CN103386343A - Liquid nitrogen water-bath device - Google Patents
Liquid nitrogen water-bath device Download PDFInfo
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- CN103386343A CN103386343A CN2013102640559A CN201310264055A CN103386343A CN 103386343 A CN103386343 A CN 103386343A CN 2013102640559 A CN2013102640559 A CN 2013102640559A CN 201310264055 A CN201310264055 A CN 201310264055A CN 103386343 A CN103386343 A CN 103386343A
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- liquid nitrogen
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Abstract
The invention discloses a liquid nitrogen water-bath device which solves problems that an extract or a culture can be easily spilled, cooling of the extract or culture in an EP tube is not uniform and working efficiency is low during a molecular or a microbiological experiment or when the extract or the culture is subjected to an EP-tube ice bath with a prior art. The water-bath device comprises a first tank, a liquid nitrogen injection tube, a second tank, a trough body and an EP pipe frame, wherein the second tank comprises a baseplate and multiple vertical plates; the first plate has a tank cap on the top; the second tank comprises multiple vertical plates and the baseplate; the trough body is set inside the second tank; the inner wall of the second tank and the outer wall of the trough body are sealed to form a closed cavity; the EP pipe frame is set in the trough body; one end of the liquid nitrogen injection tube is set on the second tank after passing through the side wall of the first tank; and the liquid nitrogen injection tube is communicated to the closed cavity. The liquid nitrogen water-bath device is applicable to molecular biological and microbiological experiments.
Description
Technical field
The present invention relates to a kind of liquid nitrogen bain-marie.
Background technology
At present, when doing molecule or Experiment on Microbiology, usually take the method for ice bath for protection extract or culture, be about to extract and put in EP pipe (the Eppendorf pipe, also be centrifuge tube), then the EP pipe is placed in freezing after breaking into pieces.This conventional method is difficult for fixedly EP manages and material is spilt, and, due to the gap that exists between ice cube, causes the material in the EP pipe can not evenly catch a cold and be protected to greatest extent inefficiency.
Summary of the invention
The present invention is in order to solve in prior art when doing molecule or Experiment on Microbiology, when extract or culture are managed ice bath by EP, extract or culture easily spill and EP pipe in extract or culture inhomogeneous, the ineffective problem of catching a cold, and then provide a kind of liquid nitrogen bain-marie.
The present invention addresses the above problem the technical scheme that adopts to be: described bain-marie comprises the first casing, liquid nitrogen ascending pipe, the second casing, cell body and EP pipe support, and the second casing comprises base plate and a plurality of riser; The first casing is the casing of top with case lid, the second casing that the second casing is comprised of a plurality of risers and base plate, cell body is arranged in the second casing, the madial wall of the second casing and the sealing of the lateral wall of cell body form the cavity of a sealing, the EP pipe support is arranged in cell body, the sidewall that one end of liquid nitrogen ascending pipe passes the first casing is arranged on the second casing, and the liquid nitrogen ascending pipe is communicated with the cavity of sealing.
The invention has the beneficial effects as follows: 1, by the present invention, the EP pipe can be placed on EP pipe pipe support, prevented that the interior extract of EP pipe or culture from spilling, 2, by injecting liquid nitrogen in the cavity that seals in the present invention, put into cold water in cell body extract or the culture of EP pipe are caught a cold evenly, keep the required low temperature environment of experiment.Operating efficiency when 3, the present invention manages ice bath than EP in prior art has improved 15%-30%, and the present invention has simple in structure, characteristics easy to use.
Description of drawings
Fig. 1 is overall structure schematic diagram of the present invention.
The specific embodiment
The specific embodiment one: in conjunction with Fig. 1, a kind of liquid nitrogen bain-marie of present embodiment is described, described bain-marie comprises that the first casing 1, liquid nitrogen ascending pipe 2, the second casing 4, cell body 6 and EP pipe support 5, the second casings 4 comprise base plate 9 and a plurality of riser 8; The first casing 1 is the casing of top with case lid, the second casing 4 that the second casing 4 is comprised of a plurality of risers 8 and base plate 9, cell body 6 is arranged in the second casing 4, the lateral wall sealing of the madial wall of the second casing 4 and cell body 6 forms the cavity 7 of a sealing, EP pipe support 5 is arranged in cell body 6, the sidewall that one end of liquid nitrogen ascending pipe 2 passes the first casing 1 is arranged on the second casing 4, and liquid nitrogen ascending pipe 2 is communicated with the cavity 7 of sealing.
The specific embodiment two: a kind of liquid nitrogen bain-marie of present embodiment is described in conjunction with Fig. 1, described bain-marie also includes pipe close 3, enter on the other end that pipe close 3 is arranged on liquid nitrogen ascending pipe 2, enter pipe close 3 and can prevent the liquid nitrogen volatilization, ascending pipe 2 is convenient to the injection of liquid nitrogen, and other is identical with the specific embodiment one.
The specific embodiment three: a kind of liquid nitrogen bain-marie of present embodiment is described in conjunction with Fig. 1, described the first casing 1 comprises many foldings 10, the side plate of the first casing 1 connects by folding 10 with case lid, if folding 10 is set to be convenient to need to be interrupted while preparing other experiments in the experimental implementation process, can cover the low temperature that case lid keeps environment in case, other is identical with the specific embodiment one.
The specific embodiment four: a kind of liquid nitrogen bain-marie of present embodiment is described in conjunction with Fig. 1, described bain-marie also comprises handle 11, handle 11 is arranged on the case lid of the first casing 1, handle 11 the switch that can facilitate case lid is set, other is identical with the specific embodiment three.
The specific embodiment five: in conjunction with Fig. 1, a kind of liquid nitrogen bain-marie of present embodiment is described, the number of described riser 8 is four to six, and other is identical with the specific embodiment one.
The specific embodiment six: a kind of liquid nitrogen bain-marie of present embodiment is described in conjunction with Fig. 1, described the first casing 1 is the first casing 1 of being made by polystyrene foam plate, polystyrene foam plate be have low, the high resistance to compression of thermal conductivity, protection against the tide, airtight, do not absorb water, the useful performance such as light weight, corrosion-resistant, long service life, also to avoid testing the situation that behaviour person damages, other is identical with the specific embodiment one.
The specific embodiment seven: a kind of liquid nitrogen bain-marie of present embodiment is described in conjunction with Fig. 1, the cavity 7 of described sealing is the cavity 7 of the sealing of being made by stainless steel material, it is good that stainless steel material has cryogenic property, the characteristics that intensity is high, and other is identical with the specific embodiment one.
Operation principle
During work, liquid nitrogen is injected liquid nitrogen in the cavity 7 of sealing by liquid nitrogen ascending pipe 2, will enter pipe close 3 and be placed on liquid nitrogen ascending pipe 2, the EP pipe is placed on EP pipe support 5, reach the purpose that keeps the even low temperature of experimental situation by liquid nitrogen refrigerating.
Claims (7)
1. liquid nitrogen bain-marie, it is characterized in that: described bain-marie comprises the first casing (1), liquid nitrogen ascending pipe (2), the second casing (4), cell body (6) and EP pipe support (5), and the second casing (4) comprises base plate (9) and a plurality of riser (8); The first casing (1) is the casing of top with case lid, the second casing (4) that the second casing (4) is comprised of a plurality of risers (8) and base plate (9), cell body (6) is arranged in the second casing (4), the lateral wall sealing of the madial wall of the second casing (4) and cell body (6) forms the cavity (7) of a sealing, EP pipe support (5) is arranged in cell body (6), the sidewall that one end of liquid nitrogen ascending pipe (2) passes the first casing (1) is arranged on the second casing (4), and liquid nitrogen ascending pipe (2) is communicated with the cavity (7) of sealing.
2. a kind of liquid nitrogen bain-marie according to claim 1, it is characterized in that: described bain-marie also includes pipe close (3), enters pipe close (3) and is arranged on the other end of liquid nitrogen ascending pipe (2).
3. a kind of liquid nitrogen bain-marie according to claim 1, it is characterized in that: described the first casing (1) comprises many foldings (10), the side plate of the first casing (1) connects by folding (10) with case lid.
4. a kind of liquid nitrogen bain-marie according to claim 3, it is characterized in that: described bain-marie also comprises handle (11), handle (11) is arranged on the case lid of the first casing (1).
5. a kind of liquid nitrogen bain-marie according to claim 1, it is characterized in that: the number of described riser (8) is four to six.
6. a kind of liquid nitrogen bain-marie according to claim 1, it is characterized in that: described the first casing (1) is the first casing (1) of being made by polystyrene foam plate.
7. a kind of liquid nitrogen bain-marie according to claim 1 is characterized in that: the cavity of described sealing (7) is the cavity (7) of the sealing of being made by stainless steel material.
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CN2013102640559A CN103386343A (en) | 2013-06-28 | 2013-06-28 | Liquid nitrogen water-bath device |
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CN2013102640559A CN103386343A (en) | 2013-06-28 | 2013-06-28 | Liquid nitrogen water-bath device |
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CN2013102640559A Pending CN103386343A (en) | 2013-06-28 | 2013-06-28 | Liquid nitrogen water-bath device |
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Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN103884531A (en) * | 2014-04-11 | 2014-06-25 | 中国商用飞机有限责任公司 | Cooling test method for achieving low-temperature environment and cooling device used in low-temperature environment |
Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN2550699Y (en) * | 2002-02-19 | 2003-05-14 | 王德和 | Improvement of condensing plant |
TWM394447U (en) * | 2010-07-29 | 2010-12-11 | Nat Museum Of Marine Biology & Aquarium | Freezing storage container device |
-
2013
- 2013-06-28 CN CN2013102640559A patent/CN103386343A/en active Pending
Patent Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN2550699Y (en) * | 2002-02-19 | 2003-05-14 | 王德和 | Improvement of condensing plant |
TWM394447U (en) * | 2010-07-29 | 2010-12-11 | Nat Museum Of Marine Biology & Aquarium | Freezing storage container device |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN103884531A (en) * | 2014-04-11 | 2014-06-25 | 中国商用飞机有限责任公司 | Cooling test method for achieving low-temperature environment and cooling device used in low-temperature environment |
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Application publication date: 20131113 |