CN103335983A - Quasi-automatic in-vitro detecting equipment used for detecting specific protein by turbidimetry and method thereof - Google Patents
Quasi-automatic in-vitro detecting equipment used for detecting specific protein by turbidimetry and method thereof Download PDFInfo
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- CN103335983A CN103335983A CN2013102820547A CN201310282054A CN103335983A CN 103335983 A CN103335983 A CN 103335983A CN 2013102820547 A CN2013102820547 A CN 2013102820547A CN 201310282054 A CN201310282054 A CN 201310282054A CN 103335983 A CN103335983 A CN 103335983A
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Abstract
The invention discloses quasi-automatic in-vitro detecting equipment used for detecting a specific protein by turbidimetry and a method thereof. The equipment comprises a detecting bench; the detecting bench is provided with a photoelectric detection module with temperature control and stirring functions, a mechanical arm, a first loading pump, a second loading pump, a first container used for accommodating a first detecting reagent, and a second container used for accommodating a second detecting reagent; a reaction vessel used for pre-loading the first detecting reagent is arranged on the photoelectric detection module; the mechanical arm is provided with a first sampling needle used for loading the first detecting reagent and a second sampling needle used for loading the second detecting reagent. Automatic loading of the first detecting reagent and the second detecting reagent is realized by using the equipment, so that influence to the detecting results caused by volatilization of the first detecting reagent is avoided, labor cost of the specific protein detection is saved, artificial monitoring is unnecessary, and influence to the detecting results caused by the mistakes made in manual adding process of the detecting reagents are avoided as far as possible.
Description
Technical field
The present invention relates to in-vitro diagnosis equipment, in particular the outer checkout equipment of a kind of accurate automaton that adopts turbidimetry to measure specific protein and method.
Background technology
So-called specific protein refers to have certain physiological function in the body, plays those specific proteins of important pathology sense when morbid state again.By these specific proteins being detected the physiological situation that to determine patient's body usually.Existing specific protein can be used for following clinical detection: immune dysfunction (detecting IgA, IgG, IgM, complement C3, complement C4, κ light chain, lambda light chain); Inflammatory conditions monitoring (detecting C-reactive protein, α acidoglycoprotein, haptoglobin, CER); The monitoring of rheumatism (detection type rheumatism factor R F, antistreptolysin O (ASO), C-reactive protein) cardiovascular function (detects super quick C-reactive protein, aPoA 1, apolipoprotein B, lipoprotein a); Renal function detects (detecting microdose urine protein, transferrins, α 1-microglobulin, β2Wei Qiudanbai, IgG); Nutrition condition monitoring (detecting albumin, prealbumin, transferrins); Blood disease (detecting antithrombin, transferrins, haptoglobin); The nervous system disease (detecting albumin, alpha2 Macroglobulin, IgA, IgG, IgM) etc.
At present, the common methods of measuring specific protein is turbidimetry, for example scattering turbidimetry method or turbidimetry, this method is that detected sample is added to earlier among the reagent R1, reaction a period of time adds reagent R2 again, after reacting a period of time again, the turbidity of measuring reaction product changes, and determines the content of specific protein in the detected sample.
Existing equipment based on turbidimetry detection specific protein has two kinds, a kind of is large automatic Biochemical Analyzer, usually be used in clinical laboratory or the centralab of big-and-middle-sized hospital, another kind of small-sized semi-automatic checkout equipment generally is used in clinical department or She Kang center.
Small-sized semi-automatic specific protein checkout equipment as shown in Figure 1 at present, monitor station 10 is provided with the Photoelectric Detection module 20 of a plurality of band temperature controls and agitating function, the testing staff is equipped with detected sample S in the reaction cup 21 that detects reagent R1 manual the adding in advance during measurement, again reaction cup 21 is inserted in the Photoelectric Detection module 20, two kinds of materials are reacted a period of time in reaction cup, and then will detect in the reagent R2 adding reaction cup 21, react a period of time again, measure the turbidity of reaction product in the reaction cup by the microcontroller of checkout equipment, thereby calculate the content of specific protein in the detected sample.Yet this detection mode exists following defective:
1) detecting reagent R2 needs the testing staff manually to add, because reaction cup is the upper end tapered opening, the lower end is chamber, Vertical Narrow footpath, manually splash into when detecting reagent, very easily because testing staff's misoperation, part is detected on the inwall that reagent drops in reaction cup, make the quantity not sufficient of the detection reagent of participating in reaction, and then influence testing result;
2) addition of detection reagent R2 generally is several microlitres, and operating personnel are had very high technical ability requirement, and careless slightly will causing detected the reagent dosage deficiency, very easily produces error, thereby influences the accuracy of measurement result.
3) reaction needed a period of time of detection reagent R1 and detected sample, the instrument alert adds when detecting reagent R2, because detecting the speed of reagent, each operating personnel's skilled operation degree or adding can have nothing in common with each other, therefore, it is different that this can make detection reagent and the reaction time of detected sample exist, and causes between the testing result also can there are differences; Simultaneously, the mode of this artificial interpolation has increased testing staff's workload, and is prone to monitoring error and influences testing result.
4) detect reagent R1 and be contained in the reaction cup in advance usually, can not produce certain volatilization if do not use for a long time, the effective constituent that detects reagent R1 is reduced, to such an extent as to influence final testing result.
Therefore, prior art has yet to be improved and developed.
Summary of the invention
At the above-mentioned defective of prior art, the invention provides the outer checkout equipment of a kind of accurate automaton that adopts turbidimetry to measure specific protein and method, realize that first detects the automatic loading that reagent and second detects reagent, and then realize the robotization that specific protein detects.
The technical scheme that technical solution problem of the present invention adopts is as follows:
The outer checkout equipment of a kind of accurate automaton that adopts turbidimetry to measure specific protein, comprise a monitor station, described monitor station is provided with the Photoelectric Detection module of band temperature control and agitating function, mechanical arm, first load pump, second load pump, hold first second container that detects first container of reagent and hold the second detection reagent, be placed with reaction cup at described Photoelectric Detection module, be separately installed with for loading first second sample needle that detects first sample needle of reagent and be used for loading the second detection reagent at described mechanical arm, the two ends of described first load pump connect described first sample needle and first container respectively, and the two ends of described second load pump connect described second sample needle and second container respectively.
Described employing turbidimetry is measured the outer checkout equipment of accurate automaton of specific protein, and wherein, described Photoelectric Detection module includes several;
Described employing turbidimetry is measured the outer checkout equipment of accurate automaton of specific protein, wherein, described Photoelectric Detection module on monitor station linearly or circular arc arrange.
Described employing turbidimetry is measured the outer checkout equipment of accurate automaton of specific protein, and wherein, described first sample needle is fixedly mounted on the described mechanical arm, and described second sample needle is slidingly mounted on the mechanical arm.
Described employing turbidimetry is measured the outer checkout equipment of accurate automaton of specific protein, and wherein, described first sample needle and second sample needle all are slidingly mounted on the described mechanical arm, and connect by a slide unit.
Described employing turbidimetry is measured the outer checkout equipment of accurate automaton of specific protein, and wherein, described first load pump and second load pump are fixed displacement pump.
Described employing turbidimetry is measured the outer checkout equipment of accurate automaton of specific protein, and wherein, described fixed displacement pump is ram pump or syringe pump.
Described employing turbidimetry is measured the outer checkout equipment of accurate automaton of specific protein, wherein, described Photoelectric Detection module comprises a light source that is arranged on reaction cup one side, is arranged on the reaction cup opposite side and light source is the photoelectric sensor that predetermined angular arranges over against the optical filter that arranges with described light source.
A kind of accurate external detection method automatically that adopts turbidimetry to measure specific protein wherein, is fixedly mounted on the described mechanical arm and second sample needle when being slidingly mounted on the described mechanical arm at described first sample needle, comprises step:
Microprocessor controls starts first load pump, draws first and detect reagent from first container, and join in the reaction cup by first sample needle;
After in described reaction cup, adding detected sample, reaction cup is placed mixing on the Photoelectric Detection module, and monitor the reaction time of the first detection reagent and detected sample;
When monitoring the first detection reagent and the reaction time of detected sample in reaction cup then, microprocessor controls second sample needle moves along mechanical arm, moving to above the reaction cup, start second load pump, the detection of second in second container reagent is joined in the described reaction cup by described second sample needle react;
Determine the content of specific protein in the detected sample by the turbidity of measuring reactive material in the reaction cup.
A kind of accurate external detection method automatically that adopts turbidimetry to measure specific protein wherein, all is slidingly mounted on the described mechanical arm and by a slide unit at described first sample needle and second sample needle and is connected, and comprises step:
In reaction cup, add detected sample and reaction cup is placed on the Photoelectric Detection module;
The microprocessor controls slide unit moves, and first sample needle is aimed at reaction cup, starts first load pump, draws first and detect reagent from first container, and join in the reaction cup by first sample needle;
By described Photoelectric Detection module mixing, and monitored for first reaction time of detecting reagent and detected sample;
When monitoring the first detection reagent and the reaction time of detected sample in reaction cup then, the microprocessor controls slide unit moves along mechanical arm, described second sample needle is aimed at reaction cup, start second load pump, from second container, draw second and detect reagent, and join in the reaction cup by second sample needle and to react;
Determine the content of specific protein in the detected sample by the turbidity of measuring reactive material in the reaction cup.
Employing turbidimetry provided by the present invention is measured the outer checkout equipment of accurate automaton and the method for specific protein, its technical advantages that reaches is: pass through mechanical arm, the setting of two load pump and corresponding two sample needles, realized that respectively first detects the automatic loading that reagent and second detects reagent, avoided the first detection reagent to influence the problem of testing result because volatilization causes the reagent content, not only saved the cost of labor that specific protein detects, the operation error of having avoided artificial dropping to detect reagent impacts testing result, but also improved the detection efficiency of specific protein, have vast market prospect.
Description of drawings
Fig. 1 is the structural representation of specific protein checkout equipment in the prior art.
Fig. 2 is the structural representation that employing turbidimetry provided by the invention is measured the outer checkout equipment of accurate automaton of specific protein.
Fig. 3 is the structural representation of the another embodiment of the employing turbidimetry provided by the invention outer checkout equipment of accurate automaton of measuring specific protein.
Fig. 4 is the structural representation that employing turbidimetry provided by the invention is measured Photoelectric Detection module in the outer checkout equipment of accurate automaton of specific protein.
Fig. 5 is the structural representation that employing turbidimetry provided by the invention is measured reaction cup in the outer checkout equipment of accurate automaton of specific protein.
Fig. 6 is the accurate process flow diagram of a preferred embodiment of external detection method automatically that employing turbidimetry provided by the invention is measured specific protein.
Fig. 7 is the accurate process flow diagram of the another preferred embodiment of external detection method automatically that employing turbidimetry provided by the invention is measured specific protein.
Embodiment
For making purpose of the present invention, technical scheme and advantage clearer, clear and definite, below develop simultaneously with reference to accompanying drawing that the present invention is described in more detail for embodiment.Should be appreciated that specific embodiment described herein only in order to explaining the present invention, and be not used in restriction the present invention.
The invention provides the outer checkout equipment of a kind of accurate automaton that adopts turbidimetry to measure specific protein, as shown in Figure 2, comprise a monitor station 100, described monitor station 100 is provided with the Photoelectric Detection module 110 of band temperature control and agitating function, and being placed with reaction cup 200 at Photoelectric Detection module 110, reaction cup 200 is used for detecting as the first detection reagent, detected sample and second reaction vessel of reagent.On described monitor station 100, also be provided with a mechanical arm 101, the first load pump 121, second load pump 122, hold first second container 105 that detects first container 104 of reagent and hold the second detection reagent.Mechanical arm 101 by screw 140(but be not limited to) be fixed on the monitor station 100, first sample needle 102 and second sample needle 103 also are installed on mechanical arm 101, the two ends of first load pump 121 connect first sample needle 102 and first container 104 respectively, and second load pump 122 directly connects second sample needle 103.Be connected by conduit 120 between first load pump 121 and first sample needle 102 and first container 104 and between second load pump 122 and second sample needle 103, but be not limited to conduit.First detects reagent holds by the first special container, when detecting than prepackage in reaction cup first, can avoid first to detect the volatilization problem of reagent, and then avoid influencing final testing result.
In the present embodiment, the mounting means of first sample needle 102 and second sample needle 103 has two kinds, first kind of mounting means is: first sample needle 102 is fixedly mounted on the mechanical arm 101, and second sample needle 103 is slidingly mounted on the mechanical arm 101, first sample needle 102 is fixed on the appropriate location of mechanical arm, and this position is preferably the two ends of mechanical arm, and second sample needle 103 is installed on the mechanical arm by a slide unit, therefore, second sample needle 103 can move along mechanical arm 101.This kind mounting means can realize that the first detection reagent drips in the fixed position, second detects reagent loads automatically, when loading, need manually reaction cup to be positioned over the first sample needle place and drip the first detection reagent, and move to second container that holds the second detection reagent by second sample needle along mechanical arm, draw second and detect reagent.
Second kind of mounting means is: as shown in Figure 3, first sample needle 102 and second sample needle 103 all are slidingly mounted on the mechanical arm 101, and the two connects by a slide unit 106.Therefore first sample needle 102 and second sample needle 103 all can move along mechanical arm 101.When realizing detecting the automatic loading of reagent by this mounting means, at first first sample needle moves to the reaction cup place along mechanical arm, detecting reagent with first draws from first container and joins in the reaction cup, when loading the second detection reagent, second sample needle moves to the second container place that holds the second detection reagent along mechanical arm, therefrom draw second and detect reagent, and then move to the reaction cup place, detect reagent with second and join in the reaction cup.
Above-mentioned two kinds of mounting meanss have all been realized two kinds of automatic loadings that detect reagent, it is some difference of load mode, in first kind of mounting means, because first sample needle is fixedly mounted on the mechanical arm, therefore need artificial mobile response cup, to finish the loading of the first detection reagent, and in second kind of mounting means, because therefore first sample needle is connected by a slide unit with second sample needle, and is installed on the mechanical arm, together, first sample needle and second sample needle all can move along mechanical arm, therefore, second kind of mounting means need not manually participate in respect to first kind of mounting means, and automaticity is higher.
Preferably, the structure of Photoelectric Detection module of the present invention as shown in Figure 4, this Photoelectric Detection module comprise a light source 111 that is arranged on reaction cup 200 1 sides, be arranged on reaction cup 200 opposite sides be the photoelectric sensor 113 of predetermined angular with light source over against the optical filter 112 that arranges with the light horizontal direction of living in that light source is launched, the angle of photoelectric sensor and level is α, the big I of α is adjusted according to actual needs, preferable range is [0 °, 30 °].Described light source is used for sending the detection light beam, and described optical filter 112 is used for the detection light beam through reaction cup scattering or transmission is filtered, and described photoelectric sensor 113 is used for receiving the detection light beam after optical filter filters.Detection light beam irradiates detection reagent and detected sample fully react the reaction cup after finishing, owing to contain certain particle in suspension in the reactive material in the reaction cup, when the detection light beam contains the medium of particle in suspension by one, because particle in suspension is to scattering of light effect and optionally absorption, the transmission light intensity is weakened or the scattered intensity enhancing, by the detection to transmitted light or scattered intensity, determine the turbidity of reactive material, and then the content of material composition in the mensuration detected sample, realize the detection of specific protein content.According to the difference of deciding albumen, realize the detection of different test indexs.
Further, checkout equipment provided by the invention can detect for single channel, can be multi-channel detection, single channel only arranges a Photoelectric Detection module on monitor station when detecting, and during multi-channel detection, present embodiment includes several Photoelectric Detection modules, and this moment, the Photoelectric Detection module was arranged in a linear at monitor station, the quantity of Photoelectric Detection module is arranged as required, usually but be not limited to 4 to 8, the modes of emplacement of this straight-line has clearly advantage with respect to existing circular modes of emplacement.Reaction cup structural representation as shown in Figure 5, reaction cup 200 is generally the upper end pyramidal structure, opening is square, the lower end is vertical cavity, vertically cavity diameter is less, these reaction cup are placed on the Photoelectric Detection module, if adopt circular the placement, the placement orientation difference of each reaction cup then, splashing in the process that detects reagent or detected sample to one of them, be easy to cause detection reagent or detected sample to be dropped on the inwall of reaction cup, influencing testing result, and circular structure of placing is complicated, and cost is higher.Can reduce as much as possible and detect reagent or detected sample and dropped in probability on the reaction cup inwall, reduce the error risk and adopt straight-line that the Photoelectric Detection module is set.Simultaneously, straight-line arranges the control that the Photoelectric Detection module also can be simplified sample needle greatly.Certainly, the Photoelectric Detection module is not limited to adopt line spread at monitor station, also can adopt circular arc to arrange or other arrangement modes.
The arrangement mode of the structure of mechanical arm of the present invention and Photoelectric Detection module is adaptive, in the present embodiment, arrange because the Photoelectric Detection module is orthoscopic, therefore mechanical arm 101 of the present invention is preferably linear structure, and this linear structure can be simplified the movement of sample needle on mechanical arm.Mechanical arm can be arranged on but be not limited to the Photoelectric Detection module directly over, directly over being preferably, like this, sample needle just can be aimed at reaction cup, is convenient to detect the interpolation of reagent.
In order to realize that well first detects the automatic loading of reagent and the second detection reagent and the robotization control of correlated process, such as the stirring control of Photoelectric Detection module, timing controlled etc., meeting of the present invention arranges a microcontroller 130 with timing function at this vitro detection equipment, this microcontroller 130 is used for controlling this vitro detection equipment automatically, comprises that specifically first sample needle or described second sample needle move and control described first load pump absorption first at mechanical arm and detect reagent and described second load pump absorption, the second detection reagent of control; When controlling first load pump absorption, the first detection reagent, because first load pump connects first sample needle and first container that holds the first detection reagent respectively by conduit, and the difference of mounting means.Therefore, at first kind of mounting means, reaction cup is placed on the first sample needle place, by automatic control knob, start the automatic loading of the first detection reagent, this moment, microcontroller can start first load pump, directly drew the first an amount of detection reagent from first container, and was added drop-wise in the reaction cup by first sample needle.Manually add detected sample afterwards, and be placed on mixing on the Photoelectric Detection module, microcontroller carries out timing to first reaction time of detecting reagent and detected sample, and can the reaction time then, control second sample needle and move to the second container place that holds the second detection reagent along mechanical arm, start second load pump, from second container, draw the second an amount of detection reagent by second sample needle that connects, and by second sample needle second detection reagent is splashed in the reaction cup.At second kind of mounting means, need not manually to pick and place reaction cup, reaction cup is placed on the Photoelectric Detection module, microprocessor controls first sample needle moves to the reaction cup place, by automatic control knob, start the automatic loading of the first detection reagent, this moment, microcontroller can start first load pump, directly from first container, draw the first an amount of detection reagent, and be added drop-wise in the reaction cup by first sample needle.Manually add detected sample afterwards, and be placed on mixing on the Photoelectric Detection module, microcontroller carries out timing to first reaction time of detecting reagent and detected sample, and can the reaction time then, control second sample needle and move to the second container place that holds the second detection reagent along mechanical arm, start second load pump, from second container, draw the second an amount of detection reagent by second sample needle that connects, and by second sample needle second detection reagent is splashed in the reaction cup.So just realized well that first detects the automatic control that reagent and second detects reagents loaded.Promote the intellectuality that detects.
In this embodiment, connect first load pump owing to hold first first container that detects reagent by conduit, and second container that holds the second detection reagent connects second load pump by conduit, therefore the concrete placement location of first container and second container can't constitute influence to the detection implementation process of this vitro detection equipment, therefore do not do restriction for the concrete placement location of first container and second container at this, preferably be placed on and be easy to pick and place and do not influence the position that sample needle moves.Simultaneously, deposit because the second detection reagent need freeze, therefore, second container of present embodiment is preferably and is positioned in the refrigerating plant.
Preferably, draw the first detection reagent and the second detection reagent respectively in order better to implement first load pump and second load pump, present embodiment can pass through microprocessor controls first load pump and second load pump, control first respectively and detect the uptake that reagent and second detects reagent, so that the testing staff is according to detecting the needs adjustment.
First load pump of the present invention and second load pump are fixed displacement pump, and for example ram pump or syringe pump preferably adopt the high precision injection measurement pump of valveless ram pump or T-valve.Valveless ram pump inside is stupalith, when drawing detection reagent by it detection reagent is sticked in the load pump, influences the subsequent detection result in order to avoid detect for a long time; And the high precision injection measurement pump configuration of T-valve is more complicated, it can at first will detect reagent by a valve and draw in pump, and then discharge detection reagent by another valve, this kind load pump can accurately be controlled the addition that detects reagent, if adopt the high precision injection measurement pump of this T-valve, checkout equipment of the present invention can realize detecting adding more fast and accurately of reagent.And second load pump is not done restriction, can adopt the high precision injection measurement pump of valveless ram pump or T-valve certainly.
Based on above-mentioned vitro detection equipment, the present invention also provides a kind of detection method by above-mentioned vitro detection equipment, at different mounting meanss, comprises different implementation processes.As shown in Figure 6, adopt first sample needle to be fixedly mounted on the mechanical arm and second sample needle when being slidingly mounted on the mechanical arm, comprise step:
Step S101, microprocessor controls start first load pump, draw first and detect reagent from first container, and join in the reaction cup by first sample needle;
Step S102, in described reaction cup, add detected sample after, reaction cup is placed mixing on the Photoelectric Detection module, and monitored for first reaction time of detecting reagent and detected sample;
Step S103, detect reagent and the reaction time of detected sample in reaction cup then when monitoring first, microprocessor controls second sample needle moves along mechanical arm, moving to above the reaction cup, start second load pump, the detection of second in second container reagent is joined in the described reaction cup by described second sample needle react;
Step S104, determine the content of specific protein in the detected sample by the turbidity of measuring reactive material in the reaction cup.
And adopting described first sample needle all to be slidingly mounted on the described mechanical arm with second sample needle and be connected by a slide unit, as shown in Figure 7, comprise step:
Step S201, in reaction cup, add detected sample and reaction cup is placed on the Photoelectric Detection module;
Step S202, microprocessor controls slide unit move, and first sample needle is aimed at reaction cup, start first load pump, draw first and detect reagent from first container, and join in the reaction cup by first sample needle;
Step S203, by described Photoelectric Detection module mixing, and monitored for first reaction time of detecting reagent and detected sample;
Step S204, detect reagent and the reaction time of detected sample in reaction cup then when monitoring first, the microprocessor controls slide unit moves along mechanical arm, described second sample needle is aimed at reaction cup, start second load pump, from second container, draw second and detect reagent, and join in the reaction cup by second sample needle and to react;
Step S205, determine the content of specific protein in the detected sample by the turbidity of measuring reactive material in the reaction cup.
In order to make the above embodiment of the present invention more clear, below in conjunction with specific protein detect a kind of implementation process that the present invention is measured specific protein content by turbidimetry and be specifically described.
This embodiment carries out CRP by the c reactive protein detection kit and detects.CRP is proportionate as acute phase protein rising amplitude and the infected degree of human body.CRP can be used for the antidiastole of bacterium and virus infections.In surperficial healthy population, the concentration of CRP is dangerous directly related with generation coronary heart disease.To resist people's c reactive protein antibody sandwich on latex particle, can with serum in c reactive protein generation agglutinating reaction, form antigen antibody complex, its turbidity height is directly proportional with CRP content in the serum when a certain amount of antibody exists.By detecting transmitted light or scattered intensity, can calculate the content of CRP in the serum with reference to multiple spot calibration calibration curve.When detecting, first of prepackage 600uL detects reagent R1 in the reaction cup, and the detected sample (serum or whole blood) by sample needle absorption 3uL joins in the reaction cup, the Photoelectric Detection module is with its mixing, and place 37 ℃ of incubation a period of times, sample needle moves the second detection reagent R2 that draws 60uL then, and splashes in the reaction cup, the Photoelectric Detection module is with its mixing, and after hatching 10 seconds, read scattered light intensity A1 in continuous 90 seconds, and then calculate corresponding CRP content.The process of above-mentioned detection CRP content only is used for explaining that the present invention measures the method for specific protein content by turbidimetry, and is not used in this method of restriction, because detection method of the present invention can be applied to the detection of multiple specific protein.Such as α acidoglycoprotein, haptoglobin, aPoA 1 etc.
In sum, the outer checkout equipment of a kind of accurate automaton that adopts turbidimetry to measure specific protein provided by the invention and method.The present invention has realized that simultaneously first detects the automatic loading that reagent and second detects reagent, avoided the first detection reagent to influence the problem of testing result because volatilization causes the reagent content, not only saved the cost of labor that specific protein detects, saved the manual monitoring link, but also the operation error of having avoided artificial dropping to detect reagent as much as possible impacts to testing result; The present invention has simultaneously improved the detection efficiency of specific protein, has shortened the detection time of specific protein greatly, has vast market prospect.
Should be understood that application of the present invention is not limited to above-mentioned giving an example, for those of ordinary skills, can be improved according to the above description or conversion that all these improvement and conversion all should belong to the protection domain of claims of the present invention.
Claims (10)
1. outer checkout equipment of the accurate automaton that adopts turbidimetry to measure specific protein, it is characterized in that, comprise a monitor station, described monitor station is provided with the Photoelectric Detection module of band temperature control and agitating function, mechanical arm, first load pump, second load pump, hold first second container that detects first container of reagent and hold the second detection reagent, be placed with reaction cup at described Photoelectric Detection module, be separately installed with for loading first second sample needle that detects first sample needle of reagent and be used for loading the second detection reagent at described mechanical arm, the two ends of described first load pump connect described first sample needle and first container respectively, and the two ends of described second load pump connect described second sample needle and second container respectively.
2. employing turbidimetry according to claim 1 is measured the outer checkout equipment of accurate automaton of specific protein, it is characterized in that described Photoelectric Detection module includes several.
3. employing turbidimetry according to claim 2 is measured the outer checkout equipment of accurate automaton of specific protein, it is characterized in that, described Photoelectric Detection module on monitor station linearly or circular arc arrange.
4. employing turbidimetry according to claim 1 is measured the outer checkout equipment of accurate automaton of specific protein, it is characterized in that described first sample needle is fixedly mounted on the described mechanical arm, and described second sample needle is slidingly mounted on the mechanical arm.
5. employing turbidimetry according to claim 1 is measured the outer checkout equipment of accurate automaton of specific protein, it is characterized in that described first sample needle and second sample needle all are slidingly mounted on the described mechanical arm, and connects by a slide unit.
6. employing turbidimetry according to claim 1 is measured the outer checkout equipment of accurate automaton of specific protein, it is characterized in that described first load pump and second load pump are fixed displacement pump.
7. employing turbidimetry according to claim 6 is measured the outer checkout equipment of accurate automaton of specific protein, it is characterized in that described fixed displacement pump is ram pump or syringe pump.
8. employing turbidimetry according to claim 1 is measured the outer checkout equipment of accurate automaton of specific protein, it is characterized in that described Photoelectric Detection module comprises a light source that is arranged on reaction cup one side, be arranged on the reaction cup opposite side and described light source is the photoelectric sensor that predetermined angular arranges.
9. an accurate external detection method automatically that adopts turbidimetry to measure specific protein is characterized in that, is fixedly mounted on the described mechanical arm and second sample needle when being slidingly mounted on the described mechanical arm at described first sample needle, comprises step:
Microprocessor controls starts first load pump, draws first and detect reagent from first container, and join in the reaction cup by first sample needle;
After in described reaction cup, adding detected sample, reaction cup is placed mixing on the Photoelectric Detection module, and monitor the reaction time of the first detection reagent and detected sample;
When monitoring the first detection reagent and the reaction time of detected sample in reaction cup then, microprocessor controls second sample needle moves along mechanical arm, moving to above the reaction cup, start second load pump, the detection of second in second container reagent is joined in the described reaction cup by described second sample needle react;
Determine the content of specific protein in the detected sample by the turbidity of measuring reactive material in the reaction cup.
10. an accurate external detection method automatically that adopts turbidimetry to measure specific protein is characterized in that, all is slidingly mounted on the described mechanical arm and by a slide unit at described first sample needle and second sample needle to be connected, and comprises step:
In reaction cup, add detected sample and reaction cup is placed on the Photoelectric Detection module;
The microprocessor controls slide unit moves, and first sample needle is aimed at reaction cup, starts first load pump, draws first and detect reagent from first container, and join in the reaction cup by first sample needle;
By described Photoelectric Detection module mixing, and monitored for first reaction time of detecting reagent and detected sample;
When monitoring the first detection reagent and the reaction time of detected sample in reaction cup then, the microprocessor controls slide unit moves along mechanical arm, described second sample needle is aimed at reaction cup, start second load pump, from second container, draw second and detect reagent, and join in the reaction cup by second sample needle and to react;
Determine the content of specific protein in the detected sample by the turbidity of measuring reactive material in the reaction cup.
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| CN106996978A (en) * | 2017-03-01 | 2017-08-01 | 北京九强生物技术股份有限公司 | Alpha2 macroglobulin detection kit and preparation method thereof |
| CN109030612A (en) * | 2018-08-01 | 2018-12-18 | 深圳市华星光电技术有限公司 | Sampling device |
| CN109929735A (en) * | 2019-04-25 | 2019-06-25 | 湖南工业大学 | A kind of plunger type detection of nucleic acids integration cartridge and its detection method |
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| CN109030612A (en) * | 2018-08-01 | 2018-12-18 | 深圳市华星光电技术有限公司 | Sampling device |
| CN109929735A (en) * | 2019-04-25 | 2019-06-25 | 湖南工业大学 | A kind of plunger type detection of nucleic acids integration cartridge and its detection method |
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