CN103411962B - A kind of cobalt ions colorimetric determination kit and detection method thereof - Google Patents
A kind of cobalt ions colorimetric determination kit and detection method thereof Download PDFInfo
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- CN103411962B CN103411962B CN201310179630.5A CN201310179630A CN103411962B CN 103411962 B CN103411962 B CN 103411962B CN 201310179630 A CN201310179630 A CN 201310179630A CN 103411962 B CN103411962 B CN 103411962B
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- 229910001429 cobalt ion Inorganic materials 0.000 title claims abstract description 56
- XLJKHNWPARRRJB-UHFFFAOYSA-N cobalt(2+) Chemical compound [Co+2] XLJKHNWPARRRJB-UHFFFAOYSA-N 0.000 title claims abstract description 56
- 238000001514 detection method Methods 0.000 title claims abstract description 22
- 239000000243 solution Substances 0.000 claims abstract description 69
- XQSBLCWFZRTIEO-UHFFFAOYSA-N hexadecan-1-amine;hydrobromide Chemical compound [Br-].CCCCCCCCCCCCCCCC[NH3+] XQSBLCWFZRTIEO-UHFFFAOYSA-N 0.000 claims abstract description 56
- PCHJSUWPFVWCPO-UHFFFAOYSA-N gold Chemical compound [Au] PCHJSUWPFVWCPO-UHFFFAOYSA-N 0.000 claims abstract description 37
- UIIMBOGNXHQVGW-UHFFFAOYSA-M Sodium bicarbonate Chemical compound [Na+].OC([O-])=O UIIMBOGNXHQVGW-UHFFFAOYSA-M 0.000 claims abstract description 35
- BTBUEUYNUDRHOZ-UHFFFAOYSA-N Borate Chemical compound [O-]B([O-])[O-] BTBUEUYNUDRHOZ-UHFFFAOYSA-N 0.000 claims abstract description 26
- 239000007853 buffer solution Substances 0.000 claims abstract description 25
- ZNNZYHKDIALBAK-UHFFFAOYSA-M potassium thiocyanate Chemical compound [K+].[S-]C#N ZNNZYHKDIALBAK-UHFFFAOYSA-M 0.000 claims abstract description 22
- 235000017557 sodium bicarbonate Nutrition 0.000 claims abstract description 18
- 229910000030 sodium bicarbonate Inorganic materials 0.000 claims abstract description 17
- MHAJPDPJQMAIIY-UHFFFAOYSA-N Hydrogen peroxide Chemical compound OO MHAJPDPJQMAIIY-UHFFFAOYSA-N 0.000 claims abstract description 12
- 229940116357 potassium thiocyanate Drugs 0.000 claims abstract description 11
- 238000002156 mixing Methods 0.000 claims description 17
- 238000000034 method Methods 0.000 claims description 14
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 14
- 238000010790 dilution Methods 0.000 claims description 12
- 239000012895 dilution Substances 0.000 claims description 12
- 238000001556 precipitation Methods 0.000 claims description 10
- 229910017052 cobalt Inorganic materials 0.000 claims description 9
- 239000010941 cobalt Substances 0.000 claims description 9
- GUTLYIVDDKVIGB-UHFFFAOYSA-N cobalt atom Chemical compound [Co] GUTLYIVDDKVIGB-UHFFFAOYSA-N 0.000 claims description 9
- UIIMBOGNXHQVGW-DEQYMQKBSA-M Sodium bicarbonate-14C Chemical compound [Na+].O[14C]([O-])=O UIIMBOGNXHQVGW-DEQYMQKBSA-M 0.000 claims description 7
- 239000011435 rock Substances 0.000 claims description 7
- 238000012360 testing method Methods 0.000 claims description 6
- 238000002360 preparation method Methods 0.000 claims description 5
- 238000006243 chemical reaction Methods 0.000 claims description 3
- 230000001105 regulatory effect Effects 0.000 claims description 2
- 238000012455 bioassay technique Methods 0.000 abstract description 2
- 239000012472 biological sample Substances 0.000 abstract 1
- 238000006555 catalytic reaction Methods 0.000 description 6
- OUUQCZGPVNCOIJ-UHFFFAOYSA-M Superoxide Chemical compound [O-][O] OUUQCZGPVNCOIJ-UHFFFAOYSA-M 0.000 description 5
- 235000012206 bottled water Nutrition 0.000 description 5
- 239000003153 chemical reaction reagent Substances 0.000 description 5
- 239000003651 drinking water Substances 0.000 description 5
- 238000005530 etching Methods 0.000 description 5
- 238000000862 absorption spectrum Methods 0.000 description 3
- 238000004458 analytical method Methods 0.000 description 3
- 238000001095 inductively coupled plasma mass spectrometry Methods 0.000 description 3
- 238000003756 stirring Methods 0.000 description 3
- CIWBSHSKHKDKBQ-JLAZNSOCSA-N Ascorbic acid Chemical compound OC[C@H](O)[C@H]1OC(=O)C(O)=C1O CIWBSHSKHKDKBQ-JLAZNSOCSA-N 0.000 description 2
- 238000010521 absorption reaction Methods 0.000 description 2
- 230000015572 biosynthetic process Effects 0.000 description 2
- 239000000872 buffer Substances 0.000 description 2
- 238000004737 colorimetric analysis Methods 0.000 description 2
- 229910052737 gold Inorganic materials 0.000 description 2
- 239000010931 gold Substances 0.000 description 2
- 239000007788 liquid Substances 0.000 description 2
- 239000002245 particle Substances 0.000 description 2
- SQGYOTSLMSWVJD-UHFFFAOYSA-N silver(1+) nitrate Chemical compound [Ag+].[O-]N(=O)=O SQGYOTSLMSWVJD-UHFFFAOYSA-N 0.000 description 2
- 238000003786 synthesis reaction Methods 0.000 description 2
- 238000004832 voltammetry Methods 0.000 description 2
- JPVYNHNXODAKFH-UHFFFAOYSA-N Cu2+ Chemical compound [Cu+2] JPVYNHNXODAKFH-UHFFFAOYSA-N 0.000 description 1
- 235000000069 L-ascorbic acid Nutrition 0.000 description 1
- 239000002211 L-ascorbic acid Substances 0.000 description 1
- SZZRMCOLWUGEGL-UHFFFAOYSA-M N#C[O-].N#CO.N#CO.S.[K+] Chemical compound N#C[O-].N#CO.N#CO.S.[K+] SZZRMCOLWUGEGL-UHFFFAOYSA-M 0.000 description 1
- SJUCACGNNJFHLB-UHFFFAOYSA-N O=C1N[ClH](=O)NC2=C1NC(=O)N2 Chemical compound O=C1N[ClH](=O)NC2=C1NC(=O)N2 SJUCACGNNJFHLB-UHFFFAOYSA-N 0.000 description 1
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 description 1
- 206010047571 Visual impairment Diseases 0.000 description 1
- 229960005070 ascorbic acid Drugs 0.000 description 1
- 230000008033 biological extinction Effects 0.000 description 1
- 229910021538 borax Inorganic materials 0.000 description 1
- KGBXLFKZBHKPEV-UHFFFAOYSA-N boric acid Chemical compound OB(O)O KGBXLFKZBHKPEV-UHFFFAOYSA-N 0.000 description 1
- 239000004327 boric acid Substances 0.000 description 1
- 230000000711 cancerogenic effect Effects 0.000 description 1
- 231100000315 carcinogenic Toxicity 0.000 description 1
- FDJOLVPMNUYSCM-WZHZPDAFSA-L cobalt(3+);[(2r,3s,4r,5s)-5-(5,6-dimethylbenzimidazol-1-yl)-4-hydroxy-2-(hydroxymethyl)oxolan-3-yl] [(2r)-1-[3-[(1r,2r,3r,4z,7s,9z,12s,13s,14z,17s,18s,19r)-2,13,18-tris(2-amino-2-oxoethyl)-7,12,17-tris(3-amino-3-oxopropyl)-3,5,8,8,13,15,18,19-octamethyl-2 Chemical compound [Co+3].N#[C-].N([C@@H]([C@]1(C)[N-]\C([C@H]([C@@]1(CC(N)=O)C)CCC(N)=O)=C(\C)/C1=N/C([C@H]([C@@]1(CC(N)=O)C)CCC(N)=O)=C\C1=N\C([C@H](C1(C)C)CCC(N)=O)=C/1C)[C@@H]2CC(N)=O)=C\1[C@]2(C)CCC(=O)NC[C@@H](C)OP([O-])(=O)O[C@H]1[C@@H](O)[C@@H](N2C3=CC(C)=C(C)C=C3N=C2)O[C@@H]1CO FDJOLVPMNUYSCM-WZHZPDAFSA-L 0.000 description 1
- 239000003086 colorant Substances 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 239000000470 constituent Substances 0.000 description 1
- 229910001431 copper ion Inorganic materials 0.000 description 1
- 238000010168 coupling process Methods 0.000 description 1
- 238000013016 damping Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 238000004090 dissolution Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 239000012467 final product Substances 0.000 description 1
- 239000012530 fluid Substances 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- FDWREHZXQUYJFJ-UHFFFAOYSA-M gold monochloride Chemical compound [Cl-].[Au+] FDWREHZXQUYJFJ-UHFFFAOYSA-M 0.000 description 1
- 238000001727 in vivo Methods 0.000 description 1
- QSHDDOUJBYECFT-UHFFFAOYSA-N mercury Chemical compound [Hg] QSHDDOUJBYECFT-UHFFFAOYSA-N 0.000 description 1
- 229910052753 mercury Inorganic materials 0.000 description 1
- 229910021645 metal ion Inorganic materials 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 230000003647 oxidation Effects 0.000 description 1
- 238000007254 oxidation reaction Methods 0.000 description 1
- 230000001590 oxidative effect Effects 0.000 description 1
- 210000002381 plasma Anatomy 0.000 description 1
- 231100000614 poison Toxicity 0.000 description 1
- 230000007096 poisonous effect Effects 0.000 description 1
- 229910052700 potassium Inorganic materials 0.000 description 1
- 239000011591 potassium Substances 0.000 description 1
- 238000011084 recovery Methods 0.000 description 1
- 229910001961 silver nitrate Inorganic materials 0.000 description 1
- 229910000033 sodium borohydride Inorganic materials 0.000 description 1
- 239000012279 sodium borohydride Substances 0.000 description 1
- 239000004328 sodium tetraborate Substances 0.000 description 1
- 235000010339 sodium tetraborate Nutrition 0.000 description 1
- 239000003053 toxin Substances 0.000 description 1
- 231100000765 toxin Toxicity 0.000 description 1
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- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
- Investigating Or Analyzing Non-Biological Materials By The Use Of Chemical Means (AREA)
Abstract
The present invention relates to environment and bioassay technique field, specifically a kind of cobalt ions colorimetric determination kit and detection method thereof.Kit comprises, hydrogen peroxide, potassium rhodanide, gold nanorods solution, standard color comparison card F and color comparison tube molten containing cetyl ammonium bromide (CTAB) borate buffer solution, sodium bicarbonate; Described containing cetyl ammonium bromide (CTAB) borate buffer solution, pH is 7.4-9.6, and concentration is 10-200mM; Described sodium bicarbonate solution concentration is 0.01-1M; Described H
2o
2solution concentration is 0.01-1M; Described potassium thiocyanate solution concentration is 0.01-1M.Utilize the present invention to detect cobalt ions, have clever lightness high, selectivity is good, and fast, naked eyes such as easily to observe at the advantage, under the experiment condition optimized, can realize the detection of environment and biological sample cobalt ions.
Description
Technical field
The present invention relates to environment and bioassay technique field, specifically a kind of cobalt ions colorimetric determination kit and detection method thereof.
Background technology
Cobalt ions is as one of biosome indispensable element, act as very important status in vivo, it is one of constituent of cobalamin. may the research of this reason to the detection of cobalt ions be not very concerned just, but, when it was reported that the bright cobalt ions finite concentration when in blood plasma, it will cause some serious diseases.Cobalt and his compound have been noted may be become aetology toxin and have carcinogenic tendency.In addition, cobalt ions, as a metal ion species, can not be decomposed, and thus has the effect of biological concentration and biomagnification, so more adds the risk of the food chain top mankind, is thus necessary to the detection of cobalt ions.
What traditional method detected cobalt ions has atomic absorption spectrum (AAS), inductively coupled plasma mass spectrometry coupling technique (ICP-MS), voltammetry etc.AAS, ICP-MS need large-scale instrument, not only expensive and be not suitable for Site Detection, and voltammetry is relatively simple and fast, but often need poisonous mercury film electrode or need complicated chemically modified electrode.Relative to above method, colorimetric analysis advantage is that its naked eyes are easy to observation, is very suitable for on-the-spot detecting in real time, simple to operate and low price.Golden nanometer particle is widely used in colorimetric analysis in recent years, this is the molar extinction coefficient making it good by golden nanometer particle surface plasmon absorption, the present invention utilizes cobalt ions catalysis generation superoxide radical etching gold nanorods to achieve highly sensitive, the object of quick detection cobalt ions, in this way detect the method for cobalt ions, domesticly at present to have not been reported.
Summary of the invention
The object of the invention is to provide a kind of cobalt ions colorimetric determination kit and detection method thereof.
For achieving the above object, the technical solution used in the present invention is:
A kind of cobalt ions colorimetric determination kit, kit comprises that cetyl ammonium bromide (CTAB) borate buffer solution, sodium bicarbonate are molten, hydrogen peroxide, potassium rhodanide, gold nanorods solution, standard color comparison card F and color comparison tube;
Described containing cetyl ammonium bromide (CTAB) borate buffer solution, pH is 7.4-9.6, and concentration is 10-200mM;
Described sodium bicarbonate solution concentration is 0.01-1M;
Described H
2o
2solution concentration is 0.01-1M;
Described potassium thiocyanate solution concentration is 0.01-1M.
Described is 0.1-20mM containing CTAB concentration in cetyl ammonium bromide (CTAB) borate buffer solution.
Further, containing cetyl ammonium bromide (CTAB) borate buffer solution pH in described kit is 9.0, and concentration is 50mM, and the concentration containing CTAB is 2mM; The length-diameter ratio of gold nanorods solution to be concentration be 2.3nM is the gold nanorods solution of 2:1; Sodium bicarbonate solution concentration is 0.1M; H
2o
2solution concentration is 0.1M; Potassium thiocyanate solution concentration is 0.1M.
Gold nanorods solution is turned the/centrifugal 5-30min of min at 6000-10000, and collecting precipitation also with intermediate water dilution, turns/centrifugal the 5-30min of min with 5000-9000 after dilution again, and precipitation is again with intermediate water dilution, stand-by.
The method of the detection copper ion of cobalt ions colorimetric determination kit, by testing sample color comparison tube, add borate buffer solution containing CTAB, add sodium bicarbonate, H
2o
2rock mixing with potassium rhodanide, add gold nanorods solution again after mixing, shake up; Then at 60-100 DEG C of temperature, hatch 2-20 minute, observe color by naked eyes or ultraviolet-visual spectrometer, contrast with standard color comparison card F and determine cobalt ions content.
Further, the borate buffer solution of testing sample containing CTAB is regulated pH, and keeps final volume to be 800-900 μ L, add sodium bicarbonate, H
2o
2rock mixing with potassium rhodanide, add the gold nanorods solution of 200-300 μ L after mixing again, shake up; Then at 60-100 DEG C of temperature, hatch 2-20 minute, observe color by naked eyes or ultraviolet-visual spectrometer, contrast with standard color comparison card F and determine cobalt ions content.
The preparation of described standard color comparison card F is, gets 0-300nM variable concentrations cobalt standard color range solution and is positioned over respectively in color comparison tube, add borate buffer solution, sodium bicarbonate, the H containing CTAB
2o
2rock mixing with potassium rhodanide, after mixing, add gold nanorods again, shake up; Then at 50-100 DEG C of temperature, hatch 2-30 minute, carry out chromogenic reaction, use professional camera record colour developing result, after gathering picture, form cobalt standard color comparison card F with the picture by color gradient.
Described containing cetyl ammonium bromide (CTAB) borate buffer solution, pH is 7.4-9.6, and concentration is 10-200mM; Described sodium bicarbonate solution concentration is 0.01-1M; Described H
2o
2solution concentration is 0.01-1M; Described potassium thiocyanate solution concentration is 0.01-1M.
Further, described is 9.0 containing cetyl ammonium bromide (CTAB) borate buffer solution pH, and concentration is 50mM, and the concentration containing CTAB is 2mM; The length-diameter ratio of gold nanorods solution to be concentration be 2.3nM is the gold nanorods solution of 2:1; Sodium bicarbonate solution concentration is 0.1M; H
2o
2solution concentration is 0.1M; Potassium thiocyanate solution concentration is 0.1M.
Gold nanorods solution is turned the/centrifugal 5-30min of min at 6000-10000, and collecting precipitation also with intermediate water dilution, turns/centrifugal the 5-30min of min with 5000-9000 after dilution again, and precipitation is again with intermediate water dilution, stand-by.
Principle (see figure 1) of the present invention is containing in the borate buffer solution of sodium bicarbonate, cobalt ions catalyzing hydrogen peroxide produces the superoxide radical with strong oxidizing property, thus accelerate the oxidation dissolution of gold nanorods its radial absorption spectrum generation blue shift (see figure 3) is changed along with obvious color, this change and concentration of cobalt ions are proportionate (see figure 5).
The advantage that the present invention has: the present invention utilizes cobalt ions catalysis to etch gold nanorods colorimetric determination cobalt ions, have highly sensitive (detect and be limited to 1.0nM), selectivity good (see Fig. 4), speed is fast, do not need instrument, be applicable to the advantages such as on-the-spot detection in real time.
Accompanying drawing explanation
Fig. 1 is the principle that cobalt ions catalysis provided by the invention produces superoxide radical etching gold nanorods.
Fig. 2 be cobalt ions catalysis provided by the invention produce superoxide radical etching gold nanorods before and after images of transmissive electron microscope, wherein cobalt ions catalysis produce superoxide radical etching gold nanorods before (left side) afterwards (right side).
Fig. 3 is the abosrption spectrogram after cobalt ions provided by the invention (concentration from low to high) catalysis etching gold nanorods.
Fig. 4 is the selectivity experimental result of detection cobalt ions provided by the invention and corresponding photo.
Fig. 5 is standard color comparison card C provided by the invention.
Fig. 6 is detection cobalt ions process flow diagram provided by the invention.
Fig. 7 is the cobalt ions range of linearity in detection potable water provided by the invention.
Fig. 8 is cobalt ions (mark-on) design sketch in detection potable water provided by the invention.
Embodiment
Embodiment 1: in potable water, cobalt ions (mark-on) detects
Kit comprises that borate buffer, sodium bicarbonate are molten, hydrogen peroxide, potassium rhodanide, gold nanorods solution, standard color comparison card F and color comparison tube containing cetyl ammonium bromide (CTAB);
Described borate buffer solution be with analytically pure borax and boric acid intermediate water formulated, the pH of buffer after preparation is 9.0, and concentration is 50mM; Add the CTAB of high concentration again, make CTAB concentration in damping fluid be 2mM;
Described sodium bicarbonate solution concentration is 0.1M, is dissolved in intermediate water obtains by analysis pure sodium bicarbonate;
Described H
2o
2solution concentration is 0.1M, is diluted obtain by the pure 30% hydrogen peroxide intermediate water of analysis;
Described potassium thiocyanate solution concentration is 0.1M, is dissolved in intermediate water obtains by analysis bright sulfur potassium cyanate.Preparation containing cetyl ammonium bromide (CTAB) gold nanorods solution:
1) gold nanorods obtains as follows:
Synthesis gold seeds liquid: be added to by chlorauric acid solution in cetyl ammonium bromide (CTAB) solution and stir, add iced sodium borohydride again in the mixed liquor stirred, make mixed liquor by glassy yellow to purplish grey, then at 26 DEG C, leaves standstill 2 hours, stand-by;
Nanometer rods is synthesized: add 1200 μ L gold chloride (50mM) solution and stir and evenly mix containing in cetyl ammonium bromide (CTAB) solution of 0.1M to 100mL, add silver nitrate (0.01M) the solution mixing of 300 μ L, add 960 μ L ascorbic acid (0.1M) solution subsequently, now solution is become colorless by yellowish-brown, finally add 200 μ L gold seeds liquid (first step synthesis), about 10-20 minute, solution colour has the five colors to gradually become blue-green, stop stirring, leave standstill after 20 hours and use.The radial absorption peak of uv-visible absorption spectra figure is shown in figure tri-abosrption spectrogram 0 at 670nM(), according to langbobier law, the concentration estimating nanometer rods is 2.3nM.
2) gold nanorods solution needed for this method obtains: synthetic gold nanorods is turned the/centrifugal 5-30min of min at 6000-10000, collecting precipitation also dilutes with intermediate water, turn/centrifugal the 5-30min of min with 5000-9000 again after dilution, precipitation with intermediate water dilution, to obtain final product again.
The preparation of described standard color comparison card F is, get 10 μ L concentration and be respectively 0,5,40,50,60,100,300nM cobalt standard color range solution, joins and is equipped with in the color comparison tube of 800 μ L containing the borate buffer solution of CTAB, shake up, then add 25 μ L sodium bicarbonates, 20 μ LH respectively
2o
2rock mixing with 7.5 μ L potassium rhodanides, add the gold nanorods containing CTAB after mixing again, shake up; Then at 95 DEG C of temperature, hatch 6 minutes, carry out chromogenic reaction, use professional camera record colour developing result, after gathering picture, form cobalt standard color comparison card F(Fig. 5 with the picture by color gradient).
I.e. kit:
Borate buffer solution, pH is 9.0 concentration is 0.2M;
Sodium bicarbonate solution, concentration is 0.1M;
H
2o
2solution, concentration is 0.1M;
Potassium thiocyanate solution, concentration is 0.1M;
Color comparison tube, 1.5mL;
Glass scales suction pipe;
Cobalt standard color range solution;
Cobalt standard color comparison card.
In potable water, cobalt ions (mark-on) detects
(1) get 3 1.5mL color comparison tubes, add in every color comparison tube and pipette 200 μ L borate buffer solutions (0.2M) from reagent bottle A, then add sodium bicarbonate in 25 μ L reagent bottle B successively, H in 20 μ L reagent bottle C
2o
2with potassium rhodanide in 7.5 μ L reagent bottle D, mixing, then adds containing three kinds of variable concentrations cobalt ions (5nM, 50nM respectively, 100nM) mark-on sample 600 μ L, fully after mixing, mark 1,2, No. 3, then add the gold nanorods 200 μ L containing CTAB in E reagent bottle respectively, shake up.
(2) at 95 DEG C of temperature, 6 minutes are hatched.
(3) observe color change (as Fig. 8), color comparison tube and standard color comparison card F(be shown in Fig. 5) compare, determine the concentration range of potable water mark-on sample cobalt ions, can find out very consistent with colorimetric card concentration correspondence; Outside can passing through, visible ray spectrogram obtains peak shift simultaneously, and according to the determination content of typical curve determination cobalt ions, the recovery of standard addition of this method testing result at 95-102%(in table 1), describe the reliability of the practical application of this method.
Table 1 cobalt ions mark-on test result
Claims (10)
1. a cobalt ions colorimetric determination kit, is characterized in that: kit comprises containing cetyl ammonium bromide (CTAB) borate buffer solution, sodium bicarbonate solution, hydrogen peroxide, potassium rhodanide, gold nanorods solution, standard color comparison card F and color comparison tube;
Described containing cetyl ammonium bromide (CTAB) borate buffer solution, pH is 7.4-9.6, and concentration is 10-200mM;
Described sodium bicarbonate solution concentration is 0.01-1M;
Described H
2o
2solution concentration is 0.01-1M;
Described potassium thiocyanate solution concentration is 0.01-1M.
2. by cobalt ions colorimetric determination kit according to claim 1, it is characterized in that: described is 0.1-20mM containing CTAB concentration in cetyl ammonium bromide (CTAB) borate buffer solution.
3. by the cobalt ions colorimetric determination kit described in claim 1 or 2, it is characterized in that: containing cetyl ammonium bromide (CTAB) borate buffer solution pH in described kit is 9.0, and concentration is 50mM, and the concentration containing CTAB is 2mM; The length-diameter ratio of gold nanorods solution to be concentration be 2.3nM is the gold nanorods solution of 2:1; Sodium bicarbonate solution concentration is 0.1M; H
2o
2solution concentration is 0.1M; Potassium thiocyanate solution concentration is 0.1M.
4. by cobalt ions colorimetric determination kit according to claim 1, it is characterized in that: gold nanorods solution is turned the/centrifugal 5-30min of min at 6000-10000, collecting precipitation also dilutes with intermediate water, / centrifugal the 5-30min of min is turned with 5000-9000 again after dilution, precipitation is again with intermediate water dilution, stand-by.
5. a method for the detection cobalt ions of cobalt ions colorimetric determination kit according to claim 1, is characterized in that: by testing sample color comparison tube, add borate buffer solution containing cetyl ammonium bromide (CTAB), add sodium bicarbonate, H
2o
2rock mixing with potassium rhodanide, add gold nanorods solution again after mixing, shake up; Then at 60-100 DEG C of temperature, hatch 2-20 minute, observe color by naked eyes or ultraviolet-visual spectrometer, contrast with standard color comparison card F and determine cobalt ions content.
6. by the method for the detection cobalt ions of cobalt ions colorimetric determination kit according to claim 5, it is characterized in that: the borate buffer solution of testing sample containing cetyl ammonium bromide (CTAB) is regulated pH, and keep final volume to be 800-900 μ L, add sodium bicarbonate, H
2o
2rock mixing with potassium rhodanide, add the gold nanorods solution of 200-300 μ L after mixing again, shake up; Then at 60-100 DEG C of temperature, hatch 2-20 minute, observe color by naked eyes or ultraviolet-visual spectrometer, contrast with standard color comparison card F and determine cobalt ions content.
7. by the method for the detection cobalt ions of the cobalt ions colorimetric determination kit described in claim 5 or 6, it is characterized in that: the preparation of described standard color comparison card F is, get 0-300nM variable concentrations cobalt standard color range solution to be positioned over respectively in color comparison tube, add borate buffer solution, sodium bicarbonate, the H containing cetyl ammonium bromide (CTAB)
2o
2rock mixing with potassium rhodanide, after mixing, add gold nanorods again, shake up; Then at 50-100 DEG C of temperature, hatch 2-30 minute, carry out chromogenic reaction, use professional camera record colour developing result, after gathering picture, form cobalt standard color comparison card F with the picture by color gradient.
8. by the method for the detection cobalt ions of the cobalt ions colorimetric determination kit described in claim 5 or 6, it is characterized in that: described containing cetyl ammonium bromide (CTAB) borate buffer solution, pH is 7.4-9.6, and concentration is 10-200mM; Described sodium bicarbonate solution concentration is 0.01-1M; Described H
2o
2solution concentration is 0.01-1M; Described potassium thiocyanate solution concentration is 0.01-1M.
9. by the method for the detection cobalt ions of cobalt ions colorimetric determination kit according to claim 7, it is characterized in that: described is 9.0 containing cetyl ammonium bromide (CTAB) borate buffer solution pH, and concentration is 50mM, and the concentration containing CTAB is 2mM; The length-diameter ratio of gold nanorods solution to be concentration be 2.3nM is the gold nanorods solution of 2:1; Sodium bicarbonate solution concentration is 0.1M; H
2o
2solution concentration is 0.1M; Potassium thiocyanate solution concentration is 0.1M.
10. by the method for the detection cobalt ions of the cobalt ions colorimetric determination kit described in claim 5 or 6, it is characterized in that: gold nanorods solution is turned the/centrifugal 5-30min of min at 6000-10000, collecting precipitation also dilutes with intermediate water, / centrifugal the 5-30min of min is turned with 5000-9000 again after dilution, precipitation is again with intermediate water dilution, stand-by.
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|---|---|---|---|
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|---|---|---|---|
| CN201310179630.5A CN103411962B (en) | 2013-05-15 | 2013-05-15 | A kind of cobalt ions colorimetric determination kit and detection method thereof |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108827952A (en) * | 2018-07-02 | 2018-11-16 | 北京师范大学 | NOR logic gate and its construction method |
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| CN103954618A (en) * | 2014-04-23 | 2014-07-30 | 叶伟荣 | Method for determining concentration of glucose by using colorimetric method |
| CN106290195A (en) * | 2016-08-30 | 2017-01-04 | 中南林业科技大学 | Gold contracted payment nanometer colorimetric sensor preparation method and the method for detection cobalt ion thereof |
| CN106568773B (en) * | 2016-11-22 | 2020-02-21 | 中国科学院烟台海岸带研究所 | Manganese ion colorimetric detection kit and detection method thereof |
| CN106770208A (en) * | 2016-12-12 | 2017-05-31 | 济南大学 | A kind of method that ferrous ion is detected based on gold nanorods |
| CN109030476B (en) * | 2018-07-20 | 2020-09-01 | 安徽原上草节能环保科技有限公司 | Preparation method of detection paper tube for cobalt ions, detection paper tube and detection method |
| CN113984751B (en) * | 2021-10-29 | 2024-05-03 | 中南林业科技大学 | Gold nano star colorimetric sensor, preparation method thereof and Co detection method2+Is a method of (2) |
| CN115015230B (en) * | 2022-02-22 | 2024-03-01 | 中国科学院生态环境研究中心 | Rapid detection method and device for hexabromocyclododecane content in EPS/XPS |
| CN117554362B (en) * | 2024-01-10 | 2024-03-12 | 中国科学院烟台海岸带研究所 | Method for detecting cyanide at normal temperature with high sensitivity and nano colorimetric analysis kit |
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