CN103278581A - Method for measuring response of anoplophora chinensis to volatile substances, and trapping device - Google Patents
Method for measuring response of anoplophora chinensis to volatile substances, and trapping device Download PDFInfo
- Publication number
- CN103278581A CN103278581A CN2013102218497A CN201310221849A CN103278581A CN 103278581 A CN103278581 A CN 103278581A CN 2013102218497 A CN2013102218497 A CN 2013102218497A CN 201310221849 A CN201310221849 A CN 201310221849A CN 103278581 A CN103278581 A CN 103278581A
- Authority
- CN
- China
- Prior art keywords
- longicorn beetle
- gas chromatography
- electric potential
- longicorn
- baffle plate
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Images
Abstract
The invention discloses a method for measuring for measuring response of anoplophora chinensis to volatile substances. The method comprises the following steps of cutting tentacles of adult anoplophora chinensis along base parts; cutting a small amount of tentacle tips; horizontally putting the cut tentacles on an electrode through a conductive adhesive; introducing every reference sample into gas chromatography; carrying out gas chromatography-electroantennographic detection; repeating the experiments; carefully comparing peak shapes and retention time of compounds on chromatogram of the gas chromatography-electroantennographic detection, so as to determine active substances; further determining the active substances by wild attractive activity experiment of the anoplophora chinensis; and then screening out effective attractive chemical compound monomers according to experiment results. The active substances are obtained by directly detecting extracts of the volatile substances. Compared with previous data, the data obtained by the invention is more reliable and effective. Besides, working efficiency is increased to a large extent.
Description
Technical field
The invention belongs to the technical field of longicorn beetle research, relate in particular to and a kind ofly measure longicorn beetle to method and the trap of volatile matter.
Background technology
Longicorn beetle is Japan, the distinctive a kind of longicorn of China and Korea S, is generally all thought insect by the locality.A mating of every female longicorn beetle just can be produced and be reached 200 ovum, and every ovum all can separately be ensconced in the bark.When larva hatched, it can be chewed in the in-tree, caused a passage to be used for tying pupa.Can reach 12-18 month by laying eggs by a definite date to tying pupa and adult.Adult comes across 5 to July, lives in the level land to the intermediate altitude mountain area.Adult idol at night has photoaxis, and the worm of longicorn beetle suffers from can kill multiple different palohierror.The main biological function of longicorn beetle adult comprises gets food to supplement the nutrients, and finishes mate and oviposit to produce offspring, and shows as in behavior and seeks seeds, the searching spouse who supplements the nutrients and seek the host of laying eggs who is fit to its offspring's existence.In the process of seeking host and spouse, adult mainly is to rely on the chemocepter on the feeler to accept chemical information, and behavior reaction directed for it or that carry out next step provides signal.
At present, the relation between research plant volatile and insect adopts tentaculum electric potential technology (EAG) often, and the tentaculum electric potential technology is widely used in the entomology test, is directly to detect insect to the electric physiology method of volatile matter chemical signal reaction.But this Technology Need is at first tentatively determined target components according to document by screening, utilizes the pure product of reagent to carry out biologicall test one by one to determine that its behavior reaction to longicorn beetle is the EAG determination techniques then, and process repeats, complex operation.
The combination of tentaculum electric potential and gas chromatography is that gas chromatography tentaculum electric potential combined instrument (GC-EAD) is screening and the comparatively easy method of identifying the effecting reaction material.
The kind of insect trap has much at present, and the principle of its attracting and destroying insects and structure have different characteristics, also is applicable to insect not of the same race simultaneously, but is applicable to that the trapper of the insect that the big flight performance of this individuality of longicorn adult is stronger is less.
Summary of the invention
The purpose of the embodiment of the invention is to provide a kind of and measures longicorn beetle to method and the trap of volatile matter, be intended to solve at present domestic to process repetition in the longicorn beetle behavior reaction determination techniques, complex operation and the less problem of trapper of traping this big flight performance of longicorn beetle.
The embodiment of the invention is achieved in that a kind ofly measures longicorn beetle to the method for volatile matter, and this mensuration longicorn beetle may further comprise the steps the method for volatile matter:
The feeler of longicorn beetle adult is cut along base portion, and tip cut is after a little, with conducting resinl with its horizontal riding on the electrode;
Every kind of standard specimen sample introduction carries out the analysis of gas chromatography tentaculum electric potential combined instrument in gas chromatography, carry out repeated experiments;
Peak shape and the retention time of the compound on the gas chromatography tentaculum electric potential combined instrument chromatogram contrast in detail, thereby identify to have active material;
By the open-air attractive activity experiment of longicorn beetle is further determined, then filter out effective attractant compound monomer according to experimental result.
Further, standard specimen is respectively got 3 μ L sample introductions in gas chromatography.
Further, promoting the circulation of qi phase chromatogram tentaculum electric potential combined instrument is analyzed, repeated experiments 5 times.
Further, the chromatographic condition of gas chromatography tentaculum electric potential combined instrument is the Agilent6890N-5973N gas chromatography, and chromatographic column is DB-5MS, 60m (long) * 0.25mm (I.D.) * 0.25mm (film).
Further, the temperature programme condition of gas chromatography tentaculum electric potential combined instrument (GC-EAD) is 50 ℃ of post case initial temperatures, keeps 1min, and the heating rate with 5 ℃/min is raised to 250 ℃ then.
The purpose of the embodiment of the invention is to provide the trap of a kind of longicorn beetle, and the trap of this longicorn beetle comprises: hanging ring, conical baffled, cross baffle plate, funnel, pest catcher down;
Hanging ring is fixed on conical baffled top, conical baffled connection cross baffle plate, and cross baffle plate lower bottom part is sticked together by glass cement, and following funnel is arranged on cross baffle plate below, and pest catcher is fixed on down on the funnel,
Further, hanging ring is fixed on conical baffled top by the hole of conical plate with screw.
Further, conical baffled matching with the cross baffle plate by the ditch on the right-angled trapezium hypotenuse of cross baffle plate is connected.
Further, pest catcher is fixed on down on the funnel by thin wire.
Mensuration of the present invention longicorn beetle is to the method machine trap of volatile matter, directly utilize the extract of volatile matter to detect and obtain active component, its data with compared in the past, more reliable, effectively, the while can increase work efficiency to a great extent.The measurement result of GC-EAD of the present invention shows in the volatile chemical of China tree has 5 kinds of compounds apparent in view to the spread effect of the feeler of longicorn beetle adult, wherein Beta-firpene stimulating activity is the highest, is left-handed-alpha-firpene, laurene, butylbenzene, myrtenol secondly.We lure in the collection test in the field of China tree volatile matter to the longicorn beetle adult, adopt tygon to lure core material, by carrier itself just can be naturally slowly the release chemical substance of wherein adding, make volatile matter wherein have stable rate of release, in the long longevity of residure, can not be subjected to the influence of other weather conditions such as rain drop erosion, sunlight direct projection during use.
Description of drawings
Fig. 1 is that the mensuration longicorn beetle that provides of the embodiment of the invention is to the method flow diagram of volatile matter;
Fig. 2 is the structural representation of the longicorn beetle trap that provides of the embodiment of the invention;
Fig. 3 is that the longicorn beetle that provides of the embodiment of the invention is to the gas chromatography tentaculum electric potential combined instrument response diagram spectrogram of China tree branch volatile chemical.
Among the figure: 1, hanging ring; 2, conical baffled; 3, cross baffle plate; 4, following funnel; 5, pest catcher; 6, lure core.
Embodiment
In order to make purpose of the present invention, technical scheme and advantage clearer, below in conjunction with embodiment, the present invention is further elaborated.Should be appreciated that specific embodiment described herein only in order to explaining the present invention, and be not used in restriction the present invention.
Fig. 1 shows mensuration longicorn beetle provided by the invention to the method flow of volatile matter.For convenience of explanation, only show part related to the present invention.
Mensuration longicorn beetle of the present invention is to the method for volatile matter, and this mensuration longicorn beetle may further comprise the steps the method for volatile matter:
The feeler of longicorn beetle adult is cut along base portion, and tip cut is after a little, with conducting resinl with its horizontal riding on the electrode;
Every kind of standard specimen sample introduction carries out the analysis of gas chromatography tentaculum electric potential combined instrument in gas chromatography, carry out repeated experiments;
Peak shape and the retention time of the compound on the gas chromatography tentaculum electric potential combined instrument chromatogram contrast in detail, thereby identify to have active material;
By the open-air attractive activity experiment of longicorn beetle is further determined, then filter out effective attractant compound monomer according to experimental result.
As a prioritization scheme of the embodiment of the invention, standard specimen is respectively got 3 μ L sample introductions in gas chromatography.
As a prioritization scheme of the embodiment of the invention, promoting the circulation of qi phase chromatogram tentaculum electric potential combined instrument is analyzed, repeated experiments 5 times.
As a prioritization scheme of the embodiment of the invention, the chromatographic condition of gas chromatography tentaculum electric potential combined instrument is the Agilent6890N-5973N gas chromatography, and chromatographic column is DB-5MS, 60m (long) * 0.25mm (I.D.) * 0.25mm (film).
As a prioritization scheme of the embodiment of the invention, the temperature programme condition of gas chromatography tentaculum electric potential combined instrument (GC-EAD) is 50 ℃ of post case initial temperatures, keeps 1min, and the heating rate with 5 ℃/min is raised to 250 ℃ then.
Below in conjunction with drawings and the specific embodiments application principle of the present invention is further described.
As shown in Figure 1, the mensuration longicorn beetle of the embodiment of the invention may further comprise the steps the method for volatile matter:
S101: the feeler of longicorn beetle adult is cut along base portion, and tip cut is after a little, with conducting resinl with its horizontal riding on the electrode;
S102: every kind of standard specimen got 3 μ L sample introductions in gas chromatography, carries out gas chromatography tentaculum electric potential combined instrument GC-EAD and analyzes, and this experiment repeats 5 times;
S103: peak shape and the retention time of the compound on the gas chromatography tentaculum electric potential combined instrument GC-EAD chromatogram contrast in detail, thereby identify to have active material;
S104: by the open-air attractive activity experiment of longicorn beetle is further determined, then filter out effective attractant compound monomer according to experimental result.
The chromatographic condition of gas chromatography tentaculum electric potential combined instrument (GC-EAD) is the Agilent6890N-5973N gas chromatography, and chromatographic column is DB-5MS, 60m (long) * 0.25mm (I.D.) * 0.25mm (film); The carrier gas of gas chromatography tentaculum electric potential combined instrument (GC-EAD) is helium, and air-flow is 1ml/min; No split sampling mouth temperature: 250 ℃; The temperature programme condition of gas chromatography tentaculum electric potential combined instrument (GC-EAD) is 50 ℃ of post case initial temperatures, keeps 1min, and the heating rate with 5 ℃/min is raised to 250 ℃ then; The detecting device of gas chromatography tentaculum electric potential combined instrument (GC-EAD) is hydrogen flame detector or tentaculum electric potential detecting device; Standard specimen is sampled as 3 μ L; GC-EAD analyzes experiment and repeats 5 times.
According to the collection of illustrative plates of 5 repeated experiments, selecting one of them stimulates comparatively obviously and the collection of illustrative plates with reaction repeated, and the longicorn beetle adult is to the gas chromatography tentaculum electric potential combined instrument GC-EAD analysis result of China tree plant escaping gas, as shown in Figure 3.
The feeler of longicorn beetle adult is relatively more responsive to the spread effect of Beta-firpene, left-handed-the alpha-firpene, laurene, butylbenzene, these 5 kinds of compounds of myrtenol as shown in Figure 3, wherein Beta-firpene stimulating activity is the highest, left-handed-the alpha-firpene, laurene, butylbenzene, myrtenol can arouse slight reaction to the feeler of longicorn beetle adult, all the other compounds do not have tangible irritant reaction to feeler.
The semiochemicals of insect has tangible species specificity, and plays an important role in choice of habitat, mating location and the population distributed process of insect.Reaction is conducive to screen bioactive compound to the research semiochemicals to the gas chromatography tentaculum electric potential combined instrument GC-EAD of insect feeler, for the comprehensive regulation of insect provides effective bionomic control method.
Along with electrophysiological fast development, the combination of tentaculum electric potential and gas chromatography is that gas chromatography tentaculum electric potential combined instrument (GC-EAD) is screening and the comparatively easy method of identifying the effecting reaction material.In the past during concerning between research plant volatile and insect, at first determine target components according to document by screening is preliminary; Utilize the pure product of reagent to carry out biologicall test one by one to determine its behavior reaction to insect (EAG determination techniques) then, process repeats, complex operation, by contrast, utilize gas chromatography tentaculum electric potential combined instrument GC-EAD technology can directly utilize the extract of volatile matter to detect and obtain active component, its data with compared in the past, more reliable, effectively, can increase work efficiency to a great extent simultaneously.The measurement result of gas chromatography tentaculum electric potential combined instrument GC-EAD of the present invention shows in the volatile chemical of China tree has 5 kinds of compounds apparent in view to the spread effect of the feeler of longicorn beetle adult, wherein Beta-firpene stimulating activity is the highest, is left-handed-alpha-firpene, laurene, butylbenzene, myrtenol secondly.
Though the mensuration of tentaculum electric potential can judge comparatively accurately volatile matter to the spread effect of insect feeler with and the size of effect, can not determine what effect compound monomer plays to plant-feed insect actually.Find in test to have 5 kinds of compounds all can cause the gas chromatography tentaculum electric potential combined instrument GC-EAD reaction that longicorn beetle is stronger, but its effect that longicorn beetle adult is caused belongs to positive taxis or negative taxis, how these 5 kinds of compounds lure energy collecting power to the actual of longicorn beetle field, finally to further determine by concrete open-air biologicall test, then filter out effective attractant compound monomer according to experimental result, mix according to a certain percentage mutually again and continue to select active principle, finally determine the attractant prescription that longicorn beetle is had certain attracting action, for the control of longicorn beetle provides new technological means.
As shown in Figure 2, longicorn beetle trap of the present invention, this structure comprises hanging ring 1, conical baffled 2, cross baffle plate 3, funnel 4, pest catcher 5 down; Hanging ring 1 is fixed on conical baffled 2 tops by conical baffled 2 hole with screw, be used for to hang in the tree or longicorn beetle is lured in the place of other eminence; Conical baffled 2 match with cross baffle plate 3 by the ditch on the right-angled trapezium hypotenuse of cross baffle plate 3 is connected, and is used for preventing that excessive rainwater of rainy day from entering pest catcher the longicorn adult of collecting is gone out container; Cross baffle plate 3 lower bottom parts are sticked together by glass cement, are used for that longicorn beetle can successfully be creeped and enter down pest catcher 5; Following funnel 4 is used for making longicorn beetle fall into pest catcher accurately below cross baffle plate 3; Pest catcher 5 is fixed on down on the funnel 4 by thin wire and pours the aqueous solution of washing powder into, is used for preventing from flying out after longicorn from falling into, and is conducive to collect; Lure core to hang over cross baffle plate 3 middle parts by thin wire, be used for distributing smell and attract longicorn beetle.
Hanging ring 1 is to make with the crude iron silk, and conical baffled 2, cross baffle plate 3 and following funnel 4 all adopt the moderate iron sheet of density to make; Cross baffle plate 3 is to be made of four identical right-angled trapezium iron plates, and trapezoidal right-angle side is downward, and upper base is outside, go to the bottom and use glass cement to be sticked together, and the angle that makes every right-angled trapezium be launched into 90 ° is formed cruciform; Conical baffled 2 are slightly larger than the size of cross baffle plate, and pest catcher 5 is the 1L plastic measuring glass, pour the aqueous solution of 500ml washing powder in the pest catcher 5 into; Lure the core carrier to adopt tygon to lure core material.
Result according to the gas chromatography tentaculum electric potential combined instrument GC-EAD reaction of the mensuration of the chemical constitution of China tree escaping gas and longicorn beetle adult, we have selected the monomer of 9 kinds of volatile matter wherein to carry out the mensuration of open-air attractive activity, its purity and source see Table 1
The open-air attractive activity of table 1 longicorn beetle is tested used chemical example code name, purity and source complete list
Test method: totally 9 reagent Dan Pin, each single product arranges 2 repetitions, 2 groups of contrasts are set simultaneously, be do not add any compound trapper of the same race in contrast, respectively lure the open-air active elapsed time of core to decide on the evaporation rate of monomer, generally be 5-7 days, containing the trapper random sequence that difference lures core is arranged in the test standing forest, suspension height is 1.5m, and interval 25m makes regular check on the longicorn beetle adult quantity that lures collection to arrive between two trappers.
Results and analysis: the cross iron sheet baffle plate in the middle of cross funnel type trapper can provide place for luring the longicorn that comes to perch or creep, after in a single day longicorn falls into pest catcher 5, just can't again escape out, therefore, this trapper can be collected the longicorn of being lured substantially, while checking attractant effect, only need by checking the longicorn quantity in pest catcher 5, for the stronger insect of this flight performance of longicorn beetle, the trap effect of cross funnel type trapper is comparatively desirable, also be applicable to simultaneously the open-air trapping experiment of other large-scale insects
Each monomer field sees Table 2 to the collection effect that lures of longicorn beetle adult, in the open air in the test, 4 compounds of attractant effect have been found the longicorn beetle adult is had, be respectively beta-firpene, laurene, phellandrene and myrtenol, except these 4 kinds of compound monomers have certain attractant effect, wherein beta-firpene, laurene, phellandrene are all lured 2 of longicorn beetle adults, myrtenol is lured 1, remaining monomer is not all lured adult, does not namely show attracting action
Table 2 China tree volatile matter monomer is to the open-air attractive activity of longicorn beetle adult
Open-air attractant effect test initial stage longicorn beetle is not ideal, tentatively drawing the compound that longicorn beetle is had an open-air attractive activity comprises: the beta-firpene, laurene, phellandrene and myrtenol, the factor that influences open-air attractant effect comprises a lot, attractant monomer itself no doubt is the most key, in addition, also should be taken into account the factor of trapper, comprise profile, size, color, woodland arranges and hang, the factor of physical environment also be can not ignore, weather condition, the longicorn population quantity, the growing state of host plant and morphological feature etc. all can produce certain influence to attractant effect, not satisfactory based on open-air attractant effect before, following some suggestion is proposed:
Should fully take into account local longicorn beetle situation occurred when selecting experimental field, be arranged on the comparatively serious forest land of longicorn beetle generation under the self-sow state, get rid of the interference of human factor as far as possible, guarantee insect density;
Trapper will be prepared as early as possible, just trapper should be hung over the field ahead of time as requested before longicorn beetle sprouts wings, and guarantees the accuracy of data;
Have pertinent literature to show, the longicorn beetle adult has stronger taxis to black, therefore trapper iron sheet periphery can be covered with black or dark paster, and possible attractant effect can be better relatively;
The smell that plant discharges is the complex mixture that the volatilization secondary substance of multiple little concentration is formed, the volatile matter that plant discharges in its physiology course, be that the irritant reaction that potpourri insect that the one-tenth by the number of chemical different in kind is grouped into perceives is the synergistic result of multiple volatile matter, therefore, different compound monomers need be pressed the different proportion proportioning and form the mixed type attractant, further study its attractant effect.
Principle of work of the present invention:
A kind of longicorn beetle is that the feeler of longicorn beetle adult is cut along base portion to the realization of the gas chromatography tentaculum electric potential combined instrument GC-EAD of China tree plant escaping gas reaction and open-air lure technology, and tip cut is after a little, with conducting resinl with its horizontal riding on the electrode; Get the standard specimen sample introduction of every kind of China tree plant chemical composition in gas chromatography, carry out gas chromatography tentaculum electric potential combined instrument GC-EAD and analyze repeated experiments; Peak shape and the retention time of the compound on the gas chromatography tentaculum electric potential combined instrument GC-EAD chromatogram are contrasted in detail, thereby identify to have active material; By the open-air attractive activity experiment of longicorn beetle is further determined, then filter out effective attractant compound monomer according to experimental result.
A kind of longicorn beetle trap is to hang in the tree by hanging ring 1, longicorn beetle can be climbed downwards smoothly along cross baffle plate 3, fall in the pest catcher 5 by following funnel 4, pour the aqueous solution of 500ml washing powder in the pest catcher 5 into, can prevent from flying out after longicorn beetle from falling into, be convenient to collect longicorn beetle, conical baffled 2 are used for preventing that excessive rainwater of rainy day from entering pest catcher 5 the longicorn adult of collecting is gone out container simultaneously.
The above only is preferred embodiment of the present invention, not in order to limiting the present invention, all any modifications of doing within the spirit and principles in the present invention, is equal to and replaces and improvement etc., all should be included within protection scope of the present invention.
Claims (9)
1. measure longicorn beetle to the method for volatile matter for one kind, it is characterized in that, this mensuration longicorn beetle may further comprise the steps the method for volatile matter:
The feeler of longicorn beetle adult is cut along base portion, and tip cut is after a little, with conducting resinl with its horizontal riding on the electrode;
Every kind of standard specimen sample introduction carries out the analysis of gas chromatography tentaculum electric potential combined instrument in gas chromatography, carry out repeated experiments;
Peak shape and the retention time of the compound on the gas chromatography tentaculum electric potential combined instrument chromatogram contrast in detail, thereby identify to have active material;
By the open-air attractive activity experiment of longicorn beetle is further determined, then filter out effective attractant compound monomer according to experimental result.
2. mensuration longicorn beetle as claimed in claim 1 is characterized in that to the method for volatile matter standard specimen is respectively got 3 μ L sample introductions in gas chromatography.
3. mensuration longicorn beetle as claimed in claim 1 is characterized in that to the method for volatile matter promoting the circulation of qi phase chromatogram tentaculum electric potential combined instrument is analyzed, repeated experiments 5 times.
4. mensuration longicorn beetle as claimed in claim 1 is to the method for volatile matter, it is characterized in that, the chromatographic condition of gas chromatography tentaculum electric potential combined instrument is the Agilent6890N-5973N gas chromatography, and chromatographic column is DB-5MS, 60m (long) * 0.25mm (I.D.) * 0.25mm (film).
5. mensuration longicorn beetle as claimed in claim 1 is to the method for volatile matter, it is characterized in that, the temperature programme condition of gas chromatography tentaculum electric potential combined instrument (GC-EAD) is 50 ℃ of post case initial temperatures, keeps 1min, and the heating rate with 5 ℃/min is raised to 250 ℃ then.
6. the trap of a longicorn beetle is characterized in that, the trap of this longicorn beetle comprises: hanging ring, conical baffled, cross baffle plate, funnel, pest catcher down;
Hanging ring is fixed on conical baffled top, conical baffled connection cross baffle plate, and cross baffle plate lower bottom part is sticked together by glass cement, and following funnel is arranged on cross baffle plate below, and pest catcher is fixed on down on the funnel.
7. the trap of longicorn beetle as claimed in claim 6 is characterized in that, hanging ring is fixed on conical baffled top by the hole of conical plate with screw.
8. the trap of longicorn beetle as claimed in claim 6 is characterized in that, conical baffled matching with the cross baffle plate by the ditch on the right-angled trapezium hypotenuse of cross baffle plate is connected.
9. the trap of longicorn beetle as claimed in claim 6 is characterized in that, pest catcher is fixed on down on the funnel by thin wire.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2013102218497A CN103278581A (en) | 2013-06-06 | 2013-06-06 | Method for measuring response of anoplophora chinensis to volatile substances, and trapping device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2013102218497A CN103278581A (en) | 2013-06-06 | 2013-06-06 | Method for measuring response of anoplophora chinensis to volatile substances, and trapping device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN103278581A true CN103278581A (en) | 2013-09-04 |
Family
ID=49061161
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2013102218497A Pending CN103278581A (en) | 2013-06-06 | 2013-06-06 | Method for measuring response of anoplophora chinensis to volatile substances, and trapping device |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN103278581A (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103598048A (en) * | 2013-11-26 | 2014-02-26 | 芜湖欧标农业发展有限公司 | Anoplophora chinensis eliminating method |
| CN104965002A (en) * | 2014-12-16 | 2015-10-07 | 湖南省茶叶研究所（湖南省茶叶检测中心） | Apparatus capable of reducing background noise of electroantennographic instrument and method for detecting antennae by using same |
| CN108562668A (en) * | 2018-05-02 | 2018-09-21 | 中国农业科学院烟草研究所 | A kind of method of GC-MS and tentaculum electric potential instrument combination analysis insect infochemical |
| CN113466359A (en) * | 2021-06-04 | 2021-10-01 | 北京农业智能装备技术研究中心 | Volatile chemical pesticide spray drift pollution detection system |
-
2013
- 2013-06-06 CN CN2013102218497A patent/CN103278581A/en active Pending
Non-Patent Citations (1)
| Title |
|---|
| 李建光: "光肩星天牛对寄主植物挥发性物质的行为反应及作用机理的研究", 《中国博士学位论文全文数据库农业科技辑》 * |
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103598048A (en) * | 2013-11-26 | 2014-02-26 | 芜湖欧标农业发展有限公司 | Anoplophora chinensis eliminating method |
| CN103598048B (en) * | 2013-11-26 | 2015-09-09 | 芜湖欧标农业发展有限公司 | A kind of method eliminating longicorn beetle |
| CN104965002A (en) * | 2014-12-16 | 2015-10-07 | 湖南省茶叶研究所（湖南省茶叶检测中心） | Apparatus capable of reducing background noise of electroantennographic instrument and method for detecting antennae by using same |
| CN104965002B (en) * | 2014-12-16 | 2018-04-17 | 湖南省茶叶研究所（湖南省茶叶检测中心） | Reduce the device of tentaculum electric potential instrument background noise and the method using device detection feeler |
| CN108562668A (en) * | 2018-05-02 | 2018-09-21 | 中国农业科学院烟草研究所 | A kind of method of GC-MS and tentaculum electric potential instrument combination analysis insect infochemical |
| CN113466359A (en) * | 2021-06-04 | 2021-10-01 | 北京农业智能装备技术研究中心 | Volatile chemical pesticide spray drift pollution detection system |
| CN113466359B (en) * | 2021-06-04 | 2023-04-07 | 北京农业智能装备技术研究中心 | Volatile chemical pesticide spray drift pollution detection system |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Lucci Freitas et al. | Studies with butterfly bait traps: an overview | |
| Panthi et al. | Summer Diet and Distribution of the Red Panda(Ailurus fulgens fulgens) in Dhorpatan Hunting Reserve, Nepal | |
| CN103278581A (en) | Method for measuring response of anoplophora chinensis to volatile substances, and trapping device | |
| Krauel et al. | Brazilian free-tailed bats (Tadarida brasiliensis) adjust foraging behaviour in response to migratory moths | |
| CN104604824A (en) | Trapping device for ground arthropods | |
| Myllymäki et al. | Five‐year Surveillance of Small Mammal Abundance in Scandinavian 1 | |
| Domokos et al. | Bird communities of different woody vegetation types from the Niraj Valley, Romania | |
| Suhonen et al. | Fruit removal from rowanberry (Sorbus aucuparia) trees at urban and rural areas in Finland: a multi-scale study | |
| Hu et al. | Seasonal migration of white-backed planthopper Sogatella furcifera Horváth (Hemiptera: Delphacidae) over the Bohai Sea in northern China | |
| Quintanilla et al. | Early life trophodynamic influence on daily growth patterns of the Alboran Sea sardine (Sardina pilchardus) from two distinct nursery habitats (bays of málaga and almería) in the western Mediterranean Sea | |
| Måren et al. | Does regulated land use allow regeneration of keystone forest species in the Annapurna Conservation Area, Central Himalaya | |
| Zhou et al. | Floral visitation can enhance fitness of Helicoverpa armigera (Lepidoptera: Noctuidae) long-distance migrants | |
| Greenwood | More than a barrier: The complex effects of ecotone vegetation type on terrestrial consumer consumption of an aquatic prey resource | |
| You et al. | Biomass burning emissions contaminate winter snowfalls in urban Beijing: A case study in 2012 | |
| Underhill et al. | Sex pheromone of the clover cutworm moth, Scotogramma trifolii: Isolation, identification and field studies | |
| Casali et al. | Impact of the 2009 extreme water level variation on phytoplankton community structure in Lower Amazon floodplain lakes | |
| Reginaldo et al. | Species richness and patterns of occurrence of small non-flying mammals of Mt. Sto Tomas, Luzon Island, Philippines | |
| Takahashi et al. | Food habits of sika deer at Otome Highland, Yamanashi, with reference to Sasa nipponica | |
| Aris et al. | Diversity of Lepidoptera at REACH Biod Centre, Cameron Highlands, Malaysia | |
| CN103364309B (en) | The remote-sensing monitoring method of Wild Rhubarb population structure and the rationally amount of excavating | |
| Petrović et al. | Using pheromone traps for the detection and monitoring of the seasonal flight of the vine bud moth Theresimima ampellophaga (Bayle-Barelle, 1808)(Lepidoptera: Zygaenidae, Procridinae) in Serbia | |
| Day et al. | Host location, blood-feeding, and oviposition behavior of Culex nigripalpus (Diptera: Culicidae): their influence on St. Louis encephalitis virus transmission in southern Florida | |
| Botelho et al. | Population dynamics of Hypothenemus hampei (Coleoptera: Scolytidae) in agroforestry and monoculture systems of conilon coffee in the southern portion of the state of Amazonas, Brazil | |
| Bose et al. | Occurrence of two characteristic Wealden ferns in the Jabalpur Series | |
| Takagi | Water chemistry of headwater streams under stormflow conditions in catchments covered by evergreen broadleaved forest and by coniferous plantation |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C02 | Deemed withdrawal of patent application after publication (patent law 2001) | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20130904 |