CN103409486A - Method for improving adenosine fermentation output through feeding hypoxanthine - Google Patents

Abstract

The invention provides a method for improving the adenosine fermentation output through feeding hypoxanthine. The method which treats Bacillus subtilis XGL as a producing strain is characterized in that a precursor is intermittently fed at a uniform speed 6-12 times after the strains grow in a logarithmic phase. The utilization of the method to carry out the adenosine fermentation production makes the precursor in a metabolism flow for the adenosine synthesis sufficient, so the adenosine output is improved to 33.5g/L, and is 28.8% higher than the output obtained through other methods, and the fermentation period is shortened to 50h. The method has the advantages of adenosine output increase, and simple and repeatable operation.

Description

A kind of method that adds xanthoglobulin raising adenosine fermentation output by stream

Technical field

The invention belongs to microorganism and fermentation engineering field, be specifically related to a kind of method that the precursor fermentation production of adenosine is added in centre.

Background technology

Adenosine (Adenosine), be adenosine, and molecular formula is C ₁₀H ₁₃N ₅O ₄, molecular weight is 267.24, chemical name is 9-β-D-furans RiboseBase VITAMIN B4 (9-β-D-ribofuranosyl adenine).Adenosine, as the intermediate product of energy metabolism of myocardial, is being brought into play important pathophysiological role in the homeostasis that maintains cardiovascular systems and various damage mechanism.Adenosine is the first-line drug of the treatment paroxysmal supraventricular tachycardia (Paroxysmal supraventricular tachycardia, PSVT) of FDA approval in the U.S., and becomes the routine administration of emergency Treatment tachyarrhythmia and drug load test.

Continuous expansion along with the gland glycoside action scope, its market requirement is also in continuous growth, and the production method of adenosine mainly contains chemical synthesis, enzyme process, fermentation method, and the production of current domestic adenosine is mainly chemical method and enzyme process, cost is high, seriously polluted, applies and is subject to serious restriction.Producing adenosine through zymotechnics have a reaction conditions gentleness, cost is low, the advantages such as green, clean, environmental protection.Therefore, under the situation of low-carbon environment-friendly, use producing adenosine through zymotechnics, optimize the zymotechnique of adenosine, further improve the output of adenosine, have great importance.

Summary of the invention

The present invention seeks to solve existing chemical method and Production by Enzymes adenosine cost high, seriously polluted, apply limitedly, and the lower problem of producing adenosine through zymotechnics output provides a kind of and adds by stream the method that precursor improves adenosine fermentation output.The method is added hypoxanthic fed-batch fermentation in the middle of adopting, can for bacterial metabolism, provide the precursor substance of shortage in the middle and later periods of fermentation, removes the bottleneck of precursor supply, is conducive to the efficient synthetic of metabolism target product.

The concrete technical scheme of the present invention

A kind of method that adds precursor raising adenosine fermentation output by stream, the method adopts intermittently the mode batch fermentation of at the uniform velocity feed supplement to produce adenosine, fermentation strain employing subtilis ( Bacillus subtilis) XGL (publish at Chinese biological engineering magazine on December 31st, 2011, and article is " to cross and express by this bacterial strain PurAThe impact of gene pairs adenosine accumulation ", now be preserved in DSMZ of biotechnology institute of University Of Science and Technology Of Tianjin).

The concrete operations of the inventive method comprise following process:

(1) slant culture: by subtilis ( Bacillus subtilis) XGL is seeded to activated inclined plane, cultivates 20 ~ 28 h for 28 ~ 32 ℃;

(2) seed culture: by transfering loop scraping slant strains, be seeded to seed culture medium, 28 ~ 33 ℃, 180 ~ 220 rpm cultivate 8 ~ 11 h, as fermentation kind of liquid;

(3) batch fermentation is cultivated: the inoculum size by 10 ~ 15% is forwarded in the fermentor tank that fermention medium is housed, and 30 ~ 34 ℃, dissolved oxygen maintains 10 ~ 20%, uses NH ₃H ₂O regulates pH, and pH is 6.0 ~ 7.0, incubation time 50 ~ 60 h, and at thalline OD _600*20Reach after 1.0 ~ 1.5, for example 18 ~ 30 h start, every 3 ~ 6 h intermittently at the uniform velocity stream add xanthoglobulin solution, concentration maintains 1 ~ 5g/L, stream adds 6 ~ 12 times altogether, each 20 ~ 50 mL.

Wherein, described inclined-plane solid medium comprises following component in g/L: glucose 1 ~ 3, and peptone 5 ~ 10, extractum carnis 5 ~ 10, yeast powder 2 ~ 5, NaCl 1 ~ 2.5, agar 20 ~ 30, pH 7.0 ~ 7.2.

Described seed culture medium, comprise following component in g/L: oral glucose 10 ~ 20, corn steep liquor 15 ~ 25 mL/L, soybean meal hydrolysate 5 ~ 10mL/L, yeast powder 5 ~ 10, monosodium glutamate 3 ~ 8, peptone 5 ~ 10, KH ₂PO ₄0.5 ~ 1.5, MgSO ₄7H ₂O 0.2 ~ 0.8, urea 2 ~ 7, and xanthine 0.01 ~ 0.05, Histidine 0.01 ~ 0.05, pH 7.0 ~ 7.2.

Described liquid fermentation medium, comprise following component in g/L: oral glucose 80 ~ 120, yeast extract paste 12 ~ 18, monosodium glutamate 8 ~ 12, K ₂HPO ₄1 ~ 3, xanthine 0.01 ~ 0.05, Histidine 0.01 ~ 0.05, MgSO ₄7H ₂O 1 ~ 5, and MnSO4 0.003 ~ 0.006, FeSO ₄7H ₂O 0.003 ~ 0.006, CaCl ₂1 ~ 3, corn steep liquor 10 ~ 20 mL/L, soybean meal hydrolysate 10 ~ 30 mL/L.

In sum, the present invention be take subtilis XGL and is fermented bacterium, and fermention medium is optimized, at the enterprising interline in the basis of 5L fermentor tank lab scale, add the fermentation control of precursor, remove the bottleneck of precursor supply, extend the production time of meta-bolites, improve the output of adenosine.

 

Advantage of the present invention and beneficial effect:

(1) with traditional chemical method, compare with enzyme process, production unit is simple, and Green-pollution is little, meets current low-carbon (LC) environment, and cost is low, is applicable to scale operation.

(2) microorganism strains of the present invention's use, the stable loss that is difficult for of genetic marker, pass switching more than ten generations, the output kept stable.

(3) the present invention adds the precursor xanthoglobulin between during the fermentation, and the production intensity of adenosine is improved, and reaches 33.5 g/L, compares with non-adding, and output improves 28.8%.

 

Embodiment

Referring to specific embodiment, the present invention is described.Those skilled in the art will appreciate that the described concrete material ratio of these specific exampless, processing condition and result thereof are only for the present invention, the scope that it does not limit the present invention in any way.

Below the tunning adenosine content adopts in each embodiment analytical procedure as follows:

In fermented liquid, the product adenosine adopts instrument Agilent 1100 high performance liquid chromatographs to measure (HPLC).Chromatographic column is Kromasil C18 post (250 mm * 416 mm i.d., 5 μ m), and 30 ℃ of column temperatures, detector are UV-detector (259nm), and moving phase is water: acetonitrile=90:10; Flow velocity 1.0 ml/min; Sample size 20 μ L.Accurately take the standard substance of adenosine, be configured to deionized water the standard solution that mass concentration is 1 g/L; Pretreated fermentation broth sample to be measured is diluted to suitable concentration with deionized water, and with 0.22 μ m filtering with microporous membrane, to be detected as fermented sample solution, fermented sample is according to calculated by peak area adenosine output.

 

Embodiment 1:

The slant culture based formulas is in g/L: glucose 2, and peptone 10, extractum carnis 10, yeast powder 5, NaCl 2.5, agar 30, pH 7.0.

The seed culture based formulas is in g/L: oral glucose 20, corn steep liquor 25 mL/L, soybean meal hydrolysate 10 mL/L, yeast powder 5, monosodium glutamate 5, peptone 8, KH ₂PO ₄1, MgSO ₄7H ₂O 0.4, urea 5, and xanthine 0.03, Histidine 0.03, pH 7.2.

Fermention medium is in g/L: oral glucose 100, yeast extract paste 18, monosodium glutamate 12, K ₂HPO ₄3, xanthine 0.03, L-Histidine 0.03, MgSO ₄7H ₂O 5, MnSO ₄0.006, FeSO ₄7H ₂O 0.006, CaCl ₂2, corn steep liquor 15 mL/L, soybean meal hydrolysate 10 mL/L.

The subtilis XGL of take is starting strain, is seeded to activated inclined plane, after 30 ℃ of cultivation 22 h, is seeded to seed culture medium by transfering loop scraping slant strains, and 31 ℃, 200 rpm cultivate 10.5 h; By 10%(v/v) inoculum size be forwarded in the 5 L fermentor tanks that fermention medium is housed, 32 ℃, dissolved oxygen maintains 10 ~ 20%, uses NH ₃H ₂O regulates pH, and pH maintains 6.5, glucose residual quantity in the on-line monitoring fermented liquid, as its most 3g/L that descends, stream adds glucose to fermented liquid, and to maintain the residual sugar amount be 3g/L, and at 20 h, 24 h, 28 h, 32 h, 36 h, 40 h, at the uniform velocity stream adds the xanthoglobulin solution that concentration is 2.5 g/L respectively, every 4 hours stream, add once, stream adds 6 times, each 50 mL, and the stream dosage is in the 300mL left and right, 50 h that ferment, adenosine output reaches 33.5 g/L.

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Embodiment 2:

The slant culture based formulas is in g/L: glucose 1, and peptone 5, extractum carnis 5, yeast powder 2, NaCl 1, agar 20, pH 7.0.

The seed culture based formulas is in g/L: oral glucose 10, corn steep liquor 15 mL/L, soybean meal hydrolysate 5 mL/L, yeast powder 5, monosodium glutamate 3, peptone 5, KH ₂PO ₄0.5, MgSO ₄7H ₂O 0.2, urea 2, and xanthine 0.01, Histidine 0.01, pH 7.2.

Fermention medium is in g/L: oral glucose 80, yeast extract paste 12, monosodium glutamate 8, K ₂HPO ₄1, xanthine 0.01, L-Histidine 0.01, MgSO ₄7H ₂O 1, MnSO ₄0.003, FeSO ₄7H ₂O 0.003, CaCl ₂1, corn steep liquor 10 mL/L, soybean meal hydrolysate 10 mL/L.

The subtilis XGL of take is starting strain, is seeded to activated inclined plane, after 28 ℃ of cultivation 20 h, is seeded to seed culture medium by transfering loop scraping slant strains, and 28 ℃, 180 rpm cultivate 8 h; By 10%(v/v) inoculum size be forwarded in the 5 L fermentor tanks that fermention medium is housed, 33 ℃, dissolved oxygen maintains 10 ~ 20%, uses NH ₃H ₂O regulates pH, pH maintains 6.0, glucose residual quantity in the on-line monitoring fermented liquid, as its most 3g/L that descends, stream adds glucose to fermented liquid, and to maintain the residual sugar amount be 3g/L, to add concentration be 1g/L xanthoglobulin solution to every 3 h streams between 20 ~ 50 h, stream adds 12 times altogether, each 20mL, and the stream dosage is in the 240mL left and right, 60 h that ferment, adenosine output reaches 31.8 g/L.

 

Embodiment 3:

The slant culture based formulas is in g/L: glucose 3, and peptone 10, extractum carnis 10, yeast powder 5, NaCl 2.5, agar 30, pH 7.2.

The seed culture based formulas is in g/L: oral glucose 20, corn steep liquor 25 mL/L, soybean meal hydrolysate 10 mL/L, yeast powder 10, monosodium glutamate 8, peptone 10, KH ₂PO ₄1.5, MgSO ₄7H ₂O 0.8, urea 7, and xanthine 0.05, Histidine 0.05, pH 7.2.

Fermention medium is in g/L: oral glucose 120, yeast extract paste 18, monosodium glutamate 12, K ₂HPO ₄3, xanthine 0.05, L-Histidine 0.05, MgSO ₄7H ₂O 5, MnSO ₄0.006, FeSO ₄7H ₂O 0.006, CaCl ₂3, corn steep liquor 20 mL/L, soybean meal hydrolysate 30 mL/L.

The subtilis XGL of take is starting strain, is seeded to activated inclined plane, after 32 ℃ of cultivation 24 h, is seeded to seed culture medium by transfering loop scraping slant strains, and 33 ℃, 220 rpm cultivate 11 h; By 10%(v/v) inoculum size be forwarded in the 5 L fermentor tanks that fermention medium is housed, 34 ℃, dissolved oxygen maintains 10 ~ 20%, uses NH ₃H ₂O regulates pH, pH maintains 7.0, glucose residual quantity in the on-line monitoring fermented liquid, as its most 3g/L that descends, stream adds glucose to fermented liquid, and to maintain the residual sugar amount be 3g/L, to add concentration be 5g/L xanthoglobulin solution to every 6 h streams between 20 ~ 50 h, stream adds 8 times altogether, each 25mL, and the stream dosage is in the 200mL left and right, 60 h that ferment, adenosine output reaches 30.6 g/L.

 

Comparative Examples 1:

In the middle of not adopting, add the method for xanthoglobulin (additionally not adding xanthoglobulin in fermention medium) and carry out fermentation culture, other conditions are all identical with the method for embodiment 1.Final detection adenosine content is 26 g/L.

 

Comparative Examples 2:

At 20 h, 35 h, 50 h, at the uniform velocity stream adds 2.5 g/L xanthoglobulin solution respectively, adds once every 15 h streams, and stream adds 3 times, each 50 mL, and flow 150 mL left and right, other conditions are all identical with the method for embodiment 1.Final detection adenosine content is 28.9 g/L.

By embodiment 1 and Comparative Examples 1, can be found out, in fermenting process, stream adds the output that xanthoglobulin solution can significantly improve adenosine, adenosine output improves 28.8%, and this shows that hypoxanthic interpolation has made up the deficiency of precursor in the fermenting process, removes the bottleneck of precursor supply.By Comparative Examples 2, can find out, the interpolation number of times of xanthoglobulin solution and the difference of additional time, accumulation for adenosine has larger impact, repeatedly, short interval can better maintain the ability that subtilis XGL produces adenosine, makes the output of adenosine reach higher level.

Claims (2)

1. one kind adds by stream the method that xanthoglobulin improves adenosine fermentation output, and described method is intermittently at the uniform velocity added the precursor fermentation production of adenosine in the middle of adopting process, fermentation strain be subtilis ( Bacillus subtilis) XGL, specifically comprise following process:

(1) slant culture: the subtilis XGL of take is starting strain, is seeded to activated inclined plane, cultivates 20 ~ 28 h for 28 ~ 32 ℃;

(2) seed culture: by transfering loop scraping slant strains, be seeded to seed culture medium, 28 ~ 33 ℃, 180 ~ 220 rpm cultivate 8 ~ 11 h, as fermentation seed liquid;

(3) batch fermentation is cultivated: the inoculum size by 10 ~ 15% is forwarded in the fermentor tank that fermention medium is housed, and 30 ~ 34 ℃, dissolved oxygen maintains 10 ~ 20%, uses NH ₃H ₂O regulates pH and is controlled at 6.0 ~ 7.0, and incubation time 50-60 h, at thalline OD _600*20Reach after 1.0 ~ 1.5, every 3 ~ 6 h intermittently at the uniform velocity stream add the precursor xanthoglobulin, interpolations concentration is 1 ~ 5g/L, common stream adds 6 ~ 12 times, each 20 ~ 50mL.

2. according to the described method of claims 1, it is characterized in that:

(1) the slant culture based component is in g/L: glucose 1 ~ 3, and peptone 5 ~ 10, extractum carnis 5 ~ 10, yeast powder 2 ~ 5, NaCl 1 ~ 2.5, agar 20 ~ 30, pH 7.0 ~ 7.2;

(2) the seed culture based component is in g/L: oral glucose 10 ~ 20, corn steep liquor 15 ~ 25 mL/L, soybean meal hydrolysate 5 ~ 10mL/L, yeast powder 5 ~ 10, monosodium glutamate 3 ~ 8, peptone 5 ~ 10, KH ₂PO ₄0.5 ~ 1.5, MgSO ₄7H ₂O 0.2 ~ 0.8, urea 2 ~ 7, and xanthine 0.01 ~ 0.05, Histidine 0.01 ~ 0.05, pH 7.0 ~ 7.2;

(3) the liquid fermentation medium composition is in g/L: oral glucose 80 ~ 120, yeast extract paste 12 ~ 18, monosodium glutamate 8 ~ 12, K ₂HPO ₄1 ~ 3, xanthine 0.01 ~ 0.05, Histidine 0.01 ~ 0.05, MgSO ₄7H2O 1 ~ 5, MnSO ₄0.003 ~ 0.006, FeSO ₄7H ₂O 0.003 ~ 0.006, CaCl ₂1 ~ 3, corn steep liquor 10 ~ 20 mL/L, soybean meal hydrolysate 10 ~ 30 mL/L.