CN103271091A - Plant bactericide containing milk protein composition - Google Patents
Plant bactericide containing milk protein composition Download PDFInfo
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Abstract
The invention discloses a plant bactericide containing a milk protein composition, belonging to the field of plant disease control. The plant bactericide consists of the following main components in percentage by mass: 0.01-6% of a milk protein composition, 1-9% of iodide, 0.1-8% of rhodanate, 2-12% of a hydrogen peroxide source material, 6-25% of an auxiliary agent and the balance of water. According to the plant bactericide, a mixture of lactoperoxidase and lactalbumin, a mixture of hydrogen peroxide and sodium percarbonate as well as a special auxiliary agent are used as important bacteriocidal substances, so that the bactericidal performance and bactericidal effect of the plant bactericide are significantly improved, and the plant bactericide has the characteristics of strong bactericidal activity, good stability, long bactericidal lasting period and strong environmental adaptability, and can meet actual requirements in the field of plant disease control.
Description
Technical field
The invention belongs to the control of plant disease field, be specifically related to a kind of plant bactericide that contains milk protein composition.
Background technology
Plant disease is a common and stubborn problem that runs in the crops production process, not only can cause the minimizing of crop yield, but also reduces quality of agricultural product.Be using of chemical pesticide at the common method of preventing and treating of plant disease.
Long-term use chemical pesticide traditional, high poison, the effect of controlling plant diseases is more and more not obvious, and resistance is more and more obvious.The medium-term and long-term simple chemical pesticide that relies on of agricultural production has destroyed the agricultural ecological structure, causes the frequent burst of damage by disease and insect and big the generation, has been absorbed in more medication and has controlled the more vicious circle of refractory.Simple dependence chemical control is returned economy, ecology and social various aspects and is brought many side effects, because the residue of pesticide problem has not only influenced agricultural product security, has also hindered the development of international trade.In recent years, novel agrochemical, biopesticide are favored by people more and more.
At present, lacto-peroxidase system is progressively used as a kind of emerging bactericide, and this system mainly comprises: lactoperoxidase, halide or class halide (except the X-, SCN-, Cl-), hydrogen peroxide (H
20
2) three parts.
Though the bactericide with lacto-peroxidase system is used to some extent in fields such as health care, food, cosmetics, water treatments, because its system to the environmental requirement height, is easily degraded, poor stability is not used in the plant sterilization field as yet to some extent.For example: publication number is to mention in the patent of CN101626779 lacto-peroxidase system is used for sterilization in the oral cavity as food additives, because temperature constant in the human body is fit to the growth of relevant enzyme system.Yet the plant growing space is open relatively, and environmental change is big, and above technology also fails to satisfy the demand of plant sterilization.Mention among the publication number CN1350428 lacto-peroxidase system is had anti-microbial effect in cosmetics, health products, because microbe species and phytopathogen difference in the cosmetics, health products are big, so can not be used for the plant sterilization field.
On the whole, lacto-peroxidase system is used the following problem that mainly faces aspect bactericide: the effective object scope of (1) bactericide is plant pathogenic microorganisms, compare with the effective object in fields such as health care, food, cosmetics, water treatment, wider, and because the long-term excessive use of chemical bactericide, make plant pathogenic microorganisms continue to be under the high-intensity selection pressure, resistance strengthens rapidly; The sterilizing ability that needs is stronger; (2) how the bactericide application places in the open air or in the greenhouse, is subjected to such environmental effects such as temperature, sunlight, humidity, wind, rainwater big; (3) stability of peroxide in the lacto-peroxidase system is poor, easily degraded.
Therefore need a kind of novel plant bactericide that contains milk protein composition, with disinfection vitality by force, good stability, the sterilization lasting period is long, environment-adapting ability is strong characteristics, satisfy the actual demand in control of plant disease field.
Summary of the invention
The present invention is according to the plant sterilization object of bactericide and the demand of germicidal applications, develop a kind of plant bactericide that contains milk protein composition, its objective is in order to overcome problem and the shortcoming that above-mentioned prior art exists, a kind of plant bactericide that contains milk protein composition is provided, to the control of plant pathogenic microorganisms, have that control efficiency is good, instant effect, lasting period are long, be difficult for characteristics affected by environment.
Technical scheme of the present invention is:
(1) a kind of plant bactericide that contains milk protein composition, constituent comprises: milk protein composition, iodide, rhodanide, hydrogen peroxide source thing, auxiliary agent and water; It is characterized in that: described milk protein composition is the composition of lactoperoxidase and lactalbumin.
With the milk protein composition of lactoperoxidase and the lactalbumin important substance as the plant disease sterilization, its lacto-peroxidase system can effectively destroy the pathogen membrane structure, lactalbumin can with microorganism metabolism in the important meals ion form the chelating body, both are in conjunction with having synergistic function, its bactericidal property is strong, bactericidal effect is good, can kill rapidly for germ spore and mycelium that site of pathological change occurs, have the advantage of repairing scab simultaneously, make site of pathological change recover normal health gradually and grow up.This plant can be used for comprising the control of plurality of plant diseases such as downy mildew, powdery mildew, anthracnose, gray mold, leaf mold, eqpidemic disease with bactericide.
(2) according to the described plant bactericide that contains milk protein composition of technical scheme (1), its constituent is as follows by mass percentage: milk protein composition 0.01-6%, iodide 1-9%, rhodanide 0.1-8%, hydrogen peroxide source thing 2-12%, auxiliary agent 6-25%, all the other compositions are water.
Each component adopts above mass percent, is at the demand of plant with fungicide effect and lasting period, carries out the suitable component proportioning, to optimize plant with the control efficiency of bactericide, prolongs the sterilization lasting period.This proportioning components is selected according to the sterilization mechanism of different albumen, can guarantee that this plant is effective Fungicidal substance (HOSCN and IO-) with the product that the bactericide reaction system generates, with the stability that solves the hydrogen peroxide source with to the adaptability of environment for use condition.
(3) according to the described plant bactericide that contains milk protein composition of technical scheme (1), it is characterized in that: described lactalbumin is one or more mixtures in newborn basic protein, beta lactoglobulin, the alpha-lactalbumin.
Breast basic protein, beta lactoglobulin, alpha-lactalbumin are antibacterial albumen important in the lactalbumin, with the metal ion of pathogenic microorganism competition metabolic requirement, stop the harmful microbe breeding, adhere to; Also can strengthen the activity of microorganism apparent motion, reduce the formation of biofilm, prevent chemical sproof generation, be combined with lactoperoxidase, effect with Synergistic, its disinfection vitality can reach more than 90%, and only uses the bactericide of lactoperoxidase, its disinfection vitality only is about 70%, and its disinfection vitality increases significantly.
(4) according to the described plant bactericide of technical scheme (1), it is characterized in that: described iodide are the composition of potassium iodide, sodium iodide, and wherein the ratio of potassium iodide and sodium iodide is: 5:2-7:1.
Potassium, sodium are the important element of plant growth, adopt potassium iodide and two kinds of combinations of sodium iodide, in the process of plant sterilization, both can replenish the important element of plant growth, can reduce production costs again, both combinations simultaneously can realize the Synergistic sterilizing effect, and its disinfection vitality is by only rising to more than 85% with 70% of potassium iodide.
(5) according to the described plant bactericide that contains milk protein composition of technical scheme (1), it is characterized in that: described rhodanide is the mixture of potassium rhodanate or potassium rhodanate and other rhodanides.
Rhodanide is by H
20
2Oxidation generates HOSCN.Since the HOSCN neutral, easier permeation cell film, and the functional group of oxidation intracellular protein suppresses the metabolism and growth system of bacterium.The main antibacterial mechanisms of OSCN-is that the oxidation protein molecule sulfydryl that comprises enzyme (SH), make the pathogen membrane structure destroyed, press and change, and the synthetic and transportation of metabolism as material is obstructed, thereby reach the effect of sterilization by Premeabilisation of cells.Potassium rhodanate can also replenish the essential potassium element of plant growth when realizing sterilizing function, have the low advantage of cost.
(6) according to the described plant bactericide that contains milk protein composition of technical scheme (1), it is characterized in that: described hydrogen peroxide source thing is the composition of hydrogen peroxide and SODIUM PERCARBONATE; Wherein, the ratio of hydrogen peroxide and SODIUM PERCARBONATE is 1:4-3:5.
The hydrogen peroxide raw material obtains easily, and use is simple, cost is low; SODIUM PERCARBONATE has environmental protection, the advantage of good stability.Both have good stability at combination, can realize the slowly-releasing of hydrogen peroxide by both appropriate proportionings, solved independent use hydrogen peroxide instability, easily decompose and have used the too high problem of SODIUM PERCARBONATE cost separately.
(7) according to the described auxiliary agent of claim 1, comprise oil matrix, stabilizing agent, dispersant, emulsifier and sticker, it is characterized in that: its constituent is as follows by mass percentage: oil matrix 5.5-15%, stabilizing agent 0.1-4%, dispersant 0.1-0.9%, emulsifier 0.2-4%, sticker 0.1-1.1%.
(8) according to the described auxiliary agent of claim 7, it is characterized in that: described oil matrix be mineral oil, glycerine in one or both compositions, described stabilizing agent is the composition of glucan and citric acid, described dispersant is one or both compositions in the poly-trisiloxanes of ethyoxyl modification, the polyether-modified polysiloxane, emulsifier is tween series, fatty alcohol-polyoxyethylene ether is a kind of or composition, and described sticker is xanthans.
Selection vegetable oil, glycerine substitute the organic matter with high poison, easy contaminated environment as oil matrix, not only can be used as the effect that the oil-in-water medium of formation and stabilizing agent also can play protection lactoperoxidase enzyme activity together; Select for use the ethyoxyl modification to gather trisiloxanes, polyether-modified polysiloxane as dispersant, can reduce the medicament surface tension, promote that soup penetrates into epidermis by pore, reduce water consumption, strengthen control efficiency by increasing bactericide with contacting of pathogen; Select for use tween series, fatty alcohol-polyoxyethylene ether as emulsifier, be conducive to form the stabilising system of wanting required for the present invention, select the nonionic emulsifier that this has specific emulsification value scope, make the formed plant of the present invention easy to use with bactericide; Xanthans can improve the adhesive ability of bactericide drop as sticker, strengthens the ability of bactericide resistance of rainwater washing against, resists series of factors such as environmental change.
The selection of auxiliary agent and attach ratios are determined to study on the basis of plant sterilization mechanism in the present invention, according to the desired enzyme stability of bactericide, each factor such as bactericide lasting period, moisture preserving time, resting period is carried out the screening of each component and the proportioning between the various composition, above composition and corresponding proportion can improve the stability of lactoperoxidase, lasting period, moisture preserving time and the resting period of prolongation bactericide, strengthen diffusion and the adhesive ability of bactericide on blade, reduce dosage.
In sum, the invention provides a kind of plant bactericide that contains milk protein composition, by multiple protein, iodide combination, improved the sterilizing ability of bactericide; Select appropriate hydrogen peroxide source thing and concentration, solved the hydrogen peroxide instability, easily the problem of degraded guarantees that bactericide discharges capacity, stabilization of peroxide in use; The composition of auxiliary agent and composition have prolonged lasting period and the moisture preserving time of bactericide, have increased the ability of opposing environmental influence, strengthen its diffusion on blade, reduce dosage.
According to verification experimental verification, control efficiency to plant disease of the present invention can reach more than 90%, lasting period can reach more than 3 weeks, control has that control efficiency is good to the plant that the present invention contains milk protein composition to plant pathogenic microorganisms, instant effect, lasting period are long, be difficult for characteristics affected by environment with bactericide, satisfies plant with the actual demand of sterilization.
Embodiment
The present invention is further illustrated by embodiment, and embodiment should not be used as limitation of the present invention.
Embodiment one
Bactericide 1: its key component is respectively by weight percentage: milk protein composition 1.6%(is lactoperoxidase 0.6% wherein, lactalbumin 1%), iodide 9%(KI7%, NaI2%), KSCN0.1%, hydrogen peroxide source thing 12%(is hydrogen peroxide 3% wherein, SODIUM PERCARBONATE 9%), oil matrix 5.5%(is vegetable oil 3.5% wherein, glycerine 2%), stabilizing agent 4%(glucan 1%, citric acid 3%), the ethyoxyl modification gathers trisiloxanes 0.1%, Tween-81 (chemical name polyoxyethylene sorbitan ether trioleate) 4%, xanthans 0.5%, all the other are water, make finished product.
Handle 1-1: with the milk protein composition in the 1.6% lactoperoxidase replacement bactericide 1, all the other materials and content are all identical, make finished product.
Handle 1-2: the clear water contrast.
Present embodiment bactericide 1 with 1000 times of clear water dilutions, is respectively applied to prevent and treat cucumber downy mildew respectively with processing 1-1, processing 1-2.
Each handles 4 repetitions.
Effect evaluation method:
Result for the treatment of is estimated with the scab repairing effect, and lesion area accounts for whole leaf area * 100% before scab repairing effect=(lesion area accounted for whole leaf area after lesion area accounted for whole leaf area-dispenser before the dispenser)/dispenser.
The drug effect computational methods:
Disease index=[Σ (the sick numbers of sheets at different levels * relative level numerical value)/investigate total number of sheets * 9] * 100;
Control efficiency (%)=[1-(disease index after disease index before the dispenser of clear water treatment region * treatment region dispenser)/(disease index before disease index after the dispenser of clear water treatment region * treatment region dispenser)] * 100
The control efficiency of a pair of cucumber downy mildew of embodiment such as following table 1 are compared with clear water, bactericide 1 and handle the expansion that 1-2 has not only effectively suppressed scab, and also it is good to have a scab repairing effect for cucumber downy mildew, instant effect, the characteristics that control efficiency is good.Bactericide 1 is compared with handling 1-1, and on the basis of basis unanimity, the plant sterilization effect of adding the bactericide 1 of milk protein composition obviously is better than only adding the processing 1-1 of lactoperoxidase, and its control efficiency reaches 90%.
From embodiment as can be known, with respect to single " lactoperoxidase ", lactoperoxidase and lactalbumin have obvious role in synergism, have significant humidification for the control efficiency of plant disease.
Table 1 cucumber downy mildew control efficiency
Embodiment two
Bactericide 2: a kind of plant bactericide that contains milk protein composition, its key component is respectively by weight percentage: milk protein composition 0.01%(wherein, lactoperoxidase 0.005%, breast basic protein 0.005%), iodide 6%(wherein, KI5%, NaI1%), KSCN 8%, and hydrogen peroxide source thing 2%(is hydrogen peroxidase 10 .75% wherein, SODIUM PERCARBONATE 1.25%), oil matrix 15%(is vegetable oil 8% wherein, glycerine 7%), stabilizing agent 2%(glucan 1%, citric acid 1%), polyether-modified polysiloxane 0.9%, Tween-81 (chemical name polyoxyethylene sorbitan ether trioleate) 0.2%, xanthans 1.1%, all the other are water, and finished product is made in each composition combination.
Handle 2-1: with the iodide that potassium iodide 6% replaces in the bactericide 2, all the other are all identical, make finished product.
Handle 2-2: the clear water contrast.
Each handles four repetitions.
Present embodiment bactericide 2 with 1000 times of clear water dilutions, is used for the control of powdery mildew of strawberry respectively with processing 2-1, processing 2-2.
Effect evaluation method reference example one.
The effect of embodiment two on powdery mildew of strawberry is as shown in table 2: with respect to handling 2-1, iodide ion with same concentrations, the bactericidal effect that adopts potassium iodide and sodium iodide composition to provide is better than only using a kind of iodide bactericidal effect, and its control efficiency can reach 96%.
Table 2 powdery mildew of strawberry control efficiency
Embodiment three
Bactericide 3: a kind of plant bactericide that contains milk protein composition, its key component is by weight percentage: milk protein composition 6%(is lactoperoxidase 4.3% wherein, breast basic protein 1.7%), iodide 1%(is KI0.75% wherein, NaI0.25%), KSCN4%, hydrogen peroxide source thing 8%(is hydrogen peroxide 1.6% wherein, SODIUM PERCARBONATE 6.4%), oil matrix 9.5%(is vegetable oil 4% wherein, glycerine 5.5%), stabilizing agent 0.1%(glucan 0.075%, citric acid 0.025%), the modification of dispersant 0.5%(ethyoxyl gathers trisiloxanes 0.25%, polyether-modified poly organo 0.25%), fatty alcohol-polyoxyethylene ether 2%, xanthans 0.1%, all the other are water.
Handle 3-1: use wherein KI0.9% of iodide 1%(, NaI0.1%) replace the iodide in the bactericide 3, all the other are all identical.
Handle 3-2: use wherein KI0.6% of iodide 1%(, NaI0.4%) replace the iodide in the bactericide 3, all the other are all identical.
Handle 3-3: the clear water contrast.
Each handles four repetitions.
The bactericide 3 of present embodiment is diluted 1000 times with clear water respectively with processing 3-1, processing 3-2, processing 3-3, be used for cladosporium leaf and fruit mould of tomato and prevent and treat.
Effect evaluation method reference example one.
Bactericide 3(KI:NaI of the present invention=3:1) with handle 3-1(KI:NaI=9:1) and handle the contrast of 3-2(KI:NaI=3:2) as can be known: scab repairing effect and the control efficiency of 3 pairs of tomato downy mildew of bactericide are best, obviously be better than handling the control efficiency of 3-1 and 3-2, hence one can see that, there is certain proper ratio scope in KI:NaI, it is optimum that its control efficiency just can reach, if surpass its scope, it is optimum that its control efficiency can not reach.
Table 3 cladosporium leaf and fruit mould of tomato control efficiency
Embodiment four
Bactericide 4: a kind of plant bactericide that contains milk protein composition, its key component is by weight percentage: milk protein composition 3%(is lactoperoxidase 1% wherein, breast basic protein 1%, beta lactoglobulin 1%), iodide 4%(is KI3.5% wherein, NaI0.5%),, KSCN2%, hydrogen peroxide source thing 4%(hydrogen peroxide 1.2%, SODIUM PERCARBONATE 2.8%), vegetable oil 7%, stabilizing agent 1%(glucan 0.6%, citric acid 0.4%), the ethyoxyl modification gathers trisiloxanes 0.3%, the composition of emulsifier 3%(Tween-81 and fatty alcohol-polyoxyethylene ether), xanthans 0.8%, all the other are water.
Handle 4-1: with the hydrogen peroxide source thing in the SODIUM PERCARBONATE 4% replacement bactericide 4, all the other are all identical.
Handle 4-2: with the hydrogen peroxide source thing in the hydrogen peroxide 4% replacement bactericide 4, all the other are all identical.
Handle 4-3: the clear water contrast.
Each handles four repetitions.
The bactericide 4 of present embodiment with 1000 times of clear water dilutions, is used for the control of pepper anthracnose respectively with processing 4-1, processing 4-2, processing 4-3.
Effect evaluation method reference example one.
Effect such as the table 4 of bactericide 4 of the present invention on pepper anthracnose, by with handle 4-1,4-2 more as can be known, be independent SODIUM PERCARBONATE or hydrogen peroxide with respect to peroxide, the composition of SODIUM PERCARBONATE and hydrogen peroxide is better for the repairing effect of plant disease spot, reach more than 96%, effective drug duration is longer, handles 1 in the lasting rising of the ability of the reparation of spray 4 pairs of scabs of medicine bactericide of the present invention, 80% rises to 94% after 24 hours after 12 hours.
Table 4 pepper anthracnose control efficiency
Embodiment five
Bactericide 5: a kind of plant bactericide that contains milk protein composition, its key component is by weight percentage: milk protein composition 4.5%(is lactoperoxidase 2.8% wherein, breast basic protein 0.6%, α-milk globulin 1.1%), iodide 3%(is KI2.2% wherein, NaI0.8%), KSCN 6%, hydrogen peroxide source thing 6%(hydrogen peroxide 2%, SODIUM PERCARBONATE 4%), glycerine 12%, stabilizing agent 3%(glucan 1.2%, citric acid 1.8%), polyether-modified polysiloxane 0.4%, fatty alcohol-polyoxyethylene ether 1%, xanthans 0.22%, all the other are water.
Handle 5-1: different with bactericide 5 is that hydrogen peroxide source thing is hydrogen peroxide source thing 6%(hydrogen peroxide 1%, SODIUM PERCARBONATE 5%).
Handle 5-2: different with bactericide 5 is that hydrogen peroxide source thing is hydrogen peroxide source thing 6%(hydrogen peroxide 4%, SODIUM PERCARBONATE 2%).
Handle 5-3: the clear water contrast.
Each handles four repetitions.
The bactericide 5 of present embodiment with 1000 times of clear water dilutions, is used for preventing and treating the control of tomato downy mildew respectively with processing 5-1, processing 5-2, processing 5-3.
Effect evaluation method reference example one.
Effect such as the table 4 of embodiment five on the tomato downy mildew, bactericide 5(hydrogen peroxide: SODIUM PERCARBONATE=1:2), handle the 5-1(hydrogen peroxide: SODIUM PERCARBONATE=1:5) and handle the 5-2(hydrogen peroxide: scab repairing effect and control efficiency that SODIUM PERCARBONATE=2:1) is handled 1 pair of tomato downy mildew as can be known preferably can reach more than 95%, obviously be better than handling the control efficiency of 5-1 and 5-2, hence one can see that, there are certain proper ratio scope in hydrogen peroxide and SODIUM PERCARBONATE, it is optimum that its control efficiency just can reach, if surpass its scope, its control efficiency has tangible reduction.
Table 5 tomato downy mildew control efficiency
Embodiment six
Bactericide 6: a kind of plant bactericide that contains milk protein composition, its key component is by weight percentage: milk protein composition 2%(is lactoperoxidase 0.8% wherein, alpha-lactalbumin 0.8, beta lactoglobulin 0.4%), iodide 8%(is KI7% wherein, NaI1%), KSCN3%, hydrogen peroxide source thing 10%(hydrogen peroxide 2.5%, SODIUM PERCARBONATE 7.5%), oil matrix 8.5%(is vegetable oil 5% wherein, glycerine 3.5%), stabilizing agent 2.5%(glucan 0.8%, citric acid 1.7%), the polyether-modified polysiloxane of dispersant 0.2%(), emulsifier 1.5%(fatty alcohol-polyoxyethylene ether), xanthans 0.3%, all the other are water.
Handle 6-1: different with bactericide 6 is that oil matrix is its mineral oil in fluid 8.5%.
Handle 6-2: different with bactericide 6 is oleaginous base not.
Handle 6-3: the clear water contrast.
Four repetitions are established in each processing.
The bactericide 6 of present embodiment with 1000 times of clear water dilutions, is used for preventing and treating the control of pepper anthracnose respectively with processing 6-1, processing 6-2, processing 6-3.
Effect evaluation method reference example one.
Six pairs of pepper anthracnoses of embodiment as table 6: the bactericide 6 that has added oil matrix with handle 6-1 and compare, its control efficiency has tangible raising, reach more than 96%, compare with processing 6-2 and handle 6-1, its reagent that the ratio that adds the mineral oil oil matrix does not add oil matrix improves to the control efficiency of disease, but improves DeGrain.Therefore, the interpolation of heterogeneity oil matrix has bigger influence to sterilization.From embodiment, vegetable oil, glycerine oil matrix the most obviously are better than mineral oil as oil matrix.
Table 6 pepper anthracnose control efficiency
Embodiment seven
Bactericide 7: a kind of plant bactericide that contains milk protein composition, its key component is by weight percentage: milk protein composition 5%(is lactoperoxidase 2.3% wherein, breast basic protein 2.7%), iodide 2%(is KI1.5% wherein, NaI0.5%), KSC 5%, hydrogen peroxide source thing 9%(hydrogen peroxide 2.25%, SODIUM PERCARBONATE 6.75%), oil matrix 13.5%(is vegetable oil 6.5% wherein, glycerine 7%), stabilizing agent 1.5 %(glucans 0.4%, citric acid 1.1%), the polyether-modified polysiloxane 0.7% of dispersant, the composition of emulsifier 2.6%(Tween-81 and fatty alcohol-polyoxyethylene ether), xanthans 0.4%, all the other are water.
Handle 7-1: different with bactericide 7 is that dispersant 0.7% is sodium lignin sulfonate.
Handle 7-2: different with bactericide 7 is not contain dispersant.
Handle 7-3: the clear water contrast.
Four repetitions are established in each processing.
The bactericide 7 of present embodiment with 1000 times of clear water dilutions, is used for the control of cucumber downy mildew respectively with processing 7-1, processing 7-2, processing 7-3.
Effect evaluation method reference example one.
As shown in table 7: that bactericide 7 is compared with processing 7-1, processing 7-2, contain polyether-modified polysiloxane and obviously be better than handling 7-1 as the bactericide 7 of dispersant, be more conducive to bactericide and play a role, water-saving result is good, mu water consumption Jie Keda 10kg.Compare with non-dispersant processing 7-2, its every mu water-saving amount reaches 20kg.
Table 7 cucumber downy mildew control efficiency
Embodiment eight
Bactericide 8: a kind of plant bactericide that contains milk protein composition, its key component is by weight percentage: milk protein composition 1%(is lactoperoxidase 0.3% wherein, breast basic protein 0.4, beta lactoglobulin 0.2%, alpha-lactalbumin 0.1%), iodide 5%(is KI4% wherein, NaI1%), KSCN 1%, hydrogen peroxide source thing 3%(hydrogen peroxidase 10 .84%, SODIUM PERCARBONATE 2.16%), oil matrix 10.5%(is vegetable oil 4.2% wherein, glycerine 6.3%), stabilizing agent 3.2%(glucan 1.8%, citric acid 1.4%), the modification of dispersant 0.6%(ethyoxyl gathers trisiloxanes 0.2%, polyether-modified polysiloxane 0.4%), emulsifier 3.4%(fatty alcohol-polyoxyethylene ether 2.2%, Tween-81 1.2%), xanthans 0.7%, all the other are water.
Handle 8-1: different with bactericide 8 is that emulsifier 3.4% is polyoxyethylene cetyl alcohol.
Handle 8-2: different with bactericide 8 is not contain emulsifier.
Handle 8-3: the clear water contrast.
Four repetitions are established in each processing.
The bactericide 8 of present embodiment with 1000 times of clear water dilutions, is used for the control of graw mold of tomato respectively with processing 8-1, processing 8-2, processing 8-3.
Effect evaluation method reference example one.
As shown in table 8: bactericide 8 is compared with processing 8-1, processing 8-2, and the mixture of selecting fatty alcohol-polyoxyethylene ether and Tween-81 for use is that the bactericide 8 of emulsifier obviously is better than handling 8-1, handles 8-2, is more conducive to bactericide and plays a role.
By handle 1 with handle 2, handle 3 relatively, handle emulsifier in 1 and more be conducive to bactericide and play a role.
Table 8 graw mold of tomato control efficiency
Embodiment nine
Bactericide 9: a kind of plant bactericide that contains milk protein composition, its key component is by weight percentage: milk protein composition 2.4%(is lactoperoxidase 1.5% wherein, beta lactoglobulin 0.9%), iodide 7%(KI5%, NaI2%), KSCN7%, hydrogen peroxide source thing 5%(hydrogen peroxide 1.6%, SODIUM PERCARBONATE 3.4%), oil matrix 6.8%(is vegetable oil 3.4% wherein, glycerine 3.4%), stabilizing agent 0.6 %(glucan 0.42%, citric acid 0.18%), the modification of dispersant 0.46%(ethyoxyl gathers trisiloxanes 0.2%, polyether-modified polysiloxane 0.26%), emulsifier fatty alcohol-polyoxyethylene ether 0.8%, sticker xanthans 0.2%, all the other are water.
Handle 9-1: different with bactericide 9 is to replace xanthans with gum Arabic.
Handle 9-2: different with bactericide 9 is not contain xanthans.
Handle 9-3: the clear water contrast
Four repetitions are established in each processing.
The bactericide 9 of present embodiment with 1000 times of clear water dilutions, is used for the control of cladosporium leaf and fruit mould of tomato respectively with processing 9-1, processing 9-2, processing 9-3.
Effect evaluation method reference example one.
As shown in table 9: as bactericide 9 to be compared with processing 9-1, processing 9-2, selected for use xanthans obviously to be better than handling 9-1, to handle 9-2 as the bactericide 9 of sticker, more be conducive to bactericide performance bactericidal effect.
Table 9 cladosporium leaf and fruit mould of tomato control efficiency
Embodiment ten
Bactericide 10: a kind of plant bactericide that contains milk protein composition, its key component is by weight percentage: milk protein composition 3.8%(is lactoperoxidase 1.5% wherein, breast basic protein 1%, beta lactoglobulin 0.5%, alpha-lactalbumin 0.8%), iodide 5.6%(is KI 4.0% wherein, NaI 1.6%), KSCN 1.5%, hydrogen peroxide source thing 7%(hydrogen peroxide 2.38%, SODIUM PERCARBONATE 4.62%), oil matrix 12.8%(is vegetable oil 10% wherein, glycerine 2.8%), stabilizing agent 3.6%(glucan 2.5%, citric acid 1.1%), the poly-trisiloxanes 2.4% of dispersant 0.8%(ethyoxyl modification, polyether-modified polysiloxane 1.1%), emulsifier fatty alcohol-polyoxyethylene ether 1.9%, sticker xanthans 0.9%, all the other are water.
Handle 10-1: different with bactericide 10 is not oleaginous base, dispersant, emulsifier, sticker.
Handle 10-2: the clear water contrast.
Four repetitions are established in each processing.
The bactericide 10 of present embodiment with 1000 times of clear water dilutions, is used for the control of early blight of tomato respectively with processing 10-1, processing 10-2.
Effect evaluation method reference example one.
As shown in table 10: that bactericide 10 is compared with processing 10-1, processing 10-2, the bactericide 10 easier effects with main sterilization component in the reagent that contain the auxiliary component of oil matrix, dispersant, emulsifier, sticker are brought into play, the effect that significantly is better than not containing the processing 10-1 of auxiliary agent for the control efficiency of early blight of tomato, simultaneously, its moisture preserving time obviously prolongs.
Table 10 early blight of tomato control efficiency
Embodiment 11
Bactericide 11: a kind of plant bactericide that contains milk protein composition, its key component is by weight percentage: milk protein composition 5.4%(is lactoperoxidase 2.1% wherein, breast basic protein 0.9%, beta lactoglobulin 1.3%, alpha-lactalbumin 1.1%), iodide 6.5%(is KI5.33% wherein, NaI1.17%), KSCN 0.5%, hydrogen peroxide source thing 8.5%(hydrogen peroxide 2.72%, SODIUM PERCARBONATE 5.78%), oil matrix 6.6%(is vegetable oil 3% wherein, glycerine 3.6%), stabilizing agent 2.9%(glucan 0.8%, citric acid 1.4%), the modification of dispersant 0.36%(ethyoxyl gathers trisiloxanes 0.19%, polyether-modified polysiloxane 0.17%), emulsifier fatty alcohol-polyoxyethylene ether 0.7%, sticker xanthans 0.7%, all the other are water.
Handle 11-1: prior art chemical bactericide A.
Handle 11-2: the clear water contrast.
Four repetitions are established in each processing.
With the bactericide 11 of present embodiment with handle 11-1, handle 11-2 respectively with 1000 times of clear water dilutions, be used for the control of cladosporium leaf and fruit mould of tomato, in the growth season of tomato respectively with 7 days/time, 14 days/time with sprayed on cladosporium leaf and fruit mould of tomato in 21 days/time.
Effect evaluation method reference example one.
Control efficiency such as following table 11 to cladosporium leaf and fruit mould of tomato.The control efficiency of 11 pairs of cladosporium leaf and fruit mould of tomato of bactericide is compared with existing chemical sterilization technology, and control efficiency is good, and the lasting period is long, even 21 days/time frequency, its control efficiency still reaches 86%, be higher than far away existing chemical sterilization technology control efficiency 30%.
The different dispenser frequencies of table 11 are to the control efficiency of cladosporium leaf and fruit mould of tomato
Embodiment 12
Bactericide 12: a kind of plant bactericide that contains milk protein composition, its key component is by weight percentage: milk protein composition 0.7%(is lactoperoxidase 0.2% wherein, alpha-lactalbumin 0.5%), iodide 2.5%(is KI2% wherein, NaI0.5%), KSCN3.5%, hydrogen peroxide source thing 11%(hydrogen peroxide 3%, SODIUM PERCARBONATE 8%), oil matrix 7.6%(is vegetable oil 5.3% wherein, glycerine 2.3%), stabilizing agent 0.2 %(glucan 0.05%, citric acid 0.15%), the modification of dispersant 0.24%(ethyoxyl gathers trisiloxanes 0.08%, polyether-modified polysiloxane 0.16%), emulsifier fatty alcohol-polyoxyethylene ether 3.8%, sticker xanthans 0.7%, all the other are water.
Handle 12-1: with bactericide 12 different be not oleaginous base and stabilizing agent.
The enzyme activity preservation degree of results of regular determination lactoperoxidase.
The vigor preservation degree of lactoperoxidase the results are shown in Table 12, and bactericide 12 is compared with processing 12-1: oil matrix and stabilizing agent are conducive to preserve the vigor of lactoperoxidase, and enzyme activity degree of preservation is increased to 90% by 65%.
The vigor preservation degree of table 12 lactoperoxidase
Claims (8)
1. plant bactericide that contains milk protein composition, constituent comprises: milk protein composition, halide, rhodanide, hydrogen peroxide source thing, auxiliary agent and water; It is characterized in that: described milk protein composition is the composition of lactoperoxidase and lactalbumin.
2. want 1 described plant bactericide according to right, it is characterized in that: its constituent is as follows by mass percentage: milk protein composition 0.01-6%, iodide 1-9%, rhodanide 0.1-8%, hydrogen peroxide source thing 2-12%, auxiliary agent 6-25%, all the other compositions are water.
3. according to the described plant bactericide of claim 1, it is characterized in that: described lactalbumin is one or more mixtures in newborn basic protein, beta lactoglobulin, the alpha-lactalbumin.
4. according to the described plant bactericide of claim 1, it is characterized in that: described iodide are the composition of potassium iodide, sodium iodide, and wherein the ratio of potassium iodide and sodium iodide is: 5:2-7:1.
5. according to the described plant bactericide of claim 1, it is characterized in that: described rhodanide is the mixture of potassium rhodanate or potassium rhodanate and other rhodanides.
6. according to the described plant bactericide of claim 1, it is characterized in that: described hydrogen peroxide source thing is the composition of hydrogen peroxide and SODIUM PERCARBONATE, and wherein the ratio of hydrogen peroxide and SODIUM PERCARBONATE is 1:4-3:5.
7. according to the described auxiliary agent of claim 1, comprise oil matrix, dispersant, emulsifier and sticker, it is characterized in that: its constituent is as follows by mass percentage: oil matrix 5.5-15%, stabilizing agent 0.1-4%, dispersant 0.1-0.9%, emulsifier 0.2-4%, sticker 0.1-1.1%.
8. according to the described auxiliary agent of claim 7, it is characterized in that: described oil matrix be vegetable oil, glycerine in one or both compositions, described stabilizing agent is the composition of glucan and citric acid, described dispersant is one or both compositions in the poly-trisiloxanes of ethyoxyl modification, the polyether-modified polysiloxane, emulsifier is tween series, fatty alcohol-polyoxyethylene ether is a kind of or composition, and described sticker is xanthans.
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