CN107670736A - A kind of micro-fluidic electrophoresis chip - Google Patents
A kind of micro-fluidic electrophoresis chip Download PDFInfo
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- CN107670736A CN107670736A CN201710904135.4A CN201710904135A CN107670736A CN 107670736 A CN107670736 A CN 107670736A CN 201710904135 A CN201710904135 A CN 201710904135A CN 107670736 A CN107670736 A CN 107670736A
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D57/00—Separation, other than separation of solids, not fully covered by a single other group or subclass, e.g. B03C
- B01D57/02—Separation, other than separation of solids, not fully covered by a single other group or subclass, e.g. B03C by electrophoresis
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
- B01L3/502707—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by the manufacture of the container or its components
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
- B01L3/50273—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by the means or forces applied to move the fluids
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
- B01L3/502761—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip specially adapted for handling suspended solids or molecules independently from the bulk fluid flow, e.g. for trapping or sorting beads, for physically stretching molecules
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
- B01L2200/02—Adapting objects or devices to another
- B01L2200/026—Fluid interfacing between devices or objects, e.g. connectors, inlet details
- B01L2200/027—Fluid interfacing between devices or objects, e.g. connectors, inlet details for microfluidic devices
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/08—Geometry, shape and general structure
- B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/12—Specific details about materials
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2400/00—Moving or stopping fluids
- B01L2400/04—Moving fluids with specific forces or mechanical means
- B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
- B01L2400/0415—Moving fluids with specific forces or mechanical means specific forces electrical forces, e.g. electrokinetic
- B01L2400/0421—Moving fluids with specific forces or mechanical means specific forces electrical forces, e.g. electrokinetic electrophoretic flow
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- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Dispersion Chemistry (AREA)
- Analytical Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Hematology (AREA)
- Clinical Laboratory Science (AREA)
- Life Sciences & Earth Sciences (AREA)
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- Environmental & Geological Engineering (AREA)
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- Electrostatic Separation (AREA)
Abstract
The present invention provides a kind of micro-fluidic electrophoresis chip, and the electrophoresis chip is to realize the raising of separative efficiency by introducing the array MCA of enhancing EOF in sample intake passage and split tunnel.Micro-fluidic electrophoresis chip provided by the present invention, there is at least one parallel micro-channel array structure on described electrophoresis chip, the structure is located at the downstream of sample intake passage, can also be distributed in the upstream and downstream of split tunnel simultaneously.Using micro-fluidic chip provided by the invention carry out sample electrophoretic separation when, due to EOF expansion effect so as to significantly increase sample introduction efficiency and electrophoretic separation efficiency.
Description
Technical field
The present invention relates to a kind of micro-fluidic chip, more particularly to a kind of micro-fluidic electrophoresis chip.
Background technology
Micro-fluidic chip (microfluidic chip) is current micro-total analysis system (Miniaturized Total
Analysis Systems) development hot fields.The hair of Capillary Electrophoresis (capillary electrophoresis, CE)
Open up and provide essential condition for the Remarkable Progress On Electric Artificial of microflow control technique.1992, Manz and Harrison delivered a first piece in glass
Paper (Harrison DJ, Manz A, the Fan ZH, et of capillary electrophoresis separation are completed on glass micro-fluidic chip
al.Capillary electrophoresis and sample injection systems integrated on a
Planar glass chip.Anal.Chem., 1992,64：1926-1932).Hereafter, microfluidic chip technology especially core
Piece capillary electrophoresis separation causes extensive concern and the further investigation of people.In September, 1999, the micro-fluidic core of First commercialization
Piece analytical instrument Agilent2100Bioanalyser is based on chip capillary cataphoresis principle and starts to be applied to nucleic acid and egg
The analysis of white matter.
At present, the channel design of microcurrent controlled capillary tube electrophoresis chip is generally divided into " cross " intersection and " double T " types, which intersect, to be set
Meter, the separation analysis of biological sample is often carried out by the way of free solution electrophoresis.In this case, chip sample introduction and
Separation is based primarily upon the EOF effect of microchannel and realized, therefore sample introduction and the efficiency of separation depend on the electric osmose of microchannel
Properties of flow.However, traditional micro-fluid control chip electrophoretic frequently relies on the electric osmose properties of flow of single straight channel, it is high to realize
Separative efficiency generally requires very high voltage (several thousand volts or even volt up to ten thousand) and is used to realize sample introduction and separation.Therefore, if
A kind of micro-fluidic chip that can be obtained under the conditions of low voltage compared with high separating efficiency can be developed, for chip electrophoresis technology
Promotion and application be extremely important.
The content of the invention
For the weak point of above-mentioned micro-fluid control chip electrophoretic technology, the present invention provides a kind of micro-fluidic electrophoresis chip,
The electrophoresis chip is realized by introducing the array MCA of enhancing EOF in sample intake passage and split tunnel
The raising of separative efficiency.
Micro-fluidic electrophoresis chip provided by the present invention, the material of described electrophoresis chip is glass, quartz, poly- carbonic acid
Ester, polymethyl methacrylate, dimethyl silicone polymer etc..The use of the preferable glass of electric osmose properties of flow is preferentially micro-fluidic electricity
The material of swimming chip.
Micro-fluidic electrophoresis chip provided by the present invention, described electrophoresis chip are entered by existing micro-processing technology
Prepared by row processing, specifically include the techniques such as photoetching, etching, bonding.For example, the technique for preparing glass-chip is as follows：Adopt first
Microchannel figure is obtained on a glass substrate with the method for photoetching process combination wet etching, then by it and another blank glass
Substrate carries out high temperature bonding under the conditions of 500~600 DEG C, is finally prepared into glass base micro-fluidic electrophoresis chip.
Micro-fluidic electrophoresis chip provided by the present invention, described sample cell include sample inlet pool and waste liquid pool, a diameter of 1~
5mm.Described sample intake passage and split tunnel are that " cross " intersects or " double T " types intersect.
Micro-fluidic electrophoresis chip provided by the present invention, the length of sample intake passage is 5~20mm, and the length of split tunnel is 30
~300mm, the width and depth of two kinds of passages are consistent, and width is 50~200 μm, and depth is 20~100 μm.
Micro-fluidic electrophoresis chip provided by the present invention, have on described electrophoresis chip at least one parallel fine
Channel array structure, the structure are located at the downstream of sample intake passage, can also be distributed in the upstream and downstream of split tunnel simultaneously.It is described
Passage upstream and downstream, be for the cross-point locations of sample intake passage and split tunnel.
When carrying out the electrophoretic separation of sample using micro-fluidic chip provided by the invention, due to the expansion effect of EOF
So as to significantly increase sample introduction efficiency and electrophoretic separation efficiency.
The structural representation of Fig. 1 arrays microchannel.
Fig. 2 contain the structural representation of the micro-fluidic electrophoresis chip of an array MCA.Wherein, A is sample introduction
Pond；B is sample intake passage；C is sample waste pond；D is buffer pool；E is split tunnel；F is waste liquid pool；G is that array is micro- logical
Road.
Fig. 3 contain the structural representation of the micro-fluidic electrophoresis chip of three array MCAs.Wherein, A ' is sample introduction
Pond；B ' is sample intake passage；C ' is sample waste pond；D ' is buffer pool；E ' is split tunnel；F ' is waste liquid pool；G ' is array
Microchannel 1；H ' is that array microchannel 2, I ' is array microchannel 3.
The following examples will be further described with reference to Figure of description to the present invention.
A kind of " cross " the chiasma type micro-fluidic electrophoresis chip of embodiment 1
For a kind of " cross " chiasma type micro-fluidic electrophoresis chip as shown in Fig. 2 chip material is glass, sample intake passage B and separation are logical
Road E intersects, and channel width and depth are respectively 150 μm and 50 μm.Wherein, the long 10mm of sample intake passage, the long 40mm of split tunnel.
G on sample intake passage is array microchannel, and at the 2mm of crosspoint downstream, its structure is as shown in figure 1, be 10 μ by 8 width
M, depth is 5 μm, formed at intervals of 10 μm of array microchannels.
When chip electrophoresis is tested, buffer solution injects from buffer pool D and is full of whole passages；Sample to be analysed is from sample introduction
Mouth A is added, and applies certain extraining sampling voltage respectively in sample intake passage and split tunnel both ends, then sample leads to along sample introduction
Road sample introduction；Now, because array microchannel increases electric osmose properties of flow, therefore sample introduction efficiency greatly improves；Switch voltage to electricity
Swimming clastotype, so as to realize separation and detection of the sample to be analysed in split tunnel.
A kind of " double T " the chiasma types micro-fluidic electrophoresis chips of embodiment 2
It is a kind of that " for double T " chiasma types micro-fluidic electrophoresis chips as shown in figure 3, chip material is glass, sample intake passage B ' and separation are logical
Road E ' is " double T " intersect, i.e. two passages have certain sample introduction distance between intersecting.Channel width and depth be respectively 150 μm and
50μm.Wherein, the long 10mm of sample intake passage, the long 40mm of split tunnel, two intersections are 200 μm.G ' on sample intake passage and
H ' and I ' on split tunnel are array microchannel, and G ' is located at the 2mm of crosspoint downstream, and H ' is located at the 2mm of crosspoint upstream,
I ' is located at crosspoint downstream 25mm.Its structure as shown in figure 1, be 10 μm by 8 width, depth be 5 μm, at intervals of 10 μm
Array microchannel is formed.
When chip electrophoresis is tested, buffer solution injects from buffer pool D ' and is full of whole passages；Sample to be analysed is from sample introduction
Mouth A ' is added, and applies certain extraining sampling voltage respectively in sample intake passage and split tunnel both ends, then sample leads to along sample introduction
Road sample introduction；Now, because array microchannel increases electric osmose properties of flow, therefore sample introduction efficiency and separative efficiency obtain significantly
Raising；Switch voltage to pattern is separated by electrophoresis, so as to realize separation and detection of the sample to be analysed in split tunnel.
, can be with larger due to being integrated with array microchannel in the channel using micro-fluidic electrophoresis chip provided by the invention
Ground improves EOF efficiency, therefore contrasts conventional simple chip, and the separation analysis efficiency of the chip electrophoresis, which obtains, significantly to be carried
It is high.Method proposed by the present invention can be widely applied to efficiently separating point for the material such as nucleic acid, protein and amino acid, ion
Analysis.
Claims (9)
1. a kind of micro-fluidic electrophoresis chip, it is characterised in that the electrophoresis chip is by sample cell, sample intake passage and split tunnel structure
Into.
2. according to a kind of micro-fluidic electrophoresis chip described in claim 1, it is characterised in that the material of described electrophoresis chip
For glass, quartz, makrolon, polymethyl methacrylate, dimethyl silicone polymer etc..
3. according to a kind of micro-fluidic electrophoresis chip described in claim 1, it is characterised in that described electrophoresis chip is to pass through
Existing micro-processing technology is processed preparation, specifically includes the techniques such as photoetching, etching, bonding.
4. according to a kind of micro-fluidic electrophoresis chip described in claim 1, it is characterised in that described sample cell includes sample introduction
Pond and waste liquid pool, a diameter of 1~5mm.
5. according to a kind of micro-fluidic electrophoresis chip described in claim 1, it is characterised in that described sample intake passage and separation
Passage is that " cross " intersects or " double T " types intersect.
6. according to a kind of micro-fluidic electrophoresis chip described in claim 1, it is characterised in that the length of described sample intake passage
For 5~20mm, the length of split tunnel is 30~300mm, and the width and depth of two kinds of passages are consistent, width is 50~
200 μm, depth is 20~100 μm.
7. according to a kind of micro-fluidic electrophoresis chip described in claim 1, it is characterised in that have on described electrophoresis chip
At least one parallel micro-channel array structure, the structure are located at the downstream of sample intake passage, can also be distributed in separation simultaneously
The upstream and downstream of passage.
8. according to a kind of micro-fluidic electrophoresis chip described in claim 1 and 7, it is characterised in that above and below described passage
Trip, is for the cross-point locations of sample intake passage and split tunnel.
9. according to a kind of micro-fluidic electrophoresis chip described in claim 1, it is characterised in that use miniflow provided by the invention
Control chip carry out sample electrophoretic separation when, due to EOF expansion effect so as to significantly increase sample introduction efficiency and
Efficiency is separated by electrophoresis.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201710904135.4A CN107670736A (en) | 2017-09-29 | 2017-09-29 | A kind of micro-fluidic electrophoresis chip |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201710904135.4A CN107670736A (en) | 2017-09-29 | 2017-09-29 | A kind of micro-fluidic electrophoresis chip |
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| CN107670736A true CN107670736A (en) | 2018-02-09 |
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| CN201710904135.4A Withdrawn CN107670736A (en) | 2017-09-29 | 2017-09-29 | A kind of micro-fluidic electrophoresis chip |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110596223A (en) * | 2019-09-19 | 2019-12-20 | 电子科技大学 | Micro-fluidic enrichment device and method based on electroosmosis induced pressure flow |
-
2017
- 2017-09-29 CN CN201710904135.4A patent/CN107670736A/en not_active Withdrawn
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110596223A (en) * | 2019-09-19 | 2019-12-20 | 电子科技大学 | Micro-fluidic enrichment device and method based on electroosmosis induced pressure flow |
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Application publication date: 20180209 |