CN103284685A - Device for measuring overall mechanical characteristics of eyeball of living animal and method of device - Google Patents
Device for measuring overall mechanical characteristics of eyeball of living animal and method of device Download PDFInfo
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Abstract
The invention relates to a device for measuring overall mechanical characteristics of an eyeball of a living animal, and a method of the device. The device is characterized in that a movable camera and a glass tube are fixed on a lifting support; the end of the glass tube is communicated with a hose and an infusion syringe; and a stop valve and a horizontal operating platform for injecting to the eyeball of the living animal are arranged on the infusion syringe. The method comprises the steps of communicating internal and external testing devices of the eyeball in a puncture injection manner for the eyeball of the experimental animal according to a communicator principle, and calculating to obtain the overall biomechanical characteristics of the eyeball, such as rigidity and elastic modulus by changing a liquid level of the external testing device. With the adoption of the device and the method, accurate measurement of a biomechanical characteristic carrier of the eyeball of the experimental animal is realized, the detectability of influence of ocular diseases and drugs on the biomechanical characteristics of the eyeball, and theoretical data is provided for selecting LASK (Laser Assisted In-situ Keratomi) operation parameters.
Description
Technical field
The present invention relates to a kind of measuring device and measuring method thereof of animal eyeball mechanical characteristic, particularly a kind of device and measuring method thereof of measuring the whole mechanical characteristic of living animal eyeball.
Background technology
Employed medicine when the ophthalmic diseases origin cause of formation, evolution, Drug therapys such as research glaucoma, coniform cornea, and the factors such as cutting depth of LASK when operation operation are during to the influencing of eyeball biomechanics characteristic, the whole biomechanics characteristic of eyeball is that researcher at first needs the problem that solves as mensuration such as integral rigidity, elastic modelling quantity.
Existing eyeball biomechanics characteristic is separated cornea or sclera, but after making the nonstandard test specimen of experimental facilities clamping, is tested under the condition that exsomatizes after normally animal eyeball being extractd.But because when exsomatizing test, the eyeball self structure can be damaged, and interactional factor and eyeball self structure have bigger influence to the eyeball biomechanics characteristic between life entity and the eyeball.Therefore, exist than mistake between the eyeball biomechanics characteristic that obtains of this means of testing and the actual mechanical characteristic.In addition, can damage eyeball structure when testing owing to exsomatize, therefore same eyeball only can be used for once test, cause this stripped method of testing can not be when same some pathological changes of life entity checking, drug effect or LASK operation the operation cutting depth to the influence of eyeball biomechanics characteristic.
Summary of the invention
The technical problem to be solved in the present invention is to measure the whole biomechanics characteristic of living animal eyeball, and provides a kind of for the devices and methods therefor of measuring the whole mechanical characteristic of living animal eyeball.
The present invention is achieved through the following technical solutions.
A kind of for the device of measuring the whole mechanical characteristic of living animal eyeball, comprise measuring machine lifting support, vertical survey support, video camera, horizontal fixed platform, glass tubing, flexible pipe, transfusion syringe, stop valve, levels operation platform; It is characterized in that:
On described measuring machine lifting support by the camera mount connector be fixedly connected with camera mount and setting thereof mobile camera, be fixedly connected with the vertical survey support by the measurement bracket set bolt; On the vertical survey support, by horizontal fixed platform I and horizontal fixed platform II vertical fixing glass tubing is arranged, and be communicated with flexible pipe and transfusion syringe, stop valve and levels operation platform in the termination of described glass tubing.
In technique scheme, further technical characterictic is as follows:
Described glass tubing is the insulation glass tubing, and its internal diameter is≤1.5 mm;
Described suspension labelling is the spherical crown shape structure that moves up and down with liquid level;
The syringe model of described transfusion syringe is a kind of among 5#, 6# and the 7#.
A kind ofly implement above-mentioned measuring method for the device of measuring the whole mechanical characteristic of living animal eyeball, its described method follows these steps to carry out:
(1) in glass tubing and pipeline thereof, charges into normal saline and clean, and charge into normal saline after the aerofluxus again to the certain altitude of glass tubing height;
(2) one fixation mark line is set as the video camera reference point at glass tubing, the normal saline water surface in glass tubing arranges a crown suspension labelling and floats with liquid level again, video camera is followed the tracks of liquid level suspension labelling and is recorded distance between this suspension labelling and fixation mark line, obtains the liquid level difference in change;
(3) living animal with anesthesia places on the levels operation platform also fixing, adopt tonometer to measure the intraocular pressure IOP of living animal, by the difference in height of calculating initial dropper Free water plane and eyeball center, when making the syringe needle puncture enter eyeball, it is equal with intraocular pressure that extraneous pressure keeps again;
(4) strut lower eyelid on the living animal, thrust the living animal camera oculi anterior by the infusion needle of transfusion device, utilize the distance between camera record glass tubing inner suspension labelling and fixation mark line;
(5) open the fluid stopping clip of needle tubing, distance in the startup video camera measurement glass tubing between suspension labelling and fixation mark line, carry out initial intraocular pressure place, the upwards static seepage measurement at 100mm place, 200mm place, the change in volume data of record relative pressure, glass liquid in pipe;
(6) with the speed of 200mm/min, produce pressure by the initial position 200mm oscilaltion vertical survey support that makes progress for the height of living animal eyeball, change the living animal intraocular pressure; The liquid volume of inflow or outflow eyeball is measured liquid level relative altitude variation in the glass tubing by video camera;
(7) by behind the COMPUTER CALCULATION acquisition removal effusion, the relation curve between living animal intraocular pressure and eyeball overall volume change; The eyeball stiffness coefficient computing formula that characterizes the whole mechanical characteristic of living animal eyeball is:
, and an index compares the whole mechanical characteristic of living animal eyeball with the variation of different animals model thus; The integral rigidity coefficient of living animal eyeball, the biomechanics characteristic of elastic modelling quantity are to calculate by the quantitative relationship between intraocular pressure and intraocular pressure and the eyeball change in volume.
In said method, the method that described infusion needle thrusts the living animal camera oculi anterior be with corneoscleral junction as point of puncture, the radially angle of injection needle and living animal eyeball is about 45 ° of inserting needles, the degree of depth is that needle point arrives anterior chamber's lesser ring of Merkel.
The present invention is by a kind of devices and methods therefor for the whole mechanical characteristic of measurement living animal eyeball, solved the whole biomechanics characteristic of measuring the living animal eyeball, and realized that experimental animal eyeball biomechanics characteristic carrier accurately measures, having promoted ophthalmic diseases and medicine influences power of test and the selected research means that provides of LASK surgical parameters is provided the eyeball biomechanics characteristic.
The present invention is used for measuring the device of the whole mechanical characteristic of living animal eyeball, adopts rustless steel to make, and simple in structure, mounting or dismounting are convenient, meet the health care standard; Use the method for this device, convenient and swift to living animal eyeball integral body mechanics feature measurement the time, accurately and reliably, be specially adapted to the accurate measurement of the whole mechanical characteristics of various living animal eyeballs.
Description of drawings
Fig. 1 is the whole mechanics characteristic test device of living animal eyeball of the present invention figure.
Among the figure: 1: the measuring machine lifting support; 2: the vertical survey support; 3: the measurement bracket set bolt; 4: the camera mount connector; 5: the fixation mark line; 6: the suspension labelling; 7: camera mount; 8: mobile camera; 9: horizontal fixed platform I; 10: horizontal fixed platform II; 11: glass tubing; 12: flexible pipe; 13: transfusion device; 14: stop valve; 15: the levels operation platform; 16: computer.
The specific embodiment
Below the specific embodiment of the present invention is made further instruction.
Implement a kind of for the devices and methods therefor of measuring the whole mechanical characteristic of living animal eyeball, be according to the Communicating device principle experimental animal eyeball to be passed through the puncture injection mode, the test set of the inside and outside formation of UNICOM's eyeball, and by the external test arrangements liquid level being changed the relation measure between original intraocular pressure and this eyeball change in volume and the intrinsic pressure variation, and then calculate the biomechanics characteristics such as rigidity, elastic modelling quantity of eyeball integral body.Solve the inaccuracy of existing measuring technology when measuring the eyeball biomechanics characteristic, can be used for studying pathological changes, medicine or LASK operation to the influence of eyeball biomechanics characteristic, also provide the Experiments of Machanics foundation for ophthalmic diseases and LASK operation simultaneously.
Embodiment 1
The measuring device of the whole biomechanics characteristic of a kind of living animal eyeball, this device comprises measuring machine lifting support, vertical survey support, video camera, horizontal fixed platform, glass tubing, flexible pipe, transfusion syringe, stop valve and levels operation platform; Its constituent relation is as follows:
Select the stainless steel material that meets medical requirement for use, make a horizontal survey support, vertical on described horizontal survey support a described measuring machine lifting support 1 is installed, and on measuring machine lifting support 1, is fixedly connected with camera mount 7 that can oscilaltion and the mobile camera 8 that arranges at support thereof by camera mount connector 4; Set mobile camera 8 is corresponding with the vertical glass tubing 11 that arranges on the measuring machine lifting support 1, is used for lifting changing value and the change in volume value of the normal saline liquid level in the record glass tubing 11.
On described measuring machine lifting support 1, be fixedly connected with vertical survey support 2 by measurement bracket set bolt 3, on vertical survey support 2, by horizontal fixed platform I 9 and horizontal fixed platform II 10 vertical fixing glass tubing 11 is arranged, described glass tubing 11 termination openings vertically upward, termination vertically downward is communicated with flexible pipe 12 and transfusion syringe 13 thereof, the levels operation platform 15 that also is provided with stop valve 14 and is used for being injected in the living animal eyeball on transfusion syringe 13.Measurement frame and living animal levels operation platform 15 can arrange respectively to be measured.
The above-mentioned a kind of measuring device for the whole biomechanics characteristic of living animal eyeball of the invention process, in experiment measuring, respectively living animal rabbit, Cavia porcellus and rat have been carried out using the application measurement of this device, all realized precise and stable measurement, it can be said that brightly, this device also can be applied to the measurement of the biomechanics characteristic of the whole eyeball of other living animal.
During a kind of measuring device for the whole biomechanics characteristic of living animal eyeball stated on the implementation, the glass tubing 11 that adopts is further defined to the insulation glass tubing, and its internal diameter is≤1.5 mm.In indoor temperature, carry out the measurement of living animal eyeball under the atmospheric pressure generally speaking, need not insulation; When the temperature difference is big, need be incubated, change and change in volume to guarantee the liquid that meets rule, guarantee the accuracy that the living animal eyeball is measured.
State in the scheme on the implementation, suspension labelling 6 further adopts the spherical crown shape structure that moves up and down with biological saline liquid level position, mainly be for video camera can accurately record change in volume in biological saline liquid level variation situation and the glass tubing, with the accuracy that guarantees to measure.
State on the implementation in the scheme, the transfusion syringe 13 the syringe model generally select for use among medical 5#, 6# and the 7# a kind of all can, decide on living animal eyeball situation.
Embodiment 2
Implement the above-mentioned a kind of measuring method for the measuring device of measuring the whole mechanical characteristic of living animal eyeball of the present invention, this method is to follow these steps to carry out:
Step 1, according to the health care requirement, to the measuring device disinfection, clean charging into normal saline in glass tubing 11 and the pipeline thereof, and charge into normal saline after the aerofluxus again and highly arrive the live liquid height of measurement of the whole mechanical characteristic of eye movement thing ball of required measurement to glass tubing 11, approximately be the top of selecting glass tubing 11 height for use.
Step 2, at glass tubing 11 the video camera basis reference line that a fixation mark line 5 changes as liquid level is set, the normal saline water surface in glass tubing 11 arranges a crown suspension labelling 6 and floats with liquid level again, video camera 8 is followed the tracks of liquid level suspension labellings 6 and is recorded this suspension labelling 6 and the distance of 5 on fixation mark line, obtains the liquid level difference in change.
Step 3, the living animal of anesthesia placed on the levels operation platform 15 and fixing, adopt tonometer to measure the intraocular pressure IOP of living animal, by the difference in height of calculating initial dropper Free water plane and eyeball center, when making the syringe needle puncture enter eyeball, it is equal with intraocular pressure that extraneous pressure keeps again.
Step 4, strut lower eyelid on the living animal, thrust the living animal camera oculi anterior by the infusion needle of transfusion device 13, utilize the distance of 5 on camera record glass tubing 11 inner suspension labellings 6 and fixation mark line.
Step 5, open the fluid stopping clip of needle tubing, start video camera and measure the distance of 5 of suspension labellings 6 and fixation mark line in the glass tubing 11, carry out initial intraocular pressure place, the upwards static seepage measurement at 100mm place, 200mm place, the change in volume data of record relative pressure, glass liquid in pipe;
Step 6, with the speed of 200mm/min, produce pressure by the initial position 200mm oscilaltion vertical survey support 2 that makes progress for the height of living animal eyeball, change the living animal intraocular pressure; The liquid volume of inflow or outflow eyeball is measured liquid level relative altitude variation in the glass tubings 11 by video camera 8;
Step 7, to remove effusion by COMPUTER CALCULATION after, the relation curve between living animal intraocular pressure and the variation of eyeball overall volume; The eyeball stiffness coefficient computing formula that characterizes the whole mechanical characteristic of living animal eyeball is:
, and an index compares the whole mechanical characteristic of living animal eyeball with the variation of different animals model thus; The integral rigidity coefficient of living animal eyeball, the biomechanics characteristic of elastic modelling quantity are to calculate by the quantitative relationship between intraocular pressure and intraocular pressure and the eyeball change in volume.
When the living animal eyeball is punctured, the method that infusion needle thrusts the living animal camera oculi anterior preferably with corneoscleral junction as point of puncture, it is better that the radially angle of injection needle and living animal eyeball is about 45 ° of inserting needles, and the degree of depth is that needle point arrives anterior chamber's lesser ring of Merkel the best.
The measuring method of the above-mentioned a kind of measuring device for the whole biomechanics characteristic of living animal eyeball of the invention process, respectively living animal rabbit, Cavia porcellus and rat have been carried out using the measurement of this method, measurement data is accurately reliable, and this device also can be applied to the measurement of the biomechanics characteristic of the whole eyeball of other living animal.
Be example with rabbit, Cavia porcellus and rat below, carry out the biomechanics characteristic of the whole eyeball of living animal and measure, further specify the specific embodiment of the present invention.
Specific embodiment 3
Be example with the new zealand white rabbit, before measuring, at first the environment that living animal is measured and living animal measuring appliance carry out thorough disinfection and handle, and will anaesthetizes the new zealand white rabbit of getting well and be fixed on the operation planar of levels operation platform 18 of adjustable space position, and eye speculum struts eyelid.To be full of normal saline in transfusion device 17, flexible pipe 14 and the thin glass tube 13 again.
Concrete grammar is the mode that adopts negative pressure to suck, and prevents bubble.Adjust liquid level float and experimental animal eyeball center relative altitude by the lifting arm that the lifting universal material testing reaches, make it approximate this animal statistics intraocular pressure value (about 200 mmH that reach
2O), demarcate the shooting extensometer simultaneously, make it tracking and monitoring buoy and the fixing variable in distance between the graticule, in order to flow into the liquid volume of eyeball in the computing system, obtain eyeball change in volume and pressure value.After finishing above-mentioned work, puncture to the anterior chamber near the direction of cornea one side with 45 ° of angles radially at test eyeball corneoscleral junction with the syringe needle of transfusion device, needle point rests on lesser ring of Merkel.Open stop valve, variable in distance between two labellings in the synchronous monitoring glass tubing, beginning to start shooting in the time of quantity to be measured rate of change≤1mm/s follows the tracks of, starting the test method that edits in advance afterwards tests: the static seepage test that at first is relative elemental height place, be referred to as the test of low level seepage, test continues 1 minute, record relative pressure in this process, the data such as change in volume of glass liquid in pipe, its objective is in order to obtain the seepage parameter of this system itself, in order in ensuing cyclic test, remove the seepage influence, make test data can reflect accurately more the rigidity situation of eyeball integral body then make crossbeam drive this test pipeline holder part together with slower speed by current with respect to after highly at the uniform velocity rising to new relative altitude, automatically return to original relative altitude position.Finish loop test one time, this process obtains between the interior gauge length of glass tubing apart from over time, and then obtain the exert pressure volume rule over time of rule over time and eyeball integral body of eyeball is finally calculated the biomechanical parameters such as integral rigidity of eyeball.As shown in table 1 with the biomechanics characteristic of the resulting all ages and classes rabbit eyeball of device as stated above.
Table 1. age groups lagophthalmos CCT, IOP, eyeball initial volume and stiffness coefficient are relatively
Illustrate: in the table 1, the influence of age groups eyeball initial volume be can not ignore, so definition eyeball stiffness coefficient
Specific embodiment 4
Be example with the experimental guinea pig, before measuring, at first the environment that living animal is measured and living animal measuring appliance carry out thorough disinfection and handle, and will anaesthetizes the Cavia porcellus that gets well and be fixed on the operation planar of levels operation platform 18 of adjustable space position, and eye speculum struts eyelid.To be full of normal saline in transfusion device 17, flexible pipe 14 and the thin glass tube 13 again.
Concrete grammar is the mode that adopts negative pressure to suck, and prevents bubble.Adjust liquid level float and experimental animal eyeball center relative altitude by the lifting arm that the lifting universal material testing reaches, make it approximate this animal statistics intraocular pressure value (about 150 mmH that reach
2O), demarcate the shooting extensometer simultaneously, make it tracking and monitoring buoy and the fixing variable in distance between the graticule, in order to flow into the liquid volume of eyeball in the computing system, obtain eyeball change in volume and pressure value.After finishing above-mentioned work, puncture to the anterior chamber near the direction of cornea one side with 45 ° of angles radially at test eyeball corneoscleral junction with the syringe needle of transfusion device, needle point rests on lesser ring of Merkel.Open stop valve, variable in distance between two labellings in the synchronous monitoring glass tubing, beginning to start shooting in the time of quantity to be measured rate of change≤1mm/s follows the tracks of, starting the test method that edits in advance afterwards tests: the static seepage test that at first is relative elemental height place, be referred to as the test of low level seepage, test continues 1 minute, record relative pressure in this process, the data such as change in volume of glass liquid in pipe, its objective is in order to obtain the seepage parameter of this system itself, in order in ensuing cyclic test, remove the seepage influence, make test data can reflect accurately more the rigidity situation of eyeball integral body then make crossbeam drive this test pipeline holder part together with slower speed by current with respect to after highly at the uniform velocity rising to new relative altitude, automatically return to original relative altitude position.Finish loop test one time, this process obtains between the interior gauge length of glass tubing apart from over time, and then obtain the exert pressure volume rule over time of rule over time and eyeball integral body of eyeball is finally calculated the biomechanical parameters such as integral rigidity of eyeball.As shown in table 2 with the resulting biomechanics characteristic normal and myopia group Cavia porcellus eyeball of device as stated above.
Table 2 normally reaches near-sighted Cavia porcellus eyeball diopter, axiallength and stiffness coefficient relatively
Illustrate: in the table 2, the myopia group differs very little with the normal group initial volume, so definition eyeball stiffness coefficient
Specific embodiment 5
Be example with the rat, before measuring, at first the environment that living animal is measured and living animal measuring appliance carry out thorough disinfection and handle, and will anaesthetizes the rat that gets well and be fixed on the operation planar of levels operation platform 18 of adjustable space position, and eye speculum struts eyelid.To be full of normal saline in transfusion device 17, flexible pipe 14 and the thin glass tube 13 again.
Concrete grammar is the mode that adopts negative pressure to suck, and prevents bubble.Adjust liquid level float and experimental animal eyeball center relative altitude by the lifting arm that the lifting universal material testing reaches, make it approximate this animal statistics intraocular pressure value (150 mmH that reach
2O), demarcate the shooting extensometer simultaneously, make it tracking and monitoring buoy and the fixing variable in distance between the graticule, in order to flow into the liquid volume of eyeball in the computing system, obtain eyeball change in volume and pressure value.After finishing above-mentioned work, puncture to the anterior chamber near the direction of cornea one side with 45 ° of angles radially at test eyeball corneoscleral junction with the syringe needle of transfusion device, needle point rests on lesser ring of Merkel.Open stop valve, variable in distance between two labellings in the synchronous monitoring glass tubing, beginning to start shooting in the time of quantity to be measured rate of change≤1mm/s follows the tracks of, starting the test method that edits in advance afterwards tests: the static seepage test that at first is relative elemental height place, be referred to as the test of low level seepage, test continues 1 minute, record relative pressure in this process, the data such as change in volume of glass liquid in pipe, its objective is in order to obtain the seepage parameter of this system itself, in order in ensuing cyclic test, remove the seepage influence, make test data can reflect accurately more the rigidity situation of eyeball integral body then make crossbeam drive this test pipeline holder part together with slower speed by current with respect to after highly at the uniform velocity rising to new relative altitude, automatically return to original relative altitude position.Finish loop test one time, this process obtains between the interior gauge length of glass tubing apart from over time, and then obtain the exert pressure volume rule over time of rule over time and eyeball integral body of eyeball is finally calculated the biomechanical parameters such as integral rigidity of eyeball.As shown in table 3 with the resulting biomechanics characteristic normal and the high and low pressure rat eye of device as stated above.
| ? | Normal group(n=16) | The high pressure group (n=10) | Low pressure group (n=10) |
| Stiffness coefficient (mmHg/ μ L) | 1235.58±181.95 | 1278.09±114.80 | 1108.91±171.25 |
Table 3 normally reaches the big rathole eyeball of high and low pressure stiffness coefficient relatively
Illustrate: in the table 3, the big rathole eyeball of high and low pressure is compared initial volume and is differed very little with normal group, so definition eyeball stiffness coefficient
Claims (6)
1. a device that is used for measuring the whole mechanical characteristic of living animal eyeball comprises measuring machine lifting support, vertical survey support, video camera, horizontal fixed platform, glass tubing, flexible pipe, transfusion syringe, stop valve, levels operation platform; It is characterized in that:
Be fixedly connected with camera mount (7) and the mobile camera (8) that arranges thereof, be fixedly connected with vertical survey support (2) by measurement bracket set bolt (3) by camera mount connector (4) at described measuring machine lifting support (1); By horizontal fixed platform I (9) and horizontal fixed platform II (10) vertical fixing glass tubing (11) is arranged at vertical survey support (2), and be communicated with flexible pipe (12) and transfusion syringe (13), stop valve (14) and levels operation platform (15) in the termination of described glass tubing (11).
2. as claimed in claim 1 for the device of measuring the whole mechanical characteristic of living animal eyeball, it is characterized in that: described glass tubing (11) is the insulation glass tubing, and its internal diameter is≤1.5 mm.
3. as claimed in claim 2 for the device of measuring the whole mechanical characteristic of living animal eyeball, it is characterized in that: described suspension labelling (6) is the spherical crown shape structure that moves up and down with liquid level.
4. as claimed in claim 1 for the device of measuring the whole mechanical characteristic of living animal eyeball, it is characterized in that: the syringe model of described transfusion syringe (13) is a kind of among 5#, 6# and the 7#.
5. implement the described measuring method for the device of measuring the whole mechanical characteristic of living animal eyeball of claim 1 for one kind, it is characterized in that: described method follows these steps to carry out:
(1) in glass tubing (11) and pipeline thereof, charges into normal saline and clean, and charge into the certain altitude of normal saline in the glass tubing (11) after the aerofluxus again;
(2) a fixation mark line (5) is set as the video camera reference point at glass tubing (11), the normal saline water surface in glass tubing (11) arranges a crown suspension labelling (6) and floats with liquid level again, video camera (8) is followed the tracks of liquid level suspension labelling (6) and is recorded distance between this suspension labelling (6) and fixation mark line (5), acquisition liquid level difference in change;
(3) living animal with anesthesia places levels operation platform (15) to go up and fix, measure the intraocular pressure IOP of living animal with tonometer, by the difference in height of calculating initial dropper Free water plane and eyeball center, when making the syringe needle puncture enter eyeball, extraneous pressure keeps and intraocular pressure equates again;
(4) strut lower eyelid on the living animal, thrust the living animal camera oculi anterior by the infusion needle of transfusion device (13), utilize the distance between camera record glass tubing (11) inner suspension labelling (6) and fixation mark line (5);
(5) open the fluid stopping clip of needle tubing, start the distance between the middle suspension labelling (6) of video camera measurement glass tubing (11) and fixation mark line (5), carry out initial intraocular pressure place, the upwards static seepage measurement at 100mm place, 200mm place, the change in volume data of record relative pressure, glass liquid in pipe;
(6) with the speed of 200mm/min, produce pressure by the initial position 200mm oscilaltion vertical survey support (2) that makes progress for the height of living animal eyeball, change the living animal intraocular pressure; The liquid volume of inflow or outflow eyeball is measured liquid level relative altitude variation in the glass tubing (11) by video camera (8);
(7) by behind the COMPUTER CALCULATION acquisition removal effusion, the relation curve between living animal intraocular pressure and eyeball overall volume change; The eyeball stiffness coefficient computing formula that characterizes the whole mechanical characteristic of living animal eyeball is:
, and an index compares the whole mechanical characteristic of living animal eyeball with the variation of different animals model thus; The integral rigidity coefficient of living animal eyeball, the biomechanics characteristic of elastic modelling quantity are to calculate by the quantitative relationship between intraocular pressure and intraocular pressure and the eyeball change in volume.
6. the measuring method for the device of measuring the whole mechanical characteristic of living animal eyeball as claimed in claim 6, it is characterized in that: the method that described infusion needle thrusts the living animal camera oculi anterior is as point of puncture with corneoscleral junction, the radially angle of injection needle and living animal eyeball is about 45 ° of inserting needles, and the degree of depth is that needle point arrives anterior chamber's lesser ring of Merkel.
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