CN103289901B - Echinocandin B producing strain and application thereof - Google Patents
Echinocandin B producing strain and application thereof Download PDFInfo
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- CN103289901B CN103289901B CN201210041876.1A CN201210041876A CN103289901B CN 103289901 B CN103289901 B CN 103289901B CN 201210041876 A CN201210041876 A CN 201210041876A CN 103289901 B CN103289901 B CN 103289901B
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- echinocandin
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- rugulovalvus
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- 108010062092 echinocandin B Proteins 0.000 title claims abstract description 38
- 241001505555 Aspergillus rugulovalvus Species 0.000 claims abstract description 9
- 238000000855 fermentation Methods 0.000 claims description 18
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- 239000007788 liquid Substances 0.000 claims description 6
- 235000012054 meals Nutrition 0.000 claims description 5
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Abstract
The invention discloses an Echinocandin B producing strain which is a mutant strain of Aspergillus rugulovalvus ATCC 58398. The strain is collected in the General Microorganism Center of the China Committee for Culture Collection of Microorganisms and has the collection number of CGMCC (China General Microbiological Culture Collection Center) No. 5413. The invention also discloses application of the Echinocandin B producing strain. By using the obtained Echinocandin B producing strain, the yield of the prepared Echinocandin B reaches about 800 mg/l.
Description
Technical field
The invention belongs to bionic technical field, is echinocandin B producing bacterial strain and its application specifically.
Background technology
The fungal infection of serious harm human life and health in past more than the 20 years time, either mortality rate with
And the species aspect of infection is all constantly increasing, particularly in immunosuppressant patient.It is true currently used for the clinical deep for the treatment of
The key agents of bacterium infection are triazole antifungal agent thing and Amphotericin B.
Although this two classes medicine plays an important role to controlling deep fungal infections, due to these medicine tools
There is the deficiency of following three aspect, the mortality rate for making deep fungal infection disease remains high：
One is that, because the action target spot of this two classes medicine is all fungal cell membrane, its poor selectivity and causing produces larger
Toxic and side effects；
Two is due to the extensive appearance of clinical drug-resistant funguses to reduce these curative effect of medication；
Three is that these medicines are not strong to the sensitivity of the funguses such as aspergillosis and Candida albicanss, causes and is clinically difficult to control to
The disease of this kind of fungal infection.
Fragrant net class (echinocandin class) antibiotic is one group of natural product that 20 century 70s find, with identical ring
The architectural feature of shape polypeptide core and different fatty acid side chains, can suppress fungal cell wall β -1,3- Portugals to gather noncompetitive
The activity of sugared synzyme.Its unique mechanism of action, not only toxic and side effects are low, and to some azoles and Amphotericin B drug resistance
Funguses have very strong antibacterial activity, and the funguses that especially aspergillosis and Candida albicanss etc. are presented with ascendant trend have uniqueness
Curative effect, be current clinical treatment deep fungal infection " trump antibiotic ".Therefore, external big drugmaker was through more than 30 years
Research and development, successfully had listed the net class antifungal drug of three sweet smell, i.e. Caspofungin, MFG and A Nifen
Only.
Anidulafungin (Anidulafungin) is the semi-synthetic antifungal drug of third generation echinocandin class, by the U.S.
Vicuron drugmakers research and develop, after purchased by Pfizer.With traditional antifungal for acting on ergosterol in funguses serous coat
Medicine is different, and anidulafungin is one kind 1,3- callose synthetic inhibitors.1,3- callose is the weight of fungal cell wall
Ingredient, anidulafungin is wanted to make 1,3- β-D- Portugals in fungal cell wall by suppressing funguses 1,3- callose synzyme
Glycan content is reduced, and the integrity for causing cell wall is destroyed, and intracellular osmotic pressure is unstable, ultimately results in fungal cell's dissolving
It is dead.Because mammalian cell does not have cell wall, lack 1,3- callose synzyme, therefore anidulafungin has to funguses
Higher specificity, will not typically produce cytotoxicity as similar Amphotericin B, and between existing antifungal not
There is cross resistance.
Anidulafungin is obtained by carrying out side chain transformation to echinocandin B (Echinocandin B).To echinocandin B
Side chain is digested, and then connects new side chain.Echinocandin B can be by Aspergillus rugulovalvus (different titles
Aspergillus rugulosus) fermentation generation.Because the fermentation yield of echinocandin B is very low, and then anidulafungin is have impact on
Yield.Therefore, this area is in the urgent need to a kind of echinocandin B yield is high, stabilization characteristics of genetics superior strain.
The content of the invention
Present inventor is with Aspergillus rugulovalvus ATCC 58398 as starting strain, and Jing is random
One plant of echinocandin B producing bacterial strain is screened after mutation, and has been carried out preservation, preserving number is CGMCC No.5413.
Therefore, first purpose of the invention is, there is provided a kind of Aspergillus rugulovalvus, its preserving number
For CGMCC No.5413.
Second object of the present invention is, there is provided the Aspergillus rugulovalvus CGMCCNo.5413
For producing the application of echinocandin B.
Third object of the present invention is, there is provided a kind of production method of echinocandin B.
The technical problem solved required for of the invention, can be achieved through the following technical solutions：
Used as a first aspect of the present invention, a kind of echinocandin B producing bacterial strain is Aspergillus rugulovalvus
The mutant strain of the bacterial strains of ATCC 58398, the bacterial strain is preserved in China General Microbiological culture presevation administrative center, and preserving number is
CGMCC No.5413, preservation date on October 28th, 2011.
Used as a second aspect of the present invention, described bacterial strain is used to produce the application of echinocandin B.
As a third aspect of the present invention, a kind of production method of echinocandin B, it is characterised in that by right of fermenting
It is required that the Aspergillus rugulovalvus CGMCC No.5413 described in 1 obtain echinocandin B.
The condition of the fermentation is as follows：
Fermentation medium (wt)：Maltose 6.0%, dextrin 2.0%, cotton seed meal 3.0%, yeast powder 1.5%, oil 0.2%,
Glutamic acid 0.3%, KH2PO40.1%；
25 DEG C of fermentation liquid cultivation temperature, cultivates 7 days；Shaking flask rotating speed 220rpm/min.
The content of echinocandin B reaches 800mg/l or so in the fermentation liquid for obtaining by fermentation.
Beneficial effects of the present invention：The echinocandin B producing bacterial strain that the present invention is provided, stabilization characteristics of genetics, with good
Industrial applications value.The fermentation condition provided using the present invention, generation echinocandin B that can be stable.
The present invention the ultraviolet long wave mutation of starting strain Jing, ultraviolet shortwave mutation, gamma-radiation (60Co) mutation and NTG are lured
Become, by repeated screening, obtain echinocandin B producing bacterial strain, the yield of echinocandin B is up to 800mg/l or so.
Specific embodiment
Below in conjunction with specific embodiment, the invention will be further described.It should be understood that following examples are merely to illustrate this
Invention is not for restriction the scope of the present invention.
The experimental technique of unreceipted actual conditions in the following example, generally according to normal condition, or the bar that manufacturer provides
Part is carried out.
Inventor with Aspergillus rugulovalvus ATCC 58398 as starting strain, Jing (strain improvement, thing
Physicochemical mutation) one plant of echinocandin B producing bacterial strain is screened after random mutagenesises, and preservation has been carried out, specific preservation
Information is as follows：
China General Microbiological culture presevation administrative center
Yard 1, BeiChen xi Road, Chaoyang District, Beijing City institute of microbiology of Chinese Academy of Sciences postcode：100101
Preservation date：On October 28th, 2011
Deposit number CGMCC No.5413Aspergillus rugulovalvus
The mutant strain study on the stability of embodiment 1
Culture medium：
Inclined-plane/plating medium：Potato dextrose agar.
Seed culture medium (wt)：Glucose 1.0%, glycerol 1.0%, cottonseed meal 2.5%.
Fermentation medium (wt)：Maltose 6.0%, dextrin 2.0%, cotton seed meal 3.0%, yeast powder 1.5%, oil 0.2%,
Glutamic acid 0.3%, KH2PO40.1%.
Condition of culture：
28 DEG C of slant culture temperature, incubation time 5 days；
25 DEG C of seed liquor cultivation temperature, cultivates 3 days；
25 DEG C of fermentation liquid cultivation temperature, cultivates 7 days.Shaking flask rotating speed 220rpm/min.
Every 5 bottles of generation bacterial strain shake-flask culture, carries out parallel laboratory test.HPLC detects tunning, takes the average of surveyed content
Value.
Fermentation liquor treatment process：
Fermentation liquid 2ml adds 8ml methanol, vibration to soak 2 hours after mixing.Take supernatant 1ml to be centrifuged in EP pipes 12000rpm
10 minutes, supernatant carried out HPLC detections.
HPLC testing conditions are as follows：
Chromatographic column：Hypersil ODS2, C-18,4.6 × 250mm, 5 μm
Detector：Aligent 1100, DAD (210,223,260,280,276)
Detection wavelength：210nm
Column temperature：40℃
Flow velocity：1ml/min
Mobile phase：A：Water, B：Methanol
Analysis time：15min
Gradient condition is as follows：Post time=5min, are shown in Table 1.
The gradient condition of the HPLC of table 1
| Time(min) | A% | B% | FLOW |
| 0 | 25 | 75 | 1.0ml/min |
| 15 | 10 | 90 | 1.0ml/min |
| 20 | 0 | 100 | 1.0ml/min |
Testing result is shown in Table 2.
The content (mg/l) of echinocandin B in the mutant strain of the different passage numbers of table 2.
| Passage number | 1 | 2 | 3 | 4 |
| Echinocandin B content mg/l | 783 | 834 | 810 | 798 |
From table 2, the mutant strain proves hereditary stability through passing on investigation, reasonably passes on rear echinocandin B
Yield for this in peer-level.The present invention is preserved in China by the mutagenic obtained echinocandin B producing bacterial strains of starting strain Jing
General Microbiological Culture preservation administrative center, preserving number is CGMCC No.5413.
The fermentation medium that adopts of microorganism of the present invention for：Maltose 6.0%, dextrin 2.0%, cotton seed meal 3.0%, ferment
Female powder 1.5%, oil 0.2%, glutamic acid 0.3%, KH2PO40.1%, echinocandin B contains in the fermentation liquid for obtaining by fermentation
Amount reaches 800mg/l or so.
The mutant strain of embodiment 2 and the comparison of starting strain
Culture medium, condition of culture and detection method, such as embodiment 2.
Mutant strain and the comparison of starting strain, by taking echinocandin B content as an example, its comparative result such as table 3：
The mutant strain of table 3 and the comparison of starting strain
| Strain | Starting strain | Mutation mutant |
| Echinocandin B content (mg/l) | 60 | 823 |
| Relative quantity | 100% | 1372% |
| It is relative to improve multiple | - | 12.7 times |
The content of the echinocandin B of the mutant strain prepared from table 3, the present invention and the echinocandin B of starting strain
Content compare, it is relative to improve 12.7 times of multiple.
The producing bacterial strain that the present invention is provided is fermented, echinocandin B can be obtained with high yield, thereby may be ensured that Ah
The yield of nifungin, reduction anidulafungin cost.
Ultimate principle, principal character and the advantages of the present invention of the present invention has been shown and described above.The technology of the industry
Personnel it should be appreciated that the present invention is not restricted to the described embodiments, the simply explanation described in above-described embodiment and description this
The principle of invention, of the invention without departing from the spirit and scope of the present invention also to have various changes and modifications, these changes
Change and improvement is both fallen within scope of the claimed invention.The claimed scope of the invention by appending claims and its
Equivalent is defined.
Claims (4)
1. a kind of mutant strain of echinocandin B producing bacterial strain Aspergillus rugulovalvus ATCC 58398, it is special
Levy and be：The preserving number of the bacterial strain is CGMCC No.5413.
2. bacterial strain as claimed in claim 1 is used to produce the application of echinocandin B.
3. a kind of production method of echinocandin B, it is characterised in that by the Aspergillus fermented described in claim 1
Rugulovalvus CGMCC No.5413 obtain echinocandin B.
4. method as claimed in claim 3, it is characterised in that the condition of the fermentation is as follows：
Fermentation medium (wt)：Maltose 6.0%, dextrin 2.0%, cotton seed meal 3.0%, yeast powder 1.5%, oil 0.2%, paddy ammonia
0.3%, KH of acid2PO40.1%；
25 DEG C of fermentation liquid cultivation temperature, cultivates 7 days；Shaking flask rotating speed 220rpm/min.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201210041876.1A CN103289901B (en) | 2012-02-22 | 2012-02-22 | Echinocandin B producing strain and application thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201210041876.1A CN103289901B (en) | 2012-02-22 | 2012-02-22 | Echinocandin B producing strain and application thereof |
Publications (2)
| Publication Number | Publication Date |
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| CN103289901A CN103289901A (en) | 2013-09-11 |
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| CN103555591B (en) * | 2013-10-12 | 2015-06-03 | 浙江工业大学 | Method and bacterial strain for fermentation preparation of Echinocandin B |
| CN103995038A (en) * | 2014-05-28 | 2014-08-20 | 天津大学 | Method for screening echinocandin drugs through capillary electrophoresis |
| CN106497794B (en) * | 2015-09-08 | 2019-09-27 | 浙江海正药业股份有限公司 | One plant of naked born of the same parents' shell of structure nest for producing echinocandin B and its application |
| CN107779402B (en) * | 2016-08-27 | 2021-07-02 | 鲁南制药集团股份有限公司 | Screening method of echinocandin B production strain high-yield strain |
| CN114854603B (en) * | 2022-05-09 | 2023-06-27 | 中国科学院青岛生物能源与过程研究所 | Strain for high-yield echinocandin B and application thereof |
| WO2024017105A1 (en) * | 2022-07-18 | 2024-01-25 | 中国科学院青岛生物能源与过程研究所 | Transcription factor for improving yield of echinocandin compounds and use thereof |
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