CN103472238A - Bovine cell factor electrochemical label-free impedimetric immunodetection method - Google Patents
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Abstract
The invention relates to a bovine cell factor electrochemical label-free impedimetric immunodetection method belonging to the technical field of electrochemical immunoassay. The method comprises the following steps: ultrasonically dispersing a nano material in a chitosan solution, thus preparing a nano material/chitosan compound; modifying a bovine cell factor antibody fixed on a glassy carbon electrode with the nano compound, thus obtaining a novel bovine cell factor immunosensor; and then, using the immunosensor for bovine cell factor electrochemical label-free impedimetric immunodetection. The detection method does not need labels, is simple, quick, low in cost, high in sensitivity and favorable in reproducibility and stability, and can be used for early diagnosis of bovine tuberculosis and research on bovine cell immune mechanism.
Description
Technical field
The invention belongs to the electrochemical immunoanalytical technical field, particularly the technical field of immunoassay of ox cell factor.
Background technology
Tuberculosis is that milk cow health and galactopoiesis quality are affected to one of the most serious communicate illness.Gamma interferon (IFN-γ) is under specific inducer spread effect, by a class of T emiocytosis, has the bioactive glycoprotein such as antiviral, antitumor and immunoloregulation function, is to find the earliest, pay close attention to maximum cell factors.Interleukin 4 (IL-4) was found the eighties in 20th century, was a kind of pleiotropism T cell activation factor by the emiocytosis of Th2 type, was the important regulatory factor of organism humoral immunoresponse(HI).Early stage at tuberculosis infection, in the ox body, IFN-γ and IL-4 secretion level significantly improve.If the ox immunological memory cell with during Much's bacillus specific antigen stimulation latent infection, also can produce high-caliber IFN-γ and IL-4.Therefore, ox IFN-γ and IL-4 can be used as the detection index of the early diagnosis of mycobacterium tuberculosis var bovis infection, significant to the control of perlsucht.
At present, biological analysis is ox IFN-γ and IL-4 detection method commonly used, and these biological analysis method sensitivity are low, effort, consuming time and be difficult to standardization.In addition, they,, to detecting buffering agent and other cell factor sensitivity in sample, easily are interfered.Though Enzyme Linked Immunoadsorbent Assay also is established to detect ox IFN-γ and IL-4, but the same complex operation of these methods, effort, consuming time, and sensitivity can not be satisfactory.Due to ox IEN-γ and IL-4 molecular weight and the amount in body body fluid few, also have larger gap so measure at present the distance of sensitivity clinical practice application of the method for ox IEN-γ and IL-4.Therefore, current urgent need is set up a kind of hypersensitivity, fast, method detects IFN-γ, the IL-4 of ox reliably, for the early diagnosis of perlsucht provides a platform.
In recent years, the electrochemical impedance immunoassay is due to it without mark, and highly sensitive, specificity is good, and the characteristics such as the sample consumption is little, Simple fast, become a kind of extremely competitive in-situ check and test method.Nano material has the nano material of unique chemistry, physical and mechanical property, for electrochemical immunoanalytical research provides the recent studies on approach.Therefore, utilize fixedly ox cytokine antibodies of nano material, set up unmarked group of anti-type immunoassay of a galvanochemistry and detect the ox cell factor, significant to the early diagnosis of perlsucht.
Summary of the invention
The object of the invention is to overcome the defect of above-mentioned ox cytokines measurement method, set up the unmarked impedance type immunologic detection method of a kind of ox cell factor galvanochemistry.
Technical scheme of the present invention comprises the following steps;
1) be dispersed in chitosan-acetic acid solution nano material is ultrasonic, form the compound of nano material and shitosan, and then sneak into the ox cytokine antibodies, form nano material/shitosan/ox cytokine antibodies mixed liquor; Described nano material is reduced graphene, mesoporous silica nano-particle or bar-shaped titania;
2) nano material/shitosan/ox cytokine antibodies mixing drop is applied to clean glass-carbon electrode surface, after drying, then, after bovine serum albumin sealing avtive spot, makes the glass-carbon electrode material with ox cell factor immunosensor;
The glass-carbon electrode material that 3) will have an ox cell factor immunosensor is placed in the ox cell factor sample system that contains 37 ℃, and incubation rinsed with aquae destillata after 40 minutes, made ox cell factor immunosensor;
4) the ox cell factor immunosensor made is placed in to the known damping fluid that contains the ox cell factor, under 37 ℃ of conditions, after incubation 40 min, the ox cell factor immunosensor after the taking-up incubation, with distilled water flushing;
The ox cell factor immunosensor of 5) usining after distilled water flushing as working electrode, saturated calomel electrode as auxiliary electrode, platinum plate electrode as to electrode, carry out the AC impedance experiment, make the AC impedance spectrogram of this ox cell factor of same ox cell factor and variable concentrations;
6) obtain the diameter of each spectrogram from each AC impedance spectrogram, then make the logarithm value of ox cytokine concentration and impedance front and back relative changing value's linear relationship chart;
7) ox cell factor immunosensor is placed in to the damping fluid of testing sample, under 37 ℃ of conditions, after incubation 40 min, the ox cell factor immunosensor after the taking-up incubation, with distilled water flushing; Then carry out the AC impedance experiment with the condition identical with step 5), obtain the AC impedance spectrogram of ox cell factor, then read the diameter value of spectrogram;
8) in the linear relationship chart of obtaining in step 6), finding step 7) the corresponding value of diameter value of the spectrogram obtained, the i.e. logarithm value of the ox cytokine concentration of testing sample.
Above step 1) to 6) be to make the logarithm value of ox cytokine concentration and impedance front and back relative changing value's linear relationship chart, step 7) is that relative changing value before and after the impedance of testing sample is detected, and can directly obtain the logarithm value of the ox cytokine concentration of testing sample by linear relationship chart.The present invention be take shitosan as making the film forming agent of sensor, and utilization has the nano material that specific surface area is larger and fixes the ox cytokine antibodies, to improve analytical effect.This immunosensor can be used for the quantitative detection of the cell factors such as Bov IFN-γ, interleukin-4.
The present invention has the following advantages:
(1) this analytical approach without any mark, simple, fast, low, highly sensitive, the reappearance of cost and good stability, can be for the early diagnosis of perlsucht and the research of ox cellular immunity mechanism.
(2) nano material large, the good biocompatibility that has specific surface area, utilize its fixed cell factor antibody, be conducive to keep the biologically active of antibody, widen the range of linearity of detection, improve immunity and answer specificity and reaction efficiency, reduce non-specific adsorption, improve detection sensitivity.
(3) shitosan is the deacetylation product of natural polymers chitin, and it has good film forming and tenability, and good biocompatibility, is usually used in the fixedly matrix of biomaterial, shows significant physiologically active and biodegradability.
In addition, the present invention by nano material ultrasonic be dispersed in chitosan-acetic acid solution in the time, the chitosan mass number percent in described chitosan-acetic acid solution is 1%, the rate of charge of described nano material and chitosan-acetic acid solution is 2mg:1mL.Can make nano material be dispersed in chitosan-acetic acid solution uniformly, so that better sessile antibody.
Concrete grammar with bovine serum albumin sealing avtive spot is: the glass-carbon electrode after drying is inserted in the bovine serum albumin of normal temperature, is placed in 37 ℃ of water-bath 30 min.
In order to get rid of the impact of electrode surface impurity on measuring, before described painting nano material/shitosan/ox cytokine antibodies mixed liquor, alumina powder polishing by glass-carbon electrode with 0.05 mm, rinse out residual alumina powder with deionized water again, then put into the dilute nitric acid solution ultrasonic cleaning, finally use successively ethanol and redistilled water cleaning electrode surface, obtain clean glass-carbon electrode.
Described dilute nitric acid solution is to take by nitric acid and water the salpeter solution that ratio that volume ratio is 1:1 is mixed to form.Because nitric acid has strong oxidizing property, can remove the impurity of electrode surface stubbornness.
The potassium ferricyanide that the test solution of described AC impedance experiment is 1:1 by volume ratio and potpourri 5 mM, 0.1 M phosphate buffered solution and the 0.1 M Klorvess Liquid of potassium ferrocyanide form.
The accompanying drawing explanation
Fig. 1 joins by the ox IFN-γ immunosensor of preparation the AC impedance spectrogram obtained after variable concentrations ox IFN-γ in embodiment 1.
The logarithm value that Fig. 2 is the ox IFN-γ concentration that makes from Fig. 1 and impedance front and back relative changing value's linear relationship chart.
Embodiment
Below experimentation of the present invention is described in detail, is intended to make design cycle of the present invention, purpose of design and innovative point thereof and advantage clearer.
Embodiment 1:
One, make the logarithm value of ox IFN-γ concentration and impedance front and back relative changing value's linear relationship chart:
(1) adopt chemical dispersion method to prepare the reduced graphene nano material: to get graphite oxide 0.1g and mix with 100mL distilled water, sonic oscillation 30 min.By vibrating dispersion, good graphite oxide joins in three-necked bottle afterwards, and adds the 1g sodium borohydride, adds hot reflux 8h under 100 ℃.Standing filtration, dry the reduced graphene that is of rear collection generation naturally.
(2) prepare the mixed liquor of reduced graphene/shitosan/ox IFN-gamma antibodies: in the chitosan-acetic acid solution that is 1% by the chitosan mass number percent of the ultrasonic 1mL of being scattered in of 2mg reduced graphene, form the compound of reduced graphene/shitosan.
Then add ox IFN-gamma antibodies 400 μ g/mL in above compound, be uniformly mixed, form the mixed liquor of reduced graphene/shitosan/ox IFN-gamma antibodies.
(3) clean glass-carbon electrode material: the alumina powder polishing by glass-carbon electrode with 0.05mm, rinse out residual alumina powder with deionized water, put into the dilute nitric acid solution ultrasonic cleaning, finally use successively ethanol and redistilled water cleaning electrode surface.
(4) get reduced graphene/shitosan/ox IFN-gamma antibodies mixing drop and be applied to clean glass-carbon electrode surface, after drying, puts at electrode the bovine serum albumin of 10.0 mg/mL, be placed in 37 ℃ of water-bath 30 min, to seal non-specific site, make ox IFN-γ immunosensor.
(5) the ox IFN-γ immunosensor made is placed in respectively to the ox IFN-γ buffer solution of the different concentration be diluted to by buffer solution, then, respectively at 37 ℃ of incubation 40 min, after distilled water flushing, detects respectively its electrochemical impedance signal.
(6) the AC impedance experiment is carried out on the Autolab electrochemical workstation, and the frequency range of Based on AC Impedance Measurements is 10
-1hz to 10
5hz, used open circuit potential, and the amplitude of sinusoidal wave current potential is 10 mV, 0.1 M phosphate buffered solution and the 0.1 M Klorvess Liquid of testing impedance liquid for containing the 5 mM potassium ferricyanide/potassium ferrocyanides (volume ratio is that 1:1 is mixed).
Each ox IFN-γ immunosensor of usining after distilled water flushing as working electrode, saturated calomel electrode as auxiliary electrode, platinum plate electrode as to electrode, carry out respectively the AC impedance experiment, make the AC impedance spectrogram of the ox IFN-γ of ox IFN-γ immunosensor and variable concentrations, as shown in Figure 1.In Fig. 1, Zre is real axis, and Zim is the imaginary axis.
(7) obtain the diameter of each spectrogram from each AC impedance spectrogram, then make the logarithm value of ox IFN-γ concentration and impedance front and back relative changing value's linear relationship chart, as shown in Figure 2.LogC in Fig. 2
boIFN-γfor the logarithmic graph of Bov IFN-γ concentration, Δ R
ct(%) be relative changing value before and after impedance.
Two, sample is carried out the logarithm value detection of ox IFN-γ concentration:
(1) the ox IFN-γ immunosensor of above step (4) being made is placed in the damping fluid of testing sample, and under 37 ℃ of conditions, after incubation 40 min, the ox IFN-γ immunosensor after the taking-up incubation, with distilled water flushing.
(2) carry out the AC impedance experiment with the condition identical with above step (6), obtain the AC impedance spectrogram of ox IFN-γ, then obtain the diameter value of spectrogram.
(3) search the corresponding value of diameter value of the spectrogram of testing sample in the linear relationship chart of obtaining in step (7), i.e. the logarithm value of the ox IFN-γ concentration of testing sample.
Embodiment 2:
One, make the logarithm value of ox IL-4 concentration and impedance front and back relative changing value's linear relationship chart:
(1) bar-shaped titania is synthetic: under the ice-water bath condition, the titanium tetrachloride of 1.8 mL is slowly joined in the 50 mL beakers that fill 19 mL distilled water, vigorous stirring 10 min, obtain white suspension.15 mL chloroform solvents are joined in this suspension, and magnetic agitation 10 min, transfer in the teflon reactor, is placed in the baking oven of 160 ℃, hydro-thermal reaction 12 h.Use the absolute ethyl alcohol centrifuge washing to neutrality products therefrom, be placed in 60 ℃ of vacuum tanks and dry 24 h, under room temperature, grind and obtain product.
(2) prepare the mixed liquor of titania/shitosan/ox IL-4 antibody: by the ultrasonic 1 % chitosan-acetic acid solution that is scattered in 1 mL of 2 mg titanium dioxide nano-rods, then add ox IL-4 antibody, be uniformly mixed, form the mixed liquor of titania/shitosan/ox IL-4 antibody.
(3) clean glass-carbon electrode material: the alumina powder polishing by glass-carbon electrode with 0.05 mm, rinse out residual alumina powder with deionized water, put into the dilute nitric acid solution ultrasonic cleaning, finally use successively ethanol and redistilled water cleaning electrode surface.
(4) get titania/shitosan/ox IL-4 antibody mixing drop and be applied to clean glass-carbon electrode surface, put into the bovine serum albumin of 10.0 mg/mL after drying, be placed in 37 ℃ of water-bath 30 min, to seal non-specific site, make ox IFN-γ immunosensor.
(5) the ox IL-4 immunosensor made is placed in respectively to the ox IL-4 buffer solution of the different concentration be diluted to by buffer solution, then, respectively at 37 ℃ of incubation 40 min, after distilled water flushing, detects respectively its electrochemical impedance signal.
(6) the AC impedance experiment is carried out on the Autolab electrochemical workstation, and the frequency range of Based on AC Impedance Measurements is 10
-1hz to 10
5hz, used open circuit potential, and the amplitude of sinusoidal wave current potential is 10 mV, 0.1 M phosphate buffered solution and the 0.1 M Klorvess Liquid of testing impedance liquid for containing the 5 mM potassium ferricyanide/potassium ferrocyanides (1:1).
Each ox IL-4 immunosensor of usining after distilled water flushing as working electrode, saturated calomel electrode as auxiliary electrode, platinum plate electrode as to electrode, carry out respectively the AC impedance experiment, make the AC impedance spectrogram of the ox IL-4 of ox IL-4 immunosensor and variable concentrations.
(7) obtain the diameter of each spectrogram from each AC impedance spectrogram, then make the logarithm value of ox IL-4 concentration and impedance front and back relative changing value's linear relationship chart.
Two, sample is carried out the logarithm value detection of ox IL-4 concentration:
(1) the ox IL-4 immunosensor of above step (4) being made is placed in the damping fluid of testing sample, and under 37 ℃ of conditions, after incubation 40 min, the ox IL-4 immunosensor after the taking-up incubation, with distilled water flushing.
(2) carry out the AC impedance experiment with the condition identical with above step (6), obtain the AC impedance spectrogram of ox IL-4, then obtain the diameter value of spectrogram.
(3) search the corresponding value of diameter value of the spectrogram of testing sample in the linear relationship chart of obtaining in step (7), i.e. the logarithm value of the ox IL-4 concentration of testing sample.
Embodiment 3:
One, make the logarithm value of ox IL-4 concentration and impedance front and back relative changing value's linear relationship chart:
(1) mesoporous silica nano-particle is synthetic: take the 10g cetyl trimethyl ammonium bromide and be dissolved in 480 mL distilled water, and add sodium hydroxide solution (2.00 M, 3.50 mL), keep solution temperature at 80 ℃.5 mL triethoxysilanes are dropwise added in this solution, and stir 2 h under 80 ℃ of conditions, the white depositions obtained method is after filtration separated.The silica dioxide granule that separation is obtained is calcined 5 h and is obtained final product in 550 ℃ of muffle furnaces.
(2) prepare the mixed liquor of silicon dioxide/shitosan/ox IFN-gamma antibodies: by the ultrasonic 1 % chitosan-acetic acid solution that is scattered in 1 mL of 2 mg mesoporous silicon oxides, then add ox IFN-gamma antibodies, be uniformly mixed, form the mixed liquor of silicon dioxide/shitosan/ox IFN-gamma antibodies.
(3) clean glass-carbon electrode material: the alumina powder polishing by glass-carbon electrode with 0.05 mm, rinse out residual alumina powder with deionized water, put into the dilute nitric acid solution ultrasonic cleaning, finally use successively ethanol and redistilled water cleaning electrode surface.
(4) get silicon dioxide/shitosan/ox IFN-gamma antibodies mixing drop and be applied to clean glass-carbon electrode surface, put into the bovine serum albumin of 10.0 mg/mL after drying, be placed in 37 ℃ of water-bath 30 min, to seal non-specific site, make ox IFN-γ immunosensor.
(5) the ox IFN-γ immunosensor made is placed in respectively to the ox IFN-γ buffer solution of the different concentration be diluted to by buffer solution, then, respectively at 37 ℃ of incubation 40 min, after distilled water flushing, detects respectively its electrochemical impedance signal.
(6) the AC impedance experiment is carried out on the Autolab electrochemical workstation, and the frequency range of Based on AC Impedance Measurements is 10
-1hz to 10
5hz, used open circuit potential, and the amplitude of sinusoidal wave current potential is 10 mV, 0.1 M phosphate buffered solution and the 0.1 M Klorvess Liquid of testing impedance liquid for containing the 5 mM potassium ferricyanide/potassium ferrocyanides (1:1).
Each ox IFN-γ immunosensor of usining after distilled water flushing as working electrode, saturated calomel electrode as auxiliary electrode, platinum plate electrode as to electrode, carry out respectively the AC impedance experiment, make the AC impedance spectrogram of the ox IL-4 of ox IFN-γ immunosensor and variable concentrations.
(7) obtain the diameter of each spectrogram from each AC impedance spectrogram, then make the logarithm value of ox IFN-γ concentration and impedance front and back relative changing value's linear relationship chart.
Two, sample is carried out the logarithm value detection of ox IFN-γ concentration:
(1) the ox IFN-γ immunosensor of above step (4) being made is placed in the damping fluid of testing sample, and under 37 ℃ of conditions, after incubation 40 min, the ox IFN-γ immunosensor after the taking-up incubation, with distilled water flushing.
(2) carry out the AC impedance experiment with the condition identical with above step (6), obtain the AC impedance spectrogram of ox IFN-γ, then obtain the diameter value of spectrogram.
(3) search the corresponding value of diameter value of the spectrogram of testing sample in the linear relationship chart of obtaining in step (7), i.e. the logarithm value of the ox IFN-γ concentration of testing sample.
Claims (6)
1. the unmarked impedance type immunologic detection method of ox cell factor galvanochemistry, is characterized in that comprising the following steps;
1) be dispersed in chitosan-acetic acid solution nano material is ultrasonic, form the compound of nano material and shitosan, and then sneak into the ox cytokine antibodies, form nano material/shitosan/ox cytokine antibodies mixed liquor; Described nano material is reduced graphene, mesoporous silica nano-particle or bar-shaped titania;
2) nano material/shitosan/ox cytokine antibodies mixing drop is applied to clean glass-carbon electrode surface, after drying, then, after bovine serum albumin sealing avtive spot, makes the glass-carbon electrode material with ox cell factor immunosensor;
The glass-carbon electrode material that 3) will have an ox cell factor immunosensor is placed in the ox cell factor sample system that contains 37 ℃, and incubation rinsed with aquae destillata after 40 minutes, made ox cell factor immunosensor;
4) the ox cell factor immunosensor made is placed in to the known damping fluid that contains the ox cell factor, under 37 ℃ of conditions, after incubation 40 min, the ox cell factor immunosensor after the taking-up incubation, with distilled water flushing;
The ox cell factor immunosensor of 5) usining after distilled water flushing as working electrode, saturated calomel electrode as auxiliary electrode, platinum plate electrode as to electrode, carry out the AC impedance experiment, make the AC impedance spectrogram of this ox cell factor of same ox cell factor and variable concentrations;
6) obtain the diameter of each spectrogram from each AC impedance spectrogram, then make the logarithm value of ox cytokine concentration and impedance front and back relative changing value's linear relationship chart;
7) ox cell factor immunosensor is placed in to the damping fluid of testing sample, under 37 ℃ of conditions, after incubation 40 min, the ox cell factor immunosensor after the taking-up incubation, with distilled water flushing; Then carry out the AC impedance experiment with the condition identical with step 5), obtain the AC impedance spectrogram of ox cell factor, then read the diameter value of spectrogram;
8) in the linear relationship chart of obtaining in step 6), finding step 7) the corresponding value of diameter value of the spectrogram obtained, the i.e. logarithm value of the ox cytokine concentration of testing sample.
2. according to the unmarked impedance type immunologic detection method of the described a kind of ox cell factor galvanochemistry of claim 1, it is characterized in that by nano material ultrasonic be dispersed in chitosan-acetic acid solution in the time, chitosan mass number percent in described chitosan-acetic acid solution is 1%, and the rate of charge of described nano material and chitosan-acetic acid solution is 2mg:1mL.
3. according to the unmarked impedance type immunologic detection method of the described a kind of ox cell factor galvanochemistry of claim 1, it is characterized in that with the concrete grammar of bovine serum albumin sealing avtive spot being: the glass-carbon electrode after drying is inserted in the bovine serum albumin of normal temperature, is placed in 37 ℃ of water-bath 30 min.
4. according to the unmarked impedance type immunologic detection method of the described a kind of ox cell factor galvanochemistry of claim 1, it is characterized in that before described painting nano material/shitosan/ox cytokine antibodies mixed liquor, alumina powder polishing by glass-carbon electrode with 0.05 mm, rinse out residual alumina powder with deionized water again, then put into the dilute nitric acid solution ultrasonic cleaning, finally use successively ethanol and redistilled water cleaning electrode surface, obtain clean glass-carbon electrode.
5. the unmarked impedance type immunologic detection method of a kind of ox cell factor galvanochemistry according to claim 4, is characterized in that described dilute nitric acid solution is to take by nitric acid and water the salpeter solution that ratio that volume ratio is 1:1 is mixed to form.
6. the unmarked impedance type immunologic detection method of a kind of ox cell factor galvanochemistry according to claim 4, the potassium ferricyanide that the test solution that it is characterized in that described AC impedance experiment is 1:1 by volume ratio and potpourri 5 mM, 0.1 M phosphate buffered solution and the 0.1 M Klorvess Liquid of potassium ferrocyanide form.
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104330553A (en) * | 2014-11-20 | 2015-02-04 | 扬州大学 | Unmarked chemiluminescent immunosensor and immunoassay method thereof |
| CN109475669A (en) * | 2016-05-24 | 2019-03-15 | 苏马瓦克医疗解决方案公司 | For collecting the portable device with disposable reservoir of internal liquid after surgery |
| CN110482566A (en) * | 2019-09-02 | 2019-11-22 | 杭州电子科技大学 | A kind of meso titanium silica compound and its application in nucleic acid molecules detection |
| WO2020168607A1 (en) * | 2019-02-20 | 2020-08-27 | 青岛大学 | NANOCOMPOSITE, AND METHOD FOR PREPARING LABEL-FREE APTAMER ELECTROCHEMICAL γ-INTERFERON SENSOR THEREOF |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR100838323B1 (en) * | 2007-01-23 | 2008-06-13 | 부산대학교 산학협력단 | Impedimetric immunosensor for detecting bisphenol a and detecting method using the same |
| CN101957375A (en) * | 2010-10-15 | 2011-01-26 | 扬州大学 | Label-free impedance type immunosensor for furaltadone residues, preparation method and application thereof |
| CN102914576A (en) * | 2012-11-19 | 2013-02-06 | 桂林理工大学 | Preparation method of redox probe for marking CRP (C-Reactive Protein) impedance immunosensor |
-
2013
- 2013-09-26 CN CN201310443715XA patent/CN103472238A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR100838323B1 (en) * | 2007-01-23 | 2008-06-13 | 부산대학교 산학협력단 | Impedimetric immunosensor for detecting bisphenol a and detecting method using the same |
| CN101957375A (en) * | 2010-10-15 | 2011-01-26 | 扬州大学 | Label-free impedance type immunosensor for furaltadone residues, preparation method and application thereof |
| CN102914576A (en) * | 2012-11-19 | 2013-02-06 | 桂林理工大学 | Preparation method of redox probe for marking CRP (C-Reactive Protein) impedance immunosensor |
Non-Patent Citations (4)
| Title |
|---|
| HUIFENG XU,ET AL: "Direct electrochemistry and electrocatalysis of hemoglobin protein entrapped in graphene and chitosan composite film", 《TALANTA》, vol. 81, 31 December 2010 (2010-12-31) * |
| RAJU KHAN AND MARSHAL DHAYAL: "Nanocrystalline bioactive TiO2-chitosan impedimetric immunosensor for ochratoxin-A", 《ELECTROCHEMISTRY COMMUNICATIONS》, vol. 10, 31 December 2008 (2008-12-31) * |
| YI WAN, ET AL: "Impedimetric immunosensor doped with reduced grapheme sheets fabricated by controllable electrodeposition for the non-labelled detection of bacteria", 《BIOSENSORS AND BIOELECTRONICS》, vol. 26, 31 December 2011 (2011-12-31) * |
| 赵洁，等: "基于还原石墨烯的牛白介素-4无标记电化学阻抗免疫传感器", 《第八届全国化学生物学学术会议论文摘要集》, 15 September 2013 (2013-09-15) * |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104330553A (en) * | 2014-11-20 | 2015-02-04 | 扬州大学 | Unmarked chemiluminescent immunosensor and immunoassay method thereof |
| CN109475669A (en) * | 2016-05-24 | 2019-03-15 | 苏马瓦克医疗解决方案公司 | For collecting the portable device with disposable reservoir of internal liquid after surgery |
| CN109475669B (en) * | 2016-05-24 | 2022-01-04 | 苏马瓦克医疗解决方案公司 | Portable device with disposable reservoir for collecting internal fluids after surgery |
| WO2020168607A1 (en) * | 2019-02-20 | 2020-08-27 | 青岛大学 | NANOCOMPOSITE, AND METHOD FOR PREPARING LABEL-FREE APTAMER ELECTROCHEMICAL γ-INTERFERON SENSOR THEREOF |
| US10883984B1 (en) | 2019-02-20 | 2021-01-05 | Qingdao University | Method for preparing nanocomposite and label-free aptamer electrochemical sensor of gamma-interferon based on the nanocomposite |
| CN110482566A (en) * | 2019-09-02 | 2019-11-22 | 杭州电子科技大学 | A kind of meso titanium silica compound and its application in nucleic acid molecules detection |
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Application publication date: 20131225 |