CN103242440B - A kind of Extraction Method for Sericin - Google Patents
A kind of Extraction Method for Sericin Download PDFInfo
- Publication number
- CN103242440B CN103242440B CN201310159100.4A CN201310159100A CN103242440B CN 103242440 B CN103242440 B CN 103242440B CN 201310159100 A CN201310159100 A CN 201310159100A CN 103242440 B CN103242440 B CN 103242440B
- Authority
- CN
- China
- Prior art keywords
- silk gum
- silk
- polyacrylamide
- throw out
- solution
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Abstract
The present invention is applicable to compound and extracts field, and provide a kind of Extraction Method for Sericin, described method comprises: filter silk waste water, and regulate filter after the temperature of solution and pH value, add Polyacrylamide Powder, fully stir, leave standstill after generate throw out; Described throw out distilled water is stirred fused, remove polyacrylamide by underpressure distillation and obtain silk gum coarse meal by spray-drying process; In described silk gum coarse meal, add deionized water, the dehydrated alcohol of pre-freeze at-15 DEG C of temperature, leave standstill after fully stirring; Centrifugal rear generation throw out, dissolves described throw out distilled water, removes ethanol by underpressure distillation; Lyophilize obtains pure silk gum powder.The present invention utilizes polyacrylamide to make silk wastewater sedimentation, save the cost needed for concentrated waste water, organic solvent reacts under low-temperature condition, and the biological activity of sericin is not destroyed, simple, the easy to operate and environmental protection of the method, for the rate of recovery of silk gum and purity all very high.
Description
Technical field
The present invention is applicable to compound and extracts field, particularly relates to a kind of Extraction Method for Sericin.
Background technology
Organic solvent method is from silk waste water, extract one of conventional method of silk gum, and it mainly adds ethanol or acetone and other organic solvent in waste water, destroys the hydration layer around protein water body with this, makes its de-steady sedimentation.
In prior art, the silk broadcloth degumming waste water water yield is very large, will isolate silk gum, first will remove most moisture, make concentrated solution under the effect of organic solvent.Adopt general concentrate drying method, treatment capacity is large, the time is long, and its cost consumption also can increase greatly, and industrial applicibility is not strong.So, a kind of method of effective concentrated silk gum waste water must be had, organic solvent method could be reduced and extract the cost of silk gum in suitability for industrialized production.
Summary of the invention
The invention provides a kind of Extraction Method for Sericin, be intended to solve the rate of recovery of silk gum in prior art and purity and to extract the cost of silk gum high, the problem that industrial applicibility is not strong.
Embodiments provide a kind of Extraction Method for Sericin, said method comprising the steps of:
Filter silk waste water, and regulate filter after the temperature of solution and pH value, add Polyacrylamide Powder, fully stir, leave standstill after generate throw out;
Described throw out distilled water is stirred fused, remove polyacrylamide by underpressure distillation and obtain silk gum coarse meal by spray-drying process;
In described silk gum coarse meal, add deionized water, the dehydrated alcohol of pre-freeze at-15 DEG C of temperature, leave standstill after fully stirring;
Centrifugal rear generation throw out, dissolves described throw out distilled water, removes ethanol by underpressure distillation;
Lyophilize obtains pure silk gum powder.
The embodiment of the present invention makes silk wastewater sedimentation by utilizing polyacrylamide, save the cost needed for concentrated waste water, organic solvent reacts under low-temperature condition, and the biological activity of sericin is not destroyed, simple, the easy to operate and environmental protection of the method, for the rate of recovery of silk gum and purity all very high.
Accompanying drawing explanation
Fig. 1 represents the method flow diagram of the extraction silk gum that the embodiment of the present invention provides.
Embodiment
In order to make object of the present invention, technical scheme and advantage clearly understand, below in conjunction with drawings and Examples, the present invention is further elaborated.Should be appreciated that specific embodiment described herein only in order to explain the present invention, be not intended to limit the present invention.
Fig. 1 shows the method flow of the extraction silk gum that the embodiment of the present invention provides, and details are as follows:
In step S101, filter silk waste water, and regulate filter after the temperature of solution and pH value, add Polyacrylamide Powder, fully stir, leave standstill after generate throw out;
In an embodiment of the present invention, the temperature of filtering rear solution is adjusted to 50-80 DEG C, and pH value is adjusted to 7-9.
Polyacrylamide is high molecular weight water soluble polymer, is insoluble to most of organic solvent, has good flocculence, can reduce the frictional resistance between liquid.Polyacrylamide itself and hydrolysis body thereof do not have toxicity, and the toxicity of polyacrylamide is from its residual monomer acrylamide (AN).Acrylamide is the agent of nervosa intoxicating, has damage neural system, shows human body unable, the symptoms such as ataxia after poisoning.Therefore all there is acrylamide content residual in regulation polyacrylamide Industrial products in hygiene department of various countries, is generally 0.5%-0.05%.When polyacrylamide is used for the purifying treatment aspect of industry and municipal effluent, general permission acrylamide content less than 0.2%, during for direct drinking water process, acrylamide content need below 0.05%.
As a preferred embodiment of the present invention, silk gum wastewater quality 0.5-0.8% after the Polyacrylamide Powder added accounts for and filters, fully stirs 15-20 minute, leaves standstill 2-5 hour afterwards and produces throw out.
In step s 102, throw out is stirred with distilled water fused, removes polyacrylamide by underpressure distillation and obtain silk gum coarse meal by spray-drying process;
Take out throw out, add distilled water and be uniformly mixed, after mixing, strength of solution is about 30%, and polyacrylamide is soluble in water, uses distillation under vacuum to be removed.
In step s 103, in silk gum coarse meal, add deionized water, the dehydrated alcohol of pre-freeze at-15 DEG C of temperature, leave standstill after fully stirring;
In an embodiment of the present invention, the mass ratio of the dehydrated alcohol added, deionized water and silk gum coarse meal is 70: 250: 25, fully stirs fused, then rests on 12-24 hour in the environment of-15 DEG C.
In step S104, centrifugal rear generation throw out, dissolves throw out distilled water, removes ethanol by underpressure distillation;
In an embodiment of the present invention, solution centrifugal 30 seconds through refrigerated centrifuge 5000 revs/min, then with silk gum coarse meal mass ratio be 8: 25 distilled water dissolution precipitation, then ethanol is removed in underpressure distillation.
In step S105, lyophilize obtains pure silk gum powder.
Embodiment one:
1. by silk waste water 100Kg strainer filtering;
2. the solution temperature after filtration is adjusted to 70 DEG C, pH value is adjusted to 8, adds the Polyacrylamide Powder of 0.5kg, fully stirs 20 minutes, then leaves standstill 3 hours;
3. take out the throw out formed in waste water, add distilled water and fully stir, allotment strength of solution is about 30%;
4. remove the polyacrylamide in solution with distillation under vacuum, then obtain silk gum coarse meal 8.42kg by spray-drying process;
5. in silk gum coarse meal, add the dehydrated alcohol 23.7kg of pre-freeze under deionized water 85kg ,-15 DEG C of environment, stir 15 minutes, then to rest in the environment of-15 DEG C 12 hours;
6. solution produces precipitation in centrifugal 30 seconds through refrigerated centrifuge 5000r/min, and then use the distilled water dissolution precipitation of 2.8kg, then ethanol is removed in underpressure distillation;
7. solution lyophilize is obtained pure silk gum powder 7.46kg.
Silk gum extraction rate reached is to more than 85%.With polyacrylamide precipitation silk waste water, produce silk gum coarse meal with spray-drying process drying again after forming throw out, its production cost will well below existing method.So the inventive method, no matter from cost or the angle of efficiency, is all applicable to being applied to suitability for industrialized production.
The embodiment of the present invention makes silk wastewater sedimentation by utilizing polyacrylamide, saves the cost needed for concentrated waste water; Organic solvent reacts under low-temperature condition, and the biological activity of sericin is not destroyed.The inventive method technique does not simply pollute again.Sericin recovery rate is higher, and the silk gum purity extracted is high, good water solubility.
The above is only the preferred embodiment of the present invention; it should be pointed out that for those skilled in the art, under the premise without departing from the principles of the invention; can also make some improvements and modifications, these improvements and modifications also should be considered as protection scope of the present invention.
Claims (1)
1. an Extraction Method for Sericin, is characterized in that, said method comprising the steps of:
By silk waste water 100Kg strainer filtering;
Solution temperature after filtering is adjusted to 70 DEG C, and pH value is adjusted to 8, adds the Polyacrylamide Powder of 0.5kg, fully stirs 20 minutes, then leaves standstill 3 hours;
Take out the throw out formed in waste water, add distilled water and fully stir, allotment strength of solution is 30%;
Remove the polyacrylamide in solution with distillation under vacuum, then obtain silk gum coarse meal 8.42kg by spray-drying process;
In silk gum coarse meal, add the dehydrated alcohol 23.7kg of pre-freeze under deionized water 85kg ,-15 DEG C of environment, stir 15 minutes, then to rest in the environment of-15 DEG C 12 hours;
Solution produces precipitation in centrifugal 30 seconds through refrigerated centrifuge 5000r/min, and then use the distilled water dissolution precipitation of 2.8kg, then ethanol is removed in underpressure distillation;
Solution lyophilize is obtained pure silk gum powder 7.46kg.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310159100.4A CN103242440B (en) | 2013-05-03 | 2013-05-03 | A kind of Extraction Method for Sericin |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310159100.4A CN103242440B (en) | 2013-05-03 | 2013-05-03 | A kind of Extraction Method for Sericin |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN103242440A CN103242440A (en) | 2013-08-14 |
| CN103242440B true CN103242440B (en) | 2015-09-16 |
Family
ID=48922343
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201310159100.4A Expired - Fee Related CN103242440B (en) | 2013-05-03 | 2013-05-03 | A kind of Extraction Method for Sericin |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN103242440B (en) |
Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104592377B (en) * | 2014-12-29 | 2018-02-27 | 无锡蚕魅科技有限公司 | The extracting method of silk gum in a kind of silk cocoon |
| CN108503703A (en) * | 2018-05-25 | 2018-09-07 | 江苏澳特丝生物科技有限公司 | A kind of extracting method of Sericin from Silk Wastewater |
| CN114804494A (en) * | 2022-06-13 | 2022-07-29 | 滨海宇美科技有限公司 | Preparation method for recovering sericin from silk reeling waste liquid |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101182062A (en) * | 2007-11-30 | 2008-05-21 | 重庆工学院 | Method for treating waste water containing sericin protein |
| CN102585285A (en) * | 2012-01-17 | 2012-07-18 | 苏州大学 | Recovery method of silk glue and silk glue |
| CN102887946A (en) * | 2012-09-18 | 2013-01-23 | 南宁专精特高新技术咨询服务有限公司 | Method for extracting sericin from cocoon cooking wastewater by physical method |
-
2013
- 2013-05-03 CN CN201310159100.4A patent/CN103242440B/en not_active Expired - Fee Related
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101182062A (en) * | 2007-11-30 | 2008-05-21 | 重庆工学院 | Method for treating waste water containing sericin protein |
| CN102585285A (en) * | 2012-01-17 | 2012-07-18 | 苏州大学 | Recovery method of silk glue and silk glue |
| CN102887946A (en) * | 2012-09-18 | 2013-01-23 | 南宁专精特高新技术咨询服务有限公司 | Method for extracting sericin from cocoon cooking wastewater by physical method |
Non-Patent Citations (1)
| Title |
|---|
| 物理化学法处理绢纺脱胶废水的研究;董雪等;《丝绸》;20110831;第48卷(第8期);摘要，第23页左栏倒数第2段，第23页右栏最后一段，第25页左栏第1段、图5，第26页左栏倒数第2段 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN103242440A (en) | 2013-08-14 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN104342040B (en) | A kind of high glue melts the preparation method of temperature fish scale gelatin | |
| CN102488713B (en) | Method for preparing sheep placenta extract and sheep placenta hydrolyzed collagen concentrated solution | |
| CN103242440B (en) | A kind of Extraction Method for Sericin | |
| CN104177508A (en) | Method for comprehensively extracting tea seed saponin, tea seed polypeptide and tea seed polysaccharide from tea seed cake | |
| CN101575371B (en) | Method for adopting tannin to process silkworm cocoon processing wastewater and recycle sericin protein | |
| CN100535110C (en) | Novel extraction method of bromelain | |
| CN101012340A (en) | Method of extracting and purifying melanin from almond peel | |
| CN103342669A (en) | Simple method for extracting natural taurine | |
| CN102887946A (en) | Method for extracting sericin from cocoon cooking wastewater by physical method | |
| CN103305492A (en) | Method for extracting high-purity pharmaceutical grade bromelain | |
| CN105692850A (en) | Preparation technique of banana peel polymer coagulant for water treatment | |
| CN103275948B (en) | Production process for extracting four enzymes from cattle or pig pancreas | |
| CN103623618B (en) | A kind of take quartz sand as the preparation method of the Hydrophobic filter material of carrier | |
| CN101805773A (en) | Method for producing egg membrane protein | |
| CN104558229A (en) | Separating and purifying method for Chinese wolfberry polyose | |
| CN101671400A (en) | Method of preparing soluble soybean polysaccharide | |
| CN108486199B (en) | Water-soluble chelating agent, preparation method thereof and heavy metal wastewater treatment method using water-soluble chelating agent | |
| CN103059159A (en) | Process for extracting mannan from beer yeast powder | |
| CN102617752A (en) | Production process of low molecular weight pectin | |
| CN104558136A (en) | Method for obtaining sericin from silk reeling wastewater | |
| CN103289968B (en) | From the water-soluble extracting liquid of sweet potato, be separated and prepare the method for polyphenoloxidase, beta-amylase and storage protein | |
| CN103864911A (en) | Method for recovering water-soluble protein in minced fillet rinse water by using composite flocculant | |
| CN103588368A (en) | Biomaterial for sludge decrement treatment | |
| CN101780118B (en) | Method for extracting active ingredients of artificially cultured cordceps militaris waste liquor by flocculation | |
| CN103387501A (en) | Method for preparing high-purity L-synephrine |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20150916 Termination date: 20160503 |