CN103416394A - Method for drying and storage of blood vessels - Google Patents
Method for drying and storage of blood vessels Download PDFInfo
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- CN103416394A CN103416394A CN2012104315656A CN201210431565A CN103416394A CN 103416394 A CN103416394 A CN 103416394A CN 2012104315656 A CN2012104315656 A CN 2012104315656A CN 201210431565 A CN201210431565 A CN 201210431565A CN 103416394 A CN103416394 A CN 103416394A
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Abstract
The invention provides a method for drying and storage of blood vessels, which is used for removing moisture in blood vessels and then storing the blood vessels. The method comprises the following steps: firstly, placing the blood vessels in an environment of which the temperature ranges from 65 DEG C below zero to 75 DEG C below zero and the air pressure value is the atmospheric pressure value, and enabling moisture to be frozen into crystal ice; secondly, adjusting the environment temperature to be 10 DEG C below zero to 25 DEG C below zero and the air pressure value to be 10 Pa to 50 Pa, and removing 90%-98% of moisture through sublimation of crystal ice; thirdly, adjusting the environment temperature to be 10 DEG C to 20 DEG C and the air pressure value to be 10 Pa to 50 Pa and removing the residual moisture; fourthly, storing the blood vessels in a hermetically sealed vacuum environment at the temperature of 4 DEG C-25 DEG C.
Description
Technical field
The present invention relates to biology and low temperature field, can be applicable in the preservation and clinical transplantation of blood vessel.
Background technology
The material that at present for surgery, carries out vasotransplantation can be divided into synthetic material and biological tissue material.Because biomaterial itself has baroque characteristics, modern engineering level can not be copied the 26S Proteasome Structure and Function of vascular tissue preferably, the blood vessel of synthetic material has and breaks long term and possibility that the embolism incidence is high, and its result for the treatment of is very limited; The intravital blood vessel organization material usually adopts and puts into the method that liquid nitrogen low temperature is preserved, but equipment needed thereby is huge and expensive, and the blood vessel that low temperature the is preserved incidence of disease at a specified future date is high, and the danger produced as false aneurysm and so on problem is arranged.
Goal of the invention:
The object of the present invention is to provide a kind of method of blood vessel being carried out to kept dry, the method, by adjusting temperature and the atmospheric pressure value of blood vessel environment of living in, is removed the moisture content in blood vessel, and then by above-mentioned blood vessel vacuum seal preservation at normal temperatures.Preserved to greatest extent the various structures of above-mentioned blood vessel, made it rehydration time short, performance is close to fresh blood vessel, so that clinical use; After vacuum seal, under room temperature, can preserve easily for a long time.
Summary of the invention:
The invention provides a kind of method of blood vessel being carried out to kept dry, then preserve for the moisture content of removing blood vessel.
At first, it is under-65 ℃～-75 ℃, the atmospheric pressure value environment that is atmospheric pressure value that blood vessel is placed to temperature, makes moisture content be frozen into ice crystal.
Then, adjusting environment to temperature and be-10 ℃～-25 ℃, atmospheric pressure value is 10Pa ~ 50Pa, by the distillation of ice crystal, 90% ~ 98% of above-mentioned moisture content is sloughed.Environmental temperature raises, and a part of heat is supplied with the required heat of sublimation of distillation, and a part causes the rising of blood vessel temperature.Under this atmospheric pressure value, rate of sublimation is than faster under atmospheric pressure, when the blood vessel temperature rises to distillation when consistent with above-mentioned environmental temperature, stops.
Then, adjusting environment to temperature and be 10 ℃ ~ 20 ℃, atmospheric pressure value is 10Pa ~ 50Pa, and residue moisture content is removed.Said temperature, after adjust raising, can promote to comprise the chemical bond that fettered by various gravitation and the gasification of physical bond water, but excess Temperature also can affect the biologically active of blood vessel, so temperature setting is set to 10 ℃ ~ 20 ℃.
Finally, blood vessel is kept in the environment that vacuum seal and temperature are 4 ℃ ~ 25 ℃.
The invention effect:
Of the present inventionly by adjusting temperature and the atmospheric pressure value of blood vessel environment of living in, remove the moisture content on blood vessel, preserved to greatest extent self biological structure of above-mentioned blood vessel, make it rehydration time short, performance is close to fresh blood vessel, so that clinical use; After vacuum seal, under room temperature, can preserve easily for a long time, make retain costs significantly reduce.
The accompanying drawing explanation:
Fig. 1 is the application schematic diagram of embodiment
Embodiment:
What the present embodiment was related carries out to blood vessel temperature and the atmospheric pressure value that the method for kept dry controls environment with freeze dryer, uses porcine aorta as blood vessel.
Above-mentioned freeze dryer has, for the instruction of input control temperature and atmospheric pressure value and by the guidance panel of its demonstration; Thermocouple for temperature sensor; Be used for by vacuumizing the vavuum pump of controlling atmospheric pressure value; For collecting the cold-trap of the steam got based on distillation.
Below in conjunction with accompanying drawing, the present embodiment is described:
Fig. 1 is the application schematic diagram of embodiment
At first, as shown in Fig. 11, porcine aorta is cleaned, be placed in physiological saline and soak, to maintain the stable of sample.As shown in Fig. 12, porcine aorta is cut into to the vessel segment of 15mm left and right, drain surperficial physiological saline.As shown in Fig. 13, vessel segment vertically is put in culture dish, can make like this vessel segment conduct heat abundant and even.As shown in Fig. 14, then the culture dish that will be placed with vessel segment is placed in freeze dryer on shelf, and thermocouple (in Fig. 1 7) is inserted to vessel segment.
Then as shown in Fig. 18, the guidance panel by freeze dryer (in Fig. 1 6) is set as shelf temperature-70 ℃, and pressure is atmospheric pressure.During minimum temperature that the vessel segment temperature monitored when thermocouple (in Fig. 1 7) is temperature-fall period, continue to keep one hour at this temperature, make moisture content freeze for ice crystal.
Then, as shown in Figure 9, changing shelf temperature by guidance panel (in Fig. 1 6) be-20 ℃, and opens vacuum pump switch (in Fig. 1 12) and vacuumize, until guidance panel shows that air pressure is about 10Pa.Ice crystal is distilled under extremely low pressure, and collects the steam of distillation by cold-trap (in Fig. 1 10), makes steam form windstream, and sublimation process is constantly carried out.The blood vessel temperature monitored when thermocouple (in Fig. 1 7) is identical with the shelf design temperature, finishes this step in the time of-20 ℃.
Then, as in Fig. 1 13, changing shelf temperature by guidance panel (in Fig. 1 6) is 10 ℃, and air pressure continues to maintain 10Pa.Make the vessel segment temperature raise gradually, thereby remove the residue moisture content in conjunction with water that comprises of more difficult volatilization on vessel segment.When the blood vessel temperature is 10 ℃, when identical with shelf temperature, this step finishes.
Vessel segment after drying, put into sealed bag, and normal temperature or lower temperature (4 ℃) are preserved.
Implementation result
The present embodiment is adjusted temperature and the atmospheric pressure value of blood vessel environment of living in by freeze dryer, remove the moisture content in the porcine aorta vessel segment, preserved to greatest extent self biological structure of above-mentioned vessel segment, make it rehydration time short, after vacuum seal, can preserve easily for a long time under room temperature, make retain costs significantly reduce.
Above explanation be an example of relevant blood vessel method for drying and storing, but the present invention not only only is confined to this example.
Claims (1)
1. a method of blood vessel being carried out to kept dry, then preserve for the moisture content of removing blood vessel, it is characterized in that having following steps:
The 1st step, it is under-65 ℃ ~-75 ℃, the atmospheric pressure value environment that is atmospheric pressure value that described blood vessel is placed to temperature, makes described moisture content be frozen into ice crystal,
The 2nd step, adjusting described environment to temperature is 10Pa ~ 50Pa for-10 ℃-25 ℃, atmospheric pressure value, by the distillation of described ice crystal, 90% ~ 98% of described moisture content is sloughed,
The 3rd step, adjusting described environment to temperature and be 10 ℃ ~ 20 ℃, atmospheric pressure value is 10Pa ~ 50Pa, residue moisture content is removed,
The 4th step, be kept at described blood vessel in the environment that vacuum seal and temperature are 4 ℃ ~ 25 ℃.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2012104315656A CN103416394A (en) | 2012-11-01 | 2012-11-01 | Method for drying and storage of blood vessels |
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| Application Number | Priority Date | Filing Date | Title |
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| CN2012104315656A CN103416394A (en) | 2012-11-01 | 2012-11-01 | Method for drying and storage of blood vessels |
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| CN103416394A true CN103416394A (en) | 2013-12-04 |
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| CN2012104315656A Pending CN103416394A (en) | 2012-11-01 | 2012-11-01 | Method for drying and storage of blood vessels |
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20030118982A1 (en) * | 2000-03-24 | 2003-06-26 | Naoka Yamamoto | Method of preserving tissue equivalent and tissue equivalent preserved in frozen state |
| CN1434679A (en) * | 2000-06-12 | 2003-08-06 | 苏帕契勒国际控股有限公司 | High temperature cryogenic preservation of biolcally active material |
| CN101626682A (en) * | 2006-10-27 | 2010-01-13 | 爱德华兹生命科学公司 | Biological tissue for surgical implantation |
| CN102600508A (en) * | 2012-02-14 | 2012-07-25 | 上海交通大学医学院附属上海儿童医学中心 | Porcine arterial vacuum lyophilization acellular matrix, as well as preparation method and application thereof |
-
2012
- 2012-11-01 CN CN2012104315656A patent/CN103416394A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20030118982A1 (en) * | 2000-03-24 | 2003-06-26 | Naoka Yamamoto | Method of preserving tissue equivalent and tissue equivalent preserved in frozen state |
| CN1434679A (en) * | 2000-06-12 | 2003-08-06 | 苏帕契勒国际控股有限公司 | High temperature cryogenic preservation of biolcally active material |
| CN101626682A (en) * | 2006-10-27 | 2010-01-13 | 爱德华兹生命科学公司 | Biological tissue for surgical implantation |
| CN102600508A (en) * | 2012-02-14 | 2012-07-25 | 上海交通大学医学院附属上海儿童医学中心 | Porcine arterial vacuum lyophilization acellular matrix, as well as preparation method and application thereof |
Non-Patent Citations (2)
| Title |
|---|
| 张高孟 等: "冻干血管移植", 《国外医学外科学分册》 * |
| 殷猛 等: "真空冷冻干燥过程中猪主动脉血管的力学性能", 《中国组织工程研究与临床康复》 * |
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Application publication date: 20131204 |