CN103310684A - Detachable immobilized enzyme or immobilized cell model teaching aid - Google Patents
Detachable immobilized enzyme or immobilized cell model teaching aid Download PDFInfo
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- CN103310684A CN103310684A CN2013102725627A CN201310272562A CN103310684A CN 103310684 A CN103310684 A CN 103310684A CN 2013102725627 A CN2013102725627 A CN 2013102725627A CN 201310272562 A CN201310272562 A CN 201310272562A CN 103310684 A CN103310684 A CN 103310684A
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Abstract
The invention discloses a detachable immobilized enzyme or immobilized cell model teaching aid. The detachable immobilized enzyme or immobilized cell model teaching aid comprises a disc-shaped basin body, a cover body movably arranged above the basin body, a first connecting body, a second connecting body, a third connecting body and a fourth connecting body. The top face of the cover body is a hollow-out screen, the first connecting body is a sphere, the second connecting body is a sphere provided with a connecting rod at the bottom, the third connecting body is a sphere provided with an inverted T-shaped connecting body at the bottom, and the fourth connecting body is an annular bead string provided with connecting rods at the bottoms of node beads. The detachable immobilized enzyme or immobilized cell model teaching aid is simple in structure and can lively and visually show various immobilized modes of enzymes or cells.
Description
Technical field
The present invention relates to the teaching aid field, relate in particular to a kind of reassembling type immobilised enzymes or immobilized cell model teaching aid.
Background technology
Microorganism can be regarded the parcel of plurality of enzymes as, industrial fermentation is the process of rationally controlling and utilize microbial enzyme, therefore, enzyme can be extracted from microbial cell, itself and substrate-function are manufactured a product, also can the enzyme that extract is fixing with solid support (becoming carrier), make it become water insoluble or be difficult for scattering and reused microbial catalyst, utilize it and substrate-function to manufacture a product.Loose enzyme (cell) is used for commercial production, can be called resolvase (cell), and fixing enzyme is called immobilised enzymes, and fixing microbial cell is called immobilized cell.The advantage that immobilised enzymes (cell) has is: 1, immobilised enzymes (cell) can be reused, and has improved greatly production efficiency; 2, the aftertreatment ratios such as the separation of immobilised enzymes (cell) product, purification are easier to; 3, immobilised enzymes (cell) has generally all been made spheric grain or laminar, makes the production technology simplified control of product, is easy to mechanization and robotization, and equipment and equipment are also than being easier to; 4, can to make enzyme activity very high for immobilised enzymes (cell), and the performance of antiacid, alkali, temperature variation is high, and enzyme activity is stable; 5, the relative product of immobilised enzymes is more single, undesirable product still less, the production operation condition is more easy to control, and immobilized cell does not need the extraction of enzyme, has reduced loss and the operation of enzyme activity.
In teaching field, generally all adopt picture to explain the immobilization way of enzyme (cell), but picture is the architectural feature that can not well embody immobilised enzymes (cell) of two dimension and the immobilization way of enzyme (cell), therefore, needs a kind of instrument of new aided education.
Summary of the invention
The purpose of this invention is to provide a kind of simple in structure, dismountable immobilised enzymes or immobilized cell model teaching aid.
To achieve the above object of the invention, the technical solution used in the present invention is: a kind of reassembling type immobilised enzymes or immobilized cell model teaching aid, it comprises discoidal basin body, be movably arranged on the lid of described basin body top, the first connector, the second connector, the 3rd connector, the 4th connector, described lid end face is the hollow out net, described the first connector is spheroid, described the second connector is the spheroid that the bottom is provided with connecting link, described the 3rd connector is the spheroid that the bottom is provided with the connector of inverted T-shaped, and described the 4th connector is that the bottom is provided with the annular pearl string that the spheroid of connecting link is formed by connecting.
Preferred technical scheme, described basin bottom is hemispheric hollow out net, described lid end face hollow out net is semisphere.
Preferred technical scheme, described basin body lateral surface is provided with external thread, and described lid medial surface is provided with internal thread.
Preferred technical scheme, described the first connector absorption is arranged in the described basin body.
Preferred technical scheme, described the 3rd connector absorption is arranged on described lid lateral surface.
Preferred technical scheme, described lid lateral surface is provided with the bayonet socket that matches with described connecting link.
Further technical scheme, described the second connector is around being arranged on described lid lateral surface.
Further technical scheme, described the 4th connector is around being arranged on described lid lateral surface.
Enzyme is fixed with different methods of operating with different carriers, according to immobilized dominant mechanism, generally be divided into 4 classes: 1, adsorption of immobilization, according to the principle that positive and negative charge is inhaled mutually, enzyme or cell are adsorbed on carrier surface and are fixed; 2, embedded immobilization, macromolecular organic or inorganic polymkeric substance wraps up enzyme or cell, be fixed in its gel; 3, covalent immobilization, enzyme or cell and carrier are fixed by covalent bond; 4, crosslinked immobilization, under the effect of bifunctional group crosslinking chemical, being cross-linked with each other with compound group on the carrier is reticulate texture and is fixed between the compound group on enzyme molecule or the cell.
In the technique scheme, according to immobilized dominant mechanism, be divided into 4 class formations and cooperate: 1, adsorption of immobilization, basin body and lid represent carrier together, and the 3rd connector represents enzyme or cell; 2, embedded immobilization, basin body and lid represent carrier together, and the first connector represents enzyme or cell; 3, covalent immobilization, basin body and lid represent carrier together, and the second connector represents enzyme or cell; 4, crosslinked immobilization, basin body and lid represent carrier together, and the 4th connector represents enzyme or cell.
Because technique scheme is used, the present invention compared with prior art has following advantages:
The present invention is disassembly and assembly structure, simple in structure, easy to use, the immobilization way that can not only embody structural form, enzyme or the cell of immobilised enzymes or cell can also improve operator's manipulative ability, deepen the operator to the memory of immobilised enzymes or immobilized cell structure.
Description of drawings
Fig. 1 is exploded view of the present invention;
Fig. 2 is schematic diagram of the present invention.
Wherein: 1, basin body; 2, lid; 3, the first connector; 4, the second connector; 5, the 3rd connector; 6, the 4th connector; 7, bayonet socket; 8, connecting link; 9, connector.
Embodiment
The invention will be further described below in conjunction with drawings and Examples:
Embodiment one:
As shown in Figure 1, 2, a kind of reassembling type immobilised enzymes or immobilized cell model teaching aid, it comprises discoidal basin body 1, be movably arranged on the lid 2 of basin body 1 top, the first connector 3, the second connector 4, the 3rd connector 5, the 4th connector 6, lid 2 end faces are the hollow out net, the first connector 3 is spheroid, the second connector 4 is provided with the spheroid of connecting link 8 for the bottom, the 3rd connector 5 is provided with the spheroid of the connector 9 of inverted T-shaped for the bottom, the 4th connector 6 is provided with the annular pearl string that the spheroid of connecting link 8 is formed by connecting for the bottom.
Basin body 1 bottom is hemispheric hollow out net, and described lid 2 end face hollow out nets are semisphere.
Basin body 1 lateral surface is provided with external thread, and lid 2 medial surfaces are provided with internal thread.
The material of basin body 1, lid 2 is stainless steel.
Be provided with magnet on the first connector 3, the 3rd connector 5 bottom connectors 9 are provided with magnet.
The first connector 3 is provided with 20, and the second connector 4 is provided with 22, and the 3rd connector 5 is provided with 15.
During use, be divided into 4 class formations and cooperate:
1, adsorption of immobilization, according to the principle that positive and negative charge is inhaled mutually, enzyme or cell are adsorbed on carrier surface and are fixed, and basin body 1 and lid 2 represent carrier together, and the 3rd connector 5 represents enzyme or cell, and the 3rd connector 5 is adsorbed on lid 2 outside surfaces;
2, embedded immobilization, macromolecular organic or inorganic polymkeric substance wraps up enzyme or cell, be fixed in its gel, and basin body 1 and lid 2 represent carrier together, and the first connector 3 represents enzyme or cell, the first connector 3 is placed be adsorbed in the basin body 1;
3, covalent immobilization, enzyme or cell and carrier are fixed by covalent bond, and basin body 1 and lid 2 represent carrier together, and the second connector 4 represents enzyme or cell, the second connector 4 is inserted in the bayonet socket 7 of lid 2 lateral surfaces;
4, crosslinked immobilization, between the compound group on enzyme molecule or the cell under the effect of bifunctional group crosslinking chemical, be cross-linked with each other with compound group on the carrier and be reticulate texture and be fixed, basin body 1 and lid 2 represent carrier together, the 4th connector 6 represents enzyme or cell, the 4th connector 6 is inserted in the bayonet socket 7 of lid 2 lateral surfaces.
Claims (8)
1. a reassembling type immobilised enzymes or immobilized cell model teaching aid, it is characterized in that: it comprises discoidal basin body, is movably arranged on lid, the first connector, the second connector, the 3rd connector, the 4th connector of described basin body top, described lid end face is the hollow out net, described the first connector is spheroid, described the second connector is the spheroid that the bottom is provided with connecting link, described the 3rd connector is the spheroid that the bottom is provided with the connector of inverted T-shaped, and described the 4th connector is that the bottom is provided with the annular pearl string that the spheroid of connecting link is formed by connecting.
2. a kind of reassembling type immobilised enzymes according to claim 1 or immobilized cell model teaching aid, it is characterized in that: described basin bottom is hemispheric hollow out net, described lid end face hollow out net is semisphere.
3. a kind of reassembling type immobilised enzymes according to claim 1 or immobilized cell model teaching aid, it is characterized in that: described basin body lateral surface is provided with external thread, and described lid medial surface is provided with internal thread.
4. a kind of reassembling type immobilised enzymes according to claim 1 or immobilized cell model teaching aid is characterized in that: be provided with magnet on described the first connector, described the first connector absorption is arranged in the described basin body.
5. a kind of reassembling type immobilised enzymes according to claim 1 or immobilized cell model teaching aid is characterized in that: described the 3rd connector bottom is provided with magnet, and described the 3rd connector absorption is arranged on described lid lateral surface.
6. a kind of reassembling type immobilised enzymes according to claim 1 or immobilized cell model teaching aid, it is characterized in that: described lid lateral surface is provided with the bayonet socket that matches with described connecting link.
7. it is characterized in that according to claim 1 or 6 described a kind of reassembling type immobilised enzymes or immobilized cell model teaching aids: described the second connector is around being arranged on described lid lateral surface.
8. it is characterized in that according to claim 1 or 6 described a kind of reassembling type immobilised enzymes or immobilized cell model teaching aids: described the 4th connector is around being arranged on described lid lateral surface.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310272562.7A CN103310684B (en) | 2013-07-01 | 2013-07-01 | Detachable immobilized enzyme or immobilized cell model teaching aid |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310272562.7A CN103310684B (en) | 2013-07-01 | 2013-07-01 | Detachable immobilized enzyme or immobilized cell model teaching aid |
Publications (2)
| Publication Number | Publication Date |
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| CN103310684A true CN103310684A (en) | 2013-09-18 |
| CN103310684B CN103310684B (en) | 2015-04-22 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201310272562.7A Expired - Fee Related CN103310684B (en) | 2013-07-01 | 2013-07-01 | Detachable immobilized enzyme or immobilized cell model teaching aid |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108389492A (en) * | 2018-04-27 | 2018-08-10 | 佛山拾念科技有限公司 | A kind of dismountable bioconversion medium plant cell mold |
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| US20060172412A1 (en) * | 2005-02-02 | 2006-08-03 | Coletica | Support device for cells culture |
| CN201829119U (en) * | 2010-10-28 | 2011-05-11 | 张馨心 | Plant cell structure model |
| CN202662188U (en) * | 2012-06-29 | 2013-01-09 | 苏州大学 | Phage model teaching aid |
| CN102938225A (en) * | 2012-09-06 | 2013-02-20 | 苏州大学 | Adenosine triphosphate (ATP) synthase model training aid |
| CN202749004U (en) * | 2012-09-06 | 2013-02-20 | 苏州大学 | Rhizopus model teaching aid |
| CN202795826U (en) * | 2012-06-04 | 2013-03-13 | 辽宁石油化工大学 | Yeast reproduction process teaching model |
| CN202795824U (en) * | 2012-06-04 | 2013-03-13 | 辽宁石油化工大学 | Bacterium cellular structure teaching demonstration model |
| CN202795867U (en) * | 2012-09-25 | 2013-03-13 | 李依韦 | Cell immobilization device for teaching |
| CN203455971U (en) * | 2013-07-01 | 2014-02-26 | 苏州大学 | Detachable immobilized enzyme or immobilized cell model teaching aid |
-
2013
- 2013-07-01 CN CN201310272562.7A patent/CN103310684B/en not_active Expired - Fee Related
Patent Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20060172412A1 (en) * | 2005-02-02 | 2006-08-03 | Coletica | Support device for cells culture |
| CN201829119U (en) * | 2010-10-28 | 2011-05-11 | 张馨心 | Plant cell structure model |
| CN202795826U (en) * | 2012-06-04 | 2013-03-13 | 辽宁石油化工大学 | Yeast reproduction process teaching model |
| CN202795824U (en) * | 2012-06-04 | 2013-03-13 | 辽宁石油化工大学 | Bacterium cellular structure teaching demonstration model |
| CN202662188U (en) * | 2012-06-29 | 2013-01-09 | 苏州大学 | Phage model teaching aid |
| CN102938225A (en) * | 2012-09-06 | 2013-02-20 | 苏州大学 | Adenosine triphosphate (ATP) synthase model training aid |
| CN202749004U (en) * | 2012-09-06 | 2013-02-20 | 苏州大学 | Rhizopus model teaching aid |
| CN202795867U (en) * | 2012-09-25 | 2013-03-13 | 李依韦 | Cell immobilization device for teaching |
| CN203455971U (en) * | 2013-07-01 | 2014-02-26 | 苏州大学 | Detachable immobilized enzyme or immobilized cell model teaching aid |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108389492A (en) * | 2018-04-27 | 2018-08-10 | 佛山拾念科技有限公司 | A kind of dismountable bioconversion medium plant cell mold |
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| CN103310684B (en) | 2015-04-22 |
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