CN103245652A - Method for detecting lead ions by forming G-quadruplex quenching fluorescent light by using functional nucleic acid - Google Patents
Method for detecting lead ions by forming G-quadruplex quenching fluorescent light by using functional nucleic acid Download PDFInfo
- Publication number
- CN103245652A CN103245652A CN2013101807808A CN201310180780A CN103245652A CN 103245652 A CN103245652 A CN 103245652A CN 2013101807808 A CN2013101807808 A CN 2013101807808A CN 201310180780 A CN201310180780 A CN 201310180780A CN 103245652 A CN103245652 A CN 103245652A
- Authority
- CN
- China
- Prior art keywords
- nucleic acid
- functional nucleic
- fluorescence
- lead
- lead ion
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Images
Abstract
The invention relates to a method for detecting lead ions by forming G-quadruplex quenching fluorescent light by using functional nucleic acid, belonging to the technical field of water quality detection. The method comprises the following steps of: marking the functional nucleic acid by using carboxy fluorescein to construct a lead ion detection system, wherein when lead ions are added into the detection system, the lead ions promote the functional nucleic acid to form a G-quadruplex structure to cause fluorescence signals of the whole system to significantly change around 520nm, and the change of the fluorescence signals is in a positive relationship with the concentration of the lead ions; then carrying out fluorescence spectrum scanning by adopting a fluorescent photometer to obtain a fluorescence quenching rate standard curve; and mixing a water sample to be tested and ultrapure water, namely contrast samples, with a mother solution of the functional nucleic acid respectively, and comparing the fluorescence quenching rates of two mixed products at a part of which the wavelength is 480nm with the fluorescent quenching rate standard curve to obtain the concentration of the lead ions in the water sample to be tested. By adopting the method, the defects that a lot of assistants are needed to be introduced during the detection of the lead ions by using the existing functional nucleic acid, a complex catalytic reaction is related and the like are overcome, and efficient water body lead ion detection of which the lowest detection limit is 0.77ppb and the cost is low is realized by adopting simple operation.
Description
Technical field
What the present invention relates to is the method in the plumbous detection technique of a kind of water body field, specifically is a kind of method of utilizing functional nucleic acid to form G-tetrad cancellation fluoroscopic examination lead ion.
Background technology
Lead ion is as a kind of modal heavy metal contaminants, its high toxicity and the harm that environment, the mankind cause has been caused the extensive concern in the whole world.Lead ion in the environment has persistence, easily animal migration and biological concentration highly.Enter the lead of human body, 90% is stored in bone, 10% is distributed to each organ of whole body and tissue with blood, influence brain, kidney, nervous system and erythrocyte function etc., after particularly the infant absorbs lead, will have to surpass 30% reservation in vivo, influence infant's growth and intelligence development, and damaging brain functions such as its cognitive function, neurobehavioral and learning and memory, severe patient causes dementia.Therefore, the lead ion detection method of setting up high sensitivity, high selectivity seems particularly important.
Traditional lead ion detection method has atomic absorption spectrography (AAS), atomic emission spectrometry, inductively coupled plasma mass spectrometry, atomic fluorescence spectrometry and vapor-phase chromatography, though these methods accurately and reliably, but need expensive complicated instrument and equipment and professional and technical personnel in actual applications, it is time-consuming to require great effort, and is difficult to satisfy the needs of large-scale application and real-time in-situ detection.Novel lead ion detection technique has chemical sensor technology and biosensor technology, and wherein functional nucleic acid class biology sensor characteristics such as selectivity is good because having, the signal conversion is easy gradually become focus.Functional nucleic acid for detection of lead generally includes two classes: a class is nuclease (DNAzyme), this fermentoid detects in the substrate chain that plumbous principle is enzyme and comprises a ribose adenine (rA), lead ion can cut off from this position with the substrate chain of enzyme specifically, reaches testing goal by the substrate chain break being changed into signals such as colorimetric, chemiluminescence and fluorescence; Another kind of is the single stranded oligonucleotide that is rich in guanine (G), lead ion can promote this class oligonucleotide to form G-tetrad (G-quadruplex) structure, correlation properties in conjunction with the G-tetrad are selected the appropriate signal output intent, also can reach to detect plumbous purpose.Compare with nuclease, it is low that oligonucleotides has a synthetic cost, characteristics such as good stability, thereby become the research focus day by day.
Fluorescence method is a kind of easy, quick, sensitive detection method, be used to detect multiple material in conjunction with functional nucleic acid (especially single stranded oligonucleotide), detection about lead ion also has report, but these methods maybe need to introduce more adminicle, or relates to complicated catalytic reaction.And the method for utilizing functional nucleic acid to form G-tetrad cancellation fluoroscopic examination lead ion is still rarely reported.
Find through the retrieval to prior art, Chinese patent literature CN102703441A, open day 2012-10-03 discloses a kind of hair clip type nucleic acid fluorescent probe, and the sequence of this hair clip type nucleic acid fluorescent probe is 5 '-FAM-CGTCATGGGTGGGTGGGTGGGTGACGGGG-3 '.The purposes of hair clip type nucleic acid fluorescent probe in detecting lead ion, using method is made up of preparation hair clip type nucleic acid probe storing solution, preparation hair clip type nucleic acid probe storing solution, preparation testing sample solution, working sample step, by the variation of detection architecture fluorescence intensity, can realize the detection of lead ion.But the weak point of this technology is that its detection to lead ion is based on the transformation of nucleic acid structure from hair clip type to G-tetrad configuration, and the hair clip type structure of this nucleic acid is to keep by 5 complementary bases formation base pairings at sequence two ends.Base forms the stand-by period (15min) that pairing is difficult to guarantee and needs are grown at ambient temperature, and these can influence stability and the agility of probe.
Summary of the invention
The present invention is directed to the prior art above shortcomings, a kind of method of utilizing functional nucleic acid to form G-tetrad cancellation fluoroscopic examination lead ion is proposed, overcome existing capability detection of nucleic acids lead ion and need introduce numerous adminiclies, relate to complicated defectives such as catalytic reaction, adopted simple operations to realize that lowest detection is limited to 0.77ppb, low cost and water body lead ion detection efficiently.
The present invention is achieved by the following technical solutions, the present invention is by using Fluoresceincarboxylic acid (FAM) mark function nucleic acid, make up the lead ion detection architecture, after adding lead ion in the detection architecture, lead ion promotes functional nucleic acid to form G-tetrad structure, marked change takes place in the fluorescence signal that causes whole system about 520nm, and fluorescence signal changes and plumbum ion concentration presents positive relationship, adopts fluorospectrophotometer to carry out fluorescence spectrum scanning then and obtains fluorescent quenching rate typical curve; During test with water sample to be measured and ultrapure water, namely to after mixing with Fluoresceincarboxylic acid mark function nucleic acid mother liquor respectively in the same old way, measure two mix products in the fluorescence intensity at 480nm wavelength place, calculate the cancellation rate, contrast fluorescent quenching rate typical curve obtains the plumbum ion concentration in the water sample to be measured.
Described Fluoresceincarboxylic acid mark function nucleic acid refers to: sequence is shown in Seq ID No.1, that is: the nucleic acid of 5'-GGGTGGGTGGGTGGGT-3', and 5' end is modified through FAM.
Described quartzy fluorescence cuvette is: internal diameter is 0.5cm * 0.5cm, and external diameter is 1.0cm * 1.0cm.
Described fluorescent quenching rate typical curve refers to: adopt fluorospectrophotometer to carry out the fluorescence intensity that fluorescence spectrum scanning obtains the 480nm place, calculate the cancellation rate, with the fluorescent quenching rate of the standard solution of variable concentrations and blank system solution as ordinate, the regression equation that the concentration of lead ion obtains as horizontal ordinate in the mixed liquor, i.e. typical curve.
Described scanning more preferably adopts quartzy fluorescence to hold detection architecture with cuvette, and the exciting light slit is 10nm, and the emission optical slits is 10nm, and excitation wavelength is to scan under the 480nm condition.
Described detection architecture prepares by following steps:
1) in the 2mL graduated centrifuge tube, adding 2.5 μ L concentration is the Fluoresceincarboxylic acid mark function nucleic acid mother liquor of 5 μ M, adds deionized water to 450 μ L, fully centrifuge tube is placed under 25 ° of C conditions standby behind the mixing again.
2) get 16 and comprise set by step 1) the method preparation form the centrifuge tube of detection architecture mixed liquor by functional nucleic acid, add 50 μ L variable concentrations lead ion titers respectively, make the lead ion content in the whole detection architecture maintain 0.5 – 200ppb, fully centrifuge tube is placed under 25 ° of C conditions again behind the mixing and hatch 5min;
3) get 1 in addition and comprise set by step 1) the method preparation forms detection architecture mixed liquor centrifuge tube by functional nucleic acid, add 50 μ L ultrapure waters, set by step 2) method handles afterwards as the blank system solution;
4) get 500 μ L steps 2 respectively) and standard solution and the blank liquid of step 3) preparation, place quartzy fluorescence with cuvette, carry out the sweep measuring signal with fluorospectrophotometer, the fluorescence spectrum signal that obtains lead ion and blank liquid is respectively F and F
0, calculate fluorescent quenching rate Q=[F
0-F]/F
0, and with lead at different concentrations (C
Pb) with corresponding fluorescent quenching rate Q mapping, drawing standard curve.
The actual conditions of described scanning is: the exciting light slit is 10nm, and the emission optical slits is 10nm, and excitation wavelength is to scan under the 480nm condition, obtains its fluorescence signal spectrum.
Principle of the present invention is: when having only functional nucleic acid in the detection architecture, the fluorescence signal of system about 520nm is very strong, when adding lead ion in the detection architecture, functional nucleic acid can with the lead ion effect, form the G-tetrad, cause that marked change takes place at the 520nm place fluorescence signal of whole system, and fluorescence signal changes and lead concentration presents positive relationship, so change by the analysis of fluorescence signal, just can realize that lead ion detects in the water body.
Technique effect
Compared with prior art, the present invention no longer relies on nucleic acid formation hairpin structure to the detection of lead, has removed the complementary base at nucleotide sequence two ends, has shortened sequence length, and this can strengthen the stability of detection to a certain extent and reduce the synthetic cost of nucleic acid.In the present invention simultaneously, lead ion contacts with nucleic acid, can cause the fluorescent quenching effect fast, and the reaction time is no more than 5min, has guaranteed the agility that detects.And detection method provided by the invention does not need large-scale instrument and equipment, the detection sensitivity height, and selectivity is good, and is simple to operate, and whether the lead ion content that can be used for detecting in the potable water exceeds standard.
Description of drawings
Fig. 1 forms G-tetrad cancellation fluoroscopic examination lead ion synoptic diagram for functional nucleic acid.
Fig. 2 adds fluorescence signal after the detection architecture that functional nucleic acid forms for the variable concentrations lead ion.
Fig. 3 is the relation of fluorescent quenching rate and variable concentrations lead ion.
Fig. 4 adds fluorescent quenching rate after the detection architecture that functional nucleic acid forms for the different metal ion.
Embodiment
Below embodiments of the invention are elaborated, present embodiment is being to implement under the prerequisite with the technical solution of the present invention, provided detailed embodiment and concrete operating process, but protection scope of the present invention is not limited to following embodiment.
Embodiment 1
Present embodiment may further comprise the steps:
1) prepares the detection architecture that is formed by functional nucleic acid: in the 2mL graduated centrifuge tube, adding 2.5 μ L concentration respectively is 5 μ M functional nucleic acids (sequence is 5'-GGGTGGGTGGGTGGGT-3') mother liquors, add deionized water to 450 μ L, fully centrifuge tube is placed under 25 ° of C conditions standby behind the mixing again.
2) detection architecture of the known plumbum ion concentration of preparation: get 16 and comprise set by step 1) the method preparation forms the centrifuge tube of detection architecture mixed liquor by functional nucleic acid, add 50 μ L variable concentrations lead ion titers respectively, make the lead content in the whole detection architecture maintain 0.5 – 200ppb, fully centrifuge tube is placed under 25 ° of C conditions again behind the mixing and hatch 5min, give over to following mensuration and use.
3) get 1 in addition and comprise set by step 1) the method preparation forms detection architecture mixed liquor centrifuge tube by functional nucleic acid, add 50 μ L ultrapure waters, set by step 2) method handles afterwards as the blank system solution.
4) get 500 μ L steps 2 respectively) and standard solution and the blank liquid of step 3) preparation, (internal diameter is 0.5cm * 0.5cm, and external diameter is among the 1.0cm * 1.0cm), to carry out the sweep measuring signal with fluorospectrophotometer with cuvette to place quartzy fluorescence.The concrete condition of scanning is: the exciting light slit is 10nm, and the emission optical slits is 10nm, and excitation wavelength is to scan under the 480nm condition, obtains its fluorescence signal spectrum.The fluorescence spectrum signal of lead ion and blank liquid is respectively F and F
0, calculate fluorescent quenching rate Q=[F
0-F]/F
0
5) with lead at different concentrations (C
Pb) with corresponding fluorescent quenching rate Q mapping, drawing standard curve, its regression equation are Q=2.23C
Pb+ 16.05.
6) preparation sample detection system: get 50 μ L water sample to be measured, join set by step 1) being formed in the detection architecture mixed liquor centrifuge tube by functional nucleic acid of method preparation, fully centrifuge tube being placed under 25 ° of C conditions again behind the mixing and hatches 5min, set by step 4) method measures its Q.
7) Q that records per sample looks into typical curve, can be in the hope of lead ion content in the sample.
8) checking: measure 3 parts with the inventive method and contain each portion of polyion mixed liquor that plumbum ion concentration is respectively 15ppb, 50ppb and 100ppb, the recovery that obtains is 95.3%-110.8%, has proved the reliability of this method.
9) concentration range of this method mensuration water body lead ion is 0.5 – 200ppb, and lowest detection is limited to 0.77ppb.
Claims (7)
1. method of utilizing functional nucleic acid to form G-tetrad cancellation fluoroscopic examination lead ion, it is characterized in that, by using Fluoresceincarboxylic acid mark function nucleic acid, make up the lead ion detection architecture, after adding lead ion in the detection architecture, lead ion promotes functional nucleic acid to form G-tetrad structure, adopts fluorospectrophotometer to carry out fluorescence spectrum scanning then and obtains fluorescent quenching rate typical curve; During test with water sample to be measured and ultrapure water, namely to after mixing with Fluoresceincarboxylic acid mark function nucleic acid mother liquor respectively in the same old way, the fluorescent quenching rate contrast fluorescent quenching rate typical curve of two mix products at 480nm wavelength place obtained plumbum ion concentration in the water sample to be measured.
2. method according to claim 1, it is characterized in that, described fluorescent quenching rate typical curve refers to: adopt fluorospectrophotometer to carry out the fluorescence intensity that fluorescence spectrum scanning obtains the 480nm place, calculate the fluorescent quenching rate, with the fluorescent quenching rate of the standard solution of variable concentrations and blank system solution as ordinate, the regression equation that plumbum ion concentration in the mixed liquor obtains as horizontal ordinate, i.e. typical curve.
3. method according to claim 2 is characterized in that, described scanning adopts quartzy fluorescence to hold detection architecture with cuvette, and the exciting light slit is 10nm, and the emission optical slits is 10nm, and excitation wavelength is to scan under the 480nm condition.
4. method according to claim 1 is characterized in that, described detection architecture prepares by following steps:
1) in the 2mL graduated centrifuge tube, adding 2.5 μ L concentration is the Fluoresceincarboxylic acid mark function nucleic acid mother liquor of 5 μ M, adds deionized water to 450 μ L, fully centrifuge tube is placed under 25 ° of C conditions standby behind the mixing again;
2) get 16 and comprise set by step 1) the method preparation form the centrifuge tube of detection architecture mixed liquor by functional nucleic acid, add 50 μ L variable concentrations lead ion titers respectively, make the lead ion content in the whole detection architecture maintain 0.5 – 200ppb, fully centrifuge tube is placed under 25 ° of C conditions again behind the mixing and hatch 5min;
3) get 1 in addition and comprise set by step 1) the method preparation forms detection architecture mixed liquor centrifuge tube by functional nucleic acid, add 50 μ L ultrapure waters, set by step 2) method handles afterwards as the blank system solution;
4) get 500 μ L steps 2 respectively) and standard solution and the blank liquid of step 3) preparation, place quartzy fluorescence with cuvette, carry out the sweep measuring signal with fluorospectrophotometer, the fluorescence spectrum signal that obtains lead ion and blank liquid is respectively F and F
0, calculate fluorescent quenching rate Q=[F
0-F]/F
0, and with lead at different concentrations C
PbWith corresponding fluorescent quenching rate Q mapping, drawing standard curve.
5. method according to claim 4 is characterized in that, the sequence of described functional nucleic acid shown in Seq ID No.1, that is: 5'-GGGTGGGTGGGTGGGT-3'.
6. method according to claim 4 is characterized in that, the actual conditions of described scanning is: the exciting light slit is 10nm, and the emission optical slits is 10nm, and excitation wavelength is to scan under the 480nm condition, obtains its fluorescence signal spectrum.
7. according to claim 1 or 2 or 4 described methods, it is characterized in that described typical curve is: Q=2.23C
Pb+ 16.05, wherein: Q is the fluorescent quenching rate, C
PbBe lead concentration.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2013101807808A CN103245652A (en) | 2013-05-16 | 2013-05-16 | Method for detecting lead ions by forming G-quadruplex quenching fluorescent light by using functional nucleic acid |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2013101807808A CN103245652A (en) | 2013-05-16 | 2013-05-16 | Method for detecting lead ions by forming G-quadruplex quenching fluorescent light by using functional nucleic acid |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN103245652A true CN103245652A (en) | 2013-08-14 |
Family
ID=48925312
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2013101807808A Pending CN103245652A (en) | 2013-05-16 | 2013-05-16 | Method for detecting lead ions by forming G-quadruplex quenching fluorescent light by using functional nucleic acid |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN103245652A (en) |
Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103616365A (en) * | 2013-12-11 | 2014-03-05 | 山东省科学院海洋仪器仪表研究所 | Detection device and method for measuring lead in water by flow injection fluorescence quenching method |
| CN103792229A (en) * | 2014-01-16 | 2014-05-14 | 中国科学院化学研究所 | Detection method for lead ion concentration and kit |
| CN105259314A (en) * | 2015-10-26 | 2016-01-20 | 广东省生态环境与土壤研究所 | Lead ion visual detection method and detection kit |
| CN108918489A (en) * | 2018-07-10 | 2018-11-30 | 同济大学 | A kind of lead ion fluorescence detection method and the nano-particle fluorescence sensor for lead ion detection |
| CN110471098A (en) * | 2019-08-27 | 2019-11-19 | 广东医科大学 | Radon dose of radiation detection method based on carbon quantum dot biological sensor |
| US10677790B2 (en) | 2016-07-20 | 2020-06-09 | City University Of Hong Kong | Optochemical detector and a method for fabricating an optochemical detector |
| CN111487227A (en) * | 2020-01-17 | 2020-08-04 | 商丘师范学院 | Be used for detecting Pb in human serum2+Fluorescence-enhanced sensor of concentration |
| CN113125399A (en) * | 2021-04-14 | 2021-07-16 | 四川大学 | Lead ion detection method based on G-quadruplex proportion fluorescence |
| CN113252623A (en) * | 2021-04-08 | 2021-08-13 | 陕西省石油化工研究设计院 | Homogeneous phase detection method for lead ions based on two-dimensional MOF fluorescence resonance energy transfer |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2002000006A2 (en) * | 2000-06-27 | 2002-01-03 | Board Of Trustees Of The University Of Illinois | Nucleic acid enzyme biosensor for ions |
| CN102586429A (en) * | 2012-01-20 | 2012-07-18 | 上海出入境检验检疫局机电产品检测技术中心 | Lead ion fluorescent DNA (Deoxyribose Nucleic Acid) probe and fluorescent determination method for lead ion concentration |
| CN102703441A (en) * | 2012-04-28 | 2012-10-03 | 陕西师范大学 | Hairpin type nucleic acid fluorescent probe and application of probe in lead ion detection |
-
2013
- 2013-05-16 CN CN2013101807808A patent/CN103245652A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2002000006A2 (en) * | 2000-06-27 | 2002-01-03 | Board Of Trustees Of The University Of Illinois | Nucleic acid enzyme biosensor for ions |
| CN102586429A (en) * | 2012-01-20 | 2012-07-18 | 上海出入境检验检疫局机电产品检测技术中心 | Lead ion fluorescent DNA (Deoxyribose Nucleic Acid) probe and fluorescent determination method for lead ion concentration |
| CN102703441A (en) * | 2012-04-28 | 2012-10-03 | 陕西师范大学 | Hairpin type nucleic acid fluorescent probe and application of probe in lead ion detection |
Non-Patent Citations (1)
| Title |
|---|
| FENG LI,ET AL.: "Crystal violet as a G-quadruplex-selective probe for sensitive amperometric sensing of lead", 《CHEM. COMM.》, vol. 47, 21 November 2011 (2011-11-21), pages 11909 - 11911 * |
Cited By (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103616365A (en) * | 2013-12-11 | 2014-03-05 | 山东省科学院海洋仪器仪表研究所 | Detection device and method for measuring lead in water by flow injection fluorescence quenching method |
| CN103616365B (en) * | 2013-12-11 | 2016-03-30 | 山东省科学院海洋仪器仪表研究所 | Flow injection Fluorimetric Quenching Method measures pick-up unit plumbous in water and method |
| CN103792229A (en) * | 2014-01-16 | 2014-05-14 | 中国科学院化学研究所 | Detection method for lead ion concentration and kit |
| CN103792229B (en) * | 2014-01-16 | 2016-01-27 | 中国科学院化学研究所 | A kind of plumbum ion concentration detection method and kit |
| CN105259314A (en) * | 2015-10-26 | 2016-01-20 | 广东省生态环境与土壤研究所 | Lead ion visual detection method and detection kit |
| US10677790B2 (en) | 2016-07-20 | 2020-06-09 | City University Of Hong Kong | Optochemical detector and a method for fabricating an optochemical detector |
| CN108918489A (en) * | 2018-07-10 | 2018-11-30 | 同济大学 | A kind of lead ion fluorescence detection method and the nano-particle fluorescence sensor for lead ion detection |
| CN110471098A (en) * | 2019-08-27 | 2019-11-19 | 广东医科大学 | Radon dose of radiation detection method based on carbon quantum dot biological sensor |
| CN111487227A (en) * | 2020-01-17 | 2020-08-04 | 商丘师范学院 | Be used for detecting Pb in human serum2+Fluorescence-enhanced sensor of concentration |
| CN113252623A (en) * | 2021-04-08 | 2021-08-13 | 陕西省石油化工研究设计院 | Homogeneous phase detection method for lead ions based on two-dimensional MOF fluorescence resonance energy transfer |
| CN113125399A (en) * | 2021-04-14 | 2021-07-16 | 四川大学 | Lead ion detection method based on G-quadruplex proportion fluorescence |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN103245652A (en) | Method for detecting lead ions by forming G-quadruplex quenching fluorescent light by using functional nucleic acid | |
| Wang et al. | A naked-eye and ratiometric near-infrared probe for palladium via modulation of a π-conjugated system of cyanines | |
| CN105548109B (en) | A kind of fluorescent detection system and detection method of heavy metal cadmium | |
| CN105928914B (en) | The qualitative checking method of sulfurated hydrogen detection sensor and preparation method thereof, the quantitative detecting method of hydrogen sulfide and intracellular hydrogen sulfide | |
| CN109406475A (en) | Double labelling quick response nucleic acid aptamer probe and its method for detecting aflatoxin B1 | |
| CN106959290A (en) | A kind of Ratio-type rare-earth fluorescent probe and the application for detecting bacillus anthracis biomarker | |
| CN106008343B (en) | Mercury ion fluorescence probe and its preparation method and application based on naphthalimide | |
| CN105131935B (en) | Quick high-selectivity fluorine ion colorimetric probe and preparation method thereof | |
| CN109030436A (en) | Carbon quantum dot is the tyrosinase activity analyzing novel methods of fluorescence probe | |
| CN102375021B (en) | Electrochemical method employing DNA as probe to detect environmental pollutant | |
| Jia et al. | Extended GR-5 DNAzyme-based Autonomous isothermal Cascade machine: An efficient and sensitive one-tube colorimetric platform for Pb2+ detection | |
| CN103305622A (en) | Method for detecting lead by G-quadruplex fluorescence method formed by using unlabeled functional nucleic acid | |
| CN107880034A (en) | A kind of fluorescence probe of Visual retrieval hydrazine based on benzothiazole and its production and use | |
| CN106841050A (en) | One kind is for detecting UO22+Liquid crystal bio-sensing method | |
| Wang et al. | Inhibition of double-stranded DNA templated copper nanoparticles as label-free fluorescent sensors for L-histidine detection | |
| CN107121415A (en) | The method of the label-free fluorescence quick detection mercury ion of single step | |
| CN106370638A (en) | Colorimetric and fluorescent double-signal biosensor for detecting Hg<2+>, and detection method of biosensor | |
| CN108398406B (en) | Biosensor for detecting uracil glycosylase (UDG) and application thereof | |
| CN104316503A (en) | Use and detection method of sensor based on grapheme quantum dot (GQDs) | |
| CN103940797B (en) | Utilize copper ion specific DNA and the method for SYBR Green I Fluorometric assay copper | |
| CN104215618A (en) | Silver ion detection method based on nano-gold accumulation and dispersion quenching Rhodamine B fluorescence | |
| CN108680547B (en) | Application of copper nanocluster as fluorescent probe in specific detection of content of rifampicin drug in solution | |
| CN104677869A (en) | Method for detecting 17bata-estradiol by using resonance scattering spectrometry based on functional nucleic acids | |
| CN108774226A (en) | It is a kind of to be used to detect fluorescence probe of silver ion and the preparation method and application thereof | |
| CN109799197B (en) | Application of cyanine dye in lead ion detection, lead ion detection kit and method |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C12 | Rejection of a patent application after its publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20130814 |