CN103341191B - A kind of deactivation lysine strain and detection method - Google Patents
A kind of deactivation lysine strain and detection method Download PDFInfo
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- CN103341191B CN103341191B CN201310281282.2A CN201310281282A CN103341191B CN 103341191 B CN103341191 B CN 103341191B CN 201310281282 A CN201310281282 A CN 201310281282A CN 103341191 B CN103341191 B CN 103341191B
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Abstract
Deactivation lysine strain and detection method, first the pH value of fermentation liquid is regulated with concentrated sulphuric acid, then the fermentation liquid insulation regulated after PH is left standstill 30 minutes, dwell temperature is 60 degree, finally detect through slat chain conveyor again, it is characterized in that the concentration of described concentrated sulphuric acid is 98.5%, rank is AR, the pH value of described fermentation liquid is 2, the invention has the beneficial effects as follows deactivation lysine strain and detection method, namely the energy of high temperature sterilize has been saved, directly can carry out again the subsequent handling of fermenting, save time, raise the efficiency, add the fluency of technique, and there is not the bacterial strain of any survival in the fermentation liquid after deactivation, can guarantee ferment subsequent technique and sewage in the middle of exist without viable bacteria, decrease the probability that strain leaks, i.e. energy-conserving and environment-protective, turn improve the quality of product.
Description
Technical field
The invention belongs to biological technical field, a kind of method being more particularly deactivation lysine strain and deactivation result is detected.
Background technology
At present, produce in the process of lysine, the mode of production of general employing continuous fermentation, continuous fermentation training method needs to arrange holding vessel and is used for storing fermentation liquid and storing cultivating the fermentation liquid after terminating, and the non-seal container of the many uses of the holding vessel of prior art, this will cause in yeasting exists viable bacteria, easily cause microbiological contamination, infection phage and possible strain leak, Given this, at present, major part producer adopts the mode of high temperature sterilize to carry out deactivation, temperature generally adopts 121 degree, after cooling, the pH value carrying out fermentation liquid again regulates, this process is longer, not only consume the energy but also lose time, waste time and energy, and be unfavorable for the continuous seepage of lysine.
Summary of the invention
In order to solve the problem, the invention provides a kind of time saving and energy saving, energy savings, be conducive to lysine quantity-produced deactivation lysine strain and detection method, technical scheme of the present invention is: the pH value first regulating fermentation liquid with concentrated sulphuric acid, then the fermentation liquid insulation regulated after PH is left standstill 30 minutes, dwell temperature is 60 degree, finally detects through slat chain conveyor again, it is characterized in that the concentration of described concentrated sulphuric acid is 98.5%, rank is AR, and the pH value of described fermentation liquid is 2.
Slat chain conveyor of the present invention detects, it is characterized in that the fermentation liquid 50ml after getting above-mentioned insulation leaves standstill is placed in 250ml triangular flask, build stannum cap, be placed in thermostat water bath and be heated to 60 degree, be incubated after 30 minutes, take a morsel and use inoculating loop uniform application on plating medium, be placed in water isolation type electro-heating standing-temperature cultivator to cultivate, cultivation temperature 37 degree, incubation time 24 hours, then check the growing state of bacterial strain on plating medium, exist without viable bacteria.
The invention has the beneficial effects as follows deactivation lysine strain and detection method, namely the energy of high temperature sterilize has been saved, directly can carry out again the subsequent handling of fermenting, save time, raise the efficiency, add the fluency of technique, and there is not the bacterial strain of any survival in the fermentation liquid after deactivation, can guarantee ferment subsequent technique and sewage in the middle of without viable bacteria exist, decrease strain leakage probability, i.e. energy-conserving and environment-protective, turn improve the quality of product.
Detailed description of the invention
The present invention is described in further detail in conjunction with the embodiments, so that the public grasps implementation method of the present invention better, specific embodiment of the present invention is: deactivation lysine strain and detection method, first regulate the pH value of fermentation liquid with concentrated sulphuric acid, then the fermentation liquid insulation regulated after PH is left standstill 30 minutes, dwell temperature is 60 degree, finally detect through slat chain conveyor again, it is characterized in that the concentration of described concentrated sulphuric acid is 98.5%, rank is AR, and the pH value of described fermentation liquid is 2.
Slat chain conveyor of the present invention detects, it is characterized in that the fermentation liquid 50ml after getting above-mentioned insulation leaves standstill is placed in 250ml triangular flask, build stannum cap, be placed in thermostat water bath and be heated to 60 degree, be incubated after 30 minutes, take a morsel and use inoculating loop uniform application on plating medium, be placed in water isolation type electro-heating standing-temperature cultivator to cultivate, cultivation temperature 37 degree, incubation time 24 hours, then check the growing state of bacterial strain on plating medium, exist without viable bacteria.
The invention has the beneficial effects as follows deactivation lysine strain and detection method, namely the energy of high temperature sterilize has been saved, directly can carry out again the subsequent handling of fermenting, save time, raise the efficiency, add the fluency of technique, and there is not the bacterial strain of any survival in the fermentation liquid after deactivation, can guarantee ferment subsequent technique and sewage in the middle of without viable bacteria exist, decrease strain leakage probability, i.e. energy-conserving and environment-protective, turn improve the quality of product.
Claims (1)
1. a deactivation lysine strain and detection method, it is characterized in that the pH value first regulating fermentation liquid with concentrated sulphuric acid, then the fermentation liquid insulation regulated after PH is left standstill 30 minutes, dwell temperature is 60 degree, finally detect through slat chain conveyor again, the concentration of concentrated sulphuric acid is 98.5%, rank is AR, the pH value of fermentation liquid is 2, the fermentation liquid 50ml of gentle and quiet postpone of going bail for is placed in 250ml triangular flask, build stannum cap, be placed in thermostat water bath and be heated to 60 degree, be incubated after 30 minutes, take a morsel and use inoculating loop uniform application on plating medium, be placed in water isolation type electro-heating standing-temperature cultivator to cultivate, cultivation temperature 37 degree, cultivate 24 hours, then the growing state of bacterial strain on plating medium is checked.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310281282.2A CN103341191B (en) | 2013-07-05 | 2013-07-05 | A kind of deactivation lysine strain and detection method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310281282.2A CN103341191B (en) | 2013-07-05 | 2013-07-05 | A kind of deactivation lysine strain and detection method |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN103341191A CN103341191A (en) | 2013-10-09 |
| CN103341191B true CN103341191B (en) | 2015-11-18 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
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| CN201310281282.2A Expired - Fee Related CN103341191B (en) | 2013-07-05 | 2013-07-05 | A kind of deactivation lysine strain and detection method |
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Family Cites Families (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101456822B (en) * | 2008-11-28 | 2012-07-25 | 山东恩贝生物工程有限公司 | Novel process for extracting threonine |
| CN101580800B (en) * | 2009-05-27 | 2011-03-09 | 安徽丰原生物化学股份有限公司 | Separation method of lysine bacteria |
| US20140315789A1 (en) * | 2011-11-23 | 2014-10-23 | Newsouth Innovations Pty Limited | Antimicrobial peptides and uses thereof |
| CN103007308B (en) * | 2012-12-21 | 2014-05-28 | 菱花集团有限公司 | Material sterilization method integrating continuous sterilization and filled can sterilization in lysine fermentation process |
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2013
- 2013-07-05 CN CN201310281282.2A patent/CN103341191B/en not_active Expired - Fee Related
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| CN103341191A (en) | 2013-10-09 |
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