CN103480029B - Haemostatic dressing and production method thereof - Google Patents
Haemostatic dressing and production method thereof Download PDFInfo
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- CN103480029B CN103480029B CN201310315115.5A CN201310315115A CN103480029B CN 103480029 B CN103480029 B CN 103480029B CN 201310315115 A CN201310315115 A CN 201310315115A CN 103480029 B CN103480029 B CN 103480029B
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- chitosan fiber
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Abstract
The invention provides a haemostatic dressing and a production method thereof. The haemostatic dressing comprises 25 to 50 wt% of a chitosan fiber having undergone acid treatment and 50 to 75 wt% of a chitosan fiber having undergone cross-linking treatment. The haemostatic dressing provided by the invention has an excellent haemostatic effect and can maintain high stress intensity in use.
Description
Technical field
The present invention about a kind of bleeding-stopping dressing and manufacture method thereof, and particularly about a kind of bleeding-stopping dressing and the manufacture method thereof that comprise chitosan fiber.
Background technology
Chitosan (Chitosan) is by the macromolecule of chitin (Chitin) gained after deacetylation; chitin is then the natural polymer extracted from biology, is mainly present in Crustacean (shrimp, Eriocheir sinensis), insecticide shell or the cell wall of Mycophyta.Chitin is with the straight-chain macromolecule polysaccharide polymer of β-Isosorbide-5-Nitrae bond by glucamine and N-Acetyl-D-glucosamine.Chitosan because having good biocompatibility, biological activity, no cytotoxicity and Biodegradable with iuntercellular, therefore can be applicable to biomaterial and medical material.Chitosan, in an acidic solution with positive electricity, therefore can attract platelet aggregation, and then reaches the effect of quick-acting haemostatic powder.
At present, extensive in clinical middle application with the dressing of chitosan adhesive-bonded fabric composition, but chitosan dressing has the not good shortcoming of stress, after contact blood, can form gel fast and rupture.In correlative technology field, there is the method that some increase bleeding-stopping dressing stress intensity.Such as, US Patent No. 7,981,872 exposure utilize rayon (rayon) fiber to meet water-insoluble characteristic to maintain the toughness of chitosan dressing, and then improve the not good problem of chitosan dressing stress intensity.U.S. Patent Publication case US2011/0236433 discloses the adhesive-bonded fabric dressing becoming to contain two kinds of fibrous materials in conjunction with chitosan fiber and cellulose (cellulose) fiber combinations, also be utilize the water-fast high-strength characteristic of cellulose fibre itself, maintain the tensile strength of dressing when patient wound uses.But, meet water-insoluble material can maintain dressing stress though add another, also can reduce the ratio of chitosan fiber simultaneously, and then cause dressing hemostatic capability to reduce.Therefore, how to improve chitosan proportion of fibers, maintain the stress intensity after dressing chance water simultaneously, be still the problem that need overcome.
Summary of the invention
Therefore, an object of the present invention is the bleeding-stopping dressing and the manufacture method thereof that provide a kind of novelty, to make bleeding-stopping dressing have haemostatic effect and stress intensity concurrently, to improve shortcoming of the prior art.
The invention provides a kind of bleeding-stopping dressing, comprising: percentage by weight be 25% to 50% the chitosan fiber through acidification and percentage by weight be 50% to 75% the chitosan fiber through crosslinking Treatment.
According to an embodiment of the present invention, the dry tensile strength of this bleeding-stopping dressing is 1000 gram force to 2600 gram forces, and wet tensile strength is 200 gram force to 600 gram forces.
According to an embodiment of the present invention, the amido weight content of this bleeding-stopping dressing is 50% to 90%.
Another object of the present invention provides a kind of manufacture method of bleeding-stopping dressing, comprising:
A chitosan fiber is dissolved in acidic aqueous by (), form acidify chitosan fiber solution;
B acidify chitosan solution carries out dewatering and drying and processing by (), form acidify chitosan fiber;
C chitosan fiber and cross-linking agent are dissolved in organic solvent by (), form crosslinked chitosan fiber solution after polyreaction;
D crosslinked chitosan fiber solution carries out dewatering and drying and processing by (), form crosslinked chitosan fiber; And
E acidify chitosan fiber and crosslinked chitosan fiber are made chitosan adhesive-bonded fabric with tailor's cushion method by ();
Wherein, chitosan adhesive-bonded fabric comprise percentage by weight be 25% to 50% acidify chitosan fiber and percentage by weight be 50% to 75% crosslinked chitosan fiber.
According to an embodiment of the present invention, the Deacetylation degree of this chitosan fiber is 80% to 99%.
According to an embodiment of the present invention, this acidic aqueous comprises organic solvent and acid solution; Wherein, this acid solution is selected from the group that phosphoric acid, citric acid, malic acid, succinic acid, acetic acid, hydrochloric acid and lactic acid form.
According to an embodiment of the present invention, the pH value of this acidic aqueous is 2 to 6.
According to an embodiment of the present invention, this organic solvent is selected from the group that methanol, ethanol, acetone and isopropyl alcohol form.
According to an embodiment of the present invention, this cross-linking agent is selected from the group that formaldehyde, glutaraldehyde, acetaldehyde and epoxy isopropyl alcohol form.
According to an embodiment of the present invention, this polymerization reaction time is 60 minutes to 180 minutes.
Compared with prior art, the present invention uses the combination of different chitosan fibers, and amine groups content is higher, can attract electronegative platelet, accelerates wound hemostasis speed, and through acidify and crosslinking Treatment, still maintains heavily stressed intensity after using.Dressing of the present invention has heavily stressed intensity, except can be used for general hemostasis, also can be used for the wrapping of wound, having haemostatic effect and stress intensity concurrently, can really realize object of the present invention.
Accompanying drawing explanation
Fig. 1 is the manufacturing flow chart of the preferred embodiment of bleeding-stopping dressing of the present invention.
Detailed description of the invention
For making there is further understanding to object of the present invention, structure, feature and function thereof, embodiment is hereby coordinated to be described in detail as follows.
In order to make describing of this disclosure more detailed and complete, hereafter have been directed to embodiments of the present invention and specific embodiment proposes illustrative description; But this is not the unique forms implementing or use the specific embodiment of the invention.Each embodiment disclosed below, can combine mutually or replace, also can add other embodiment in one embodiment, and need not further record or illustrate useful when.
In one embodiment of the present invention, provide a kind of bleeding-stopping dressing, and this bleeding-stopping dressing is because of containing more amido, therefore there is preferably hemostatic capability, and still can maintain heavily stressed intensity in use.
Bleeding-stopping dressing provided by the present invention can comprise percentage by weight be 25% to 50% the chitosan fiber through acidification and percentage by weight be 50% to 75% the chitosan fiber through crosslinking Treatment.
In one embodiment of this invention, aforementioned bleeding-stopping dressing, the dry tensile strength when drying is 1000 gram force to 2600 gram forces, and wet tensile strength time after using in moisture state is then 200 gram force to 600 gram forces.Liquid Absorption rate is 14% to 18%.The weight content of amido is 50% to 90%.
Again, in another embodiment of the present invention, the invention provides a kind of manufacture method of bleeding-stopping dressing, and the bleeding-stopping dressing that method is formed thus has good hemostatic capability, and still can maintain heavily stressed intensity after a procedure.
Preceding method can comprise the steps, but not as limit.
Please refer to Fig. 1, Fig. 1 is the manufacturing flow chart of the preferred embodiment of bleeding-stopping dressing of the present invention.First, in the step s 100, chitosan fiber is dissolved in acidic aqueous, forms acidify chitosan fiber solution.In this step, optionally with blender agitating solution, in one embodiment of this invention, mixing time can be about 2 hours.Then, in step s 200, aforementioned acidify chitin fiber solution is carried out dewatering and drying and processing, form acidify chitosan fiber.Aforementioned temperature of carrying out drying and processing is 70 DEG C to 80 DEG C, and the time of drying and processing is 160 minutes to 200 minutes.In a preferred embodiment of the present invention, the temperature of carrying out drying and processing is about 75 DEG C, and the time of drying and processing is about 180 minutes.
The Deacetylation degree of aforementioned chitosan fiber is 80% to 99%.Acidic aqueous comprises organic solvent and acid solution, and applicable acid solution comprises phosphoric acid, citric acid, malic acid, succinic acid, acetic acid, hydrochloric acid and lactic acid, and pH value is 2 to 6, is preferably 3 to 5, is more preferred from 3.5 to 5.5.In a specific embodiment of the present invention, aforementioned acid organic solution comprises acetic acid and ethanol, and pH value approximates 4.7.
Meanwhile, as shown in step S300, chitosan fiber and cross-linking agent are dissolved in organic solvent, after polyreaction, form crosslinked chitosan fiber solution.Wherein, polymerization reaction time is 60 minutes to 180 minutes.In this step, optionally with blender agitating solution.In a preferred embodiment of the present invention, the response time can be about 120 minutes.Then, in step S400, crosslinked chitosan fiber solution is carried out dewatering and drying and processing, form crosslinked chitosan fiber.In an embodiment of the present invention, the temperature of carrying out drying and processing is 75 DEG C, and the time of drying and processing is 180 minutes.
The Deacetylation degree of aforementioned chitosan is 80% to 99%.Aforementioned applicable organic solvent is selected from the group that methanol, ethanol, acetone and isopropyl alcohol form.The cross-linking agent be applicable to is selected from the group that formaldehyde, glutaraldehyde, acetaldehyde and epoxy isopropyl alcohol form.In a specific embodiment of the present invention, organic solvent can be ethanol, and cross-linking agent can be glutaraldehyde.
Finally, in step S500, with tailor's cushion method, aforementioned acidify chitosan fiber and aforementioned crosslinked chitosan fiber are made chitosan adhesive-bonded fabric.
Aforementioned tailor's cushion method becomes the chitosan fiber of puffy fibre web after thrusting oven dry with pricker, utilize hook thorn some the chitosan fibers with firmly fibre web on pricker, it is made to produce displacement with pricker through fibre web, due to rubbing action, fibre web is compressed simultaneously, after thrusting certain depth, pricker gos up, by this hook thorn forward, these chitosan fibers are departed from hook thorn, and stay fine meshy surface with plumbness, make tightly to roll mutually between chitosan fiber and fiber to be combined, to make chitosan adhesive-bonded fabric.In one embodiment of this invention, aforementioned chitosan adhesive-bonded fabric comprise percentage by weight be 25% acidify chitosan fiber and percentage by weight be 75% crosslinked chitosan fiber.In another embodiment of the invention, aforementioned chitosan adhesive-bonded fabric comprise percentage by weight be 50% acidify chitosan fiber and percentage by weight be 50% crosslinked chitosan fiber.
With the bleeding-stopping dressing manufactured by manufacture method of the present invention, there is preferably hemostatic capability and can maintain the characteristics such as heavily stressed intensity.
Under be classified as the preparation of bleeding-stopping dressing of the present invention:
Acidic aqueous (pH is about 4.7) is formed after the ethanol mixing of the acetic acid of 1000 grams and 19000 grams, again the chitosan fiber of 300 grams (is purchased from this mole of sea, China) be dissolved in acidic aqueous, stir 2 hours, form acidify chitosan fiber solution.Then, fiber is put into dewaterer, centrifugation time is 2 minutes, carries out processed.Again fiber is put into baking oven, design temperature is 75 DEG C and dries after 180 minutes, forms acidify chitosan fiber.
By the cross-linking agent glutaraldehyde of 300 grams of chitosan fibers (be purchased from sea this mole, China) and 50 grams, be dissolved in the alcoholic solution that 10000 grams of concentration are 0.5% concentration.After stirring, reacting 2 hours, form crosslinked chitosan fiber solution.Then, fiber is put into dewaterer, centrifugation time is 2 minutes, carries out processed.Again fiber is put into baking oven, design temperature is 75 DEG C and dries after 180 minutes, forms crosslinked chitosan fiber.
Then, with tailor's cushion method, ratio shown in list 1 below acidify chitosan fiber and crosslinked chitosan fiber is made adhesive-bonded fabric, table 1 is the Detailed composition of embodiment 1 and embodiment 2.
Table 1: the Detailed composition of embodiment 1 and embodiment 2
Under be classified as bleeding-stopping dressing of the present invention physical property test:
(1) dry tensile strength method of testing:
Loading onto 50 kilograms of load measuring gauges with puller system and setting test speed is 200 mm/min (mm/min), after bleeding-stopping dressing fracture, and the maximum pull of record breakaway poing.
(2) wet tensile strength method of testing:
Bleeding-stopping dressing is made to draw 3 milliliters of saline solutions, then with the maximum pull of above-mentioned tensile test condition record breakaway poing.
(3) Liquid Absorption rate method of testing:
Measure dry hemostatic dressing weight (W1), dry hemostatic dressing and the heavy saline solution of its weight 40 times to be together placed in the baking oven of 37 DEG C 30 minutes.Then measure wet bleeding-stopping dressing weight (W2) and substitute into following formula and obtain Liquid Absorption rate.
Liquid Absorption rate=(W2-W1)/W1 × 100%
(4) amine groups content method of testing:
The ethanol being 95% by the toluidine blue of 0.05 gram (Toluidine blue) and 49.95 grams of concentration fully dissolves, as indicator in beaker.Then the acetum being 5% by the bleeding-stopping dressing of 0.5 gram and 99.5 grams of concentration is inserted in beaker and is fully dissolved, and forms chitosan solution.Get 1 gram of aforementioned chitosan solution and 30 grams of pure water again to insert beaker and be fully mixed into solution to be measured.Six aforementioned indicators added in solution to be measured, solution presents blueness, then is titrated to solution with polyvinyl sulfate potassium (POLYVINYLSULFURIC ACID POTASSIUM SALT, PVSK) and presents purple.Then titration volumes is substituted into following formula and namely obtain amine groups content.Wherein, DD% is Deacetylation degree (DD%).
f=PVSK solution concentration correction coefficient
(5) clotting ability method of testing:
By New Zealand's White Rabbit ear venous puncture 50 milliliters of blood, be isolated platelet suspension through centrifugal purification.Then get 0.6 milliliter of platelet suspension and put into Special test tube, and add Magnetitum with accelerated reaction, again 1 milligram of bleeding-stopping dressing is placed in above Special test tube and also immerses platelet suspension completely, react after 20 minutes, then test tube is put into Platelet Aggregation Assay instrument utilize turbidimetry analyze platelet aggregation rate.
The various embodiments described above are carried out above-mentioned physical property test.Test result is shown in lower list 2, and table 2 is the physical property test result of embodiment.
Table 2: the physical property test result of embodiment
Result as shown in Table 2 can be learnt, bleeding-stopping dressing of the present invention has preferably Liquid Absorption rate, amine groups content and platelet aggregation rate, shows bleeding-stopping dressing of the present invention and has splendid hemostatic capability.In addition, from embodiment, bleeding-stopping dressing of the present invention has the dry tensile strength of at least 1129 gram forces in drying regime, and also can maintain the wet tensile strength of at least 249 gram forces at moisture state.
From the above, by bleeding-stopping dressing of the present invention, there is high clotting ability and splendid physical characteristic, especially can also maintain heavily stressed intensity when moisture state.
Although the present invention discloses as above with preferred embodiment; so itself and be not used to limit the present invention, any those skilled in the art, without departing from the spirit and scope of the present invention; when doing a little change and retouching, therefore protection scope of the present invention is when being as the criterion depending on claim.
Claims (10)
1. a bleeding-stopping dressing, is characterized in that this bleeding-stopping dressing comprises:
Percentage by weight is the chitosan fiber through acidification of 25% to 50%; And
Percentage by weight is the chitosan fiber through crosslinking Treatment of 50% to 75%.
2. bleeding-stopping dressing as claimed in claim 1, it is characterized in that the dry tensile strength of this bleeding-stopping dressing is 1000 gram force to 2600 gram forces, and the wet tensile strength of this bleeding-stopping dressing is 200 gram force to 600 gram forces.
3. bleeding-stopping dressing as claimed in claim 1, is characterized in that the weight content of the amido of this bleeding-stopping dressing is 50% to 90%.
4. a manufacture method for bleeding-stopping dressing, is characterized in that the method comprises:
A chitosan fiber is dissolved in acidic aqueous by (), form acidify chitosan fiber solution;
B this acidify chitosan fiber solution carries out dewatering and drying and processing by (), form acidify chitosan fiber;
C chitosan fiber and cross-linking agent are dissolved in organic solvent by (), form crosslinked chitosan fiber solution after polyreaction;
D this crosslinked chitosan fiber solution carries out dewatering and drying and processing by (), form crosslinked chitosan fiber; And
E this acidify chitosan fiber and this crosslinked chitosan fiber are made chitosan adhesive-bonded fabric with tailor's cushion method by ();
Wherein, this chitosan adhesive-bonded fabric comprise percentage by weight be 25% to 50% this acidify chitosan fiber and percentage by weight be 50% to 75% this crosslinked chitosan fiber.
5. method as claimed in claim 4, is characterized in that the Deacetylation degree of this chitosan is 80% to 99%.
6. method as claimed in claim 4, is characterized in that this acidic aqueous comprises organic solvent and acid solution; Wherein, this acid solution is selected from the group that phosphoric acid, citric acid, malic acid, succinic acid, acetic acid, hydrochloric acid and lactic acid form.
7. method as claimed in claim 4, is characterized in that the pH value of this acidic aqueous is 2 to 6.
8. method as claimed in claim 4, is characterized in that the group that this organic solvent is selected from methanol, ethanol, acetone and isopropyl alcohol and forms.
9. method as claimed in claim 4, is characterized in that the group that this cross-linking agent is selected from formaldehyde, glutaraldehyde, acetaldehyde and epoxy isopropyl alcohol and forms.
10. method as claimed in claim 4, is characterized in that this polymerization reaction time is 60 minutes to 180 minutes.
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| CN201310315115.5A CN103480029B (en) | 2013-07-25 | 2013-07-25 | Haemostatic dressing and production method thereof |
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| CN201310315115.5A CN103480029B (en) | 2013-07-25 | 2013-07-25 | Haemostatic dressing and production method thereof |
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| CN103480029B true CN103480029B (en) | 2015-03-11 |
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| IL273876B2 (en) * | 2020-04-07 | 2024-02-01 | Reddress Ltd | Method and system for forming a blood clot-based wound dressing, and a coupling device enabling it |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1493364A (en) * | 2002-10-30 | 2004-05-05 | 郑婵颖 | Manufacturing method of chitin biodressing |
| CN1938036A (en) * | 2004-02-23 | 2007-03-28 | 洛马林达大学医学中心 | Hemostatic agent for topical and internal use |
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| GB2463523B (en) * | 2008-09-17 | 2013-05-01 | Medtrade Products Ltd | Wound care device |
| US8298584B2 (en) * | 2008-12-30 | 2012-10-30 | Collagen Matrix, Inc. | Biopolymeric membrane for wound protection and repair |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1493364A (en) * | 2002-10-30 | 2004-05-05 | 郑婵颖 | Manufacturing method of chitin biodressing |
| CN1938036A (en) * | 2004-02-23 | 2007-03-28 | 洛马林达大学医学中心 | Hemostatic agent for topical and internal use |
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