CN103245781A - Interpretation method of immunochromatographic detection result machine - Google Patents
Interpretation method of immunochromatographic detection result machine Download PDFInfo
- Publication number
- CN103245781A CN103245781A CN2013101516198A CN201310151619A CN103245781A CN 103245781 A CN103245781 A CN 103245781A CN 2013101516198 A CN2013101516198 A CN 2013101516198A CN 201310151619 A CN201310151619 A CN 201310151619A CN 103245781 A CN103245781 A CN 103245781A
- Authority
- CN
- China
- Prior art keywords
- array
- gray
- scale value
- variable
- testing result
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Images
Abstract
The invention relates to an interpretation method of an immunochromatographic detection result machine, belonging to the technical field of immunochromatographic analysis. The interpretation method comprises the following steps of: transforming a region to be identified of a detection test paper image into a gray-level image and storing the gray-level image, wherein the gray-level image comprise n rows of pixels and m lines of pixels, n and m both are non-zero natural numbers; sequentially calculating the sum yi of gray values of m pixels in the ith row in the gray-level image, wherein (i, yi) is used as a gray value array; dividing the n gray value arrays into two parts, wherein one part is an array A and the other part is an array B; fitting a function yA (i) by using yi as a variable and i as an independent variable in the array A, solving a maximum value point in the yA (i), and enabling i=Amax to be used as a control line; fitting a function yB (i) by using yi as a variable and i as an independent variable in the array B, solving a maximum value point and an error (the absolute value of (Bmax-Amax)-L is not more than delta theta) in the yB (i), and enabling i=Bmax to be used as a test line; and judging that whether the test result is negative or positive according to the yBmax. The method provided by the invention is simple in interpretation and high in accuracy and stability.
Description
Technical field
The present invention relates to a kind of immunochromatography testing result machine interpretable method, relate in particular to a kind of judgement and read simply, accurately, and the testing result immunochromatography testing result machine interpretable method that can store and transmit, immunity chromatography test plate detection technique field belonged to.
Background technology
Developing by leaps and bounds of immuno-chromatographic assay technology, constantly the improving of especially golden mark technology, its varieties of reagent is also abundant day by day, and nearly hundreds of at present, the range of application of product comprises viral antigen, antibody test; Hormone detects; Microorganism detection; Tumor markers detects; Medicament residue detects; Drugs detection etc., the immuno-chromatographic assay technology development is very rapid, and the maximum characteristics of this detection method are single part, simple and quick.
But present immunity chromatography test plate detection result mainly relies on naked eyes to come result of determination, and the gray scale of human eye, colourity limiting resolution are limited, is lower than the image of certain resolution, and human eyesight just can't be differentiated.When the concentration of test sample is very low, during almost near critical value, the color development of test colour generation zone colour band or color lump is very shallow, whether human eye is difficult to determine has colour developing to occur on the chromatography test plate (panel), at this moment be easy to cause erroneous judgement, have again, detect the original colour developing result in back each time and all can't keep file, so practicality has been subjected to very big restriction.
Also there are some quantitative pick-up units to occur at present, but the sensitivity of accepting sensor itself owing to light is limited, add the noise reason of components and parts, when the concentration that is detected sample is very low, during almost near critical value, the color development of test colour generation zone colour band or color lump is very shallow, and this quasi-instrument also is to tell whether color is arranged, and occurs wrong result of determination easily.
No matter directly with the naked eye differentiate immunity chromatography test plate detection result, still the device of making by photoelectric sensor is differentiated testing result, all exist the limited problem of the resolution of gray scale, colourity, and easily because of the sensitivity of tester's individual difference XOR device, the judgement conclusion that must make mistake.
In sum, the shortcoming that prior art exists is: by the device that artificial naked eyes or photoelectric sensor are made immunity chromatography test plate detection result is differentiated, accuracy and the poor stability differentiated can not carry out quantitative test, and can't preserve original image document.
Summary of the invention
For overcoming the shortcoming that exists in the prior art, goal of the invention of the present invention provides a kind of immunochromatography testing result machine interpretable method, and method judgement provided by the invention is read simple, accurate.
For realizing described goal of the invention, the invention provides a kind of immunochromatography testing result machine interpretable method, it comprises the steps:
The first step: will detect test paper image zone to be identified and change into grayscale image and preserve, described grayscale image by n capable and m row pixel form, wherein, n and m are the non-zero natural number;
Second step: the gray-scale value sum y that calculates m the pixel in capable of i in the grayscale image successively
i, (i, y
i) as a gray-scale value array, i=1 wherein, 2 ..., n;
The 3rd step: n gray-scale value array is divided into two parts, and a copy of it is array A, and another part is array B;
The 4th the step: in array A with y
iBeing variable, is independent variable with i, fitting function y
A(i), obtain y
A(i) extreme point in
If
Be maximum of points, then i=A
MaxBe control line;
The 5th the step: in array B with y
iBeing variable, is independent variable with i, fitting function y
B(i), obtain y
B(i) extreme point in
If
For maximum of points and (| B
Max-A
Max)-L|≤Δ δ, then i=B
MaxBe p-wire, wherein, L is the fixed range between test paper p-wire and the control line, and Δ δ is error;
The 6th step: according to
Size judge that test result is negative or positive.
Preferably, it is characterized in that described n is even number.
Preferably, it is characterized in that described array A is by
Individual gray-scale value array is formed, and array B is by the
Individual gray-scale value array is formed;
Preferably, described n is odd number.
Preferably, described array A is by
Individual gray-scale value array is formed, and array B is by the
Individual gray-scale value array is formed;
Preferably, described array A is by
Individual gray-scale value array is formed, and array B is by the
Individual gray-scale value array is formed.
Compare with present technology, method interpretation provided by the invention is simple, and accuracy and stability are high, can carry out quantitative test, and the result can preserve.
Description of drawings
Fig. 1 is the block diagram of immunochromatography detector provided by the invention;
Fig. 2 is test paper grayscale image synoptic diagram provided by the invention;
Fig. 3 is the curve map that the invention provides the grey scale pixel value array.
Embodiment
Describe principle of work of the present invention in detail below in conjunction with accompanying drawing.
Fig. 1 is the block diagram of immunochromatography detector provided by the invention.As shown in Figure 1, described immunochromatography detector comprises that optical lens, photoelectric commutator, analogue signal processor, A/D converter, digital signal processor, image controller, CPU, shutter aperture driving circuit, focusing zoom drive driving circuit, illuminating lamp, communication module, Big Dipper locating module, storer, touch-screen, LCD and interface circuit.Wherein optical lens receives the reflected light of colloidal gold test plate and focuses on the photoelectric commutator, photoelectric commutator converts light image to electrical picture signal, analogue signal processor is used for amplifying electrical picture signal, A/D converter converts electrical picture signal to data image signal, digital signal processor word picture signal is encoded and is become the picture signal of jpeg format with compressing and converting and send CPU to, shutter aperture driving circuit is controlled shutter and the aperture of optical lens under the control of CPU, the focusing zoom drives driving circuit and make optical lens focusing and zoom under the control of CPU, and illuminating lamp is used for providing light source to the colloidal gold test plate under the control of CPU, touch-screen is used for the input instruction; LCD is used for showing the geographic position data at test pattern and test result and tester place; Locating module can be the GPS locating module, also can be Big Dipper locating module, is used for providing the geographic position data of tester under the control of CPU, and storer is used for storage data, operating system and application program; Interface circuit can connect keyboard, can also connect display, printer etc., is controlled by CPU; Communication module can be given long-range host computer with test pattern, result and tester geographic location data transmission under the control of CPU.
After digital signal processor was input to CPU with the data image signal of colloidal gold test plate, CPU handled this picture signal as follows:
The first step: will detect test paper image zone to be identified and change into grayscale image and preserve, described grayscale image by n capable and m row pixel form, wherein, n and m are the non-zero natural number, as shown in Figure 2;
Second step: the gray-scale value sum y that calculates m the pixel in capable of i in the grayscale image successively
i, (i, y
i) as a gray-scale value array, i=1 wherein, 2 ..., n;
The 3rd step: n gray-scale value array is divided into two parts, and a copy of it is array A, and another part is array B; When n was even number, described array A was by
Individual gray-scale value array is formed, and array B is by the
Individual gray-scale value array is formed;
When n was odd number, described array A was by
Individual gray-scale value array is formed, and array B is by the
Individual gray-scale value array is formed; Perhaps described array A is by
Individual gray-scale value array is formed, and array B is by the
Individual gray-scale value array is formed.
The 4th the step: in array A with y
iBeing variable, is independent variable with i, fitting function y
A(i), obtain y
A(i) extreme point in
If
Be maximum of points, then i=A
MaxBe control line;
The 5th the step: in array B with y
iBeing variable, is independent variable with i, fitting function y
B(i), obtain y
B(i) extreme point in
If
For maximum of points and (| B
Max-A
Max)-L|≤Δ δ, then i=B
MaxBe p-wire, wherein, L is the fixed range between test paper p-wire and the control line, and Δ δ is error;
The 6th step: according to
Size judge that test result is negative or positive.The actual inspection value belongs to negative or positive, depends on concrete reagent.Some reagent is higher than the positive of calibration value; Some reagent is opposite.
That is to say, said process can be written as should program to be stored in the storer, when testing, call this application program and get final product.
More than be described with reference to the accompanying drawings the present invention, but instructions is only for explaining claims.But protection scope of the present invention is not limited to instructions.Anyly be familiar with those skilled in the art in the technical scope of this disclosure, the variation that can expect easily or replacement all should be encompassed within protection scope of the present invention.Therefore, protection scope of the present invention should be as the criterion with the protection domain of claims.
Claims (6)
1. immunochromatography testing result machine interpretable method, it comprises the steps:
The first step: will detect test paper image zone to be identified and change into grayscale image and preserve, described grayscale image by n capable and m row pixel form, wherein, n and m are the non-zero natural number;
Second step: the gray-scale value sum y that calculates m the pixel in capable of i in the grayscale image successively
i, (i, y
i) as a gray-scale value array, i=1 wherein, 2 ..., n;
The 3rd step: n gray-scale value array is divided into two parts, and a copy of it is array A, and another part is array B;
The 4th the step: in array A with y
iBeing variable, is independent variable with i, fitting function y
A(i), obtain y
A(i) extreme point in
If
Be maximum of points, then i=A
MaxBe control line;
The 5th the step: in array B with y
iBeing variable, is independent variable with i, fitting function y
B(i), obtain y
B(i) extreme point in
If
For maximum of points and (| B
Max-A
Max)-L|≤Δ δ, then i=B
MaxBe p-wire, wherein, L is the fixed range between test paper p-wire and the control line, and Δ δ is error;
The 6th step: according to
Size judge that test result is negative or positive.
2. immunochromatography testing result machine interpretable method according to claim 1 is characterized in that described n is even number.
3. immunochromatography testing result machine interpretable method according to claim 2 is characterized in that, described array A is by
Individual gray-scale value array is formed, and array B is by the
Individual gray-scale value array is formed;
4. immunochromatography testing result machine interpretable method according to claim 1 is characterized in that described n is odd number.
5. immunochromatography testing result machine interpretable method according to claim 4 is characterized in that, described array A is by
Individual gray-scale value array is formed, and array B is by the
Individual gray-scale value array is formed;
6. immunochromatography machine interpretable method according to claim 4 is characterized in that, described array A is by
Individual gray-scale value array is formed, and array B is by the
Individual gray-scale value array is formed.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310151619.8A CN103245781B (en) | 2013-04-27 | 2013-04-27 | Interpretation method of immunochromatographic detection result machine |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310151619.8A CN103245781B (en) | 2013-04-27 | 2013-04-27 | Interpretation method of immunochromatographic detection result machine |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN103245781A true CN103245781A (en) | 2013-08-14 |
| CN103245781B CN103245781B (en) | 2015-05-27 |
Family
ID=48925430
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201310151619.8A Active CN103245781B (en) | 2013-04-27 | 2013-04-27 | Interpretation method of immunochromatographic detection result machine |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN103245781B (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103616522A (en) * | 2013-10-28 | 2014-03-05 | 中国科学院苏州生物医学工程技术研究所 | Immune chromatography result identification method based on envelope area and twice compensation |
| CN105606608A (en) * | 2016-03-28 | 2016-05-25 | 广州博厚生物科技有限公司 | Image gray-scale processing based data computing method and application thereof in detection field |
| CN106056156A (en) * | 2016-05-31 | 2016-10-26 | 武汉明德生物科技股份有限公司 | System and method of recognizing and detecting reagent card |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US7197169B2 (en) * | 2003-01-02 | 2007-03-27 | Kuo-Jeng Wang | Method for detecting a response of each probe zone on a test strip |
| CN102384972A (en) * | 2010-10-19 | 2012-03-21 | 刘江 | Machine vision-based immunochromatography result interpretation method |
| CN102507565A (en) * | 2011-11-30 | 2012-06-20 | 刘江 | Method for searching reaction color development area of immunochromatographic strip |
| CN102692498A (en) * | 2012-06-11 | 2012-09-26 | 华中农业大学 | Automatic code reading device and automatic code reading method of sulfanilamide type medicine residue detecting reagent strip |
-
2013
- 2013-04-27 CN CN201310151619.8A patent/CN103245781B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US7197169B2 (en) * | 2003-01-02 | 2007-03-27 | Kuo-Jeng Wang | Method for detecting a response of each probe zone on a test strip |
| CN102384972A (en) * | 2010-10-19 | 2012-03-21 | 刘江 | Machine vision-based immunochromatography result interpretation method |
| CN102507565A (en) * | 2011-11-30 | 2012-06-20 | 刘江 | Method for searching reaction color development area of immunochromatographic strip |
| CN102692498A (en) * | 2012-06-11 | 2012-09-26 | 华中农业大学 | Automatic code reading device and automatic code reading method of sulfanilamide type medicine residue detecting reagent strip |
Non-Patent Citations (4)
| Title |
|---|
| 刘蕾等: "基于一维CCD的免疫层析试纸条检测系统", 《仪器仪表学报》, vol. 28, no. 02, 28 February 2007 (2007-02-28) * |
| 康健: "基于ARM的农产品质量快速检测仪的研究", 《万方数据企业知识服务平台》, 25 December 2012 (2012-12-25) * |
| 杨成文: "基于机器视觉胶体金试纸条显色分析仪", 《中国优秀硕士学位论文全文数据库工程科技Ⅱ辑》, no. 2, 16 November 2011 (2011-11-16) * |
| 赵友全等: "一种新型便携式甲霜灵胶体金试纸条显色分析仪的研制", 《仪器仪表学报》, vol. 30, no. 10, 15 October 2009 (2009-10-15), pages 2175 - 2179 * |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103616522A (en) * | 2013-10-28 | 2014-03-05 | 中国科学院苏州生物医学工程技术研究所 | Immune chromatography result identification method based on envelope area and twice compensation |
| CN105606608A (en) * | 2016-03-28 | 2016-05-25 | 广州博厚生物科技有限公司 | Image gray-scale processing based data computing method and application thereof in detection field |
| CN105606608B (en) * | 2016-03-28 | 2018-12-07 | 广州博厚医疗技术有限公司 | Based on image grayscale processing method for computing data and its in the application of detection field |
| CN106056156A (en) * | 2016-05-31 | 2016-10-26 | 武汉明德生物科技股份有限公司 | System and method of recognizing and detecting reagent card |
Also Published As
| Publication number | Publication date |
|---|---|
| CN103245781B (en) | 2015-05-27 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN100458670C (en) | Location detecting apparatus using area image sensor | |
| RU2571411C2 (en) | Method and device for identification of erroneous image data representation on display unit | |
| CN101685595B (en) | System and method for correcting gamma | |
| CN101251496A (en) | Defect detection device and defect detection method | |
| CN106839976B (en) | Method and device for detecting lens center | |
| CN107886920B (en) | Method and system for obtaining correct Mura compensation data | |
| CN103868925B (en) | Detection device and detection method | |
| CN103559484B (en) | The method for quickly identifying of measuring instrument graduation mark | |
| CN103245780A (en) | Colloidal gold anti-counterfeiting tracing cloud platform | |
| JP2008175549A (en) | Defect detection device and method | |
| CN103245781B (en) | Interpretation method of immunochromatographic detection result machine | |
| US11917125B2 (en) | Test system and test method for stability of video interface | |
| CN103245782B (en) | Colloidal gold method on-line detector | |
| CN102565062B (en) | Method for testing turbidity of liquid based on detection of image gray | |
| KR20140075042A (en) | Apparatus for inspecting of display panel and method thereof | |
| CN100570342C (en) | Method of optically measuring and device thereof | |
| CN102841102B (en) | Recognition method and device for micro-scale damage point in damage threshold measurement | |
| CN101140307B (en) | Automatically visual inspection method | |
| JP6803940B2 (en) | Remote meter reading computer, its method and program | |
| CN102387631A (en) | Automatic judgment and adjustment method of light intensity in immunochromatography interpretation recorder | |
| US20100303306A1 (en) | Method and system for measuring text-rendering quality | |
| CN1821715A (en) | Novel hair online measuring integrated device | |
| CN101532819A (en) | Method for detecting printed circuit board with X-rays | |
| JP2008032653A (en) | Test pattern generating method, data structure of test pattern, test pattern generating device, display panel inspection system, control program, and computer readable recording medium with the program recorded thereon | |
| CN113390869A (en) | Test strip detection device |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CP03 | Change of name, title or address | ||
| CP03 | Change of name, title or address |
Address after: 100043 Beijing City, Shijingshan District City West Road No. 33 A block 4 layer Patentee after: Beijing Fu Yun Cloud Data Technology Co., Ltd. Address before: 100041 Beijing, Badachu hi tech park, West Wells Road, building 1161, room 3, No., room 3 Patentee before: Beijing Fule Cloud Inspection Technology Co., Ltd. |