CN103333348A - Preparation method and production equipment of carboxylated poly(glycosaminoglycan) medical biogum - Google Patents
Preparation method and production equipment of carboxylated poly(glycosaminoglycan) medical biogum Download PDFInfo
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- CN103333348A CN103333348A CN2013102445541A CN201310244554A CN103333348A CN 103333348 A CN103333348 A CN 103333348A CN 2013102445541 A CN2013102445541 A CN 2013102445541A CN 201310244554 A CN201310244554 A CN 201310244554A CN 103333348 A CN103333348 A CN 103333348A
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Abstract
The invention relates to the production field of medical polymer biological materials and in particular relates to production equipment and a preparation method of carboxylated poly(glycosaminoglycan) medical biogum. The preparation method of the carboxylated poly(glycosaminoglycan) medical biogum comprises the following steps of: under the condition that a stirring frequency is 40-80 r/min, dissolving carboxylated poly(glycosaminoglycan) with water for injection at the temperature of 85-100 DEG C, so as to form a solution; preparing a diluted solution, namely adding the water for injection at the temperature of 85-100 DEG C into a concentrated solution; stirring for 30-55 minutes again while the stirring frequency is constant, and discontinuously discharging the solution at the bottom and adding the discharged solution from the top while stirring, so that macromolecule substances in the carboxylated poly(glycosaminoglycan) are circulated and dissolved; and filtering and then filling the diluted solution. According to the preparation method of carboxylated poly(glycosaminoglycan) medical biogum, the water for injection at the temperature of 85-100 DEG C is used, so that dissolution of carboxylated poly(glycosaminoglycan) can be facilitated; the solution at the bottom is discharged and then added from the top in a diluted solution preparation process, so that the macromolecule substances in carboxylated poly(glycosaminoglycan) are rapidly dissolved and solution preparation time is shortened.
Description
Technical field
The present invention relates to medical polymer biological material produce field, in particular to production unit and the preparation method of the poly-glycosaminoglycan medical bio glue of carboxylation.
Background technology
Among the preparation method of the poly-glycosaminoglycan medical bio glue of a kind of carboxylation in the correlation technique, at first in stainless steel cask, carry out dense joining, by manual stirring the poly-glycosaminoglycan of carboxylation is dissolved in 55 ℃ of waters for injection and forms solution, then the solution after dense joining is injected preparing tank, it is rare process of joining of carrying out solution under 30 rev/mins the condition in stirring frequency, rare join finish after, solution is heated to 65 ℃, last solution can after filtering.In process for preparation, 55 ℃ of low temperature preparations are unfavorable for the dissolving of the poly-glycosaminoglycan of carboxylation, have prolonged the time of configuration solution.
Summary of the invention
The object of the present invention is to provide preparation method and the production unit of the poly-glycosaminoglycan medical bio glue of carboxylation, to solve the above problems.
The preparation method of the poly-glycosaminoglycan medical bio glue of a kind of carboxylation is provided in an embodiment of the present invention, and this method may further comprise the steps:
(1) dense joining: be that the poly-glycosaminoglycan of water for injection dissolving carboxylation with 85-100 ℃ forms solution under 40～80 rev/mins the condition in stirring frequency;
(2) rare joining: at first add for the second time 85-100 ℃ water for injection in the solution after dense joining, feed the steam of water for injection simultaneously in the solution, solution is heated, make the temperature maintenance of solution at 85～100 ℃; Keeping stirring frequency then is under 40～80 rev/mins the condition, to stir again 30-55 minute, and the solution that constantly will be positioned at the bottom when stirring is derived and added by the top, makes macromolecular substance circulation dissolving in the poly-glycosaminoglycan of carboxylation;
(3) with the can after filtering of the solution after rare joining.
The production unit of the poly-glycosaminoglycan medical bio glue of a kind of carboxylation also is provided in an embodiment of the present invention, comprise preparing tank, Y-tube, circulation tube and filtration unit, the bottom of described preparing tank is provided with first output tube, described first output tube, described circulation tube and described filtration unit connect three mouths of pipe of described Y-tube respectively, and described circulation tube is connected with the top of described preparing tank away from an end of described Y-tube; Described circulation tube is provided with pneumatic diaphragm pump; One side of described preparing tank is provided with vapour pipe, and described vapour pipe is connected with steam unit away from an end of described preparing tank;
Described preparing tank is used for: carboxylation is gathered the water for injection that glycosaminoglycan is dissolved in 85-100 ℃, finish dense joining and rare process of joining;
Described circulation tube is used for: provide power by pneumatic diaphragm pump, the solution that constantly will be positioned at the preparing tank bottom is derived again by top adding preparing tank;
Described vapour pipe is used for: the steam to solution feeding water for injection, solution is heated, and keeping solution temperature is 85～100 ℃;
Described filtration unit is used for: solution is can after filtering.
Preparation method and the production unit of the poly-glycosaminoglycan medical bio glue of the carboxylation of the above embodiment of the present invention in present method, are that the dissolving carboxylation is gathered glycosaminoglycan under 40～80 rev/mins the condition in stirring frequency with 85-100 ℃ of water for injection at first.Adopt 85-100 ℃ of water for injection to be conducive to the dissolving that carboxylation is gathered glycosaminoglycan, shortened the time of obtain solution; Then at rare water for injection that continues 85-100 ℃ of adding in the process of joining, feed simultaneously the steam of water for injection in the solution, solution is heated, make the temperature maintenance of solution at 85～100 ℃, and be under 40～80 rev/mins the condition at rotating speed, the solution that constantly will be positioned at the bottom is derived and is added by the top, and the mode of this circulation dissolving cooperates and increases stirring frequency and can make that macromolecular substance dissolves fast in the poly-glycosaminoglycan of carboxylation, has shortened the time of obtain solution.
Description of drawings
Fig. 1 shows the structural representation of the production unit of the poly-glycosaminoglycan medical bio glue of the embodiment of the invention 2 described carboxylations.
Among the figure:
1, preparing tank; 2, circulation tube; 3, pneumatic diaphragm pump; 4, strainer; 5, agitating vane; 6, sanitary pump; 7, Y-tube; 8, first output tube; 9, second output tube.
Embodiment
Also by reference to the accompanying drawings the present invention is described in further detail below by specific embodiment.
Embodiment 1, the preparation method of the poly-glycosaminoglycan medical bio glue of a kind of carboxylation, and this method may further comprise the steps:
(1) dense joining: be that the poly-glycosaminoglycan of water for injection dissolving carboxylation with 85-100 ℃ forms solution under 40～80 rev/mins the condition in stirring frequency.
(2) rare joining: at first add for the second time 85-100 ℃ water for injection in the solution after dense joining, feed the steam of water for injection simultaneously in the solution, solution is heated, make the temperature maintenance of solution at 85～100 ℃; Keeping stirring frequency then is under 40～80 rev/mins the condition, to stir again 30-55 minute, and the solution that constantly will be positioned at the bottom when stirring is derived and added by the top, makes macromolecular substance circulation dissolving in the poly-glycosaminoglycan of carboxylation.
(3) with the can after filtering of the solution after rare joining.
In present method, be the poly-glycosaminoglycan of dissolving carboxylation under 40～80 rev/mins the condition in stirring frequency with 85-100 ℃ of water for injection at first.Adopt 85-100 ℃ of water for injection to be conducive to the dissolving that carboxylation is gathered glycosaminoglycan, shortened the time of obtain solution; Then at rare water for injection that continues 85-100 ℃ of adding in the process of joining, feed simultaneously the steam of water for injection in the solution, solution is heated, make the temperature maintenance of solution at 85～100 ℃, and be under 40～80 rev/mins the condition at rotating speed, the solution that constantly will be positioned at the bottom is derived and is added by the top, and the mode of this circulation dissolving cooperates and increases stirring frequency and can make that macromolecular substance dissolves fast in the poly-glycosaminoglycan of carboxylation, has shortened the time of obtain solution.
Further, in the step (3), the solution after described rare joining need be cooled to 70 ℃～80 ℃ after-filtration.Solution refilters after cooling, has prevented that solution temperature is too high each parts in the filtration unit are caused damage; Solution is cooled in 70 ℃～80 ℃ the temperature range in addition, can the solubleness of raw material not impacted, thereby guarantee that molecule filters, and has strengthened the flowability of liquid under the situation of fully dissolving.
Among this embodiment, describedly densely join process and described rare process of joining is finished in same container.In same container, finish dense joining and rare process of joining, reduced the dense process of joining of original preparation technology stirring and dissolving raw material in stainless steel cask, after reducing this process, reduced the risk of introducing microorganism, prevented that product from existing the situation of microbial contamination to take place in layoutprocedure.
Among this embodiment, described preparation method should be in 1 cubic metres of air the dust number more than 5 microns be no more than under 100 the environment and carry out.Because have the risk of microbial contamination in this product process for preparation, preparing product can guarantee that product is not subjected to microbial contamination under this environment, thereby has guaranteed the quality of product.
Embodiment 2, as shown in Figure 1, the production unit of the poly-glycosaminoglycan medical bio glue of a kind of carboxylation, comprise preparing tank 1, Y-tube 7, circulation tube 2 and filtration unit, the bottom of described preparing tank 1 is provided with first output tube 8, described first output tube 8, described circulation tube 2 and described filtration unit connect three mouths of pipe of described Y-tube 7 respectively, and described circulation tube 2 is connected with the top of described preparing tank 1 away from an end of described Y-tube 7; Described circulation tube 2 is provided with pneumatic diaphragm pump 3; One side of described preparing tank 1 is provided with vapour pipe, and described vapour pipe is connected with steam unit away from an end of described preparing tank 1;
Described preparing tank 1 is used for: carboxylation is gathered the water for injection that glycosaminoglycan is dissolved in 85-100 ℃, finish dense joining and rare process of joining;
Described circulation tube 2 is used for: provide power by pneumatic diaphragm pump 3, the solution that constantly will be positioned at preparing tank 1 bottom is derived again by top adding preparing tank 1;
Described vapour pipe is used for: the steam to solution feeding water for injection, solution is heated, and keeping solution temperature is 85～100 ℃;
Described filtration unit is used for: solution is can after filtering.
This production unit is provided with first output tube 8 in the bottom of preparing tank 1, first output tube 8 is connected with circulation tube 2 by Y-tube 7, circulation tube 2 is realized the self-circulation of solution by pneumatic diaphragm pump 3, the self-circulation process is to provide power by pneumatic diaphragm pump 3, the solution that constantly will be positioned at preparing tank 1 bottom is derived again by top adding preparing tank 1, macromolecular substance in the poly-glycosaminoglycan of carboxylation is dissolved fast, shortened the time of solution preparation; Pneumatic diaphragm pump 3 is applicable to that mainly medium-high viscosity, purity are higher, the liquor of easy pollution, pneumatic diaphragm pump 3 also can be by containing the liquid of particle in addition, because the poly-glycosaminoglycan of the carboxylation of the embodiment of the invention belongs to ultrapure medium, easily pollutes and the liquid of thickness very, so use pneumatic diaphragm pump 3 to guarantee that liquid is not blocked, easy to operate in flow process.
Be provided with stir shaft in the described preparing tank 1, the length of agitating vane is 60cm on the described stir shaft.In the correlation technique, the length of agitating vane 5 is 40cm, increases the length of agitating vane 5, can make macromolecular substance dissolving faster in whipping process in the poly-glycosaminoglycan of carboxylation.Described filtration unit comprises second output tube 9, sanitary pump 6 and millipore filter 4, described sanitary pump 6 is located on described second output tube 9, described millipore filter 4 is connected with the mouth of pipe of described Y-tube 7 by described second output tube 9, and the aperture of described millipore filter 4 is smaller or equal to 0.45 μ m.After solution filters through the millipore filter 4 smaller or equal to 0.45 μ m, but just can has guaranteed the quality of product.
Be equipped with diaphragm valve on described first output tube 8, described circulation tube 2 and described second output tube 9.Diaphragm valve mainly is applicable to ultrapure medium, easily pollutes, very in the operating equipment of liquid, corrodibility or the inert media of thickness, in embodiments of the present invention, the poly-glycosaminoglycan of carboxylation belongs to ultrapure medium, easily pollutes and the liquid of thickness very, so by the circulation of solution in the diaphragm valve control entire equipment, guaranteed that liquid does not have contaminative in course of conveying in the embodiment of the invention.Described preparing tank 1 sidewall is provided with thermal insulation layer.Thermal insulation layer is incubated liquid in the preparing tank 1, and temperature is more high, and the poly-glycosaminoglycan dissolving of carboxylation is more fast, has increased the dissolution rate of the poly-glycosaminoglycan of carboxylation.This production unit also comprises thermometer, and described preparing tank 1 top is provided with the jack that is used with described thermometer, and an end of described thermometer passes described jack and is located in the preparing tank 1.The design of thermometer is that the fluid temperature in the preparing tank 1 is detected, if fluid temperature is too high in the preparing tank 1, liquid causes conduit failure easily in course of conveying.
The above is the preferred embodiments of the present invention only, is not limited to the present invention, and for a person skilled in the art, the present invention can have various changes and variation.Within the spirit and principles in the present invention all, any modification of doing, be equal to replacement, improvement etc., all should be included within protection scope of the present invention.
Claims (10)
1. the preparation method of the poly-glycosaminoglycan medical bio glue of a carboxylation is characterized in that this method may further comprise the steps:
(1) dense joining: be that the poly-glycosaminoglycan of waters for injection dissolving carboxylation with 85～100 ℃ forms solution under 40～80 rev/mins the condition in stirring frequency;
(2) rare joining: at first add for the second time 85-100 ℃ water for injection in the solution after dense joining, feed the steam of water for injection simultaneously in the solution, solution is heated, make the temperature maintenance of solution at 85～100 ℃; Keeping stirring frequency then is under 40～80 rev/mins the condition, to stir again 30-55 minute, and the solution that constantly will be positioned at the bottom when stirring is derived and added by the top, makes macromolecular substance circulation dissolving in the poly-glycosaminoglycan of carboxylation;
(3) with the can after filtering of the solution after rare joining.
2. preparation method according to claim 1 is characterized in that: describedly densely join process and described rare process of joining is finished in same container.
3. preparation method according to claim 1, it is characterized in that: in the step (3), the solution after described rare joining need be cooled to 70 ℃～80 ℃ after-filtration.
4. preparation method according to claim 1 is characterized in that: this preparation method should be in 1 cubic metres of air the dust number more than 5 microns be no more than under 100 the environment and carry out.
5. the production unit of the poly-glycosaminoglycan medical bio glue of a carboxylation, it is characterized in that: comprise preparing tank, Y-tube, circulation tube and filtration unit, the bottom of described preparing tank is provided with first output tube, described first output tube, described circulation tube and described filtration unit connect three mouths of pipe of described Y-tube respectively, and described circulation tube is connected with the top of described preparing tank away from an end of described Y-tube; Described circulation tube is provided with pneumatic diaphragm pump; One side of described preparing tank is provided with vapour pipe, and described vapour pipe is connected with steam unit away from an end of described preparing tank;
Described preparing tank is used for: carboxylation is gathered the water for injection that glycosaminoglycan is dissolved in 85-100 ℃, finish dense joining and rare process of joining;
Described circulation tube is used for: provide power by pneumatic diaphragm pump, the solution that constantly will be positioned at the preparing tank bottom is derived again by top adding preparing tank;
Described vapour pipe is used for: the steam to solution feeding water for injection, solution is heated, and keeping solution temperature is 85～100 ℃;
Described filtration unit is used for: solution is can after filtering.
6. production unit according to claim 5, it is characterized in that: be provided with stir shaft in the described preparing tank, the length of agitating vane is 60cm on the described stir shaft.
7. production unit according to claim 5, it is characterized in that: described filtration unit comprises second output tube, sanitary pump and millipore filter, described sanitary pump is located on described second output tube, described millipore filter is connected with the mouth of pipe of described Y-tube by described second output tube, and the aperture of described millipore filter is smaller or equal to 0.45 μ m.
8. production unit according to claim 7 is characterized in that: be equipped with diaphragm valve on described first output tube, described circulation tube and described second output tube.
9. according to each described production unit of claim 5-8, it is characterized in that: the sidewall of described preparing tank is provided with thermal insulation layer.
10. production unit according to claim 5, it is characterized in that: also comprise thermometer, the top of described preparing tank is provided with the jack that is used with described thermometer, and an end of described thermometer passes described jack and is located in the preparing tank.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201310244554.1A CN103333348B (en) | 2013-06-19 | 2013-06-19 | Preparation method and production equipment of carboxylated poly(glycosaminoglycan) medical biogum |
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| CN201310244554.1A CN103333348B (en) | 2013-06-19 | 2013-06-19 | Preparation method and production equipment of carboxylated poly(glycosaminoglycan) medical biogum |
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| CN103333348B CN103333348B (en) | 2014-10-22 |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106362621A (en) * | 2016-11-01 | 2017-02-01 | 四川鑫康源生物科技有限公司 | Multifunctional device for stirring and sterilizing health care product |
| CN107158932A (en) * | 2017-07-24 | 2017-09-15 | 中山市中开环保设备制造有限公司 | Organic waste gas treatment device |
| CN110833788A (en) * | 2019-10-22 | 2020-02-25 | 佛山市南海天富科技有限公司 | Saline-alkali solution distribution system |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101036666A (en) * | 2007-04-24 | 2007-09-19 | 山东轻工业学院 | Liquid for preventing conglutination in the surgical and the method for preparing the same |
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2013
- 2013-06-19 CN CN201310244554.1A patent/CN103333348B/en active Active
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101036666A (en) * | 2007-04-24 | 2007-09-19 | 山东轻工业学院 | Liquid for preventing conglutination in the surgical and the method for preparing the same |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106362621A (en) * | 2016-11-01 | 2017-02-01 | 四川鑫康源生物科技有限公司 | Multifunctional device for stirring and sterilizing health care product |
| CN107158932A (en) * | 2017-07-24 | 2017-09-15 | 中山市中开环保设备制造有限公司 | Organic waste gas treatment device |
| CN110833788A (en) * | 2019-10-22 | 2020-02-25 | 佛山市南海天富科技有限公司 | Saline-alkali solution distribution system |
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| CN103333348B (en) | 2014-10-22 |
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