CN103299827A - Method for preparing dietary fiber from ganoderma lingzi residue through ganoderma lingzi solid fermentation - Google Patents
Method for preparing dietary fiber from ganoderma lingzi residue through ganoderma lingzi solid fermentation Download PDFInfo
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- CN103299827A CN103299827A CN2013102290818A CN201310229081A CN103299827A CN 103299827 A CN103299827 A CN 103299827A CN 2013102290818 A CN2013102290818 A CN 2013102290818A CN 201310229081 A CN201310229081 A CN 201310229081A CN 103299827 A CN103299827 A CN 103299827A
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Abstract
The invention provides a method for producing and preparing high-quality ganoderma lingzi type dietary fiber by taking ganoderma lingzi extract residues which are rich in dietary fiber and very low in soluble dietary fiber as the raw material, and by modifying the ganoderma lingzi extract residue dietary fiber through ganoderma lingzi solid fermentation. The production method provided by the invention is low in raw material cost, simple in equipment and operation, capable of realizing large-scale production, beneficial for comprehensive utilization of ganoderma lingzi, and capable of turning waste into wealth. The whole production process is gentle in condition, intake of toxic and harmful substances is prevented, and not only is the physiological activity of the extracted dietary fiber maintained to the maximum extent, but also the extracted dietary fiber is high in security.
Description
(1) technical field
The present invention relates to a kind of method of utilizing glossy ganoderma solid fermentation glossy ganoderma residue to prepare dietary fiber.
(2) background technology
Glossy ganoderma is the famous and precious medicinal fungi of China, has the effect such as strengthen the body resistance to consolidate the constitution, and is called top grade by " herbal classic ".Modern medicine study finds, the effects such as GL-B has the immunity of raising, eliminates free radical, antitumor, radioresistance are one of crucial effective components of glossy ganoderma.In recent years, the ganoderma lucidum product with exact function of exploitation high-tech content becomes focus, the GL-B series products gains great popularity because of its significant effect, the huge market demand, therefore produce a large amount of glossy ganodermas and extract residue, pass into disuse to environment, also to be a kind of very large wasting of resources.
The constituent analysis of glossy ganoderma being extracted residue shows, glossy ganoderma is extracted the residue dietary fiber content up to 84.6%, and take insoluble diedairy fiber as main, is a kind of good dietary fiber sources.Dietary fiber is listed in " the 7th nutrient ".The expert studies show that, still lacks dietary fiber 10g～15g in Chinese's normal diet every day, and the shortage of dietary fiber will cause various diseases.At present, diet fiber product is mainly take the vegetable diet fiber additive as main on the market, and tool is necessarily functional but consumption is relatively poor, lacks to supply mass consumption, the function diet fiber product that can directly eat.
Studies show that, microorganism especially edible and medicinal fungi has the special biological metabolism enzyme system of degraded, the insoluble trophic component of trans-utilization.During the fermentation, mycelia secretion extracellular enzyme can be the bioactive dietary fiber that has more take microbial polysaccharide, cellulose etc. as the master with the conversions such as cellulose, hemicellulose, lignin in the dietary fiber sources by enzymolysis.Glossy ganoderma has powerful cellulose, Ligninolytic Enzymes, and existing glossy ganoderma biofermentation prepares the report of the dietary fibers such as slag, celery residue, maize peel slag, sweet potato dregs, purple sweet potato dregs of fainting, and has obtained preferably dietary fiber modification effect.
Glossy ganoderma is extracted lignin in the slag, content of cellulose is high, is conducive to the grown cultures of glossy ganoderma.Therefore utilize and contain the abundant and glossy ganoderma that soluble dietary fiber is extremely low of dietary fiber and extract slag and be raw material, through the glossy ganoderma solid fermentation glossy ganoderma is extracted dregs diet fibre and carry out modification, produce high-quality glossy ganoderma type dietary fiber, not only can alleviate environmental pollution, economizing on resources, also is the complete utilization effective way that realizes glossy ganoderma.
(3) summary of the invention
Preparing GL-B for present glossy ganoderma deep processing exists extraction efficiency low, the wasting of resources is large, extract slag and easily cause the problems such as environmental pollution, the invention provides a kind of to contain that dietary fiber is abundant and glossy ganoderma that soluble dietary fiber is extremely low is extracted slag as raw material, utilize the glossy ganoderma solid fermentation that glossy ganoderma is extracted dregs diet fibre and carry out modification, the method of the glossy ganoderma type dietary fiber that manufacture is high-quality turns waste into wealth, and realizes the complete utilization of glossy ganoderma.
The technical solution used in the present invention is:
A kind of method of utilizing glossy ganoderma solid fermentation glossy ganoderma residue to prepare dietary fiber, described method comprises:
(1) lucidum strain is seeded to the productive culture base that contains the glossy ganoderma residue, 25～30 ℃ of constant temperature culture 15～30d; Described productive culture matrix amount is composed as follows: 8～45 parts of bagasse powders, and 10～25 parts in wheat bran, 1 part of land plaster, 0.2 part in magnesium sulfate, 30～80 parts of glossy ganoderma residues, moisture 55%～65%(moisture accounts for the ratio of medium gross mass); Described glossy ganoderma residue is that glossy ganoderma (Ganoderma luncidum) is extracted polysaccharide by residue powder dry, after pulverizing; It is as follows that glossy ganoderma is extracted polysaccharide process: REISH, add 10～20 times of quality distilled water mixings, transfer pH6.5～7.5, (600～800W) process 20～30min to microwave, filter, filtrate is concentrated rear alcohol extract polysaccharide further, after filter residue drying, the pulverizing, be the glossy ganoderma residue, form as productive culture base of the present invention;
The productive culture base resistant to elevated temperatures polyethylene plastic bag of packing into, every bag of 0.5～1.0kg fraps sack, inoculates behind 130 ℃ of sterilization 2～4h; Treat that mycelium sends out completely full bag, full bag becomes white or milky, and color and luster is even, fermentation ends;
(2) after the fermentation ends, take out the solid mycoplasma, dry, pulverize, grind, and add distilled water by feed liquid mass ratio 1:5～15,70～90 ℃ of ultrasonic extraction 0.5～2h, filtrate and filter residue are collected respectively in filtration;
(3) step (2) filtrate decompression is concentrated into 1/4～1/2 of original volume, and the gained concentrate is pressed 1:3～8 volume ratios for adding 95% ethanol, leave standstill more than 6 hours, and abandoning supernatant, sediment is concentrated, and atomized drying obtains water-soluble dietary fiber;
(4) step (2) filter residue adds distilled water by feed liquid mass ratio 1:12～18, regulates pH to 8.0～12.0, and suction filtration behind 50～100 ℃ of lower stirring in water bath 60～180min is got filter residue, obtains insoluble diedairy fiber.
Preferably, described productive culture matrix amount is composed as follows: 10～30 parts of bagasse powders, 15～25 parts in wheat bran, 1 part of land plaster, 0.2 part in magnesium sulfate, 40～75 parts of glossy ganoderma residues, moisture 55%～65%.
Described glossy ganoderma need to carry out seed culture usually before fermentation, obtain according to a conventional method to access the productive culture base after the seed liquor again and carry out fermented and cultured.
Among the present invention, glossy ganoderma seed liquor procurement process can be as follows:
(1) actication of culture: lucidum strain is inoculated in the PDA slant medium, at 25～30 ℃ of constant temperature culture 8～15d, puts 4 ℃ of refrigerators for subsequent use.The PDA slant medium is composed as follows: potato 200g/L, and glucose 20g/L, agar 20g/L, solvent are running water, pH nature, 121 ℃ of sterilization 20～30min.
(2) seed culture: with the inclined-plane ganoderma lucidum mycelium access seed culture fluid of (1) activation, shaken cultivation 4～8d under 25～30 ℃, 200r/min gets seed liquor.Seed culture fluid is composed as follows: glucose 30g/L, yeast extract 2g/L, potassium dihydrogen phosphate 1g/L, MgSO
47H
2O 0.5g/L, VB1 0.05g/L, solvent are running water, pH nature, 121 ℃ of sterilization 20～30min.
Beneficial effect of the present invention is mainly reflected in:
The one-tenth of residue was grouped into and the grown cultures characteristics of glossy ganoderma after the present invention extracted according to GL-B, and preparation is extracted slag as main medium take GL-B, and the inoculation lucidum strain carries out the solid fermentation Degradation and Transformation and produces dietary fiber.The special biological metabolism enzyme system of utilizing glossy ganoderma to produce is during the fermentation decomposed the glossy ganoderma residue, residual glossy ganoderma dietary fiber, the polysaccharide of extraction slag discharged, and can produce new mycelium polysaccharides, dietary fiber, obtain the glossy ganoderma type dietary fiber that a kind of function is improved, the glossy ganoderma type dietary fiber of the present invention's preparation not only activity improves, and recovery rate is up to 92%, and the ratio of water insoluble dietary fiber and water-soluble dietary fiber reaches 4:1.
The production method that the present invention adopts, cost of material is low, and equipment and simple to operate can be accomplished scale production, and is conducive to the full use of glossy ganoderma, turns waste into wealth.Whole production process mild condition does not have the absorption of harmful toxic matter, not only can make the dietary fiber that extracts farthest keep its physiologically active, also to extract dietary fiber safe.
(4) description of drawings
Fig. 1 is that glossy ganoderma solid fermentation biological transformation glossy ganoderma is extracted the flow chart that slag prepares dietary fiber;
Fig. 2 is the process chart that dietary fiber extracts.
(5) embodiment
The present invention is described further below in conjunction with specific embodiment, but protection scope of the present invention is not limited in this:
Embodiment 1:
1, productive culture matrix manufacturing:
1. quality prescription: 10 parts of bagasse powders, 15 parts in wheat bran, 1 part of land plaster, 0.2 part in magnesium sulfate, 73.8 parts of glossy ganoderma residues, moisture 65%(moisture account for the ratio of medium gross mass); Described glossy ganoderma residue is that glossy ganoderma (Ganoderma luncidum) (holy sesame No. 1, Institute of Microorganism, Academia Sinica identify bacterial strain) is extracted polysaccharide by residue powder dry, after pulverizing; It is as follows that glossy ganoderma is extracted polysaccharide process: REISH, add 10 times of quality distilled water mixings, and transfer pH6.5, microwave (600W) is processed 30min, filters, and filtrate is concentrated rear alcohol extract polysaccharide further, after filter residue drying, the pulverizing, namely form as productive culture base of the present invention;
2. culture medium prescription mixes in will be 1., adds running water to moisture 65%, the productive culture base resistant to elevated temperatures polyethylene plastic bag of packing into, and every bag of 0.5kg fraps sack, 130 ℃ of sterilization 2h;
2, actication of culture: lucidum strain (holy sesame No. 1) is inoculated in the PDA slant medium, and 25 ℃ of constant temperature culture 15d put 4 ℃ of refrigerators for subsequent use.The PDA slant medium is composed as follows: potato 200g/L, and glucose 20g/L, agar 20g/L, solvent are running water, pH nature, 121 ℃ of sterilization 20min.
3, seed culture: the ganoderma lucidum mycelium of step 2 activation culture is inoculated in the seed culture fluid, and shaken cultivation 8d under 25～30 ℃, 200r/min gets seed liquor.Seed culture fluid is composed as follows: glucose 30g/L, yeast extract 2g/L, potassium dihydrogen phosphate 1g/L, MgSO
47H
2O 0.5g/L, VB1 0.05g/L, solvent are running water, pH nature, 121 ℃ of sterilization 30min.
4, the inoculation of productive culture base and cultivation: get the cultured seed liquor of step 3 and evenly be inoculated in productive culture base (10mL/ bag) with the aseptic inoculation rifle, 25 ℃ of constant temperature culture 30d treat that mycelium sends out completely full bag, and full bag becomes white or milky, and color and luster is even, fermentation ends.
5, the extraction of dietary fiber:
1. take out the solid mycoplasma, 60 ℃ of oven dry, oven dry, pulverizing, grinding, and by feed liquid mass ratio 1:10 adding distilled water, 70 ℃ of ultrasonic extraction 2h filter, and collect respectively filtrate and filter residue;
2. get step 1. filtrate adopt the economic benefits and social benefits concentrator to collect filtrate decompression to be concentrated into 1/4 of original volume, the gained concentrate is pressed the 1:4 volume ratio for adding 95%(v/v) the ethanol water precipitation, left standstill abandoning supernatant 12 hours, sediment is concentrated, and atomized drying obtains water-soluble dietary fiber;
3. get step 1. filter residue add distilled water by feed liquid mass ratio 1:12, regulate pH to 8.5, suction filtration behind 80 ℃ of lower stirring in water bath 180min is got filter residue, obtains insoluble diedairy fiber.
The recovery rate of this example total dietary fiber is 85%, and the ratio of water insoluble dietary fiber water insoluble dietary fiber and water-soluble dietary fiber is 5:1.
Embodiment 2:
1, productive culture matrix manufacturing:
1. quality prescription: 15 parts of bagasse powders, 20 parts in wheat bran, 1 part of land plaster, 0.2 part in magnesium sulfate, 63.8 parts of glossy ganoderma residues, moisture 60%(moisture account for the ratio of medium gross mass); Described glossy ganoderma residue is that glossy ganoderma (Ganoderma luncidum) (ACCC51376 is available from ACCC) is extracted polysaccharide by residue powder dry, after pulverizing; It is as follows that glossy ganoderma is extracted polysaccharide process: REISH, add 15 times of quality distilled water mixings, and transfer pH7.0, microwave (700W) is processed 25min, filters, and filtrate is concentrated rear alcohol extract polysaccharide further, after filter residue drying, the pulverizing, namely form as productive culture base of the present invention;
2. culture medium prescription mixes in will be 1., adds running water to moisture 60%, the productive culture base resistant to elevated temperatures polyethylene plastic bag of packing into, and every bag of 0.8kg fraps sack, 130 ℃ of sterilization 3h;
2, actication of culture: lucidum strain (ACCC51376) is inoculated in the PDA slant medium, and 28 ℃ of constant temperature culture 12d put 4 ℃ of refrigerators for subsequent use.The PDA slant medium is composed as follows: potato 200g/L, and glucose 20g/L, agar 20g/L, solvent are running water, pH nature, 121 ℃ of sterilization 30min.
3, seed culture: the ganoderma lucidum mycelium of step 2 activation culture is inoculated in the seed culture fluid, and shaken cultivation 6d under 28 ℃, 200r/min gets seed liquor.Seed culture fluid is composed as follows: glucose 30g/L, yeast extract 2g/L, potassium dihydrogen phosphate 1g/L, MgSO
47H
2O 0.5g/L, VB1 0.05g/L, solvent are running water, pH nature, 121 ℃ of sterilization 30min.
4, the inoculation of productive culture base and cultivation: get the cultured seed liquor of step 3 and evenly be inoculated in productive culture base (15mL/ bag) with the aseptic inoculation rifle, 28 ℃ of constant temperature culture 20d treat that mycelium sends out completely full bag, and full bag becomes white or milky, and color and luster is even, fermentation ends.
5, the extraction of dietary fiber:
1. take out the solid mycoplasma, 60 ℃ of oven dry, oven dry, pulverizing, grinding, and by feed liquid mass ratio 1:12 adding distilled water, 80 ℃ of ultrasonic extraction 1.5h filter, and collect respectively filtrate and filter residue;
2. get step 1. filtrate adopt the economic benefits and social benefits concentrator to collect filtrate decompression to be concentrated into 1/3 of original volume, the gained concentrate is pressed the 1:6 volume ratio for adding 95% ethanol precipitation, leaves standstill more than 6 hours abandoning supernatant, sediment is concentrated, and atomized drying obtains water-soluble dietary fiber;
3. get step 1. filter residue add distilled water by feed liquid mass ratio 1:15, regulate pH to 9.5, suction filtration behind 90 ℃ of lower stirring in water bath 150min is got filter residue, obtains insoluble diedairy fiber.
The recovery rate of this example total dietary fiber is 92%, and the ratio of water insoluble dietary fiber water insoluble dietary fiber and water-soluble dietary fiber reaches 4:1.
Embodiment 3:
1, productive culture matrix manufacturing:
1. quality prescription: 30 parts of bagasse powders, 25 parts in wheat bran, 1 part of land plaster, 0.2 part in magnesium sulfate, 43.8 parts of glossy ganoderma residues, moisture 55%(moisture account for the ratio of medium gross mass); Described glossy ganoderma residue is that glossy ganoderma (Ganoderma luncidum) (ACCC51722 is available from ACCC) is extracted polysaccharide by residue powder dry, after pulverizing; It is as follows that glossy ganoderma is extracted polysaccharide process: REISH, add 20 times of quality distilled water mixings, and transfer pH7.5, microwave (800W) is processed 20min, filters, and filtrate is concentrated rear alcohol extract polysaccharide further, after filter residue drying, the pulverizing, namely form as productive culture base of the present invention;
2. culture medium prescription mixes in will be 1., adds running water to moisture 55%, the productive culture base resistant to elevated temperatures polyethylene plastic bag of packing into, and every bag of 1.0kg fraps sack, 130 ℃ of sterilization 4h;
2, actication of culture: lucidum strain (ACCC51722) is inoculated in the PDA slant medium, and 30 ℃ of constant temperature culture 8d put 4 ℃ of refrigerators for subsequent use.The PDA slant medium is composed as follows: potato 200g/L, and glucose 20g/L, agar 20g/L, solvent are running water, pH nature, 121 ℃ of sterilization 30min.
3, seed culture: the ganoderma lucidum mycelium of step 2 activation culture is inoculated in the seed culture fluid, and at 30 ℃, shaken cultivation 4d gets seed liquor under the 200r/min.Seed culture fluid is composed as follows: glucose 30g/L, yeast extract 2g/L, potassium dihydrogen phosphate 1g/L, MgSO
47H
2O 0.5g/L, VB1 0.05g/L, solvent are running water, pH nature, 121 ℃ of sterilization 30min.
4, the inoculation of productive culture base and cultivation: get the cultured seed liquor of step 3 and evenly be inoculated in productive culture base (20mL/ bag) with the aseptic inoculation rifle, 30 ℃ of constant temperature culture 15d treat that mycelium sends out completely full bag, and full bag becomes white or milky, and color and luster is even, fermentation ends.
5, the extraction of dietary fiber:
1. take out the solid mycoplasma, 60 ℃ of oven dry, oven dry, pulverizing, grinding, and by feed liquid mass ratio 1:15 adding distilled water, 90 ℃ of ultrasonic extraction 0.5h filter, and collect respectively filtrate and filter residue;
2. get step 1. filtrate adopt the economic benefits and social benefits concentrator to collect filtrate decompression to be concentrated into 1/2 of original volume, the gained concentrate is pressed the 1:8 volume ratio for adding 95% ethanol precipitation, leaves standstill more than 6 hours abandoning supernatant, sediment is concentrated, and atomized drying obtains water-soluble dietary fiber;
3. get step 1. filter residue add distilled water by feed liquid mass ratio 1:18, regulate pH to 11.5, suction filtration behind 100 ℃ of lower stirring in water bath 60min is got filter residue, obtains insoluble diedairy fiber.
The recovery rate of this example total dietary fiber is 90%, and the ratio of water insoluble dietary fiber water insoluble dietary fiber and water-soluble dietary fiber reaches 6:1.
Claims (2)
1. method of utilizing glossy ganoderma solid fermentation glossy ganoderma residue to prepare dietary fiber, described method comprises:
(1) lucidum strain is seeded to the productive culture base that contains the glossy ganoderma residue, 25～30 ℃ of constant temperature culture 15～30d; Described productive culture matrix amount is composed as follows: 8～45 parts of bagasse powders, 10～25 parts in wheat bran, 1 part of land plaster, 0.2 part in magnesium sulfate, 30～80 parts of glossy ganoderma residues, moisture 55%～65%;
(2) after the fermentation ends, take out the solid mycoplasma, dry, pulverize, grind, and add distilled water by feed liquid mass ratio 1:5～15,70～90 ℃ of ultrasonic extraction 0.5～2h, filtrate and filter residue are collected respectively in filtration;
(3) step (2) filtrate decompression is concentrated into 1/4～1/2 of original volume, and the gained concentrate is pressed 1:3～8 volume ratios for adding 95% ethanol, leave standstill more than 6 hours, and abandoning supernatant, sediment is concentrated, and atomized drying obtains water-soluble dietary fiber;
(4) step (2) filter residue adds distilled water by feed liquid mass ratio 1:12～18, regulates pH to 8.0～12.0, and suction filtration behind 50～100 ℃ of lower stirring in water bath 60～180min is got filter residue, obtains insoluble diedairy fiber.
2. the method for claim 1 is characterized in that described productive culture matrix amount is composed as follows: 10～30 parts of bagasse powders, 15～25 parts in wheat bran, 1 part of land plaster, 0.2 part in magnesium sulfate, 40～75 parts of glossy ganoderma residues, moisture 55%～65%.
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| CN104130948A (en) * | 2014-07-11 | 2014-11-05 | 福建农林大学 | Preparation method and application of Antrodia camphorate mycelium medium |
| CN104814407A (en) * | 2015-05-18 | 2015-08-05 | 青岛海之星生物科技有限公司 | Nutrition powder based on sweet potatoes and preparation method of nutrition powder |
| CN104814385A (en) * | 2015-05-18 | 2015-08-05 | 青岛海之星生物科技有限公司 | Healthcare sweet potato powder and preparation method thereof |
| CN106820134A (en) * | 2017-02-21 | 2017-06-13 | 南京晓庄学院 | A kind of preparation method and purposes of the immunopotentiator based on sesame stilbene mycoplasma |
| CN106995337A (en) * | 2017-03-14 | 2017-08-01 | 成都大学 | A kind of Ganoderma lucidum by submerged culture base of high yield GL-B and ganoderic acid and preparation method thereof |
| CN110124619A (en) * | 2019-05-16 | 2019-08-16 | 重庆三峡学院 | A kind of preparation method of heavy metal lead adsorbent |
| CN111449249A (en) * | 2020-05-19 | 2020-07-28 | 山西金科海生物制品有限公司 | Preparation method of sea-buckthorn insoluble dietary fiber |
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104130948A (en) * | 2014-07-11 | 2014-11-05 | 福建农林大学 | Preparation method and application of Antrodia camphorate mycelium medium |
| CN104814407A (en) * | 2015-05-18 | 2015-08-05 | 青岛海之星生物科技有限公司 | Nutrition powder based on sweet potatoes and preparation method of nutrition powder |
| CN104814385A (en) * | 2015-05-18 | 2015-08-05 | 青岛海之星生物科技有限公司 | Healthcare sweet potato powder and preparation method thereof |
| CN106820134A (en) * | 2017-02-21 | 2017-06-13 | 南京晓庄学院 | A kind of preparation method and purposes of the immunopotentiator based on sesame stilbene mycoplasma |
| CN106995337A (en) * | 2017-03-14 | 2017-08-01 | 成都大学 | A kind of Ganoderma lucidum by submerged culture base of high yield GL-B and ganoderic acid and preparation method thereof |
| CN110124619A (en) * | 2019-05-16 | 2019-08-16 | 重庆三峡学院 | A kind of preparation method of heavy metal lead adsorbent |
| CN111449249A (en) * | 2020-05-19 | 2020-07-28 | 山西金科海生物制品有限公司 | Preparation method of sea-buckthorn insoluble dietary fiber |
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Application publication date: 20130918 |