Detailed description of the invention
By the following examples the present invention is specifically described; be necessary to be pointed out that at this present embodiment is only used to further illustrate the present invention; can not be interpreted as limiting the scope of the invention, the person skilled in the art in this field can make some nonessential improvement and adjustment according to the content of the invention described above.
Effective ingredient of the present invention is guar gum hydrogel, polymethyl acrylate-chitosan nano complex, methyl salicylate, heparin sodium, allantoin, menthol, Camphora and Borneolum Syntheticum.Wherein:
Embodiment mono-
Under 25 DEG C of conditions, get 1mmol/L boric acid 1L, add 1mmol/L NaOH, while utilizing PH meter to regulate PH=11, stop adding in the process adding, react after 10 minutes, add guar gum 50g, 25 DEG C are reacted and obtain guar gum hydrogel half an hour.
20g chitosan, 10g acrylic acid methyl ester. are mixed and added in distilled water 50ml, ultrasonic disruption instrument 750w effect 30min, ultrasonic dispersion 5 minutes (300w), 30 DEG C of lasting stirring reactions 9 hours, vacuum drying at 50 DEG C, obtains polymethyl acrylate-chitosan nano complex powder body.
Polymethyl acrylate-chitosan nano complex powder body 30g of preparation is added in guar gum hydrogel 50g, add methyl salicylate 17.5g, heparin sodium 40IU, allantoin 1g, menthol 0.5g, Camphora 0.5g, Borneolum Syntheticum 0.5g simultaneously, continue to stir 3 hours, this mixed gel is put into 40 DEG C of incubators 3 days, and the gel of getting after solidifying is scar note glue (being composite emulsion scar note).
Embodiment bis-
Under 25 DEG C of conditions, get 1mmol/L boric acid 1L, add 1mmol/L NaOH, while utilizing PH meter to regulate PH=11, stop adding in the process adding, react after 10 minutes, add guar gum 50g, 25 DEG C are reacted and obtain guar gum hydrogel half an hour.
30g chitosan, 15g acrylic acid methyl ester. are mixed and added in distilled water 50ml, ultrasonic disruption instrument 750w effect 30min, ultrasonic dispersion 5 minutes (300w), 30 DEG C of lasting stirring reactions 8 hours, vacuum drying at 50 DEG C, obtains polymethyl acrylate-chitosan nano complex powder body.
Polymethyl acrylate-chitosan nano complex powder body 42g of preparation is added in guar gum hydrogel 50g, add methyl salicylate 6g, heparin sodium 50IU, allantoin 1g, Borneolum Syntheticum 1g simultaneously, continue to stir 3 hours, this mixed gel is put into 40 DEG C of incubators 3 days, and the gel of getting after solidifying is scar note glue (being composite emulsion scar note).
Embodiment tri-
Under 25 DEG C of conditions, get 1mmol/L boric acid 1L, add 1mmol/L NaOH, while utilizing PH meter to regulate PH=11, stop adding in the process adding, react after 10 minutes, add guar gum 50g, 25 DEG C are reacted and obtain guar gum hydrogel half an hour.
20g chitosan, 10g acrylic acid methyl ester. are mixed and added in distilled water 50ml, ultrasonic disruption instrument 750w effect 30min, ultrasonic dispersion 5 minutes (300w), 30 DEG C of lasting stirring reactions 9 hours, vacuum drying at 50 DEG C, obtains polymethyl acrylate-chitosan nano complex powder body.
Polymethyl acrylate-chitosan nano complex powder body 30g of preparation is added in guar gum hydrogel 52g, add methyl salicylate 14.5g, allantoin 2g, Borneolum Syntheticum 1.5g simultaneously, continue to stir 4 hours, this mixed gel is put into 40 DEG C of incubators 3 days, and the gel of getting after solidifying is scar note glue (being composite emulsion scar note).
Embodiment tetra-
Under 25 DEG C of conditions, get 1mmol/L boric acid 1L, add 1mmol/L NaOH, while utilizing PH meter to regulate PH=11, stop adding in the process adding, react after 10 minutes, add guar gum 50g, 25 DEG C are reacted and obtain guar gum hydrogel half an hour.
33g chitosan, 11g acrylic acid methyl ester. are mixed and added in distilled water 50ml, ultrasonic disruption instrument 750w effect 30min, ultrasonic dispersion 5 minutes (300w), 30 DEG C of lasting stirring reactions 9 hours, vacuum drying at 50 DEG C, obtains polymethyl acrylate-chitosan nano complex powder body.
Polymethyl acrylate-chitosan nano complex powder body 42g of preparation is added in guar gum hydrogel 40g, add methyl salicylate 15.5g, heparin sodium 40IU, allantoin 1g, menthol 0.5g, Borneolum Syntheticum 1g simultaneously, continue to stir 3 hours, this mixed gel is put into 40 DEG C of incubators 3 days, and the gel of getting after solidifying is scar note glue (being composite emulsion scar note).
Embodiment five
Under 25 DEG C of conditions, get 1mmol/L boric acid 1L, add 1mmol/L NaOH, while utilizing PH meter to regulate PH=11, stop adding in the process adding, react after 10 minutes, add guar gum 50g, 25 DEG C are reacted and obtain guar gum hydrogel half an hour.
33g chitosan, 11g acrylic acid methyl ester. are mixed and added in distilled water 50ml, ultrasonic disruption instrument 750w effect 30min, ultrasonic dispersion 5 minutes (300w), 30 DEG C of lasting stirring reactions 8 hours, vacuum drying at 50 DEG C, obtains polymethyl acrylate-chitosan nano complex powder body.
Polymethyl acrylate-chitosan nano complex powder body 45g of preparation is added in guar gum hydrogel 35g, add methyl salicylate 13.5g, allantoin 2g, menthol 1.5g, Camphora 1.5g, Borneolum Syntheticum 1.5g simultaneously, continue to stir 4 hours, this mixed gel is put into 40 DEG C of incubators 3 days, and the gel of getting after solidifying is scar note glue (being composite emulsion scar note).
Embodiment six
Under 25 DEG C of conditions, get 1mmol/L boric acid 1L, add 1mmol/L NaOH, while utilizing PH meter to regulate PH=11, stop adding in the process adding, react after 10 minutes, add guar gum 50g, 25 DEG C are reacted and obtain guar gum hydrogel half an hour.
33g chitosan, 11g acrylic acid methyl ester. are mixed and added in distilled water 50ml, ultrasonic disruption instrument 750w effect 30min, ultrasonic dispersion 5 minutes (300w), 30 DEG C of lasting stirring reactions 9 hours, vacuum drying at 50 DEG C, obtains polymethyl acrylate-chitosan nano complex powder body.
Polymethyl acrylate-chitosan nano complex powder body 39g of preparation is added in guar gum hydrogel 45g, add methyl salicylate 12g, heparin sodium 40IU, allantoin 1g, menthol 1g, Camphora 1g, Borneolum Syntheticum 1g simultaneously, continue to stir 4 hours, this mixed gel is put into 40 DEG C of incubators 3 days, and the gel of getting after solidifying is scar note glue (being composite emulsion scar note).
Embodiment seven
Under 25 DEG C of conditions, get 1mmol/L boric acid 1L, add 1mmol/L NaOH, while utilizing PH meter to regulate PH=11, stop adding in the process adding, react after 10 minutes, add guar gum 50g, 25 DEG C are reacted and obtain guar gum hydrogel half an hour.
20g chitosan, 10g acrylic acid methyl ester. are mixed and added in distilled water 50ml, ultrasonic disruption instrument 750w effect 30min, ultrasonic dispersion 5 minutes (300w), 30 DEG C of lasting stirring reactions 9 hours, vacuum drying at 50 DEG C, obtains polymethyl acrylate-chitosan nano complex powder body.
Polymethyl acrylate-chitosan nano complex powder body 30g of preparation is added in guar gum hydrogel 50g, add methyl salicylate 18g, heparin sodium 60IU, allantoin 1g, menthol 1g simultaneously, continue to stir 3 hours, this mixed gel is put into 40 DEG C of incubators 3 days, and the gel of getting after solidifying is scar note glue (being composite emulsion scar note).
Embodiment eight
Under 25 DEG C of conditions, get 1mmol/L boric acid 1L, add 1mmol/L NaOH, while utilizing PH meter to regulate PH=11, stop adding in the process adding, react after 10 minutes, add guar gum 50g, 25 DEG C are reacted and obtain guar gum hydrogel half an hour.
20g chitosan, 10g acrylic acid methyl ester. are mixed and added in distilled water 50ml, ultrasonic disruption instrument 750w effect 30min, ultrasonic dispersion 5 minutes (300w), 30 DEG C of lasting stirring reactions 8 hours, vacuum drying at 50 DEG C, obtains polymethyl acrylate-chitosan nano complex powder body.
Polymethyl acrylate-chitosan nano complex powder body 30g of preparation is added in guar gum hydrogel 50g, add methyl salicylate 17g, heparin sodium 50IU, menthol 1g, Camphora 1g, Borneolum Syntheticum 1g simultaneously, continue to stir 3 hours, this mixed gel is put into 40 DEG C of incubators 3 days, and the gel of getting after solidifying is scar note glue (being composite emulsion scar note).
Embodiment nine
Under 25 DEG C of conditions, get 1mmol/L boric acid 1L, add 1mmol/L NaOH, while utilizing PH meter to regulate PH=11, stop adding in the process adding, react after 10 minutes, add guar gum 50g, 25 DEG C are reacted and obtain guar gum hydrogel half an hour.
33g chitosan, 11g acrylic acid methyl ester. are mixed and added in distilled water 50ml, ultrasonic disruption instrument 750w effect 30min, ultrasonic dispersion 5 minutes (300w), 30 DEG C of lasting stirring reactions 9 hours, vacuum drying at 50 DEG C, obtains polymethyl acrylate-chitosan nano complex powder body.
Polymethyl acrylate-chitosan nano complex powder body 41 of preparation is added in guar gum hydrogel 49g, add methyl salicylate 8g, allantoin 1g, menthol 0.5g, Borneolum Syntheticum 0.5g simultaneously, continue to stir 3 hours, this mixed gel is put into 40 DEG C of incubators 3 days, and the gel of getting after solidifying is scar note glue (being composite emulsion scar note).
Embodiment ten
Under 25 DEG C of conditions, get 1mmol/L boric acid 1L, add 1mmol/L NaOH, while utilizing PH meter to regulate PH=11, stop adding in the process adding, react after 10 minutes, add guar gum 45g, 25 DEG C are reacted and obtain guar gum hydrogel half an hour.
33g chitosan, 11g acrylic acid methyl ester. are mixed and added in distilled water 50ml, ultrasonic disruption instrument 750w effect 30min, ultrasonic dispersion 5 minutes (300w), 30 DEG C of lasting stirring reactions 8 hours, vacuum drying at 50 DEG C, obtains polymethyl acrylate-chitosan nano complex powder body.
Polymethyl acrylate-chitosan nano complex powder body 40g of preparation is added in guar gum hydrogel 45g, add methyl salicylate 7g, heparin sodium 70IU, allantoin 2g, menthol 2g, Camphora 2g, Borneolum Syntheticum 2g simultaneously, continue to stir 3 hours, this mixed gel is put into 40 DEG C of incubators 3 days, and the gel of getting after solidifying is scar note glue (being composite emulsion scar note).
Embodiment 11
Under 25 DEG C of conditions, get 1mmol/L boric acid 1L, add 1mmol/L NaOH, while utilizing PH meter to regulate PH=11, stop adding in the process adding, react after 10 minutes, add guar gum 55g, 25 DEG C are reacted and obtain guar gum hydrogel half an hour.
33g chitosan, 11g acrylic acid methyl ester. are mixed and added in distilled water 50ml, ultrasonic disruption instrument 750w effect 30min, ultrasonic dispersion 5 minutes (300w), 30 DEG C of lasting stirring reactions 9 hours, vacuum drying at 50 DEG C, obtains polymethyl acrylate-chitosan nano complex powder body.
Polymethyl acrylate-chitosan nano complex powder body 40g of preparation is added in guar gum hydrogel 52g, add methyl salicylate 7.5g, heparin sodium 40IU, Borneolum Syntheticum 0.5g simultaneously, continue to stir 3 hours, this mixed gel is put into 40 DEG C of incubators 3 days, and the gel of getting after solidifying is scar note glue (being composite emulsion scar note).
Embodiment 12
Under 25 DEG C of conditions, get 1mmol/L boric acid 1L, add 1mmol/L NaOH, while utilizing PH meter to regulate PH=11, stop adding in the process adding, react after 10 minutes, add guar gum 55g, 25 DEG C are reacted and obtain guar gum hydrogel half an hour.
33g chitosan, 11g acrylic acid methyl ester. are mixed and added in distilled water 50ml, ultrasonic disruption instrument 750w effect 30min, ultrasonic dispersion 5 minutes (300w), 30 DEG C of lasting stirring reactions 9 hours, vacuum drying at 50 DEG C, obtains polymethyl acrylate-chitosan nano complex powder body.
Polymethyl acrylate-chitosan nano complex powder body 42g of preparation is added in guar gum hydrogel 52g, add methyl salicylate 6g simultaneously, continue to stir 3 hours, this mixed gel is put into 40 DEG C of incubators 3 days, the gel of getting after solidifying is scar note glue (being composite emulsion scar note).
Embodiment 13
Under 25 DEG C of conditions, get 1mmol/L boric acid 1L, add 1mmol/L NaOH, while utilizing PH meter to regulate PH=11, stop adding in the process adding, react after 10 minutes, add guar gum 55g, 25 DEG C are reacted and obtain guar gum hydrogel half an hour.
33g chitosan, 11g acrylic acid methyl ester. are mixed and added in distilled water 50ml, ultrasonic disruption instrument 750w effect 30min, ultrasonic dispersion 5 minutes (300w), 30 DEG C of lasting stirring reactions 9 hours, vacuum drying at 50 DEG C, obtains polymethyl acrylate-chitosan nano complex powder body.
Polymethyl acrylate-chitosan nano complex powder body 42g of preparation is added in guar gum hydrogel 52g, add methyl salicylate 6g simultaneously, continue to stir 3 hours, this mixed gel is put into 40 DEG C of incubators 3 days, the gel of getting after solidifying is scar note glue (being composite emulsion scar note).
Embodiment 14
Under 25 DEG C of conditions, get 1mmol/L boric acid 1L, add 1mmol/L NaOH, while utilizing PH meter to regulate PH=11, stop adding in the process adding, react after 10 minutes, add guar gum 55g, 25 DEG C are reacted and obtain guar gum hydrogel half an hour.
20g chitosan, 10g acrylic acid methyl ester. are mixed and added in distilled water 50ml, ultrasonic disruption instrument 750w effect 30min, ultrasonic dispersion 5 minutes (300w), 30 DEG C of lasting stirring reactions 9 hours, vacuum drying at 50 DEG C, obtains polymethyl acrylate-chitosan nano complex powder body.
Polymethyl acrylate-chitosan nano complex powder body 30g of preparation is added in guar gum hydrogel 52g, add methyl salicylate 18g simultaneously, continue to stir 3 hours, this mixed gel is put into 40 DEG C of incubators 3 days, the gel of getting after solidifying is scar note glue (being composite emulsion scar note).
Embodiment 15
Under 25 DEG C of conditions, get 1mmol/L boric acid 1L, add 1mmol/L NaOH, while utilizing PH meter to regulate PH=11, stop adding in the process adding, react after 10 minutes, add guar gum 55g, 25 DEG C are reacted and obtain guar gum hydrogel half an hour.
33g chitosan, 11g acrylic acid methyl ester. are mixed and added in distilled water 50ml, ultrasonic disruption instrument 750w effect 30min, ultrasonic dispersion 5 minutes (300w), 30 DEG C of lasting stirring reactions 9 hours, vacuum drying at 50 DEG C, obtains polymethyl acrylate-chitosan nano complex powder body.
Polymethyl acrylate-chitosan nano complex powder body 42g of preparation is added in guar gum hydrogel 40g, add methyl salicylate 18g simultaneously, continue to stir 3 hours, this mixed gel is put into 40 DEG C of incubators 3 days, the gel of getting after solidifying is scar note glue (being composite emulsion scar note).
Embodiment 16
Under 25 DEG C of conditions, get 1mmol/L boric acid 1L, add 1mmol/L NaOH, while utilizing PH meter to regulate PH=11, stop adding in the process adding, react after 10 minutes, add guar gum 55g, 25 DEG C are reacted and obtain guar gum hydrogel half an hour.
33g chitosan, 11g acrylic acid methyl ester. are mixed and added in distilled water 50ml, ultrasonic disruption instrument 750w effect 30min, ultrasonic dispersion 5 minutes (300w), 30 DEG C of lasting stirring reactions 9 hours, vacuum drying at 50 DEG C, obtains polymethyl acrylate-chitosan nano complex powder body.
Polymethyl acrylate-chitosan nano complex powder body 39g of preparation is added in guar gum hydrogel 49g, add methyl salicylate 12g simultaneously, continue to stir 3 hours, this mixed gel is put into 40 DEG C of incubators 3 days, the gel of getting after solidifying is scar note glue (being composite emulsion scar note).
Embodiment 17
Under 25 DEG C of conditions, get 1mmol/L boric acid 1L, add 1mmol/L NaOH, while utilizing PH meter to regulate PH=11, stop adding in the process adding, react after 10 minutes, add guar gum 55g, 25 DEG C are reacted and obtain guar gum hydrogel half an hour.
33g chitosan, 11g acrylic acid methyl ester. are mixed and added in distilled water 50ml, ultrasonic disruption instrument 750w effect 30min, ultrasonic dispersion 5 minutes (300w), 30 DEG C of lasting stirring reactions 9 hours, vacuum drying at 50 DEG C, obtains polymethyl acrylate-chitosan nano complex powder body.
Polymethyl acrylate-chitosan nano complex powder body 38g of preparation is added in guar gum hydrogel 45g, add methyl salicylate 17g simultaneously, continue to stir 3 hours, this mixed gel is put into 40 DEG C of incubators 3 days, the gel of getting after solidifying is scar note glue (being composite emulsion scar note).
Below zoopery and the human trial checking situation to antipruritic effect of the present invention:
Experiment 1:
The preparation of 1.1 Cavia porcellus pruritus models: before experiment, 48h is first by the cropping of right back Cavia porcellus instep, and depilatory loses hair or feathers, the about 1cm of area
2, experiment abraded depilation place skin with fine sandpaper the same day more gently, made it rubescent, but taking not hemorrhage as degree, smeared 0.05% histamine phosphate 30 μ L/ for each group in site of injury, and in 30min, there is licking the action of right back sufficient wound site in Cavia porcellus;
The 1.2 scar note glue that use embodiment mono-to prepare are made 3 × 1 × 0.3cm lamellar, wrap up right foot, and as experimental group, matched group is left intact.Record each group Cavia porcellus (5 every group) in 10min and lick the number of times (lick continuously and lick to occurring minibreak, lick licking 1 calculating) of licking right foot reaction; Result is as follows:
The Cavia porcellus of histamine induction is licked to the impact of foot reaction
As calculated: suppression ratio=49.5%(suppression ratio=(matched group-scar note glue group)/matched group × 100%)
Experiment 2:
2.1ICR mice (the title (being equivalent to BALB/c mouse) of a kind of mice, purchased from Third Military Medical University's Experimental Animal Center), male 10, the about 20g of body weight, is divided into matched group and scar note glue group at random, every group 5, the conventional preserved skin depilation in back, is prepared into 3 × 3 × 0.3cm lamellar by scar note glue (being prepared by embodiment bis-), the skin after the depilation of parcel mouse back, two groups are all given 4-aminopyridine lmgkg in depilation place subcutaneous injection, record and in mice 10min, lick body number of times.
The ICR Mice licking response number of times of 4-aminopyridine induction
As calculated: suppression ratio=58.60%(suppression ratio=(matched group-scar note glue group)/matched group × 100%)
2.2 statistic analysis result are as Fig. 3, and statistical analysis shows: p < 0.0001, prompting scar note glue group Mice licking response number of times is obviously few compared with matched group, the clear and definite antipruritic effect of scar note glue.
Experiment 3:
3.1 choose hypertrophic cicatrix pruritus patient 20 examples, inclusive criteria: 1) burn wound's area 10% left and right, and there is scar hyperplasia in half a year in wound healing, and pruritus is obvious; 2) 18~65 one full year of life of age, male or female; 3) get rid of following case: other serious diseases patients such as severe cardiac, brain, liver, kidney; Spirit, patient with nervous system disease; Tumor patient; Gestation, women breast-feeding their children; Compliance is poor, the person that is difficult to completed treatment; Serious immunodeficiency person; Drug abuse or drugs person.
Patient is divided at random experimental group and matched group by 3.2, every group of 10 people, measure every patient's cicatrix region area, scar note glue (being prepared by embodiment tri-) is prepared into area to experimental group and cicatrix area is similar, thickness is about 0.3cm size (be every square decimeter and use the about 10g of scar note glue).The rear hypertrophic cicatrix of dry combustion method wound and wound surface healing region is cleaned and wiped to warm water gently, and glue is naturally bonding, and used continuously 24 hours every day, changes every other day scar note glue one time.Matched group will not any processing.
3.3 evaluate by the following universal standard Relationship between maternal pruritus after using for 1,3,5,7 day, and calculate every patient's of four days average mark:
Pruritus standards of grading: 0 point: without pruritus.1 point: accidental pruritus.2 points: discontinuity pruritus, has symptom fluctuation.3 points: persistence pruritus, day and night unchanged.
3.4 results are as follows:
Patient's pruritus scoring (meansigma methods)
Referring to Fig. 4, statistic analysis result is shown: burn wound and wound surface healing after scar note glue is processed after, hypertrophic cicatrix accompanies violent pruritus patient's pruritus scoring significantly to decline, and patient's pruritus symptom alleviates.