CN103385767A - Mouse gall collecting device and method for operating mouse gall collecting device on mouse model - Google Patents
Mouse gall collecting device and method for operating mouse gall collecting device on mouse model Download PDFInfo
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- CN103385767A CN103385767A CN2013103401593A CN201310340159A CN103385767A CN 103385767 A CN103385767 A CN 103385767A CN 2013103401593 A CN2013103401593 A CN 2013103401593A CN 201310340159 A CN201310340159 A CN 201310340159A CN 103385767 A CN103385767 A CN 103385767A
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Abstract
The invention provides a mouse gall collecting device, comprising a fixed support, wherein the fixed support is connected with at least one gall collecting unit, each gall collecting unit comprises a mouse rearing cage and a gall collecting device, each gall collecting device comprises a liquid storage cup for storing gall, and a gall bladder cannula with one end being connected with the corresponding liquid storage cup and the other end being implanted into the gall bladder of a mouse, one end, positioned at the gall bladder of the mouse, of each gall bladder cannula is provided with an expansion end, each gall bladder cannula is connected with a vertically drooped positioning iron wire, one end of each positioning iron wire is movably connected with the fixed support, and the other end of each positioning iron wire is fixedly arranged at the neck of the mouse. The invention has the beneficial effects that the mouse gall collecting device has the advantages that long-term mouse gall collecting can be completed under the condition of not influencing the normal activity of the mouse, the structure is simple, the operation is convenient, safety and reliability are realized, and the gall of the mouse can be collected for a long time; and a method for operating the mouse gall collecting device on a mouse model enables a user of the device to obtain sufficient use mastery before a live test is carried out, so that the success probability of an operation is improved.
Description
Technical field
The present invention relates to the animal body fluid gathering-device, especially mice bile gathering-device, specifically, be a kind of device by gallbladder intubate collection mice bile and the operational approach on mouse model thereof.
Background technology
It is in giving medicine or nutrient that animal gallbladder intubate is collected the bile model, studies its absorption through liver, decomposition, metabolism model more accurately, in the animal experiment studies such as toxicology, pharmacology and threpsology, critical role is arranged.Thereby the liver metabolism study general obtains experimental data with the model structure that the relatively large animal via cystic duct cannula such as Canis familiaris L., rabbit and rat is collected bile at present, but only with relatively large animal, as experimental subject, can make experimental result have one-sidedness and inaccuracy; And when choosing meiofauna as experimental subject, tend to select mice, but the mice common bile duct is tiny, and the intubation procedure of existing experimental model very easily damages the common bile duct of mice, is unfavorable for long-term observation and in the situation that the free movable bile of collecting of mice., if can invent a kind ofly can not affect the normal work and rest of mice and can continue to collect again the gathering-device of mice bile, can effectively collect enough mice bile and be used for experiment.Simultaneously, comparatively complicated during due to this device practical operation, need to carry out the operation of more time on mouse model before the live body operation.
Summary of the invention
Technical problem to be solved by this invention is for above-mentioned prior art present situation, and a kind of mice bile gathering-device and the operational approach on mouse model thereof are provided, and this mice bile gathering-device can be implanted the interior bile of collection activity Mice Body in the mice gallbladder.It is convenient that this apparatus structure is simple, bile is collected, and can collect for a long time mice bile.Disclosed by the invention with this application of installation the operational approach on mouse model, have the effect that can effectively promote the proficiency of user.
The present invention solves the problems of the technologies described above the technical scheme that adopts:
a kind of mice bile gathering-device, include fixed support, wherein, fixed support is connected with at least one bile collector unit, each bile collector unit includes a mice rearging cage and a bile gathering-device, liquid storing cup and an end that the bile gathering-device includes for storage bile are connected with liquid storing cup, the other end is implanted the gallbladder intubate of mice gallbladder, the end that the gallbladder intubate is positioned at the mice gallbladder expands to be formed with and expands end, be connected with a vertical sagging location iron wire on the gallbladder intubate, location iron wire one end and fixed support are flexibly connected, the other end is fixed on the cervical region of mice.
Above-mentioned liquid storing cup is fixedly connected with the location iron wire.
Above-mentioned fixed support consists of the montant that a suspension rod and interval arrange, each bile collector unit all with montant secure fit accordingly.
Be arranged at intervals with rotating shaft on above-mentioned suspension rod, rotating shaft connects the location iron wire.
Above-mentioned gallbladder intubate is crooked sagging and be placed in liquid storing cup in the lower end of rotating shaft.
Be connected and fixed by lashing wire between above-mentioned liquid storing cup and described location iron wire.
Be provided with the drinking-water cylinder in above-mentioned mice rearging cage, the lower end of drinking-water cylinder is provided with drinking tube.
A kind of operational approach of using mice bile gathering-device on mouse model comprises the following steps:
Step 1, preparation mouse model, be provided with the gallbladder model in proportion in the mouse model abdomen;
Step 2, prepare the gallbladder intubate, straight type polyurethane conduit is elongated make long S type structure in the water of 80 ℃, then the polyurethane catheter of S type one end is melted on the electric cautery of 100 ℃ formation and expand end, make the gallbladder intubate;
The length of above-mentioned straight type polyurethane conduit is 15cm, internal diameter≤1mm.
Adopt suture that a fast knot is fixed in gallbladder model middle and lower part in above-mentioned step 3, and suture is No. 6 sutures.
compared with prior art, a kind of mice bile gathering-device of the present invention, include fixed support, wherein, fixed support is connected with at least one bile collector unit, each bile collector unit includes a mice rearging cage and a bile gathering-device, liquid storing cup and an end that the bile gathering-device includes for storage bile are connected with liquid storing cup, the other end is implanted the gallbladder intubate of mice gallbladder, the end that the gallbladder intubate is positioned at the mice gallbladder expands to be formed with and expands end, the gallbladder intubate is connected with the location iron wire that is fixed on the mouse carotid back, rotating shaft on location iron wire one end and fixed support is flexibly connected.This device can be implanted the mice gallbladder with the gallbladder intubate, and mice is put into the mice rearging cage let alone free activity,, in the situation that do not affect the mice activity, complete the collection of mice bile, can be used for the collection of long-term mice bile, can also carry out bile to many mices simultaneously and collect.Simple in structure with the mouse model that this device is complementary, can allow the user of this device just can obtain enough use proficiencys before live test, improve the probability of successful surgery.
Description of drawings
Fig. 1 is the structural representation of the embodiment of the present invention;
Fig. 2 is the structural representation of bile collector unit in Fig. 1;
Fig. 3 is I section enlarged drawing in Fig. 2.
The specific embodiment
Embodiment is described in further detail the present invention below in conjunction with accompanying drawing.
Be structural representation of the present invention as shown in Figure 1 to Figure 3,
Reference numeral wherein is: fixed support 1, suspension rod 11, montant 12, rotating shaft 13, connection tube 14, mice rearging cage 2, location iron wire 3, gallbladder intubate 4, expand end 41, liquid storing cup 5, lashing wire 6, drinking-water cylinder 7, drinking tube 71.
As shown in Figure 1 to Figure 3,
a kind of mice bile gathering-device, include fixed support 1, wherein, fixed support 1 is connected with several bile collector units, each bile collector unit includes a mice rearging cage 2 and a bile gathering-device, liquid storing cup 5 and an end that the bile gathering-device includes for storage bile are connected with liquid storing cup 5, the other end is implanted the gallbladder intubate 4 of mice gallbladder, the end that gallbladder intubate 4 is positioned at the mice gallbladder expands to be formed with and expands end 41, be connected with a vertical sagging location iron wire 3 on gallbladder intubate 4, location iron wire 3 one ends and fixed support 1 are flexibly connected, the other end is fixed on the cervical region of mice.
In embodiment, liquid storing cup 5 is fixedly connected with location iron wire 3.
In embodiment, fixed support 1 consists of the montant 12 that a suspension rod 11 and interval arrange, each bile collector unit all with montant 12 secure fit accordingly.The bile collector unit is arranged side by side, compartment of terrain is arranged on described fixed support 1; A plurality of bile collector units are set up in parallel, and can carry out simultaneously the collection of bile to a plurality of mices,, to increase the collection of experimental data, increase the comparative between experimental data, thereby improve the accuracy of experiment.
In embodiment, be arranged at intervals with rotating shaft 13 on suspension rod 11, the lower end of rotating shaft 13 is provided with connection tube 14, and location iron wire 3 passes this connection tube 14 and is connected cooperation with it with the end that fixed support 1 is flexibly connected.
In embodiment, gallbladder intubate 4 is crooked sagging and be placed in liquid storing cup 5 in the lower end of rotating shaft 13.
In embodiment, be connected and fixed by lashing wire 6 between liquid storing cup 5 and described location iron wire 3.
In embodiment, be provided with drinking-water cylinder 7 in mice rearging cage 2, the lower end of drinking-water cylinder 7 is provided with drinking tube 71.
Comparatively complicated during device practical operation of the present invention, should carry out the operation of more time on mouse model before the live body operation, therefore, the invention discloses a kind of operational approach of using mice bile gathering-device on mouse model, comprise the following steps:
Step 1, preparation mouse model, be provided with the gallbladder model in proportion in the mouse model abdomen;
Step 2, preparation gallbladder intubate 4, elongate straight type polyurethane conduit and make long S type structure in the water of 80 ℃, then the polyurethane catheter of S type one end is melted on the electric cautery of 100 ℃ to form to expand and hold 41, makes gallbladder intubate 4;
In embodiment, the length of straight type polyurethane conduit is 15cm, internal diameter≤1mm.
In embodiment, adopt suture that a fast knot is fixed in gallbladder model middle and lower part in step 3, and suture is No. 6 sutures.
First, at the enterprising line operate of mouse model, can effectively promote the proficiency of user, improve the success rate of live body operation.
While doing experiment on intravital mouse, method is different again, and concrete grammar comprises the following steps:
The making of step 1, gallbladder intubate 4: with length, be 15cm, internal diameter is that the gallbladder intubate 4 of making long S type structure is elongated at the two ends of the straight type polyurethane conduit of 1mm with a conduit made of iron in the water of 80 ℃, then the one end is melted on the electric cautery of 100 ℃ one expand the end 41;
step 2, the implantation of gallbladder intubate 4: with concentration expressed in percentage by weight, be that 1.5% to 5% isoflurane carries out inhalation anesthesia to mice, mice is lain on the back on the operation thermostatic platform, smear the hair of mouse web portion with suds, cut off this part hair with razor again and carry out preserved skin, after with medical alcohol sterilization skin, do longitudinal cut with scalpel, expose the liver of mice and the gallbladder of its below, after the ligation common bile duct, fully expose gallbladder, and thereunder pad a gauze, fix a fast knot with No. 6 sutures in the gallbladder middle and lower part, subsequently the end 41 that expands of gallbladder intubate 4 is implanted in the gallbladder of mice, and use immediately suture loop around fixing, after confirming that bile flows into gallbladder intubate 4 smoothly, mix up the position of gallbladder intubate 4, close abdominal cavity, and gallbladder intubate 4 is drawn from the mice cervical region through subcutaneous,
Fixing of step 3, gallbladder intubate 4: the gallbladder intubate 4 that will draw is connected and fixed with the location iron wire 3 use adhesive tapes that are fixed in the mouse carotid back;
The collection of step 4, bile: the mice that will complete 4 implantation of gallbladder intubate is put into mice rearging cage 2, and the outlet of gallbladder intubate 4 upper ends is placed in liquid storing cup 5.Appoint mice freely movable, the bile of mice gallbladder secretion flows into liquid storing cup 5 through gallbladder intubate 4, completes the collection of bile.
Fixed support 1 consists of the montant 12 that a suspension rod 11 and Duo Gen interval arrange, and can connect a mice rearging cage 2 on every montant 12, and the interior setting of mice rearging cage 2 at a draft water drum 7 is used for providing drinking-water to mice; In the bile collection process, be sprinkled into food in mice rearging cage 2, and at the interior injection drinking-water of drinking-water cylinder 7, the mice of completing 4 implant surgeries of gallbladder intubate is raised in this mice rearging cage 2, gallbladder intubate 4 is connected with sagging location iron wire 3, the 4 outlet bendings of gallbladder intubate are placed in liquid storing cup 5, and the upper end of location iron wire 3 is passed the connection tube 14 of rotating shaft 13 lower ends settings and with the suspension rod 11 of fixed support 1, is connected cooperation.Just due to the setting of rotating shaft 13, and 30 minutes softnesses of location iron wire, mice is in the mice rearging cage, and location iron wire 3 and gallbladder intubate 4 all can not affect the activity of mice, both guaranteed the normal collection of the bile of mice, do not affect again mice normal drinking-water, search for food and movable.
The setting that end 41 is expanded in gallbladder intubate 4 ends, effectively prevent mice when activity, and gallbladder intubate 4 can not extracted from gallbladder, guarantees normally carrying out of bile collection; And gallbladder intubate 4 is after iron wire 3 is connected with location, and gallbladder intubate 4 and liquid storing cup 5 all are in the top of mice rearging cage 2, touches while avoiding mice movable and affects the collection of bile.
Use mice bile gathering-device of the present invention to carry out the collection of mice bile, can be in the situation that do not affect the mice activity, carrying out long-term bile collects, when collecting bile, can also carry out real-time dynamic observation to mice, thereby obtain experimental data accurately, be used for the carrying out of research.
Most preferred embodiment of the present invention is illustrated, and various variations or the remodeling made by those of ordinary skills can not depart from the scope of the present invention.
Claims (10)
1. mice bile gathering-device, include fixed support (1), it is characterized in that: described fixed support (1) is connected with at least one bile collector unit, each described bile collector unit includes a mice rearging cage (2) and a bile gathering-device, liquid storing cup (5) and an end that described bile gathering-device includes for storage bile are connected with liquid storing cup (5), the other end is implanted the gallbladder intubate (4) of mice gallbladder, the end that described gallbladder intubate (4) is positioned at the mice gallbladder expands to be formed with and expands end (41), be connected with a vertical sagging location iron wire (3) on described gallbladder intubate (4), described location iron wire (3) one ends and fixed support (1) are flexibly connected, the other end is fixed on the cervical region of mice.
2. a kind of mice bile gathering-device according to claim 1 is characterized in that: described liquid storing cup (5) is fixedly connected with location iron wire (3).
3. a kind of mice bile gathering-device according to claim 2, it is characterized in that: described fixed support (1) consists of the montant (12) that a suspension rod (11) and interval arrange, each described bile collector unit all with montant (12) secure fit accordingly.
4. a kind of mice bile gathering-device according to claim 3, it is characterized in that: be arranged at intervals with rotating shaft (13) on described suspension rod (11), the lower end of described rotating shaft (13) is provided with connection tube (14), and described location iron wire (3) passes this connection tube (14) and is connected cooperation with it with the end that fixed support (1) is flexibly connected.
5. a kind of mice bile gathering-device according to claim 4 is characterized in that: described gallbladder intubate (4) is crooked sagging and be placed in liquid storing cup (5) in the lower end of rotating shaft (13).
6. a kind of mice bile gathering-device according to claim 5, is characterized in that: be connected and fixed by lashing wire (6) between described liquid storing cup (5) and described location iron wire (3).
7. a kind of mice bile gathering-device according to claim 6 is characterized in that: be provided with drinking-water cylinder (7) in described mice rearging cage (2), the lower end of described drinking-water cylinder (7) is provided with drinking tube (71).
8. operational approach of using mice bile gathering-device as claimed in claim 1 on mouse model is characterized in that: comprise the following steps:
Step 1, preparation mouse model, be provided with the gallbladder model in proportion in the mouse model abdomen;
Step 2, preparation gallbladder intubate (4), straight type polyurethane conduit is elongated make long S type structure in the water of 80 ℃, then the polyurethane catheter of S type one end is melted on the electric cautery of 100 ℃ to form and expand end (41), make gallbladder intubate (4);
Step 3, mouse model is lain on the back on operating board, expose the gallbladder model in the mouse model abdomen, fix a fast knot in gallbladder model middle and lower part, subsequently the end (41) that expands of gallbladder intubate (4) is implanted in the gallbladder model, and use immediately suture loop around fixing, confirm expanding after end (41) has been positioned at the gallbladder model of gallbladder intubate (4), mix up the position of gallbladder intubate (4), close the mouse model abdominal cavity, and gallbladder intubate (4) is drawn from the mouse model cervical region;
Step 4, the gallbladder intubate (4) that will draw and the location iron wire (3) that is fixed on mouse model nape section are connected and fixed;
Step 5, will complete the mouse model that gallbladder intubate (4) implants and put into mice rearging cage (2), to locate iron wire (3) is connected with the rotating shaft (13) on fixed support (1), then liquid storing cup (5) is fixed on location iron wire (3), then the outlet of gallbladder intubate (4) upper end is placed in liquid storing cup (5); Make mouse model simulation mice movable, adjust location iron wire (3) until the mouse model activity is not hindered.
9. the operational approach of using mice bile gathering-device on mouse model according to claim 8, it is characterized in that: the length of described straight type polyurethane conduit is 15cm, internal diameter internal diameter≤1mm.
10. the operational approach of using mice bile gathering-device on mouse model according to claim 8, is characterized in that: adopt suture that a fast knot is fixed in gallbladder model middle and lower part in described step 3, and described suture is No. 6 sutures.
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| CN201310340159.3A CN103385767B (en) | 2013-08-07 | 2013-08-07 | Mouse gall collecting device and method for operating mouse gall collecting device on mouse model |
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| CN201310340159.3A CN103385767B (en) | 2013-08-07 | 2013-08-07 | Mouse gall collecting device and method for operating mouse gall collecting device on mouse model |
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Citations (5)
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| US20010031932A1 (en) * | 2000-04-12 | 2001-10-18 | Blake Kevin R. | Automated blood sampling apparatus |
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2013
- 2013-08-07 CN CN201310340159.3A patent/CN103385767B/en not_active Expired - Fee Related
Patent Citations (5)
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| CN2382383Y (en) * | 1999-08-12 | 2000-06-14 | 中国人民解放军军事医学科学院放射医学研究所 | Rotary device for animal transfusion set without restrain |
| US20010031932A1 (en) * | 2000-04-12 | 2001-10-18 | Blake Kevin R. | Automated blood sampling apparatus |
| CN2462882Y (en) * | 2001-01-17 | 2001-12-05 | 屠伟峰 | Continuous transfusion system |
| CN102397114A (en) * | 2010-09-17 | 2012-04-04 | 杨建军 | Rotary continuous venous transfusing device for small animal model |
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