CN103344639B - The detector tube of pyrroles's adduct in a kind of quick detection urine - Google Patents
The detector tube of pyrroles's adduct in a kind of quick detection urine Download PDFInfo
- Publication number
- CN103344639B CN103344639B CN201310291887.XA CN201310291887A CN103344639B CN 103344639 B CN103344639 B CN 103344639B CN 201310291887 A CN201310291887 A CN 201310291887A CN 103344639 B CN103344639 B CN 103344639B
- Authority
- CN
- China
- Prior art keywords
- adduct
- pyrroles
- developer
- urine
- detector tube
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Abstract
The invention discloses the detector tube of pyrroles's adduct in a kind of quick detection urine, comprise the body of two ends sealing, one end of described body is butt end, and the other end is elongated end, and butt end length is 3cm, and internal diameter is 1cm, and elongated end length is 3mm, and internal diameter is 0.2cm.Butt end inwall scribbles developer (dimethylaminobenzaldehyde).The method utilizing the detector tube of pyrroles's adduct in above-mentioned quick detection urine to detect pyrroles's adduct in urine is: sealing place of cutting elongated end with scissors, butt end is extruded with hand, detected sample is sucked (soakage is 1.0ml at least) in body, then chromoscope is inverted jolting for several times, sample and developer are fully reacted, after 1 minute, the color produce sample and contrast ratio colour atla compare, to determine the concentration of pyrroles's adduct in sample.According to the content of pyrroles's adduct in urine, early warning and instruction workman normal hexane neurotrosis degree.
Description
Technical field
The present invention relates to the detector tube of pyrroles's adduct in a kind of quick detection urine.
Background technology
After worker exposure's normal hexane, normal hexane is converted into 2 in liver, 5-acetyl butyryl, acetyl butyryl is combined with amino residue further and is formed containing 2, the adduct (pyrroles's adduct) of 5-dimethyl pyrrole, pyrroles's adduct excretes along with urine, therefore, can be reflected the Exposure of normal hexane by the concentration detecting pyrroles's adduct in urine.At present, there is no a kind of product and the method that can detect the concentration of pyrroles's adduct quickly and easily.
Summary of the invention
For above-mentioned prior art, the present invention reacts the principle that can form mauve color according to paradime thylaminobenzaldehyde and pyrrole ring, devises the detector tube of the concentration of pyrroles's adduct in a kind of quick detection urine.
The present invention is achieved by the following technical solutions:
In quick detection urine, a detector tube for pyrroles's adduct, comprises the body of two ends sealing, body one end inwall scribbles developer (dimethylaminobenzaldehyde).
Further, described body is polyethylene pipe.
Further, one end of described body is butt end, and the other end is elongated end, and developer is coated on the inwall of butt end.
Further, the butt end length of described body is 3cm, and internal diameter is 1cm; Elongated end length is 3mm, and internal diameter is 0.2cm.
Further, the method of described coating developer is: be 3%(unit g/ml by 0.2ml mass body volume concentrations) dimethylaminobenzaldehyde solution add in the butt end of detector tube, at ambient temperature, chromoscope is inverted (making butt end down), until developer volatilization is dry; After treating developer volatilization, elongated end seals in the mode of heating and melting, to obtain final product; Wherein, the consisting of of solvent of dimethylaminobenzaldehyde solution: the ethanol of 14% boron trifluoride methanol solution (mass body volume concentrations, unit the g/ml)+40ml of 60ml.
The method utilizing the detector tube of pyrroles's adduct in above-mentioned quick detection urine to detect pyrroles's adduct in urine is: sealing place of cutting elongated end with scissors, butt end is extruded with hand, detected sample is sucked (soakage is 1.0ml at least) in body, then chromoscope is inverted (making butt end down) jolting for several times, sample and developer are fully reacted, after 1 minute, the color produce sample and contrast ratio colour atla compare, to determine the concentration of pyrroles's adduct in sample.According to the content of pyrroles's adduct in urine, early warning and instruction workman normal hexane neurotrosis degree.
Described contrast ratio colour atla prepares by the following method: first prepare 2, the normal gradients strength solution of 5-dimethyl pyrrole, then the dimethylaminobenzaldehyde solution of developer (3%(unit g/ml) is added, 14% boron trifluoride methanol solution (the mass body volume concentrations of consisting of of solvent: 60ml, unit g/ml) ethanol of+40ml) reaction, react after 1 minute, observe the color of solution of variable concentrations, and according to each group gradient concentration and corresponding color as standard control color.
Points for attention: (1), due to namely ineffective after developer reaction, therefore, every root detector tube can only use once, can not reuse; (2) experiment proves that this detector tube retention cycle can maintain more than 6 months, not impact effect; (3) this detector tube is mainly for the detection of pyrroles's adduct in normal hexane workers exposed urine, should get rid of the abnormal false positive results caused of workman's urobilinogen.
Accompanying drawing explanation
Fig. 1 is the structural representation of the detector tube of pyrroles's adduct in quick detection urine of the present invention.
Wherein, 1, body; 2, butt end; 3, elongated end; 4, developer.
Fig. 2 is standard color comparison card (× 1000nmol/ml) schematic diagram.
Embodiment
Below in conjunction with embodiment, the present invention is further illustrated.
The detector tube of pyrroles's adduct in a kind of quick detection urine, the body 1(polythene material comprising two ends sealing is made, be easy to extrude pipette samples), as shown in Figure 1, one end of described body 1 is butt end 2, and the other end is elongated end 3, butt end 2 length is the sample that 3cm(can hold 2.5ml), internal diameter is 1cm, and elongated end 3 length is 3mm, and internal diameter is 0.2cm.Butt end 2 inwall scribbles developer 4(dimethylaminobenzaldehyde).
The method of described coating developer is: be 3%(unit g/ml by 0.2ml mass body volume concentrations) dimethylaminobenzaldehyde solution add in the butt end of detector tube, at ambient temperature, chromoscope is inverted (making butt end down), until developer volatilization is dry; After treating developer volatilization, elongated end seals in the mode of heating and melting, to obtain final product; Wherein, the consisting of of solvent of dimethylaminobenzaldehyde solution: the ethanol of 14% boron trifluoride methanol solution (mass body volume concentrations, unit the g/ml)+40ml of 60ml.
The method utilizing the detector tube of pyrroles's adduct in above-mentioned quick detection urine to detect pyrroles's adduct in urine is: sealing place of cutting elongated end with scissors, butt end is extruded with hand, detected sample is sucked (soakage is 1.0ml at least) in body, then chromoscope is inverted (making butt end down) jolting for several times, sample and developer are fully reacted, after 1 minute, the color produce sample and contrast ratio colour atla compare, to determine the concentration of pyrroles's adduct in sample.According to the content of pyrroles's adduct in urine, early warning and instruction workman normal hexane neurotrosis degree.
Described contrast ratio colour atla prepares by the following method: the normal gradients strength solution (0.06mmol/L ~ 15mmol/L) first preparing pyrroles's adduct, get 2.5ml and be placed in test tube, add 0.2ml developer (the dimethylaminobenzaldehyde solution of 3%, the ethanol of the 14% boron trifluoride methanol solution+40ml of consisting of of solvent: 60ml) reaction, react after 1 minute, observe the color of solution of variable concentrations, and according to each group gradient concentration and corresponding color as standard control color.
Points for attention: (1), due to namely ineffective after developer reaction, therefore, every root detector tube can only use once, can not reuse; (2) experiment proves that this detector tube retention cycle can maintain more than 6 months, not impact effect; (3) this detector tube is mainly for the detection of pyrroles's adduct in normal hexane workers exposed urine, should get rid of the abnormal false positive results caused of workman's urobilinogen.
Claims (5)
1. one kind is detected the detector tube of pyrroles's adduct in urine fast, it is characterized in that: the body comprising two ends sealing, one end of described body is butt end, the other end is elongated end, and described body is made up of polythene material, and developer is coated on the inwall of butt end, developer is dimethylaminobenzaldehyde, the coating process of described developer is: by mass body volume concentrations be 3% dimethylaminobenzaldehyde solution add in the butt end of detector tube, at ambient temperature, developer is volatilized dry; After treating developer volatilization, elongated end seals in the mode of heating and melting, to obtain final product; Wherein, the consisting of of solvent of dimethylaminobenzaldehyde solution: 60ml mass body volume concentrations is the ethanol of 14% boron trifluoride methanol solution+40ml; The concentration unit of wherein said quality volume is g/mL.
2., according to the detector tube of pyrroles's adduct in a kind of quick detection urine described in claim 1, it is characterized in that: the butt end length of described body is 3cm, and internal diameter is 1cm; Elongated end length is 3mm, and internal diameter is 0.2cm.
3. utilize the detector tube of pyrroles's adduct in a kind of quick detection urine described in claim 1 or 2 to detect the method for pyrroles's adduct in urine, it is characterized in that: sealing place of cutting elongated end, butt end is extruded with hand, detected sample is sucked in body, soakage is 1.0ml at least, then being inverted by chromoscope makes butt end down, jolting for several times, sample and developer are fully reacted, after 1 minute, the color produce sample and contrast ratio colour atla compare, thus determine the concentration of pyrroles's adduct in sample.
4. method according to claim 3, is characterized in that: according to the content of pyrroles's adduct in urine, early warning and instruction workman normal hexane neurotrosis degree.
5. according to the method described in claim 4, it is characterized in that: described contrast ratio colour atla prepares by the following method: first prepare 2, the normal gradients strength solution of 5-dimethyl pyrrole, then developer reaction is added, react after 1 minute, observe the color of the solution of variable concentrations, and according to each group gradient concentration and corresponding color production standard contrast color.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310291887.XA CN103344639B (en) | 2013-07-11 | 2013-07-11 | The detector tube of pyrroles's adduct in a kind of quick detection urine |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310291887.XA CN103344639B (en) | 2013-07-11 | 2013-07-11 | The detector tube of pyrroles's adduct in a kind of quick detection urine |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN103344639A CN103344639A (en) | 2013-10-09 |
| CN103344639B true CN103344639B (en) | 2015-10-28 |
Family
ID=49279453
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201310291887.XA Expired - Fee Related CN103344639B (en) | 2013-07-11 | 2013-07-11 | The detector tube of pyrroles's adduct in a kind of quick detection urine |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN103344639B (en) |
Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0393334A2 (en) * | 1989-04-19 | 1990-10-24 | Robert Aaron Levine | Determination of lymphocyte reactivity to specific antigens in blood |
| US5653941A (en) * | 1996-07-29 | 1997-08-05 | Veretto; Bobby | Food spoilage detector |
| CN1491274A (en) * | 2001-02-27 | 2004-04-21 | P&G | Method for removing polymer films in manufacture of aqueous solutions containing acrylates copolymer |
| CN101029874A (en) * | 2006-11-30 | 2007-09-05 | 白莉 | Liquid for testing indigo substrate and its testing tube |
| CN201319020Y (en) * | 2008-12-12 | 2009-09-30 | 中国人民解放军军事医学科学院卫生学环境医学研究所 | An absorption-sampling water quality detecting tube |
| CN101203748B (en) * | 2005-05-20 | 2011-09-14 | 爱科来株式会社 | Protein-immobilized membrane, method for immobilization of protein, enzyme-immobilized electrode, and biosensor |
| CN102448732A (en) * | 2009-04-03 | 2012-05-09 | 日本制纸株式会社 | Coating solution for heat-sensitive color-developing layer, and heat-sensitive recording material |
-
2013
- 2013-07-11 CN CN201310291887.XA patent/CN103344639B/en not_active Expired - Fee Related
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0393334A2 (en) * | 1989-04-19 | 1990-10-24 | Robert Aaron Levine | Determination of lymphocyte reactivity to specific antigens in blood |
| US5653941A (en) * | 1996-07-29 | 1997-08-05 | Veretto; Bobby | Food spoilage detector |
| CN1491274A (en) * | 2001-02-27 | 2004-04-21 | P&G | Method for removing polymer films in manufacture of aqueous solutions containing acrylates copolymer |
| CN101203748B (en) * | 2005-05-20 | 2011-09-14 | 爱科来株式会社 | Protein-immobilized membrane, method for immobilization of protein, enzyme-immobilized electrode, and biosensor |
| CN101029874A (en) * | 2006-11-30 | 2007-09-05 | 白莉 | Liquid for testing indigo substrate and its testing tube |
| CN201319020Y (en) * | 2008-12-12 | 2009-09-30 | 中国人民解放军军事医学科学院卫生学环境医学研究所 | An absorption-sampling water quality detecting tube |
| CN102448732A (en) * | 2009-04-03 | 2012-05-09 | 日本制纸株式会社 | Coating solution for heat-sensitive color-developing layer, and heat-sensitive recording material |
Non-Patent Citations (1)
| Title |
|---|
| 夏曙华.实验七 尿胆原定性测定（改良Ehrlich法）.《临床基础检验学实验指导》.科学出版社,2012,第62-63页. * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN103344639A (en) | 2013-10-09 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN103399002B (en) | A kind of method for quick of trace dioxin | |
| He et al. | A low-cost and label-free assay for hydrazine using MnO2 nanosheets as colorimetric probes | |
| CN101551328B (en) | Resonance scattering spectrometry for rapidly measuring ammonia nitrogen in water | |
| CN108732218A (en) | A kind of electrochemical sensor measuring 2,4,6- trichlorophenol, 2,4,6,-Ts and its preparation and application | |
| CN103344594A (en) | Method for detecting ammonia emission in coal ash | |
| Huo et al. | MOF-based hybrid film for multiphase detection of sulfur dioxide with colorimetric and surface-enhanced Raman scattering readout | |
| CN104597258A (en) | Method for detecting 17beta-estradiol by employing colorimetric method based on nucleic acid aptamer | |
| Yin et al. | Determination of sulfite in water samples by flow injection analysis with fluorescence detection | |
| CN101936947B (en) | Calibration sample of ion mobility spectrometer as well as preparation method and use method thereof | |
| CN106248609B (en) | A kind of method that ultraviolet specrophotometer measures hexafluorophosphoric acid lithium content in lithium-ion battery electrolytes | |
| CN103344639B (en) | The detector tube of pyrroles's adduct in a kind of quick detection urine | |
| CN105181868A (en) | Method for simultaneous detection of 13 benzene compounds and phthalic acid ester compounds in water-borne adhesive and application thereof | |
| CN103091410B (en) | Method for measuring phthalate type compound content in reconstituted tobacco | |
| Hong et al. | A sensitive colorimetric hydrogen sulfide detection approach based on copper-metal–organic frameworks and a smartphone | |
| CN104568890A (en) | Method for detecting ethidium bromide by fluorescent carbon dot probe | |
| CN109632982A (en) | A kind of method of quick measurement natural estrogen combination | |
| CN106706611B (en) | Method for immobilizing indicator by using porous silica gel material, gas sensor and application | |
| CN109738250A (en) | The pre-treatment and analysis method of sulfide in solid waste | |
| CN102827933B (en) | Kit for qualitative detection of pinewood nematode and detection method thereof | |
| CN100487426C (en) | Boron determination solution and colorimetric determination tube therefor | |
| CN103822914B (en) | Test strip for detecting H2S/S＜2-＞ and method for detecting H2S/S＜2-＞ | |
| CN104034761B (en) | A kind of device and method detecting OBP and pheromone cohesive process | |
| CN103913524B (en) | The detection method of carbon disulphide and thiophene content in a kind of Simultaneously test coking benzene | |
| CN104568940B (en) | Method for detecting hydrogen sulfide gas based on nanocrystalline celluloses | |
| CN104535503A (en) | Method for preparing and using detection agent for measuring content of cobalt in water body |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20151028 Termination date: 20160711 |