CN103480029A - Haemostatic dressing and production method thereof - Google Patents
Haemostatic dressing and production method thereof Download PDFInfo
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- CN103480029A CN103480029A CN201310315115.5A CN201310315115A CN103480029A CN 103480029 A CN103480029 A CN 103480029A CN 201310315115 A CN201310315115 A CN 201310315115A CN 103480029 A CN103480029 A CN 103480029A
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Abstract
The invention provides a haemostatic dressing and a production method thereof. The haemostatic dressing comprises 25 to 50 wt% of a chitosan fiber having undergone acid treatment and 50 to 75 wt% of a chitosan fiber having undergone cross-linking treatment. The haemostatic dressing provided by the invention has an excellent haemostatic effect and can maintain high stress intensity in use.
Description
Technical field
The present invention is about a kind of bleeding-stopping dressing and manufacture method thereof, and particularly about a kind of bleeding-stopping dressing that comprises the chitosan fiber and manufacture method thereof.
Background technology
Chitosan (Chitosan) is the macromolecule by chitin (Chitin) gained after deacetylation; chitin is the natural polymer extracted from biology, mainly is present in Crustacean (shrimp, Eriocheir sinensis), insecticide shell or the cell wall of Mycophyta.Chitin is the straight chain shape macromolecule polysaccharide polymer formed with β-Isosorbide-5-Nitrae bond by glucamine and N-Acetyl-D-glucosamine.Chitosan is because having good biocompatibility, biological activity, no cytotoxicity and Biodegradable with iuntercellular, therefore can be applicable to biomaterial and medical material.Chitosan with positive electricity, therefore can attract platelet aggregation, and then reach the effect of quick-acting haemostatic powder in acid solution.
At present, the dressing formed with the chitosan adhesive-bonded fabric is extensively in clinical middle application, yet chitosan dressing has the not good shortcoming of stress, and after contact blood, meeting forms gel fast and ruptures.In correlative technology field, existed some to increase the method for bleeding-stopping dressing stress intensity.For example, U.S. Pat 7,981,872 exposure utilize rayon (rayon) fiber to meet the toughness that water-insoluble characteristic maintains chitosan dressing, and then improve the not good problem of chitosan dressing stress intensity.The open case US2011/0236433 of United States Patent (USP) discloses the adhesive-bonded fabric dressing that becomes to contain two kinds of fibrous materials in conjunction with chitosan fiber and cellulose (cellulose) fiber combinations, be also to utilize the water-fast high-strength characteristic of cellulose fibre itself, maintain the tensile strength of dressing when patient's wound is used.Yet, can maintain dressing stress though add another to meet water-insoluble material, also can reduce the ratio of chitosan fiber, and then cause the dressing hemostatic capability to reduce simultaneously.Therefore, how to improve the chitosan proportion of fibers, maintain the stress intensity after water is met in dressing simultaneously, be still the problem that need overcome.
Summary of the invention
Therefore, one of purpose of the present invention is to provide a kind of bleeding-stopping dressing and manufacture method thereof of novelty, so that bleeding-stopping dressing has haemostatic effect and stress intensity concurrently, to improve shortcoming of the prior art.
The invention provides a kind of bleeding-stopping dressing, comprising: the fiber of the chitosan through crosslinking Treatment that the fiber of the chitosan through acidification that percentage by weight is 25% to 50% and percentage by weight are 50% to 75%.
According to an embodiment of the present invention, the dry tensile strength of this bleeding-stopping dressing is 1000 gram force to 2600 gram forces, and wet tensile strength is 200 gram force to 600 gram forces.
According to an embodiment of the present invention, the amido weight content of this bleeding-stopping dressing is 50% to 90%.
Another object of the present invention provides a kind of manufacture method of bleeding-stopping dressing, comprising:
(a) the chitosan fiber is dissolved in acid organic solution, forms acidify chitosan fiber solution;
(b) acidify chitosan solution is dewatered and drying and processing, formed acidify chitosan fiber;
(c) chitosan fiber and cross-linking agent are dissolved in organic solvent, form crosslinked chitosan fiber solution after polyreaction;
(d) crosslinked chitosan fiber solution is dewatered and drying and processing, formed crosslinked chitosan fiber; And
(e) with the tailor's cushion method, acidify chitosan fiber and crosslinked chitosan fiber are made to the chitosan adhesive-bonded fabric;
Wherein, the chitosan adhesive-bonded fabric comprises the crosslinked chitosan fiber that acidify chitosan fiber that percentage by weight is 25% to 50% and percentage by weight are 50% to 75%.
According to an embodiment of the present invention, the Deacetylation degree of this chitosan fiber is 80% to 99%.
According to an embodiment of the present invention, this acidity organic solution comprises organic solvent and acid solution; Wherein, this acid solution is selected from the group that phosphoric acid, citric acid, malic acid, succinic acid, acetic acid, hydrochloric acid and lactic acid form.
According to an embodiment of the present invention, the pH value of this acidity organic solution is 2 to 6.
According to an embodiment of the present invention, this organic solvent is selected from the group that methanol, ethanol, acetone and isopropyl alcohol form.
According to an embodiment of the present invention, this cross-linking agent is selected from the group that formaldehyde, glutaraldehyde, acetaldehyde and epoxy isopropyl alcohol form.
According to an embodiment of the present invention, this polymerization reaction time is 60 minutes to 180 minutes.
Compared with prior art, the present invention uses the combination of different chitosan fibers, and amido content is higher, can attract electronegative platelet, accelerates wound hemostasis speed, and, through acidify and crosslinking Treatment, still maintains heavily stressed intensity after use.Dressing of the present invention has heavily stressed intensity, except can be used for general hemostasis, also can be used for the wrapping of wound, has haemostatic effect and stress intensity concurrently, can really realize purpose of the present invention.
The accompanying drawing explanation
The manufacturing flow chart of the preferred embodiment that Fig. 1 is bleeding-stopping dressing of the present invention.
The specific embodiment
For making that purpose of the present invention, structure, feature and function thereof are had to further understanding, hereby coordinate embodiment to be described in detail as follows.
For the narration that makes this disclosure more detailed and complete, hereinafter for embodiments of the present invention and specific embodiment illustrative description has been proposed; But this is not unique form of implementing or using the specific embodiment of the invention.Below disclosed each embodiment, can combine mutually or replace under useful situation, also can add in one embodiment other embodiment, and need not further put down in writing or illustrate.
In one embodiment of the present invention, provide a kind of bleeding-stopping dressing, and this bleeding-stopping dressing is because containing more amido, thus preferably hemostatic capability there is, and still can maintain heavily stressed intensity in use.
Bleeding-stopping dressing provided by the present invention can comprise the fiber of the chitosan through crosslinking Treatment that the fiber of the chitosan through acidification that percentage by weight is 25% to 50% and percentage by weight are 50% to 75%.
In one embodiment of this invention, aforementioned bleeding-stopping dressing, the dry tensile strength when drying is 1000 gram force to 2600 gram forces, the wet tensile strength while after use, being moisture state is 200 gram force to 600 gram forces.The Liquid Absorption rate is 14% to 18%.The weight content of amido is 50% to 90%.
In another embodiment of the present invention, the invention provides a kind of manufacture method of bleeding-stopping dressing, and the formed bleeding-stopping dressing of method has good hemostatic capability thus, and still can maintain heavily stressed intensity after use again.
Preceding method can comprise the steps, but not as limit.
Please refer to Fig. 1, the manufacturing flow chart of the preferred embodiment that Fig. 1 is bleeding-stopping dressing of the present invention.At first, in step S100, the chitosan fiber is dissolved in acid organic solution, forms acidify chitosan fiber solution.In this step, optionally with the blender agitating solution, in one embodiment of this invention, mixing time can be approximately 2 hours.Then, in step S200, aforementioned acidify chitin fiber solution is dewatered and drying and processing, formed acidify chitosan fiber.Aforementioned temperature of carrying out drying and processing is 70 ℃ to 80 ℃, and the time of drying and processing is 160 minutes to 200 minutes.In a preferred embodiment of the present invention, the temperature of carrying out drying and processing is approximately 75 ℃, and the time of drying and processing is approximately 180 minutes.
The Deacetylation degree of aforementioned chitosan fiber is 80% to 99%.Acid organic solution comprises organic solvent and acid solution, and applicable acid solution comprises phosphoric acid, citric acid, malic acid, succinic acid, acetic acid, hydrochloric acid and lactic acid, and pH value is 2 to 6, is preferably 3 to 5, is more preferred from 3.5 to 5.5.In a specific embodiment of the present invention, aforementioned acid organic solution comprises acetic acid and ethanol, and pH value approximates 4.7.
, as shown in step S300, chitosan fiber and cross-linking agent are dissolved in organic solvent simultaneously, form crosslinked chitosan fiber solution after polyreaction.Wherein, polymerization reaction time is 60 minutes to 180 minutes.In this step, optionally with the blender agitating solution.In a preferred embodiment of the present invention, the response time can be approximately 120 minutes.Then, in step S400, crosslinked chitosan fiber solution is dewatered and drying and processing, formed crosslinked chitosan fiber.In an embodiment of the present invention, the temperature of carrying out drying and processing is 75 ℃, and the time of drying and processing is 180 minutes.
The Deacetylation degree of aforementioned chitosan is 80% to 99%.Aforementioned applicable organic solvent is selected from the group that methanol, ethanol, acetone and isopropyl alcohol form.Applicable cross-linking agent is selected from the group that formaldehyde, glutaraldehyde, acetaldehyde and epoxy isopropyl alcohol form.In a specific embodiment of the present invention, organic solvent can be ethanol, and cross-linking agent can be glutaraldehyde.
Finally, in step S500, with the tailor's cushion method, aforementioned acidify chitosan fiber and aforementioned crosslinked chitosan fiber are made to the chitosan adhesive-bonded fabric.
Aforementioned tailor's cushion method is thrust after oven dry the chitosan fiber that becomes the puffy fibre web with pricker, utilize the hook thorn on pricker to be with firmly some chitosan fibers of fibre web, make it with pricker, through fibre web, produce displacement, because making fibre web, rubbing action is compressed simultaneously, after thrusting certain depth, the thorn needle pickup, by this hook thorn forward, these chitosan fibers are broken away to the hook thorn, and stay fibre web shape surface with plumbness, make tightly to roll and be combined mutually between chitosan fiber and fiber, to make the chitosan adhesive-bonded fabric.In one embodiment of this invention, aforementioned chitosan adhesive-bonded fabric comprises the crosslinked chitosan fiber that acidify chitosan fiber that percentage by weight is 25% and percentage by weight are 75%.In another embodiment of the present invention, aforementioned chitosan adhesive-bonded fabric comprises the crosslinked chitosan fiber that acidify chitosan fiber that percentage by weight is 50% and percentage by weight are 50%.
The bleeding-stopping dressing manufactured with manufacture method of the present invention has preferably hemostatic capability and can maintain the characteristics such as heavily stressed intensity.
Under classify the preparation of bleeding-stopping dressing of the present invention as:
After mixing, the ethanol of the acetic acid of 1000 grams and 19000 grams forms acid organic solution (pH is about 4.7), again the chitosan fiber of 300 grams (is purchased to this mole of sea, China) be dissolved in acid organic solution, stir 2 hours, form acidify chitosan fiber solution.Then, fiber is put into to dewaterer, centrifugation time is 2 minutes, carries out processed.Again fiber is put into to baking oven, design temperature is 75 ℃ and dries after 180 minutes, forms acidify chitosan fiber.
By the cross-linking agent glutaraldehyde of 300 gram chitosan fibers (be purchased from sea this mole, China) and 50 grams, be dissolved in the alcoholic solution that 10000 gram concentration are 0.5% concentration.After stirring, reacting 2 hours, form crosslinked chitosan fiber solution.Then, fiber is put into to dewaterer, centrifugation time is 2 minutes, carries out processed.Again fiber is put into to baking oven, design temperature is 75 ℃ and dries after 180 minutes, forms crosslinked chitosan fiber.
Then, with the tailor's cushion method, ratio shown in acidify chitosan fiber and the following list 1 of crosslinked chitosan fiber is made to adhesive-bonded fabric, the detailed formula that table 1 is embodiment 1 and embodiment 2.
The detailed formula of table 1: embodiment 1 and embodiment 2
Under classify the physical property test of bleeding-stopping dressing of the present invention as:
(1) dry tensile strength method of testing:
The puller system of take is loaded onto 50 kilograms of load measuring gauges and is set test speed as 200 mm/min (mm/min), after the bleeding-stopping dressing fracture, records the maximum pull of breakaway poing.
(2) wet tensile strength method of testing:
Make bleeding-stopping dressing draw 3 milliliters of saline solutions, then record the maximum pull of breakaway poing with above-mentioned tensile test condition.
(3) Liquid Absorption rate method of testing:
Measure dry hemostatic dressing weight (W1), the saline solution of 40 times heavy of dry hemostatic dressing and its weight together is placed in the baking oven of 37 ℃ to 30 minutes.Then measure wet bleeding-stopping dressing weight (W2) the following formula of substitution and obtain the Liquid Absorption rate.
Liquid Absorption rate=(W2-W1)/W1 * 100%
(4) amido content test method:
The ethanol that is 95% by the toluidine blue of 0.05 gram (Toluidine blue) and 49.95 gram concentration fully dissolves in beaker, as indicator.The acetum that is then 5% by the bleeding-stopping dressing of 0.5 gram and 99.5 gram concentration is inserted in beaker and is fully dissolved, and forms chitosan solution.Getting the aforementioned chitosan solution of 1 gram and 30 gram pure water inserts beaker and fully is mixed into solution to be measured again.Six aforementioned indicators are added in solution to be measured, and solution presents blueness, then is titrated to solution with sulphuric acid polyethylene potassium (POLYVINYLSULFURIC ACID POTASSIUM SALT, PVSK) and presents purple.Then the following formula of titration volume substitution is obtained to amido content.Wherein, DD% is Deacetylation degree (DD%).
(5) blood coagulation aptitude tests method:
Extract 50 milliliters of blood by New Zealand's White Rabbit ear vein, through centrifugal purification, be isolated platelet suspension.Then get 0.6 milliliter of platelet suspension and put into Special test tube, and add Magnetitum with accelerated reaction, again 1 milligram of bleeding-stopping dressing be placed in to the Special test tube top and immerse platelet suspension fully, react after 20 minutes, then test tube is put into to the platelet aggregation analyser and utilize turbidimetry to analyze platelet aggregation rate.
The various embodiments described above are carried out to above-mentioned physical property test.Test result is shown in lower list 2, the physical property test result that table 2 is embodiment.
The physical property test result of table 2: embodiment
Result as shown in Table 2 can learn, bleeding-stopping dressing of the present invention has preferably Liquid Absorption rate, amido content and platelet aggregation rate, shows that bleeding-stopping dressing of the present invention has splendid hemostatic capability.In addition, from embodiment, bleeding-stopping dressing of the present invention has the dry tensile strength of at least 1129 gram forces in drying regime, and also can maintain the wet tensile strength of at least 249 gram forces at moisture state.
From the above, by bleeding-stopping dressing of the present invention, there is high blood coagulation ability and splendid physical characteristic, especially when moisture state, can also maintain heavily stressed intensity.
Although the present invention discloses as above with preferred embodiment; so it is not in order to limit the present invention, any those skilled in the art, without departing from the spirit and scope of the present invention; when doing a little change and retouching, so protection scope of the present invention is as the criterion when looking claim.
Claims (10)
1. a bleeding-stopping dressing is characterized in that this bleeding-stopping dressing comprises:
The fiber of the chitosan through acidification that percentage by weight is 25% to 50%; And
The fiber of the chitosan through crosslinking Treatment that percentage by weight is 50% to 75%.
2. bleeding-stopping dressing as claimed in claim 1, the dry tensile strength that it is characterized in that this bleeding-stopping dressing is 1000 gram force to 2600 gram forces, and the wet tensile strength of this bleeding-stopping dressing is 200 gram force to 600 gram forces.
3. bleeding-stopping dressing as claimed in claim 1, the weight content that it is characterized in that the amido of this bleeding-stopping dressing is 50% to 90%.
4. the manufacture method of a bleeding-stopping dressing is characterized in that the method comprises:
(a) the chitosan fiber is dissolved in acid organic solution, forms acidify chitosan fiber solution;
(b) this acidify chitosan fiber solution is dewatered and drying and processing, formed acidify chitosan fiber;
(c) chitosan fiber and cross-linking agent are dissolved in organic solvent, form crosslinked chitosan fiber solution after polyreaction;
(d) this crosslinked chitosan fiber solution is dewatered and drying and processing, formed crosslinked chitosan fiber; And
(e) with the tailor's cushion method, this acidify chitosan fiber and this crosslinked chitosan fiber are made to the chitosan adhesive-bonded fabric;
Wherein, this chitosan adhesive-bonded fabric comprises this crosslinked chitosan fiber that this acidify chitosan fiber that percentage by weight is 25% to 50% and percentage by weight are 50% to 75%.
5. method as claimed in claim 4, the Deacetylation degree that it is characterized in that this chitosan is 80% to 99%.
6. method as claimed in claim 4, is characterized in that this acidity organic solution comprises organic solvent and acid solution; Wherein, this acid solution is selected from the group that phosphoric acid, citric acid, malic acid, succinic acid, acetic acid, hydrochloric acid and lactic acid form.
7. method as claimed in claim 4, the pH value that it is characterized in that this acidity organic solution is 2 to 6.
8. method as claimed in claim 4, is characterized in that this organic solvent is selected from the group that methanol, ethanol, acetone and isopropyl alcohol form.
9. method as claimed in claim 4, is characterized in that this cross-linking agent is selected from the group that formaldehyde, glutaraldehyde, acetaldehyde and epoxy isopropyl alcohol form.
10. method as claimed in claim 4, is characterized in that this polymerization reaction time is 60 minutes to 180 minutes.
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| CN201310315115.5A CN103480029B (en) | 2013-07-25 | 2013-07-25 | Haemostatic dressing and production method thereof |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115397377A (en) * | 2020-04-07 | 2022-11-25 | 雷德助莱斯有限公司 | Blood extraction |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1493364A (en) * | 2002-10-30 | 2004-05-05 | 郑婵颖 | Manufacturing method of chitin biodressing |
| CN1938036A (en) * | 2004-02-23 | 2007-03-28 | 洛马林达大学医学中心 | Hemostatic agent for topical and internal use |
| US20100166823A1 (en) * | 2008-12-30 | 2010-07-01 | Collagen Matrix, Inc. | Biopolymeric membrane for wound protection and repair |
| US20110236433A1 (en) * | 2008-09-17 | 2011-09-29 | Medtrade Products Limited | Wound care device |
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2013
- 2013-07-25 CN CN201310315115.5A patent/CN103480029B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1493364A (en) * | 2002-10-30 | 2004-05-05 | 郑婵颖 | Manufacturing method of chitin biodressing |
| CN1938036A (en) * | 2004-02-23 | 2007-03-28 | 洛马林达大学医学中心 | Hemostatic agent for topical and internal use |
| US20110236433A1 (en) * | 2008-09-17 | 2011-09-29 | Medtrade Products Limited | Wound care device |
| US20100166823A1 (en) * | 2008-12-30 | 2010-07-01 | Collagen Matrix, Inc. | Biopolymeric membrane for wound protection and repair |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115397377A (en) * | 2020-04-07 | 2022-11-25 | 雷德助莱斯有限公司 | Blood extraction |
| CN115397377B (en) * | 2020-04-07 | 2023-08-29 | 雷德助莱斯有限公司 | blood extraction |
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