CN103411981B - One utilizes dielectric and magnetic characteristic to differentiate the epithelioglandular method of gastric cancer - Google Patents

Abstract

The invention discloses one utilizes dielectric and magnetic characteristic to differentiate the epithelioglandular method of gastric cancer, it is characterized as being after including respectively normal person's gastric gland epithelium cell sample and gastric cancer glandular epithelium sample are carried out Cell culture invitro, through trypsinization, piping and druming, it is configured to the step of corresponding cell suspending liquid；Above-mentioned cell suspending liquid is carried out dielectric spectra measurement, acquisition normal person's gastric gland epithelium cell and gastric cancer glandular epithelium have the dielectric spectra characteristic parameter of Dielectric behavior feature, analyzed by significance test of difference, it is thus achieved that differentiate the step with dielectric spectra characteristic parameter and respective value scope；Measure the discriminating dielectric spectra characteristic ginseng value of gastric cells suspension to be measured, if any of which discriminating dielectric spectra characteristic ginseng value drops in corresponding gastric cancer group numerical range and outside normal group numerical range, can differentiate that this gastric cells is gastric cancer glandular epithelium, advantage is non-invasi, accuracy is high, provides the foundation for developing early gastric cancer sieving and diagnosis instrument further。

Description

One utilizes dielectric and magnetic characteristic to differentiate the epithelioglandular method of gastric cancer

Technical field

The present invention relates to stomach cancer cell discrimination method, especially relate to one and utilize dielectric and magnetic characteristic to differentiate the epithelioglandular method of gastric cancer。

Background technology

Gastric cancer is the modal malignant tumor of China, and its M & M all occupies first of various malignant tumor。China's gastric cancer mortality belongs to world's high mortality level, and male is 4.2～7.9 times of European and American developed countries, and women is 3.8～8.0 times。Therefore, gastric cancer is the emphasis of prevention and treatment of malignant tumors, actively develops rationale and the clinical application research of gastric cancer, discloses its pathogenesis, finds effective diagnoses and treatment, have important practical significance。Being presently available for auxiliary stomach cancer diagnostic method: endoscope's (gastroscope) mirror and pathological evaluation, exfoliative cytology inspection, x-ray canel barium meal contrast examination, ultrasound diagnosis, CT scan, nuclear magnetic resonance MRI checks, Serum Pepsinogen (PGI, PGII) detection etc.。Wherein, gastroscopy is clinical the most frequently used stomach cancer diagnostic method, it relies primarily on Visual Observations Observations gastric mucosa metamorphosis, to high doubt region, by gastroscope, suspect tissue biopsy being sampled, then carry out pathological diagnosis, current pathological diagnosis is still goldstandard, but have the disadvantage in that 1) rely on morphopathology to change as diagnostic criteria, tumor has evolved to middle and advanced stage；2) biopsy has damaging。

Therefore, Chinese scholars is all exploring various ways and meanses, finds early gastric caacer diagnostic method and non-damage diagnostic techniques。Wherein, changing according to stomach organization cell electrical characteristics, assist diagnosis to be exactly a kind of selection the early stage of tumor cell, it main reason is that histiocyte electrology characteristic changes early than its morphological change。Therefore, gastric cancer glandular epithelium electrical characteristic parameter change is explored, by the characteristic parameter of these changes, it is achieved early gastric caacer is assisted the monitoring of diagnosis and treatment index, it has also become the focus of bio electricity engineering and biomedical engineering's concern and difficulties。At present, both at home and abroad but without openly any about the correlational study report utilizing dielectric and magnetic characteristic to differentiate the epithelioglandular method of gastric cancer。

Summary of the invention

The technical problem to be solved is to provide a kind of non-invasi, and what accuracy was high utilizes dielectric and magnetic characteristic to differentiate the epithelioglandular method of gastric cancer, provides the foundation for developing early gastric cancer sieving and diagnosis instrument further。

This invention address that the technical scheme that above-mentioned technical problem adopts is: one utilizes dielectric and magnetic characteristic to differentiate the epithelioglandular method of gastric cancer, comprises the following steps:

(1) sample collection

After normal person's gastric gland epithelium cell sample and gastric cancer glandular epithelium sample are carried out Cell culture invitro respectively, through trypsinization, piping and druming, it is configured to corresponding normal gastric glandular epithelium suspension and gastric cancer glandular epithelium suspension, treats impedance measurement；

(2) dielectric spectra is measured

Normal gastric glandular epithelium suspension and gastric cancer glandular epithelium suspension are injected separately in measuring cell, measuring cell is connected on electric impedance analyzer, 80 Frequency points are taken in 0.01～100MHz frequency range, set each Frequency point Automatic Cycle scanning survey 3 times, constant voltage is 200mV, measure the electric capacity C and conductance G of the cell sample of each Frequency point, convert the electric capacity C under each frequency and conductance G to the DIELECTRIC CONSTANT ε under corresponding frequencies and electrical conductivity κ；

(3) discriminating foundation is set up

By the dielectric constant frequency spectrum to normal person's gastric gland epithelium cell sample Yu gastric cancer glandular epithelium sample, electrical conductivity frequency spectrum, complex number plane figure, dielectric loss factor frequency spectrum, the analysis of electrical conductivity imaginary part frequency spectrum and dielectric loss angle tangent frequency spectrum, acquisition normal person's gastric gland epithelium cell and gastric cancer glandular epithelium have the dielectric spectra characteristic parameter of Dielectric behavior feature, normal person's gastric gland epithelium cell and gastric cancer epithelioglandular dielectric spectra characteristic parameter are analyzed by statistical significance test of difference, obtain the dielectric spectra characteristic parameter between normal person's gastric gland epithelium cell and gastric cancer glandular epithelium with significant difference, namely discriminating dielectric spectra characteristic parameter is obtained, and by dielectric constant frequency spectrum, electrical conductivity frequency spectrum, complex number plane figure, dielectric loss factor frequency spectrum, electrical conductivity imaginary part frequency spectrum and dielectric loss angle tangent frequency spectrum, obtain the numerical range of normal person's gastric gland epithelium Identification cell lines numerical range with dielectric spectra characteristic parameter and gastric cancer glandular epithelium discriminating dielectric spectra characteristic parameter；

(4) stomach cancer cell differentiates

Take after gastric cells to be measured carries out Cell culture invitro, through trypsinization, piping and druming, it is configured to gastric cells suspension to be measured, measure the discriminating dielectric spectra characteristic ginseng value of gastric cells suspension to be measured, if any of which discriminating dielectric spectra characteristic ginseng value drops in the numerical range of corresponding gastric cancer glandular epithelium discriminating dielectric spectra characteristic parameter and outside the numerical range of normal person's gastric gland epithelium Identification cell lines dielectric spectra characteristic parameter, can differentiate that this gastric cells is gastric cancer glandular epithelium。

Cell culture invitro process described in step (1) is: be placed in by cell sample in 1640 culture medium containing 10% inactivated fetal bovine serum, 100IU/mL penicillin and 100IU/mL streptomycin, at 37 DEG C, containing the CO of 5%₂Incubator in growth。

The actual conditions that dielectric spectra described in step (2) is measured is: after electric impedance analyzer start preheating 30min, 0.1mol/LKCL standard solution is used to be corrected, then the fixture of cell measurement is connected, 80 Frequency points are taken in 0.01～100MHz frequency range, set ac-excited signal source voltage 200mV, measuring the electric capacity C and conductance G of each cell sample, the scanning of each Frequency point Automatic Cycle carries out 3 measurements and takes its average；Measuring cell is mainly constituted by transparent with machine glass tubing and platinum Pt electrode, is measuring electrode arranged above with a miniature sample holes, and electrode specification is: diameter 7.25mm, spacing 8.5mm, volume is about 0.4ml；Using 0.1mol/LKCL standard solution to be corrected measuring electrode, at 25 ± 1 DEG C by measuring acquisition measured data electric capacity C and conductance G and measuring cell constant Co=0.016737pF, swim electric capacity C₁=1.81pF, has then corrected measured data:

Electric capacity after correction: $\mathrm{Cs}=\frac{C(1+{\mathrm{\ω}}^2\mathrm{LC})+{\mathrm{LG}}^2}{{(1+{\mathrm{\ω}}^2\mathrm{LC})}^2+{\left(\mathrm{\ωLG}\right)}^2}-C_1$ (formula 1)

Conductance after correction: $\mathrm{Gs}=\frac{G}{{(1+{\mathrm{\ω}}^2\mathrm{LC})}^2+{\left(\mathrm{\ωLG}\right)}^2}$ (formula 2)

Wherein electrode inductance coefficent L=1.26nH, angular frequency=2 π f, f are electric field frequency, DIELECTRIC CONSTANT ε=C_S/ Co and electrical conductivity κ=(ε_v/ Co) * Gs, permittivity of vacuum ε_v=8.854pF/m。

Dielectric spectra characteristic parameter described in step (3) includes high frequency limit dielectric constant, low-frequency limit dielectric constant, maximum dielectric increment constant, electrical conductivity low-frequency pole limitation, electrical conductivity high frequency limit amount, maximum conductivity increment, fisrt feature frequency, second feature frequency, third feature frequency, dielectric loss factor peak, f₂Electrical conductivity imaginary part peak, f₃Electrical conductivity imaginary part peak and dielectric loss angle tangent。

Discriminating dielectric spectra characteristic parameter described in step (3) includes electrical conductivity low-frequency pole limitation, electrical conductivity high frequency limit amount, maximum conductivity increment, fisrt feature frequency, second feature frequency, third feature frequency, dielectric loss factor peak and f₂Electrical conductivity imaginary part peak。

The numerical range of the normal person's gastric gland epithelium Identification cell lines dielectric spectra characteristic parameter described in step (3) and the numerical range of described gastric cancer glandular epithelium discriminating dielectric spectra characteristic parameter are as shown in table 1 below:

Table 1

The gastric cancer group that represents * compares P < 0.05, * * * with Normal group, and to represent that gastric cancer group compares P < 0.001, n with Normal group be sample size。

Compared with prior art, it is an advantage of the current invention that: one of the present invention utilizes dielectric and magnetic characteristic to differentiate the epithelioglandular method of gastric cancer, owing to people's gastric gland epithelium cell strain is different with people's gastric cancer glandular epithelium strain cell interior structure, its dielectric constant and electrical conductivity also each differ, when DC Electric Field is in cell, the transfer of the interface restriction electric charge of different structure causes charge accumulated, there occurs polarization phenomena between the two media electrically differed, polarization phenomena are prevalent in biological cell, dielectric spectra method is utilized to it will be seen that cell dielectric behavior characteristics, differentiate gastric cancer glandular epithelium, the method non-invasi, accuracy is high, early stage for realizing gastric cancer better is detected, grasp the pathological changes mechanism of gastric cancer glandular epithelium electrical characteristics, and for realizing experiment in vivo further and exploitation has the sieving and diagnosis instrument of independent intellectual property rights and establishes experiment basis。

Accompanying drawing explanation

Fig. 1 is normal person's gastric gland epithelium cell and the epithelioglandular dielectric constant frequency spectrum of people's gastric cancer；

Fig. 2 is normal person's gastric gland epithelium cell and the epithelioglandular electrical conductivity frequency spectrum of people's gastric cancer；

Fig. 3 is normal person's gastric gland epithelium cell and the epithelioglandular dielectric constant complex number plane figure of people's gastric cancer；

Fig. 4 is normal person's gastric gland epithelium cell and the epithelioglandular electrical conductivity complex number plane figure of people's gastric cancer；

Fig. 5 is normal person's gastric gland epithelium cell and the epithelioglandular dielectric loss factor frequency spectrum of people's gastric cancer；

Fig. 6 is normal person's gastric gland epithelium cell and the epithelioglandular electrical conductivity imaginary part frequency spectrum of people's gastric cancer；

Fig. 7 is normal person's gastric gland epithelium cell and the epithelioglandular dielectric loss angle tangent frequency spectrum of people's gastric cancer。

Detailed description of the invention

Below in conjunction with accompanying drawing embodiment, the present invention is described in further detail。

Specific embodiment

One, experiment material

Normal person gastric gland epithelium cell strain GES-1, purchased from Shanghai Inst. of Life Science, CAS cell bank, sample size is 11；

People gastric cancer glandular epithelium strain MGC-803, purchased from Shanghai Inst. of Life Science, CAS cell bank, sample size is 8；

0.9%NaCl normal saline (Xi'an company limited of Double-Crane Pharmaceutical Co., Ltd), hyclone (hyclone), dual anti-(hyclone), 1640 culture medium (hyclone), 75% ethanol (Yong Feng chemical plant, Zhenghai, Ningbo City)

Two, experimental technique

Normal person gastric gland epithelium cell strain GES-1 and people gastric cancer glandular epithelium strain MGC-803 has the structures such as extracellular fluid, cell membrane, Cytoplasm, nuclear membrane, cell caryoplasm and entoblast。Owing to cell interior structure is different, its dielectric constant and electrical conductivity also each differ。When DC Electric Field is in cell, the transfer of the interface restriction electric charge of different structure causes charge accumulated, there occurs polarization phenomena between the two media electrically differed, and polarization phenomena are prevalent in biological cell。Dielectric spectra method is utilized to it will be seen that cell dielectric behavior characteristics。The main contents of this experiment are through the dielectric spectra to MGC-803 and GES-01 and detect, and then analyze both difference, thus realizing the early stage detection of gastric cancer better。

1 normal person gastric gland epithelium cell line GES-1 experiment

1.1GES-1 cell is cultivated

Being placed in by normal person gastric gland epithelium cell GES-1 containing 10% inactivated fetal bovine serum, 100IU/mL penicillin, cultivate in 1640 culture medium of 100IU/mL streptomycin, cell is maintained at 37 DEG C, containing 5%CO₂Incubator in growth, through trypsinization, piping and druming, prepare into suspension cell liquid, and be configured to the cell suspending liquid of different specific volume。

The measurement of 1.2GES-1 dielectric spectra

Take GES-1 cell suspending liquid 0.4ml, pipet is used to inject in measuring cell by cell suspending liquid, measuring cell is connected on electric impedance analyzer, arranging measuring condition is measure 80 Frequency points in 0.01～100MHz frequency, given constant voltage is 200mV, measure the electric capacity C and conductance G of the cell sample of each Frequency point, record experimental data。

1.3GES-1 specific volume is measured

Take out GES-1 cell sample from measuring cell after impedance measurement, take trace and inject specific volume of cell pipe, use centrifuge, with 10000rpm, centrifugal 5min, and charge to specific volume of cell。

2 gastric cancer glandular epithelium strain MGC-803 experiments

2.1MGC-803 cell is cultivated

Gastric cancer glandular epithelium strain MGC-803 is placed in containing 10% inactivated fetal bovine serum, 100IU/mL penicillin, cultivates in 1640 culture medium of 100IU/mL streptomycin。Cell is maintained at 37 DEG C, 5%CO₂Incubator grows, through trypsinization, piping and druming, prepares into suspension cell liquid, and be configured to the cell suspending liquid of different specific volume。

The measurement of 2.2MGC-803 dielectric spectra

Take MGC-803 cell suspending liquid 0.4ml, use pipet to inject in measuring cell by cell suspending liquid, measuring cell is connected on electric impedance analyzer。Arranging measuring condition is measure 80 Frequency points in 0.01～100MHz frequency, and given voltage is 200mV。Measure the electric capacity C and conductance G of the cell sample of each Frequency point, record experimental data。

2.3MGC-803 specific volume is measured

Take out MGC-803 cell sample from measuring cell after impedance measurement, take trace and inject specific volume of cell pipe, use centrifuge, with 12000rpm, centrifugal 5min, and charge to specific volume of cell。

2.4 measuring conditions set

At temperature is (25 ± 1) DEG C, after 4294A type electric impedance analyzer start preheating 30min, it is corrected, is then connected the fixture of cell measurement, used the correction of 0.1mol/LKCL standard solution。Taking 80 Frequency points in 0.01～100MHz frequency range, set ac-excited signal source voltage 200mV, measure the electric capacity C and conductance G of each cell sample, the scanning of each Frequency point Automatic Cycle carries out 3 measurements and takes its average；Measuring cell is mainly constituted by transparent with machine glass tubing and Pt electrode, at measuring cell arranged above with a miniature sample holes。Electrode specification is: diameter 7.25mm, spacing 8.5mm, volume is about 0.4ml；Using 0.1mol/LKCL standard solution to be corrected measuring electrode, at 25 ± 1 DEG C by measuring acquisition measured data and measuring cell constant Co=0.016737pF, swim electric capacity C₁=1.81pF, has then corrected measured data:

Electric capacity after correction: $\mathrm{Cs}=\frac{C(1+{\mathrm{\&omega;}}^2\mathrm{LC})+{\mathrm{LG}}^2}{{(1+{\mathrm{\&omega;}}^2\mathrm{LC})}^2+{\left(\mathrm{\&omega;LG}\right)}^2}-C_1$ (formula 1)

Conductance after correction: $\mathrm{Gs}=\frac{G}{{(1+{\mathrm{\&omega;}}^2\mathrm{LC})}^2+{\left(\mathrm{\&omega;LG}\right)}^2}$ (formula 2)

Wherein electrode inductance coefficent L=1.26nH, angular frequency=2 π f, f are electric field frequency, according to ε=C_S/ Co and κ=(ε_v/ Co) Gs converts DIELECTRIC CONSTANT ε and the electrical conductivity κ of different frequency, permittivity of vacuum ε to_v=8.854pF/m。

Three, experimental result

By the dielectric constant frequency spectrum to normal person gastric gland epithelium cell GES-1 Yu gastric cancer glandular epithelium MGC-803, the comprehensive analysis of electrical conductivity frequency spectrum, complex number plane figure, dielectric loss factor frequency spectrum, electrical conductivity imaginary part frequency spectrum and dielectric loss angle tangent frequency spectrum, determine that normal gastric glandular epithelium and gastric cancer glandular epithelium have the dielectric spectra characteristic parameter of Dielectric behavior feature, be specifically shown in Fig. 1-Fig. 7 analysis (following analysis data result is sample average):

As it is shown in figure 1, MGC-803 and GES-01 compares, within the scope of 0.01-100MHz, the limit DIELECTRIC CONSTANT ε of gastric cancer glandular epithelium (MGC-803)_LDecline 2.85%, low-frequency limit DIELECTRIC CONSTANT ε_hDecline 3.07%, but not significant difference between ε spectral line。Its medium dielectric constant microwave medium frequency spectrum, i.e. complex dielectric constant (ε^*) with the electric field frequency f functional relationships ε changed^*=ε-jκ/(2πfε_v)=ε '-ε ", real part of permittivity ε '=ε, imaginary part of dielectric constant ε "=κ/ω ε_v, angular frequency=2 π f。

As in figure 2 it is shown, MGC-803 compares than GES-01, the electrical conductivity κ curve of gastric cancer glandular epithelium (MGC-803) is comprehensively upper to be moved, and reflection MGC-803 cell electric conductivity increases, wherein electrical conductivity low-frequency pole limitation κ_LIncrease 13.19%(P < 0.001) and electrical conductivity high frequency limit amount κ_hIncrease 10.32%(P < 0.001)。Wherein electrical conductivity frequency spectrum, i.e. plural number electrical conductivity (κ^*) it is the function of frequency f: κ^*=κ+jωεε_v=κ '+κ ", electrical conductivity real part κ '=κ, electrical conductivity imaginary part κ "=ε ω ε_v。

As shown in Figure 3, MGC-803 and GES-1 compares, the complex number plane figure main manifestations of gastric cancer glandular epithelium (MGC-803) is that curve circular arc reduces to the left, shows as dielectric constant increment Delta ε and reduces 2.85% thus causing that area reduces, fisrt feature frequency f₁Increase 28%(P < 0.001)。Its medium dielectric constant microwave medium complex number plane figure, namely abscissa is real part of permittivity ε '=ε, and vertical coordinate is imaginary part of dielectric constant increment Delta ε "=(κ-κ_L)/(2πfε_v)

As shown in Figure 4, MGC-803 and GES-1 compares, and the complex number plane figure of gastric cancer glandular epithelium (MGC-803) is overall to right translation, and main manifestations is electrical conductivity low-frequency pole limitation κ_LIncrease 13.19%(P < 0.001) and electrical conductivity high frequency limit amount κ_hIncrease 10.32%(P < 0.001) make the monolithic conductive performance enhancement of MGC-803。In characteristic frequency, f₂Increase 41.38%(P < 0.001) and f₃Reduce 11.04%(P < 0.001), characteristic frequency also there occurs change。Wherein electrical conductivity complex number plane figure, namely abscissa is electrical conductivity real part κ '=κ, and vertical coordinate is electrical conductivity imaginary part increment Delta κ "=(ε-ε_L)×(2πfε_v)。

As it is shown in figure 5, dielectric loss factor Δ ε " in frequency spectrum, MGC-803 and GES-1 compares, the Δ ε of MGC-803 " frequency spectrum is depressed, and shows as peak delta ε "_pReduce 14.89%(P < 0.001), the amplitude at peak becomes round and smooth, illustrates that MGC-803 reduces than normal cell dielectric loss。Wherein dielectric loss factor frequency spectrum, namely dielectric loss factor (Δ ε "=(κ-κ_L)/(2πfε_v)) with electric field frequency change function。

As shown in Figure 6, electrical conductivity imaginary part Δ κ " in frequency spectrum, MGC-803 and GES-01 compares, and on the left of the electrical conductivity imaginary part curve of MGC-803, peak region is raised substantially, its peak value (P₂=Δκ”_p(f₂)) increase 16.67%(P < 0.001), second feature frequency f₂Increase 41.38%(P < 0.001), right side main peak (P₃=Δκ”_p(f₃)) increasing by 2.67%, corresponding Frequency point is the 3rd characteristic frequency f₃Reduce 11.04(P < 0.001)。Wherein electrical conductivity imaginary part frequency spectrum, namely electrical conductivity imaginary part increment (Δ κ "=(ε-ε_L)×(2πfε_v)) with electric field frequency change function。

As it is shown in fig. 7, in dielectric loss angle tangent Δ tg δ frequency spectrum, MGC-803 and GES-01 compares, the dielectric loss angle tangent maximum Δ tg δ of MGC-803_pCompare with GES-1 and there was no significant difference。Wherein dielectric loss angle tangent frequency spectrum, the i.e. dielectric loss angle tangent (function that Δ tg δ=Δ ε "/ε ') changes with electric field frequency。

By normal gastric gland epithelium cell and gastric cancer epithelioglandular dielectric spectra characteristic parameter are carried out statistical significance test analysis, determine the dielectric spectra characteristic parameter between normal gastric glandular epithelium with gastric cancer glandular epithelium with significant difference and the numerical range of corresponding dielectric spectra characteristic parameter, it is specifically shown in table 1

Table 1GES-1 and MGC-803 electrophysiological characteristics parameter

Note: * * represents and gastric cancer group compares P < 0.05, * * * with Normal group to represent that gastric cancer group compares P < 0.001, n with Normal group be sample size。

As seen from the above table, electrical conductivity low-frequency pole limitation, electrical conductivity high frequency limit amount, maximum conductivity increment, fisrt feature frequency, second feature frequency, third feature frequency, dielectric loss factor peak and f₂Electrical conductivity imaginary part peak can as differentiating the epithelioglandular dielectric spectra characteristic parameter of gastric cancer。

After gastric cells to be measured is carried out Cell culture invitro, through trypsinization, piping and druming, it is configured to gastric cells suspension to be measured, measures the electrical conductivity low-frequency pole limitation of gastric cells suspension to be measured, electrical conductivity high frequency limit amount, maximum conductivity increment, fisrt feature frequency, second feature frequency, third feature frequency, dielectric loss factor peak and f₂The size at electrical conductivity imaginary part peak, if any of which discriminating dielectric spectra characteristic ginseng value falls in the numerical range of corresponding gastric cancer glandular epithelium discriminating dielectric spectra characteristic parameter in table 1 above and outside the numerical range of normal person's gastric gland epithelium Identification cell lines dielectric spectra characteristic parameter, can differentiate that this gastric cells is gastric cancer glandular epithelium。The foundation of people's gastric cancer glandular epithelium dielectric spectra characteristic parameter and numerical range size thereof, for the electrology characteristic research that gastric cancer is diseases related, it is provided that new research means and evaluation index, has potential potential applicability in clinical practice。

Described above is not limitation of the present invention, and the present invention is also not limited to the example above。Those skilled in the art in the essential scope of the present invention, change, remodeling, interpolation or the replacement made, also should belong to protection scope of the present invention, protection scope of the present invention is as the criterion with claims。

Claims (1)

1. differentiate normal gastric glandular epithelium and the gastric cancer glandular epithelium method for building up of dielectric spectra characteristic parameter, it is characterized in that specifically comprising the following steps that the dielectric constant frequency spectrum by normal person's gastric gland epithelium cell sample Yu gastric cancer glandular epithelium sample, electrical conductivity frequency spectrum, complex number plane figure, dielectric loss factor frequency spectrum, the analysis of electrical conductivity imaginary part frequency spectrum and dielectric loss angle tangent frequency spectrum, acquisition normal person's gastric gland epithelium cell and gastric cancer glandular epithelium have the dielectric spectra characteristic parameter of Dielectric behavior feature, normal person's gastric gland epithelium cell and gastric cancer epithelioglandular dielectric spectra characteristic parameter are analyzed by statistical significance test of difference, obtain the dielectric spectra characteristic parameter between normal person's gastric gland epithelium cell and gastric cancer glandular epithelium with significant difference, namely discriminating dielectric spectra characteristic parameter is obtained, and by dielectric constant frequency spectrum, electrical conductivity frequency spectrum, complex number plane figure, dielectric loss factor frequency spectrum, electrical conductivity imaginary part frequency spectrum and dielectric loss angle tangent frequency spectrum, obtain the numerical range of normal person's gastric gland epithelium Identification cell lines numerical range with dielectric spectra characteristic parameter and gastric cancer glandular epithelium discriminating dielectric spectra characteristic parameter, the actual conditions that described dielectric spectra is measured is: after electric impedance analyzer start preheating 30min, 0.1mol/LKCL standard solution is used to be corrected, then the fixture of cell measurement is connected, 80 Frequency points are taken in 0.01～100MHz frequency range, set ac-excited signal source voltage 200mV, measure the electric capacity C and conductance G of each cell sample, the scanning of each Frequency point Automatic Cycle carries out 3 measurements and takes its average；Measuring cell is mainly constituted by transparent with machine glass tubing and platinum electrode, is measuring electrode arranged above with a miniature sample holes, and electrode specification is: diameter 7.25mm, spacing 8.5mm, volume is about 0.4ml；Using 0.1mol/LKCL standard solution to be corrected measuring electrode, at 25 ± 1 DEG C by measuring acquisition measured data electric capacity C and conductance G and measuring cell constant Co=0.016737pF, swim electric capacity C₁=1.81pF, has then corrected measured data:

Electric capacity after correction: $Cs=\frac{C(1+{\mathrm{\&omega;}}^{2}LC)+{\mathrm{LG}}^2}{{(1+{\mathrm{\&omega;}}^{2}LC)}^2+{\left(\mathrm{\&omega;}LG\right)}^2}-C_1$ (formula 1)

Conductance after correction: $Gs=\frac{G}{{(1+{\mathrm{\&omega;}}^{2}LC)}^2+{\left(\mathrm{\&omega;}LG\right)}^2}$ (formula 2)

Wherein electrode inductance coefficent L=1.26nH, angular frequency=2 π f, f are electric field frequency, DIELECTRIC CONSTANT ε=C_S/ Co and electrical conductivity κ=(ε_v/ Co) * Gs, permittivity of vacuum ε_v=8.854pF/m, the described numerical range differentiating normal gastric glandular epithelium dielectric spectra characteristic parameter and the numerical range of described discriminating gastric cancer glandular epithelium dielectric spectra characteristic parameter are as shown in the table:

* P < 0.05, * * * P < 0.001 compares with matched group, and n is sample size。