CN103356899A - Medicament for treating menopausal syndrome and preparation method thereof - Google Patents
Medicament for treating menopausal syndrome and preparation method thereof Download PDFInfo
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Abstract
The invention discloses a medicament for treating menopausal syndrome and a preparation method thereof. The medicament is mainly prepared from fried bighead atractylodes rhizome, dendrobium stem, oyster, glossy privet fruit, kudzu vine root, epimedium, hawthorn, mulberry and auxiliary materials, has the functions of nourishing kidney and tonifying yin, removing dryness and calming, nourishing ovarian and regulating menstruation, can be used for relieving irregular menstruation, flushed face, palpitation, insomnia, weakness, depression, hypochondriasis, unstable emotion, irritability or melancholy, concentration difficulty and other symptoms, has a treatment effect on menopausal syndrome caused by various reasons. The medicament is safe and convenient, and does not have toxic or side effect.
Description
Invention field:
The present invention relates to a kind of medicine for the treatment of climacteric syndrome and preparation method thereof, belong to the field of drug technique.
Technical background:
Climacteric syndrome (climacteric syndrome) mean since climacteric spirit psychology, neuroendocrine and metabolic alterations, the symptom of caused each tract and integrative psychological syndrome.With advancing age, the decline of women's ovarian function, sex hormones secretion reduces, and causes Gonadotropin Level to raise, and causes the hypothalamic-pituitary-ovarian axis dysequilibrium, thereby causes endocrine and autonomic nervous dysfunction.In addition, after the women entered into the climacteric period, the variation of family and social environment all can increase the weight of its body ﹠ mind burden, made climacteric syndrome be easy to occur or made original existing some sx↑.The unsettled women of some mental status, climacteric syndrome is just more obvious, even being happy and angry uncertainly.Had a strong impact on the normal live and work of patient.
Climacteric syndrome also has title " menopausal syndrome " in Chinese medicine.Because of women's year that a warp is disconnected, congenital kidney qi degradation, conception vessel is empty, and taichong channel declines, and menses will exhaust, and cause the body negative and positive to be lost rare, or deficiency of kidney yin, and sun loses hiding; Or kidney yang deficiency, passages through which vital energy circulates loses the disease that occurs a series of function disorders in replenishing vital QI with drugs of warm nature.Disease see menoxenia, flushed face, cardiopalmus, insomnia, weak, depressed, worry too much, emotional instability, irritated irritability or melancholy, attention is difficult to concentrate etc.During treatment, take the kidney invigorating gas, regulating YIN and YANG as main method.
The aging of human longevity is the important development trend of modern society.Therefore climacteric and postmenopausal women's health care and diseases prevention and treatment are vital tasks that gynecologist and even entire society face.Therefore inventing a kind of medicine that can treat climacteric syndrome has great importance.
Summary of the invention:
Technical problem to be solved by this invention is to provide a kind of medicine for the treatment of climacteric syndrome and preparation method thereof.Described medicine has replenishing kidney-YIN, except dryly calming the nerves, support the effect such as nest regulating menstruation, can effectively treat climacteric syndrome.
For solving above technical problem, the present invention realizes by the following technical solutions:
A kind of medicine for the treatment of climacteric syndrome, calculate according to composition by weight, be prepared from by 6～12 parts of Rhizoma Atractylodis Macrocephalae (parched)s, 15～30 parts of Dendrobium nobiles, 9～30 parts of Concha Ostreaes, 6～12 parts of Fructus Ligustri Lucidi, 10～15 parts of Radix Puerariaes, 6～10 parts of Herba Epimedii, 9～12 parts of Fructus Crataegis, 9～15 parts in Fructus Mori and adjuvant.
The medicine of aforesaid treatment climacteric syndrome calculates according to composition by weight, is prepared from by 10 parts of Rhizoma Atractylodis Macrocephalae (parched)s, 25 parts of Dendrobium nobiles, 20 parts of Concha Ostreaes, 10 parts of Fructus Ligustri Lucidi, 12 parts of Radix Puerariaes, 8 parts of Herba Epimedii, 10 parts of Fructus Crataegis, 12 parts in Fructus Mori and adjuvant.
The preparation method of the medicine of aforementioned therapies climacteric syndrome is: by the proportioning side of taking by weighing Raw, add conventional adjuvant, be prepared into the conventional medicine preparation according to conventional method.
Specifically, the preparation method of the medicine of aforementioned therapies climacteric syndrome is: Concha Ostreae powder is broken into fine powder, sterilizes for subsequent use; The Rhizoma Atractylodis Macrocephalae, Fructus Ligustri Lucidi and Dendrobium nobile water boiling and extraction, the extracting solution concentrating under reduced pressure becomes extractum, and drying is ground into fine powder, and is for subsequent use; Fructus Mori, Radix Puerariae, Fructus Crataegi and Herba Epimedii alcohol reflux, extracting solution Recycled ethanol and concentrating under reduced pressure become extractum, and drying is ground into fine powder, and is for subsequent use; Merge above-mentioned fine powder, add conventional adjuvant, be prepared into the conventional medicine preparation according to conventional method.
More particularly, the preparation method of the medicine of aforementioned therapies climacteric syndrome is: Concha Ostreae powder is broken into fine powder, sterilizes for subsequent use; The Rhizoma Atractylodis Macrocephalae, Fructus Ligustri Lucidi and Dendrobium nobile decoct 2.5h after soaking 30min with 6 times of water gagings, filter, filtrate-0.06～-relative density is 1.17～1.20 extractum when being evaporated to 50～60 ℃ under the 0.08Mpa condition, drying is ground into fine powder, and is for subsequent use; After Fructus Mori, Radix Puerariae, Fructus Crataegi and Herba Epimedii soak 30min first with 5 times of amount 75% ethanol, reflux, extract, 1h, add again 5 times of amount 75% ethanol extraction 1h after the filtration, filter merge extractive liquid,, Recycled ethanol and at-0.08Mpa, relative density is 1.32～1.35 extractum when being evaporated to 75 ℃ under 75 ℃ of conditions, at the vacuum of-0.04Mpa, drying under reduced pressure under 75 ℃ of conditions, be ground into fine powder, for subsequent use; Merge above-mentioned fine powder, add conventional adjuvant, be prepared into the conventional medicine preparation according to conventional method.
Described pharmaceutical preparation is capsule, tablet or granule.
Described capsule prepares like this: Concha Ostreae powder is broken into fine powder, sterilizes for subsequent use; The Rhizoma Atractylodis Macrocephalae, Fructus Ligustri Lucidi and Dendrobium nobile decoct 2.5h after soaking 30min with 6 times of water gagings, filter, filtrate-0.06～-relative density is 1.17～1.20 extractum when being evaporated to 50～60 ℃ under the 0.08Mpa condition, drying is ground into fine powder, and is for subsequent use; After Fructus Mori, Radix Puerariae, Fructus Crataegi and Herba Epimedii soak 30min first with 5 times of amount 75% ethanol, reflux, extract, 1h, add again 5 times of amount 75% ethanol extraction 1h after the filtration, filter merge extractive liquid,, Recycled ethanol and at-0.08Mpa, relative density is 1.32～1.35 extractum when being evaporated to 75 ℃ under 75 ℃ of conditions, at the vacuum of-0.04Mpa, drying under reduced pressure under 75 ℃ of conditions, be ground into fine powder, for subsequent use; Merge above-mentioned fine powder, mixing adds 25% starch of the amount of making again, the alcohol granulation with 70%, and 45 ℃ of cold drying, granulate packs 2 into
#Capsule, check is packed and be get final product.
Described tablet prepares like this: Concha Ostreae powder is broken into fine powder, sterilizes for subsequent use; The Rhizoma Atractylodis Macrocephalae, Fructus Ligustri Lucidi and Dendrobium nobile decoct 2.5h after soaking 30min with 6 times of water gagings, filter, filtrate-0.06～-relative density is 1.17～1.20 extractum when being evaporated to 50～60 ℃ under the 0.08Mpa condition, drying is ground into fine powder, and is for subsequent use; After Fructus Mori, Radix Puerariae, Fructus Crataegi and Herba Epimedii soak 30min first with 5 times of amount 75% ethanol, reflux, extract, 1h, add again 5 times of amount 75% ethanol extraction 1h after the filtration, filter merge extractive liquid,, Recycled ethanol and at-0.08Mpa, relative density is 1.32～1.35 extractum when being evaporated to 75 ℃ under 75 ℃ of conditions, at the vacuum of-0.04Mpa, drying under reduced pressure under 75 ℃ of conditions, be ground into fine powder, for subsequent use; Merge above-mentioned fine powder, use 70% alcohol granulation, add the Pulvis Talci of the amount of making 5%, 0.5% magnesium stearate mixing, with 14 mesh sieve granulate, tabletting, packing, and get final product.
Described granule prepares like this: Concha Ostreae powder is broken into fine powder, sterilizes for subsequent use; The Rhizoma Atractylodis Macrocephalae, Fructus Ligustri Lucidi and Dendrobium nobile decoct 2.5h after soaking 30min with 6 times of water gagings, filter, filtrate-0.06～-relative density is 1.17～1.20 extractum when being evaporated to 50～60 ℃ under the 0.08Mpa condition, drying is ground into fine powder, and is for subsequent use; After Fructus Mori, Radix Puerariae, Fructus Crataegi and Herba Epimedii soak 30min first with 5 times of amount 75% ethanol, reflux, extract, 1h, add again 5 times of amount 75% ethanol extraction 1h after the filtration, filter merge extractive liquid,, Recycled ethanol and at-0.08Mpa, relative density is 1.32～1.35 extractum when being evaporated to 75 ℃ under 75 ℃ of conditions, at the vacuum of-0.04Mpa, drying under reduced pressure under 75 ℃ of conditions, be ground into fine powder, for subsequent use; Merge above-mentioned fine powder, add the dextrin of the amount of making 60%, spray into 2% hydroxypropyl emthylcellulose ethanol and granulate, packing, and get final product.
The medicine for the treatment of climacteric syndrome of the present invention is made altogether by Rhizoma Atractylodis Macrocephalae (parched), Dendrobium nobile, Concha Ostreae, Fructus Ligustri Lucidi, Radix Puerariae, Herba Epimedii, Fructus Crataegi, Fructus Mori and adjuvant and is formed.Wherein: Rhizoma Atractylodis Macrocephalae (parched) has invigorating the spleen and benefiting QI, dampness diuretic, hidroschesis, antiabortive effect, is used for insufficiency of the spleen lack of appetite, phlegm retention vertigo and palpitation, spontaneous perspiration etc.; Polysaccharide component in the modern study proof Rhizoma Atractylodis Macrocephalae has the merit that strengthens immunity, antioxidation.Fructus Ligustri Lucidi can nourishing the liver and kidney, the black hair that makes eye bright, and can be used for the hepatic and renal YIN deficiency, vertigo and tinnitus, soreness of the waist and knees, interior-heat and quenches one's thirst etc.; Modern study shows that Fructus Ligustri Lucidi is one of defying age, antineoplastic Chinese medicine, and simultaneously, Fructus Ligustri Lucidi also has the effects such as hepatoprotective effect, hormonal dual regulation, blood sugar lowering.Herba Dendrobii has the effect of reinforcing stomach reg fluid, nourishing YIN and clearing away heat, is usually used in mouthfeel agitation, deficiency of stomach-Yin, hyperactivity of fire caused by deficiency of YIN, poor vision etc.; Modern study proof Herba Dendrobii has the preferably merit of defying age, antioxidation.The merit that Fructus Mori has nourishing YIN and supplementing blood, promotes the production of body fluid and moisturize often is used to the hepatic and renal YIN deficiency, vertigo and tinnitus, palpitation and insomnia etc.; Modern study shows that Fructus Mori has the effects such as defying age, enhancing immunity, hepatoprotective and blood sugar lowering.Radix Puerariae tool expelling pathogenic factors from muscles for reducing heat, promoting the production of body fluid to quench thirst, the effect such as dysmenorrhea is active is used for thirsty, dizziness and headache etc.; Studies have shown that the good resisting oxidation free radical of Radix Puerariae tool, arrhythmia, blood sugar lowering etc.Fructus Crataegi has promoting digestion and invigorating the stomach, circulation of qi promoting dissipating blood stasis, changes the merit of turbid blood fat reducing, is usually used in meat stagnation, gastral cavilty distension, congestion amenorrhea, hyperlipemia and levies etc.; Modern study proof Fructus Crataegi can increase the secretion of digestive enzyme in the stomach, and the gastrointestinal tract transporting is had facilitation, and in addition, Fructus Crataegi also has antioxidation.The merit of Herba Epimedii tool kidney-replenishing, bone and muscle strengthening and curing rheumatism is used for kidney yang deficiency, impotence and seminal emission etc.; Modern pharmacological research shows that Herba Epimedii has the effects such as protection genitals, resisting fatigue, raising memory.Concha Ostreae has tranquillization with heavy prescription, nourishing YIN YANG hyperactivity suppressing, restrains merit astringent or styptic treatment for spontaneous sweating, hard masses softening and resolving, often is used to palpitate with fear insomnia, vertigo and tinnitus, bleeding not during menses etc.; Modern study proof Concha Ostreae has blood sugar lowering, antioxidation, the anti-ageing effect of waiting for a long time.
The present invention is take Fructus Mori, Fructus Ligustri Lucidi, Herba Epimedii and Concha Ostreae the kidney invigorating gas, adjusting negative and positive as main body, the merit of the Rhizoma Atractylodis Macrocephalae, Herba Dendrobii, Radix Puerariae promoting the production of body fluid to quench thirst, invigorating the spleen and benefiting QI is assisted the purpose that reaches the treatment climacteric syndrome mutually with the digestion-promoting spleen-invigorating of Fructus Crataegi, the effect of circulation of qi promoting dissipating blood stasis again in addition.All medicines are aided each other, and treating both the principal and secondary aspects of a disease is played altogether replenishing kidney-YIN, removed the dry effect of calming the nerves, supporting the nest regulating menstruation.Studies show that, the present invention has the estrogenic secretion of increasing, relief of menstrual is uncomfortable, flushed face, cardiopalmus, insomnia, weak, depressed, worry too much, emotional instability, irritated irritability or melancholy, the symptoms such as attention is difficult to concentrate have therapeutical effect for the climacteric syndrome due to a variety of causes.
The inventor has carried out a large amount of research to medicine of the present invention, and is as follows:
Experimental example 1: technical study
Capsule of the present invention is original to adopt following method to prepare: Concha Ostreae powder is broken into fine powder, sterilizes for subsequent use; Rhizoma Atractylodis Macrocephalae (parched), Dendrobium nobile, Fructus Ligustri Lucidi, Radix Puerariae, Herba Epimedii, Fructus Crataegi and Fructus Mori decoct 2 times after soaking 30min with 8 times of water gagings, each 2h, filter, filtrate merges ,-0.06～-relative density is 1.17～1.20 extractum when being evaporated to 50～60 ℃ under the 0.08Mpa condition, adds 30% starch and Concha Ostreae powder of the amount of making again, mixing, 45 ℃ of cold drying, granulate packs 2 into
#Capsule, check is packed and be get final product.
But in clinical use, find, the medicine of the method preparation, curative effect is good not.Therefore, the inventor is studied the extraction process of product of the present invention, and is as follows:
One, extracts, concentrates and drying process research
Concha Ostreae is taken into consideration based on existing extracting method and actual production in the side, for effective utilization, is crushed into fine powder, sterilizes for subsequent use; Modern pharmacology studies show that polysaccharide has defying age and antioxidation in Rhizoma Atractylodis Macrocephalae (parched), Herba Dendrobii and the Fructus Ligustri Lucidi; Fructus Mori, Radix Puerariae, Fructus Crataegi, Herba Epimedii all contain flavones ingredient and this constituents is antioxidation, anti-ageing effective ingredient of waiting for a long time; Therefore, we carry out technical study to Rhizoma Atractylodis Macrocephalae (parched), Herba Dendrobii, Fructus Ligustri Lucidi and the Fructus Mori that contains effective composition flavonoid, Radix Puerariae, Fructus Crataegi, the Herba Epimedii that contains effective composition polysaccharide group respectively.
1. Rhizoma Atractylodis Macrocephalae (parched), Herba Dendrobii, Fructus Ligustri Lucidi Study on extraction
1.1 extract the selection of solvent
In this group medicine, its main effective ingredient is the polysaccharide composition, and its effect is antioxidation, anti-ageing waiting for a long time.Analysis-by-synthesis determines that water is to extract solvent.
1.2 the medical material water absorption is investigated
Take by weighing three parts of the medical materials such as Rhizoma Atractylodis Macrocephalae (parched), Herba Dendrobii and Fructus Ligustri Lucidi in the medical material ratio, add 5 times of water gagings and soak, filter, recording water absorption rate is 113%.The accurate throwing amount that adds water that is determined as of water absorption rate provides strong data and foundation.
Take by weighing 3 parts of medical materials according to recipe quantity, after again weighing respectively, add water to submerge respectively, the different time weighing is pressed formula and is calculated its water absorption rate.Water absorption rate=(after the medical material suction-the medical material suction is front)/medical material dry weight * 100%.A wherein: heavy before the medical material moisture absorption; Heavy after the moisture absorption of B medical material; The C water absorption rate, unit of weight is gram, the results are shown in following table:
Table 1 medical material water absorption rate investigation table
As shown in Table 1, the water absorption rate of prescription medical material is 113%.
1.3 extraction process Orthogonal Experiment and Design
Take the dendrobium polysaccharide yield as index, by the medicinal material extract principle as can be known, extraction efficiency is except outside the Pass having with the character of this life of medical material, and is also relevant with factors such as solvent species, solvent load, extraction times.See that above-mentioned definite this group medicinal material extract solvent is water, behind a large amount of preliminary experiments, choose liquid (water) material than (A), temperature (B), extraction time (C) 3 factors and 3 levels carry out positive quadraturing design test, repeats 2 times and tests randomized block design.Factor level sees Table 2, and orthonormal design of experiments the results are shown in Table 3, and variance analysis sees Table 4.
Table 2 dendrobium polysaccharide extraction process factor level table
Table 3 dendrobium polysaccharide extracts the Orthogonal experiment results table
The variance analysis of table 4 dendrobium polysaccharide extraction ratio
According to table 3 and table 4 as can be known: liquid ratio, extract temperature, extraction time the yield of dendrobium polysaccharide had utmost point appreciable impact; Optimum extraction process is combined as A
2B
3C
1, namely liquid ratio is 6:1, extracts 100 ℃ of temperature, extraction time is that the dendrobium polysaccharide of 2.5h gained is maximum.
1.4 concentrated, drying process is investigated
Extraction process by water because adopting is subjected to heat damage for a long time for preventing effective ingredient, and the concentrated required time of normal pressure is long, and thickening temperature is high, effective ingredient in the destructible prescription, concentrating under reduced pressure then have concentration time short, to the little advantage of heat-sensitive materials destruction in the prescription.In conjunction with our factory's production equipment practical situation, we select concentrating under reduced pressure to this product decocting liquid, and determine that by test of many times the parameter of concentrating under reduced pressure is :-0.06～-0.08Mpa, be concentrated into relative density and be 1.17～1.20(50～60 ℃) and dry.
The Study on extraction of 2 Fructus Moris, Radix Puerariae, Fructus Crataegi, Herba Epimedii
2.1 the selection of extractant
Effective ingredient is the large class of flavone in Fructus Mori, Radix Puerariae, Fructus Crataegi, the Herba Epimedii, and this large constituents is antioxidation, anti-ageing effective ingredient of waiting for a long time, and for effectively extracting flavones ingredient wherein, we select ethanol is to extract solvent.
2.2 extraction process is preferred
Show through a large amount of preliminary experiment results, flavonoid is few than the extraction yield of flavones ingredient in other medical materials in the Fructus Crataegi, and therefore, general flavone content is index in the selection Fructus Crataegi, with concentration of alcohol and consumption, extraction time and extraction time; Therefore with L
9(3
4) orthogonal design carries out the extraction process of total flavones in the preferred Fructus Crataegi, the factor level table sees Table 5, the results are shown in Table 6.
Table 5 quadrature factor water-glass
Table 6L
9(3
4) orthonormal design of experiments table as a result
According to Orthogonal experiment results: the optimum extraction process of total flavones is combined as A in the Fructus Crataegi
3B
1C
2D
2, namely soaking first behind the 30min with 5 times of amount 75% alcohol reflux 2 times, each 1h is optimum extraction process.
2.3 concentrated, drying process research
Because adopting ethanol for extracting solvent, for the purpose of recycling and safety, adopt first decompression recycling ethanol to without ethanol flavor, be subjected to for a long time heat damage for preventing effective ingredient, (vacuum be routine-0.08Mpa) to adopt concentrating under reduced pressure, when concentrating under 75 ℃ of conditions of thickening temperature, the consumption time is shorter, and the while is according to the character of medicine, equipment performance and operating condition, determine that the clear paste relative density is 1.32～1.35 (75 ℃), the operation of more convenient like this clear paste drying; For guaranteeing that effective ingredient is not destroyed, the final drying parameter of determining is the vacuum of employing-0.04Mpa, 75 ℃ of drying under reduced pressure.
Experimental example 2 tests of pesticide effectiveness
1 material
1.1 main medicine, instrument and reagent:
Diethylstilbestrol tablet, Guangdong Bidi Pharmaceutical Co., Ltd, lot number 20111021;
Capsule of the present invention (according to the method preparation of the embodiment of the invention 1);
(by the following method preparation: Concha Ostreae powder is broken into fine powder to former technique capsule, sterilizes for subsequent use; Rhizoma Atractylodis Macrocephalae (parched), Dendrobium nobile, Fructus Ligustri Lucidi, Radix Puerariae, Herba Epimedii, Fructus Crataegi and Fructus Mori decoct 2 times after soaking 30min with 8 times of water gagings, each 2h, filter, filtrate merges ,-0.06～-relative density is 1.17～1.20 extractum when being evaporated to 50～60 ℃ under the 0.08Mpa condition, adds 30% starch and Concha Ostreae powder of the amount of making again, mixing, 45 ℃ of cold drying, granulate packs 2 into
#Capsule, check is packed and be get final product.）；
Automatic releasing is exempted from instrument, good company in the Chinese University of Science and Technology; CR3i multifunctional table-type centrifuge, French Jouan company; Cryogenic refrigerator, Indeslt, Italian NABILA produces; MODEL, DS-67 electronic scale, Dalian Xing Hai electronic scale company limited; 125I-T radioimmunity medicine box, the Beijing North biotechnology research provides, lot number 070620.
1.2 animal: 75 of health female unpregnancy Wistar rats in 12 age in week, body weight (250 ± 10) g is purchased from animal experiment study center, Hubei Province, licence numbering SCXK (Hubei Province) 2011-0007.
2 test methods
2.1 modeling and grouping: get 75 of animals, get at random 15 as Normal group, remaining all takes Bilateral oophorectomy operation to add the folder tail to enrage the method modeling, is divided at random model group, diethylstilbestrol group, of the present invention group after the modeling success, former technique group, 15 every group.
Modeling method: press 4ml/kg body weight lumbar injection 0.8% pentobarbital solution.Anesthesia venter posterior position is fixed.Get under the last rib, midaxillary line and apart from the spinal column outside about 2cm infall, unhairing, sterilization skin cuts skin and dorsal muscles, otch is about 1.5cm-2cm, the ovary of visible pink, reddish yellow.First fallopian tube under the ovary (comprising liparitosis) is pricked with toe-in, extractd again ovary; Extract the opposite side ovary with method, make kidney qi virtual loss disease model.The postoperative routine gives penicillin 200,000 U lumbar injections, every day 1 time, continuously 5d.6d begins all tested rats are carried out vaginal smear, places microscopically to carry out vaginal cytology, continuous 5d, and vaginal exfoliated cardiac index,contrast index,cornification index＜50%, and without oestrous cycle property variation person, be the modeling success of suffering from a deficiency of the kidney.11d rises and all modeling rats are pressed from both sides tail again enrages stimulation, makes depression of liver-QI.Do not have, continuously 7d days for 2 times.When modeling finishes, occur losing weight, hair is dimmed, bradykinesia etc., reduce with drinking-water, appetite simultaneously, eyes are narrowed little, caudal style, sonic stimulation reacts without Vigilance to external world, hides the Behavioral feature that attitude meets liver depression if external irritant is, so far on the kidney qi loss basis, enrage the method stimulation by the folder tail and cause depression of liver-QI, climacteric syndrome modeling success is described.
2.2 medicine preparation, dosage and sample collection: diethylstilbestrol tablet faces the time spent and is made into the aqueous solution that concentration is 0.0045mg/ml.The present invention combines the aqueous solution that concentration is 16.2mg/ml.Former technique combines the aqueous solution that concentration is 16.2mg/ml.Each organizes experimental animal all with 1ml/100g body weight difference gavage, every day 1 time.Continuously 4 weeks of gavage, after finishing, last puts to death behind the fasting 24h.Broken end is got blood fast, collects 5ml blood in being on the waiting list serum in the ready test tube first; All sample collections are placed in 4 ℃ of environment essence and put behind the 2h centrifugally, and centrifugal rear separation of serum is sub-packed in the plastic centrifuge tube of 1.5ml, preserves standby the survey in-20 ℃ of cryogenic refrigerators.
2.3 index determining method: detect and adopt radio immunoassay to measure E in the serum
2, T, FSH content.The test kit rule of operation is carried out according to appended description.
3 results
Compare model group rat blood serum E with Normal group
2, the T level obviously reduces (p＜0.01=, the FSH level (p＜0.01=that obviously raises.Compare of the present invention group and diethylstilbestrol group rat blood serum E with model group
2, the T level (p＜0.05=, and the FSH level obviously reduces (p＜0.05=that obviously raises.The results are shown in Table 7.
Table 7 is respectively organized rat blood serum E
2, T, FSH content relatively (n=15, x ± s)
Annotate: compare with normal group:
*P＜0.01;
Compare with model group:
#P＜0.05;
##P＜0.01;
Compare with former technique group:
▲P＜0.05.
4 conclusions and discussion
At present, the pathogenesis of climacteric syndrome is not yet fully clear, but a large amount of Clinical and experimental studies shows, climacteric syndrome patients serum E2, T level are lower than the climacteric asymptomatic person, FSH illustrates that then apparently higher than asymptomatic person estrogenic excessive minimizing is the pathogenesis basis of climacteric syndrome.Therefore, observe the variation of E2, T, FSH content, in the diagnosis and treatment of climacteric syndrome, very important meaning is arranged.
Can find out from this experimental result, after the rats underwent Bilateral oophorectomy added that the folder tail enrages modeling, its serum E2, T level were starkly lower than Normal group, and the FSH level conforms to endocrine alteration after the human climacteric menopause apparently higher than Normal group.Of the present invention group of rat blood serum E2, T level raise (P＜0.05) than model group, and FSH reduces (P＜0.05) than model group, illustrate that medicine of the present invention can regulate the multinomial reproductive endocrine value of rat model.This shows, medicine of the present invention can pass through effectively to regulate the multinomial reproductive endocrine value of rat model, thereby plays the effect for the treatment of climacteric syndrome by improving estrogenic secretion.
In addition, of the present invention group and the comparison of former technique group, rat blood serum E2, T level raise (P＜0.05), and FSH reduces (P＜0.05) than model group, illustrates that of the present invention group of medicine is than the better efficacy of former technique group medicine.
Further specify by the following examples the present invention, but not as limitation of the present invention.
The specific embodiment:
Embodiment 1
Prescription: Rhizoma Atractylodis Macrocephalae (parched) 10g, Dendrobium nobile 25g, Concha Ostreae 20g, Fructus Ligustri Lucidi 10g, Radix Puerariae 12g, Herba Epimedii 8g, Fructus Crataegi 10g, Fructus Mori 12g.
Technique: Concha Ostreae powder is broken into fine powder, sterilizes for subsequent use; The Rhizoma Atractylodis Macrocephalae, Fructus Ligustri Lucidi and Herba Dendrobii decoct 2.5h after soaking 30min with 6 times of water gagings, filter, filtrate-0.06～-relative density is 1.17～1.20 extractum when being evaporated to 50～60 ℃ under the 0.08Mpa condition, drying is ground into fine powder, and is for subsequent use; After Fructus Mori, Radix Puerariae, Fructus Crataegi and Herba Epimedii soak 30min first with 5 times of amount 75% ethanol, reflux, extract, 1h, add again 5 times of amount 75% ethanol extraction 1h after the filtration, filter merge extractive liquid,, Recycled ethanol and at-0.08Mpa, relative density is 1.32～1.35 extractum when being evaporated to 75 ℃ under 75 ℃ of conditions, at the vacuum of-0.04Mpa, drying under reduced pressure under 75 ℃ of conditions, be ground into fine powder, for subsequent use; Merge above-mentioned fine powder, mixing adds 25% starch of the amount of making again, the alcohol granulation with 70%, and 45 ℃ of cold drying, granulate, the 2# capsule of packing into, check is packed and be get final product.
Usage and dosage: oral, 3 times on the one, one time 2.
Embodiment 2
Prescription: Rhizoma Atractylodis Macrocephalae (parched) 12g, Dendrobium nobile 30g, Concha Ostreae 30g, Fructus Ligustri Lucidi 12g, Radix Puerariae 15g, Herba Epimedii 10g, Fructus Crataegi 12g, Fructus Mori 15g.
Technique: Concha Ostreae powder is broken into fine powder, sterilizes for subsequent use; The Rhizoma Atractylodis Macrocephalae, Fructus Ligustri Lucidi and Dendrobium nobile decoct 2.5h after soaking 30min with 6 times of water gagings, filter, filtrate-0.06～-relative density is 1.17～1.20 extractum when being evaporated to 50～60 ℃ under the 0.08Mpa condition, drying is ground into fine powder, and is for subsequent use; After Fructus Mori, Radix Puerariae, Fructus Crataegi and Herba Epimedii soak 30min first with 5 times of amount 75% ethanol, reflux, extract, 1h, add again 5 times of amount 75% ethanol extraction 1h after the filtration, filter merge extractive liquid,, Recycled ethanol and at-0.08Mpa, relative density is 1.32～1.35 extractum when being evaporated to 75 ℃ under 75 ℃ of conditions, at the vacuum of-0.04Mpa, drying under reduced pressure under 75 ℃ of conditions, be ground into fine powder, for subsequent use; Merge above-mentioned fine powder, use 70% alcohol granulation, add the Pulvis Talci of the amount of making 5%, 0.5% magnesium stearate mixing, with 14 mesh sieve granulate, tabletting, every 0.5g, packing, and get final product.
Usage and dosage: oral, 3 times on the one, one time 2.
Embodiment 3
Prescription: Rhizoma Atractylodis Macrocephalae (parched) 6g, Dendrobium nobile 15g, Concha Ostreae 9g, Fructus Ligustri Lucidi 6g, Radix Puerariae 10g, Herba Epimedii 6g, Fructus Crataegi 9g, Fructus Mori 9g.
Technique: Concha Ostreae powder is broken into fine powder, sterilizes for subsequent use; The Rhizoma Atractylodis Macrocephalae, Fructus Ligustri Lucidi and Dendrobium nobile decoct 2.5h after soaking 30min with 6 times of water gagings, filter, filtrate-0.06～-relative density is 1.17～1.20 extractum when being evaporated to 50～60 ℃ under the 0.08Mpa condition, drying is ground into fine powder, and is for subsequent use; After Fructus Mori, Radix Puerariae, Fructus Crataegi and Herba Epimedii soak 30min first with 5 times of amount 75% ethanol, reflux, extract, 1h, add again 5 times of amount 75% ethanol extraction 1h after the filtration, filter merge extractive liquid,, Recycled ethanol and at-0.08Mpa, relative density is 1.32～1.35 extractum when being evaporated to 75 ℃ under 75 ℃ of conditions, at the vacuum of-0.04Mpa, drying under reduced pressure under 75 ℃ of conditions, be ground into fine powder, for subsequent use; Merge above-mentioned fine powder, add the dextrin of the amount of making 60%, spray into 2% hydroxypropyl emthylcellulose ethanol and granulate, packing, and get final product.
Usage and dosage: boiled water is taken after mixing it with water, and 3 times on the one, a 10g.
Claims (9)
1. medicine for the treatment of climacteric syndrome, it is characterized in that: calculate according to composition by weight, be prepared from by 6～12 parts of Rhizoma Atractylodis Macrocephalae (parched)s, 15～30 parts of Dendrobium nobiles, 9～30 parts of Concha Ostreaes, 6～12 parts of Fructus Ligustri Lucidi, 10～15 parts of Radix Puerariaes, 6～10 parts of Herba Epimedii, 9～12 parts of Fructus Crataegis, 9～15 parts in Fructus Mori and adjuvant.
2. treat as claimed in claim 1 the medicine of climacteric syndrome, it is characterized in that: calculate according to composition by weight, be prepared from by 10 parts of Rhizoma Atractylodis Macrocephalae (parched)s, 25 parts of Dendrobium nobiles, 20 parts of Concha Ostreaes, 10 parts of Fructus Ligustri Lucidi, 12 parts of Radix Puerariaes, 8 parts of Herba Epimedii, 10 parts of Fructus Crataegis, 12 parts in Fructus Mori and adjuvant.
3. treat as claimed in claim 1 or 2 the preparation method of the medicine of climacteric syndrome, it is characterized in that: by the proportioning side of taking by weighing Raw, add conventional adjuvant, be prepared into the conventional medicine preparation according to conventional method.
4. treat as claimed in claim 3 the preparation method of the medicine of climacteric syndrome, it is characterized in that: Concha Ostreae powder is broken into fine powder, sterilizes for subsequent use; The Rhizoma Atractylodis Macrocephalae, Fructus Ligustri Lucidi and Dendrobium nobile water boiling and extraction, the extracting solution concentrating under reduced pressure becomes extractum, and drying is ground into fine powder, and is for subsequent use; Fructus Mori, Radix Puerariae, Fructus Crataegi and Herba Epimedii alcohol reflux, extracting solution Recycled ethanol and concentrating under reduced pressure become extractum, and drying is ground into fine powder, and is for subsequent use; Merge above-mentioned fine powder, add conventional adjuvant, be prepared into the conventional medicine preparation according to conventional method.
5. treat as claimed in claim 4 the preparation method of the medicine of climacteric syndrome, it is characterized in that: Concha Ostreae powder is broken into fine powder, sterilizes for subsequent use; The Rhizoma Atractylodis Macrocephalae, Fructus Ligustri Lucidi and Dendrobium nobile decoct 2.5h after soaking 30min with 6 times of water gagings, filter, filtrate-0.06～-relative density is 1.17～1.20 extractum when being evaporated to 50～60 ℃ under the 0.08Mpa condition, drying is ground into fine powder, and is for subsequent use; After Fructus Mori, Radix Puerariae, Fructus Crataegi and Herba Epimedii soak 30min first with 5 times of amount 75% ethanol, reflux, extract, 1h, add again 5 times of amount 75% ethanol extraction 1h after the filtration, filter merge extractive liquid,, Recycled ethanol and at-0.08Mpa, relative density is 1.32～1.35 extractum when being evaporated to 75 ℃ under 75 ℃ of conditions, at the vacuum of-0.04Mpa, drying under reduced pressure under 75 ℃ of conditions, be ground into fine powder, for subsequent use; Merge above-mentioned fine powder, add conventional adjuvant, be prepared into the conventional medicine preparation according to conventional method.
6. such as the preparation method of the medicine for the treatment of climacteric syndrome as described in each among the claim 3-5, it is characterized in that: described pharmaceutical preparation is capsule, tablet or granule.
7. treat as claimed in claim 6 the preparation method of the medicine of climacteric syndrome, it is characterized in that: described capsule prepares like this: Concha Ostreae powder is broken into fine powder, sterilizes for subsequent use; The Rhizoma Atractylodis Macrocephalae, Fructus Ligustri Lucidi and Dendrobium nobile decoct 2.5h after soaking 30min with 6 times of water gagings, filter, filtrate-0.06～-relative density is 1.17～1.20 extractum when being evaporated to 50～60 ℃ under the 0.08Mpa condition, drying is ground into fine powder, and is for subsequent use; After Fructus Mori, Radix Puerariae, Fructus Crataegi and Herba Epimedii soak 30min first with 5 times of amount 75% ethanol, reflux, extract, 1h, add again 5 times of amount 75% ethanol extraction 1h after the filtration, filter merge extractive liquid,, Recycled ethanol and at-0.08Mpa, relative density is 1.32～1.35 extractum when being evaporated to 75 ℃ under 75 ℃ of conditions, at the vacuum of-0.04Mpa, drying under reduced pressure under 75 ℃ of conditions, be ground into fine powder, for subsequent use; Merge above-mentioned fine powder, mixing adds 25% starch of the amount of making again, the alcohol granulation with 70%, and 45 ℃ of cold drying, granulate, the 2# capsule of packing into, check is packed and be get final product.
8. treat as claimed in claim 6 the preparation method of the medicine of climacteric syndrome, it is characterized in that: described tablet prepares like this: Concha Ostreae powder is broken into fine powder, sterilizes for subsequent use; The Rhizoma Atractylodis Macrocephalae, Fructus Ligustri Lucidi and Dendrobium nobile decoct 2.5h after soaking 30min with 6 times of water gagings, filter, filtrate-0.06～-relative density is 1.17～1.20 extractum when being evaporated to 50～60 ℃ under the 0.08Mpa condition, drying is ground into fine powder, and is for subsequent use; After Fructus Mori, Radix Puerariae, Fructus Crataegi and Herba Epimedii soak 30min first with 5 times of amount 75% ethanol, reflux, extract, 1h, add again 5 times of amount 75% ethanol extraction 1h after the filtration, filter merge extractive liquid,, Recycled ethanol and at-0.08Mpa, relative density is 1.32～1.35 extractum when being evaporated to 75 ℃ under 75 ℃ of conditions, at the vacuum of-0.04Mpa, drying under reduced pressure under 75 ℃ of conditions, be ground into fine powder, for subsequent use; Merge above-mentioned fine powder, use 70% alcohol granulation, add the Pulvis Talci of the amount of making 5%, 0.5% magnesium stearate mixing, with 14 mesh sieve granulate, tabletting, packing, and get final product.
9. treat as claimed in claim 6 the preparation method of the medicine of climacteric syndrome, it is characterized in that: described granule prepares like this: Concha Ostreae powder is broken into fine powder, sterilizes for subsequent use; The Rhizoma Atractylodis Macrocephalae, Fructus Ligustri Lucidi and Dendrobium nobile decoct 2.5h after soaking 30min with 6 times of water gagings, filter, filtrate-0.06～-relative density is 1.17～1.20 extractum when being evaporated to 50～60 ℃ under the 0.08Mpa condition, drying is ground into fine powder, and is for subsequent use; After Fructus Mori, Radix Puerariae, Fructus Crataegi and Herba Epimedii soak 30min first with 5 times of amount 75% ethanol, reflux, extract, 1h, add again 5 times of amount 75% ethanol extraction 1h after the filtration, filter merge extractive liquid,, Recycled ethanol and at-0.08Mpa, relative density is 1.32～1.35 extractum when being evaporated to 75 ℃ under 75 ℃ of conditions, at the vacuum of-0.04Mpa, drying under reduced pressure under 75 ℃ of conditions, be ground into fine powder, for subsequent use; Merge above-mentioned fine powder, add the dextrin of the amount of making 60%, spray into 2% hydroxypropyl emthylcellulose ethanol and granulate, packing, and get final product.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104474288A (en) * | 2014-11-20 | 2015-04-01 | 张进良 | Medicine for nourishing yin and promoting sleeping and preparation method thereof |
| CN105688088A (en) * | 2016-04-03 | 2016-06-22 | 马兆峰 | Pharmaceutical preparation for treating climacteric depression syndrome |
| CN110237161A (en) * | 2019-05-10 | 2019-09-17 | 广东省中医院（广州中医药大学第二附属医院、广州中医药大学第二临床医学院、广东省中医药科学院） | Drug and its preparation method and application for treating menopausal syndrome |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1308879A (en) * | 2000-12-04 | 2001-08-22 | 薛伟华 | Health-care tea and its preparing process |
| CN1785346A (en) * | 2004-12-30 | 2006-06-14 | 贵州恒霸药业有限责任公司 | Health protection capsule suitable for climateric woman |
-
2013
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Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1308879A (en) * | 2000-12-04 | 2001-08-22 | 薛伟华 | Health-care tea and its preparing process |
| CN1785346A (en) * | 2004-12-30 | 2006-06-14 | 贵州恒霸药业有限责任公司 | Health protection capsule suitable for climateric woman |
Non-Patent Citations (2)
| Title |
|---|
| 李继凤: "妇女更年期综合征中医治疗近况", 《山东中医学院学报》 * |
| 金香兰: "中医治疗女性更年期综合征的处方规律探讨", 《中国中医基础医学杂志》 * |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104474288A (en) * | 2014-11-20 | 2015-04-01 | 张进良 | Medicine for nourishing yin and promoting sleeping and preparation method thereof |
| CN105688088A (en) * | 2016-04-03 | 2016-06-22 | 马兆峰 | Pharmaceutical preparation for treating climacteric depression syndrome |
| CN110237161A (en) * | 2019-05-10 | 2019-09-17 | 广东省中医院（广州中医药大学第二附属医院、广州中医药大学第二临床医学院、广东省中医药科学院） | Drug and its preparation method and application for treating menopausal syndrome |
| CN110237161B (en) * | 2019-05-10 | 2021-07-20 | 广东省中医院（广州中医药大学第二附属医院、广州中医药大学第二临床医学院、广东省中医药科学院） | Medicine for treating menopausal syndrome and preparation method and application thereof |
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