CN103293202A - Method for rapidly detecting forchlorfenuron residue in fruit juice - Google Patents
Method for rapidly detecting forchlorfenuron residue in fruit juice Download PDFInfo
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- CN103293202A CN103293202A CN2013102164603A CN201310216460A CN103293202A CN 103293202 A CN103293202 A CN 103293202A CN 2013102164603 A CN2013102164603 A CN 2013102164603A CN 201310216460 A CN201310216460 A CN 201310216460A CN 103293202 A CN103293202 A CN 103293202A
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- cppu
- electrolytic cell
- fruit juice
- forchlorfenuron
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Abstract
The invention relates to a method for rapidly detecting forchlorfenuron residue in fruit juice. According to the technical scheme of the invention, the method comprises the steps of: adding a phosphate buffered solution into an electrolytic cell, adding a forchlorfenuron solution into the electrolytic cell, starting a magnetic stirrer, setting constant potential and enrichment time of electrode pretreatment, after the enrichment is completed, putting a working electrode, a reference electrode and an auxiliary electrode into the electrolytic cell containing 10ml of phosphate buffered solution, and measuring peak current signals. The method can be used for overcoming the defects that in the prior art, the emulsification phenomenon is easy to generate, the steps of purification treatment are relatively complicated, the purification effect is not ideal, the requirements for instruments and equipment are high and the like. According to the method, the sample treatment is easy and feasible, the instruments are stable in performance, and the results are reliable and can be directly displayed without needing professionals; the consumed time is short, the detection cost is low, the degree of automation is high, the operation is easy and convenient, the sampling amount is small, the method is especially applicable to the detection of forchlorfenuron content in plants, and juice original samples can be directly taken for analysis without a complex pretreatment process.
Description
Technical field
The present invention relates to a kind of based on electrochemical method, the particularly residual method of CPPU in a kind of fast detecting fruit juice.
Background technology
In recent years, CPPU is applied to the production of melon and fruit more and more widely as a kind of exogenous hormone, has brought huge economic benefit for numerous orchard workers and enterprise.But it has also brought stern challenge and threat to agricultural product security simultaneously.
Before the present invention, the existing residual quantity that adopts CPPU in high effective liquid chromatography for measuring watermelon, grape and the Kiwi berry.Employing liquid-liquid extraction-high performance liquid chromatography is arranged.Yet this method organic reagent use amount is big, and is easy to generate emulsion in leaching process, and the purified treatment step is loaded down with trivial details relatively.Also the someone adopts high performance liquid chromatography-mass spectrometry method to detect CPPU is residual, and pre-treatment adopts anionite propyl group ethylenediamine that matrix is carried out purified treatment, but clean-up effect is undesirable, and the method is had relatively high expectations to instrument and equipment.
Summary of the invention
Purpose of the present invention just is to overcome above-mentioned defective, and development is based on the device of electrochemical method determining CPPU.
Technical scheme of the present invention is:
The residual method of CPPU in a kind of fast detecting fruit juice, its major technique step is:
(1) phosphate buffered solution is joined in the electrolytic cell;
(2) CPPU solution is added in this electrolytic cell;
(3) start magnetic stirring apparatus;
(4) the pretreated constant potential of electrode, enrichment time are set;
(5) after enrichment finishes, working electrode, contrast electrode, auxiliary electrode are put into the electrolytic cell that contains the 10ml phosphate buffered solution;
(6) measure the peak current signal.
Described working electrode is carbon electrode, and described contrast electrode is saturated potassium chloride electrode, and described auxiliary electrode is platinum electrode.
Described working electrode is located on the megohmite insulant, and a circle porous mass is located at the outer ring of insulating matter.
Described carbon electrode diameter can be regulated.
Advantage of the present invention and effect are the sample preparation simple possible, get final product with the ethanol dissolving; Instrument performance is stable, and reliable results is easy and simple to handle, the automaticity height, and the result directly shows, does not need the professional; Weak point consuming time, 3min can obtain analysis result, detects that cost is low, automaticity is high, easy and simple to handle and sampling amount is little, is particularly useful for the detection of CPPU content in the plant, can directly take the fruit juice former state analyze, and need not complicated pre-treatment process.
Other concrete advantages of the present invention and effect will go on to say below.
Embodiment
CPPU solution is joined among the PBS, constant potential and enrichment time are set, make CPPU be enriched to carbon electrodes, in blank PBS, measure the current signal of CPPU then.The content of CPPU is proportional to the current signal strength of output, and the instrument of making is self-reacting device, one-shot measurement about 3min consuming time, and reagent dosage can be controlled at 10 μ l.Especially working electrode, contrast electrode and auxiliary electrode are made in the three-electrode system immersion electrolytic cell and detected, thereby both guaranteed the abundant enrichment of the quilt of CPPU in the sample, guarantee few sample size again, when carrying out the CPPU detection, use sample still less like this, thereby also guarantee environment is not polluted.
Electrolytic solution is neutral electrolyte, after CPPU solution is added, can directly measure with apparatus of the present invention.Electrolytic solution is selected neutral phosphate buffered saline(PBS).
Measurement mechanism based on electrochemical method determining CPPU character, comprise that electrochemical workstation, electrolytic cell, working electrode, contrast electrode and auxiliary electrode constitute, it is characterized in that working electrode, contrast electrode and auxiliary electrode are made three-electrode system, working electrode is carbon electrode, can adjust electrode size as required, guarantee to insert littler electrolytic cell or carry out online detection with this, contrast electrode is saturated potassium chloride electrode, and auxiliary electrode is platinum electrode.Contrast electrode places internal-filling liquid, and internal-filling liquid is the saturated potassium chloride solution of routine etc.
During measurement, constant potential and enrichment time are set earlier, make CPPU be enriched to the bare electrode surface, in blank PBS, measure the current signal of CPPU then.Detect lower limit and can be low to moderate 10
-14Mol/L.The size of CPPU concentration is relevant with the current signal strength of output, thereby by arranging of instrument itself result is calculated demonstration (being finished automatically by instrument).
Get 10 μ l CPPU solution and place the electrolytic cell that 10ml PBS (phosphate buffered solution) is housed.
Three electrode new systems are all immersed in the electrolytic solution.Open magnetic stirring apparatus, the pretreated current potential of electrode and enrichment time in the electrochemical workstation are set, make CPPU be enriched to carbon electrodes, treat that enrichment puts into the electrolytic cell that only contains 10ml PBS solution after three-electrode system is clean with distilled water flushing after finishing, pressing measuring switch begins to measure, can obtain the peak current signal of CPPU correspondence behind the 3min, unit is A.Measure and finish.
Electrochemical workstation comprises Survey Software and three electrode interfaces, and interface connects different electrodes respectively, the green working electrode that connects, and white connects contrast electrode, the red auxiliary electrode that connects.Electrolytic solution is selected neutral PBS solution.
In the measurement mechanism of the present invention, the three-electrode system that working electrode, contrast electrode and auxiliary electrode constitute is inserted in the electrolytic cell.Electrolytic cell adopts glass, and working electrode is located on the megohmite insulant; Contrast electrode places internal-filling liquid, and internal-filling liquid is conventional saturated potassium chloride solution, guarantees conduction between electrode internal-filling liquid and the external solution and internal-filling liquid does not ooze out; When the working electrode diameter is big, need more sample and bigger electrolytic cell, but the present invention can adopt the carbon electrode of different area as required, available less reagent can be controlled at 10 μ l as reagent dosage.Described reagent is the each consumption of measuring of CPPU.
Claims (4)
1. the residual method of CPPU in the fast detecting fruit juice, its step is:
(1) phosphate buffered solution is joined in the electrolytic cell;
(2) CPPU solution is added in this electrolytic cell;
(3) start magnetic stirring apparatus;
(4) the pretreated constant potential of electrode, enrichment time are set;
(5) after enrichment finishes, working electrode, contrast electrode, auxiliary electrode are put into the electrolytic cell that contains the 10ml phosphate buffered solution;
(6) measure the peak current signal.
2. the residual method of CPPU in a kind of fast detecting fruit juice according to claim 1 is characterized in that described working electrode is carbon electrode, and described contrast electrode is saturated potassium chloride electrode, and described auxiliary electrode is platinum electrode.
3. the residual method of CPPU in a kind of fast detecting fruit juice according to claim 1 is characterized in that working electrode is located on the megohmite insulant, and a circle porous mass is located at the outer ring of insulating matter.
4. the residual method of CPPU in a kind of fast detecting fruit juice according to claim 2 is characterized in that carbon electrode can freely control.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310216460.3A CN103293202B (en) | 2013-05-29 | 2013-05-29 | Method for rapidly detecting forchlorfenuron residue in fruit juice |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201310216460.3A CN103293202B (en) | 2013-05-29 | 2013-05-29 | Method for rapidly detecting forchlorfenuron residue in fruit juice |
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| Publication Number | Publication Date |
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| CN103293202A true CN103293202A (en) | 2013-09-11 |
| CN103293202B CN103293202B (en) | 2015-06-17 |
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| CN201310216460.3A Expired - Fee Related CN103293202B (en) | 2013-05-29 | 2013-05-29 | Method for rapidly detecting forchlorfenuron residue in fruit juice |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107619440A (en) * | 2017-08-10 | 2018-01-23 | 南昌大学 | A kind of CPPU artificial antigen and preparation method and the application of polyclonal antibody |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1560628A (en) * | 2004-02-23 | 2005-01-05 | �Ϻ���ͨ��ѧ | High-efficient liquid phase chromatographic analysing method for regulating pyrurine and its impurity |
| CN202066859U (en) * | 2011-05-23 | 2011-12-07 | 无锡安迪生物工程有限公司 | Rapid forchlorfenuron detection card |
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2013
- 2013-05-29 CN CN201310216460.3A patent/CN103293202B/en not_active Expired - Fee Related
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1560628A (en) * | 2004-02-23 | 2005-01-05 | �Ϻ���ͨ��ѧ | High-efficient liquid phase chromatographic analysing method for regulating pyrurine and its impurity |
| CN202066859U (en) * | 2011-05-23 | 2011-12-07 | 无锡安迪生物工程有限公司 | Rapid forchlorfenuron detection card |
Non-Patent Citations (2)
| Title |
|---|
| CELIA SUÁREZ-PANTALEÓN ET AL: ""Development and validation of a direct competitive monoclonal antibody-based immunoassay for the sensitive and selective analysis of the phytoregulator forchlorfenuron"", 《ANAL BIOANAL CHEM》, 19 April 2012 (2012-04-19), pages 2019 - 2026, XP035059642, DOI: doi:10.1007/s00216-012-5991-x * |
| 雷绍荣等: ""氯吡脲残留检测技术进展及趋势"", 《中国测试》, vol. 37, no. 6, 30 November 2011 (2011-11-30), pages 53 - 56 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107619440A (en) * | 2017-08-10 | 2018-01-23 | 南昌大学 | A kind of CPPU artificial antigen and preparation method and the application of polyclonal antibody |
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| CN103293202B (en) | 2015-06-17 |
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Granted publication date: 20150617 Termination date: 20180529 |