CN103316722A - Micro fluidic electrophoresis chip integrated with electroosmosis pump - Google Patents
Micro fluidic electrophoresis chip integrated with electroosmosis pump Download PDFInfo
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- CN103316722A CN103316722A CN2013102036605A CN201310203660A CN103316722A CN 103316722 A CN103316722 A CN 103316722A CN 2013102036605 A CN2013102036605 A CN 2013102036605A CN 201310203660 A CN201310203660 A CN 201310203660A CN 103316722 A CN103316722 A CN 103316722A
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Abstract
The invention provides a micro fluidic electrophoresis chip, or rather a micro fluidic electrophoresis chip integrated with an electroosmosis pump. The microfluidic electrophoresis chip raises separation efficiency by introducing an packed column type electroosmosis pump for enhancing electroosmotic flow in a sample feed channel and a separation channel. The micro fluidic electrophoresis chip is composed of a sample pool, the sample feed channel, the separation channel and the packed column type electroosmosis pump. The micro fluidic electrophoresis chip provided by the invention, because of being integrated with the packed column type electroosmosis pump in channels, greatly raises electroosmotic flow efficiency. Accordingly, compared with a normal simple chip, the separation and analysis efficiency of the chip electrophoresis is substantially raised.
Description
Technical field
The present invention relates to a kind of micro-fluidic chip, particularly relate to a kind of micro-fluidic electrophoresis chip.
Background technology
Micro-fluidic chip (microfluidic chip) is the hot fields of current micro-total analysis system (Miniaturized Total Analysis Systems) development.(capillary electrophoresis, the research that develops into microflow control technique CE) makes a breakthrough essential condition is provided Capillary Electrophoresis.1992, Manz and Harrison have delivered capillary electrophoresis separation finished in a first piece of writing at glass micro-fluidic chips paper (Harrison DJ, Manz A, Fan ZH, et al.Capillary electrophoresis and sample injection systems integrated on a planar glass chip.Anal.Chem., 1992,64:1926-1932).After this, the micro-fluidic chip technology especially chip capillary cataphoresis separate and to cause people's extensive concern and further investigation.In September, 1999, first commercialization micro-fluidic chip analyzer device Agilent2100Bioanalyser just is being based on the analysis that the chip capillary cataphoresis principle begins to be applied to nucleic acid and protein.
At present, the channel design of microcurrent controlled capillary tube electrophoresis chip generally is divided into " cross " and intersects and " double T " type cross-over design, often adopts the mode of free solution electrophoresis to carry out the compartment analysis of biological sample.Under this occasion, the chip sample introduction with separate mainly based on the EOF effect of microchannel and realize, so sample introduction and the efficient of separating depend on the EOF characteristic of microchannel.Yet traditional micro-fluid control chip electrophoretic usually depends on the EOF characteristic of single straight channel, often needs very high voltage (thousands of volts and even volt up to ten thousand) to be used for realizing sample introduction and separating for realizing high separating efficiency.Therefore, if can develop a kind of micro-fluidic chip that can under the low voltage condition, obtain than high separating efficiency, be extremely important for the promotion and application of chip electrophoresis technology.
Summary of the invention
At the weak point of above-mentioned micro-fluid control chip electrophoretic technology, the invention provides a kind of micro-fluidic electrophoresis chip, or rather, be a kind of micro-fluidic electrophoresis chip of integrated electroosmotic pump.This electrophoresis chip is to realize the raising of separative efficiency by introduced the filling pillar electroosmotic pump that strengthens EOF in sample intake passage and split tunnel.
Micro-fluidic electrophoresis chip provided by the present invention is made of sample cell, sample intake passage, split tunnel and filling pillar electroosmotic pump.
Micro-fluidic electrophoresis chip provided by the present invention, the material of described electrophoresis chip is glass, quartz, Merlon, polymethyl methacrylate, dimethyl silicone polymer etc., preferred glass is chip material.
Micro-fluidic electrophoresis chip provided by the present invention carries out processing and preparing by existing micro-processing technology, specifically comprises technologies such as photoetching, etching, bonding.For example, the technology of preparation glass-chip is as follows: at first adopt photoetching process to obtain the microchannel figure in conjunction with the method for wet etching at glass substrate, utilize double faced adhesive tape to carry out normal temperature bonding it and another blank glass substrate then, finally be prepared into the micro-fluidic electrophoresis chip of glass material.
Micro-fluidic electrophoresis chip provided by the present invention, described sample intake passage and split tunnel are that " cross " intersects or " double T " type intersects.The length of sample intake passage is 5～20mm, and the length of split tunnel is 30～300mm, and two kinds of width of channel and the degree of depth are consistent, and width is 50～200 μ m, and the degree of depth is 20～100 μ m.
Micro-fluidic electrophoresis chip provided by the present invention has at least one and fills the pillar electroosmotic pump on the described electrophoresis chip, this electroosmotic pump is positioned at the downstream of sample intake passage, also can be distributed in the upstream and downstream of split tunnel simultaneously.
Micro-fluidic electrophoresis chip provided by the present invention, described filling pillar electroosmotic pump is the passage of 30～200 microns of width, the degree of depth 10～100, inside is full of filler.
Micro-fluidic electrophoresis chip provided by the present invention, the width of filling channel and the degree of depth form a cofferdam structure less than sample intake passage and split tunnel in the described filling pillar electroosmotic pump, and filler is limited in the filling channel.
Micro-fluidic electrophoresis chip provided by the present invention, the filler in the described packed column are selected a kind of in silica gel, ion exchange resin, polymer microsphere, the glass microsphere for use, and particle diameter is 50～5000 nanometers, and the surface is electronegative.
When using micro-fluidic chip provided by the invention to carry out the electrophoretic separation of sample, thereby because the expansion effect of EOF can improve sample introduction efficiency and electrophoretic separation efficient significantly.
Description of drawings
Fig. 1. be integrated in the filling pillar electroosmotic pump structural representation of micro-fluidic chip.Wherein, a is electroosmotic pump packed column passage, and b is filler, and c is sample intake passage or split tunnel.
Fig. 2. be integrated with the structural representation of the micro-fluidic electrophoresis chip of three electroosmotic pumps.Wherein, A is sample intake passage; B is split tunnel; C is electroosmotic pump 1; D is electroosmotic pump 2; E electroosmotic pump 3.
Specific embodiments
The following examples will give further instruction to the present invention in conjunction with Figure of description.
The micro-fluidic electrophoresis chip of a kind of integrated electroosmotic pump of embodiment
The present invention proposes a kind of micro-fluidic electrophoresis chip of novel integrated electroosmotic pump, fill pillar electroosmotic pump structure as shown in Figure 1, fill and be full of the electronegative glass microsphere in surface in the pillar passage, particle diameter is about 20 microns.The connector width of filled type passage is 30 μ m and 10 μ m
Micro-fluidic electrophoresis chip as shown in Figure 2, the chip material is glass, sample intake passage A and split tunnel B are that " double T " type intersects, channel width and the degree of depth are respectively 150 μ m and 50 μ m.Wherein, the long 10mm of sample intake passage, the long 40mm of split tunnel.Electroosmotic pump D on the sample intake passage is positioned at 2mm place, downstream, crosspoint, and the electroosmotic pump C on the split tunnel and E are respectively apart from crosspoint 2mm and 35mm.
During the chip electrophoresis experiment, buffer solution injects and is full of whole passages from the buffering liquid pool; Sample to be analyzed adds from injection port, applies certain extraining sampling voltage respectively at sample intake passage and split tunnel two ends, and then sample is along the sample intake passage sample introduction; At this moment, because electroosmotic pump increased the EOF characteristic, so sample introduction efficiency and separative efficiency all obtain significant raising; Switched voltage is to the electrophoretic separation pattern, thereby realizes separation and the detection of sample to be analyzed in split tunnel.
Adopt micro-fluidic electrophoresis chip provided by the invention since in passage integrated filling pillar electroosmotic pump, can improve EOF efficient significantly, the therefore conventional simple chip of contrast, the compartment analysis efficient of this chip electrophoresis obtains significant the raising.The method that the present invention proposes can be widely used in the efficient compartment analysis of nucleic acid, protein and materials such as amino acid, ion.
Claims (10)
1. the micro-fluidic electrophoresis chip of an integrated electroosmotic pump is characterized in that, this electrophoresis chip is made of sample cell, sample intake passage, split tunnel and filling pillar electroosmotic pump.
2. according to the micro-fluidic electrophoresis chip of the described a kind of integrated electroosmotic pump of claim 1, it is characterized in that the material of described electrophoresis chip is glass, quartz, Merlon, polymethyl methacrylate, dimethyl silicone polymer etc.
3. according to the micro-fluidic electrophoresis chip of the described a kind of integrated electroosmotic pump of claim 1, it is characterized in that described electrophoresis chip carries out processing and preparing by existing micro-processing technology.
4. according to the micro-fluidic electrophoresis chip of the described a kind of integrated electroosmotic pump of claim 1, it is characterized in that described sample intake passage and split tunnel are that " cross " intersects or " double T " type intersects.
5. according to the micro-fluidic electrophoresis chip of the described a kind of integrated electroosmotic pump of claim 1, it is characterized in that the length of sample intake passage is 5～20mm, the length of split tunnel is 30～300mm, two kinds of width of channel and the degree of depth are consistent, and width is 50～200 μ m, and the degree of depth is 20～100 μ m.
6. according to the micro-fluidic electrophoresis chip of the described a kind of integrated electroosmotic pump of claim 1, it is characterized in that, have at least one on the described electrophoresis chip and fill the pillar electroosmotic pump, this electroosmotic pump is positioned at the downstream of sample intake passage, also can be distributed in the upstream and downstream of split tunnel simultaneously.
7. according to the micro-fluidic electrophoresis chip of the described a kind of integrated electroosmotic pump of claim 1, it is characterized in that described filling pillar electroosmotic pump is the passage of 30～200 microns of width, the degree of depth 10～100, inside is full of filler.
8. according to the micro-fluidic electrophoresis chip of the described a kind of integrated electroosmotic pump of claim 1, it is characterized in that, the width of filling channel and the degree of depth form a cofferdam structure less than sample intake passage and split tunnel in the described filling pillar electroosmotic pump, and filler is limited in the filling channel.
9. according to the micro-fluidic electrophoresis chip of the described a kind of integrated electroosmotic pump of claim 1, it is characterized in that, filler in the described packed column is selected a kind of in silica gel, ion exchange resin, polymer microsphere, the glass microsphere for use, and particle diameter is 50～5000 nanometers, and the surface is electronegative.
10. according to the micro-fluidic electrophoresis chip of the described a kind of integrated electroosmotic pump of claim 1, it is characterized in that, when using micro-fluidic chip provided by the invention to carry out the electrophoretic separation of sample, thereby because the expansion effect of EOF can improve sample introduction efficiency and electrophoretic separation efficient significantly.
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| CN2013102036605A CN103316722A (en) | 2013-05-27 | 2013-05-27 | Micro fluidic electrophoresis chip integrated with electroosmosis pump |
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| CN2013102036605A CN103316722A (en) | 2013-05-27 | 2013-05-27 | Micro fluidic electrophoresis chip integrated with electroosmosis pump |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103926190A (en) * | 2014-05-08 | 2014-07-16 | 齐鲁工业大学 | Automatic single cell analysis method based on microfluidic system |
| CN110479391A (en) * | 2019-08-05 | 2019-11-22 | 湖北医药学院 | A kind of low-voltage high-performance electric osmose Micropump chip based on solid-state track etching nano-pore |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1419954A (en) * | 2001-11-15 | 2003-05-28 | 中国科学院大连化学物理研究所 | Chip micro flow electroosmosis pump |
-
2013
- 2013-05-27 CN CN2013102036605A patent/CN103316722A/en active Pending
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1419954A (en) * | 2001-11-15 | 2003-05-28 | 中国科学院大连化学物理研究所 | Chip micro flow electroosmosis pump |
Non-Patent Citations (2)
| Title |
|---|
| TRUST T. RAZUNGUZWA ET AL.: "Fabrication and Characterization of a Fritless Microfabricated Electroosmotic Pump with Reduced pH Dependence", 《ANALYTICAL CHEMISTRY》, vol. 76, no. 5, 22 January 2004 (2004-01-22), pages 1336 - 1341, XP001196703, DOI: doi:10.1021/ac034956e * |
| 李清岭等: "微流体驱动与控制技术", 《化学进展》, vol. 20, no. 9, 30 September 2008 (2008-09-30), pages 1406 - 1414 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103926190A (en) * | 2014-05-08 | 2014-07-16 | 齐鲁工业大学 | Automatic single cell analysis method based on microfluidic system |
| CN110479391A (en) * | 2019-08-05 | 2019-11-22 | 湖北医药学院 | A kind of low-voltage high-performance electric osmose Micropump chip based on solid-state track etching nano-pore |
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Application publication date: 20130925 |