CN103451127A - Zoogloearesiniphila HJ1 with ortho-xylene degradation capacity and application thereof - Google Patents

Zoogloearesiniphila HJ1 with ortho-xylene degradation capacity and application thereof Download PDF

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CN103451127A
CN103451127A CN2013102814122A CN201310281412A CN103451127A CN 103451127 A CN103451127 A CN 103451127A CN 2013102814122 A CN2013102814122 A CN 2013102814122A CN 201310281412 A CN201310281412 A CN 201310281412A CN 103451127 A CN103451127 A CN 103451127A
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xylol
degradation
moving glue
degradation capability
glue bacterium
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CN103451127B (en
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於建明
成卓韦
蒋轶锋
朱润晔
顾信娜
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ZHEJIANG ZHONGYIN ENVIRONMENTAL TECHNOLOGY CO., LTD.
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Zhejiang University of Technology ZJUT
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    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
    • Y02A50/20—Air quality improvement or preservation, e.g. vehicle emission control or emission reduction by using catalytic converters
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P20/00—Technologies relating to chemical industry
    • Y02P20/50—Improvements relating to the production of bulk chemicals
    • Y02P20/59—Biological synthesis; Biological purification

Abstract

The invention relates to Zoogloearesiniphila HJ1 with ortho-xylene degradation capacity and application thereof in an ortho-xylene organic pollutant. The collection number of the Zoogloearesiniphila HJ1 is CCTCC NO. M2012235. The invention also provides application of the Zoogloearesiniphila HJ1 with the ortho-xylene degradation capacity in degrading organic pollutants, namely benzene, methylbenzene, ethylbenzene, dimethylbenzene and chlorobenzene. The Zoogloearesiniphila HJ1 disclosed by the invention can be used for efficiently degrading the benzene series pollutants, such as ortho-xylene, and is high in tolerance capacity.

Description

One strain has moving glue bacterium and the application thereof of o-Xylol degradation capability
Technical field
The invention belongs to environmental pollutant biologic treating technique field, be specifically related to a strain have the o-Xylol degradation capability moving glue bacterium ( zoogloea resiniphila) HJ1 and application thereof.
Background technology
O-Xylol (C 8h 10, o-xylene) be to produce phthalic anhydride (Tetra hydro Phthalic anhydride, PA), the industrial chemicals of dyestuff, agrochemical, wherein 90% left and right is for the production of phthalic anhydride.Its steam and air form explosive mixture, and chance naked light, high heat energy cause combustion explosion.Its steam is heavier than air, and can be diffused in lower place quite far away, meets burning things which may cause a fire disaster and is drawing and strile-back.If meet high heat, press and increase in container, the danger of cracking and blast is arranged.If flow velocity is too fast, easily produce and gather static.Skin, mucous membrane are had to hormesis, central nervous system is had to anesthetic action; Long term can affect liver, renal function.Acute poisoning: patient has the severe persons such as cough, stream are prevented, conjunctival congestion that illusion, delirium, obnubilation etc. are arranged, and the hysteria sample that has had shows effect.O-Xylol is classified as one of 129 kinds of preferential toxic pollutants of controlling by Environmental Protection Agency (EPA).Therefore the purification techniques of studying o-Xylol just seems very urgent and important.
The biopurification technology has the characteristics such as the efficiency of removal is high, processing costs is low, secondary pollution is little, is widely used in gradually degraded and the purification of toxic pollutant.One of key that adopts biotechnology processing o-Xylol is to obtain the bacterial strain with efficient degradation o-Xylol ability.At present, Chinese scholars has been carried out large quantity research to the biological degradation of benzene compounds, the o-Xylol degradation bacteria be separated to so far mainly comprise rhodococcus ( rhodococcus), pseudomonas ( pseudomonas) etc.Large quantity research shows the efficient o-Xylol degradation bacteria of separation screening from environment, remains one of important method of eliminating BETX pollutent in environment.
Moving glue bacterium in the present invention ( zoogloea resiniphila) be a kind of common tyrothricin, in the patent No., be 201010249613.0, the Chinese patent that patent name is " for composite and highly effective microorganism preparation and the synergistic process of synergy of sewage treatment plant " discloses a kind of compound formulation that uses moving glue bacterium, makes from the multiple mushroom of hair Zymomonas mobilis, genus bacillus, pseudomonas, Alcaligenes and Nocardia, for the treatment of various sewage, especially high saliferous and high-ammonia-nitrogen sewage.Through patent searching and other pertinent literatures, not yet find to utilize the report of this strain degradation o-Xylol.The discovery of this degradation bacteria is significant for the high-efficient purification of the benzo pollutants such as BETX in trade effluent waste gas.
Summary of the invention
Technical problem to be solved by this invention is to overcome deficiency of the prior art and a kind of efficient, moving glue bacterium and application thereof with o-Xylol degradation capability that tolerance is strong are provided.
The technical scheme that the present invention solves the problems of the technologies described above employing is: a strain have the o-Xylol degradation capability moving glue bacterium ( zoogloea resiniphila) HJ1, the GenBank number of logging in of bacterial strain 16S rDNA is JQ751310, this bacterial strain is preserved in Chinese Typical Representative thing preservation center, address: and Wuhan, China Wuhan University, 430072, deposit number is: CCTCC NO. M2012235.Being characterized as of this bacterial strain: bacterium colony is white in color, and neat in edge is smooth moistening.The form of observing this thalline under transmission electron microscope is tyrothricin, amphitrichous, Gram-negative, oxidase positive.
The invention provides the application of moving glue bacterium HJ1 in degraded o-Xylol organic pollutant with o-Xylol degradation capability.
The present invention also provides the application of the moving glue bacterium HJ1 with o-Xylol degradation capability in degraded benzene, toluene, ethylbenzene, dimethylbenzene, chlorobenzene organic pollutant.
The present invention puts into the described moving glue bacterium HJ1 with o-Xylol degradation capability in the sewage containing o-Xylol through cultivating the bacteria suspension obtained, and in temperature, is 25-35 ℃, under the condition of pH value for 7-9, cultivates.
Moving glue bacterium HJ1 optimum culturing temperature in waste water with o-Xylol degradation capability of the present invention is 35 ℃, pH7, and the o-Xylol degradation rate is 5.33 mg/ (Lh).
Moving glue bacterium HJ1 degraded o-Xylol concentration range with o-Xylol degradation capability of the present invention is 0-256 mg/L, and the high specific degradation rate is 0.19 mg o-Xylol/(mg cellsh).
Moving glue bacterium HJ1 with o-Xylol degradation capability of the present invention finally is converted into CO to o-Xylol 2, H 2o and cellular biomass, its average yield coefficient is 0.2572 mg cells/mg o-Xylol, average mineralization rate is 54.8%.
The present invention compared with prior art has the following advantages: can realize the high-efficient purification of benzo pollutants, the especially o-Xylol such as BETX in trade effluent waste gas, and not produce any secondary pollution.Easily promote, purify cost low.
The accompanying drawing explanation
Fig. 1 is zoogloea resiniphilathe gramstaining photo of HJ1.
Fig. 2 is zoogloea resiniphilathe transmission electron microscope photo of HJ1.
Fig. 3 is zoogloea resiniphilathe phylogeny tree graph of HJ1.
Fig. 4 is zoogloea resiniphilahJ1 is to cellular biomass change curve in the degradation curve of 32mg/L, 64 mg/L, 96 mg/L, 128 mg/L, 160 mg/L o-Xylols, degradation process.
Fig. 5 is zoogloea resiniphilahJ1 is to cellular biomass change curve in the degradation curve of 192 mg/L, 224 mg/L, 256 mg/L, 288 mg/L o-Xylols, degradation process.
Fig. 6 is zoogloea resiniphilahJ1 is to the matched curve of o-Xylol specific degradation rate.
Fig. 7 is zoogloea resiniphilahJ1 is CO in the process of degraded o-Xylol 2the growing amount matched curve.
Fig. 8 is zoogloea resiniphilahJ1 is the cellular biomass formation curve in the process of degraded o-Xylol.
Fig. 9 is zoogloea resiniphilahJ1 is to the common organic pollutant degradation performance analysis of industry.
Embodiment
The experimental technique used in following embodiment if no special instructions, is ordinary method.
In following embodiment, material used, reagent etc., if no special instructions, all can obtain from commercial channels.
Embodiment mono-, zoogloea resiniphilathe separation of HJ1, purifying and evaluation thereof.
1. zoogloea resiniphilathe separation of HJ1 and purifying.
zoogloea resiniphilahJ1 is the strain Gram-negative bacteria that domestication from the active sludge of Xinchang waste water in pharmaceutical plants treatment station, separation obtain.Concrete steps are as follows:
Get the active sludge of Xinchang waste water in pharmaceutical plants treatment station, after tap water is eluriated five times, the sky 48h that exposes to the sun removes residual organism as far as possible.Preparation inorganic salt nutrient solutions (pH7), the o-Xylol of take is tamed as sole carbon source carries out orientation to active sludge, and 2 d change fresh inorganic salt nutrient solution, measure the pH of nutrient solution every day.Preparation o-Xylol starting point concentration is 100 mg/L minimal mediums, and compound method is as follows: 1g/L KH 2pO 4, 4.5g/L Na 2hPO 4, 0.5g/L (NH 4) 2sO 4, 0.1g/L MgSO 4, 0.02g/L CaCl 2, 0.1mg/L MnSO 44H 2o, 0.1mg/L ZnSO 47H 2o, 0.02mg/L CuSO 45H 2o, 0.02mg/L CoCl 26H 2o, 0.02 mg/L Na 2moO 42H 2o, 1mg/L FeSO 47H 2o, 0.02mg/L H 3bO 3, pH 7.3 ~ 7.55.Be sub-packed in the sealing saline bottle (50 mL/) of 250 mL, 110 ℃ of sterilizing 40 min.After substratum is cooling, add o-Xylol as sole carbon source, the mud of the domestication amount that by volume mark is 5% is joined in substratum, in 30 ℃, the shaking table shaking culture of 160 rpm.About 3d is transferred in the minimal medium after fresh sterilizing with 10% amount, the shaking table shaking culture.In conjunction with agar inorganic salt plate streaking, separate, picking list bacterium colony access minimal medium shaking table continues shaking culture, until obtain single bacterium of the degradable o-Xylol that growth is fast in above-mentioned substratum, bacterium colony is regular and proterties is stable.
2. zoogloea resiniphilathe evaluation of HJ1
By 16S rDNA sequential analysis and Physiology and biochemistry experimental identification, determine that bacterial strain HJ1 is zoogloea resiniphila.Concrete steps are as follows:
Adopt the DNA of the centrifugal environmental sample DNA recovery test kit of 3S post (V2.2, Shanghai Shenergy Biocolor BioScience & Technology Company) extraction and purifying bacterial strain, 4 ℃ of preservations.Select the universal primer BSF8/20 of bacterium and BSR1541/20 to carry out pcr amplification to the DNA of purifying, primer sequence is respectively:
BSF8/20：5'-AGAGT TTGAT CCTGG CTCAG-3'
BSR1541/20：5'-AAGGA GGTGA TCCAG CCGCA-3'
The PCR response procedures is set as: 94 ° of C denaturation 4 min; Then 94 ° of C sex change 1 min, 59 ° of C, 1 min that anneals, 72 ° of C extend 1.5 min, circulate 35 cycles; Then 72 ° of C extend 10 min; Last 4 ° of C keep 10 min.The PCR product is checked order (the prompt base in the English Weihe River, Shanghai), sequencing result is shown in sequence table.
The 16S rDNA sequence of bacterial strain HJ1 is uploaded to Genbank, obtain the accession number JQ751310 of Genbank, carry out homology relatively with the gene order in Genbank simultaneously, find that it belongs to zoogloeabelong to, with zoogloea resiniphilastrain DhA-35(NR027188) homology is the highest, reaches 99%.By this bacterial strain called after zoogloea resiniphilhJ1, and (address: Wuhan, China Wuhan University, postcode 430072) preservation at Chinese Typical Representative culture collection center on June 18th, 2012, preserving number is CCTCC NO.M2012235.
Embodiment bis-, zoogloea resiniphilahJ1 detects the degradation property of different concns o-Xylol.
Under optimum environment factor condition (nutrient solution pH7,35 ℃ of culture temperature), investigated the degradation property of bacterial strain LX-1 to starting point concentration 32-288mg/L o-Xylol.Result shows, the degradable 32-256 mg/L o-Xylol of bacterial strain HJ1 energy, but can only the Partial digestion starting point concentration be 288 mg/L o-Xylols.Under optimal culture condition, bacterial strain high specific degradation rate has reached 0.19 mg o-xylene/(mg cellsh).Concrete implementation step is as follows:
Inorganic salt nutrient solution 1000 mL of preparation pH=7, be sub-packed in the saline bottle of 250mL every bottle of 50mL, 110 ℃ of sterilizing 40 min.After nutrient solution is cooling, get wherein 10 bottles and add the bacteria suspension in the HJ1 of logarithmic phase, add o-Xylol as sole carbon source, make its concentration reach 32,64,128,160,192,224,256,288 mg/L; Blank is set.Saline bottle seals rear 35 ℃ of shaking culture, regularly measures o-Xylol concentration and biomass residual in nutrient solution, draws bacterial strain HJ1 for different starting point concentration o-Xylols degradation curve very, and calculates corresponding specific degradation rate.
Fig. 4 ~ 6 are that bacterial strain HJ1 is with the degradation curve without the starting point concentration o-Xylol.Result shows, when o-Xylol concentration, during lower than 256 mg/L, bacterial strain HJ1 can be degradable; But bacterial strain HJ1 fails to realize degradable to 288 mg/L o-Xylols, and after 132 h, its residual concentration is about 113.5 mg/L.When o-Xylol concentration, during lower than 160 mg/L, the biomass of bacterial strain HJ1 is along with the concentration of o-Xylol raises and increases; When the concentration of o-Xylol is 160-224 mg/L, the biomass of bacterial strain remains unchanged, and maintains 46 mg/L left and right; When the concentration of o-Xylol rises to 256 mg/L, the biomass of bacterial strain drops to 36.6 mg/L; When o-Xylol concentration further rises to 288 mg/L, the biomass of bacterial strain further drops to 15.5 mg/L.Pass through Haldane ' s inhibition growth kinetics model simultaneously specific degradation rate has been carried out to matching, obtained the high specific degradation rate.When o-Xylol concentration is 20 mg/L, the o-Xylol specific degradation rate reaches maximum value 0.19h -1.
Embodiment tri-, zoogloea resiniphilamineralization rate and the average yield coefficient analysis of HJ1 to o-Xylol.
Under optimum environment factor condition (nutrient solution pH7,35 ℃ of culture temperature), investigated bacterial strain zoogloea resiniphilamineralization rate and the average yield coefficient of HJ1 to initial o-Xylol concentration 32-258 mg/L.Result shows, described bacterial strain HJ1 can finally be converted into CO to o-Xylol 2, H 2o and cellular biomass, average mineralization rate is 54.8%, the average yield coefficient is 0.2572 mg cells/mg o-xylene.Concrete implementation step is as follows:
By bacterial suspension inoculation, in 32,64,92,128,160,192,224,256 and 288 mg/L be take the degraded substratum that o-Xylol is sole carbon source, sealing is cultivated under 35 ℃, pH=7.5 condition.Measure initial bacteria concentration and CO in culturing bottle 2concentration.Then, wait degrading when complete, add hydrochloric acid, standing half an hour, then measure final bacteria concentration and CO 2concentration.According to CO 2the changing conditions of concentration is calculated the mineralization rate of o-Xylol, according to the yield coefficient of bacteria concentration change calculations strains for degrading o-Xylol.
Result is as shown in Fig. 7 ~ 8.When o-Xylol concentration during lower than 192 mg/L, CO 2growing amount and the linear growth of o-Xylol consumption; When o-Xylol concentration during higher than 192 mg/L, CO 2growing amount tends towards stability.When o-Xylol concentration during lower than 192 mg/L, CO 2it is y=1.821x that the matching of growing amount and o-Xylol consumption is closed, and this is CO 2the ratio of growing amount and o-Xylol consumption, consume the 1mg o-Xylol and can produce 1.821mgCO 2.In theory, be entirely H when o-Xylol 2o and CO 2the time, CO 2the pass of growing amount and o-Xylol consumption is y=3.32x.When this shows that o-Xylol is degradable by bacterial strain HJ1, the mineralization rate of C is 54.8%.When o-Xylol concentration during lower than 192 mg/L, biomass and the linear growth of o-Xylol consumption; When toluene concentration, during higher than 192 mg/L, biomass sharply descends.When toluene concentration, during lower than 192 mg/L, bacterial strain concentration consumes concentration degree of fitting (R to toluene 2) being greater than 0.99, the slope of straight line is the cell yield coefficient, is 0.2572 mg cells/mg o-xylene.
Embodiment tetra-, zoogloea resiniphilahJ1 is to the common organic pollutant degradation performance analysis of industry
Using respectively in the industry such as benzene, toluene, ethylbenzene, chlorobenzene and common are organic pollutants as sole carbon source, investigate bacterial strain zoogloea resiniphilahJ1 is for the degradation capability of these materials.Result shows, the bacterial strain HJ1 above-mentioned organic pollutant of degrading to some extent, degrading benzene thing comparatively fully, to the degradation rate of chlorobenzene, hexanaphthene etc. at 8-42%.Specific embodiments is as follows:
Preparation pH is 7.3, inorganic salt nutrient solution 800 mLs of salt concn (take NaCl) as 0.66%, is sub-packed in the saline bottle of 250 mL every bottle of 50 mL, 110 ℃ of sterilizing 40 min.After nutrient solution is cooling, get wherein 8 bottles and add the bacteria suspension in logarithmic phase HJ1, make initial biomass reach 38.4 mg/L(in wet thallus), using respectively benzene, toluene, ethylbenzene, chlorobenzene etc. as sole carbon source, starting point concentration is 100 mg/L; Separately getting 8 bottles adds the above-mentioned organism of same amount but does not add the HJ1 bacteria suspension as blank.The saline bottle sealing is placed on the shaking table shaking culture of 33 ℃, respectively at 48h, analyzes residual organic concentration in liquid phase, measures corresponding biomass, calculates the degradation rate of each material.
Result as shown in Figure 9.The molecular structure of organic pollutant, accept or provide the ability of electronics to determine its chemical stability, and affecting microorganism to organic absorption and transfer ability, thereby determining organic biodegradable.Because BETX has the structure of phenyl ring, bacterial strain HJ1 has relevant oxygenase, can be by BETX catalyzed degradation effectively.Bacterial strain HJ1 can not degrade and have the chlorobenzene of benzene ring structure, may be because the Cl on phenyl ring makes the electrophilic substitution reaction passivation.And methyl halide, the tetradecane, sherwood oil (main component is pentane and hexane), the structure of the compounds such as hexanaphthene and tetrahydrobenzene and the difference of BETX are larger, therefore bacterial strain HJ1 can not carry out degradable to it.Above test-results explanation, bacterial strain zoogloea resiniphilathe HJ1 multiple common organic pollutant of degrading, have certain directive significance for this bacterial strain for the actual industrial Pollution abatement.
Although the present invention with embodiment openly as above; but it is not in order to limit protection scope of the present invention; any technician who is familiar with this technology, not breaking away from change and the retouching of doing in the spirit and scope of the present invention, all should belong to protection scope of the present invention.
<110 > Zhejiang Polytechnical University
<120 > strain has moving glue bacterium and the application thereof of o-Xylol degradation capability
<160> 1
<210>1
<211>1440
<212> DNA
<213>moving glue bacterium ( zoogloea resiniphila)
<400>1
ttggggcggc agctttccat gcaagtcgaa cggcagcacg ggcttcggcc tggtggcgag <60>
tggcgaacgg gtgagtaatg catcggaacg tacccagtcg tgggggataa cgtagcgaaa <120>
gttacgctaa taccgcatac gtcctgaggg agaaagcggg ggaccgtaag gcctcgcgcg <180>
attggagcgg ccgatgtcgg attagctagt tggtggggta aaggcctacc aaggcgacga <240>
tccgtagcgg gtctgagagg atgatccgcc acactgggac tgagacacgg cccagactcc <300>
tacgggaggc agcagtgggg aattttggac aatgggcgaa agcctgatcc agccatgccg <360>
cgtgagtgaa gaaggccttc gggttgtaaa gctctttcag acggaaagaa atcttctggg <420>
ctaataccct gggaggatga cggtaccgta agaagaagca ccggctaact acgtgccagc <480>
agccgcggta atacgtaggg tgcgagcgtt aatcggaatt actgggcgta aagcgtgcgc <540>
aggcggtgat gtaagacaga tgtgaaatcc ccgggctcaa cctgggaact gcgtttgtga <600>
ctgcatcact cgagtacggc agagggaggt ggaattccgc gtgtagcagt gaaatgcgta <660>
gagatgcgga ggaacaccga tggcgaaggc agcctcctgg gccagtactg acgctcatgc <720>
acgaaagcgt ggggagcaaa caggattaga taccctggta gtccacgccc taaacgatgt <780>
caactagttg ttcggtgagg agactcattg agtaacgcag ctaacgcgtg aagttgaccg <840>
cctggggagt acggccgcaa ggttaaaact caaaggaatt gacggggacc cgcacaagcg <900>
gtggatgatg tggattaatt cgatgcaacg cgaaaaacct tacctaccct tgacatgcca <960>
ggaacttgcc agagatggct tggtgctcga aagagagcct ggacacaggt gctgcatggc <1020>
tgtcgtcagc tcgtgtcgtg agatgttggg ttaagtcccg caacgagcgc aacccttgtc <1080>
attagttgcc atcattaagt tgggcactct aatgagactg ccggtgacaa accggaggaa <1140>
ggtggggatg acgtcaagtc ctcatggccc ttatgggtag ggcttcacac gtcatacaat <1200>
ggtcggtaca gagggttgcc aagccgcgag gtggagccaa tcccagaaag ccgatcgtag <1260>
tccggattgg agtctgcaac tcgactccat gaagtcggaa tcgctagtaa tcgcagatca <1320>
gcatgctgcg gtgaatacgt tcccgggtct tgtacacacc gcccgtcaca ccatgggagt <1380>
ggggtttacc agaagtaggt agcttaaccg caaggagggc gctaccacgt agctcgtccc <1440>

Claims (7)

  1. One strain have the o-Xylol degradation capability moving glue bacterium ( zoogloea resiniphila) HJ1, its deposit number is CCTCC NO.M2012235.
  2. 2. the application of moving glue bacterium HJ1 in degraded o-Xylol organic pollutant with o-Xylol degradation capability claimed in claim 1.
  3. 3. the application of moving glue bacterium HJ1 in degraded benzene, toluene, ethylbenzene, dimethylbenzene, chlorobenzene organic pollutant with o-Xylol degradation capability claimed in claim 1.
  4. 4. application according to claim 2 is characterized in that: the described moving glue bacterium HJ1 with o-Xylol degradation capability is put in the sewage containing o-Xylol through cultivating the bacteria suspension obtained, and is 25-35 ℃ in temperature, under the condition of pH value for 7-9, cultivates.
  5. 5. application according to claim 4 is characterized in that: described moving glue bacterium HJ1 optimum culturing temperature in waste water with o-Xylol degradation capability is 35 ℃, pH7, and the o-Xylol degradation rate is 5.33 mg/ (Lh).
  6. 6. application according to claim 5, it is characterized in that: the described moving glue bacterium HJ1 degraded o-Xylol concentration range with o-Xylol degradation capability is 0-256 mg/L, and the high specific degradation rate is 0.19 mg o-Xylol/(mg cellsh).
  7. 7. application according to claim 5 is characterized in that: the described moving glue bacterium HJ1 with o-Xylol degradation capability finally is converted into CO to o-Xylol 2, H 2o and cellular biomass, its average yield coefficient is 0.2572 mg cells/mg o-Xylol, average mineralization rate is 54.8%.
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CN104673707B (en) * 2014-12-24 2018-04-27 浙江工业大学 " fungi-bacterium " compound micro-ecological preparation, its preparation method and its application in the processing of VOCs mix waste gas
US10350546B2 (en) * 2014-12-24 2019-07-16 Jianmeng Chen Fungi-bacteria composite microecologics and methods for preparing and using the same
CN111974358A (en) * 2020-08-20 2020-11-24 常州良福朗清生物科技有限公司 Preparation method and application of microbial adsorbent

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