CN103267757A - Method for determining deacetylation degree of chitosan fibers - Google Patents
Method for determining deacetylation degree of chitosan fibers Download PDFInfo
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- CN103267757A CN103267757A CN2013101674878A CN201310167487A CN103267757A CN 103267757 A CN103267757 A CN 103267757A CN 2013101674878 A CN2013101674878 A CN 2013101674878A CN 201310167487 A CN201310167487 A CN 201310167487A CN 103267757 A CN103267757 A CN 103267757A
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
- G01N21/77—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator
- G01N21/78—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator producing a change of colour
Abstract
The invention provides a method for determining the deacetylation degree of chitosan fibers in a formula mode. The deacetylation degree of chitosan fibers is obtained through calculating according to the following formula: D.D=[0.2032*C*f*(V1-V2)]/[m2*(m1/m)+0.0420*C*f*(V1-V2)]*100%. The method for determining the deacetylation degree of chitosan fibers has the advantages of very small error, high sensitivity, simplicity, and accuracy.
Description
Technical field
The present invention relates to a kind of method of measuring the chitin fiber deacetylation, particularly relate to a kind of method with colloid titration chitin fiber deacetylation.
Background technology
Shitosan is the product of chitin N-deacetylation, and chitin fiber is that raw material makes by wet spinning with the pure chitosan, that is to say that chitin fiber and shitosan are the different same a kind of materials of physical form.Chitin fiber is a kind of linear polymer, has characteristics such as biological safety, broad-spectrum antibacterial, biocompatibility, biodegradability, also has the effect of absorption chelating, dispelling abnormal flavor, hemostasia and healing and promotion skin microecological balance.Obtain application more and more widely in recent years in fields such as space flight and aviation, military products, tobacco, health care, military textiless for general use.As everyone knows, the chitin fiber deacetylation is more high, more is conducive to attaching and the growth of cell, more good with the compatibility of cell, and along with the increase of chitin fiber deacetylation, its biocidal property strengthens thereupon, therefore, deacetylation becomes the most important index of judgement chitin fiber service quality.
For these reasons, China's grade of also dividing the chitin fiber product according to the height of the deacetylation of chitin fiber.Therefore, how easy, measure the deacetylation of chitin fiber fast and accurately, become a vital ring of judging chitin fiber quality quality.
At present, it is many according to alkalimetry mensuration deacetylation in the marine industry standard SC/T3403-2004 of the People's Republic of China (PRC) " chitin and the shitosan " appendix A to measure the chitin fiber deacetylation, be about to dissolve in the chitin fiber sample adding hydrochloric acid solution, amino in the shitosan and the hydrogen ion in the hydrochloric acid solution also have remaining hydrogen ion after the combination fully, splash into remaining hydrogen ion generation neutralization reaction in a certain amount of sodium hydroxide solution and the solution, after complete reaction, reach titration end-point, with methyl orange-aniline blue as titration endpoint indicator, solution becomes light green color by aubergine, and calculates.But the assay method of above-mentioned national aquatic products portion industry standard has following defective:
1, when the concentration of hydrochloric acid increased, the result who records reduced thereupon, made that the result of test is inaccurate.
2, only be suitable for and dissolve the solvent of indivedual inorganic acid solutions of chitin fiber fully, the most frequently used can not dissolve the acetum of chitin fiber and the solvent of other organic acid soln fully and be suitable for.
Summary of the invention:
The purpose of this invention is to provide a kind of method of measuring the chitin fiber deacetylation, set up a kind of have high sensitivity, easy, accurately measure the method for chitin fiber deacetylation.
Theoretical foundation of the present invention is: colloid titration method is a kind of volumetric analysis of measuring polyelectrolyte charged group in the aqueous solution.Shitosan dissolves in diluted acid, and the lotus that becomes positively charged forms positively charged polyelectrolyte in conjunction with acid molecule or in conjunction with 1 H+ on amino.
When polyelectrolyte runs into the polyelectrolyte that has opposite charges in aqueous solution, can form neutral associated matter with certain stoichiometric relationship generation charge neutrality reaction each other.
Utilize the known charged polyelectrolyte of chemical constitution, molecular weight and concentration to measure the charged group quantity of shitosan, the theoretical foundation of the amino content of Here it is colloid titration shitosan is judged the dropping point terminal point with ocular estimate.
(Potassium Polyvinyl Sulfate PVSK) as titrant, is indicator with positively charged blue organic dyestuff toluidine blue to anionic polyelectrolyte polyvinyl sulfuric acid potassium in the method.Utilize toluidine blue (Toluidine Blue, T.B) be because it is positively charged for indicator, in acid solution, do not react with polyelectrolyte shitosan generation charge neutrality, but it can be combined with electronegative polyvinyl sulfuric acid potassium, but, this combination lags behind the reaction between the positive and negative polyelectrolyte.Before titration end-point, solution is blue, when reaching home, excessive polyvinyl sulfuric acid potassium reacts with toluidine blue immediately, thereby makes solution become aubergine by blueness, and generates flocculent deposit, because color transition is very sensitive, so can accurately judge the terminal point of titration.
Based on above-mentioned theory and purpose of the present invention, the technical solution adopted in the present invention is:
1, a kind of method of measuring the chitin fiber deacetylation is characterized in that, may further comprise the steps:
Step 1: the chain link relative molecular mass of the preparation of PVSK solution: PVSK is with C
2H
3OSO
3K is calculated as 162.2040, takes by weighing a certain amount of PVSK, is made into the solution that amount of substance concentration is C mol/L (generally the amount of substance concentration of using is 0.0025mol/L or 0.0050mol/L) with deionized water; Demarcate with titer again, determine the factor value of this liquid, i.e. correction factor f.
Step 2: the preparation of T.B indicator: take by weighing 0.1000g T.B, be made into the solution of 100.0000g with deionized water, its solute massfraction is 0.1%.
Step 3: taking by weighing constant weight is m
1Gram is (in the experiment, be generally less than 1 gram) chitin fiber to be measured, it is positioned in the conical flask, the acid solution (the solute massfraction generally is not higher than 5%) that adds the different solute massfractions that are fit to the dissolving chitin fiber, be mixed with the chitosan solution m gram (the solute massfraction generally is not higher than 2%) of different solute massfractions, be stirred to fully dissolving, treat bubble collapse after, accurate weighing m
2Gram (in the experiment, being generally less than 1 gram) solution adds the 50ml deionized water in conical flask, splash into 3～4 T.B indicator; While stirring the PVSK vs is slowly splashed on magnetic stirring apparatus, solution becomes aubergine by blueness, and colour-fast and flocky precipitate occurs in 30s, is titration end-point, reads the titration volume V of polyvinyl sulfuric acid potassium vs
1Ml.
In splashing into polyvinyl sulfuric acid potassium vs process, rate of titration is preferably controlled at 0.03ml/s, if rate of titration is too fast, tends to occur temporary transient titration end-point phenomenon.
Step 4: get with step 3 in the deionized water of equal number, carry out blank titration by above-mentioned same method, read the titration volume V of polyvinyl sulfuric acid potassium vs
2Ml;
Step 5: the deacetylation that is calculated as follows chitin fiber:
M
1＝161.1558×C×0.001×f×(V
1-V
2)
M
2＝m
2×(m
1/m)-M
1
D·D＝(M
1/161.1558)/(M
1/161.1558+M
2/203.1925)×100％
With M
1And M
2Substitution:
D·D＝{【161.1558×C×0.001×f×(V
1-V
2)】/161.1558}/{【161.1558×C×0.001×f×(V
1-V
2)】/161.1558+【m
2×(m
1/m)-161.1558×C×0.001×f×(V
1-V
2)】/203.1925}×100％
Calculate at last:
D·D＝【0.2032×C×f×(V
1-V
2)】/【m
2×(m
1/m)+0.0420×C×f×(V
1-V
2)】×100％
Technique effect of the present invention is: technical scheme of the present invention provides a kind of method of measuring the chitin fiber deacetylation with the mode of formula, and error is very little, set up a kind of have high sensitivity, easy, accurately measure the method for chitin fiber deacetylation.
For technique effect of the present invention is described, the colloid titration method of current series invention is measured the deacetylation alkalimetry with alkalimetry in the marine industry standard SC/T3403-2004 of the People's Republic of China (PRC) " chitin and the shitosan " appendix A and is the contrast experiment:
Getting constant weight is 0.3000g, deacetylation be 90% chitin fiber to be dissolved in the solute massfraction respectively be in 1%, 2%, 5% the acetum, be mixed with 30g solute quality and be 1% chitosan solution, adopt colloid titration;
Getting constant weight is 0.3000g, deacetylation is 90% chitin fiber, being dissolved in 30ml amount of substance concentration respectively is in the hydrochloric acid solution of 0.10mol/L, 0.15mol/L and 0.20mol/L, and the weight that takes by weighing the chitosan solution that is mixed with respectively is 30.2997g, 30.3000g, 30.2998g, adopts colloid titration method and alkalimetry to measure;
More than two kinds of methods, the result who records contrasts as follows:
By above data as can be seen:
1, the result of colloid titration of the present invention is more accurate than the result of alkalimetry mensuration.The average error as a result of colloid titration of the present invention is respectively 0.01%, 0.02%, and the average error as a result that alkalimetry is measured is 1.66%.
2, the result of colloid titration of the present invention is irrelevant with the concentration of the acid solution that can dissolve chitin fiber fully, thereby can avoid lack of standardization owing to operation or the result of the chitin fiber deacetylation of the solvent strength variable effect mensuration that error causes.
3, the result of colloid titration of the present invention is irrelevant with the kind of the acid solution that dissolves chitin fiber fully, just do not having under the situation of hydrochloric acid solution, can with multiple acid solutions such as acetic acid, break through the unique limitation of alkalimetry solvent, bring convenience for mensuration chitin fiber deacetylation.
4, viscosity is one of important indicator of shitosan, viscosity measurement is to carry out according to the marine industry standard SC/T3403-2004 of the People's Republic of China (PRC) " chitin and shitosan " 6.5, stipulate in the standard that using the solute massfraction is that to be mixed with the solute massfraction be 1% chitosan solution for 1% acetum.According to above-mentioned standard code, measure deacetylation and the viscosity of shitosan and need prepare two kinds of solution; And adopt the colloidal titration rule only need prepare a kind of solution, and namely according to colloid titration method, get the solution of a certain amount of mensuration shitosan viscosity, measure the deacetylation of shitosan.This shows, when measuring the index of shitosan, adopt colloid titration method than alkalimetry high efficiency.
The reason of above difference mainly is: to be chitin fiber dissolve in the acid solution of the different solute massfractions that are fit to the dissolving chitin fiber colloid titration method, on amino in conjunction with acid molecule or in conjunction with 1 H
+And the lotus that becomes positively charged forms positively charged polyelectrolyte and electronegative polyelectrolyte PVSK, and one to one the charge neutrality reaction takes place, and is the number of the chitosan molecule that directly records, and the result who records is accurate; And being chitin fiber, alkalimetry in hydrochloric acid solution, dissolves H in the amino on the chitin fiber and the watery hydrochloric acid
+After the combination, also has remaining H fully
+, splash in a certain amount of NaOH and the solution and remain H
+The generation neutralization reaction, be remaining H in the solution that records
+Number, by asking poor, shift onto the number of the chitosan molecule that obtains, cause the result that deviation is arranged, in addition, NaOH can be more or less with chitin fiber amino on the H of combination
+React, cause the consumption of NaOH to increase, cause the result who finally records on the low side.
Label declaration in the instructions:
M
1---the fibre weight of chitin fiber deacetylate in the determinand, unit is gram (g);
M
2---the chitin fiber fibre weight of deacetylate not in the determinand, unit is gram (g);
C---the concentration of the amount of polyvinyl sulfuric acid potassium vs, unit is every liter of mole (mol/L);
0.001ml with the conversion relation of L, i.e. 1ml=0.001L;
F---the correction factor of polyvinyl sulfuric acid potassium vs;
V
1---the titer of polyvinyl sulfuric acid potassium vs in the step 3, unit are milliliter (ml);
V
2---the titer of polyvinyl sulfuric acid potassium vs in the step 4, unit are milliliter (ml);
The weight of m---the chitosan solution prepared in the step 3, unit is gram (g);
m
1---the constant weight weight of the chitin fiber to be measured that takes by weighing in the step 3, unit is gram (g);
m
2---the weight of the chitosan solution that takes by weighing in the step 3, unit is gram (g);
161.1558---the relative molecular mass of a sugar unit in the shitosan;
203.1925---the relative molecular mass of a sugar unit in the chitin;
The deacetylation of DD---chitin fiber, unit are percent (%)
Embodiment
Now a kind of method of measuring the chitin fiber deacetylation provided by the invention is elaborated:
Embodiment one:
Take by weighing 0.4220g PVSK, be made into the solution of 1000ml with deionized water.Concentration is 0.0025mol/L, demarcates with titer, and the f that determines this liquid is 1.02.
Take by weighing 0.1000g T.B, be made into the solution of 100.0000g with deionized water, its solute massfraction is 0.1%.
Take by weighing deacetylation and be 90% constant weight chitin fiber m
1(1.0000) g shreds it in conical flask that is positioned over 150ml, and adding solute massfraction is 1% acetum, to m (100.0000) g; Dissolve fully and the complete back of deaeration weighing m
2(1.0000) g solution adds the 50ml deionized water in conical flask, splashes into 3～4 T.B indicator; While stirring the PVSK vs is slowly splashed on magnetic stirring apparatus, solution becomes aubergine by blueness, and colour-fast and flocky precipitate occurs in 30s, is titration end-point, reads the titration volume V of polyvinyl sulfuric acid potassium vs
1Be 21.49ml.
Get the 50ml deionized water, carry out blank titration by above-mentioned same method, and read the titration volume V of polyvinyl sulfuric acid potassium vs
2Be 0.15ml;
Be calculated as follows the deacetylation of chitin fiber:
D·D＝【0.2032×C×f×(V
1-V
2)】/【m
2×(m
1/m)+0.0420×C×f×(V
1-V
2)】×100％
＝【0.2032×0.0025×1.02×(21.49-0.15)】/【1.0000×?(1.0000/100.0000)+0.0420×0.0025×1.02×(21.49-0.15)】×100％
＝90.00％
Embodiment two:
Take by weighing 0.4220g PVSK, be made into the solution of 1000ml with deionized water.Concentration is 0.0025mol/L, demarcates with titer, and the f that determines this liquid is 1.02.
Take by weighing 0.1000g T.B, be made into the solution of 100.0000g with deionized water, its solute massfraction is 0.1%.
Take by weighing deacetylation and be 90% constant weight chitin fiber m
1(1.0000) g shreds it in conical flask that is positioned over 150ml, and adding solute massfraction is 2% acetum, to m (100.0000) g; Dissolve fully and the complete back of deaeration weighing m
2(1.0000) g solution adds the 50ml deionized water in conical flask, splashes into 3～4 T.B indicator; While stirring the PVSK vs is slowly splashed on magnetic stirring apparatus, solution becomes aubergine by blueness, and colour-fast and flocky precipitate occurs in 30s, is titration end-point, reads the titration volume V of polyvinyl sulfuric acid potassium vs
1Be 21.47ml.
Get the 50ml deionized water, carry out blank titration by above-mentioned same method, and read the titration volume V of polyvinyl sulfuric acid potassium vs
2Be 0.15ml;
Be calculated as follows the deacetylation of chitin fiber:
D·D＝【0.2032×C×f×(V
1-V
2)】/【m
2×(m
1/m)+0.0420×C×f×(V
1-V
2)】×100％
＝【0.2032×0.0025×1.02×(21.47-0.15)】/【1.0000×(1.0000/100.0000)+0.0420×0.0025×1.02×(21.47-0.15)】×100％
＝89.94％
Embodiment three:
Take by weighing 0.4220g PVSK, be made into the solution of 1000ml with deionized water.Concentration is 0.0025mol/L, demarcates with titer, and the f that determines this liquid is 1.02.
Take by weighing 0.1000g T.B, be made into the solution of 100.0000g with deionized water, its solute massfraction is 0.1%.
Take by weighing deacetylation and be 90% constant weight chitin fiber m
1(1.0000) 9, it is shredded in the conical flask that is positioned over 150ml, adding solute massfraction is 5% acetum, to m (100.0000) g; Dissolve fully and the complete back of deaeration weighing m
2(1.0000) g solution adds the 50ml deionized water in conical flask, splashes into 3～4 T.B indicator; While stirring the PVSK vs is slowly splashed on magnetic stirring apparatus, solution becomes aubergine by blueness, and colour-fast and flocky precipitate occurs in 30s, is titration end-point, reads the titration volume V of polyvinyl sulfuric acid potassium vs
1Be 21.50ml.
Get the 50ml deionized water, carry out blank titration by above-mentioned same method, and read the titration volume V of polyvinyl sulfuric acid potassium vs
2Be 0.15ml;
Be calculated as follows the deacetylation of chitin fiber:
D·D＝【0.2032×C×f×(V
1-V
2)】/【m
2×(m
1/m)+0.0420×C×f×(V
1-V
2)】×100％
＝【0.2032×0.0025×1.02×(21.50-0.15)】/【1.0000×(1.0000/100.0000)+0.0420×0.0025×1.02×(21.50-0.15)】×100％
＝90.04％
Embodiment four:
Take by weighing 0.4220g PVSK, be made into the solution of 1000ml with deionized water.Concentration is 0.0025mol/L, demarcates with titer, and the f that determines this liquid is 1.04.
Take by weighing 0.1000g T.B, be made into the solution of 100.0000g with deionized water, its solute massfraction is 0.1%.
Take by weighing deacetylation and be 90% constant weight chitin fiber m
1(0.5000) g shreds it in conical flask that is positioned over 100ml, and adding solute massfraction is 1% hydrochloric acid solution, to m (100.0000) g; Dissolve fully and the complete back of deaeration weighing m
2(0.7500) g solution adds the 50ml deionized water in conical flask, splashes into 3～4 T.B indicator; While stirring the PVSK vs is slowly splashed on magnetic stirring apparatus, solution becomes aubergine by blueness, and colour-fast and flocky precipitate occurs in 30s, is titration end-point, reads the titration volume V of polyvinyl sulfuric acid potassium vs
1Be 8.00ml.
Get the 50ml deionized water, carry out blank titration by above-mentioned same method, and read the titration volume V of polyvinyl sulfuric acid potassium vs
2Be 0.15ml;
Be calculated as follows the deacetylation of chitin fiber:
D·D＝【0.2032×C×f×(V
1-V
2)】/【m
2×(m
1/m)+0.0420×C×f×(V
1-V
2)】×100％
＝【0.2032×0.0025×1.04×(8.00-0.15)】/【0.7500×(0.5000/100.0000)+0.0420×0.0025×1.04×(8.00-0.15)】×100％
＝90.02％
Embodiment five:
Take by weighing 0.4220g PVSK, be made into the solution of 1000ml with deionized water.Concentration is 0.0025mol/L, demarcates with titer, and the f that determines this liquid is 0.99.
Take by weighing 0.1000g T.B, be made into the solution of 100.0000g with deionized water, its solute massfraction is 0.1%.
Take by weighing deacetylation and be 90% constant weight chitin fiber m
1(0.7500) g shreds it in conical flask that is positioned over 100ml, and adding solute massfraction is 1% salpeter solution, to m (50.0000) g; Dissolve fully and the complete back of deaeration weighing m
2(0.5000) g solution adds the 50ml deionized water in conical flask, splashes into 3～4 T.B indicator; While stirring the PVSK vs is slowly splashed on magnetic stirring apparatus, solution becomes aubergine by blueness, and colour-fast and flocky precipitate occurs in 30s, is titration end-point, reads the titration volume V of polyvinyl sulfuric acid potassium vs
1Be 16.65ml.
Get the 50ml deionized water, carry out blank titration by above-mentioned same method, and read the titration volume V of polyvinyl sulfuric acid potassium vs
2Be 0.15ml;
Be calculated as follows the deacetylation of chitin fiber:
D·D＝【0.2032×C×f×(V
1-V
2)】/【m
2×(m
1/m)+0.0420×C×f×(V
1-V
2)】×100％
＝【0.2032×0.0025×0.99×(16.65-0.15)】/【0.5000×(0.7500/50.0000)+0.0420×0.0025×0.99×(16.65-0.15)】×100％
＝90.05％。
Claims (2)
1. a method of measuring the chitin fiber deacetylation is characterized in that, may further comprise the steps:
Step 1: the chain link relative molecular mass of the preparation of PVSK solution: PVSK is with C
2H
3OSO
3K is calculated as 162.2040, takes by weighing a certain amount of PVSK, is made into the solution that amount of substance concentration is C mol/L with deionized water; Demarcate with titer again, determine the factor value of this liquid, i.e. correction factor f.
Step 2: the preparation of T.B indicator: take by weighing 0.1000g T.B, be made into the solution of 100.0000g with deionized water, its solute massfraction is 0.1%.
Step 3: taking by weighing constant weight is m
1Gram, chitin fiber to be measured is positioned over it in conical flask, the acid solution that adds the different solute massfractions that are fit to the dissolving chitin fiber, the chitosan solution m that is mixed with different solute massfractions restrains, and is stirred to dissolving fully, after treating bubble collapse, accurate weighing m
2Gram solution adds the 50ml deionized water in conical flask, splash into 3～4 T.B indicator; While stirring the PVSK vs is slowly splashed on magnetic stirring apparatus, solution becomes aubergine by blueness, and colour-fast and flocky precipitate occurs in 30s, is titration end-point, reads the titration volume V of polyvinyl sulfuric acid potassium vs
1Ml.
Step 4: get with step 3 in the deionized water of equal number, carry out blank titration by above-mentioned same method, read the titration volume V of polyvinyl sulfuric acid potassium vs
2Ml;
Step 5: the deacetylation that is calculated as follows chitin fiber:
M
1＝161.1558×C×0.001×f×(V
1-V
2)
M
2＝m
2×(m
1/m)-M
1
D·D＝(M
1/161.1558)/(M
1/161.1558+M
2/203.1925)×100％
With M
1And M
2Substitution:
D·D＝{【161.1558×C×0.001×f×(V
1-V
2)】/161.1558}/{【161.1558×C×0.001×f×(V
1-V
2)】/161.1558+【m
2×(m
1/m)-161.1558×C×0.001×f×(V
1-V
2)】/203.1925}×100％
Calculate at last:
D·D＝【0.2032×C×f×(V
1-V
2)】/【m
2×(m
1/m)+0.0420×C×f×(V
1-V
2)】×100％?。
2. a kind of method of measuring the chitin fiber deacetylation as claimed in claim 1 is characterized in that, in the described step 3, in splashing into polyvinyl sulfuric acid potassium vs process, rate of titration is controlled at 0.03ml/s.
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| PCT/CN2013/085727 WO2014180099A1 (en) | 2013-05-09 | 2013-10-22 | Method for measuring degree of deacetylation of chitosan fibers |
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| CN103983593A (en) * | 2013-11-20 | 2014-08-13 | 中国科学院海洋研究所 | Method used for determining deacetylation degree of mixture of chitosan and chitosan oligosaccharide |
| WO2014180099A1 (en) * | 2013-05-09 | 2014-11-13 | 海斯摩尔生物科技有限公司 | Method for measuring degree of deacetylation of chitosan fibers |
| CN105116112A (en) * | 2015-09-09 | 2015-12-02 | 浙江科技学院 | Method for determining substitution degree of chitosan thiosemicarbazide |
| CN106226304A (en) * | 2016-08-30 | 2016-12-14 | 广东药科大学 | A kind of method utilizing acid-base indicator method to measure oligochitosan deacetylation |
| CN106248607A (en) * | 2016-08-30 | 2016-12-21 | 广东药科大学 | Utilize the method that first derivative ultraviolet spectro-photometry measures oligochitosan deacetylation |
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| JP2004020266A (en) * | 2002-06-13 | 2004-01-22 | Nichiro Corp | Determination method for metering chitosane content in foods |
| CN101368914B (en) * | 2008-10-16 | 2010-09-08 | 杭州天奇印染有限公司 | Method for measuring crust amine and chitin content in crust amine fibre and cotton blending product |
| CN101832938B (en) * | 2010-04-29 | 2012-11-07 | 山东华兴纺织集团有限公司 | Method for measuring content of chitosan fibers contained in textile |
| CN103267757A (en) * | 2013-05-09 | 2013-08-28 | 海斯摩尔生物科技有限公司 | Method for determining deacetylation degree of chitosan fibers |
-
2013
- 2013-05-09 CN CN2013101674878A patent/CN103267757A/en active Pending
- 2013-10-22 WO PCT/CN2013/085727 patent/WO2014180099A1/en active Application Filing
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2014180099A1 (en) * | 2013-05-09 | 2014-11-13 | 海斯摩尔生物科技有限公司 | Method for measuring degree of deacetylation of chitosan fibers |
| CN103983593A (en) * | 2013-11-20 | 2014-08-13 | 中国科学院海洋研究所 | Method used for determining deacetylation degree of mixture of chitosan and chitosan oligosaccharide |
| CN105116112A (en) * | 2015-09-09 | 2015-12-02 | 浙江科技学院 | Method for determining substitution degree of chitosan thiosemicarbazide |
| CN106226304A (en) * | 2016-08-30 | 2016-12-14 | 广东药科大学 | A kind of method utilizing acid-base indicator method to measure oligochitosan deacetylation |
| CN106248607A (en) * | 2016-08-30 | 2016-12-21 | 广东药科大学 | Utilize the method that first derivative ultraviolet spectro-photometry measures oligochitosan deacetylation |
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| Publication number | Publication date |
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| WO2014180099A1 (en) | 2014-11-13 |
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Application publication date: 20130828 |