CN103416331B - Test device for water leaving avoiding reaction of eriocheir sinensis under environmental stress and use method thereof - Google Patents
Test device for water leaving avoiding reaction of eriocheir sinensis under environmental stress and use method thereof Download PDFInfo
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- CN103416331B CN103416331B CN201310291685.5A CN201310291685A CN103416331B CN 103416331 B CN103416331 B CN 103416331B CN 201310291685 A CN201310291685 A CN 201310291685A CN 103416331 B CN103416331 B CN 103416331B
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- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 title claims abstract description 88
- 238000012360 testing method Methods 0.000 title claims abstract description 53
- 238000000034 method Methods 0.000 title claims abstract description 21
- 230000006353 environmental stress Effects 0.000 title claims abstract description 19
- 241000371997 Eriocheir sinensis Species 0.000 title abstract description 8
- 239000003814 drug Substances 0.000 claims abstract description 64
- 239000007924 injection Substances 0.000 claims abstract description 63
- 238000002347 injection Methods 0.000 claims abstract description 63
- 229940079593 drug Drugs 0.000 claims abstract description 8
- 230000008569 process Effects 0.000 claims abstract description 7
- 241000371986 Eriocheir Species 0.000 claims description 62
- 239000012153 distilled water Substances 0.000 claims description 11
- 239000000243 solution Substances 0.000 claims description 7
- WLZRMCYVCSSEQC-UHFFFAOYSA-N cadmium(2+) Chemical compound [Cd+2] WLZRMCYVCSSEQC-UHFFFAOYSA-N 0.000 claims description 6
- 239000007788 liquid Substances 0.000 claims description 6
- 238000013459 approach Methods 0.000 claims description 4
- 238000013461 design Methods 0.000 claims description 4
- 238000002474 experimental method Methods 0.000 claims description 4
- 238000011010 flushing procedure Methods 0.000 claims description 4
- 238000002360 preparation method Methods 0.000 claims description 3
- 238000003756 stirring Methods 0.000 claims description 3
- 239000007864 aqueous solution Substances 0.000 claims description 2
- 230000009286 beneficial effect Effects 0.000 claims description 2
- 239000000747 designer drug Substances 0.000 claims description 2
- 239000000758 substrate Substances 0.000 claims description 2
- 230000035882 stress Effects 0.000 abstract description 5
- 231100000419 toxicity Toxicity 0.000 abstract description 4
- 230000001988 toxicity Effects 0.000 abstract description 4
- 230000006870 function Effects 0.000 abstract description 2
- 238000011160 research Methods 0.000 description 15
- 241000251468 Actinopterygii Species 0.000 description 9
- 239000000126 substance Substances 0.000 description 9
- 229910001385 heavy metal Inorganic materials 0.000 description 7
- 239000003905 agrochemical Substances 0.000 description 6
- 230000007613 environmental effect Effects 0.000 description 6
- IOVCWXUNBOPUCH-UHFFFAOYSA-M Nitrite anion Chemical compound [O-]N=O IOVCWXUNBOPUCH-UHFFFAOYSA-M 0.000 description 5
- 230000007059 acute toxicity Effects 0.000 description 4
- 231100000403 acute toxicity Toxicity 0.000 description 4
- XKMRRTOUMJRJIA-UHFFFAOYSA-N ammonia nh3 Chemical compound N.N XKMRRTOUMJRJIA-UHFFFAOYSA-N 0.000 description 4
- 230000008447 perception Effects 0.000 description 4
- 230000007096 poisonous effect Effects 0.000 description 4
- 239000003643 water by type Substances 0.000 description 4
- 241000238557 Decapoda Species 0.000 description 3
- 239000000356 contaminant Substances 0.000 description 3
- 230000000694 effects Effects 0.000 description 3
- 239000007789 gas Substances 0.000 description 3
- 230000004044 response Effects 0.000 description 3
- 230000004083 survival effect Effects 0.000 description 3
- 241001465754 Metazoa Species 0.000 description 2
- 229910052793 cadmium Inorganic materials 0.000 description 2
- BDOSMKKIYDKNTQ-UHFFFAOYSA-N cadmium atom Chemical compound [Cd] BDOSMKKIYDKNTQ-UHFFFAOYSA-N 0.000 description 2
- 230000008859 change Effects 0.000 description 2
- 230000007665 chronic toxicity Effects 0.000 description 2
- 231100000160 chronic toxicity Toxicity 0.000 description 2
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- 230000000366 juvenile effect Effects 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 238000012544 monitoring process Methods 0.000 description 2
- 231100000041 toxicology testing Toxicity 0.000 description 2
- 206010063659 Aversion Diseases 0.000 description 1
- 230000001154 acute effect Effects 0.000 description 1
- 238000009360 aquaculture Methods 0.000 description 1
- 230000008901 benefit Effects 0.000 description 1
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- 230000001684 chronic effect Effects 0.000 description 1
- 239000012141 concentrate Substances 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 238000004401 flow injection analysis Methods 0.000 description 1
- 238000009472 formulation Methods 0.000 description 1
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- 239000003292 glue Substances 0.000 description 1
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- 231100000567 intoxicating Toxicity 0.000 description 1
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Classifications
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
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- Testing Resistance To Weather, Investigating Materials By Mechanical Methods (AREA)
- Farming Of Fish And Shellfish (AREA)
Abstract
The invention discloses a test device for water leaving avoiding reaction of eriocheir sinensis under environmental stress. The device comprises a box body, an avoiding table arranged in the box body and an injection device laid on the bottom face of the box body and integrating functions of air injection, medicine injection and water injection. The injection device is connected with an air pump, a water storage tank and a medicine storage tank through a pipeline and a valve. A camera shooting device is hung above the box body and is connected with a computer through a video line. A use method of the test device is further disclosed. An airflow medicine injection technology is adopted, the whole medicine injection process is the medicine injection adjusting process through airflow on the outside of the box body, crab stress reaction (that the eriocheir sinensis is frightened by manual interference and runs away in all directions) caused by the fact that a user directly pours medicine into the box body is avoided, and the user can accurately judge whether the eriocheir sinensis leaves water and goes ashore because of the stress reaction or a normal toxicity avoiding strategy. Medicine can be completely dissolved into a water body quickly, evenly and completely through the pipeline, and test accuracy is ensured.
Description
Technical field
The present invention relates to testing arrangement and the using method of the dried up avoiding reaction of Eriocheir sinensia under a kind of environment-stress, be specifically related at heavy metal, the environmental contaminants such as agricultural chemicals and other water quality factor (comprise nitrite, ammonia nitrogen, salinity, PH etc.) coercing under, the testing arrangement of avoiding reaction that Eriocheir sinensia is dried up and using method, not only directly apply to the behavior reaction research field of Eriocheir sinensia under environment-stress, also be evaluate harmful substance to Eriocheir sinensia acute toxicity and chronic toxicity effect, explore Eriocheir sinensia to the ability to bear of undesired water and protection behavior, formulate the instrument that Eriocheir sinensis water water standard is indispensable.
Background technology
The height of the existence of the environmental contaminants such as heavy metal, agricultural chemicals and other water quality factor (comprising nitrite, ammonia nitrogen, salinity, PH etc.) is directly connected to and hydrobiologically grows and survive, when these envirment factors act on hydrobiological sensory system, aquatile can be made to produce corresponding behavior avoiding reaction.Aquatic animal avlidance behavior, be aquatic animal a kind of extremely responsive aversion response of producing of environmental change to external world.People often utilize this characteristic hydrobiological, so as to test aquatile to the avoidance ability of poor environment and the least concentration (also known as avoidance threshold value) that avoidance occurs for the first time thereof, be applied to the water standard of fish production water quality safety monitoring index system and formulation fishery water.
Based on fish when running into bad waters, this principle can be avoided to clear waters, having developed the avoidance experimental provision of many fish both at home and abroad, Jones(1947) devise a kind of pipeline type fish avoidance device; Hogland etc. (1973) and Wildish etc. (1977) devise one " Y " type bifurcated fish slot to avoid device; Sprague etc. (1969) devise a kind of straight type slot to avoid; Chinese aquatic science president river produces research institute and has manufactured experimently the ring-like avoidance experimental provision of a kind of P-T in 1977.
By avoiding device above, researcher to multiple fish and shrimps the avlidance behavior under toxicity stress react research (Sprague, 1965 of having carried out; Warner, 1966; Hou Lanying etc., 1993; Zhao Qian, 1997; Chen Minshan, 1999; ), meanwhile, prince strong (2006) also invented a kind of water quality safety on line early warning system based on aquatile avlidance behavior and method (granted patent).
Eriocheir sinensia is the leading kind of China's freshwater aquiculture crab class, and the behavior that relevant Eriocheir sinensia answers heavy metal and agricultural chemicals and other undesired water physical and chemical factor to coerce, Physiology and biochemistry countermeasure have been subject to the extensive concern of domestic scholars.But, research finds: different from the aquatile such as fish, the avlidance behavior that Eriocheir sinensia (crab rises since the childhood) response environment is coerced is not single avoiding to clean waters from polluted water region, especially under pond culture condition, once waters, pond is worsened, Eriocheir sinensia can show unique avlidance behavior, namely leaves Chi Shui, climbs to pond bank and avoid.So domestic and international developed fish avoiding reaction testing arrangement all can not be applied to the research of Eriocheir sinensia avoiding reaction at present.
For this reason, in the urgent need to developing a kind of avoiding reaction testing arrangement be applicable to when Eriocheir sinensia response environment is coerced.In addition, what highly pay close attention to is, the current toxicity about Eriocheir sinensia (comprises heavy metal, agricultural chemicals, nitrite, ammonia nitrogen etc.) research method all imitates in aquatiles such as other fish shrimp crabs, these research methods have obvious deficiency: in various environmentally hazardous substance to Eriocheir sinensia acute toxicity, in long term toxicity test, the crab of participating in the experiment " by force " (test crab can not be made nature and avoid) is placed in the common vessel filling trial drug solution, according to the survival of crab, grow and other some eco-physiological indexes to assess the poisonous effect of harmful substance to Eriocheir sinensia, calculate corresponding half lethal concentration (LC
50), obtain harmful substance to the safe concentration (SC of Eriocheir sinensia
50), and with the change of the physiological ecological of Eriocheir sinensia after this safe concentration or intoxicating be used as water environment monitoring, early warning index or directly instruct the therapeutic regimen of pond culture.And in fact, when harmful substance reaches " perception " concentration of Eriocheir sinensia, it just can attempt to avoid, leave water body and debark.So, crab is placed in drug solution by researcher's " by force ", this solution has probably reached " perception " concentration of crab, even far away higher than " perception " concentration of crab, in other words, in the Cultivated water or pond of reality, Eriocheir sinensia is avoided with regard to leaving the disembarkation of bad water body in this experimental concentration or lower than during this experimental concentration, crab has been placed in testing liquid by visible " by force ", reaction harmful substance that can not be real is to the poisonous effect of Eriocheir sinensia, comprise the behavior of poisoning rear Eriocheir sinensia, dead and other physiological ecological changes, the safe concentration of gained can not instruct in production.So the acute and chronic toxicological study of harmful substance to Eriocheir sinensia must be based upon on the Research foundation of avoiding reaction in water body.Avoiding reaction, as a kind of basic research, plays a key effect to Eriocheir sinensia toxicity assessment method to improving harmful substance.
In a word; design a kind of avoiding reaction testing arrangement being applicable to the Eriocheir sinensia reply poor environment factor; not only directly apply to the behavior reaction research field of Eriocheir sinensia under environment-stress; also be that research harmful substance is to Eriocheir sinensia acute toxicity and chronic toxicity effect; explore Eriocheir sinensia to the ability to bear of undesired water and protection behavior, formulate the instrument that Eriocheir sinensis water water standard is indispensable.
Summary of the invention
Technical problem to be solved by this invention is, lack the appropriate device of research Eriocheir sinensia avlidance behavior reaction and the corresponding toxicological study ways and means not problem such as science for Present Domestic, a kind of experimental rig and the using method that accurately can test the avoidance ability of Eriocheir sinensia under undesired water environment and avoid threshold value are provided.It is high that the present invention has measurement sensitivity, can reach the requirement of every experimental index, functional, and cost is low, easy to operate applicable, is easy to promote.
In order to solve the problem, the technical solution adopted in the present invention is as follows:
The testing arrangement of the dried up avoiding reaction of Eriocheir sinensia under environment-stress, comprising:
One casing;
Be arranged on the avoidance platform in described casing, described avoidance platform has certain height apart from the case bottom surface of described casing;
The case bottom surface being laid on described casing has the injection device of gas injection, injection and water-filling function concurrently;
One air pump be connected with described injection device by the first pipeline; Described first pipeline is serially connected with the first valve, the second valve and the 3rd valve, a four-way connection is connected in series with between the second valve at described first valve, wherein the first valve is connected with the second interface with the first interface of the second valve respectively at described four-way connection, and the 3rd valve is connected in series with between the second valve;
One water tank, described water tank is provided with gas injection port and delivery port, described gas injection port by serial connection second pipe and the 4th valve be connected on the first pipeline between described air pump and the first valve, described delivery port by be connected in series the 3rd pipeline and the 5th valve be connected on the 3rd interface of described four-way connection;
One medicine storing pot, described medicine storing pot is provided with air intake and medicine outlet, described air intake by serial connection the 4th pipeline and the 6th valve be connected on the 4th interface of described four-way connection; Described medicine outlet is connected on the described 3rd the first pipeline between valve and injection device by the 5th pipeline of serial connection and the 7th valve;
One is suspended on the camera head observed above described casing and to the situation in described casing;
One computer, the video signal input terminal in described computer is connected with described camera head.
In a preferred embodiment of the invention, described camera head is by a they sup-port above described casing, and described support bar is fixed on described casing.
In a preferred embodiment of the invention, described avoidance platform is trapezoidal avoidance platform, is arranged on the case bottom surface of described casing, and the end face of this trapezoidal avoidance platform is plane.
In a preferred embodiment of the invention, described injection device is formed by becoming groined type to arrange also mutually through pvc pipe, and described pvc pipe offers some apertures.
In a preferred embodiment of the invention, described water tank offers water filling port; Described medicine storing pot offers medicine-pouring port.
In a preferred embodiment of the invention, the bottom of described casing offers delivery port.
Under described environment-stress, the concrete steps of the using method of the testing arrangement of the dried up avoiding reaction of Eriocheir sinensia are as follows:
(1) in described casing, inject a certain amount of pure water, make water level be no more than the end face of described avoidance platform;
(2) the 4th valve is closed to the 7th valve, open the first valve to the 3rd valve, now the air-flow that produces of air pump through the first pipeline and approach first valve, the second valve and the 3rd valve arrive described casing case bottom injection device in, and being injected in the water body in described casing by injection device, air Injection process stirs the water body in described casing slightly;
(3) Eriocheir sinensia of participating in the experiment is put into casing, start to support temporarily and tame, Eriocheir sinensia adapted to and is familiar with the living environment in described casing, comprising the position of being familiar with avoiding platform, be beneficial to duration of test crab and can find rapidly avoidance region;
(4) support temporarily and tame after 24 hours, according to the drug solution of test requirements document preparation variable concentrations gradient, and injecting medicine storing pot, in water tank, fill it up with distilled water simultaneously;
(5) the 6th valve and the 7th valve is opened, now, in the injection device of air pump air-flow out on the one hand bottom the case that the first pipeline and the first valve, the second valve and the 3rd valve arrive described casing, water body in casing is stirred, on the other hand in approach first pipeline, the first valve, four-way connection, the 4th pipeline and the 6th valve press-in medicine storing pot, liquid in medicine storing pot is poured in injection device by medicine outlet, the 5th pipeline, the 7th valve and the 3rd valve, the first pipeline, and is injected in the water body in described casing by injection device, pour completely when the liquid in medicine storing pot after in the water body in casing, close the second valve and the 3rd valve at once, and open rapidly the 4th valve and the 5th valve and close the 6th valve, now, air pump air-flow is out through the first pipeline, 4th valve, second pipe and be pressed in water tank, by the distilled water in water tank by delivery port, 3rd pipeline, 5th valve, second valve, first pipeline and the 3rd valve pour in injection device, and be injected in the water body in described casing by injection device, the main purpose of this step is the medicine that use distilled water flushing remains in pipeline, guarantee that medicine all to arrive in casing and to be dissolved in water body, learn through gathering aqueous solution test in casing, after use distilled water flushing, concentration of cadmium ions in casing basic consistent with former design concentration (positive and negative be no more than 1%).
(6) after injection 2 hours by camera head and computer online record in the quantity of avoiding Eriocheir sinensia on platform, and further mathematical statistics, the relatively rate of avoidance of Eriocheir sinensia under different pharmaceutical concentration, tests its avoidance ability to the poor environment factor;
(7) according to result of study, further designer drug substrate concentration, gropes the drug concentration that Eriocheir sinensia makes avoidance for the first time, the least concentration namely avoided, also known as avoidance threshold value.
Liquid of the present invention can be heavy metal, agricultural chemicals, nitrite, PH regulator solution etc.
Owing to have employed technical scheme as above, the present invention adopts air-flow injection technology, whole injection process moves towards regulating medicine injection process by air-flow outside casing, can avoid directly causing crab class stress reaction (artificial disturbance crab is got a fright and flee in all directions) toward perfusion in casing because of artificial, cause accurately judging that crab is because of stress reaction or the dried up disembarkation normally kept away caused by malicious strategy, and medicine is dissolved in water body by pipeline fast, uniformly, completely, guarantee the accuracy of testing.
Concentration is also known as avoidance thresholds
Accompanying drawing illustrates:
Fig. 1 is the structural representation of the testing arrangement of the dried up avoiding reaction of Eriocheir sinensia under environment-stress of the present invention.
Fig. 2 is the structural representation of injection device.
Embodiment
The present invention is further described below see accompanying drawing.
See Fig. 1 and Fig. 2, under the environment-stress provided in figure, the testing arrangement of the dried up avoiding reaction of Eriocheir sinensia comprises a casing, this casing is for material makes an aquarium 100 with the plastic plate of grey, the specification of aquarium 100 is that 0.6m(is long) × 0.4m(is wide) × 0.3m(is high), offer a delivery port 130 in a side 110 of aquarium 100 with bottom surface 120 intersection, control draining by a screw switch 140.
Be that the pvc pipe 230 of 0.5 centimetre connects into groined type and forms an injection device 200 by diameter, this injection device 200 is long is 0.58m, wide is 0.38m, this injection device 200 wherein a plane are pvc pipe 230 bores 16 holes 210, the aperture in each hole 210 is 0.3cm, afterwards injection device 200 is layed at the bottom of the case of aquarium 100, is fixed with marine glue.Except injection device 200 pvc pipe 230 occupied by aquarium 100 bottom surface except, aquarium 100 bottom surface remainder lays white plastic plate 220, the thickness of white plastic plate 220 is consistent with the diameter of pvc pipe 230, is 0.5cm, makes the bottom surface of whole aquarium 100 substantially smooth;
Take diameter as the reinforcing bar of 0.5cm be support, be welded into a halfpace 310, the length and width of halfpace 310 bottom surface are respectively 0.4m and 0.3m, and the length and width of end face are respectively 0.2m and 0.15m, and halfpace 310 height is 0.25m.Screen of screen window 320 is all entirely placed in 6 faces of halfpace 310, and is fixed, and namely completes the making avoiding platform 300.The avoidance platform 300 made is positioned in aquarium 100, between two parties.
Prepare a medicine storing pot 400 and a standpipe 500, the top of medicine storing pot 400 offers medicine-pouring hole 410 and air intake 420, and bottom offers medicine outlet 430, and medicine-pouring hole 410 is furnished with stopple.
The top of water tank 500 offers injecting hole 520 and water filling port 510, and bottom offers delivery port 530, and water injection hole 510 is furnished with stopple.
Injection device 200 connects air pump 700 by a pipeline 610, and on pipeline 610, be serially connected with valve 620,630,640 and four-way connection 650, wherein a joint of four-way connection 650 connects valve 620, another joint connects valve 630, and valve 640 is serially connected between valve 630 and injection device 200.
The injecting hole 520 at water tank 500 top is connected on the pipeline 610 between air pump 700 and valve 620 by pipeline 660 and valve 670, and the delivery port 530 bottom water tank 500 is connected on four-way connection 650 the 3rd joint by pipeline 680 and valve 690.
The medicine-pouring hole 410 at medicine storing pot 400 top is connected on four-way connection 650 the 4th joint by pipeline 710 and valve 720, and the medicine outlet 430 bottom medicine storing pot 400 is connected on the pipeline 610 between valve 640 and injection device 200 by pipeline 730 and valve 740.
By a metallic support rod 810, camera 820 is suspended on the top of aquarium 100, camera 820 is connected by the video signal input terminal of video line with computer 830, just can be observed the situation in aquarium 100 like this by camera 820 and computer 830.Metallic support rod 810 can directly be fixed on the tank wall of water tank case 100, also can fix in addition.
The test of the dried up avoiding reaction of Eriocheir sinensia under environment-stress is used for for the testing arrangement of the dried up avoiding reaction of Eriocheir sinensia under preparation 18 above-mentioned environment-stress.
The test of Eriocheir sinensia avlidance behavior
For introducing the use of avlidance behavior testing arrangement, present case will test the avlidance behavior of Juvenile Crab Eriocheir sinensis (about body weight 2g) under variable concentrations (0mg/L, 0.01mg/L, 0.05mg/L, 0.25mg/L, 0.5mg/L, 1mg/L) cadmium ion poisonous effect, and concrete testing procedure is:
(1) in the aquarium 100 of 18 testing arrangements, respectively add 50L pure water, respectively put into Juvenile Crab Eriocheir sinensis 20 that is healthy, specification close (2 ± 0.1g) simultaneously.
(2) valve 670, valve 690, valve 720, the valve 740 in 18 testing arrangements is closed, open valve 620, valve 630, valve 640, the air-flow that now air pump 700 of 18 testing arrangements produces enters into injection device 200 through piping 610, valve 620, four-way connection 650, valve 630, valve 640, and enter in aquarium 100 by the hole 210 on the pvc pipe 230 in injection device 200, finally be dissolved in the water body in aquarium 100, whole air Injection process stirs water body slightly.
After (3) 24 hours, 18 testing arrangements are divided into 6 groups at random, every test concentrations 3 repetition, prepare the medicine concentrate (concentration of 6 groups is respectively 0mg/L, 25mg/L, 125mg/L, 625mg/L, 1.25g/L, 2.5g/L) of each concentration group respectively, the medicine that namely should add in 50L water is all dissolved in the pure water of 20ml and is configured to concentrated medicament, and concentrated medicament is injected the medicine storing pot 400 of corresponding testing arrangement respectively by the medicine-pouring hole 410 at medicine storing pot 400 top in 18 testing arrangements.Meanwhile, in water tank 500, distilled water (capacity is 100ml) is filled it up with by the water filling port 510 at water tank 500 top in 18 testing arrangements.
(4) valve 720 in 18 testing arrangements, valve 740 is opened, now, the air-flow of air pump 700 enters into the injection device 200 of 18 testing arrangements on the one hand by pipeline 610, valve 620, four-way connection 650, valve 630, valve 640, and enters in aquarium 100 by the hole 210 on the pvc pipe 230 in injection device 200; Medicine storing pot 400 is entered on the other hand by four-way connection 650, pipeline 710, valve 720, concentrated medicament in medicine storing pot 400 is extruded by the medicine outlet 430 bottom medicine storing pot 400, the concentrated medicament extruded enters into the injection device 200 of 18 testing arrangements more rapidly by pipeline 730, valve 740, valve 640 and pipeline 610, and enter in aquarium 100 by the hole 210 on the pvc pipe 230 in injection device 200, be dissolved in uniformly in the water body of aquarium 100.After the concentrated medicament in the medicine storing pot 400 in 18 testing arrangements has flowed substantially, valve-off 630 and valve 640 at once, and open rapidly valve 670, valve 690 and valve-off 620, now air-flow is through pipeline 610, pipeline 660, valve 670 enters into water tank 500, distilled water in water tank 500 is extruded by the delivery port 530 bottom water tank 500, the distilled water extruded is by pipeline 680, valve 690, four-way connection 650, valve 720, pipeline 710 enters into medicine storing pot 400, again by the medicine outlet 430 bottom medicine storing pot 400, pipeline 730, valve 740, valve 640 and pipeline 610 enter into injection device 200 rapidly, the main purpose of this step is the medicine that use purified rinse water remains in pipeline, guarantee that medicine all to arrive in aquarium and to be dissolved in water body, after tested, after use purified rinse water, cadmium ion in aquarium basic consistent with former design concentration (positive and negative be no more than 1%).
(5) determine through test of many times, after injection, in 2 hours, Eriocheir sinensia avoiding reaction is basicly stable, so after 2 hours, the quantity of crab on the avoidance platform 300 recorded is taken by camera 820, statistics Eriocheir sinensia makes the percentage of dried up avoidance under the poisonous effect of above variable concentrations cadmium ion, and result is as table 1.
Table 1
(6) result of study is obtained:
1. adopt probability unit-mass concentration logarithm return law of the straight line, obtain regression equation: y=20.001x+89.057. researcher can according to the avoidance protection situation of Eriocheir sinensia under this equation calculating variable concentrations cadmium ion.
2. use above-mentioned equation, the least concentration (i.e. the first threshold value that avoiding reaction occurs) calculating Eriocheir sinensia avoidance under the coercing of heavy metal cadmium for this example of 0.014mg/L(altogether 20 crabs participate in the experiment, occur avoiding when namely referring to that rate of avoidance is 5% for the first time).
3. previous researcher (Li Wenyan etc., 2008) research cadmium to concentration set during Eriocheir sinensia acute toxicity experience far away higher than " perception " concentration of Eriocheir sinensia, namely set concentration truly can not reflect the survival condition of crab, and the safe concentration obtained also loses meaning.Confirm further at this, research Eriocheir sinensia environmental resistance ability (survival condition) must consider that it can dried uply carry out avoiding this fact.Meanwhile, suggestion can use apparatus of the present invention to carry out envirment factor from now on and coerce research to Eriocheir sinensia, better authenticity.
More than show and describe general principle of the present invention and principal character and advantage of the present invention.The technical staff of the industry should understand; the present invention is not restricted to the described embodiments; what describe in above-described embodiment and specification just illustrates principle of the present invention; without departing from the spirit and scope of the present invention; the present invention also has various changes and modifications; be applicable to the avoidance capability study that various environmental factors coerces (comprising the water quality factor such as the environmental contaminants such as each heavy metal species, agricultural chemicals and ammonia nitrogen, nitrite, PH, salinity) lower Eriocheir sinensia, these changes and improvements all fall in the claimed scope of the invention.Application claims protection domain is defined by appending claims and equivalent thereof and equivalent method.
Claims (7)
1. the testing arrangement of the dried up avoiding reaction of Eriocheir sinensia under environment-stress, is characterized in that, comprising:
One casing;
Be arranged on the avoidance platform in described casing, described avoidance platform has certain height apart from the case bottom surface of described casing;
The case bottom surface being laid on described casing has the injection device of gas injection, injection and water-filling function concurrently;
One air pump be connected with described injection device by the first pipeline; Described first pipeline is serially connected with the first valve, the second valve and the 3rd valve, a four-way connection is connected in series with between the second valve at described first valve, wherein the first valve is connected with the second interface with the first interface of the second valve respectively at described four-way connection, and the 3rd valve is connected in series with between the second valve;
One water tank, described water tank is provided with gas injection port and delivery port, described gas injection port by serial connection second pipe and the 4th valve be connected on the first pipeline between described air pump and the first valve, described delivery port by be connected in series the 3rd pipeline and the 5th valve be connected on the 3rd interface of described four-way connection;
One medicine storing pot, described medicine storing pot is provided with air intake and medicine outlet, described air intake by serial connection the 4th pipeline and the 6th valve be connected on the 4th interface of described four-way connection; Described medicine outlet is connected on the described 3rd the first pipeline between valve and injection device by the 5th pipeline of serial connection and the 7th valve;
One is suspended on the camera head observed above described casing and to the situation in described casing;
One computer, the video signal input terminal in described computer is connected with described camera head.
2. the testing arrangement of the dried up avoiding reaction of Eriocheir sinensia under environment-stress as claimed in claim 1, it is characterized in that, described camera head is by a they sup-port above described casing, and described support bar is fixed on described casing.
3. the testing arrangement of the dried up avoiding reaction of Eriocheir sinensia under environment-stress as claimed in claim 1, it is characterized in that, described avoidance platform is trapezoidal avoidance platform, is arranged on the case bottom surface of described casing, and the end face of this trapezoidal avoidance platform is plane.
4. the testing arrangement of the dried up avoiding reaction of Eriocheir sinensia under environment-stress as claimed in claim 1, is characterized in that, described injection device, by becoming groined type to arrange and mutually through pvc pipe is formed, described pvc pipe offers some apertures.
5. the testing arrangement of the dried up avoiding reaction of Eriocheir sinensia under environment-stress as claimed in claim 1, is characterized in that, described water tank offers water filling port; Described medicine storing pot offers medicine-pouring port.
6. the testing arrangement of the dried up avoiding reaction of Eriocheir sinensia under environment-stress as claimed in claim 1, it is characterized in that, the bottom of described casing offers delivery port.
7. the using method of the testing arrangement of the dried up avoiding reaction of Eriocheir sinensia under environment-stress described in any one of claim 1 to 6 claim, it is characterized in that, the concrete steps of this using method are as follows:
(1) in described casing, inject a certain amount of pure water, make water level be no more than the end face of described avoidance platform;
(2) the 4th valve is closed to the 7th valve, open the first valve to the 3rd valve, now the air-flow that produces of air pump through the first pipeline and approach first valve, the second valve and the 3rd valve arrive described casing case bottom injection device in, and being injected in the water body in described casing by injection device, air Injection process stirs the water body in described casing slightly;
(3) Eriocheir sinensia of participating in the experiment is put into casing, start to support temporarily and tame, Eriocheir sinensia adapted to and is familiar with the living environment in described casing, comprising the position of being familiar with avoiding platform, be beneficial to duration of test crab and can find rapidly avoidance region;
(4) support temporarily and tame after 24 hours, according to the drug solution of test requirements document preparation variable concentrations gradient, and injecting medicine storing pot, in water tank, fill it up with distilled water simultaneously;
(5) the 6th valve and the 7th valve is opened, now, air pump air-flow is out on the one hand by way of the first pipeline and the first valve, in injection device bottom the case that second valve and the 3rd valve arrive described casing, water body in casing is stirred, approach first pipeline on the other hand, first valve, four-way connection, 4th pipeline and the 6th valve are pressed in medicine storing pot, by the liquid in medicine storing pot by medicine outlet, 5th pipeline, 7th valve and the 3rd valve, first pipeline pours in injection device, and be injected in the water body in described casing by injection device, pour completely when the liquid in medicine storing pot after in the water body in casing, close the second valve and the 3rd valve at once, and open rapidly the 4th valve and the 5th valve and close the 6th valve, now, air pump air-flow is out through the first pipeline, 4th valve, second pipe and be pressed in water tank, by the distilled water in water tank by delivery port, 3rd pipeline, 5th valve, second valve, first pipeline and the 3rd valve pour in injection device, and be injected in the water body in described casing by injection device, the main purpose of this step is the medicine that use distilled water flushing remains in pipeline, guarantee that medicine all to arrive in casing and to be dissolved in water body, learn through gathering aqueous solution test in casing, after use distilled water flushing, concentration of cadmium ions in casing is positive and negative is no more than former design concentration 1%,
(6) after injection 2 hours by camera head and computer online record in the quantity of avoiding Eriocheir sinensia on platform, and further mathematical statistics, the relatively rate of avoidance of Eriocheir sinensia under different pharmaceutical concentration, tests its avoidance ability to the poor environment factor;
(7) according to result of study, further designer drug substrate concentration, gropes the drug concentration that Eriocheir sinensia makes avoidance for the first time, the least concentration namely avoided, also known as avoidance threshold value.
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| CN201414341Y (en) * | 2009-04-17 | 2010-03-03 | 中国水产科学研究院东海水产研究所 | Measuring device for substrate color selection of goby larval fish |
| CN201504488U (en) * | 2009-04-17 | 2010-06-16 | 中国水产科学研究院东海水产研究所 | Gobioid larval fish secrecy measuring device |
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| CN103004676A (en) * | 2012-12-27 | 2013-04-03 | 中国水产科学研究院淡水渔业研究中心 | Experimental device for determining fish stress response to water flow |
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