CN103239380A - Application of traditional Chinese medicine, screening method and anti-ageing drug thereof - Google Patents
Application of traditional Chinese medicine, screening method and anti-ageing drug thereof Download PDFInfo
- Publication number
- CN103239380A CN103239380A CN2013101867349A CN201310186734A CN103239380A CN 103239380 A CN103239380 A CN 103239380A CN 2013101867349 A CN2013101867349 A CN 2013101867349A CN 201310186734 A CN201310186734 A CN 201310186734A CN 103239380 A CN103239380 A CN 103239380A
- Authority
- CN
- China
- Prior art keywords
- chinese medicine
- loxl1
- traditional chinese
- screening
- rhizoma
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Images
Landscapes
- Cosmetics (AREA)
- Medicines Containing Plant Substances (AREA)
Abstract
The invention discloses application of a traditional Chinese medicine, a screening method and an anti-ageing drug thereof. The traditional Chinese medicine has the effect of promoting LOXL1 genetic transcription; the traditional Chinese medicine is used for preparing a cosmetic additive and a feed additive, or used for preparing a drug for treating or controlling diseases caused by dynamic connective tissue lesions; and the traditional Chinese medicine comprises radix curcumae, rheum officinale, radix curcumae longae, barbados aloe, giant knot weed. Extract molecules of these traditional Chinese medicines have the effect of promoting LOXL1 genetic transcription, so as to promote crosslinking of extracellular matrix.
Description
Technical field
The present invention relates to biomedicine field, relate in particular to a kind of application and screening technique and antiaging agent of Chinese medicine.
Background technology
With advancing age, the elastic forfeiture of body causes that the increase of wrinkle of skin is old and feeble typical characteristic.Therefore the molecular screening of delaying decrepitude of skin can screen and can improve the elastic molecule of body and realize.Known now, body elasticity is mainly provided by the crosslinked elastic fiber of high-sequential on the space, and wherein the degree of cross linking of elastic fiber is the elastic leading indicator of assessment.Early than 2004 and 2006, discover, rely acyl group oxidase 1(Lysyl oxidase-like 1, LOXL1) be the key factor of safeguarding growth and the renewal thereof of body elastic fiber specifically.There is not LOXL1 directly to influence being cross-linked to form of elastic fiber specifically, and then cause the pathological changes of a series of dynamic connective tissue, comprise macula retinae regression, sexual organ's prolapsus and urinary incontinence that cutis laxa, emphysema, Cardiovascular abnormality, blood vessel hyperplasia bring out.Discover that further along with wearing out of histoorgan, the level of LOXL1 reduces greatly, and is accompanied by the decline of the elastic fiber degree of cross linking and the increase of the unordered accumulation of elastin laminin monomer.These discoveries disclose the aging course that can delay the biologic artifact extracellular matrix by the expression of enhancing LOXL1 albumen or activity, and (Nature Genetics 2004 (36): 178; Am J Path 2006(168): 519; IOVS 2008(49): 2599; WO/2005/069975, US Patent 7255856, US Patent 7255857, European patent 1706139).
But prior art has or not and a kind of drug screening method occurs, can be used for filtering out to the expression of LOXL1 albumen or the material of active effective facilitation, and with this type of material for the preparation of antidotal medicine, cosmetics etc.In addition, be used for antidotal medicine in the prior art, its active component has adopted a certain amount of chemical synthetic material.These chemical synthetic materials have certain zest to skin, toxic and side effects in various degree.And, can not solve the skin aging problem from gene level.
Therefore, prior art has yet to be improved and developed.
Summary of the invention
In view of above-mentioned the deficiencies in the prior art, the object of the present invention is to provide a kind of application and screening technique and antiaging agent of Chinese medicine, be intended to solve existing antiaging agent problem.
Technical scheme of the present invention is as follows:
A kind of application of Chinese medicine, wherein, described Chinese medicine has the into effect of LOXL1 genetic transcription, with described Chinese medicine for the preparation of cosmetics additive, food additive, or for the preparation for the treatment of or improve medicine because of the disease due to the dynamic connective tissue pathological changes.
The application of described Chinese medicine, wherein, described Chinese medicine is Radix Curcumae, Radix Et Rhizoma Rhei, Rhizoma Curcumae Longae, Aloe, Rhizoma Polygoni Cuspidati.
A kind of antiaging agent wherein, contains the extract of aforesaid Chinese medicine in the described antiaging agent.
Described antiaging agent, wherein, described Chinese medicine is Radix Curcumae, Radix Et Rhizoma Rhei, Rhizoma Curcumae Longae, Aloe, Rhizoma Polygoni Cuspidati.
A kind of method of screening of medicaments wherein, is used for screening and has the into medicine of the effect of LOXL1 genetic transcription, and the method for described screening of medicaments may further comprise the steps:
Grind the Chinese medicine medical material, with the ethanol refluxing process extracting for several times, filter and merging filtrate lyophilization; The extract powder is joined in the normal saline, preparation extract solution joins in the cell culture system to be measured, after cultivating 24-48 hour, method by PCR in real time detects LOXL1 mRNA level, and detects the LOXL1 protein level by immunoblot experiment.
The method of described screening of medicaments, wherein, described screening technique, further comprising the steps of:
After 110 ℃ of digestion are spent the night through 1N hydrochloric acid with the cell to be measured cultivated, detect the content of desmosine, determine by the content of desmosine whether the elastic fiber degree of cross linking improves.
Beneficial effect: the application of described Chinese medicine is provided among the present invention, described Chinese medicine has the effect that promotes the LOXL1 genetic transcription, with described medicine for the preparation of cosmetics additive, food additive, or for the preparation for the treatment of or improve medicine because of the disease due to the dynamic connective tissue pathological changes.Described Chinese medicine has the raising skin elasticity, the effect of delaying decrepitude of skin.And the present invention also provides a kind of screening technique of medicine of the LOXL1 of promotion genetic transcription, filters out to have the crosslinked Chinese medicine of the extracellular matrix of promotion, and the method is simple to operate, the Chinese medicine that can fast detecting goes out to have facilitation.
Description of drawings
Fig. 1 is the LOXL1 mRNA horizontal detection result of human fibroblasts in the embodiment of the invention 1.
Fig. 2 is the LOXL1 protein level testing result of human fibroblasts in the embodiment of the invention 1.
Fig. 3 is the LOXL1 desmosine content detection result of human fibroblasts in the embodiment of the invention 2.
Fig. 4 is the histiocytic LOXL1 mRNA of application on human skin horizontal detection result in the embodiment of the invention 3.
Fig. 5 is the histiocytic LOXL1 protein level of application on human skin testing result in the embodiment of the invention 3.
Fig. 6 is the histiocytic LOXL1 desmosine of application on human skin content detection result in the embodiment of the invention 4.
The specific embodiment
The invention provides a kind of application and screening technique and antiaging agent of Chinese medicine, clearer, clear and definite for making purpose of the present invention, technical scheme and effect, below the present invention is described in more detail.Should be appreciated that specific embodiment described herein only in order to explaining the present invention, and be not used in restriction the present invention.
For seeking the drug molecule that can improve LOXL1 level in the body, the present invention is by the method for PCR in real time, find a series of Chinese medicines that can promote the LOXL1 genetic transcription, these Chinese medicines have been acted on histiocyte, thereby improved LOXL1 albumen in the cell and the degree of cross linking of elastic fiber.
Described Chinese medicine is Radix Curcumae, Radix Et Rhizoma Rhei, Rhizoma Curcumae Longae, Aloe, Rhizoma Polygoni Cuspidati.The extract molecule of these Chinese medicines all has the effect that can promote the LOXL1 genetic transcription, thereby promotes that extracellular matrix is crosslinked.Wherein, the facilitation of Rhizoma Polygoni Cuspidati is the most outstanding.Therefore, the invention provides a kind of application of Chinese medicine, described Chinese medicine has the into effect of LOXL1 genetic transcription, with described Chinese medicine for the preparation of cosmetics additive, food additive, or for the preparation for the treatment of or improve medicine because of the disease due to the dynamic connective tissue pathological changes.
Because the extract of this type of Chinese medicine can be by improving the mode of the extracellular matrix elastic fiber degree of cross linking, with delaying decrepitude of skin.Therefore also provide a kind of antiaging agent among the present invention, contain the extract with the Chinese medicine that promotes the LOXL1 genetic transcription in the described antiaging agent.
The screening technique of described Chinese medicine also is provided among the present invention, by simple steps, effectively filters out the Chinese medicine with promotion LOXL1 genetic transcription rapidly.Described screening technique may further comprise the steps:
Grind an amount of Chinese medicine medical material, with the ethanol refluxing process extracting for several times, filter and merging filtrate lyophilization; The extract powder is joined in the normal saline, preparation extract solution joins in the cell culture system to be measured, after cultivating 24-48 hour, method by PCR in real time detects LOXL1 mRNA level, and detects the LOXL1 protein level by immunoblot experiment.
Like this, by PCR in real time and immunoblot experiment double check, determine whether Chinese medicine has the effect that promotes the LOXL1 genetic transcription.Whether the elastic fiber degree of cross linking improves after described medicine extract is handled in order further to assess, and described screening technique is further comprising the steps of:
After 110 ℃ of digestion are spent the night through 1N hydrochloric acid with the cell to be measured cultivated, detect the content of desmosine, determine by the content of desmosine whether the elastic fiber degree of cross linking improves.
Wherein, described cell to be measured can be human fibroblasts or application on human skin histiocyte.
The present invention will be further described by the following examples.
Embodiment 1: the drug screening of LOXL1 gene expression in the preparation of Chinese medicine crude extract and the stimulation human fibroblasts
Grind an amount of medical material, with the ethanol refluxing process extracting for several times, filter and merging filtrate lyophilization.The crude extract powder is joined in the normal saline, and preparation concentration is the liquid storage of 1 mg/ml.The medicine crude extract joins in the human fibroblasts cultivating system with 200 times of dilutions during use, cultivates and detects LOXL1 mRNA level and protein level in 24 hours afterwards.LOXL1 mRNA horizontal detection result as shown in Figure 1, the LOXL1 protein level is as shown in Figure 2.PCR in real time result shows that Radix Curcumae, Radix Et Rhizoma Rhei, Rhizoma Curcumae Longae, Aloe, Rhizoma Polygoni Cuspidati extract can improve LOXL1 mRNA level by significance; Immunoblot experiment shows that the protein level of LOXL1 has increased 1.3-2.1 doubly, and illustrated is meansigma methods ± SEM(n=6; P＜0.01).
Embodiment 2: the Chinese medicine crude extract is to the crosslinked facilitation of elastic fiber in the human fibroblasts
Be assessment elastic fiber degree of cross linking after drug treating, measured human fibroblasts in the present embodiment through the spend the night content of desmosine of the afterreaction elastic fiber degree of cross linking of 110 ℃ of digestion of 1N hydrochloric acid, testing result as shown in Figure 3.The result shows after Radix Curcumae, Radix Et Rhizoma Rhei, Rhizoma Curcumae Longae, Aloe, Rhizoma Polygoni Cuspidati extract are handled cell 24 hours, and the desmosine content of cell has improved 0.4-1.7 doubly, and the degree of cross linking significance raising of elastic fiber is described, illustrated is meansigma methods ± SEM(n=6; P＜0.01).
Example 3: the facilitation that the Chinese medicine extract is expressed LOXL1 in the application on human skin tissue
Further measured after the application on human skin tissue culture 48 hours the influence that 200 times of dilutions of medicine are expressed LOXL1 in the present embodiment.LOXL1 mRNA horizontal detection result as shown in Figure 4, the LOXL1 protein level is as shown in Figure 5.The result of PCR in real time shows that LOXL1 mRNA level has improved 0.4-1.9 doubly; The experiment of immunoblotting shows that the LOXL1 protein level has improved 0.7-1.6 doubly, and illustrated is meansigma methods ± SEM(n=6; P＜0.01).
Embodiment 4: the Chinese medicine extract is to the crosslinked facilitation of elastic fiber in the application on human skin tissue
Further measured after the application on human skin tissue culture 48 hours the elastic fiber degree of cross linking of cell in the present embodiment.The application on human skin tissue detects the content of the desmosine of the elastic fiber degree of cross linking after 110 ℃ of digestion of 1N hydrochloric acid are spent the night, the result as shown in Figure 6.The result shows that after Radix Curcumae, Radix Et Rhizoma Rhei, Rhizoma Curcumae Longae, Aloe, Rhizoma Polygoni Cuspidati extract were handled, desmosine content had improved 0.5-1.8 doubly, illustrates that the degree of cross linking significance of elastic fiber improves, and illustrated is meansigma methods ± SEM(n=6; P＜0.01).
To sum up, the invention provides a kind of screening technique of medicine of the LOXL1 of promotion genetic transcription, filter out to have and promote the crosslinked Chinese medicine of extracellular matrix, the method is simple to operate, the Chinese medicine that can fast detecting goes out to have facilitation.And the application of described Chinese medicine also is provided among the present invention, with described medicine for the preparation of cosmetics additive, food additive, or for the preparation for the treatment of or improve medicine because of the disease due to the dynamic connective tissue pathological changes.Described Chinese medicine has the raising skin elasticity, the effect of delaying decrepitude of skin.
Should be understood that application of the present invention is not limited to above-mentioned giving an example, for those of ordinary skills, can be improved according to the above description or conversion that all these improvement and conversion all should belong to the protection domain of claims of the present invention.
Claims (6)
1. the application of a Chinese medicine, it is characterized in that, described Chinese medicine has the into effect of LOXL1 genetic transcription, with described Chinese medicine for the preparation of cosmetics additive, food additive, or for the preparation for the treatment of or improve medicine because of the disease due to the dynamic connective tissue pathological changes.
2. the application of Chinese medicine according to claim 1 is characterized in that, described Chinese medicine is Radix Curcumae, Radix Et Rhizoma Rhei, Rhizoma Curcumae Longae, Aloe, Rhizoma Polygoni Cuspidati.
3. an antiaging agent is characterized in that, contains the extract of Chinese medicine as claimed in claim 1 in the described antiaging agent.
4. antiaging agent according to claim 3 is characterized in that, described Chinese medicine is Radix Curcumae, Radix Et Rhizoma Rhei, Rhizoma Curcumae Longae, Aloe, Rhizoma Polygoni Cuspidati.
5. the method for a screening of medicaments is characterized in that, is used for screening and has the into medicine of the effect of LOXL1 genetic transcription, and the method for described screening of medicaments may further comprise the steps:
Grind the Chinese medicine medical material, with the ethanol refluxing process extracting for several times, filter and merging filtrate lyophilization; The extract powder is joined in the normal saline, preparation extract solution joins in the cell culture system to be measured, after cultivating 24-48 hour, method by PCR in real time detects LOXL1 mRNA level, and detects the LOXL1 protein level by immunoblot experiment.
6. according to the method for the described screening of medicaments of claim 5, it is characterized in that, described screening technique, further comprising the steps of:
After 110 ℃ of digestion are spent the night through 1N hydrochloric acid with the cell to be measured cultivated, detect the content of desmosine, determine by the content of desmosine whether the elastic fiber degree of cross linking improves.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2013101867349A CN103239380A (en) | 2013-05-20 | 2013-05-20 | Application of traditional Chinese medicine, screening method and anti-ageing drug thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2013101867349A CN103239380A (en) | 2013-05-20 | 2013-05-20 | Application of traditional Chinese medicine, screening method and anti-ageing drug thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN103239380A true CN103239380A (en) | 2013-08-14 |
Family
ID=48919487
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2013101867349A Pending CN103239380A (en) | 2013-05-20 | 2013-05-20 | Application of traditional Chinese medicine, screening method and anti-ageing drug thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN103239380A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103249842A (en) * | 2010-11-30 | 2013-08-14 | 株式会社爱茉莉太平洋 | Method for screening skin aging-elated genes and materials for preventing skin aging |
| US20200232972A1 (en) * | 2017-07-18 | 2020-07-23 | Shiseido Company, Ltd. | Method for screening anti-aging substances |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1766034A (en) * | 2005-10-28 | 2006-05-03 | 广西师范大学 | Giant knotweed rhizome general anthraquinone and uses and formulation of acid ingredient of giant knotweed rhizome general anthraquinone |
| CN101069735A (en) * | 2007-03-29 | 2007-11-14 | 广西师范学院 | Turmeric volatile-oil extraction and curcumin chemical compounds purifying method |
| CN101147575A (en) * | 2006-09-22 | 2008-03-26 | 楼飞 | Toxin-expelling intestine-cleaning health-care food composition and preparation method thereof |
| CN101336878A (en) * | 2008-08-14 | 2009-01-07 | 温州医学院生物与天然药物开发中心有限公司 | Extraction method of common turmeric powder and use thereof in cosmetics |
| CN102965425A (en) * | 2012-10-26 | 2013-03-13 | 上海市第十人民医院 | Medicament screening method, medicament used for promoting crosslink of extracellular matrix and application of medicament |
-
2013
- 2013-05-20 CN CN2013101867349A patent/CN103239380A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1766034A (en) * | 2005-10-28 | 2006-05-03 | 广西师范大学 | Giant knotweed rhizome general anthraquinone and uses and formulation of acid ingredient of giant knotweed rhizome general anthraquinone |
| CN101147575A (en) * | 2006-09-22 | 2008-03-26 | 楼飞 | Toxin-expelling intestine-cleaning health-care food composition and preparation method thereof |
| CN101069735A (en) * | 2007-03-29 | 2007-11-14 | 广西师范学院 | Turmeric volatile-oil extraction and curcumin chemical compounds purifying method |
| CN101336878A (en) * | 2008-08-14 | 2009-01-07 | 温州医学院生物与天然药物开发中心有限公司 | Extraction method of common turmeric powder and use thereof in cosmetics |
| CN102965425A (en) * | 2012-10-26 | 2013-03-13 | 上海市第十人民医院 | Medicament screening method, medicament used for promoting crosslink of extracellular matrix and application of medicament |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103249842A (en) * | 2010-11-30 | 2013-08-14 | 株式会社爱茉莉太平洋 | Method for screening skin aging-elated genes and materials for preventing skin aging |
| CN103249842B (en) * | 2010-11-30 | 2016-07-06 | 株式会社爱茉莉太平洋 | Screen the gene relevant with skin aging and prevent the method for material of skin aging |
| US20200232972A1 (en) * | 2017-07-18 | 2020-07-23 | Shiseido Company, Ltd. | Method for screening anti-aging substances |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Borthakur et al. | The probiotic Lactobacillus plantarum counteracts TNF-α-induced downregulation of SMCT1 expression and function | |
| EP2627343B1 (en) | Agents for use for promoting the synthesis of collagen and proteoglycan in intervertebral disc cells | |
| Sun et al. | Chondroitin sulfate from sturgeon bone ameliorates pain of osteoarthritis induced by monosodium iodoacetate in rats | |
| CN107375148B (en) | A kind of skin repair composition | |
| Mao et al. | GelNB molecular coating as a biophysical barrier to isolate intestinal irritating metabolites and regulate intestinal microbial homeostasis in the treatment of inflammatory bowel disease | |
| CN110101740A (en) | A kind of composition and its preparation of skin repair | |
| Chen et al. | The inhibitory effects of pudendal nerve stimulation on bladder overactivity in spinal cord injury dogs: is early stimulation necessary? | |
| CN109172613B (en) | Skin external composition containing lactobacillus dead bacteria culture and its use for promoting wound healing and reducing scar generation | |
| CN105662941A (en) | Immature bitter orange extract product and preparation method thereof and application of immature bitter orange extract product for delaying skin aging | |
| CN109069588A (en) | Composition comprising GDF11 and its purposes | |
| Carotenuto et al. | Dietary flaxseed mitigates impaired skeletal muscle regeneration: in vivo, in vitro and in silico studies | |
| TW202014176A (en) | Pachyrhizus erosus fermented extracts and the use thereof for enhancing the gene expression of col, timp, lox, eln, has, sod, tcp1 and ung, and for reducing the skin melanin content | |
| CN103239380A (en) | Application of traditional Chinese medicine, screening method and anti-ageing drug thereof | |
| CN110664995B (en) | Composition containing recombinant human fibronectin peptide | |
| CN102965425A (en) | Medicament screening method, medicament used for promoting crosslink of extracellular matrix and application of medicament | |
| CN108392508A (en) | Application of the white lotus parthenium extract in preparing anti-inflammatory drug | |
| CN108379284A (en) | Purposes of the black tiger palm dietary fiber extract in preparing treatment and/or preventing intestinal bacilli illness relevant disease preparation | |
| Im et al. | Wrinkle reduction using a topical herbal cream in subjects with greater yin (Tae-eumin) type: A randomized double-blind placebo-controlled study | |
| CN100341574C (en) | Novel use of cell wall skeleton of red nocar-ray-fungus for treating liver diseases | |
| CN106381279A (en) | Extracellular polymeric substances, preparation method and application thereof | |
| CN113476362B (en) | Facial skin cutin renewal promoter and application thereof | |
| CN102631361B (en) | Application of oleanane glycoside in preparation of drugs for treating and/or preventing schistosomiasis | |
| CN106822169A (en) | Application of the cordycepin in the medicine for preparing prevention and/or radiotherapy damage and preparing tumor radiotherapy enhanced sensitivity | |
| CN106754139A (en) | A kind of health liquor and preparation method for increasing aldehyde dehydrogenase activity | |
| CN106999531A (en) | Composition for lymph discharge opeing |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C12 | Rejection of a patent application after its publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20130814 |