CN103344729A - Method for identifying authenticity and evaluating quality of Fengjie navel oranges - Google Patents
Method for identifying authenticity and evaluating quality of Fengjie navel oranges Download PDFInfo
- Publication number
- CN103344729A CN103344729A CN2013102961174A CN201310296117A CN103344729A CN 103344729 A CN103344729 A CN 103344729A CN 2013102961174 A CN2013102961174 A CN 2013102961174A CN 201310296117 A CN201310296117 A CN 201310296117A CN 103344729 A CN103344729 A CN 103344729A
- Authority
- CN
- China
- Prior art keywords
- fengjie
- navel orange
- navel
- quality
- chromatographic
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Images
Abstract
The invention relates to a method for identifying authenticity and evaluating quality of Fengjie navel oranges. The method comprises the following steps of: (1) sampling; (2) preparing a test solution; (3) performing high performance liquid chromatography (HPLC) analysis; and (4) identifying authenticity and evaluating quality. According to the method, according to the characteristics of composition and relative content of characteristic active ingredient flavone in Fengjie navel orange fruits, the Fengjie navel oranges are taken as a whole, chromatographic fingerprint of the characteristic ingredient of flavone in the Fengjie navel orange is established, the quality detection, monitoring and quality evaluation of the commercially available Fengjie navel oranges are realized by taking the chromatographic fingerprint as reference, the authenticity, producing areas and quality of the Fengjie navel orange fruits are identified and controlled, the defect that the accuracy is low due to the operation of identifying the navel oranges by means of the producing areas, fruit shape index and taste characteristics only is overcome, and a reliable basis is provided for solving the problem of mixed quality of local high-quality sweet orange variety in China.
Description
Technical field
The present invention relates to a kind of finger-print of sweet orange kind, particularly the method for a kind of Fengjie navel orange authenticity and quality evaluation.
Background technology
Oranges and tangerines are the big fruit tree crops of the first in the world, and mandarine cultivated area and output occupy first of the world, but local famous-brand and high-quality feature breed is less.Fengjie navel orange (Citrus sinensis Osbeck) is the excellent strain of Fengjie, Chongqing Washington navel bud mutation, is one of local famous-object of China's high-quality sweet orange.In recent years along with the expansion of mandarine cultivated area, the Fengjie navel orange is subjected to peripheral producing region puppet to emit the fruit shock effect serious.Be protection " Fengjie navel orange " brand; the authentication of " Fengjie navel orange " geographical sign product protection has been carried out in the Fengjie County; the navel orange of 20 small towns such as thatched cottage, Fengjie County town, Bai Di town, Fenhe River town now being had jurisdiction over administrative region production gives the geographical sign product protection, but the puppet that the place of production authenticates in the still fubaritic market circulation is emitted fruit.In addition, people also attempt according to fruit shape index and mouthfeel feature the Fengjie navel orange to be identified, but its index can't quantize, human factor is big, is difficult to effectively qualitatively judge.Therefore based on himself qualitative characteristics, setting up effective authenticity and method for evaluating quality, is unique channel and the outlet of the local high-quality sweet orange brand of protection.
Sweet orange fruit is nutritious, is rich in multiple compositions such as flavonoids, limonin, amino acid, vitamin and trace element, and wherein flavones is a most important active component in the sweet orange fruit.People have carried out broad research to citrusfruit flavonoids component in recent years, from different kind citrusfruits, separated at present and identified 60 various active flavones monomers, people also contrast and detect the sample flavones between the different places of production with same breed between different oranges and tangerines kinds simultaneously, the result show between different oranges and tangerines kinds and same breed under different weather conditions, the composition of citrusfruit flavones ingredient, relative content and biologically active all have notable difference.Therefore, according to the composition of fruit flavonoids characteristic chemical constituent under different cultivars or the different weather conditions and relative content difference is carried out the oranges and tangerines cultivar identification and quality evaluation has operability.
Chromatographic fingerprinting is the collection of illustrative plates of each component population characteristic of reflection test sample of obtaining of confession under directions sample herbal classic chromatographic technique, the chromatographic fingerprinting detection technique has become the effective means of internationally recognized control Chinese medicine and natural drug quality at present, now be widely used in the omnidistance quality monitoring of Chinese crude drug, plant extracts and traditional Chinese medicine, the chemical constitution chromatographic fingerprinting also is used for the quality monitoring to multiple agricultural product such as tealeaves, grape wine and tobaccos in addition, does not report as yet but this technology is applied to local oranges and tangerines improved seeds Study on Quality Control.
Summary of the invention
The object of the present invention is to provide the method for a kind of Fengjie navel orange authenticity and quality evaluation, this method can be set up the chromatographic fingerprinting of Fengjie navel orange fruit, and then can realize the evaluation of its true and false and the evaluation of quality.
The object of the present invention is achieved like this:
The method of a kind of Fengjie navel orange authenticity and quality evaluation is characterized in that, it carries out according to following steps:
(1) sampling: sample is respectively from least 10 producing regions in 20 small towns, navel orange geographical sign product protection district, Fengjie, in the maturity stage, selects that growth potential is medium, the trifoliate orange anvil of ecological condition and tree-like basically identical gives the garden 72-1 bud grafting plant stochastic sampling of growing up for five ages; Every sample selects 5 strains, and every strain mixes the back in-4 ℃ of low tempertaure storages from 5 pieces of the peripheral random acquisition ripening fruitss in tree crown middle part by place of production classification;
(2) need testing solution preparation: each 3 of all kinds of sample picked at random, strip pericarp and capsule lobe, 45 ℃ dry to constant weight, and materials respectively pericarp and pulp capsule lobe dry sample are pulverized 10min, cross 80 mesh sieves, get for the sample powder; Accurately take by weighing 0.2g sample powder, after adding methyl alcohol 20mL, the ultrasonic processing of 240W 30min, add the ultrasonic processing of 20mL methyl alcohol 240W 30min again after collecting supernatant, merge supernatant, in the centrifugal 10min of 5000r/min, decompression and solvent recovery, methanol constant volume is to 10mL, and 0.22 μ m filtering with microporous membrane gets need testing solution;
(3) HPLC analyzes: adopt the accurate 10 μ L need testing solution auto injections of drawing of Tianjin, island SIL-20A automatic sampler, the back adopts Tianjin, island LC-20AD type HPLC instrument to detect, chromatographic condition is as follows: chromatographic column is enlightening horse Diamonsil C18 post, and specification is 250mm * 4.6mm, 5 μ m; Flowing is acetonitrile-0.2% acetic acid aqueous solution flow system mutually; Flow velocity 1mLmin
-1, DAD detecting device 290nm detects, and be 35min detection time, and column oven is 30 ℃; Utilize the included angle cosine method to carry out similarity calculating between each chromatogram, utilizing state-promulgated pharmacopoeia committee chromatographic fingerprints of Chinese materia medica similarity evaluation software that need testing solution HPLC is detected data simultaneously and derive AIA(*.cdf through the LC-solution chromatographic work station) formatted file has the composition peak match, the contrast spectrogram generates and similarity is calculated, and sets up the flavones ingredient common characteristic HPLC chromatographic fingerprinting in Fengjie navel orange pericarp and the pulp respectively;
(4) authenticity and quality evaluation: be reference with the Fengjie navel orange pericarp that made up and the flavones ingredient common characteristic HPLC chromatographic fingerprinting in the pulp, randomly draw the navel orange of commercially available kind, utilize the HPLC detection method described in the need testing solution preparation method described in the step (2) and the step (3), the navel orange of the commercially available kind that processing is randomly drawed, and the chromatographic data of its pericarp and pulp carried out similarity analysis, can realize that the navel orange of this being randomly drawed commercially available kind carries out authenticity and quality evaluation.
In order better to realize the present invention, above-mentioned acetonitrile-0.2% acetic acid aqueous solution flow phase system adopts linear gradient elution: 0～5min:15%～25% acetonitrile; 5～10min:25%～35% acetonitrile; 10～20min:35%～30% acetonitrile; 20～30min:30%～25% acetonitrile; 30～38min:25%～15% acetonitrile; 38～40min:15%-15% acetonitrile.
The present invention has following beneficial effect:
The present invention is feature according to composition and the relative content of the characteristic active component flavones in the navel orange fruit of Fengjie, the Fengjie navel orange is done as a whole treating, made up Fengjie navel orange flavonoids characteristic chemical constituent chromatographic fingerprinting, and as with reference to having realized commercially available navel orange kind is carried out quality testing, monitoring and quality evaluation, be conducive to the Fengjie navel orange fruit true and false, the evaluation of the place of production and quality and control, overcome only with the producing region, fruit shape index and mouthfeel feature are identified the deficiency of poor accuracy, and the problem that mixes for solution China local high-quality sweet orange variety and quality provides reliable basis.In addition, the inventive method also has sample and prepares easyly, and chromatographic condition realizes easily, and stability and reappearance be advantage such as better all.
Description of drawings
Fig. 1-a is the Fengjie navel orange pericarp flavonoids characteristic chemical constituent collection of illustrative plates coupling in the embodiment of the invention 1, and it is S1 (PDA-Ch1) .CDF with reference to template.
Fig. 1-b is the Fengjie navel orange pericarp flavonoids characteristic chemical constituent chromatographic fingerprinting in the embodiment of the invention 1.
Fig. 2-a is the Fengjie navel orange pulp flavonoids characteristic chemical constituent collection of illustrative plates coupling in the embodiment of the invention 1, and it is S1 (PDA-Ch1) .CDF with reference to template.
Fig. 2-b is the Fengjie navel orange pulp flavonoids characteristic chemical constituent chromatographic fingerprinting in the embodiment of the invention 1.
Embodiment
Specifically describe below by the present invention of embodiment; but following examples only are used for the present invention is further detailed; can not be interpreted as the restriction to its protection domain, the person skilled in the art in this field can make some nonessential improvement and adjustment to the present invention according to foregoing.
Embodiment 1
The method of a kind of Fengjie navel orange authenticity and quality evaluation, it carries out according to following steps:
(1) sampling: sample is respectively from 14 different small towns, navel orange geographical sign product protection district, Fengjie, respectively to its numbering: S1: Shi Gang town, S2: Da Shu town, S3: An Ping township, S4: Baidicheng administration commission, S5: 19 groups in Taishan village, fair town, S6: originate in 4 groups in Taishan village, fair town, S7: originate in fair town siron village 4 societies, S8: 5 groups in Taishan village, fair town, S9: Hong Shi village, fair town, S10: health and happiness town, S11: Bai Di town, S12: five Ma Xiang, S13: Hefeng township, S14: eternally happy town; In the maturity stage, select that growth potential is medium, the trifoliate orange anvil of ecological condition and tree-like basically identical gives the garden 72-1 bud grafting plant stochastic sampling of growing up for five ages; Every sample selects 5 strains, and every strain mixes the back in-4 ℃ of low tempertaure storages from 5 pieces of the peripheral random acquisition ripening fruitss in tree crown middle part by place of production classification.
(2) need testing solution preparation: each 3 of all kinds of sample picked at random, strip pericarp and capsule lobe, 45 ℃ dry to constant weight, and materials respectively pericarp and capsule lobe dry sample are pulverized 10min, cross 80 mesh sieves, get for the sample powder; Accurately take by weighing 0.2g sample powder in the 150ml triangular flask, after adding methyl alcohol 20mL, the ultrasonic processing of 240W 30min, supernatant changes the 50ml centrifuge tube over to, adds the ultrasonic processing of 20mL methyl alcohol 240W 30min again, merge supernatant, in the centrifugal 10min of 5000r/min, decompression and solvent recovery, methanol constant volume is to 10mL, 0.22 μ m filtering with microporous membrane gets need testing solution.
(3) HPLC analyzes: adopt the accurate 10 μ L need testing solution auto injections of drawing of Tianjin, island SIL-20A automatic sampler, the back adopts Tianjin, island LC-20AD type HPLC instrument to detect, chromatographic condition is as follows: chromatographic column is enlightening horse Diamonsil C18 post (250mm * 4.6mm, 5 μ m); Flowing is acetonitrile-0.2% acetic acid aqueous solution flow system mutually, and adopts linear gradient elution: 0～5min:15%～25% acetonitrile; 5～10min:25%～35% acetonitrile; 10～20min:35%～30% acetonitrile; 20～30min:30%～25% acetonitrile; 30～38min:25%～15% acetonitrile; 38～40min:15%-15% acetonitrile; Flow velocity 1mLmin
-1, DAD detecting device 290nm detects, and be 35min detection time, and column oven is 30 ℃; Utilize the included angle cosine method to carry out similarity calculating between each chromatogram, utilizing state-promulgated pharmacopoeia committee chromatographic fingerprints of Chinese materia medica similarity evaluation (A version in 2004) software that need testing solution HPLC is detected data simultaneously and derive AIA(*.cdf through the LC-solution chromatographic work station) formatted file has the composition peak match, the contrast spectrogram generates and similarity is calculated, and sets up the flavones ingredient common characteristic HPLC chromatographic fingerprinting in Fengjie navel orange pericarp and the pulp respectively.
Make up HPLC flavonoids characteristic chemical constituent chromatographic fingerprinting and analyze comparison by pericarp and pulp sample to 14 batches of Fengjie navel oranges, the result shows: 14 batches of Fengjie navel orange sample pericarps become swarming to be evenly distributed with pulp extractive of general flavone chromatogram, degree of separation is higher, and separating effect is better.Utilize mean value method coupling Fengjie navel orange pericarp to have into swarming with pulp and be 35, the community coupling all can be realized in the principal ingredient peak, and matching result is good.Fengjie navel orange pericarp becomes for No. 26 swarming (RT=13.494) and pulp to become swarming (RT=13.476) area maximum for No. 23, and the reference that can be used as the relative peak area calculating of total peak becomes swarming.The retention time t at the total peak of 35 characteristics of Fengjie navel orange pericarp
RBe respectively 1.914min, 2.898min, 3.877min, 4.034min, 4.334min, 4.677min, 4.926min, 5.554min, 7.234min, 7.805min, 8.157min, 8.355min, 8.840min, 9.170min, 10.087min, 10.656min, 10.934min, 11.246min, 11.438min, 11.589min, 11.905min, 12.070min, 12.405min, 12.783min, 13.230min25Naringin, 13.494min26 (S) Hesperidin, 13.803min, 14.439min, 14.831min, 15.071min, 15.849min, 16.491min, 16.818min, 17.096min, 18.514min (see accompanying drawing 1-a, accompanying drawing 1-b, and following table 1), they have constituted Fengjie navel orange pericarp flavonoids characteristic chemical constituent finger-print; The retention time t at the total peak of 35 characteristics of Fengjie navel orange pulp
RBe respectively 2.898min, 3.905min, 4.035min, 4.355min, 4.655min, 5.428min, 6.168min, 7.757min, 8.327min, 8.818min, 9.096min, 9.368min, 9.91min, 10.08min, 10.24min, 10.548min, 11.074min, 11.225min, 11.417min, 11.885min, 12.386min, 12.769min, 13.476min23 (S) Hesperidin, 13.794min, 14.116min, 14.441min, 14.818min, 15.05min, 15.257min, 15.633min, 16.469min, 16.783min, 17.058min, 17.538min, 18.498min(following accompanying drawing 2-a, accompanying drawing 2-b, and following table 3), they have constituted Fengjie navel orange pulp flavonoids characteristic chemical constituent finger-print.The Fengjie navel orange sample pericarp that generates and pulp flavonoids characteristic chemical constituent chromatographic fingerprinting, can demonstrate fully composition compositing characteristic and the content distribution characteristic of chromocor extract in each sample fruit, have typicalness and specificity that Fengjie navel orange flavones ingredient is formed, it is marked as the retention time of swarming and relative content data and sees Table 1 and table 3.
Table 1 Fengjie navel orange pericarp flavonoids characteristic chemical constituent chromatographic fingerprinting relative peak area information table (becoming swarming with aurantiamarin is reference)
Table 3 Fengjie navel orange pulp flavonoids characteristic chemical constituent chromatographic fingerprinting relative peak area information table (becoming swarming with aurantiamarin is reference)
It is qualitative relatively to reach one point external standard method by absorption curve, determines that the ownership at navel orange pericarp flavonoids characteristic chemical constituent principal ingredient peak, Fengjie is as follows: t
RThe one-tenth swarming of=13.230min is aurantiin (Naringin), t
RThe one-tenth swarming of=13.494min (S) is aurantiamarin (Hesperidin), and the ownership at navel orange pulp flavonoids characteristic chemical constituent principal ingredient peak, Fengjie is as follows: t
RThe one-tenth swarming of=13.476min23 (S) is aurantiamarin (Hesperidin).
For examination Fengjie each sample room similarity analysis result of navel orange: the 14 batches of Fengjie navel orange sample pericarps that calculate by the included angle cosine method and the contrast collection of illustrative plates similarity of pulp flavonoids characteristic chemical constituent HPLC chromatographic fingerprinting and generation are respectively more than (table 2) more than 0.990 and 0.992 (table 4), illustrate that the contrast spectrogram is better representative, composition and the content characteristics that can fully represent Fengjie navel orange sample pericarp and pulp flavonoids characteristic chemical constituent can be utilized pericarp and pulp contrast spectral data that the Fengjie navel orange is carried out specificity and identify.Simultaneously the similarity between each sample HPLC chromatogram also higher (pericarp〉0.981, pulp〉0.973), illustrate that Fengjie navel orange sample pericarp flavonoids characteristic chemical constituent is formed and content has consistance highly.
Similarity between the navel orange sample pericarp flavonoids characteristic chemical constituent chromatographic fingerprinting of table 2 Fengjie
Similarity between the navel orange sample pulp flavonoids characteristic chemical constituent chromatographic fingerprinting of table 4 Fengjie
(4) authenticity and quality evaluation: the navel orange of randomly drawing commercially available kind, obtain each 10 pieces in commercially available crystal sugar orange, Fengjie Niu Heer navel orange, south, commercially available Jiangxi navel orange, commercially available Niu Heer navel orange, the orange fresh ripening fruits samples of northern a word used in place name 447 brocades ,-4 ℃ of low tempertaure storages are standby; According to the need testing solution preparation method of step (2) and the HPLC detection method in the step (3), handle the navel orange of the commercially available kind of randomly drawing afterwards; Be reference with the Fengjie navel orange pericarp that made up and the flavones ingredient common characteristic HPLC chromatographic fingerprinting in the pulp, carry out similarity analysis with the pericarp of the navel orange of randomly drawing commercially available kind and the chromatographic data of pulp, the result shows: Fengjie navel orange, Niu Heer navel orange and south, Jiangxi navel orange all belong to the bud mutation strain system of Washington navel, and sibship is nearer; Crystal sugar orange belongs to low sour-sweet orange, and northern a word used in place name 447 bright and beautiful oranges then belong to common sweet orange, and these two materials and Washington navel sibship are far away.
Be reference with the Fengjie navel orange pericarp and the pulp flavonoids characteristic chemical constituent standard colour chart finger-print that have made up, adopt the included angle cosine method that Fengjie product Niu Heer navel orange and other four kinds commercially available sweet orange fruit flavone extract chromatograms are carried out similarity calculating, the result shows: Niu Heer navel orange and commercially available Niu Heer navel orange sample pericarp are produced in the Fengjie, pulp chromatogram and Fengjie navel orange similarity are higher, similarity (sees the following form 5) about 0.9, but 0.990 similarity between Da Fengjie navel orange sample chromatogram figure does not illustrate that composition and the content of the nearly edge sweet orange kind flavones ingredient under the different home environment conditions has notable difference.And the overall similarity of commercially available crystal sugar orange and northern a word used in place name 447 brocade oranges and Fengjie navel orange fruit pulp flavonoids characteristic chemical constituent is between 0.6～0.8, south, commercially available Jiangxi navel orange is minimum with Fengjie navel orange similarity, shows that Fengjie navel orange pericarp and pulp flavonoids characteristic chemical constituent are formed and content characteristics has and carries out the using value that puppet is emitted the fruit evaluation.
Table 5 Fengjie navel orange standard flavonoids characteristic and branch chromatogram finger-print and different oranges and tangerines sample similarity
Claims (2)
1. the method for a Fengjie navel orange authenticity and quality evaluation is characterized in that it carries out according to following steps:
(1) sampling: sample is respectively from least 10 producing regions in 20 small towns, navel orange geographical sign product protection district, Fengjie, in the maturity stage, selects that growth potential is medium, the trifoliate orange anvil of ecological condition and tree-like basically identical gives the garden 72-1 bud grafting plant stochastic sampling of growing up for five ages; Every sample selects 5 strains, and every strain mixes the back in-4 ℃ of low tempertaure storages from 5 pieces of the peripheral random acquisition ripening fruitss in tree crown middle part by place of production classification;
(2) need testing solution preparation: each 3 of all kinds of sample picked at random, strip pericarp and capsule lobe, 45 ℃ dry to constant weight, and materials respectively pericarp and pulp capsule lobe dry sample are pulverized 10 min, cross 80 mesh sieves, get for the sample powder; Accurately take by weighing 0.2 g sample powder, after adding methyl alcohol 20 mL, ultrasonic processing 30 min of 240 W, add ultrasonic processing 30 min of 20 mL methyl alcohol, 240 W again after collecting supernatant, merge supernatant, in centrifugal 10 min of 5000 r/min, decompression and solvent recovery, methanol constant volume to 10 mL, 0.22 μ m filtering with microporous membrane gets need testing solution;
(3) HPLC analyzes: adopt the accurate 10 μ L need testing solution auto injections of drawing of Tianjin, island SIL-20A automatic sampler, the back adopts Tianjin, island LC-20AD type HPLC instrument to detect, chromatographic condition is as follows: chromatographic column is enlightening horse Diamonsil C18 post, and specification is 250 mm * 4.6 mm, 5 μ m; Flowing is acetonitrile-0.2% acetic acid aqueous solution flow system mutually; Flow velocity 1 mLmin
-1, DAD detecting device 290 nm detect, and be 35 min detection time, and column oven is 30 ℃; Utilize the included angle cosine method to carry out similarity calculating between each chromatogram, utilizing state-promulgated pharmacopoeia committee chromatographic fingerprints of Chinese materia medica similarity evaluation software that need testing solution HPLC is detected data simultaneously and derive AIA(*.cdf through the LC-solution chromatographic work station) formatted file has the composition peak match, the contrast spectrogram generates and similarity is calculated, and sets up the flavones ingredient common characteristic HPLC chromatographic fingerprinting in Fengjie navel orange pericarp and the pulp respectively;
(4) authenticity and quality evaluation: be reference with the Fengjie navel orange pericarp that made up and the flavones ingredient common characteristic HPLC chromatographic fingerprinting in the pulp, randomly draw the navel orange of commercially available kind, utilize the HPLC detection method described in the need testing solution preparation method described in the step (2) and the step (3), the navel orange of the commercially available kind that processing is randomly drawed, and the chromatographic data of its pericarp and pulp carried out similarity analysis, can realize that the navel orange of this being randomly drawed commercially available kind carries out authenticity and quality evaluation.
2. the method for Fengjie navel orange authenticity according to claim 1 and quality evaluation is characterized in that: described acetonitrile-0.2% acetic acid aqueous solution flow phase system employing linear gradient elution: 0～5min:15%～25% acetonitrile; 5～10 min:25%～35% acetonitrile; 10～20 min:35%～30% acetonitrile; 20～30 min:30%～25% acetonitrile; 30～38 min:25%～15% acetonitrile; 38～40 min:15%-15% acetonitriles.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2013102961174A CN103344729A (en) | 2013-07-15 | 2013-07-15 | Method for identifying authenticity and evaluating quality of Fengjie navel oranges |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2013102961174A CN103344729A (en) | 2013-07-15 | 2013-07-15 | Method for identifying authenticity and evaluating quality of Fengjie navel oranges |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN103344729A true CN103344729A (en) | 2013-10-09 |
Family
ID=49279543
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2013102961174A Pending CN103344729A (en) | 2013-07-15 | 2013-07-15 | Method for identifying authenticity and evaluating quality of Fengjie navel oranges |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN103344729A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104122354A (en) * | 2014-06-17 | 2014-10-29 | 山东绿原农业科技研究院 | Method for identifying authenticity and pesticide residue content of fruits and vegetables by use of HPLC (High Performance Liquid Chromatography) fingerprint spectrum |
| CN104316635A (en) * | 2014-10-28 | 2015-01-28 | 石河子大学 | Method for rapidly identifying flavor and quality of fruits |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH0948969A (en) * | 1995-08-04 | 1997-02-18 | Pokka Corp | Antioxidizing substance derived from citrus fruit |
| CN1959407A (en) * | 2006-10-20 | 2007-05-09 | 中山大学 | Method for constructing fingerprint HPLC of Shatian pomelo, and normal fingerprint |
| CN101178389A (en) * | 2007-12-05 | 2008-05-14 | 广东省农业科学院农业生物技术研究所 | Method for identifying genuine-fake of black rice or produce therefrom by HPLC fingerprint |
| CN102011196A (en) * | 2010-10-21 | 2011-04-13 | 中国农业科学院柑桔研究所 | Citrus breed standard DNA fingerprint spectrum library and constructing method thereof |
-
2013
- 2013-07-15 CN CN2013102961174A patent/CN103344729A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH0948969A (en) * | 1995-08-04 | 1997-02-18 | Pokka Corp | Antioxidizing substance derived from citrus fruit |
| CN1959407A (en) * | 2006-10-20 | 2007-05-09 | 中山大学 | Method for constructing fingerprint HPLC of Shatian pomelo, and normal fingerprint |
| CN101178389A (en) * | 2007-12-05 | 2008-05-14 | 广东省农业科学院农业生物技术研究所 | Method for identifying genuine-fake of black rice or produce therefrom by HPLC fingerprint |
| CN102011196A (en) * | 2010-10-21 | 2011-04-13 | 中国农业科学院柑桔研究所 | Citrus breed standard DNA fingerprint spectrum library and constructing method thereof |
Non-Patent Citations (8)
| Title |
|---|
| JIA ZHANG ET AL.: "Review of the current application of fingerprinting allowing detection of food adulteration and fraud in China", 《FOOD CONTROL》 * |
| JIA-YU ZHANG ET AL.: "Characterization of Polymethoxylated Flavonoids (PMFs) in the Peels of ‘Shatangju’ Mandarin (Citrus reticulata Blanco) by Online High-Performance Liquid Chromatography Coupled to Photodiode Array Detection and Electrospray Tandem Mass Spectrometry", 《J. AGRIC. FOOD CHEM.》 * |
| 付娜: "奉节脐橙指纹图谱研究", 《万方数据库》 * |
| 刘小华等: "脐橙功效成分提取与质量检测研究进展", 《食品研究与开发》 * |
| 张元梅等: "高效液相色谱法同时测定柑橘果实中18种类黄酮的含量", 《中国农业科学》 * |
| 张泽煌 等: "黄皮果实成熟和低温贮藏过程中有机酸的代谢", 《福建农业学报》 * |
| 罗安才 等: "柑桔果实糖酸比及线粒体乌头酸酶活性的变化", 《西南农业大学学报》 * |
| 陈颖 等: "现代食品真伪检测鉴别技术", 《食品与发酵工业》 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104122354A (en) * | 2014-06-17 | 2014-10-29 | 山东绿原农业科技研究院 | Method for identifying authenticity and pesticide residue content of fruits and vegetables by use of HPLC (High Performance Liquid Chromatography) fingerprint spectrum |
| CN104122354B (en) * | 2014-06-17 | 2016-01-20 | 山东绿原农业科技研究院 | A kind of method utilizing the HPLC fingerprint identification fruits and vegetables true and false |
| CN104316635A (en) * | 2014-10-28 | 2015-01-28 | 石河子大学 | Method for rapidly identifying flavor and quality of fruits |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN107246892B (en) | A kind of quality synthesis measuring method for eating Chinese wolfberry fruit raw | |
| CN102749348B (en) | Method for identifying active components in medicinal plant | |
| Zhao et al. | Fruit flavonoid variation between and within four cultivated Citrus species evaluated by UPLC-PDA system | |
| Song et al. | Chemical comparison and classification of Radix Astragali by determination of isoflavonoids and astragalosides | |
| CN105353053B (en) | The content assaying method of scutellarin and scutellarin in a kind of Sculellaria barbata medicinal material or its granule | |
| CN108226313A (en) | In glutinous rehmannia while methods of glycosides measure and fingerprint map construction method | |
| CN109633012B (en) | Identification method of Zhejiang ophiopogon root | |
| Ding et al. | Simultaneous quantification of eleven bioactive components of male flowers of Eucommia ulmoides oliver by HPLC and their quality evaluation by chemical fingerprint analysis with hierarchical clustering analysis | |
| Lee et al. | Quantification of isoflavonoids and triterpene saponins in Astragali Radix, the root of Astragalus membranaceus, via reverse-phase high-performance liquid chromatography coupled with integrated pulsed amperometric detection | |
| CN110297060B (en) | Fingerprint detection method and fingerprint thereof for ixeris sonchifolia medicinal materials | |
| Tan et al. | Localization and dynamic change of saikosaponin in root of Bupleurum chinense | |
| CN108267535B (en) | Green tangerine peel medicinal material fingerprint detection method and fingerprint thereof | |
| CN103344729A (en) | Method for identifying authenticity and evaluating quality of Fengjie navel oranges | |
| CN103869019A (en) | High performance liquid chromatography (HPLC) fingerprint establishment method of Fenggang zinc-selenium tea | |
| CN101327246A (en) | Radix astragali medicinal materials, intermediate body and method for testing fingerprint of formulation thereof as well as standard fingerprint | |
| CN103868943B (en) | A kind of method of differentiating Fructus Ligustri Lucidi or spin-off | |
| CN109828053A (en) | The raw HERBA DENDROBII of a kind of pair of stone and the method for setting raw HERBA DENDROBII progress chromatographic identification | |
| CN105181838A (en) | Method for establishing HPLC fingerprint of Liubanhong garlic enzymatic hydrolysis product | |
| CN105158369A (en) | Establishment method of HPLC (High Performance Liquid Chromatography) fingerprint spectrum of allinase inactivated extract of six-peal red garlics | |
| CN110823828A (en) | Method for identifying Wumai green wormwood in different producing areas | |
| CN105158391B (en) | Callus induction cultivates the method for building up of Garlic enzyme-deactivating extractive HPLC fingerprint | |
| CN104458954B (en) | A kind of dodder formulation granule finger printing and method for building up thereof | |
| CN109633033B (en) | Characteristic map method for identifying bitter orange in river | |
| CN102419350B (en) | Method for carrying out simultaneous quantitative analysis on four lignan components in Chinese magnoliavine raw material and Chinese magnoliavine extract | |
| CN106442809A (en) | Sphenomeris chinensis fingerprinting construction method and Sphenomeris chinensis fingerprinting |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C12 | Rejection of a patent application after its publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20131009 |