CN103355284A - Ice warm Organ Preservation Solution - Google Patents
Ice warm Organ Preservation Solution Download PDFInfo
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- CN103355284A CN103355284A CN2013103433630A CN201310343363A CN103355284A CN 103355284 A CN103355284 A CN 103355284A CN 2013103433630 A CN2013103433630 A CN 2013103433630A CN 201310343363 A CN201310343363 A CN 201310343363A CN 103355284 A CN103355284 A CN 103355284A
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- CN
- China
- Prior art keywords
- radix astragali
- solution
- mannitol
- organ preservation
- water
- Prior art date
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Abstract
The invention discloses the warm Organ Preservation Solution of a kind of ice, the sterile water solution that is mixed by potassium citrate, sodium citrate, magnesium sulfate, mannitol, 6-aminoadenine phosphate, the Radix Astragali and water for injection, wherein every 100ml ices that to contain potassium citrate in the warm Organ Preservation Solution be 0.87～1.16g; Sodium citrate is 0.98～1.19g; Magnesium sulfate is 1.02～1.15g; Mannitol is 5.56～6.85g; 6-aminoadenine phosphate is 0.45g; Radix Astragali extractive solution 5ml after the mentioned component mixing, injects in the 100ml volumetric flask and is diluted with water to 100ml.The present invention has increased the mannitol ratio, can reduce protection liquid phase temperature point, is more suitable for Yu Bingwen and preserves.Adding the Radix Astragali has the non-oxidizability damage and promotes Na
+-K
+The effect of-atpase activity, and viscosity is low, but make the organ fast cooling in the short time, improved perfusion effect.
Description
Technical field
The invention belongs to the protective liquid that organ is preserved, be specifically related to a kind of Organ Preservation Solution that is applicable to ice under the Wen Wendu.
Background technology
It is one of three large technology pillars of organ transplant that organ is preserved.Because the appearance of Organ Preservation Solution makes over nearly 10 years the organ Techniques of preserving obtain huge advance made, desirable preservation liquid can provide suitable microenvironment for isolated organ, prolongs to greatest extent the organ holding time, is the focus of filed of organ transplantation research always.The most frequently used is the UW liquid (University of Wisconsin Solution) of being developed by University of Wisconsin clinically.Owing to this preservation liquid possesses the organ preservative fluid that good clinical and experiment preservation effect is considered to standard, but its price is comparatively expensive, has limited its extensive use of filed of organ transplantation at home and abroad.Effectively cryopreservation solution must possess following condition: (1) reduces the edema that causes because of low temperature; (2) prevent the acidization of cell; (3) prevent from protecting liquid to expand in the cold space between cells that causes in the process that charges; (4) can provide regeneration high energy phosphate compound substrate.Although UW liquid satisfies above condition substantially, except complicated component, expensive, and have that the liquid viscosity is high, the organ cooling is slow, buffer capacity is poor, K
+The defectives such as content is higher.For this reason domesticly also develop a series of Organ Preservation Solutions, but the preservation adaptive temperature of above-mentioned protection liquid is all 0～4 ℃ of scope, and all gets in use its upper limit.
As everyone knows, temperature is lower preserves more favourablely to biology, but excessively low temperature makes again microtubule bindin (MAP) lose ability in conjunction with microtubule, makes the lipid of cell membrane change gel state into by liquid crystal state, cause cell membrane fluidity to descend, destroyed the inside and outside osmotic pressure balance of cell.Therefore how controlling freezing point moves down the expansion temperature range, avoids simultaneously causing cell death by the damage of the external worlds such as physics, chemistry and biological factor, just becomes the difficult point of novel Organ Preservation Solution.In organ preservative fluid, add an amount of traditional Chinese medicine ingredients, make it help to keep cell membrane integrity and energy supply to avoid the ischemic cellular damage, become the key of Organ Preservation Solution research.
Summary of the invention
The objective of the invention is, a kind of ice temperature Organoprotective liquid that traditional Chinese medicine ingredients can reduce again phase point temperature simultaneously that is added with is provided.
The present invention is achieved by following technical proposals.Icing warm Organ Preservation Solution is the sterile water solution that is mixed by potassium citrate, sodium citrate, magnesium sulfate, mannitol, 6-aminoadenine phosphate, the Radix Astragali and water for injection, and wherein every 100ml ices that to contain potassium citrate in the warm Organ Preservation Solution be 0.87～1.16g; Sodium citrate is 0.98～1.19g; Magnesium sulfate is 1.02～1.15g; Mannitol is 5.56～6.85g; 6-aminoadenine phosphate is 0.45g; Radix Astragali extractive solution 5ml after the mentioned component mixing, injects in the 100ml volumetric flask and is diluted with water to 100ml.
Low temperature is the essential condition that the organ time-delay is preserved, and oxygen and various metabolite supply were ended after organ exsomatized, and mainly caused the Na on the cell membrane
+-K
+-ATP enzyme changes and causes cell impaired.So low temperature can reduce cellular metabolism speed as much as possible, strengthen cell to the tolerance of ischemic.The main pharmaceutical component of the Radix Astragali is astragalus flavonid and total saponins in astragalus, and astragalus flavonid has the effect of removing oxygen radical in vivo, adds total saponins in astragalus in Organ Preservation Solution, then plays the non-oxidizability damage and promotes Na
+-K
+The effect of-atpase activity.
Characteristics of the present invention and beneficial effect are:
(1) increases the mannitol ratio, can reduce protection liquid phase temperature point, be more suitable for Yu Bingwen and preserve;
(2) adding the Radix Astragali has the non-oxidizability damage and promotes Na
+-K
+The effect of-atpase activity;
(3) viscosity is low, but makes the organ fast cooling in the short time, improves perfusion effect;
(4) prescription is simple, and is cheap.
Embodiment
By the following examples formulation principle of the present invention and preparation method are described further.
Ice warm Organ Preservation Solution, by potassium citrate (C
6H
5O
7K
3), sodium citrate (C
6H
5O
7Na
3), magnesium sulfate (MgSO
47H
2O), mannitol (C
6H
14O
6), 6-aminoadenine phosphate (C
5H
5N
5H
3PO
4), the Radix Astragali and water for injection is mixed and made into sterile water solution.Wherein every 100ml ices that to contain potassium citrate in the warm Organ Preservation Solution be 0.87～1.16g; Sodium citrate is 0.98～1.19g; Magnesium sulfate is 1.02～1.15g; Mannitol is 5.56～6.85g; 6-aminoadenine phosphate is 0.45g; Radix Astragali extractive solution 5ml after the mentioned component mixing, injects in the 100ml volumetric flask and is diluted with water to 100ml.
Radix Astragali extractive solution water alcohol extracting method is made, and every milliliter contains 2.5g Radix Astragali crude drug.Used magnesium sulfate is 7 water magnesium sulfates.
Preparation method of the present invention is that with fully dissolving in above-mentioned each composition adding water for injection, Radix Astragali extractive solution directly adds.Prepare rear liquid through 109 ℃, the 45min autoclave sterilization, the pH value of obtaining liq is: 7.20 ± 0.04, osmotic pressure is: 340mOsm/L.
Following embodiment is take the animal Ren sus domestica as conservation object.Its method for filling is: pressurize through gravity with 1 ℃ of Organ Preservation Solution and fill with punching, filling with swash of wave amount is 2.7ml/s, fills with to rush when the protection liquid temp of kidney vein outflow is 3.0 ℃ to stop; Filling places 0 ± 0.15 ℃ insulating box to preserve in kidney after finishing.The biochemical index of icing warm Organ Preservation Solution different formulations and different holding time kidneys is as shown in table 1.RCR represents the kidney mitochondria respiratoring control rate in the table.
Embodiment 1: every 100ml ices that to contain potassium citrate in the warm Organ Preservation Solution be 0.9g; Sodium citrate is 1.0g; Magnesium sulfate is 1.05g; Mannitol is 5.58g; 6-aminoadenine phosphate is 0.45g; Radix Astragali extractive solution 5ml after the mentioned component mixing, injects in the 100ml volumetric flask and is diluted with water to 100ml.
Embodiment 2: every 100ml ices that to contain potassium citrate in the warm Organ Preservation Solution be 1.0g; Sodium citrate is 1.05g; Magnesium sulfate is 1.1g; Mannitol is 6.3g; 6-aminoadenine phosphate is 0.45g; Radix Astragali extractive solution 5ml after the mentioned component mixing, injects in the 100ml volumetric flask and is diluted with water to 100ml.
Embodiment 3: every 100ml ices that to contain potassium citrate in the warm Organ Preservation Solution be 1.15g; Sodium citrate is 1.18; Magnesium sulfate is 1.15g; Mannitol is 6.8g; 6-aminoadenine phosphate is 0.45g; Radix Astragali extractive solution 5ml after the mentioned component mixing, injects in the 100ml volumetric flask and is diluted with water to 100ml.
The kidney biochemical index of three embodiment of table 1
| Group | Holding time | ATP | Na +2K +2ATPase | Ca 2+ | RCR |
| ? | h | mB·nmol -1·mg -1 | (z/m)/μ?mol·h -1·mg -1 | mB·nmol -1·mg -1 | % |
| Embodiment 1 | 48 | 2.13±0.49 | 38.25±4.01 | 171.30±4.12 | 4.26±1.01 |
| ? | 72 | 1.70±0.38 | 33.67±4.49 | 195.50±3.41 | 3.93±1.00 |
| Embodiment 2 | 48 | 2.60±0.57 | 44.10±5.01 | 139.60±3.81 | 4.98±1.10 |
| ? | 72 | 1.98±0.38 | 40.35±4.69 | 184.50±5.12 | 4.74±1.04 |
| Embodiment 3 | 48 | 3.21±0.80 | 46.98±4.75 | 150.10±5.01 | 5.07±1.10 |
| ? | 72 | 3.01±0.76 | 44.00±5.18 | 179.20±5.57 | 5.24±1.03 |
Adopt the statistics t method of inspection, represent with mean standard deviation.Can find out that the data that three embodiment record compare, P＞0.05, illustrating does not have difference.
Claims (2)
1. ice warm Organ Preservation Solution, comprise potassium citrate (C
6H
5O
7K
3), sodium citrate (C
6H
5O
7Na
3), magnesium sulfate (MgSO47H2O), mannitol (C
6H
14O
6), 6-aminoadenine phosphate (C
5H
5N
5H
3PO
4) and the Radix Astragali, it is characterized in that: be mixed and made into sterile water solution by potassium citrate, sodium citrate, magnesium sulfate, mannitol, 6-aminoadenine phosphate, the Radix Astragali and water for injection, wherein every 100ml ices that to contain potassium citrate in the warm Organ Preservation Solution be 0.87～1.16g; Sodium citrate is 0.98～1.19g; Magnesium sulfate is 1.02～1.15g; Mannitol is 5.56～6.85g; 6-aminoadenine phosphate is 0.45g; Radix Astragali extractive solution 5ml after the mentioned component mixing, injects in the 100ml volumetric flask and is diluted with water to 100ml.
2. according to ice temperature Organ Preservation Solution claimed in claim 1, it is characterized in that described Radix Astragali extractive solution water alcohol extracting method makes, every milliliter contains 2.5g Radix Astragali crude drug.
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|---|---|---|---|
| CN2013103433630A CN103355284A (en) | 2013-08-07 | 2013-08-07 | Ice warm Organ Preservation Solution |
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|---|---|---|---|
| CN2013103433630A CN103355284A (en) | 2013-08-07 | 2013-08-07 | Ice warm Organ Preservation Solution |
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Family
ID=49358238
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113812397A (en) * | 2021-09-30 | 2021-12-21 | 佛山市第一人民医院（中山大学附属佛山医院） | Perfusion preservation liquid |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1633848A (en) * | 2004-12-07 | 2005-07-06 | 中国人民解放军第二军医大学 | Kidney preservative fluid |
| CN101496512A (en) * | 2008-02-01 | 2009-08-05 | 扬子江药业集团上海海尼药业有限公司 | Organ preservative fluid and preparation method thereof |
-
2013
- 2013-08-07 CN CN2013103433630A patent/CN103355284A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1633848A (en) * | 2004-12-07 | 2005-07-06 | 中国人民解放军第二军医大学 | Kidney preservative fluid |
| CN101496512A (en) * | 2008-02-01 | 2009-08-05 | 扬子江药业集团上海海尼药业有限公司 | Organ preservative fluid and preparation method thereof |
Non-Patent Citations (3)
| Title |
|---|
| 原春辉等: "黄芪在犬肾离体灌注保存中的作用", 《中华实验外科杂志》, vol. 20, no. 2, 8 February 2003 (2003-02-08), pages 151 - 152 * |
| 周智华等: "川芎嗪改善高渗枸橼酸盐嘌呤溶液", 《中华器官移植杂志》, vol. 27, no. 7, 31 December 2006 (2006-12-31), pages 411 - 413 * |
| 鲁可权: "心脏保存液研究进展", 《山东医药》, vol. 44, no. 33, 31 December 2004 (2004-12-31), pages 68 - 69 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113812397A (en) * | 2021-09-30 | 2021-12-21 | 佛山市第一人民医院（中山大学附属佛山医院） | Perfusion preservation liquid |
| CN113812397B (en) * | 2021-09-30 | 2023-03-14 | 佛山市第一人民医院（中山大学附属佛山医院） | Perfusion preserving fluid |
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Application publication date: 20131023 |