CN103477981A - Acer palmatum Seiryu tissue culture propagation process - Google Patents
Acer palmatum Seiryu tissue culture propagation process Download PDFInfo
- Publication number
- CN103477981A CN103477981A CN201310402032.XA CN201310402032A CN103477981A CN 103477981 A CN103477981 A CN 103477981A CN 201310402032 A CN201310402032 A CN 201310402032A CN 103477981 A CN103477981 A CN 103477981A
- Authority
- CN
- China
- Prior art keywords
- bud
- cultivate
- seiryu
- acer palmatum
- tissue culture
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 241000931515 Acer palmatum Species 0.000 title claims abstract description 31
- 238000000034 method Methods 0.000 title claims abstract description 16
- 230000001954 sterilising effect Effects 0.000 claims abstract description 16
- 239000012879 subculture medium Substances 0.000 claims abstract description 11
- 238000005520 cutting process Methods 0.000 claims abstract description 9
- 201000010099 disease Diseases 0.000 claims abstract description 6
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 claims abstract description 6
- 238000012258 culturing Methods 0.000 claims description 15
- 238000005286 illumination Methods 0.000 claims description 15
- 239000000463 material Substances 0.000 claims description 15
- 239000002609 medium Substances 0.000 claims description 10
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 5
- 229960002523 mercuric chloride Drugs 0.000 claims description 5
- LWJROJCJINYWOX-UHFFFAOYSA-L mercury dichloride Chemical compound Cl[Hg]Cl LWJROJCJINYWOX-UHFFFAOYSA-L 0.000 claims description 5
- 230000035755 proliferation Effects 0.000 claims description 5
- 239000008223 sterile water Substances 0.000 claims description 5
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 5
- 230000001939 inductive effect Effects 0.000 abstract description 4
- 239000001963 growth medium Substances 0.000 abstract description 3
- 238000009825 accumulation Methods 0.000 abstract description 2
- 238000004519 manufacturing process Methods 0.000 abstract description 2
- 241000700605 Viruses Species 0.000 abstract 1
- 230000035784 germination Effects 0.000 abstract 1
- 239000012883 rooting culture medium Substances 0.000 abstract 1
- 238000010008 shearing Methods 0.000 abstract 1
- 241000208140 Acer Species 0.000 description 13
- 241001143500 Aceraceae Species 0.000 description 5
- 238000010586 diagram Methods 0.000 description 4
- 241000196324 Embryophyta Species 0.000 description 3
- 238000009395 breeding Methods 0.000 description 3
- 230000001488 breeding effect Effects 0.000 description 3
- 241000219227 Acer japonicum Species 0.000 description 2
- UUTKICFRNVKFRG-WDSKDSINSA-N (4R)-3-[oxo-[(2S)-5-oxo-2-pyrrolidinyl]methyl]-4-thiazolidinecarboxylic acid Chemical compound OC(=O)[C@@H]1CSCN1C(=O)[C@H]1NC(=O)CC1 UUTKICFRNVKFRG-WDSKDSINSA-N 0.000 description 1
- 235000015988 Acer ginnala Nutrition 0.000 description 1
- 244000046151 Acer negundo Species 0.000 description 1
- 235000004422 Acer negundo Nutrition 0.000 description 1
- 240000004731 Acer pseudoplatanus Species 0.000 description 1
- 235000002754 Acer pseudoplatanus Nutrition 0.000 description 1
- 241000317410 Arisaema dracontium Species 0.000 description 1
- 235000002239 Dracunculus vulgaris Nutrition 0.000 description 1
- NNBFNNNWANBMTI-UHFFFAOYSA-M brilliant green Chemical group OS([O-])(=O)=O.C1=CC(N(CC)CC)=CC=C1C(C=1C=CC=CC=1)=C1C=CC(=[N+](CC)CC)C=C1 NNBFNNNWANBMTI-UHFFFAOYSA-M 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 238000010276 construction Methods 0.000 description 1
- 230000035613 defoliation Effects 0.000 description 1
- 238000009826 distribution Methods 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 230000006698 induction Effects 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000035772 mutation Effects 0.000 description 1
- 230000000869 mutational effect Effects 0.000 description 1
- 238000004161 plant tissue culture Methods 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 230000008929 regeneration Effects 0.000 description 1
- 238000011069 regeneration method Methods 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 230000003612 virological effect Effects 0.000 description 1
Images
Landscapes
- Cultivation Of Plants (AREA)
Abstract
The invention discloses an acer palmatum Seiryu tissue culture propagation process which comprises the following steps: S1, collecting the current-year shoots of disease-free acer palmatum Seiryu, removing redundant leaves, shearing cutting into stem sections with buds, and sterilizing; S2, inoculating each sterilized stem section with the bud to a culture medium for growth, and inducing the germination of axillary buds; S3, carrying out subculture for multiplication, namely cutting off the germinating axillary buds, inoculating the cut germinated axillary buds on a subculture medium, and inducing the generation of multiple shoots; S4,carrying out rooting culture, cutting adventitious buds of the multiple shoots, inoculating on a rooting culture medium, and inducing the generation of a root. The acer palmatum Seiryu tissue culture propagation process disclosed by the invention can be used for constructing a Seiryu tissue culture propagation system, and realizing the breakthrough of the Seiryu propagation system, and is low in production cost, high in propagation coefficient, and short in propagation period, and is an effective way for the fast propagation of acer palmatum Seiryu and capable of preventing the virus accumulation, and by using the process, the tissue culture seedlings with consistent inheritable characters can be obtained.
Description
Technical field
The present invention relates to field of plant tissue culture technique, particularly Acer palmatum Green Dragon tissue culture propagation technique.
Background technology
Aceraceae (Aceraceae) maple belongs to (Acer) general term maple usually, and custom claims again " maple " or " maple ".Whole world maple has kind more than 200, is distributed in Asia, Europe, North America and Africa northern.China has more than 160 to plant, and accounts for more than 75% of whole world sum, and all there is distribution all parts of the country, the main product Yangtze river basin and on the south each provinces and regions.Maple can be used as construction timber, paper making raw material, medicine extraction, iundustrial oil and nectariferous plant.Tree-like abundant, the attitude of some maple graceful, leaf abundant, the leaf look changeable, is described as world-renowned ornamental foliage plant.Acer palmatum (Acer palmatum) has another name called Japanese maple, and genus Aceraceae, maple belong to defoliation small arbor or arbor.The tree performance grace is beautiful, leaf attractive in appearance, and the leaf look changeable, is famous ornamental tree species.Countries in the world are introducing and planting already, and mutation and modification are a lot.Japan's Acer palmatum is cultivated more than 450 reward leaf kinds at present.
China maple is viewed and admired the exploitation of resource and has compared very big-difference abroad.Maple is as important foliage tree kind, and supply falls short of demand for the cultivation nursery stock.Its breeding is main cuttage and the seed propagation method of adopting at present.Although the Aceraceae plant has very large capacity in the growth in large field, organize at present and train breed of less types.
In sum, less to the Study on tissue culture of Aceraceae plant both at home and abroad, only there is a few kind to set up comparatively perfect axil propagation Tissue Culture Regeneration System, as Acer pseudoplatanus, America Acer negundo, fine stern maple, Amur maple, Fructus Aceris davidii, compound leaf maple.And rarely have report for Acer palmatum and the kind thereof of cuttage root-taking difficulty.
The Acer palmatum Green Dragon (Acer palmatum ' Seiryu') is one of numerous Acer palmatum cultivation mutational varieties, its blade pinniform, and summer is emerald green, turns red, flourishing autumn, and strain shape grace, have much ornamental value.Be comparatively generally to reach precious fancy breed on market now, but also have no precedent report for the tissue culture propagation method of the Green Dragon.
Summary of the invention
The object of the invention is to overcome the shortcoming of prior art, the Acer palmatum Green Dragon tissue culture propagation that a kind of reproduction rate is high, reproduction speed is fast, easy and simple to handle technique is provided.
Purpose of the present invention is achieved through the following technical solutions: Acer palmatum Green Dragon tissue culture propagation technique, and it comprises the following steps:
S1, gather disease-free Acer palmatum Green Dragon current-year branch, remove unnecessary blade, be cut into stem with bud, stem with bud is first used alcohol sterilizing 30～60s, then uses mercuric chloride sterilizing 3～5min, then uses sterile water wash 4～6 times;
S2, stem with bud after sterilizing is inoculated on medium and grows, described medium is MS+NAA 0.3mg/L, and postvaccinal culture materials is placed in to intensity of illumination is that 2000～3000LX, light application time are to cultivate in 16h/d, the temperature culturing room that is 24～26 ℃, cultivate 2～3 weeks, induce axillary bud sprouting;
S3, shoot proliferation are cultivated, the axillalry bud of having sprouted is cut, be seeded on subculture medium, subculture medium is MS+6-BA 1.5mg/L+NAA0.3mg/L, and postvaccinal culture materials is placed in to intensity of illumination is that 2000～3000LX, light application time are to cultivate in 16h/d, the temperature culturing room that is 24～26 ℃, cultivate the induced bundle generation of sprouting 3～5 weeks;
S4, culture of rootage, indefinite bud on the cutting Multiple Buds, be inoculated on root media, root media is 1/2MS+NAA0.2mg/L, and postvaccinal culture materials is placed in to intensity of illumination is that 2000～3000LX, light application time are to cultivate in 16h/d, the temperature culturing room that is 24～26 ℃, cultivate 2～3 weeks, induce root to produce.
The present invention has the following advantages:
The present invention, for gathering Green Dragon stem explants, is just inducing axillalry bud to occur on the culture base, then the induced bundle generation of sprouting on proliferated culture medium, on the cutting Multiple Buds, indefinite bud enters root media, root induction, set up the tissue culture propagation system of the Green Dragon, realized the breakthrough of Green Dragon breeding system.
Low production cost of the present invention, the group obtained training seedling heredity shape is consistent, and reproduction coefficient is high, and the breeding cycle is short, is the fast numerous effective way of the Acer palmatum Green Dragon, and has avoided viral accumulation.
Culture medium prescription of the present invention is simple, and the cultivation flow process is simple and direct, easy and simple to handle, and culture efficiency is high, can be relatively easy to obtain Acer palmatum Green Dragon aseptic seedling, and hardening easily survives, time saving and energy saving.
The accompanying drawing explanation
Fig. 1 is the just schematic diagram of culture of the Acer palmatum Green Dragon of the present invention
Fig. 2 is the schematic diagram that Acer palmatum Green Dragon subculture of the present invention is cultivated
The schematic diagram that Fig. 3 is Acer palmatum Green Dragon culture of rootage of the present invention
The schematic diagram that Fig. 4 is Acer palmatum Green Dragon root system of the present invention.
Embodiment
Below in conjunction with drawings and Examples, the present invention will be further described, and protection scope of the present invention is not limited to the following stated:
embodiment 1:
Acer palmatum Green Dragon tissue culture propagation technique, it comprises the following steps:
S1, at fine day noon or afternoon, gather disease-free Acer palmatum Green Dragon current-year branch, remove unnecessary blade, be cut into stem with bud, stem with bud is first used alcohol sterilizing 30s, then uses mercuric chloride sterilizing 5min, then uses sterile water wash 4～6 times;
S2, stem with bud after sterilizing is inoculated on medium and grows, described medium is MS+NAA 0.3mg/L, and postvaccinal culture materials is placed in to intensity of illumination is that 2000～3000LX, light application time are to cultivate in 16h/d, the temperature culturing room that is 24～26 ℃, cultivate 15 days, induce axillary bud sprouting, as shown in Figure 1;
S3, shoot proliferation are cultivated, the axillalry bud of having sprouted is cut, be seeded on subculture medium, subculture medium is MS+6-BA 1.5mg/L+NAA0.3mg/L, and postvaccinal culture materials is placed in to intensity of illumination is that 2000～3000LX, light application time are to cultivate in 16h/d, the temperature culturing room that is 24～26 ℃, cultivate 30 days, the induced bundle generation of sprouting, as shown in Figure 2;
S4, culture of rootage, indefinite bud on the cutting Multiple Buds, be inoculated on root media, as shown in Figure 3, root media is 1/2MS+NAA0.2mg/L, and postvaccinal culture materials is placed in to intensity of illumination is that 2000～3000LX, light application time are to cultivate in 16h/d, the temperature culturing room that is 24～26 ℃, cultivates 15 day week, induce root to produce, as shown in Figure 4.
embodiment 2:
Acer palmatum Green Dragon tissue culture propagation technique, it comprises the following steps:
S1, at fine day noon or afternoon, gather disease-free Acer palmatum Green Dragon current-year branch, remove unnecessary blade, be cut into stem with bud, stem with bud is first used alcohol sterilizing 50s, then uses mercuric chloride sterilizing 4min, then uses sterile water wash 4～6 times;
S2, stem with bud after sterilizing is inoculated on medium and grows, described medium is MS+NAA 0.3mg/L, and postvaccinal culture materials is placed in to intensity of illumination is that 2000～3000LX, light application time are to cultivate in 16h/d, the temperature culturing room that is 24～26 ℃, cultivate 2 weeks, induce axillary bud sprouting, as shown in Figure 1;
S3, shoot proliferation are cultivated, the axillalry bud of having sprouted is cut, be seeded on subculture medium, subculture medium is MS+6-BA 1.5mg/L+NAA0.3mg/L, and postvaccinal culture materials is placed in to intensity of illumination is that 2000～3000LX, light application time are to cultivate in 16h/d, the temperature culturing room that is 24～26 ℃, cultivate 3 weeks, the induced bundle generation of sprouting, as shown in Figure 2;
S4, culture of rootage, indefinite bud on the cutting Multiple Buds, be inoculated on root media, as shown in Figure 3, root media is 1/2MS+NAA0.2mg/L, and postvaccinal culture materials is placed in to intensity of illumination is that 2000～3000LX, light application time are to cultivate in 16h/d, the temperature culturing room that is 24～26 ℃, cultivates 2 weeks, induce root to produce, as shown in Figure 4.
embodiment 3:
Acer palmatum Green Dragon tissue culture propagation technique, it comprises the following steps:
S1, at fine day noon or afternoon, gather disease-free Acer palmatum Green Dragon current-year branch, remove unnecessary blade, be cut into stem with bud, stem with bud is first used alcohol sterilizing 60s, then uses mercuric chloride sterilizing 3min, then uses sterile water wash 4～6 times;
S2, stem with bud after sterilizing is inoculated on medium and grows, described medium is MS+NAA 0.3mg/L, and postvaccinal culture materials is placed in to intensity of illumination is that 2000～3000LX, light application time are to cultivate in 16h/d, the temperature culturing room that is 24～26 ℃, cultivate 3 weeks, induce axillary bud sprouting, as shown in Figure 1;
S3, shoot proliferation are cultivated, the axillalry bud of having sprouted is cut, be seeded on subculture medium, subculture medium is MS+6-BA 1.5mg/L+NAA0.3mg/L, and postvaccinal culture materials is placed in to intensity of illumination is that 2000～3000LX, light application time are to cultivate in 16h/d, the temperature culturing room that is 24～26 ℃, cultivate 5 weeks, the induced bundle generation of sprouting, as shown in Figure 2;
S4, culture of rootage, indefinite bud on the cutting Multiple Buds, be inoculated on root media, as shown in Figure 3, root media is 1/2MS+NAA0.2mg/L, and postvaccinal culture materials is placed in to intensity of illumination is that 2000～3000LX, light application time are to cultivate in 16h/d, the temperature culturing room that is 24～26 ℃, cultivates 3 weeks, induce root to produce, as shown in Figure 4.
Claims (1)
1. Acer palmatum Green Dragon tissue culture propagation technique, it is characterized in that: it comprises the following steps:
S1, gather disease-free Acer palmatum Green Dragon current-year branch, remove unnecessary blade, be cut into stem with bud, stem with bud is first used alcohol sterilizing 30～60s, then uses mercuric chloride sterilizing 3～5min, then uses sterile water wash 4～6 times;
S2, stem with bud after sterilizing is inoculated on medium and grows, described medium is MS+NAA 0.3mg/L, and postvaccinal culture materials is placed in to intensity of illumination is that 2000～3000LX, light application time are to cultivate in 16h/d, the temperature culturing room that is 24～26 ℃, cultivate 2～3 weeks, induce axillary bud sprouting;
S3, shoot proliferation are cultivated, the axillalry bud of having sprouted is cut, be seeded on subculture medium, subculture medium is MS+6-BA 1.5mg/L+NAA0.3mg/L, and postvaccinal culture materials is placed in to intensity of illumination is that 2000～3000LX, light application time are to cultivate in 16h/d, the temperature culturing room that is 24～26 ℃, cultivate the induced bundle generation of sprouting 3～5 weeks;
S4, culture of rootage, indefinite bud on the cutting Multiple Buds, be inoculated on root media, root media is 1/2MS+NAA0.2mg/L, and postvaccinal culture materials is placed in to intensity of illumination is that 2000～3000LX, light application time are to cultivate in 16h/d, the temperature culturing room that is 24～26 ℃, cultivate 2～3 weeks, induce root to produce.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310402032.XA CN103477981B (en) | 2013-09-06 | 2013-09-06 | Acer palmatum Green Dragon tissue culture propagation technique |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310402032.XA CN103477981B (en) | 2013-09-06 | 2013-09-06 | Acer palmatum Green Dragon tissue culture propagation technique |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN103477981A true CN103477981A (en) | 2014-01-01 |
| CN103477981B CN103477981B (en) | 2015-10-28 |
Family
ID=49818853
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201310402032.XA Active CN103477981B (en) | 2013-09-06 | 2013-09-06 | Acer palmatum Green Dragon tissue culture propagation technique |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN103477981B (en) |
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104285818A (en) * | 2014-10-30 | 2015-01-21 | 江苏省农业科学院 | Tissue culture rapid propagation method of acer palmatum |
| CN104663435A (en) * | 2015-02-09 | 2015-06-03 | 巴中七彩林业科技有限公司 | Method for tissue culture and rapid propagation of acer palmatum hogyoku |
| CN104663436A (en) * | 2015-02-09 | 2015-06-03 | 巴中七彩林业科技有限公司 | Method for tissue culture and rapid propagation of acer palmatum beni kagami |
| CN105409782A (en) * | 2015-12-30 | 2016-03-23 | 四川禾木本业农林科技有限公司 | Tissue culture fast propagation method of acer palmatum 'Ki-hachijo' |
| CN105638460A (en) * | 2015-12-30 | 2016-06-08 | 四川禾木本业农林科技有限公司 | Tissue-culture rapid propagation method for Acer palmatum Butterfly |
| CN109662032A (en) * | 2019-03-05 | 2019-04-23 | 延边大学 | A kind of culture medium and method of acer pseudo-sieboldianum tissue cultures |
| CN111011220A (en) * | 2020-01-10 | 2020-04-17 | 江苏农林职业技术学院 | Tissue culture rapid propagation method of beautiful maple |
| CN112544441A (en) * | 2020-11-10 | 2021-03-26 | 江苏省农业科学院 | Method for establishing tissue culture regeneration system of new acer palmatum variety |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101578963A (en) * | 2009-06-10 | 2009-11-18 | 重庆科技学院 | Tissue culture rapid propagation method for Japanese red maple |
| CN101846506A (en) * | 2010-05-07 | 2010-09-29 | 浙江大学 | Roll angle measurement method based on common path parallel beams |
| CN102668858A (en) * | 2012-06-05 | 2012-09-19 | 江苏省林业科学研究院 | Seedling cultivating method for cutting hard woods of acer palmatum |
| US20130185834P1 (en) * | 2012-01-14 | 2013-07-18 | James David Cavett | Acer rubrum named 'JSC Kingstwo' |
-
2013
- 2013-09-06 CN CN201310402032.XA patent/CN103477981B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101578963A (en) * | 2009-06-10 | 2009-11-18 | 重庆科技学院 | Tissue culture rapid propagation method for Japanese red maple |
| CN101846506A (en) * | 2010-05-07 | 2010-09-29 | 浙江大学 | Roll angle measurement method based on common path parallel beams |
| US20130185834P1 (en) * | 2012-01-14 | 2013-07-18 | James David Cavett | Acer rubrum named 'JSC Kingstwo' |
| CN102668858A (en) * | 2012-06-05 | 2012-09-19 | 江苏省林业科学研究院 | Seedling cultivating method for cutting hard woods of acer palmatum |
Non-Patent Citations (2)
| Title |
|---|
| 李倩中等: "鸡爪槭新品种金陵黄枫组培技术初探", 《江苏农业科学》, vol. 39, no. 4, 31 December 2011 (2011-12-31) * |
| 杨玲等: "色木槭芽直接增殖途径的培养条件", 《林业科技开发》, vol. 26, no. 5, 31 December 2012 (2012-12-31), pages 67 * |
Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104285818A (en) * | 2014-10-30 | 2015-01-21 | 江苏省农业科学院 | Tissue culture rapid propagation method of acer palmatum |
| CN104663435A (en) * | 2015-02-09 | 2015-06-03 | 巴中七彩林业科技有限公司 | Method for tissue culture and rapid propagation of acer palmatum hogyoku |
| CN104663436A (en) * | 2015-02-09 | 2015-06-03 | 巴中七彩林业科技有限公司 | Method for tissue culture and rapid propagation of acer palmatum beni kagami |
| CN104663436B (en) * | 2015-02-09 | 2016-06-15 | 巴中七彩林业科技有限公司 | A kind of tissue culture and rapid propagation method of red mirror |
| CN105409782A (en) * | 2015-12-30 | 2016-03-23 | 四川禾木本业农林科技有限公司 | Tissue culture fast propagation method of acer palmatum 'Ki-hachijo' |
| CN105638460A (en) * | 2015-12-30 | 2016-06-08 | 四川禾木本业农林科技有限公司 | Tissue-culture rapid propagation method for Acer palmatum Butterfly |
| CN105409782B (en) * | 2015-12-30 | 2017-10-20 | 四川七彩林业开发有限公司 | A kind of yellow eight zhang tissue culture and rapid propagation method of mountain red autumnal leaves |
| CN109662032A (en) * | 2019-03-05 | 2019-04-23 | 延边大学 | A kind of culture medium and method of acer pseudo-sieboldianum tissue cultures |
| CN111011220A (en) * | 2020-01-10 | 2020-04-17 | 江苏农林职业技术学院 | Tissue culture rapid propagation method of beautiful maple |
| CN112544441A (en) * | 2020-11-10 | 2021-03-26 | 江苏省农业科学院 | Method for establishing tissue culture regeneration system of new acer palmatum variety |
Also Published As
| Publication number | Publication date |
|---|---|
| CN103477981B (en) | 2015-10-28 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN103477981B (en) | Acer palmatum Green Dragon tissue culture propagation technique | |
| CN103477983B (en) | Acer palmatum red maple tissue culture propagation technique | |
| CN103493729B (en) | Simple tissue culture propagation technology for acerpalmatumseiryu | |
| CN103348920B (en) | Rapid propagation method for high quality seedlings of Kyara | |
| CN103548694B (en) | Tissue culture and rapid propagation method for dracaena cochinchinensis | |
| CN104285813A (en) | Camellia chrysantha tissue culture propagation method | |
| CN103598101A (en) | Dendrobium aphyllum tissue-culture quick propagation method | |
| CN104206270A (en) | Tissue culture and rhizome expansion method of Curcuma hybrida 'Red Torch' | |
| CN105850747B (en) | A kind of jade of the tissue rapid propagation method of the jade of succulent rainbow and its rainbow of culture | |
| CN103461129B (en) | Tissue culture and intermediate propagation method of chirita anachoreta | |
| CN101965796B (en) | Method for performing tissue culture and rapid propagation on primula saxatilis | |
| CN103004604B (en) | Breeding method for vanda | |
| CN104663447A (en) | Building method of orostachys malacophyllus tissue culture regeneration system | |
| CN102939902A (en) | Rapid propagation method of amorphophallus bulbifer through tissue culture | |
| CN106489738B (en) | A kind of production method of spindle tree leaf regeneration plant | |
| CN101836586B (en) | Zhugen ginger detoxification tissue culture seedling industrialized breeding method | |
| CN103477982B (en) | Simple acer palmatum sangokaku tissue culture propagation technique | |
| CN104488723A (en) | Tissue-culture and rapid-propagation method of epimedium koreanum nakai | |
| CN105191792A (en) | Intermediate propagation method of vernonia amygdalina | |
| CN105409768B (en) | The method of floral leaf galingal callus Regeneration System | |
| CN105191795B (en) | A kind of gold leaf metasequoia tissue culture and rapid propagation method | |
| CN102771392B (en) | Tissue culture and rapid propagation process of Chinese Pistache | |
| CN102960248B (en) | Melaleuca bracteata tissue culture rapid propagation process | |
| CN106857255B (en) | A kind of culture medium of dragon fruit tissue cultures | |
| CN102511390B (en) | Method and special culture medium for regenerating sterile induced plants of ormosia fordiana seeds |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| ASS | Succession or assignment of patent right |
Owner name: SICHUAN SHENGZEYUAN AGRICULTURAL INVESTMENT CO., L Free format text: FORMER OWNER: BAZHONG GUANGWUSHAN PLANT RESEARCH INSTITUTE Effective date: 20140123 |
|
| C41 | Transfer of patent application or patent right or utility model | ||
| COR | Change of bibliographic data |
Free format text: CORRECT: ADDRESS; FROM: 636600 BAZHONG, SICHUAN PROVINCE TO: 610041 CHENGDU, SICHUAN PROVINCE |
|
| TA01 | Transfer of patent application right |
Effective date of registration: 20140123 Address after: 610041 B 12F, Air China Century Center, No. 1, air route, Sichuan, Chengdu, Wuhou District Applicant after: VIMA MANUFACTURE ASSOCIATION Address before: The 6 group of Dong Yu Zhen Huai Shu Cun, Bazhong City, Sichuan province Nanjiang County 636600 Applicant before: Bazhong City Guangwushan Institute of Botany |
|
| ASS | Succession or assignment of patent right |
Owner name: BAZHONG QICAI FORESTRY TECHNOLOGY CO., LTD. Free format text: FORMER OWNER: SICHUAN SHENGZEYUAN AGRICULTURAL INVESTMENT CO., LTD. Effective date: 20140709 |
|
| C41 | Transfer of patent application or patent right or utility model | ||
| COR | Change of bibliographic data |
Free format text: CORRECT: ADDRESS; FROM: 610041 CHENGDU, SICHUAN PROVINCE TO: 636007 BAZHONG, SICHUAN PROVINCE |
|
| TA01 | Transfer of patent application right |
Effective date of registration: 20140709 Address after: 636007. 3 to 2, Jia Jie, Jiangbei section, Bazhou Town, Bazhou District, Sichuan, Bazhong Applicant after: BAZHONG COLORFUL FORESTRY TECHNOLOGY CO., LTD. Address before: 610041 B 12F, Air China Century Center, No. 1, air route, Sichuan, Chengdu, Wuhou District Applicant before: VIMA MANUFACTURE ASSOCIATION |
|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C41 | Transfer of patent application or patent right or utility model | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20160122 Address after: Six agency 636611 Dong Yu Zhen Huai Shu Cun, Bazhong city of Sichuan province Nanjiang County Patentee after: SICHUAN QICAI FORESTRY INDUSTRY DEVELOPMENT CO., LTD. Address before: 636007. 3 to 2, Jia Jie, Jiangbei section, Bazhou Town, Bazhou District, Sichuan, Bazhong Patentee before: BAZHONG COLORFUL FORESTRY TECHNOLOGY CO., LTD. |
|
| CP01 | Change in the name or title of a patent holder | ||
| CP01 | Change in the name or title of a patent holder |
Address after: 636611 six Huai Shu Village, Dongyu Town, Nanjiang County, Bazhong, Sichuan Patentee after: Sichuan Qicai Forestry Co., Ltd. Address before: 636611 six Huai Shu Village, Dongyu Town, Nanjiang County, Bazhong, Sichuan Patentee before: SICHUAN QICAI FORESTRY INDUSTRY DEVELOPMENT CO., LTD. |
|
| CP02 | Change in the address of a patent holder | ||
| CP02 | Change in the address of a patent holder |
Address after: 635600 Tissue Culture Workshop Building of Rare and Colorful Leaf Plant Varieties in Guangwu Mountain, Sanshe, Changtan Village, Zhengzheng Town, Nanjiang County, Bazhong City, Sichuan Province Patentee after: Sichuan Qicai Forestry Co., Ltd. Address before: 636611 six Huai Shu Village, Dongyu Town, Nanjiang County, Bazhong, Sichuan Patentee before: Sichuan Qicai Forestry Co., Ltd. |