CN103435680A - Method for extracting protein peptide from beans - Google Patents
Method for extracting protein peptide from beans Download PDFInfo
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- CN103435680A CN103435680A CN2013103264045A CN201310326404A CN103435680A CN 103435680 A CN103435680 A CN 103435680A CN 2013103264045 A CN2013103264045 A CN 2013103264045A CN 201310326404 A CN201310326404 A CN 201310326404A CN 103435680 A CN103435680 A CN 103435680A
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Abstract
The invention relates to a method for extracting a protein peptide from beans. The method comprises the steps that the beans are made into pulp, and an extractive of the protein peptide in the beans is obtained by micro-filtration membrane filtration, ultra-filtration membrane filtration and nano-filtration membrane filtration. The method solves the problems that the protein peptide is low in extraction efficiency and high in extraction cost and the protein peptide in the extractive is low in mass content in the prior art; more than 95% of low-molecular protein peptide is extracted through concentration; the extraction efficiency of the protein peptide is improved; the method is particularly suitable for extracting the protein peptide from the beans; and the mass content of the protein peptide in the extractive is about 80%.
Description
Technical field
The present invention relates to a kind of separating substances extractive technique method, particularly a kind of method of extracting protein peptide from beans.
Background technology
Protein peptide not only has multiple body metabolism and physiological regulation function, can also provide nutrition for body, there is antibiotic, antiviral, hypotensive, reducing blood-fat and increase the physiological functions such as immunizing power, edible safety is high, is the research topic that current food science and technology is the most popular and the functional factor that has development prospect.
Utilize the protein in food, extract various active polypeptide with difference in functionality, as the nutritional additive of food, the transformation traditional food, develop new heath food, becomes many nutritionists' research topic; In Japan, the U.S. and West Europe, the heath food that contains polypeptide emerges in an endless stream, for example polypeptide beverage, polypeptide children lunch, polypeptide old man set meal, polypeptide health care biscuit etc.; In China, the nutritionist is also carrying forward vigorously the application of polypeptide in food, particularly derive from the polypeptide in food proteins, many monographs and paper publishing were arranged in recent years, also there are some polypeptide heath food to come out, can say, the application of polypeptide in heath food has incomparable huge commercial promise.
In beans, protein and content of peptides are higher, and the traditional extraction process of beans, particularly Semen Pisi sativi protein has alkali extraction and acid precipitation method, salt precipitation method, organic solvent precipitation method etc.; The molecular weight of Semen Pisi sativi protein is lower, good water solubility, the extraction difficulty is large, the Semen Pisi sativi protein majority has only been accomplished feed grade albumen at present, in these methods, some method yields are too low, and contain the starch of some amount etc. in the protein of traditional method for extracting and polypeptide, the content of extract obtained middle protein generally can only reach 60% left and right; Some method costs are too high as organic solvent precipitation.
Membrane sepn is a new isolation technique.Membrane separation technique is owing to having separation, concentrated, purifying and refining function concurrently, efficient, energy-saving and environmental protection are arranged again, molecular level filters and filtration procedure is simple, be easy to the feature such as control, be widely used at present food, medicine, biology, environmental protection, chemical industry, metallurgy, the energy, oil, water treatment, electronics, the field such as bionical, become one of most important means in current separation science.
The Chinese invention patent that Granted publication number is " CN 1687123 " discloses a kind of membrane process for extracting soybean protein peptide and byproduct deep processing method thereof, the step of extracting protein peptide in the method comprises: 1) the low temperature soybean meal is through sig water lixiviate 10-20 hour, the alkali lye feed liquid proportion 1:10-20 that is weight percentage, the pH value is 7.5-9.0, after utilizing whizzer to isolate bean dregs after immersion, utilize the polysulfone membrane ultrafiltration, separating whey solution and protein solution; 2) protein solution is carried out to temperature 105-115 ℃, the instantaneous sterilization of time 3-5s, after sterilization, protein solution is homogenized; 3) protein solution after homogeneous is concentrated, spraying drying, obtain the finished product soybean protein isolate, but the method due to being separated, bean dregs also has some protein in bean dregs, caused the loss of protein, simultaneously, the method has only adopted the polysulfone membrane ultrafiltration, also contains certain protein in isolated whey solution, thereby, utilize the method to proteins extraction is thorough, efficiency is low.
Summary of the invention
The object of the present invention is to provide a kind of method of extracting protein peptide from beans, to solve, in prior art, the proteins and peptides extraction efficiency is low, extraction cost is high, the low problem of proteins and peptides mass content in extract.
Technical scheme of the present invention is achieved in that a kind of method of extracting protein peptide from beans, it is characterized in that, it comprises the following steps:
1) slurrying: beans is made to soup compound, regulate the pH value of described soup compound, with food grade sodium hydroxide, the pH value of described soup compound is adjusted into to 7.5-11.0, obtain the micro-filtration feed liquid;
2) micro-filtrate membrane filtration: above-mentioned micro-filtration feed liquid, by micro-filtrate membrane filtration, is obtained to the first filtrate and filter residue; Above-mentioned filter residue is put into to the food grade sodium hydroxide solution that the pH value is 7.5-11.0 again and soak 1-5 hour, then use micro-filtrate membrane filtration, obtain the second filtrate, then above-mentioned the first filtrate and described the second filtrate are combined, and the pH value of above-mentioned merging filtrate is adjusted into to 7.5-11.0, be the ultrafiltration feed liquid;
3) ultrafiltration membrance filter: by the described ultrafiltration feed liquid of second step through the ultra-filtration membrane thickening that circulated, stop thickening when the massfraction of protein is 15-20% in ultrafiltration and concentration liquid, after ultra-filtration membrane circulation thickening, gained solution is called the first concentrated solution and first through liquid;
4) protein peptide is extracted for the first time: with food grade hydrochloric acid, the pH value of the first above-mentioned concentrated solution is adjusted into to 6.5-7.5, obtains the protein peptide that molecular weight is 0.6-10 ten thousand MW after drying;
5) nanofiltration membrane is filtered: the first above-mentioned pH value that sees through liquid is adjusted into to 7.5-11.0, for the nanofiltration feed liquid, the nanofiltration feed liquid is through the nanofiltration membrane thickening that circulated, stop thickening when the massfraction of proteins and peptides is 10-20% in the nanofiltration concentrated solution, after nanofiltration membrane circulation thickening, gained solution is that the second concentrated solution and second sees through liquid;
6) protein peptide is extracted for the second time: with food grade hydrochloric acid, the pH value of the second above-mentioned concentrated solution is adjusted into to 6.5-7.5, obtains the protein peptide that molecular weight is less than 6000MW after drying.
The pH value of nanofiltration feed liquid described in step 5 of the present invention adopts on-line monitoring.
The described dry spraying drying that adopts of step 4 of the present invention and step 6.
The described microfiltration membrane pore size of step 2 of the present invention is 1-50 μ m, and described micro-filtrate membrane filtration equipment adopts plate-and-frame filter press or cloth envelop collector.
The pore size of the described ultra-filtration membrane of step 3 of the present invention is 1-15nm, and described ultra-filtration membrane is polyvinylidene fluoride (PVDF) ultrafiltration membrane or polypropylene ultra-filtration membrane.
The described nanofiltration membrane of step 5 of the present invention is the nanofiltration membrane that molecular weight cut-off is 150-1000MW.
In the method for extracting protein peptide from beans of the present invention, the pH value of the nanofiltration feed liquid that the ultrafiltration feed liquid of the micro-filtration feed liquid of described micro-filtrate membrane filtration, described ultrafiltration membrance filter and described nanofiltration membrane are filtered is 7.5-11.0; Beans during particularly the Semen Pisi sativi protein peptide extracts process of manufacture, proteinaceous solution is neutral or acid, during due to isoelectric point of protein, pH is 4.3-4.7, protein solubleness in neutrality or acidic solution is low, have a large amount of protein to exist with coagulation, and these floccular albumen are harmful to very much for membrane sepn, their viscosity is large, easily be attached to the surface of film, form film and stop up and have a strong impact on extraction efficiency; With food grade sodium hydroxide, the pH value of described micro-filtration feed liquid, described ultrafiltration feed liquid, described nanofiltration feed liquid all is adjusted into to 7.5-11.0 in the inventive method, in this value scope, can makes albumen dissolve fully, greatly increase the extraction efficiency of protein peptide in beans; In addition, protein dissolves fully, although solution still has certain viscosity, but can not stop up described ultra-filtration membrane and described nanofiltration membrane, use the inventive method to extract ultra-filtration membrane described in the production of proteins and peptides from beans and described nanofiltration membrane is easy to cleaning, can recover the membrane flux of described ultra-filtration membrane and described nanofiltration membrane by simple flushing, be beneficial to the use that increases described ultra-filtration membrane and described nanofiltration membrane.
The inventive method is put into by described filter residue the food grade sodium hydroxide solution that the pH value is 7.5-11.0 and is soaked 1-5 hour, then uses micro-filtrate membrane filtration, obtains the second filtrate; Because protein content in described filter residue still accounts for the 13-15% of described filter residue total amount, so the utilization again of filter residue is most important for increasing the proteins and peptides extraction efficiency.
The high molecular weight protein peptide that ultra-filtration membrane can only molecular weight cut-off is 6000-10 ten thousand MW, and can not hold back the micromolecular protein peptide that molecular weight is less than 6000MW, the micromolecular protein peptide that molecular weight is less than 6000MW becomes the liquid that sees through of device for ultrafiltration membrane together with water, by nanofiltration membrane, the liquid that sees through of ultra-filtration membrane is carried out to thickening, by 95% above low molecular protein peptide concentration extraction, improved the protein peptide extraction efficiency, and in extract, the mass content of protein peptide is 80% left and right, the protein peptide that the inventive method is extracted gained is of high nutritive value; In addition, the seeing through in liquid of nanofiltration membrane sees through the liquid nitrogen content and can reach 0.02%, and the composition of these nitrogen is mainly by amino acids formed, sees through water as clear as crystal, can reuse or enter sewage work.
The present invention adopts plate-and-frame filter press or cloth envelop collector to carry out described micro-filtrate membrane filtration, and plate-and-frame filter press and cloth envelop collector are filter plant commonly used, and filtering accuracy is high, treatment capacity is large, with low cost, working service convenient.
The pH value of nanofiltration feed liquid of the present invention adopts on-line monitoring, can guarantee that like this nanofiltration membrane is not blocked, makes nanofiltration membrane be easy to clean, and increases the work-ing life of described nanofiltration membrane.
The dry spraying drying that adopts of the present invention, spraying drying can directly make solution, emulsion be dried to powder or particulate state goods, technique is simple, and this drying means does not have destructiveness to extracted proteins and peptides, the proteins and peptides nutritive value that increase the inventive method is extracted.
Microfiltration membrane pore size of the present invention is 1-50 μ m, adopt the microfiltration membrane of this pore size, can the effectively catching Mierocrystalline cellulose etc. impurity, make proteins and peptides see through this film, improve the extraction efficiency of extracting proteins and peptides from beans of the present invention, and in extract, the content of proteins and peptides is high.
At first the method for extracting protein peptide from beans of the present invention is used the high molecular weight protein peptide of ultra-filtration membrane Extraction parts molecular weight for 6000-10 ten thousand MW, the liquid that sees through of ultra-filtration membrane is less than 95% above molecular weight through nanofiltration membrane treatment the low molecular protein peptide concentration extraction of 6000MW again, improved the protein peptide extraction efficiency, and in extract, the mass content of protein peptide is 80% left and right, simultaneously, use destructive reagent and other the destructive means that can destroy the protein peptide activity in the method for extracting protein peptide from beans of the present invention, the protein peptide of extracting gained is of high nutritive value.
Embodiment
The method of extracting protein peptide from beans of the present invention, it comprises the following steps:
1) slurrying: beans is made to soup compound, this beans is pea, and soup compound is inserted to the magma tank, then regulates the pH value of described soup compound, in the magma tank, add while stirring food grade sodium hydroxide that the pH value of described soup compound is adjusted into to 7.5-11.0, obtain the micro-filtration feed liquid;
2) micro-filtrate membrane filtration: the micro-filtration feed liquid that the pump of take obtains step 1) as motivating force is transported in microfiltration membrane is filtered, and the microfiltration membrane pore size is 1-50 μ m, and micro-filtrate membrane filtration equipment adopts plate-and-frame filter press or cloth envelop collector, obtains the first filtrate and filter residue; The first filtrate enters the ultrafiltration circulation tank, filter residue in micro-filtrate membrane filtration equipment is transferred in the washery slag tank, soak 1-5 hour in the food grade sodium hydroxide solution that is 7.5-11.0 in the pH value, and then filter with micro-filtrate membrane filtration equipment, obtain the second filtrate, the second filtrate enters in the ultrafiltration circulation tank, and the filter residue again produced is for the production of Mierocrystalline cellulose or be used as him, it is 7.5-11.0 that feed liquid in the ultrafiltration circulation tank is adjusted to the pH value with food grade sodium hydroxide, is the ultrafiltration feed liquid;
3) ultrafiltration membrance filter: the ultrafiltration feed liquid in the ultrafiltration circulation tank be take to pump and be transported in device for ultrafiltration membrane the thickening that circulated as motivating force, the pore size of ultra-filtration membrane is 1-15nm, ultra-filtration membrane is polyvinylidene fluoride (PVDF) ultrafiltration membrane or polypropylene ultra-filtration membrane, stop thickening when the massfraction of protein is 15-20% in ultrafiltration and concentration liquid, after ultra-filtration membrane circulation thickening, gained solution is called the first concentrated solution and first through liquid;
4) protein peptide is extracted for the first time: with pump, the first concentrated solution is transported in setting tank, with food grade hydrochloric acid, the pH value of the first above-mentioned concentrated solution is adjusted into to 6.5-7.5 again, then carry out spraying drying, the protein peptide that the molecular weight that to obtain the protein quality mark be 78% is 0.6-10 ten thousand MW;
5) nanofiltration membrane is filtered: by step 3) first see through liquid by pump delivery in the nanofiltration circulation tank, see through liquid by first and be adjusted into 7.5-11.0 by food grade sodium hydroxide adjusting pH value in the nanofiltration circulation tank, for the nanofiltration feed liquid, by high-pressure pump, be that motivating force is transported to the nanofiltration feed liquid in nano-filtration membrane equipment and filters, carry out thickening, nanofiltration membrane is the nanofiltration membrane that molecular weight cut-off is 150-1000MW, stop thickening when the massfraction of proteins and peptides is 10-20% in the nanofiltration concentrated solution, after nanofiltration membrane circulation thickening, gained solution is that the second concentrated solution and second sees through liquid, the second protein quality mark seen through in liquid is 0.02%, drain and enter sewage work or recycling,
6) peptide and protein extracts: the second concentrated solution by pump delivery in another setting tank, with food grade hydrochloric acid, the pH value of the second above-mentioned concentrated solution is adjusted into to 6.5-7.5, then carry out spraying drying, obtain the polypeptide of protein quality mark 82% and the albumen that molecular weight is less than 6000MW.
Claims (8)
1. a method of extracting protein peptide from beans, is characterized in that, it comprises the following steps:
1) slurrying: beans is made to soup compound, regulate the pH value of described soup compound, with food grade sodium hydroxide, the pH value of described soup compound is adjusted into to 7.5-11.0, obtain the micro-filtration feed liquid;
2) micro-filtrate membrane filtration: above-mentioned micro-filtration feed liquid, by micro-filtrate membrane filtration, is obtained to the first filtrate and filter residue; Above-mentioned filter residue is put into to the food grade sodium hydroxide solution that the pH value is 7.5-11.0 again and soak 1-5 hour, then use micro-filtrate membrane filtration, obtain the second filtrate, then above-mentioned the first filtrate and described the second filtrate are combined, and the pH value of above-mentioned merging filtrate is adjusted into to 7.5-11.0, be the ultrafiltration feed liquid;
3) ultrafiltration membrance filter: by the described ultrafiltration feed liquid of second step through the ultra-filtration membrane thickening that circulated, stop thickening when the massfraction of protein is 15-20% in ultrafiltration and concentration liquid, after ultra-filtration membrane circulation thickening, gained solution is called the first concentrated solution and first through liquid;
4) protein peptide is extracted for the first time: with food grade hydrochloric acid, the pH value of the first above-mentioned concentrated solution is adjusted into to 6.5-7.5, obtains the protein peptide that molecular weight is 0.6-10 ten thousand MW after drying;
5) nanofiltration membrane is filtered: the first above-mentioned pH value that sees through liquid is adjusted into to 7.5-11.0, for the nanofiltration feed liquid, the nanofiltration feed liquid is through the nanofiltration membrane thickening that circulated, stop thickening when the massfraction of proteins and peptides is 10-20% in the nanofiltration concentrated solution, after nanofiltration membrane circulation thickening, gained solution is that the second concentrated solution and second sees through liquid;
6) protein peptide is extracted for the second time: with food grade hydrochloric acid, the pH value of the second above-mentioned concentrated solution is adjusted into to 6.5-7.5, obtains the protein peptide that molecular weight is less than 6000MW after drying.
2. the method for extracting protein peptide from beans according to claim 1, is characterized in that the pH value of the feed liquid of nanofiltration described in step 5 adopts on-line monitoring.
3. the method for extracting protein peptide from beans according to claim 1, is characterized in that the described dry spraying drying that adopts of step 4 and step 6.
4. the method for extracting protein peptide from beans according to claim 1, is characterized in that the described microfiltration membrane pore size of step 2 is 1-50 μ m.
5. according to the described method of extracting protein peptide from beans of claim 1 or 4, it is characterized in that described micro-filtrate membrane filtration equipment adopts plate-and-frame filter press or cloth envelop collector.
6. the method for extracting protein peptide from beans according to claim 1, the pore size that it is characterized in that the described ultra-filtration membrane of step 3 is 1-15nm.
7. according to the described method of extracting protein peptide from beans of claim 1 or 6, it is characterized in that described ultra-filtration membrane is polyvinylidene fluoride (PVDF) ultrafiltration membrane or polypropylene ultra-filtration membrane.
8. the method for extracting protein peptide from beans according to claim 1, is characterized in that the described nanofiltration membrane of step 5 is the nanofiltration membrane that molecular weight cut-off is 150-1000MW.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN105755080A (en) * | 2016-03-07 | 2016-07-13 | 湖北工业大学 | Production method of Sacha inchi polypeptide |
| CN106800585A (en) * | 2017-01-17 | 2017-06-06 | 成都美富特膜科技有限公司 | The method and apparatus that alpha amylase suppresses albumen is extracted from beans powder slurries |
| CN109095716A (en) * | 2018-08-17 | 2018-12-28 | 河南民兴生物科技股份有限公司 | A kind of production wastewater treatment system containing sericin |
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| WO2012155256A1 (en) * | 2011-05-19 | 2012-11-22 | Burcon Nutrascience (Mb) Corp. | Production of soluble soy protein product ("s704") |
| CN103012580A (en) * | 2012-12-07 | 2013-04-03 | 成都连接流体分离科技有限公司 | Method for recycling of soybean whey wastewater by membrane separation technology |
| CN103113459A (en) * | 2013-03-01 | 2013-05-22 | 秦向东 | Method for continuously producing whey soy proteins through soy whey water |
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| CN1740189A (en) * | 2005-09-20 | 2006-03-01 | 武汉湖工大膜技术开发有限公司 | Method of extracting and separating soybean protein |
| CN101974589A (en) * | 2010-10-29 | 2011-02-16 | 江南大学 | Method for preparing immunocompetent soybean peptide by enzymolysis and membrane separation |
| WO2012155256A1 (en) * | 2011-05-19 | 2012-11-22 | Burcon Nutrascience (Mb) Corp. | Production of soluble soy protein product ("s704") |
| CN103012580A (en) * | 2012-12-07 | 2013-04-03 | 成都连接流体分离科技有限公司 | Method for recycling of soybean whey wastewater by membrane separation technology |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105755080A (en) * | 2016-03-07 | 2016-07-13 | 湖北工业大学 | Production method of Sacha inchi polypeptide |
| CN105755080B (en) * | 2016-03-07 | 2020-02-18 | 湖北工业大学 | Preparation method of Plukenetia volubilis Linn polypeptide |
| CN106800585A (en) * | 2017-01-17 | 2017-06-06 | 成都美富特膜科技有限公司 | The method and apparatus that alpha amylase suppresses albumen is extracted from beans powder slurries |
| CN109095716A (en) * | 2018-08-17 | 2018-12-28 | 河南民兴生物科技股份有限公司 | A kind of production wastewater treatment system containing sericin |
| CN109095716B (en) * | 2018-08-17 | 2023-11-24 | 河南民兴生物科技股份有限公司 | Production wastewater treatment system containing sericin |
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Application publication date: 20131211 |