CN103434764A - Storage and transportation method of dendrobium officinale tissue culture seedlings - Google Patents

Storage and transportation method of dendrobium officinale tissue culture seedlings Download PDF

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Publication number
CN103434764A
CN103434764A CN201310393744XA CN201310393744A CN103434764A CN 103434764 A CN103434764 A CN 103434764A CN 201310393744X A CN201310393744X A CN 201310393744XA CN 201310393744 A CN201310393744 A CN 201310393744A CN 103434764 A CN103434764 A CN 103434764A
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China
Prior art keywords
chest
root
tissue culture
seedling
box
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CN201310393744XA
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Chinese (zh)
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CN103434764B (en
Inventor
何晔
陈彦海
周广坤
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JINING SENLI BIOLOGICAL TECHNOLOGY Co Ltd
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JINING SENLI BIOLOGICAL TECHNOLOGY Co Ltd
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Priority to CN201310393744.XA priority Critical patent/CN103434764B/en
Publication of CN103434764A publication Critical patent/CN103434764A/en
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Publication of CN103434764B publication Critical patent/CN103434764B/en
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  • Cultivation Receptacles Or Flower-Pots, Or Pots For Seedlings (AREA)
  • Cultivation Of Plants (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)

Abstract

The invention provides a storage and transportation method of dendrobium officinale tissue culture seedlings. The method comprises the following steps: firstly, lightly taking out the domesticated tissue culture seedlings, cleaning the root parts in clean water without injuring the root parts attentively, and then fishing out and placing in the shade to remove surface moisture; punching 2-3 holes of which the diameters are 1cm in the middle of each side of the prepared foam box, wherein the box is a hexahedron and the distance between the holes is greater than 2cm; paving a layer of cobbles on the bottom of the box; orderly putting the dried tissue culture seedlings inside the box in a manner that root parts are opposite, and paving a layer of sphagna of which the humidity is 90-95% at the upper part of the root part of each layer which is 5cm thick; sealing the box by an adhesive tape after properly placing, and putting in the shade, so as to transport. The storage and transportation method provided by the invention is small in cost and high in survival rate in long-distance transportation of the dendrobium officinale tissue culture seedlings.

Description

A kind of conveying method of candidum tissue culturing seedling
Technical field
The present invention relates to the method for preserving of a kind of group of training seedling, belong to the logistics transportation technical field.
Background technology
In the training of the routine group of dendrobium candidum, group training seedling is to take root in bottle, and then hardening goes out garden again, like this can assurance group training seedling and moisture and the nutrient transport system of culture, can the raising group train the transplanting survival rate of seedling.But present group training seedling generally is with culture along with glass culture bottle or culture bag transportation, and this accumulating mode, occupy space large, and culture bottle is frangible, to transportation, brings inconvenience; For producer, culture bottle or culture bag repeated usage again, do not have too large practical value to the buyer yet, generally as garbage disposal, falls, and so just causes the significant wastage of resource; Simultaneously, culture bottle or culture bag are subject to the impact of temperature in transportation, affect the survivor's rate of group training seedling; And, after group training seedling has been bought at the plantation family, the processing before need to planting group training seedling, due to the restriction of the volume of culture bottle or culture bag, its work capacity is huge, and careless slightlyly will cause damage to the root of seedling, be lowered into motility rate, increase the cost at plantation family.
Summary of the invention
In order to solve the problems of the technologies described above, the invention provides a kind of conveying method of candidum tissue culturing seedling.
That conveying method provided by the invention spends in the long-distance transport of candidum tissue culturing seedling is little, survivor's rate is high.
Technical scheme of the present invention is as follows:
A kind of conveying method of candidum tissue culturing seedling, comprise the steps:
A, first the training of the group after hardening seedling is taken out gently, clean root in clear water, note not hindering its root, then pull out in the cool the seedling surface moisture that dries in the shade.
B, ready foam chest, described chest is hexahedron, at the middle part of every of chest, beats the aperture that 2-3 diameter is 1 centimetre, the distance of aperture is greater than 2 centimetres, and at chest bottom layer overlay stone.
C, relative the group of drying in the shade training shoot root section, the neat chest the inside that is placed in, the sphagna that to make 5 centimetres of thickness be one deck is 90-95% the root top layer overlay humidity of every layer.Sphagna can not be put into leaf and the stem of seedling, in case there is corruption to occur.
D, set and seal chest with adhesive tape and place in the cool afterwards, in order to transportation.
The present invention has following beneficial effect:
1, save space.For example, bottled 3000 seedlings, 6 baskets of bottles, and occupation of land will at least 2 cubic meters: if use bubble chamber, size 40cm*30cm*30cm, can install for one the case, only 4 yuan, each chest, save space, saved tissue culture bottle simultaneously, thereby reduced productive costs and traffic cost.
2, fresh keeping time is long.Foam chest after treatment provided by the invention has insulation, refrigeration, fresh-keeping effect, and cracky not, temperature in chest maintains 18 ℃-22 ℃, humidity in chest maintains between 95%--99%, be subject to the impact of extreme weather less, seedling on the way transports does not still affect original profile and life in 10 days afterwards.
3, saving group training seedling migration process operation.Group training seedling is processed, can directly plant, and seedling is not dealt with the work again, and saves a large amount of human and material resources and financial resources.
4, the survivor's rate of group training seedling is high.The inventive method, the survivor's rate of group training seedling can reach more than 90%.
The specific embodiment
Below in conjunction with embodiment, the present invention is further illustrated.
Embodiment 1
The conveying method of candidum tissue culturing seedling provided by the invention, comprise the steps:
A, first the training of the group after hardening seedling is taken out gently, clean root in clear water, note not hindering its root, then pull out in the cool the seedling surface moisture that dries in the shade;
B, ready foam chest, described chest is hexahedron, at the middle part of every of chest, beats the aperture that 2-3 diameter is 1 centimetre, the distance of aperture is greater than 2 centimetres, and at chest bottom layer overlay stone; Laying stone is in order to ventilate, and prevents bottom rot;
C, relative the group of drying in the shade training shoot root section, the neat chest the inside that is placed in, the sphagna that to make 5 centimetres of thickness be one deck is 90-95% the root top layer overlay humidity of every layer; Sphagna can not be put into leaf and the stem of seedling, in case there is corruption to occur;
D, set and seal chest with adhesive tape and place in the cool afterwards, in order to transportation.

Claims (1)

1. the conveying method of a candidum tissue culturing seedling, is characterized in that, comprises the steps:
A, first the training of the group after hardening seedling is taken out gently, clean root in clear water, note not hindering its root, then pull out in the cool the seedling surface moisture that dries in the shade;
B, ready foam chest, described chest is hexahedron, at the middle part of every of chest, beats the aperture that 2-3 diameter is 1 centimetre, the distance of aperture is greater than 2 centimetres, and at chest bottom layer overlay stone;
c, relative the group of drying in the shade training shoot root section, the neat chest the inside that is placed in, the sphagna that to make 5 centimetres of thickness be one deck is 90-95% the root top layer overlay humidity of every layer;
D, set and seal chest with adhesive tape and place in the cool afterwards, in order to transportation.
CN201310393744.XA 2013-09-03 2013-09-03 Storage and transportation method of dendrobium officinale tissue culture seedlings Active CN103434764B (en)

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CN201310393744.XA CN103434764B (en) 2013-09-03 2013-09-03 Storage and transportation method of dendrobium officinale tissue culture seedlings

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CN103434764A true CN103434764A (en) 2013-12-11
CN103434764B CN103434764B (en) 2015-05-13

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Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104843352A (en) * 2015-05-12 2015-08-19 柳州市耕青科技有限公司 Preservation method for dendrobium officinale in transportation
CN105284574A (en) * 2015-12-02 2016-02-03 东莞市粮作花卉研究所 Method for conducting bottle-removed storage and transportation on bottled dendrobium seedlings
CN105857833A (en) * 2016-05-26 2016-08-17 霍山宝信园石斛开发有限公司 Box-like freshness retaining device in fresh stem of noble dendrobium strip transportation process
CN106005745A (en) * 2016-06-28 2016-10-12 桐城市雨润生物科技有限公司 Box-shaped freshness preservation device used during transportation process of fresh dendrobium stems

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
NL9000582A (en) * 1990-03-14 1991-10-01 Pothos Holding B V Seedling transport box - supports several standard-size plant trays spaced apart
CN1460401A (en) * 2003-06-12 2003-12-10 中国科学院武汉植物研究所 Introduction method of king-lotus offspring
CN2925076Y (en) * 2006-04-30 2007-07-25 阎学军 Container for culturing, packing and transporting bean sprouts
CN202912162U (en) * 2012-10-16 2013-05-01 广西橡胶研究所 Box for transporting tissue culture seedlings

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
NL9000582A (en) * 1990-03-14 1991-10-01 Pothos Holding B V Seedling transport box - supports several standard-size plant trays spaced apart
CN1460401A (en) * 2003-06-12 2003-12-10 中国科学院武汉植物研究所 Introduction method of king-lotus offspring
CN2925076Y (en) * 2006-04-30 2007-07-25 阎学军 Container for culturing, packing and transporting bean sprouts
CN202912162U (en) * 2012-10-16 2013-05-01 广西橡胶研究所 Box for transporting tissue culture seedlings

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
蒙平,张向军,何新民: "《铁皮石斛组培苗移栽新技术》", 《中国热带农业》 *

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104843352A (en) * 2015-05-12 2015-08-19 柳州市耕青科技有限公司 Preservation method for dendrobium officinale in transportation
CN105284574A (en) * 2015-12-02 2016-02-03 东莞市粮作花卉研究所 Method for conducting bottle-removed storage and transportation on bottled dendrobium seedlings
CN105857833A (en) * 2016-05-26 2016-08-17 霍山宝信园石斛开发有限公司 Box-like freshness retaining device in fresh stem of noble dendrobium strip transportation process
CN106005745A (en) * 2016-06-28 2016-10-12 桐城市雨润生物科技有限公司 Box-shaped freshness preservation device used during transportation process of fresh dendrobium stems

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