 50g/L, the NaCl of this concentration can better for determinand provides suitable preservation and reaction environment, make determinand show due biologic activity, maintain the native conformation of determinand; And take part in the formation in test substance or toolenzyme active centre, ensure that reaction normally carry out obtain real adenosine deaminase detected result, solve the phenomenon measuring sample result and occur null value or negative value.
Described NaCl concentration of adding at creatinine assay biochemical reagents is 3g/L.The concentration that middle interpolation NaCl joined by creatinine assay biochemical reagents be 3g/L is optimized choice, when the concentration of adding NaCl is 3g/L, better for determinand provides suitable preservation and reaction environment, determinand can be made to show due biologic activity, maintains the native conformation of determinand; And take part in the formation in test substance or toolenzyme active centre, ensure that reaction normally carry out obtain real creatinine detected result, solve the phenomenon measuring sample result and occur null value or negative value.
Described NaCl concentration of adding at tolal bile acid determination biochemical reagents is 9g/L.The concentration of adding NaCl in tolal bile acid determination biochemical reagents is 9g/L is optimized choice, when add NaCl concentration for 9g/L can better for determinand provides suitable preservation and reaction environment, make determinand show due biologic activity, maintain the native conformation of determinand; And take part in the formation in test substance or toolenzyme active centre, ensure that the Concentration Testing result normally carrying out obtaining true TOTAL BILE ACID of reaction, solve the phenomenon measuring sample result and occur null value or negative value.
Described NaCl concentration of adding at adenosine deaminase mensuration biochemical reagents is 30g/L.The NaCl concentration of adding in adenosine deaminase mensuration biochemical reagents is 30g/L is a selection optimized, when the NaCl concentration of adding is 30g/L, can better for determinand provides suitable preservation and reaction environment, make determinand show due biologic activity, maintain the native conformation of determinand; And take part in the formation in test substance or toolenzyme active centre, ensure that reaction normally carry out obtain real adenosine deaminase Concentration Testing result, solve the phenomenon measuring sample result and occur null value or negative value
In sum, the invention has the beneficial effects as follows:
Chlorate is provided to eliminate in preparation the application that measurement result occurs in the biochemical reagents of negative value or null value for special sufferer, the novelty teabag of this chlorate is by adding chlorate in biochemical reagents, the chlorate added can provide suitable preservation and reaction environment for determinand, make determinand show due biologic activity, maintain the native conformation of determinand; And take part in the formation in test substance or toolenzyme active centre, ensure that reaction normally carry out obtain real detected result, eliminate the phenomenon measuring sample result and occur null value or negative value.
Embodiment
Below in conjunction with embodiment, the present invention is described in further detail, but embodiments of the present invention are not limited only to this.
Embodiment 1:
The application in the biochemical reagents of negative value or null value is there is in chlorate in preparation elimination measurement result.In biochemical reagents, add chlorate, chlorate can provide suitable preservation and reaction environment for determinand, makes determinand show due biologic activity, maintains the native conformation of determinand; And take part in the formation in test substance or toolenzyme active centre, ensure that reaction normally carry out obtain real detected result, eliminate the phenomenon measuring sample result and occur null value or negative value.
Embodiment 2:
There is the application in the biochemical reagents of negative value or null value in chlorate, in the layoutprocedure of biochemical reagents, adds chlorate in preparation elimination measurement result.Described chlorate is NaCl or MgCl
2or CaCl
2.NaCl, MgCl
2, CaCl
2the concentration of three is 1-50g/L.Biochemical reagents comprise the biochemical reagents configuration that the biochemical reagents of creatinine assay, the biochemical reagents of tolal bile acid determination and adenosine deaminase measure.NaCl, MgCl
2, CaCl
2the salts substances that three exists in human body or in animal body usually, select these three kinds of salts can well provide suitable preservation and reaction environment for determinand, determinand is made to show due biologic activity, maintain the natural structure of determinand, and take part in the formation in test substance or toolenzyme active centre, ensure that reaction normally carry out obtain real detected result, solve the phenomenon measuring sample result and occur null value or negative value.As NaCl, MgCl
2, CaCl
2the concentration of three can well eliminate the phenomenon measuring sample result and occur null value or negative value in the scope of 1-50g/L.As NaCl, MgCl
2, CaCl
2the concentration of three is being less than 1g/L, and the too small deficiency of concentration thinks that determinand provides suitable preservation and reaction environment, makes determinand can not show due biologic activity.As NaCl, MgCl
2, CaCl
2the concentration of three is when being greater than 50g/L, and the preservation that too high concentration can be carried determinand on the contrary and reaction environment cause slight disadvantageous effect, thus suppresses the due biologic activity of part determinand.
Embodiment 3:
Measure the concentration of sufferer creatinine in serum with the biochemical reagents of creatinine assay, these biochemical reagents are double reagent, and double reagent is respectively R1 and R2, add NaCl in the R1 in double reagent, and the concentration of NaCl is 1-10g/L, and detailed process is:
The process of configuration R1 reagent biochemical reagents is, about 700ml purified water (purified water quality meets the requirement of external diagnosis reagent water) is added in the clean beaker of 1000ml, then HEPES and 2-[4-(2-hydroxyethyl)-1-piperazine] the ethyl sulfonic acid 11.92g of 50mmol/L is taken, add stirring and dissolving in beaker, then tensio-active agent TX-100 and the Triton-100 of 1ml is added, stirring and dissolving, add TOOS and N-ethyl-N-(2-hydroxyl-3-sulfopropyl again)-3-monomethylaniline 0.99g, add 1-10gNaCl, stirring and dissolving mixes, with the NaOH initial adjustment reagent pH to 7.6 of 20%, be settled to 1000ml, add sarcosine oxidase 10ku again, creatine amidino groups lytic enzyme 50ku,
The process of configuration R2 reagent biochemical reagents is, about 700ml purified water is added in the clean beaker of 1000ml, purified water quality symbol need close the requirement of external diagnosis reagent water, then 2-[4-(2-hydroxyethyl)-1-piperazine] the ethyl sulfonic acid 11.92g of 50mmol/L is taken, add stirring and dissolving in beaker, then pH to 7.6 ± 0.1 is regulated to form damping fluid with the NaOH of 20%, add 4-AAP and 4-AA 0.61g stirring and dissolving again, be settled to 1000ml, finally add peroxidase 8ku, add creatinine amidohydrolase 250ku;
Carry out analysis with full-automatic or semi-automatic biochemical analyzer to measure, location parameter is as follows:
Method: end-point method sample reagent is than being sample: R1:R2=5:225:75
Measure wavelength: 546nm temperature of reaction 37 DEG C of reaction times: 10min.
Embodiment 4:
The concentration of TOTAL BILE ACID in sufferer serum is detected with the biochemical reagents of tolal bile acid determination.These biochemical reagents are double reagent, and double reagent is respectively R1 and R2, and add concentration in R1 and R2 in double reagent is 1 ~ 15g/LNaCl simultaneously, and detailed process is:
The process of configuration R1 reagent biochemical reagents is, about 700ml purified water is added in the clean beaker of 1000ml, purified water quality need meet the requirement of external diagnosis reagent water, then CAPS and N-cyclohexyl-Homotaurine and the 12.97g of 50mmol/L is taken, add stirring and dissolving in beaker, then pH to 5.05 ± 0.05 is adjusted to form damping fluid with HCL, add tensio-active agent TX-100 and Triton-1001ml stirring and dissolving again, add NaCl9g again, and the Thio-NAD of 1g and Thio-NAD, stirring and dissolving mixes, be settled to 1000ml,
The layoutprocedure of configuration R2 reagent biochemical reagents, about 700ml purified water is added in the clean beaker of 1000ml, purified water quality need meet the requirement of external diagnosis reagent water, then Tris and the Tutofusin tris 12.11g of 100mmol/L is taken, add stirring and dissolving in beaker, then 60mmol/LTAPS and N-tri-(methylol) methyl-3-aminopropanesulfonicacid acid 14.60g is taken, stirring and evenly mixing, add 1ml tensio-active agent TX-100 and Triton-100 again, add NaCl9g, add bovine serum albumin 5g, stirring and dissolving mixes, after be settled to 1000ml.Add 3 α-HSD i.e. 3-α-steroid dehydrogenase 6ku and β-NADH i.e. reduced form acid amides adenine dinucleotide 6g again;
Carry out analysis with full-automatic or semi-automatic biochemical analyzer to measure, location parameter is as follows:
Method: the volume ratio of rate method sample reagent is, sample: R1:R2=:5:225:75
Measure wavelength: 405nm temperature of reaction 37 DEG C of reaction times: 10min.
Embodiment 5:
The biochemical reagents measured with adenosine deaminase measure the concentration of adenosine deaminase.These biochemical reagents are double reagent, and double reagent is respectively R1 and R2, and add concentration in the process for preparation of the R2 in double reagent is 10 ~ 50g/LNaCl simultaneously, and detailed process is:
Configuration R1 reagent biochemical reagents layoutprocedure, about 700ml purified water is added in the clean beaker of 1000ml, purified water quality need meet the requirement of external diagnosis reagent water, then K2HPO4 and the dipotassium hydrogen phosphate 11.41g of 50mmol/L is taken, add stirring and dissolving in beaker, then add NaH2PO4 and the SODIUM PHOSPHATE, MONOBASIC 3.60g formation damping fluid of 30mmol/L, then add EDTA-2Na and disodium ethylene diamine tetraacetate 1.86g, mix and dissolve mixing, be settled to 1000ml.Add POD and peroxidase 5ku again, add XOD and XOD 5ku, add PNP and purine nucleoside phosphorylase 5ku and ASOM and Vitamin C oxidase 10ku;
The layoutprocedure of configuration R2 reagent biochemical reagents is, about 700ml purified water is added in the clean beaker of 1000ml, purified water quality need meet the requirement of external diagnosis reagent water, then MES and the MES 9.76g of 50mmol/L is taken, add stirring and dissolving in beaker, NaOH with 20% adjusts PH to 6.5 ± 0.05 to form damping fluid, then adenosine 5.35g is taken, stirring and evenly mixing, add NaCl30g again, and TOOS and N-ethyl-N-(2-hydroxyl-3-sulfopropyl)-3-methylbenzene amine salt 0.99g, stirring and dissolving mix, after be settled to 1000ml;
Carry out analysis with full-automatic or semi-automatic biochemical analyzer to measure, location parameter is as follows:
Method: the volume ratio of rate method sample reagent is, sample: R1:R2=5:225:75
Measure wavelength: 546nm temperature of reaction 37 DEG C of reaction times: 10min.
Embodiment 6:
Measure the glucose concn in sufferer serum with the biochemical reagents of glucose assays, these biochemical reagents are single reagent, and in reagent, add concentration is 1 ~ 15g/LNaCl, and detailed process is:
Configuration R reagent biochemical reagents, about 700ml purified water is added in the clean beaker of 1000ml, purified water quality need meet the requirement of external diagnosis reagent water, then 50mmol/LMES and MES 9.76g is taken, add stirring and dissolving in beaker, NaOH with 20% adjusts pH to 6.5 ± 0.1 to form damping fluid, add tensio-active agent TX-100 and the Triton-100 of 1ml, add 4-AAP and 4-AA 0.11g, and phenol 0.19g, 8gNaCl, stirring and evenly mixing, is settled to 1000ml.Add GOD and glucose oxidase 28ku again, add POD and peroxidase 5ku;
Carry out analysis with full-automatic or semi-automatic biochemical analyzer to measure, location parameter is as follows:
Method: the volume ratio of end-point method sample reagent is, sample: R1=3:300
Measure wavelength: 505nm temperature of reaction 37 DEG C of reaction times: 10min.
Through detected result, the beneficial effect that biochemical reagents of the present invention play is described below:
Detect the concentration of creatinine in serum sample to be tested in 10 different sufferers, 10 kinds of different serum testing samples, often kind of testing sample 3 parts, often kind of testing sample detects the concentration of creatinine in testing sample respectively by 3 kinds of modes, 3 kinds of modes are respectively: sophisticated method HPLC and high performance liquid chromatography detect the creatine concentration in sample, the creatine concentration be added with in the creatinine assay biochemical reagents associating Biochemical Analyzer detection sample of NaCl, and detect the creatine concentration in sample with the creatinine assay biochemical reagents associating Biochemical Analyzer not adding NaCl.The biochemical reagents being added with NaCl are in the biochemical reagents not adding NaCl, add the NaCl that concentration is 1 ~ 50g/L.3 kinds of modes measure in sufferer blood that creatine concentration Comparative result data are in table 1, and in table, data unit is a μm ol/L:
In human muscular tissue, creatine forms creatinine lentamente mainly through irreversible non-enzymatic dehydration reaction, then is discharged in blood, then by glomerular filtration with homaluria.According to medical science general knowledge, in human body or animal body in blood plasma containing certain density creatinine, can not be negative value or null value.As shown in table 1 is that the concentration results of creatinine that the biochemical reagents of the NaCl of 1 ~ 50g/L measure in 10 serum samples of sufferer can be found out with being added with concentration, the result that the result of gained and high performance liquid chromatography measure is detected very close with the biochemical reagents being added with NaCl in the present invention, detected result accuracy is high, can replace high performance liquid chromatography.Detecting the creatine concentration in sample with the biochemical reagents not adding NaCl, there is negative value or null value in the result that acquisition detects in institute.Show to add NaCl in biochemical reagents and can eliminate negative value or null value appear in biochemical reagents phenomenon in detected result.
All conditions is as the testing process of above-mentioned creatine concentration, the creatinine assay biochemical reagents being added with NaCl are in the biochemical reagents not adding NaCl, add the NaCl that concentration is 0.5g/L, 3 kinds of modes measure in sufferer blood that creatine concentration Comparative result data are as follows in table 2, and in table, data unit is a μm ol/L::
As shown in table 2 is that the concentration results of creatinine that the biochemical reagents of the NaCl of 0.5g/L measure in 10 serum samples of sufferer can be found out with being added with concentration, detecting the creatine concentration in sample with the biochemical reagents not adding NaCl, there is negative value or null value in the result that acquisition detects in institute.And detect the concentration results of gained with the biochemical reagents being added with NaCl in the present invention and be all greater than zero, show to add NaCl in biochemical reagents and can eliminate negative value or null value appear in biochemical reagents phenomenon in detected result.But differ larger at the creatine concentration of biochemical reagents in detection sample of the NaCl being added with concentration 0.5g/L with the result that high performance liquid chromatography measures.When the concentration of chlorate is being less than 1g/L, the too small deficiency of concentration thinks that determinand provides suitable preservation and reaction environment, determinand can not show due biologic activity, can only the life of recuperation section active, although can eliminate the phenomenon measuring sample result and occur null value or negative value, institute's measurement result can not reflect in determinand the concentration of specifying biological really.
All conditions is as the testing process of above-mentioned creatine concentration, the creatinine assay biochemical reagents being added with NaCl are in the biochemical reagents not adding NaCl, add the NaCl that concentration is 80g/L, 3 kinds of modes measure in sufferer blood that creatine concentration Comparative result data are as follows in table 3, and in table, data unit is a μm ol/L::
As shown in table 3 is that the concentration results of creatinine that the biochemical reagents of the NaCl of 80g/L measure in 10 serum samples of sufferer can be found out with being added with concentration, detecting the creatine concentration in sample with the biochemical reagents not adding NaCl, there is negative value or null value in the result that acquisition detects in institute.And detect the concentration results of gained with the biochemical reagents being added with NaCl in the present invention and be all greater than zero, show to add NaCl in biochemical reagents and can eliminate negative value or null value appear in biochemical reagents phenomenon in detected result.But differ larger at the creatine concentration of biochemical reagents in detection sample of the NaCl being added with concentration 80g/L with the result that high performance liquid chromatography measures.When the concentration of chlorate is when being greater than 50g/L, the preservation that too high concentration can be carried determinand on the contrary and reaction environment cause disadvantageous effect, thus have slight restraining effect to the due biologic activity of determinand.Although can eliminate the phenomenon measuring sample result and occur null value or negative value, institute's measurement result can not reflect in determinand the concentration of specifying biological really.
Detect the concentration of TOTAL BILE ACID in serum sample to be tested in 10 different sufferers, 10 kinds of different serum testing samples, often kind of testing sample 3 parts, often kind of testing sample detects the concentration of TOTAL BILE ACID in testing sample respectively by 3 kinds of modes, 3 kinds of modes are respectively: sophisticated method HPLC and high performance liquid chromatography detect the concentration of total bile acid in sample, the concentration of total bile acid used in agents Biochemical Analyzer of the present invention detection sample, and detect the concentration of total bile acid in sample with general reagent associating Biochemical Analyzer.With reagent of the present invention be add in the layoutprocedure of general reagent add concentration be the NaCl of 1 ~ 15g/L.3 kinds of modes measure in sufferer blood that concentration of total bile acid Comparative result data are in table 4, and in table, data unit is a μm ol/L:
In human body, TOTAL BILE ACID is all being discharged in blood always slowly.According to medical science general knowledge, in human body or animal body in blood plasma containing certain density TOTAL BILE ACID, can not be negative value or null value.As shown in table 4 is that the concentration results of TOTAL BILE ACID that the biochemical reagents of the NaCl of 1 ~ 15g/L measure in 10 serum samples of sufferer can be found out with being added with concentration, the result that the result of gained and high performance liquid chromatography measure is detected very close with the biochemical reagents being added with NaCl in the present invention, detected result accuracy is high, can replace high performance liquid chromatography.Detecting the concentration of total bile acid in sample with the biochemical reagents not adding NaCl, there is negative value or null value in the result that acquisition detects in institute.Show to add NaCl in biochemical reagents and can eliminate negative value or null value appear in biochemical reagents phenomenon in detected result.
Detect the concentration of adenosine deaminase in serum sample to be tested in 10 different sufferers, 10 kinds of different serum testing samples, often kind of testing sample 3 parts, often kind of testing sample detects the concentration of adenosine deaminase in testing sample respectively by 3 kinds of modes, 3 kinds of modes are respectively: sophisticated method HPLC and high performance liquid chromatography detect the adenosine deaminase concentration in sample, the adenosine deaminase concentration used in agents Biochemical Analyzer of the present invention detection sample, and detect the adenosine deaminase concentration in sample with general reagent associating Biochemical Analyzer.With reagent of the present invention be add in the layoutprocedure of general reagent add concentration be the NaCl of 10 ~ 50g/L.3 kinds of modes measure in sufferer blood that adenosine deaminase concentration results correlation data is in table 5, and in table, data unit is a μm ol/L:
Adenosine deaminase is distributed widely in during human body respectively organizes, according to medical science general knowledge, in human body or animal body in blood plasma containing certain density adenosine deaminase, can not be negative value or null value.As shown in table 5 is that the concentration results of adenosine deaminase that the biochemical reagents of the NaCl of 10 ~ 50g/L measure in 10 serum samples of sufferer can be found out with being added with concentration, the result that the result of gained and high performance liquid chromatography measure is detected very close with the biochemical reagents being added with NaCl in the present invention, detected result accuracy is high, can replace high performance liquid chromatography.Detecting the adenosine deaminase concentration in sample with the biochemical reagents not adding NaCl, there is negative value or null value in the result that acquisition detects in institute.Show to add NaCl in biochemical reagents and can eliminate negative value or null value appear in biochemical reagents phenomenon in detected result.
Claims (5)
1. there is the application in the biochemical reagents of negative value or null value in chlorate in preparation elimination measurement result, it is characterized in that, described biochemical reagents comprise the biochemical reagents that the biochemical reagents of creatinine assay, the biochemical reagents of tolal bile acid determination and adenosine deaminase measure; When described biochemical reagents are the double reagent of creatinine assay, double reagent is respectively R1 and R2, adds the NaCl that concentration is 1-50g/L in the R1 of double reagent; When described biochemical reagents are the double reagent of tolal bile acid determination, double reagent is respectively R1 and R2, all adds the NaCl that concentration is 1-15g/L in R1 and R2 of double reagent; When described biochemical reagents are the double reagent of adenosine deaminase mensuration, double reagent is respectively R1 and R2, adds the NaCl that concentration is 10-50g/L in the R2 of double reagent;
Wherein, the layoutprocedure measuring the R1 reagent biochemical reagents of creatinine is: in 1000ml beaker, add 700ml purified water, then 2-[4-(2-hydroxyethyl)-1-piperazine] the ethyl sulfonic acid 11.92g of 50mmol/L is taken, add stirring and dissolving in beaker, then the tensio-active agent TX-100 of 1ml is added, stirring and dissolving, add N-ethyl-N-(2-hydroxyl-3-sulfopropyl again)-3-monomethylaniline 0.99g, add 1-50gNaCl, stirring and dissolving mixes, with the NaOH initial adjustment reagent pH to 7.6 of 20%, be settled to 1000ml, add sarcosine oxidase 10ku again, creatine amidino groups lytic enzyme 50ku,
The layoutprocedure measuring the R2 reagent biochemical reagents of creatinine is: in 1000ml beaker, add 700ml purified water, then 2-[4-(2-hydroxyethyl)-1-piperazine] the ethyl sulfonic acid 11.92g of 50mmol/L is taken, add stirring and dissolving in beaker, then pH to 7.6 ± 0.1 is regulated to form damping fluid with the NaOH of 20%, add 4-AA 0.61g stirring and dissolving again, be settled to 1000ml, finally add peroxidase 8ku, add creatinine amidohydrolase 250ku;
The layoutprocedure measuring the R1 reagent biochemical reagents of TOTAL BILE ACID is: in 1000ml beaker, add 700ml purified water, then the CAPS12.97g of 50mmol/L is taken, add stirring and dissolving in beaker, then pH to 5.05 ± 0.05 is adjusted to form damping fluid with HCL, add tensio-active agent TX-1001ml stirring and dissolving again, then add NaCl1-15g, and the Thio-NAD of 1g, stirring and dissolving mixes, and is settled to 1000ml;
The layoutprocedure measuring the R2 reagent biochemical reagents of TOTAL BILE ACID is: in 1000ml beaker, add 700ml purified water, then the Tutofusin tris 12.11g of 100mmol/L is taken, add stirring and dissolving in beaker, then take 60mmol/LTAPS14.60g, stirring and evenly mixing, add 1ml tensio-active agent TX-100 again, add NaCl1-15g, add bovine serum albumin 5g, stirring and dissolving mixes, after be settled to 1000ml, then add 3 α-HSD6ku and β-NADH6g;
The layoutprocedure measuring the R1 reagent biochemical reagents of adenosine deaminase is: in 1000ml beaker, add 700ml purified water, then the dipotassium hydrogen phosphate 11.41g of 50mmol/L is taken, add stirring and dissolving in beaker, the SODIUM PHOSPHATE, MONOBASIC 3.60g adding 30mmol/L again forms damping fluid, then disodium ethylene diamine tetraacetate 1.86g is added, mix and dissolve mixing, be settled to 1000ml, add peroxidase 5ku again, add XOD 5ku, add purine nucleoside phosphorylase 5ku and Vitamin C oxidase 10ku;
The layoutprocedure measuring the R2 reagent biochemical reagents of adenosine deaminase is: in 1000ml beaker, add 700ml purified water, then the MES 9.76g of 50mmol/L is taken, add stirring and dissolving in beaker, NaOH with 20% adjusts PH to 6.5 ± 0.05 to form damping fluid, then takes adenosine 5.35g, stirring and evenly mixing, add NaCl10-50g again, and N-ethyl-N-(2-hydroxyl-3-sulfopropyl)-3-methylbenzene amine salt 0.99g, stirring and dissolving mix, after be settled to 1000ml.
2. application according to claim 1, is characterized in that, described NaCl concentration of adding at creatinine assay biochemical reagents is 1-10g/L.
3. application according to claim 2, is characterized in that, described NaCl concentration of adding at creatinine assay biochemical reagents is 3g/L.
4. application according to claim 1, is characterized in that, described NaCl concentration of adding in tolal bile acid determination biochemical reagents R1 and R2 is 9g/L.
5. application according to claim 1, is characterized in that, described NaCl concentration of adding at adenosine deaminase mensuration biochemical reagents is 30g/L.
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| CN108007922B (en) * | 2017-12-21 | 2018-11-13 | 广州市进德生物科技有限公司 | A kind of kit detecting glucose using luminol chemiluminescence analysis |
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| Technicon RA-XT型生化分析仪测定血清总胆汁酸及其临床应用;王跃国等;《陕西医学检验》;19950831;第10卷(第3期);全文 * |
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