CN103319000B - Enrichment of low temperature resistant autotrophic nitrification microbial agent and its application in wastewater treatment - Google Patents
Enrichment of low temperature resistant autotrophic nitrification microbial agent and its application in wastewater treatment Download PDFInfo
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- 230000001651 autotrophic effect Effects 0.000 title claims abstract description 35
- 238000004065 wastewater treatment Methods 0.000 title claims abstract description 8
- 230000000813 microbial effect Effects 0.000 title 1
- XKMRRTOUMJRJIA-UHFFFAOYSA-N ammonia nh3 Chemical compound N.N XKMRRTOUMJRJIA-UHFFFAOYSA-N 0.000 claims abstract description 42
- 239000010865 sewage Substances 0.000 claims abstract description 37
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 claims abstract description 18
- 229910052799 carbon Inorganic materials 0.000 claims abstract description 18
- 229910052757 nitrogen Inorganic materials 0.000 claims abstract description 16
- 241000894006 Bacteria Species 0.000 claims abstract description 14
- 239000003795 chemical substances by application Substances 0.000 claims abstract description 12
- 239000010802 sludge Substances 0.000 claims abstract description 11
- 230000001546 nitrifying effect Effects 0.000 claims abstract 7
- 238000005273 aeration Methods 0.000 claims description 26
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 21
- 238000000034 method Methods 0.000 claims description 15
- 230000008569 process Effects 0.000 claims description 5
- 229910052760 oxygen Inorganic materials 0.000 claims description 4
- 239000001301 oxygen Substances 0.000 claims description 4
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 claims description 3
- 230000000694 effects Effects 0.000 abstract description 22
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 abstract description 12
- 238000005728 strengthening Methods 0.000 abstract description 12
- 238000012423 maintenance Methods 0.000 abstract description 2
- 230000001580 bacterial effect Effects 0.000 abstract 5
- 239000002068 microbial inoculum Substances 0.000 description 36
- JVMRPSJZNHXORP-UHFFFAOYSA-N ON=O.ON=O.ON=O.N Chemical compound ON=O.ON=O.ON=O.N JVMRPSJZNHXORP-UHFFFAOYSA-N 0.000 description 9
- 230000015556 catabolic process Effects 0.000 description 6
- 238000006731 degradation reaction Methods 0.000 description 6
- 241001453382 Nitrosomonadales Species 0.000 description 3
- 230000008901 benefit Effects 0.000 description 3
- 230000007613 environmental effect Effects 0.000 description 3
- 238000012544 monitoring process Methods 0.000 description 3
- 238000012545 processing Methods 0.000 description 3
- 239000006228 supernatant Substances 0.000 description 3
- 238000012360 testing method Methods 0.000 description 3
- 239000002351 wastewater Substances 0.000 description 3
- 238000009395 breeding Methods 0.000 description 2
- 230000001488 breeding effect Effects 0.000 description 2
- 238000006243 chemical reaction Methods 0.000 description 2
- 239000003153 chemical reaction reagent Substances 0.000 description 2
- 230000003203 everyday effect Effects 0.000 description 2
- 238000005457 optimization Methods 0.000 description 2
- 230000002787 reinforcement Effects 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 238000012163 sequencing technique Methods 0.000 description 2
- 239000007787 solid Substances 0.000 description 2
- 241000233866 Fungi Species 0.000 description 1
- 229910002651 NO3 Inorganic materials 0.000 description 1
- NHNBFGGVMKEFGY-UHFFFAOYSA-N Nitrate Chemical compound [O-][N+]([O-])=O NHNBFGGVMKEFGY-UHFFFAOYSA-N 0.000 description 1
- VMHLLURERBWHNL-UHFFFAOYSA-M Sodium acetate Chemical compound [Na+].CC([O-])=O VMHLLURERBWHNL-UHFFFAOYSA-M 0.000 description 1
- YUWBVKYVJWNVLE-UHFFFAOYSA-N [N].[P] Chemical compound [N].[P] YUWBVKYVJWNVLE-UHFFFAOYSA-N 0.000 description 1
- 150000003863 ammonium salts Chemical class 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 239000002131 composite material Substances 0.000 description 1
- 239000012141 concentrate Substances 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 238000012851 eutrophication Methods 0.000 description 1
- 239000007789 gas Substances 0.000 description 1
- 238000011081 inoculation Methods 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 239000008239 natural water Substances 0.000 description 1
- 230000001590 oxidative effect Effects 0.000 description 1
- 230000010412 perfusion Effects 0.000 description 1
- 230000000737 periodic effect Effects 0.000 description 1
- FGIUAXJPYTZDNR-UHFFFAOYSA-N potassium nitrate Chemical compound [K+].[O-][N+]([O-])=O FGIUAXJPYTZDNR-UHFFFAOYSA-N 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 230000004044 response Effects 0.000 description 1
- 238000005070 sampling Methods 0.000 description 1
- 235000017281 sodium acetate Nutrition 0.000 description 1
- 239000001632 sodium acetate Substances 0.000 description 1
Classifications
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W10/00—Technologies for wastewater treatment
- Y02W10/10—Biological treatment of water, waste water, or sewage
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- Purification Treatments By Anaerobic Or Anaerobic And Aerobic Bacteria Or Animals (AREA)
Abstract
本发明涉及一种耐低温自养硝化菌剂的富集及其在废水处理中的应用。该菌剂在10℃的低温环境下，能够以氨氮为唯一氮源，在好氧环境中将氨氮高效去除；充分实现了硝化细菌在低温条件下的活性维持和持续的硝化速率。该菌剂属于自养菌剂，能够与普通污水处理系统的活性污泥体系实现良好的协同。应用时无需添加有机碳源，菌剂一次性少量投加后很快显现强化效果。将该菌剂应用于实际污水处理，可实现污水处理厂低温条件下的氨氮高效去除，较好解决了传统污水处理厂冬季氨氮达标排放难的问题，具有广阔的应用推广前景。
The invention relates to the enrichment of a low-temperature-resistant autotrophic nitrifying bacteria agent and its application in wastewater treatment. In a low temperature environment of 10°C, the bacterial agent can efficiently remove ammonia nitrogen in an aerobic environment with ammonia nitrogen as the only nitrogen source; it fully realizes the activity maintenance and continuous nitrification rate of nitrifying bacteria under low temperature conditions. The bacterial agent is an autotrophic bacterial agent, which can achieve good synergy with the activated sludge system of the ordinary sewage treatment system. There is no need to add organic carbon sources during application, and the strengthening effect will soon appear after a small amount of bacterial agent is added at one time. The application of the bacterial agent in actual sewage treatment can realize the efficient removal of ammonia nitrogen under low temperature conditions in sewage treatment plants, and better solve the problem of difficult discharge of ammonia nitrogen in traditional sewage treatment plants in winter, and has broad application and promotion prospects.
Description
Technical field
The present invention relates to a kind of enrichment of low temperature resistant Autotrophic nitrification microbial inoculum and the application in wastewater treatment thereof.This microbial inoculum can not only be under cold condition (10 ℃) normal growth and breeding, there is very strong nitrification effect simultaneously.In addition, this microbial inoculum for common sequencing batch activated sludge reactor (SBR) the strengthening effect highly significant under cold condition.
Background technology
Along with the fast development of China's economy, water environmental problems becomes increasingly conspicuous.Wherein, body eutrophication problem directly exceeds standard relevant with the nitrogen phosphorus that enters natural water.At present, for the research majority of town sewage Denitrogenation, concentrate on economy, bioremediation and system efficiently, the ammonia nitrogen water outlet of urban wastewater treatment firm at present can realize qualified discharge substantially.
Water body biological denitrificaion is generally divided into two steps to be completed: (1), by the effect of ammonia oxidizing bacteria (AOB), changes the ammonia-state nitrogen in sewage into nitrite nitrogen; (2) by the effect of nitrite nitrogen oxidizing bacteria (NOB), change nitrite nitrogen into nitric nitrogen.Yet AOB and NOB growth and breeding speed and activity all can significantly reduce under cold condition, the effect of nitrification is very undesirable.China is vast in territory, and most areas are in temperate zone, and changes in temperature are clearly demarcated throughout the year.Therefore, in cold winter, the ammonia nitrogen removal effect of a lot of sewage works is low, and water outlet ammonia nitrogen concentration is difficult to meet emission standard.For fear of the discharge of a large amount of below standard sewage in winter, under cold condition, the research of ammonia nitrogen removal is extremely urgent.
Low temperature test chamber in prior art, in the sewage work of medium above scale, is all equipped with; therefore in the common response device of processing synthetic sewage, all can contain the low temperature resistant nitrifier of minute quantity or trace; only because content is extremely low; be difficult to mass-producing and use, adopt usual way enrichment in a large number at short notice simultaneously.The invention provides a kind of method that makes its enrichment, strengthening.Present method is carried out in accordance with the following steps: the enrichment of (1) Autotrophic nitrification microbial inoculum: under 10 ℃ of cold condition, adopt without the synthetic sewage domestication of the high ammonia nitrogen of organic carbon and the low temperature resistant Autotrophic nitrification flora of enrichment; (2) rapid amplifying of Autotrophic nitrification microbial inoculum: by expanding enrichment reactor volume, increase enrichment system ammonia nitrogen every day bearing capacity, improving aeration rate, further increase quantity and the ratio of low temperature resistant Autotrophic nitrification bacterium; (3) building of the common SBR of strengthening: build a series of identical SBR at cold condition, perfusion common sludge, adopts the synthetic sewage similar with ammonia nitrogen to town sewage COD to move to stable water outlet; (4) microbial inoculum strengthening and effect optimization: according to the nitrification ability of Autotrophic nitrification microbial inoculum, by various dose, each group reaction device is strengthened respectively, optimized strengthening operating parameter simultaneously, obtain best strengthening effect.
First the present invention provides a kind of enriching method with the Autotrophic nitrification microbial inoculum of low temperature tolerance characteristics, it is characterized in that enriching method is:
Select the kind mud in the low-temp reaction device of laboratory to tame and enrichment, be specially:
First stage: to cultivate enrichment without organic carbon, the synthetic sewage of high ammonia nitrogen, aeration rate is 10L/min, aeration time 22h, each cycle 24h, discharge rate 50% in the SBR of 5L; Subordinate phase: to cultivate enrichment without organic carbon, the synthetic sewage of high ammonia nitrogen, aeration rate is 40L/min, aeration time 22h, each cycle 24h, discharge rate 80% in the SBR of 25L; Phase III: to cultivate enrichment without organic carbon, the synthetic sewage of high ammonia nitrogen, aeration rate is 82L/min, aeration time 22h, each cycle 24h, discharge rate 80% in the SBR of 50L;
Obtain having the Autotrophic nitrification microbial inoculum of low temperature tolerance characteristics, wherein said synthetic sewage is containing 115-125mg/LNH
4 +-N, 20-24mg/L PO
4 4--P, containing organic carbon source, NO
2 --N and NO
3 --N, is used NaHCO
3pH as buffer reagent regulation system;
The three phases of enrichment is all realized in 10 ℃ of low hot houses of temperature control;
During enrichment, in system, the scope of dissolved oxygen is 5-11mg/L, and the scope of pH is 7.0-8.0.
The present invention provides a kind of application of above-mentioned Autotrophic nitrification microbial inoculum in wastewater treatment that utilize simultaneously, and (1) selects the SBR of proper volume, initially adds the common sludge of taking from sewage work of about 3000mg/L, moves to stable under 10 ℃ of cold condition; (2) add the low temperature resistant Autotrophic nitrification microbial inoculum of doses; (3) cycle of operation of system is 6h, the DO in Controlling System, temperature and pH value, and whole process is for the COD of water inlet and water outlet, NH simultaneously
4-N, NO
2-N, NH
3-N and DO monitor.
The disposable dosage of wherein said Autotrophic nitrification microbial inoculum is 0.04～0.6g microbial inoculum/L working volume.
It is preferred, and the cycle of operation of system is water inlet 0.5h, anaerobism section 1.0h, aerobic section 3.5h, standing 0.9h, water outlet 0.1h.Intrasystem DO is that 0.3-0.5 (anaerobism section) and 6.0-8.0 (aerobic section), temperature are that 10 ℃, pH value are 7.0-8.0.
The acquisition of the low temperature resistant Autotrophic nitrification microbial inoculum of the present invention, not only makes the ammonia nitrogen water outlet problem of exceeding standard of sewage work in winter be solved, and also has following advantage:
(1) this microbial inoculum nitrification effect under cold condition is very excellent, and the highest nitrated specific degradation rate can reach 8.85mg NH
4-N/ (g SSh), exceeds the nitrification effect of low temperature resistant pure bacterium and microbial inoculum in general document.
(2) this microbial inoculum, completely without organic carbon source, has greater advantage to the town sewage of low COD.Can under cold condition, remove high-ammonia-nitrogen sewage, clearance can reach more than 99% simultaneously.
(3) in strengthening process, to add dosage less for this microbial inoculum, minimum 0.04g microbial inoculum/L working volume that reaches.The time of simultaneously bringing into play strengthening effect is rapid, the most only needs can realize for 2 days 99% above sewage ammonia nitrogen and removes.
(4) adopt the present invention under cold condition, common SBR to be strengthened, without back and forth adding, there is disposable adding and can stablize maintenance good result.
(5) the present invention is applicable to the ammonia nitrogen removal processing of the various waste water of China's winter, has a extensive future, and has good social benefit.
Below in conjunction with embodiment, describe the present invention.Persons of ordinary skill in the art may appreciate that embodiment is only illustrational object, scope of the present invention is not limited with specific implementation method, but is limited by the scope of claim.
Summary of the invention
The present invention aims to provide a kind of have the Autotrophic nitrification microbial inoculum of low temperature tolerance characteristics and the application in wastewater treatment thereof.
Accompanying drawing explanation
Under accompanying drawing 1 cold condition, Autotrophic nitrification microbial inoculum is for common SBR intensifying device schematic diagram, and wherein 1 is pump sump, and 2 is intake pump, and 3 is aeration pump, and 4 is electronic timer, and 5 is gas meter, and 6 is magnetic valve, and 7-10 is respectively 1#-4# reactor.
The degradation curve of the low temperature resistant Autotrophic nitrification microbial inoculum of accompanying drawing 2 to ammonia nitrogen
The low temperature resistant Autotrophic nitrification microbial inoculum of accompanying drawing 3 under various dose for the low-temperature reinforcement effect of common SBR
Concrete embodiment
In embodiment, the method for monitoring and analyzing of various pollutents is with reference to < < water and effluent monitoring analytical procedure > > (the 4th edition, China Environmental Science Press, 2002).Temperature and dissolved oxygen are measured by portable dissolved-oxygen content analyser (YSI550A, USA).Sludge concentration (MLSS) and volatile suspended solid concentration (MLVSS) are according to gravimetric determination.
The various units that use in embodiment, the unified national standard that adopts.
Embodiment 1: the enrichment of low temperature resistant Autotrophic nitrification microbial inoculum under cold condition
The common low temperature test chamber processing of usining is synthesized the excess sludge taking out in the sequencing batch activated sludge reactor (taking from Beijing University's environmental engineering institute low temperature test chamber) of sewage and is tamed and enrichment under cold condition as kind of mud.Because nitrifier is autotrophic bacteria, thereby adopt and to carry out enrichment without organic carbon, high ammonia-nitrogen wastewater, to maximize, obtain Autotrophic nitrification bacterium.The process of whole domestication and enrichment is divided into following three steps:
(1) in the SBR of 5L, to synthesize sewage without the high ammonia nitrogen of organic carbon, cultivate enrichment, aeration rate is 10L/min, aeration time 22h, each cycle 24h, discharge rate 50%;
(2) in the SBR of 25L, to synthesize sewage without the high ammonia nitrogen of organic carbon, cultivate enrichment, aeration rate is 40L/min, aeration time 22h, each cycle 24h, discharge rate 80%;
(3) in the SBR of 50L, to synthesize sewage without the high ammonia nitrogen of organic carbon, cultivate enrichment, aeration rate is 82L/min, aeration time 22h, each cycle 24h, discharge rate 80%;
Collect resulting composite fungus agent, it has outstanding rate of nitrification under cold condition in aerobic environment.
What this wherein used contains 115-125mg/L NH without the synthetic sewage of the high ammonia nitrogen of organic carbon
4 +-N, 20-24mg/L PO
4 4--P, containing organic carbon source, NO
2 --N and NO
3 --N, is used NaHCO
3pH as buffer reagent regulation system.
The design parameter of enrichment three phases and the ammonia nitrogen specific degradation rate finally obtaining are as shown in table 1.
The effect of the cold-resistant Autotrophic nitrification bacterium of table 1 enrichment three phases
The actual degradation time of a is that in system, ammonia nitrogen is removed the required time completely.It is generally less than the time span of each cycle.
B numerical value is taken at the one-period in per latter stage in stage.
The unit of c ammonia nitrogen specific degradation rate is mg NH
4 +-N/ (g suspended solids h).In bracket, the unit of numerical value (rate of nitrification) is mgNH
4 +-N/ (Lh).
As can be seen here, from stage 1 to the stage 3, the Nitrification of low temperature sludge grows steadily, and the specific degradation rate of ammonia nitrogen is from 4.83mg NH
4 +-N/ (g SSh) brings up to 8.85mg NH
4 +-N/ (g SSh), illustrates that in mud, the content of cold-resistant nitrifier significantly increases, and has reached the object of efficiently concentrating.
Embodiment 2: the mensuration of low temperature resistant Autotrophic nitrification microbial inoculum nitrification ability under cold condition
The cold-resistant Autotrophic nitrification microbial inoculum of a certain amount of embodiment 1 stages 3 enrichment is poured in 5L SBR, makes initial MLSS be about 3000mg/L.Add without the synthetic sewage of the high ammonia nitrogen of organic carbon the nitrification ability under its cold condition is measured.Aeration control is 10L/min, makes initial DO be about 4-5mg/L.Every 1h, sample 8mL in 10mL centrifuge tube, and measure intrasystem DO, temperature and pH value.Liquid sample is centrifugal 5min under 8000rpm, gets supernatant liquor and measures NH
4 +-N, NO
2 --N, NO
3 --N, DO and temperature.Intrasystem pH is controlled at 7.0 left and right.
Experimental result as shown in Figure 2.As seen from the figure, under cold condition, in system, the concentration of ammonia nitrogen reduces rapidly from initial 119mg/L, and eliminates completely after 8h.Answer in contrast, nitrite nitrogen and nitrate rise rapidly.Wherein the peak value of nitrite nitrogen concentration appears at 3h, is all converted into afterwards nitric nitrogen.Mineralized nitrogen be the ratio of nitre nitrogen more than 99%, DO rises to 12.0 left and right from 5.0 left and right simultaneously.Explanation thus, under cold condition (envrionment temperature is 10 ℃), the low temperature resistant effect of this microbial inoculum is remarkable, has realized the efficient removal without ammonium salt in organic carbon high ammonia-nitrogen wastewater simultaneously.
Embodiment 3: low temperature resistant Autotrophic nitrification microbial inoculum is for common SBR low-temperature reinforcement effect and optimization
In the low hot house of computer control temperature, (envrionment temperature is 10 ℃) builds 4 identical 5LSBR (1#, 2#, 3# and 4# reactor), as shown in Figure 1.The common sludge of inoculation is taken from sewage work's (on September 20th, 2012), makes 4 initial MLSS of reactor be 3000mg/L.The cycle of operation of system is 6h (water inlet 0.5h, aeration 4h, standing 1h, water outlet 0.5h), and discharges 50% supernatant liquor in the end of term in week.The synthetic sewage using consists of 0.255g/L sodium acetate (200COD), 0.191g/L NH
4cl, 0.044g/L KH
2pO
4, 0.2g/L CaCl
22H
2o, 0.5g/L NaHCO
3.HRT and SRT are respectively 12h and 15 days.In system, DO is 5.8-7.1mg/L.After normally moving 14 days, 4 reactor stable water outlet and approaching, MLSS rises to 3500mg/L simultaneously.
Using R1 as blank group, respectively at 2# to the Autotrophic nitrification microbial inoculum that adds 3g, 0.2g and 0.4g in 4# reactor.Starting mode is the vexed 24-36h that exposes to the sun, afterwards according to original periodic duty.Adding microbial inoculum after 10 days, each cycle aeration time shortens as 3h, and each cycle becomes 5h and continues operation.Object is that observation reduces aeration time and improves ammonia nitrogen loading for the impact of system.
In whole process, select at random every day one-period at standing phase sampling 8mL in 10mL centrifuge tube, and under 8000rpm centrifugal 5min, get supernatant liquor and measure NH
4 +-N, NO
2 --N, NO
3 --N and COD.Gained monitoring result as shown in Figure 3.As seen from the figure, influent ammonia nitrogen maintains 50mg/L left and right.By the prerun of initial two weeks, the water outlet ammonia nitrogen concentration of 4 reactors was all in 20mg/L left and right.After having added the low temperature resistant Autotrophic nitrification microbial inoculum of various dose, 2# to 4# has strengthening effect in various degree.For 2#, after adding 3g microbial inoculum, by the vexed startup of exposing to the sun of 24h, at the second day ammonia nitrogen adding, going out water concentration has significantly reduction, at the 3rd day, has even reached more than 99%.For 3# and 4#, because initial add-on is 0.2g and 0.4g, after vexed the exposing to the sun of 36h, ammonia nitrogen goes out water concentration and is reduced to respectively 10mg/L and 6mg/L, and effect still obviously.After in service, ammonia nitrogen goes out water concentration and declines steadily, adds that after 10 days, to go out water concentration be 4mg/L and 1.5mg/L.When aeration time reduces and improves after ammonia nitrogen loading, 2# still keeps 99% above ammonia nitrogen removal frank.3# and 4# are also uninfluenced, after microbial inoculum adds, within the 15th day, have reached 99% clearance, also maintain preferably afterwards stable.In contrast should, 1# is as blank group, in the cultivation of 35 days, ammonia nitrogen goes out water concentration all more than 15mg/L, illustrates that common sludge of sewage treatment plant has poor effect under cold condition.
As can be seen here, this Autotrophic nitrification microbial inoculum is remarkable to common SBR strengthening effect under cold condition.In addition,, without repeatedly adding microbial inoculum, the system after strengthening has good impact resistance to reducing aeration time and improving ammonia nitrogen loading.
Claims (6)
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| CN111606509A (en) * | 2020-05-20 | 2020-09-01 | 苏州首创嘉净环保科技股份有限公司 | Low-temperature bacterium proportioning culture method for sewage treatment |
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