CN103233024B - Paddy rice lateral root density-related coding gene and use thereof - Google Patents
Paddy rice lateral root density-related coding gene and use thereof Download PDFInfo
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- CN103233024B CN103233024B CN201310073138.XA CN201310073138A CN103233024B CN 103233024 B CN103233024 B CN 103233024B CN 201310073138 A CN201310073138 A CN 201310073138A CN 103233024 B CN103233024 B CN 103233024B
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Abstract
The invention discloses a paddy rice lateral root density-related coding gene and a use thereof. The paddy rice lateral root density-related coding gene is a T-type allele of an NCED gene. The T-type allele of the NCED gene can obviously improve upland rice endogenous ABA content, increase the number of lateral root, improve drought resistance of upland rice and improve an upland rice yield under the drought condition.
Description
Technical field
The invention belongs to molecular biology and agriculture field, the encoding gene relevant in particular to a kind of Rice lateral root density and application thereof.
Background technology
The artificial selection of farm crop generally experienced by domestication and improvement 2 processes, and domestication process is generally that wild species are become easy to raise and results, and improved, process then focuses on the economic characters of crop more, as output, quality, resistance, adaptability etc.Excavate and clone in crop and have great importance with the relevant gene of improvement, by the genetic resources improved farm crop for us and provide important.
All the time, Arid Problem is one of principal element affecting crop yield always.At present, the drought resisting problem of reply crop particularly paddy rice, the research of the overwhelming majority is the natural population based on paddy rice, by some genes such as transcription factor, protein kinase of process LAN, reaches the object of corresponding drought resisting.
Summary of the invention
The invention provides the relevant encoding gene of a kind of Rice lateral root density and application thereof, this gene can improve Rice lateral root density.In order to realize object of the present invention, intend adopting following technical scheme:
The gene of 9-cis-epoxy carotenoid dioxygenase (Nced) is numbered Os12g0435200 (its gene order is SEQ ID NO.:C), the gene of wild-type is G instead of A the base of 1009, therefore be C type (complementary base is G) by the NCED unnamed gene of wild-type, the present invention forms the allelotrope with drought resistance function by the bases G of 1009 being replaced with A, its gene order is SEQ ID NO.:1, and by T-shaped for its called after, this allelotrope causes the amino acid of gene encoding enzyme to become Isoleucine from α-amino-isovaleric acid.
One aspect of the present invention relates to a kind of encoding gene, and it comprises the gene order of SEQ ID NO.:1 or the gene order of its complementation.
In another aspect of this invention, the invention still further relates to above-mentioned albumen expressed by above-mentioned encoding gene.
The present invention also relates on the other hand carrier containing above-mentioned encoding gene or the application of paddy rice in breeding.
The carrier that the invention still further relates to containing above-mentioned encoding gene is improving the application in Rice lateral root density, preferably, is also included in the effect improved in Rice Drought Resistence ability.
The T-shaped allelotrope of Nced gene of the present invention significantly can increase the endogenous ABA content of dryland rice, and makes the increased number of side root, thus makes dryland rice drought resisting more, thus can improve the output of dryland rice under drought stress.
Accompanying drawing explanation
The ABA content of Fig. 1: 2 kinds of genotype dryland rice;
Fig. 2: F7 RIL 2 kinds genotype family ABA content;
The side root density of Fig. 3: 2 kinds of genotype dryland rice;
Root system photo under Fig. 4: 2 kinds of genotype dryland rice stereoscopes;
The side root density of Fig. 5: F7 RIL 2 kinds of genotype familys;
Root system photo under Fig. 6: F7 RIL 2 kinds genotype stereoscope.
Embodiment:
Embodiment 1
Enzyme linked immunological (ELISA) method measures ABA:
T-shaped and the genotypic dryland rice of C type (gene in C type and TXing Junshi natural population) nourishing and growing period (now Nced gene is high expression level) after dryland rice tillers is planted in the control water surrounding in greenhouse, gather the blade (each 0.5g) of 20 T-shaped dryland rice and 17 C type dryland rice, use euzymelinked immunosorbent assay (ELISA) to carry out the mensuration of hormone ABA content.All samples are clayed into power in liquid nitrogen, methyl alcohol (2,6 di tert butyl 4 methyl phenol containing the 1mmol/L) extracted overnight with 80%.By centrifugal for mixed solution 6000rpm 15 minutes, use nitrogen to dry up supernatant, then dissolve in phosphate buffered saline buffer (pH=7.5,1%Tween-20,1%glutin).Measure as shown in Figure 1, the ABA content A of T-shaped dryland rice is significantly higher than the ABA content (t-test, P=0.033) of C type dryland rice.
In addition, use C type I in Rice R64 and T-shaped dryland rice IRAT104 to construct F7 RIL isolates from generation to generation, this isolates comprises 11 T-shaped familys and 12 C type familys.Determine the ABA content of 2 kinds of familys respectively, as shown in Figure 2, the ABA content of T-shaped family will be significantly higher than the ABA content (t-test, P=0.016) of C type family.
By above 2 experiments, such conclusion can be drawn: the T-shaped allelotrope of NCED, compared with the C type of wild-type, can significantly improve the activity of 9-cis-epoxy carotenoid dioxygenase, thus the content of hormone ABA is increased.
2. the dryland rice strain of different genotype and the side root of RIL strain is quantitative
Under the condition of greenhouse simulation dryland rice growing environment, 9 kinds of C type dryland rice and 8 kinds of T-shaped dryland rice strains are planted.When growing to the vegetative growth stage after tillering, it is taken out from soil and root is cleaned up (this process will be avoided hurting root system system) as far as possible.Measure all main root length, added up the number of side root main root being longer than 1cm, and calculate the density (the main root total length of side root overall number/each plant of each plant) of side root.Each strain we respectively added up 5 individual plants and calculated mean value.We find, the side root mean number on T-shaped dryland rice unit length main root to be significantly higher than C type dryland rice number (t-test, P=0.009, as shown in Figure 3).Fig. 4 display be the picture of root system under stereoscope of C type dryland rice and T-shaped dryland rice.Simultaneously, we make to use the same method and the F7 built IR64 and IRAT104 have been carried out to the mensuration of root system phenotype for the family of RIL different genotype, as shown in Figure 5, on the upside of T-shaped family unit main root, the average number of root will be significantly higher than (t-test, the P=0.035) of C type family.What Fig. 6 showed is the phenotype of the root of 2 kinds of genotype familys.
By the test of above root system phenotype test, conclusion that can be such: the side root density of T-shaped dryland rice will be significantly higher than the side root density of C type dryland rice.
The above, be only the specific embodiment of the present invention, but protection scope of the present invention is not limited thereto, and any change of expecting without creative work or replacement, all should be encompassed within protection scope of the present invention.Therefore, the protection domain that protection scope of the present invention should limit with claims is as the criterion.
Claims (7)
1. an encoding gene, its sequence is the gene order of SEQ ID NO.1 or the gene order of its complementation.
2. containing encoding gene described in claim 1 can expression vector.
3. encoding gene according to claim 1 or according to claim 2 can albumen expressed by expression vector.
4. the carrier containing encoding gene according to claim 1 or the application of rice paddy seed in rice breeding.
5. the carrier containing encoding gene according to claim 1 or the application of rice paddy seed in rice breeding, described paddy rice is dryland rice.
6. the application of the carrier containing encoding gene according to claim 1 in the side root density improving paddy rice.
7. the carrier containing encoding gene according to claim 1 is improving the application in Rice Drought Resistence ability.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201310073138.XA CN103233024B (en) | 2013-03-07 | 2013-03-07 | Paddy rice lateral root density-related coding gene and use thereof |
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| CN201310073138.XA CN103233024B (en) | 2013-03-07 | 2013-03-07 | Paddy rice lateral root density-related coding gene and use thereof |
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| CN103233024A CN103233024A (en) | 2013-08-07 |
| CN103233024B true CN103233024B (en) | 2015-03-04 |
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Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN103484472B (en) * | 2013-08-23 | 2015-04-29 | 宁波大学 | Rice lateral root formation control gene OsHK1 and coded protein thereof |
| CN106701958A (en) * | 2017-01-13 | 2017-05-24 | 云南大学 | Molecular marker of rice drought-resistant gene and application of molecular marker |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1795267A (en) * | 2003-03-24 | 2006-06-28 | 独立行政法人国际农林水产业研究中心 | Stress-induced promoter and method of using the same |
| CN102268081A (en) * | 2011-07-20 | 2011-12-07 | 浙江大学 | Rice lateral root control gene OsIAAII and use thereof |
| CN102584970A (en) * | 2012-02-13 | 2012-07-18 | 浙江大学 | Rice lateral root formation regulatory gene OsLRD1 and protein coded by same |
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Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1795267A (en) * | 2003-03-24 | 2006-06-28 | 独立行政法人国际农林水产业研究中心 | Stress-induced promoter and method of using the same |
| CN102268081A (en) * | 2011-07-20 | 2011-12-07 | 浙江大学 | Rice lateral root control gene OsIAAII and use thereof |
| CN102584970A (en) * | 2012-02-13 | 2012-07-18 | 浙江大学 | Rice lateral root formation regulatory gene OsLRD1 and protein coded by same |
Non-Patent Citations (2)
| Title |
|---|
| Dian.W.M.."GenBank Accession No:AY838898.1".《GenBank》.2004,第1-2页. * |
| Tanaka,T. 等."GenBank Accession No:NM_001073218.1".《GenBank》.2006,第1-4页. * |
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