CN103305569A - Method for producing pullulan by aureobasidium pullulans AY82 by using xylose - Google Patents
Method for producing pullulan by aureobasidium pullulans AY82 by using xylose Download PDFInfo
- Publication number
- CN103305569A CN103305569A CN2013101699150A CN201310169915A CN103305569A CN 103305569 A CN103305569 A CN 103305569A CN 2013101699150 A CN2013101699150 A CN 2013101699150A CN 201310169915 A CN201310169915 A CN 201310169915A CN 103305569 A CN103305569 A CN 103305569A
- Authority
- CN
- China
- Prior art keywords
- xylose
- fermentation
- aureobasidium pullulans
- wood sugar
- pulullan
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Images
Abstract
The invention discloses a method for producing pullulan by aureobasidium pullulans AY82 by using xylose. A mutant strain which has high yield of the pullulan and secretes little pigment is obtained by performing ultraviolet mutagenesis on the aureobasidium pullulans serving as an original strain. The aureobasidium pullulans mutant strain AY82 is preserved in the China general microbiological culture collection center (CGMCC) in January, 18th in 2013 with the preservation number of CGMCC No.7154. The strain can produce the pullulan by using the xylose by fermentation; the yield of the pullulan is higher; after a xylose fermentation culture medium shake flask is cultivated for seven days, the yield of the pullulan is up to 16.61 g/L; and after a fermentation pot of 5 liters is cultivated for seven days, the yield is up to 16.23 g/L. A research shows that the pullulan can be produced by the aureobasidium pullulans by using the xylose; a new concept can be supplied to rational utilization of the xylose; and the additional value of the xylose can be effectively increased.
Description
Technical field:
The invention belongs to the microbial fermentation technology field, be specifically related to a strain Aureobasidium pullulans bacterial strain and utilize the method for xylose production pulullan.
Background technology:
Pulullan is the outer homopolysaccharide of a kind of unbranched born of the same parents that Aureobasidium pullulans (Aureobasidium Pullulan) produces, and it is constantly to be polymerized by the trisaccharide maltose that α-(1,4) and α-(1,6) glycosidic link constitute.Regular chemical structure is given the character of pulullan uniqueness, and its structure is rich in toughness and soluble in water.Pulullan can be used as thickening material, and a spot of pulullan is dissolved in the water, just can produce very big viscosity.The transparent film that pulullan is made is grease proofing and can not see through oxygen, and good performance makes it can be used as dressing and the degradable package material of food and medicine.Pulullan can also substitute starch as a kind of low calorie foods, also can use as cosmetic emulsions.Pulullan is a kind of Microbial exopolysaccharides that has application prospect.
Wood sugar has caused investigators' very big concern as a kind of huge potential resources.Existing report utilizes products such as xylose production ethanol, Xylitol, butanols.Yet microorganism utilizes wood sugar efficient generally not high, and the productive rate of these products is also lower.The research Aureobasidium pullulans utilizes the xylose production pulullan that a kind of new path can be provided for the reasonable utilization of wood sugar.At present, Aureobasidium pullulans is produced pulullan and is mainly utilized sucrose and glucose, does not see the report that utilizes the xylose production pulullan.Wood sugar exists with various forms at occurring in nature, for example just contains comparatively considerable wood sugar in xylose mother liquid and the lignocellulose.
The hydrolyzed solution of acid treatment bagasse or corn cob, through two to the concentrating of three-wheel, cooling, the liquid of isolating behind the xylose crystalline body is called xylose mother liquid.35～40% composition is wood sugar in the xylose mother liquid, in addition, also has a spot of glucose, semi-lactosi, pectinose.The annual generation of China surpasses 50,000 tons of xylose mother liquids, and how studying, the liquid that is rich in the wood sugar composition of these cheapnesss of better utilised has profound significance.Be used after adopting physical method that the each component in the xylose mother liquid is separated, complex procedures and cost are higher.Comparatively speaking, the method for bioseparation or bio-transformation has more advantage.
Lignocellulose exists extensively at occurring in nature, and it mainly comprises Mierocrystalline cellulose, hemicellulose, xylogen three class materials.It is the renewable resources of storage capacity maximum on the earth, but the reasonable utilization of lignocellulose is a difficult problem always.Now, have only a spot of lignocellulosic material to be used to industries such as papermaking and chemical industry, great majority are all directly burned or are abandoned.Wood sugar is the glucide that content is only second to glucose in the whole lignocellulose as the primary product of hemicellulose hydrolysate.
These resources that extensively exist can be passed through action of microorganisms, are converted into the material that has more value.Pulullan is used very wide in every profession and trade, have very high commercial value.Thereby the research Aureobasidium pullulans utilizes wood sugar product pulullan just to seem necessary, and it can provide new approaches for the reasonable utilization of wood sugar, can effectively promote its added value.
Summary of the invention:
The method that the invention provides a strain Aureobasidium pullulans bacterial strain and produce pulullan, this bacterial strain can utilize the effective high yield pulullan of wood sugar or xylose mother liquid or hemicellulose hydrolysate.
Technical scheme of the present invention is as follows:
Aureobasidium pullulans AY82 can be that carbon source through fermentation is produced pulullan with the wood sugar, and shake flask fermentation output reaches 16.61g/L, and 5L ferment tank output reaches 16.23g/L.
Aureobasidium pullulans AY82 utilizes the method for xylose production pulullan, comprises the steps:
The preparation of seed liquor: slant strains is cultivated through switching and is obtained seed liquor;
Fermentation culture: seed liquor is inserted fermentation culture in the fermention medium; Fermentation culture is carried out in shaking bottle or fermentor tank; Described fermention medium is main carbon source with wood sugar, adds other nitrogenous sources and trace element composition.
Described fermention medium consists of: wood sugar 40～80g/L, (NH
4)
2SO
40.8～1.2g/L, K
2HPO
44～6g/L, MgSO
47H
2O0.2～0.4g/L, NaCl2～4g/L, yeast soak powder 0.6～1.0g/L, 121 ℃ of high pressure steam sterilization 20min.
Described shake flask fermentation can also be used the xylose mother liquid substratum, consists of: with the wood sugar in the xylose mother liquid replacement fermention medium, described xylose mother liquid composition is as follows: wood sugar 35-40%, pectinose 8-16%, semi-lactosi 9-15%, glucose 8-11%, all the other are water; The total sugar content that is diluted in the xylose mother liquid substratum is 50g/L.
Described shake flask fermentation can also be used the hemicellulose hydrolysate substratum, consists of: with the wood sugar in the hemicellulose hydrolysate replacement fermention medium, described hemicellulose hydrolysate composition is as follows: wood sugar 70%, and glucose 12%, pectinose 7%, all the other are water; The total sugar content that is concentrated in the hemicellulose hydrolysate substratum is 50g/L.
Conditions of flask fermentation is: fermention medium loading amount 20%, and inoculum size 6～10%, shaking speed 180～240r/min, 26～30 ℃ of temperature, initial pH6.0～6.5, fermentation time is 144～168h.
The ferment tank condition is: inoculum size 6～8%, and fermention medium loading amount 60～70%, 26～28 ℃ of culture temperature, rotating speed 200～300r/min, air flow 1.0～1.5vvm, pH controls 5.5 ± 0.1, and tank pressure 0.03Mpa cultivates 7d and finishes fermentation.
The Aureobasidium pullulans AY82(Aureobasidium Pullulan AY82 that utilizes the xylose production pulullan of the present invention), be preserved in China Committee for Culture Collection of Microorganisms common micro-organisms center (being called for short CGMCC) on January 18th, 2013, deposit number is CGMCC No.7154, preservation address: No. 3, No. 1 institute in North Star West Road, Chaoyang District, BeiJing, China city, postcode: 100101.
Aureobasidium pullulans AY82 of the present invention can be applicable to the fermentative production pulullan.
Beneficial effect:
The first, by ultraviolet mutagenesis, get the Aureobasidium pullulans that a strain utilizes wood sugar high yield pulullan at high density wood sugar substratum top sieve, it is less that this bacterial strain produces pigment, is beneficial to the separation and purification of polysaccharide.The second, through Fourier's Infrared spectroscopy, confirm that the polysaccharide of separation and purification is pulullan.The 3rd, utilize bacterial classification of the present invention, the wood sugar shake-flask culture after seven days pulullan output reach 16.61g/L, wood-sugar fermentation substratum 5L fermentor cultivation after seven days output reach 16.23g/L, xylose mother liquid shake flask fermentation pulullan output is 6.73g/L, and hemicellulose hydrolysate shake flask fermentation pulullan output is 9.25g/L.The research Aureobasidium pullulans utilizes wood sugar to produce pulullan, can provide new approaches for the reasonable utilization of wood sugar, can effectively promote its added value.
Description of drawings:
Fig. 1 is the infrared spectrogram of pulullan.
Wherein, A is pulullan mark product, the pulullan that B utilizes wood sugar to produce for Aureobasidium pullulans AY82.
Embodiment:
Below by specific embodiment narration the present invention.Unless stated otherwise, used technique means is method known in those skilled in the art among the present invention.In addition, embodiment is interpreted as illustrative, but not limits the scope of the invention, and the spirit and scope of the invention are only limited by claims.To those skilled in the art, under the prerequisite that does not deviate from essence of the present invention and scope, various changes that the material component in these embodiments and consumption are carried out or change and also belong to protection scope of the present invention.
Embodiment 1: utilize the Aureobasidium pullulans mutant strain screening of wood sugar high yield pulullan
Be starting strain with Aureobasidium pullulans (Aureobasidium Pullulan) CGMCC 3.00837, selecting lethality rate is that 82.4% ultraviolet mutagenesis dosage (irradiation 5min) carries out mutagenesis to Aureobasidium pullulans, to evenly coat on the high density wood sugar flat board (xylose concentration 100g/L) picking mutant strain behind 30 ℃ of lucifuges cultivation 4-5d after the dilution of the bacterium liquid after the mutagenesis.Mutant strain is carried out the multiple sieve of shake flask fermentation experiment, measure the pulullan growing amount behind the fermentation 168h, do contrast with the strain of setting out, obtain the mutant strain AY82 that a strain utilizes wood sugar high yield pulullan, pulullan output has significantly lifting than starting strain, and fermentation the results are shown in Table 1.
Table 1 mutant strain AY82 and starting strain pulullan growing amount are relatively
Embodiment 2: the fermentation of wood sugar culture media shaking vase
Being experimental strain with mutant strain AY82, is carbon source with the wood sugar, and by substratum and fermentation condition optimization, the wood-sugar fermentation substratum that is optimized is: wood sugar 50g/L, (NH
4)
2SO
41.2g/L, K
2HPO
46g/L, MgSO
47H
2O0.4g/L, NaCl2g/L, yeast soak powder 1.0g/L, 121 ℃ of high pressure steam sterilization 20min; The fermentation condition of optimizing is: inoculum size 8%, and shaking speed 200r/min, 28 ℃ of temperature, initial pH value 6.5, incubation time is 168h.
Picking one ring AY82 thalline is seeded to the 250mL triangular flask that the 50mL seed culture medium is housed and cultivates from activated inclined plane, and 28 ℃, 200r/min shaking table cultivation 24～48h get seed liquor.Seed culture medium consists of: wood sugar 20g/L, (NH
4)
2SO
41.0g/L, K
2HPO
44g/L, MgSO
47H
2O0.2g/L, NaCl4g/L, yeast soak powder 2.0g/L, 121 ℃ of high pressure steam sterilization 20min.Seed liquor inserts in the above-mentioned wood-sugar fermentation substratum with 8% inoculum size, and 28 ℃, 200r/min shake flask fermentation are cultivated 168h, and fermentation the results are shown in Table 2.
Described activated inclined plane substratum is: wood sugar 20g/L, yeast soak powder 10g/L, peptone 20g/L, agar 20g/L, pH5.0,121 ℃ of high pressure steam sterilization 20min.
Table 2AY82 wood sugar substratum fermentation result
Embodiment 3: the fermentation of xylose mother liquid culture media shaking vase
The preparation of seed liquor and conditions of flask fermentation are substantially with embodiment 2.The fermention medium component is: xylose mother liquid (wood sugar 35-40%, pectinose 8-16%, semi-lactosi 9-15%, glucose 8-11%) is diluted to that total sugar content is 50g/L, (NH in the substratum
4)
2SO
41.2g/L, K
2HPO
46g/L, MgSO
47H
2O0.4g/L, NaCl2g/L, yeast soak powder 1.0g/L, initial pH6.5.121 ℃ of high pressure steam sterilization 20min.Fermentation the results are shown in Table 3.
Table 3AY82 xylose mother liquid substratum fermentation result
Embodiment 4: the hemicellulose hydrolysate shake flask fermentation
The preparation of seed liquor and conditions of flask fermentation are substantially with embodiment 2.
The fermention medium component is: hemicellulose hydrolysate (wood sugar 70%, glucose 12%, pectinose 7%) is concentrated into that total sugar content is 50g/L, (NH in the substratum
4)
2SO
41.2g/L, K
2HPO
46g/L, MgSO
47H
2O0.4g/L, NaCl2g/L, yeast soak powder 1.0g/L, initial pH6.5.121 ℃ of high pressure steam sterilization 20min.
The preparation method of described hemicellulose hydrolysate is: bagasse powder is broken to about particle diameter 30 orders, presses solid-to-liquid ratio 1:12(quality: the NaOH of adding 0.7% volume), handle 2h under 40 ℃ of conditions.Remove by filter waste liquid, solid substance is cleaned to the oven dry of neutral back.Powder after the oven dry adds the zytase of citric acid solution, 0.01% tween 80 and 20%～30% and at 50 ℃ of following enzymolysis 60h, namely gets hemicellulose hydrolysate by same solid-to-liquid ratio.Wherein, the citrate buffer solution compound method is: get 11.468g Citric acid monohydrate Food grade and 32.54g Na
2HPO
4Be dissolved in the distilled water, and be settled to 1L, transfer pH to 4.8 ± 0.05 standby.
The results are shown in Table 4.
Table 4AY82 hemicellulose hydrolysate substratum fermentation result
The wood-sugar fermentation of embodiment 5:5L fermentor tank
Seed culture medium consists of: wood sugar 20g/L, (NH
4)
2SO
41.0g/L, K
2HPO
44g/L, MgSO
47H
2O0.2g/L, NaCl4g/L, yeast soak powder 2.0g/L, initial pH6.0.121 ℃ of high pressure steam sterilization 20min.
Fermention medium consists of: wood sugar 50g/L, (NH
4)
2SO
41.2g/L, K
2HPO
46g/L, MgSO
47H
2O0.4g/L, NaCl2g/L, yeast soak powder 1.0g/L.121 ℃ of high pressure steam sterilization 20min.
Seed liquor is cultivated: picking one ring AY82 thalline is seeded to the 250mL triangular flask that the 50mL seed culture medium is housed and cultivates from activated inclined plane, and 28 ℃, 200r/min shaking table cultivation 24～48h are as first order seed.First order seed is equipped with in the 500ml triangular flask of 100ml seed culture medium by the access of 8% inoculum size, and 28 ℃, 200r/min shaking table cultivation 24～48h are as secondary seed.
5L ferment tank: secondary seed is linked in the 5L fermentor tank that the 3L fermention medium is housed 28 ℃ of culture temperature, rotating speed 300r/min by 8% inoculum size, air flow 1.5vvm, pH controls 5.5 ± 0.1, tank pressure 0.03Mpa, cultivate 7d and finish fermentation, pulullan output is 16.23g/L.
Fig. 1. product I R collection of illustrative plates (A is pulullan mark product, and B produces pulullan for utilizing wood sugar)
Claims (6)
1. Aureobasidium pullulans AY82 utilizes the method for xylose production pulullan, it is characterized in that, comprises the steps:
The preparation of seed liquor: slant strains is cultivated through switching and is obtained seed liquor;
Fermentation culture: seed liquor is inserted fermentation culture in the fermention medium; Fermentation culture is carried out in shaking bottle or fermentor tank; Described fermention medium is main carbon source with wood sugar, adds other nitrogenous sources and trace element composition; Conditions of flask fermentation is: fermention medium loading amount 20%, and inoculum size 6～10%, shaking speed 180～240r/min, 26～30 ℃ of temperature, initial pH6.0～6.5, fermentation time is 144～168h; The ferment tank condition is: inoculum size 6～8%, and fermention medium loading amount 60～70%, 26～28 ℃ of culture temperature, rotating speed 200～300r/min, air flow 1.0～1.5vvm, pH controls 5.5 ± 0.1, and tank pressure 0.03Mpa cultivates 7d and finishes fermentation.
2. Aureobasidium pullulans AY82 as claimed in claim 1 utilizes the method for xylose production pulullan, it is characterized in that, described fermention medium consists of: wood sugar 40～80g/L, (NH
4)
2SO
40.8～1.2g/L, K
2HPO
44～6g/L, MgSO
47H
2O0.2～0.4g/L, NaCl2～4g/L, yeast soak powder 0.6～1.0g/L, 121 ℃ of high pressure steam sterilization 20min.
3. Aureobasidium pullulans AY82 as claimed in claim 1 or 2 utilizes the method for xylose production pulullan, it is characterized in that, described fermention medium consists of: wood sugar 50g/L, (NH
4)
2SO
41.2g/L, K
2HPO
46g/L, MgSO
47H
2O0.4g/L, NaCl2g/L, yeast soak powder 1.0g/L, 121 ℃ of high pressure steam sterilization 20min.
4. Aureobasidium pullulans AY82 as claimed in claim 1 or 2 utilizes the method for xylose production pulullan, it is characterized in that, described shake flask fermentation xylose mother liquid substratum, consist of: with the wood sugar in the xylose mother liquid replacement fermention medium, described xylose mother liquid composition is as follows: wood sugar 35-40%, pectinose 8-16%, semi-lactosi 9-15%, glucose 8-11%, all the other are water; The total sugar content that is diluted in the xylose mother liquid substratum is 50g/L.
5. Aureobasidium pullulans AY82 as claimed in claim 1 or 2 utilizes the method for xylose production pulullan, it is characterized in that, described shake flask fermentation hemicellulose hydrolysate substratum, consist of: with the wood sugar in the hemicellulose hydrolysate replacement fermention medium, described hemicellulose hydrolysate composition is as follows: wood sugar 70%, glucose 12%, pectinose 7%, all the other are water; The total sugar content that is concentrated in the hemicellulose hydrolysate substratum is 50g/L.
6. Aureobasidium pullulans AY82 as claimed in claim 1 utilizes the method for xylose production pulullan, it is characterized in that, the used seed culture medium of the preparation of described seed liquor consists of: wood sugar 20g/L, (NH
4)
2SO
41.0g/L, K
2HPO
44g/L, MgSO
47H
2O0.2g/L, NaCl4g/L, yeast soak powder 2.0g/L, 121 ℃ of high pressure steam sterilization 20min.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310169915.0A CN103305569B (en) | 2013-05-10 | 2013-05-10 | Method for producing pullulan by aureobasidium pullulans AY82 by using xylose |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310169915.0A CN103305569B (en) | 2013-05-10 | 2013-05-10 | Method for producing pullulan by aureobasidium pullulans AY82 by using xylose |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN103305569A true CN103305569A (en) | 2013-09-18 |
| CN103305569B CN103305569B (en) | 2014-09-10 |
Family
ID=49131289
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201310169915.0A Active CN103305569B (en) | 2013-05-10 | 2013-05-10 | Method for producing pullulan by aureobasidium pullulans AY82 by using xylose |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN103305569B (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112522121A (en) * | 2020-12-23 | 2021-03-19 | 苏州聚维元创生物科技有限公司 | Kluyveromyces and application thereof in producing xylitol |
| US11319566B2 (en) | 2017-04-14 | 2022-05-03 | Capsugel Belgium Nv | Process for making pullulan |
| US11576870B2 (en) | 2017-04-14 | 2023-02-14 | Capsugel Belgium Nv | Pullulan capsules |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4090016A (en) * | 1975-06-17 | 1978-05-16 | Sumitomo Chemical Company, Limited | Carboxylated pullulan and method for producing same |
| CN103060204A (en) * | 2012-12-31 | 2013-04-24 | 天津北洋百川生物技术有限公司 | Mutant strain for mass production of pulullan and cultural method thereof |
-
2013
- 2013-05-10 CN CN201310169915.0A patent/CN103305569B/en active Active
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4090016A (en) * | 1975-06-17 | 1978-05-16 | Sumitomo Chemical Company, Limited | Carboxylated pullulan and method for producing same |
| CN103060204A (en) * | 2012-12-31 | 2013-04-24 | 天津北洋百川生物技术有限公司 | Mutant strain for mass production of pulullan and cultural method thereof |
Non-Patent Citations (3)
| Title |
|---|
| 朱永强: "短梗霉多糖菌的诱变筛选和发酵工艺", 《中国优秀硕士学位论文全文数据库•工程科技I辑》 * |
| 朱永强: "短梗霉多糖菌的诱变筛选和发酵工艺", 《中国优秀硕士学位论文全文数据库•工程科技I辑》, no. 3, 15 March 2012 (2012-03-15) * |
| 相茂功等: "普鲁兰糖在食品工业中的应用进展", 《食品与药品》 * |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US11319566B2 (en) | 2017-04-14 | 2022-05-03 | Capsugel Belgium Nv | Process for making pullulan |
| US11576870B2 (en) | 2017-04-14 | 2023-02-14 | Capsugel Belgium Nv | Pullulan capsules |
| US11878079B2 (en) | 2017-04-14 | 2024-01-23 | Capsugel Belgium Nv | Pullulan capsules |
| CN112522121A (en) * | 2020-12-23 | 2021-03-19 | 苏州聚维元创生物科技有限公司 | Kluyveromyces and application thereof in producing xylitol |
Also Published As
| Publication number | Publication date |
|---|---|
| CN103305569B (en) | 2014-09-10 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Singhania et al. | Developments in bioprocess for bacterial cellulose production | |
| JP4734425B2 (en) | Systems and methods for producing biofuels and related materials | |
| Hargreaves et al. | Production of ethanol 3G from Kappaphycus alvarezii: evaluation of different process strategies | |
| US9309577B2 (en) | Process for producing bio-based product from straw hemicellulose and fully utilizing the components thereof | |
| CN101974436B (en) | Lignocellulose degrading bacteria and application thereof | |
| CN103993042B (en) | A kind of lignocellulose material co-producing bio ethanol and the method for pulullan | |
| CN102174433B (en) | Clostridium beijerinckii with high stress resistance and application thereof | |
| CN103773824B (en) | A kind of fermentation medium for bacterial cellulose and utilize this substratum to produce the method for bacteria cellulose | |
| CN103255067B (en) | Aureobasidium Pullulan producing pullulan with high yield by utilizing xylose and application of Aureobasidium Pullulan | |
| US20240011060A1 (en) | Method For Producing Citric Acid by Degrading Roughages with the Rumen Fungus-methanogen Co-culture from Qinghai Yaks | |
| CN106281862A (en) | The processing method of a kind of Coffee pulp and prepared Coffee pulp fermented wine | |
| CN103305569B (en) | Method for producing pullulan by aureobasidium pullulans AY82 by using xylose | |
| CN108753642A (en) | One plant of production algin catenase bacterial strain Yue Shi Flavobacterium | |
| CN105713851B (en) | One plant of Clostridium beijerinckii and its application | |
| CN103614448B (en) | Method for preparing bioethanol by taking sodium alginate or algae as active ingredients | |
| CN104762229A (en) | A bacillus subtilis strain and applications thereof | |
| CN107446834A (en) | A kind of enterobacteria new strains, acquisition methods and application | |
| Agrawal et al. | Production of an extracellular cellobiase in solid state fermentation | |
| CN102703523B (en) | Method for producing butanol by mixed fermentation of bagasse and molasses serving as raw materials | |
| JP2014176351A (en) | Method for producing ethanol | |
| Zhang et al. | Preparation of γ-polyglutamic acid from enzymatic hydrolysate of poplar sawdust | |
| CN107164246A (en) | A kind of thermotolerant yeast bacterium and its application | |
| CN108330087B (en) | Solid leaven for fermenting peanut straw | |
| CN102492634A (en) | High-temperature resistant yeast and application thereof | |
| CN102433321B (en) | Fused lactobacillus capable of utilizing xylose for fermenting lactic acid and resisting high temperature and breeding method of fused lactobacillus |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant |