CN103301505A - Method for preparing bacterial cellulose three-dimensional exhibition microporous bracket - Google Patents
Method for preparing bacterial cellulose three-dimensional exhibition microporous bracket Download PDFInfo
- Publication number
- CN103301505A CN103301505A CN2013102242886A CN201310224288A CN103301505A CN 103301505 A CN103301505 A CN 103301505A CN 2013102242886 A CN2013102242886 A CN 2013102242886A CN 201310224288 A CN201310224288 A CN 201310224288A CN 103301505 A CN103301505 A CN 103301505A
- Authority
- CN
- China
- Prior art keywords
- bacterial cellulose
- support
- micropore
- dimensional display
- dimensional
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Landscapes
- Materials For Medical Uses (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention discloses a method for preparing a bacterial cellulose three-dimensional exhibition microporous bracket, and relates to the technical field of preparation and processing of bracket materials. The bacterial cellulose bracket is obtained by passivating, cutting and freeze-drying bacterial cellulose produced by fermentation of strains. By a computer tomography technology, a math model of a bacterial cellulose bracket three-dimensional structure is constructed; and the specific three-dimensional exhibition microporous bracket is designed by the math model. The math model for a required bacterial cellulose three-dimensional exhibition microporous structure is introduced into a carbon dioxide laser perforation machine and is processed under an environment of -5-10 DEG C; the processed bacterial cellulose bracket is cleaned by secondary distilled water; and then the bacterial cellulose three-dimensional exhibition microporous bracket is obtained by freeze-drying. According to the method, the technology is simple and convenient to operate; sizes of micropores and structures of three-dimensional exhibition holes of the bracket can be adjusted and controlled by methods, such as a method for controlling technical parameters; and the prepared three-dimensional exhibition microporous bracket can be applied to engineering field of construction of tissues, such as skins, bones, ribs and blood vessels.
Description
Technical field
The present invention relates to the processing technology of preparing field of biologic bracket material.Be particularly related to the preparation method of the three-dimensional display of a kind of Bacterial cellulose micropore support.
Background technology
Tissue engineering bracket material is one of organizational project three big key elements, because tissue all has specific macro morphology, the timbering material that needs to be used for the organizational project reparation also possesses corresponding macro morphology.And can influence growth and the protein expression of cell owing to studies show that timbering material microcosmic (nanoscale, the micron order) structure of repairing for organizational project in a large number.Therefore, in the tissue engineering bracket material preparation process, in the control macro morphology, also need control microstructure.Desirable tissue engineering bracket material should have the 3-D solid structure of the favorable tissue compatibility, biodegradability, degraded avirulence, excellent mechanical intensity and high porosity.Wherein the preparation method of timbering material is often depended in the high porosity of tissue engineering bracket material, high-specific surface area and suitable aperture.At present the preparation method of tissue engineering bracket material mainly contains: method of electrostatic spinning, phase separation method, gas foaming are sent out, solution casting method, percolation etc.Though the tissue engineering bracket that these methods can be succeedd, but resulting tissue engineering bracket lacks, and the mutual perforation degree of mechanical strength, hole is low, the poor controllability of porosity and pore size distribution, varying aperture at random, thereby influence the vascularization of growing into, organizing of cell, the transmission of nutrient and the discharging of metabolite.
Laser boring is the laser processing technology that reaches practicability the earliest, has been widely used in the processing of metal material, inorganic material and macromolecular material.Laser drilling has: untouchable; Punching speed is fast, efficient is high; The aperture is small, adjustable; Be fit to characteristics such as many, the highdensity displays of quantity hole processing.It is tens of to hundreds of microns that the laser micropore aperture can reach, and the organizational project three-dimensional micropore support that adopts this technology to prepare can be conducive to cell and enter internal stent, and nutritional labelings such as convenient egg white matter are passed through, and are convenient to microvascular reconstruction.
The present invention adopts carbon dioxide laser punching technology, prepares the three-dimensional display of a kind of Bacterial cellulose micropore support.Prepared the Bacterial cellulose micropore support of specific three dimensional arrayed at the Bacterial cellulose support with macro morphology.Cell adhesion rate when this timbering material has increased cell culture, and improved the multiplication rate of cell.The present invention prepares fast, and is easy to operate, can be by pore size, shape and the three-dimensional structure that displays the hole of method accuracy controlling supports such as control technological parameter.The three-dimensional display of the Bacterial cellulose of preparation micropore support has the mutual perforation degree height of good mechanical intensity, good structural stability, high porosity and hole, can be used as tissue engineering bracket material, be applied to tissue repair and reconstructions such as skin, bone, cartilage, blood vessel.
Summary of the invention
The invention discloses the preparation method of the three-dimensional display of a kind of Bacterial cellulose micropore support.The processing technology of preparing field that relates to a kind of timbering material.Technology of the present invention is simple, and is easy to operate, can be by the pore size of method regulation and control supports such as control technological parameter and the structure in three-dimensional display hole, and the three-dimensional display micropore support of preparation can be applicable to make up field of tissue engineering technology such as skin, bone, cartilage, blood vessel.
The invention discloses the preparation method of the three-dimensional display of a kind of Bacterial cellulose micropore support.Comprise,
(1) the purified processing of Bacterial cellulose, cutting, the lyophilization that is produced by strain fermentation obtains the Bacterial cellulose support.
(2) adopt computed tomography to make up the mathematical model of Bacterial cellulose support three dimensional structure.Be initial point with the mathematical model center of gravity, the inoculating surfaces during Bacterial cellulose support inoculating cell is projected as the XY plane downwards and sets up three-dimensional system of coordinate.Utilize the specific three-dimensional display microcellular structure of mathematical model design.
(3) the three-dimensional display of required Bacterial cellulose microcellular structure mathematical model is imported in the carbon dioxide laser puncher, under-5～10 ℃ of environment, process, Bacterial cellulose support after the processing cleans through redistilled water, and lyophilization obtains the three-dimensional display of Bacterial cellulose micropore support.
As optimized technical scheme:
Wherein, the preparation method of the three-dimensional display of aforesaid a kind of Bacterial cellulose micropore support, the described bacterial strain that can ferment the generation Bacterial cellulose is one or more in acetobacter xylinum, rhizobium, Sarcina, Rhodopseudomonas, achromobacter, Alcaligenes, Aerobacter or the azotobacter.Described purification treating method can be, tunning heats 3～6h under 30～100 ℃ temperature in percentage by weight is 1～8% NaOH aqueous solution.Water washes repeatedly to neutrality again.To remove tropina and the residual media that sticks on the cellulose membrane.
The preparation method of the three-dimensional display of aforesaid a kind of Bacterial cellulose micropore support, described lyophilization temperature is: under-40～-10 ℃ of conditions.Bacterial cellulose is to be made of the cellulose nano-fibrous of three-dimensional manometer network structure, has only the freeze-drying of employing could keep its original three-dimensional net structure in dry run.In like manner, the Bacterial cellulose support after the processing also needs to adopt the cubical array microcellular structure of freeze-drying to keep obtaining after the processing.
The preparation method of the three-dimensional display of aforesaid a kind of Bacterial cellulose micropore support, described purified processing, cutting, lyophilization obtain its profile of Bacterial cellulose support and are: cylinder, square, cuboid, membranaceous or irregular body.Bacterial cellulose support with certain profile can form in incubation, also can be to be processed to form through machine cuts.
The preparation method of the three-dimensional display of aforesaid a kind of Bacterial cellulose micropore support, the three-dimensional display microcellular structure of described design has three processing planes, respectively XY plane, XY plane, the YZ plane of the mathematical model of corresponding Bacterial cellulose support three dimensional structure.Can realize the array perforation processing of different processing planes by bacteria cellulose material on the change laser-beam drilling machine in the position of anchor clamps.
The preparation method of the three-dimensional display of aforesaid a kind of Bacterial cellulose micropore support, described three-dimensional display microcellular structure: the micropore that is n * n array on the processing plane of every 5mm * 5mm, micro-pore diameter is 100～300 μ m, the micropore spacing is not less than 200 μ m, and the micropore on the inoculating surfaces and XY plane are 45～80 ° angle.In the cell and timbering material recombination process of organizational project, cell inoculation is to drip on timbering material and three-dimensional micropore by material enters timbering material inside by cell suspension.In this course, cell enters the quantity of timbering material and time that cell contacts with timbering material all influences the adhesion rate of cell on timbering material.In order to increase the quantity that cell enters timbering material, when designing, need improve the timbering material microcellular structure interpore mutual perforation degree, can the duct of three processing planes be intersected in duct crossing or three processing planes in twos simultaneously by regulating quantity and the position in three-dimensional display hole.Simultaneously, in order to prolong the time that cell contacts with timbering material, the three-dimensional display microcellular structure of this patent makes micropore and XY plane on the inoculating surfaces be certain included angle.This display micropore design with certain angle of inclination can prevent that the cell suspension passing hole channel from directly spilling timbering material, does not also influence cell suspension and enters timbering material inside.
The preparation method of the three-dimensional display of aforesaid a kind of Bacterial cellulose micropore support, described three-dimensional display micropore perpendicular to cross section radially is: circle, square, triangle or polygon.
Compared with prior art, the invention has the beneficial effects as follows: adopt carbon dioxide laser punching technology, prepare the three-dimensional display of a kind of Bacterial cellulose micropore support.Preparation process can be controlled macro morphology and the microstructure of Bacterial cellulose timbering material, and the arrangement of the cubical array of this Bacterial cellulose micropore support, is conducive to cell and enters timbering material inside, and improved the cell adhesion rate.Can be by pore size, shape and the three-dimensional structure that displays the hole of method accuracy controlling supports such as control technological parameter.The three-dimensional display of the Bacterial cellulose of preparation micropore support has the mutual perforation degree height of good mechanical intensity, good structural stability, high porosity and hole, can be used as tissue engineering bracket material, be applied to tissue repair and reconstructions such as skin, bone, cartilage, blood vessel.
The specific embodiment
Below in conjunction with specific embodiment, further set forth the present invention.Should be understood that these embodiment only to be used for explanation the present invention and be not used in and limit the scope of the invention.Should be understood that in addition those skilled in the art can make various changes or modifications the present invention after the content of having read the present invention's instruction, these equivalent form of values fall within the application's appended claims institute restricted portion equally.
Embodiment 1:
1, will be immersed in percentage by weight by the Bacterial cellulose that the acetobacter xylinum fermentation culture obtains is in 8% the NaOH aqueous solution, to heat 6h under 30 ℃ temperature, and the reuse redistilled water washes repeatedly to neutrality.Remove tropina and the residual media that sticks on the cellulose membrane.Machine cuts is processed into the square of 2cm * 2cm * 2cm.Under-40 ℃ of conditions, lyophilization obtains the Bacterial cellulose support.
2, adopt computed tomography to obtain the faultage image of Bacterial cellulose support, each faultage image is handled, the machine simulation makes up the mathematical model of Bacterial cellulose support three dimensional structure as calculated.Be initial point with the mathematical model center of gravity, the inoculating surfaces during Bacterial cellulose support inoculating cell is projected as the XY plane downwards and sets up three-dimensional system of coordinate.Utilize the three-dimensional display of mathematical model design microcellular structure.The three-dimensional display microcellular structure of design has three processing planes, respectively XY plane, XY plane, the YZ plane of corresponding Bacterial cellulose support three dimensional structure mathematical model.The micropore that is 15 * 15 arrays on the processing plane of every 5mm * 5mm, micro-pore diameter are 100 μ m, and the micropore spacing is not less than 200 μ m, and the micropore on the inoculating surfaces and XY plane are 45 ° angle.Three-dimensional display micropore is circular perpendicular to cross section radially.
3, the three-dimensional display of required Bacterial cellulose microcellular structure mathematical model is imported in the carbon dioxide laser puncher, under-5 ℃ of environment, process, Bacterial cellulose support after the processing cleans through redistilled water, and lyophilization obtains the three-dimensional display of Bacterial cellulose micropore support.
Embodiment 2:
To be immersed in percentage by weight by the Bacterial cellulose that the Sarcina fermentation culture obtains and be in 4% the NaOH aqueous solution, and heat 5h under 60 ℃ temperature, the reuse redistilled water washes repeatedly to neutrality.Remove tropina and the residual media that sticks on the cellulose membrane.It is 1cm that machine cuts is processed into diameter, the cylinder of high 2cm.Under-30 ℃ of conditions, lyophilization obtains the Bacterial cellulose support.
Adopt computed tomography to obtain the faultage image of Bacterial cellulose support, each faultage image is handled, the machine simulation makes up the mathematical model of Bacterial cellulose support three dimensional structure as calculated.Be initial point with the mathematical model center of gravity, the inoculating surfaces during Bacterial cellulose support inoculating cell is projected as the XY plane downwards and sets up three-dimensional system of coordinate.Utilize the three-dimensional display of mathematical model design microcellular structure.The three-dimensional display microcellular structure of design has three processing planes, respectively XY plane, XY plane, the YZ plane of corresponding Bacterial cellulose support three dimensional structure mathematical model.The micropore that is 9 * 9 arrays on the processing plane of every 5mm * 5mm, micro-pore diameter are 300 μ m, and the micropore spacing is not less than 200 μ m, and the micropore on the inoculating surfaces and XY plane are 60 ° angle.Three-dimensional display micropore is square perpendicular to cross section radially.
The three-dimensional display of required Bacterial cellulose microcellular structure mathematical model is imported in the carbon dioxide laser puncher, under 0 ℃ of environment, process, Bacterial cellulose support after the processing cleans through redistilled water, and lyophilization obtains the three-dimensional display of Bacterial cellulose micropore support.
Embodiment 3:
To be immersed in percentage by weight by the Bacterial cellulose that Alcaligenes and azotobacter fermentation culture obtain and be in 1～8% the NaOH aqueous solution, and heat 3h under 100 ℃ temperature, the reuse redistilled water washes repeatedly to neutrality.Remove tropina and the residual media that sticks on the cellulose membrane.Machine cuts is processed into the film material of 5cm * 5cm * 1cm.Under--10 ℃ of conditions, lyophilization obtains the Bacterial cellulose support.
Adopt computed tomography to obtain the faultage image of Bacterial cellulose support, each faultage image is handled, the machine simulation makes up the mathematical model of Bacterial cellulose support three dimensional structure as calculated.Be initial point with the mathematical model center of gravity, the inoculating surfaces during Bacterial cellulose support inoculating cell is projected as the XY plane downwards and sets up three-dimensional system of coordinate.Utilize the three-dimensional display of mathematical model design microcellular structure.The three-dimensional display microcellular structure of design has three processing planes, respectively XY plane, XY plane, the YZ plane of corresponding Bacterial cellulose support three dimensional structure mathematical model.The micropore that is 6 * 6 arrays on the processing plane of every 5mm * 5mm, micro-pore diameter are 200 μ m, and the micropore spacing is not less than 200 μ m, and the micropore on the inoculating surfaces and XY plane are 80 ° angle.Three-dimensional display micropore is equilateral triangle perpendicular to cross section radially.
The three-dimensional display of required Bacterial cellulose microcellular structure mathematical model is imported in the carbon dioxide laser puncher, under 10 ℃ of environment, process, Bacterial cellulose support after the processing cleans through redistilled water, and lyophilization obtains the three-dimensional display of Bacterial cellulose micropore support.
Claims (7)
1. the preparation method of the three-dimensional display of Bacterial cellulose micropore support is characterized in that:
(1) the purified processing of Bacterial cellulose, cutting, the lyophilization that is produced by strain fermentation obtains the Bacterial cellulose support;
(2) adopt computed tomography to make up the mathematical model of Bacterial cellulose support three dimensional structure, be initial point with the mathematical model center of gravity, inoculating surfaces during Bacterial cellulose support inoculating cell is projected as the XY plane downwards and sets up three-dimensional system of coordinate, utilizes the specific three-dimensional display microcellular structure of mathematical model design;
(3) the three-dimensional display of required Bacterial cellulose microcellular structure mathematical model is imported in the carbon dioxide laser puncher, under-5～10 ℃ of environment, process, Bacterial cellulose support after the processing cleans through redistilled water, and lyophilization obtains the three-dimensional display of Bacterial cellulose micropore support.
2. as claim 1, the preparation method of the three-dimensional display of a kind of Bacterial cellulose micropore support is characterized in that: the described bacterial strain that can ferment the generation Bacterial cellulose is one or more in acetobacter xylinum, rhizobium, Sarcina, Rhodopseudomonas, achromobacter, Alcaligenes, Aerobacter or the azotobacter.
3. as claim 1, a kind of Bacterial cellulose three-dimensional displays the preparation method of micropore support, and it is characterized in that: described lyophilization temperature is: under-40～-10 ℃ of conditions.
4. as claim 1, the preparation method of the three-dimensional display of a kind of Bacterial cellulose micropore support, it is characterized in that: described purified processing, cutting, lyophilization obtain its profile of Bacterial cellulose support and are: cylinder, square, cuboid, membranaceous or irregular body.
5. as claim 1, the preparation method of the three-dimensional display of a kind of Bacterial cellulose micropore support, it is characterized in that: the three-dimensional display microcellular structure of design has three processing planes, respectively XY plane, XY plane, the YZ plane of the mathematical model of corresponding Bacterial cellulose support three dimensional structure.
6. as claim 1, the preparation method of the three-dimensional display of a kind of Bacterial cellulose micropore support, it is characterized in that: three-dimensional display microcellular structure: the micropore that is n * n array on the processing plane of every 5mm * 5mm, micro-pore diameter is 100～300 μ m, the micropore spacing is not less than 200 μ m, and the micropore on the inoculating surfaces and XY plane are 45～80 ° angle.
7. as claim 1, the preparation method of the three-dimensional display of a kind of Bacterial cellulose micropore support is characterized in that: three-dimensional display micropore perpendicular to cross section radially is: circle, square, triangle or polygon.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310224288.6A CN103301505B (en) | 2013-06-07 | 2013-06-07 | Method for preparing bacterial cellulose three-dimensional exhibition microporous bracket |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310224288.6A CN103301505B (en) | 2013-06-07 | 2013-06-07 | Method for preparing bacterial cellulose three-dimensional exhibition microporous bracket |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN103301505A true CN103301505A (en) | 2013-09-18 |
| CN103301505B CN103301505B (en) | 2014-11-19 |
Family
ID=49127509
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201310224288.6A Active CN103301505B (en) | 2013-06-07 | 2013-06-07 | Method for preparing bacterial cellulose three-dimensional exhibition microporous bracket |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN103301505B (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103691005A (en) * | 2013-12-24 | 2014-04-02 | 华东交通大学 | Micro-nanofiber tissue engineering scaffold and preparation method thereof |
| CN104958782A (en) * | 2015-06-08 | 2015-10-07 | 东华大学 | Bacterial cellulose porous foamed material and preparation method thereof |
| CN111001951A (en) * | 2019-12-26 | 2020-04-14 | 上海百心安生物技术有限公司 | Vascular stent structure beneficial to wall adhesion and processing device and method thereof |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20030219485A1 (en) * | 2002-05-15 | 2003-11-27 | Sun Pharmaceutical Industries Limited | Oral osmotic controlled drug delivery system |
| CN101288778A (en) * | 2008-06-18 | 2008-10-22 | 天津大学 | Preparation method of porous bacteria cellulose sponges |
| CN101455862A (en) * | 2007-12-12 | 2009-06-17 | 中国科学院金属研究所 | Preparation method of polyporous material for biological medicine tissue engineering scaffold |
| CN102078642A (en) * | 2011-01-19 | 2011-06-01 | 北京大学第三医院 | Articular cartilage restoration and regeneration stent and preparation method thereof |
-
2013
- 2013-06-07 CN CN201310224288.6A patent/CN103301505B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20030219485A1 (en) * | 2002-05-15 | 2003-11-27 | Sun Pharmaceutical Industries Limited | Oral osmotic controlled drug delivery system |
| CN101455862A (en) * | 2007-12-12 | 2009-06-17 | 中国科学院金属研究所 | Preparation method of polyporous material for biological medicine tissue engineering scaffold |
| CN101288778A (en) * | 2008-06-18 | 2008-10-22 | 天津大学 | Preparation method of porous bacteria cellulose sponges |
| CN102078642A (en) * | 2011-01-19 | 2011-06-01 | 北京大学第三医院 | Articular cartilage restoration and regeneration stent and preparation method thereof |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103691005A (en) * | 2013-12-24 | 2014-04-02 | 华东交通大学 | Micro-nanofiber tissue engineering scaffold and preparation method thereof |
| CN103691005B (en) * | 2013-12-24 | 2015-10-28 | 华东交通大学 | A kind of micro--Na fibrous tissue engineering rack and preparation method thereof |
| CN104958782A (en) * | 2015-06-08 | 2015-10-07 | 东华大学 | Bacterial cellulose porous foamed material and preparation method thereof |
| CN104958782B (en) * | 2015-06-08 | 2018-01-02 | 东华大学 | A kind of bacterial cellulose porous foam material and preparation method thereof |
| CN111001951A (en) * | 2019-12-26 | 2020-04-14 | 上海百心安生物技术有限公司 | Vascular stent structure beneficial to wall adhesion and processing device and method thereof |
| CN111001951B (en) * | 2019-12-26 | 2022-02-18 | 上海百心安生物技术股份有限公司 | Vascular stent structure beneficial to wall adhesion and processing device and method thereof |
Also Published As
| Publication number | Publication date |
|---|---|
| CN103301505B (en) | 2014-11-19 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| US20200164103A1 (en) | Cellulose nanofibrillar bioink for 3d bioprinting for cell culturing, tissue engineering and regenerative medicine applications | |
| Jana et al. | Chitosan scaffolds with unidirectional microtubular pores for large skeletal myotube generation | |
| CN102512710B (en) | Preparation method of porous three-dimensional silk fibroin material | |
| CN103301505B (en) | Method for preparing bacterial cellulose three-dimensional exhibition microporous bracket | |
| CN101584882B (en) | Vascular stent material of tissue engineering and manufacturing method thereof | |
| CN102430155A (en) | Cellular silk fibroin porous scaffold, and preparation method thereof | |
| CN101264341A (en) | Three-dimensional porous tissue engineering bracket material, preparation and application thereof | |
| CN103044693A (en) | Preparation method for bacterial cellulose/polyvinyl alcohol composite hydrogel | |
| CN101810878A (en) | Nano-fiber tubular scaffold with multi-stage porous structure | |
| CN112961392A (en) | Preparation method of bionic layered material based on microbial mineralization | |
| CN102274549A (en) | Method for preparing bacteria cellulose bracket material and product thereof | |
| CN103272265B (en) | Bacterial cellulose three-dimensional microporous scaffold preparation method | |
| Zhang et al. | Fabrication of biomimetic scaffolds with oriented porous morphology for cardiac tissue engineering | |
| CN106390197B (en) | One kind stress active mass's engineering scaffold material and preparation method thereof | |
| Salihu et al. | Overview of inexpensive production routes of bacterial cellulose and its applications in biomedical engineering | |
| CN102631703A (en) | Three-dimensional non-support bone repairing patch and preparation method thereof | |
| CN108553689B (en) | Silk fibroin porous microsphere with nanofiber microstructure and preparation method thereof | |
| CN103272266B (en) | Preparation method of bacterial cellulose three-dimensional display porous scaffold | |
| CN204364503U (en) | Poroelasticity polyvinyl alcohol/Nano bacteria cellulose compound water congealing sebific duct | |
| CN203736584U (en) | Macroporous three-dimensional ordered-orientation silk fibroin nanofiber support | |
| BR112022025249A2 (en) | BIOLOGICAL SYSTEMS AND METHODS AND ALGAE COLLECTION AND CULTIVATION | |
| Liang et al. | Nanocellulose-based materials for biomedical applications | |
| TWI421339B (en) | Method of fabricating three dimensional scaffolds and device thereof | |
| CN106581775A (en) | Natural fibroin protein fiber scaffold and preparation method | |
| Graup | Production and Characterisation of Tissue-mimicking Collagen Scaffolds for Cardiac Tissue Engineering |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant |