CN1032318A - Medical profound hypothermia storage bag - Google Patents
Medical profound hypothermia storage bag Download PDFInfo
- Publication number
- CN1032318A CN1032318A CN 87106714 CN87106714A CN1032318A CN 1032318 A CN1032318 A CN 1032318A CN 87106714 CN87106714 CN 87106714 CN 87106714 A CN87106714 A CN 87106714A CN 1032318 A CN1032318 A CN 1032318A
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- bag
- storage bag
- profound hypothermia
- ethylene
- medical
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- Medical Preparation Storing Or Oral Administration Devices (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention provides a kind of profound hypothermia (196 ℃) biological cell and organize storage bag, it is with ethylene-ethyl acetate copolymer or ethylene-ethyl acrylate copolymer blow molding, through the synthetic bag of high frequency heat.This storage bag has good resistance to low temperature (196 ℃), chemical property is stable, machine-shaping is convenient, no toxic substance discharges in the course of processing, and cost of product only is 1/10～1/15 of a fluoride plastic bag of the same type, the low temperature or the profound hypothermia that are applicable to unicellular, haemocyte, embryo, tissue and organ etc. are preserved, for the needs of clinical transplantation and experiment.
Description
The present invention relates to a kind of medical profound hypothermia cell, organize storage bag.
Usually, the control and the adjusting of nerve and body fluid have just been lost after cell, tissue and organ exsomatize.Along with the prolongation of holding time, metabolism is proceeded, and nutriment such as sugar will be consumed to the greatest extent.In order to suppress cellular metabolism, prolong cell life, help long preservation, it is under profound hypothermia (196 ℃) condition.Need a kind of suitable container of anti-the profound hypothermia, and this container preferably has certain diaphaneity, so that observe for this reason.General commercially available polyvinylchloride bag can only tolerate-80 ℃ low temperature at the most, obviously can not meet above-mentioned requirements.Tend to the profound hypothermia storage bag that adopts fluoroplastic to make abroad, it is the fluoride plastic bag of F-46 that domestic also someone has manufactured experimently the trade mark, though this material physicochemical property is good, but price very expensive (price of 100 milliliters of bags is a 20-30 unit), processed complex, forming temperature height (mouthful mould needs more than 380 ℃), thereby it is higher to the material requirements of equipment, and the intermediate that produces in the high temperature process process is poison extremely, and special draft equipment etc. need be arranged.
The object of the present invention is to provide a kind ofly both to have had good low temperature resistant (196 ℃) performance, and easy to process, safety, is organized storage bag at the medical cell that cost is low.
The objective of the invention is to realize: with ethylene-vinyl acetate copolymer (EVA) or ethylene-propylene acetoacetic ester (EEA) blow molding, again through the synthetic bag of high frequency heat by following manner.Die temperature is 170 ℃, and the avirulence material discharges in the course of processing.The top of bag can be provided with 1 to 2 adapter.
The anti-profound hypothermia of the present invention's storage bag (196 ℃) is functional, stable chemical performance, and no plasticizer is separated out, to the preservation zero damage of tissue and cell etc.; And machine-shaping is convenient, need not special equipment, and no toxic substance discharges, and general plastic molding and processing plant all can process; The cost of product is low in addition, only is the 1/10-1/15 of fluoroplastic storage bag of the same type.The present invention is applicable to unicellular (as sperm, ovum, tumour cell, diploid cell), haemocyte (as red blood cell, blood platelet, leucocyte, bone marrow cell, fetal liver cell), the embryo, the low temperature or the profound hypothermia long preservation of tissue (as skin, blood vessel, nerve, cornea) and organ (as kidney, liver, heart and spleen etc.) etc. are for the needs of clinical transplantation and experiment.
Below with reference to accompanying drawing the present invention is described in further detail, wherein
Fig. 1 is a scheme drawing of the present invention.
Ethylene-vinyl acetate copolymer (EVA) or ethylene-ethyl acrylate copolymer (EEA) are pressed the commonsense method blow molding, again through the synthetic bag 4 shown in Figure 1 of high frequency heat, seam heat seal in bag 4 one sides connects two adapters 3 identical with the bag material, setting is also identical with bag with the bonding barrier film 2(diaphragm material of tube wall in pipe), the mouth of pipe is with nylon or EVA(or EEA) pipe close 1 made clogs.The The performance test results of product is as follows:
1. mechanical characteristics
Pulling strengrth 14.1MPa
Breaking elongation＞500%
2. blood is preserved test
After under 4 ℃ blood being preserved 28 days in bag of the present invention, recording the glycolysis rate is more than 70% for the 92.2%(acceptance value).
3. acute toxicity test in mice
Test results is qualified.
4. fetal liver cell is preserved test under profound hypothermia
Fetal liver cell was preserved 2 months to 2 years down in profound hypothermia (196 ℃), and the result is as shown in table 1:
1. refuse to dye (can not dye)-cell viability
2. CFU-GM-particle CFU-GM (cell cultivation)
3. be due to the error at measurment
5. clinical practice
Shanghai Xinhua hospital has carried out 6 routine marrow autograft operations.Before the transplanting, marrow has been preserved 7-85 days (196 ℃) respectively in bag of the present invention, and the result is satisfactory, has reached the application autologous bone marrow transplantation, prevents myelosuppressive purpose.
Shanghai Huashan Hospital uses the tire liver and 15 examples such as tumour patient after the marrow treatment chemotherapy and aplastic patient of having preserved 2 months to 2 years in storage bag of the present invention (196 ℃); the result all produces a desired effect; have no adverse reaction, safe in utilization, convenient.
Claims (2)
1, a kind of profound hypothermia (196 ℃) biological cell and organize storage bag, it comprises bag 4 and takes over 3 that the material that it is characterized in that said bag and adapter is ethylene-vinyl acetate copolymer (EVA) or ethylene-ethyl acrylate copolymer (EEA).
2, storage bag according to claim 1 is characterized in that the interior setting of pipe and the bonding barrier film 2 of tube wall of said adapter 3, and the mouth of pipe is provided with pipe close 1.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 87106714 CN1014228B (en) | 1987-09-30 | 1987-09-30 | Medical store bag for deep low temp. preserving |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 87106714 CN1014228B (en) | 1987-09-30 | 1987-09-30 | Medical store bag for deep low temp. preserving |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1032318A true CN1032318A (en) | 1989-04-12 |
| CN1014228B CN1014228B (en) | 1991-10-09 |
Family
ID=4815810
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 87106714 Expired CN1014228B (en) | 1987-09-30 | 1987-09-30 | Medical store bag for deep low temp. preserving |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN1014228B (en) |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101861858A (en) * | 2010-05-26 | 2010-10-20 | 金卫医疗科技(上海)有限公司 | Medical profound hypothermia storage bag having function of protecting liquid inlet pipe |
| CN101869100A (en) * | 2010-05-26 | 2010-10-27 | 金卫医疗科技(上海)有限公司 | Low-temperature preservation bag |
| WO2015021806A1 (en) * | 2013-08-14 | 2015-02-19 | 北京瑞健高科生物科技有限公司 | Container and method for processing, sterilizing, and storing biological tissue |
| CN108378021A (en) * | 2018-03-19 | 2018-08-10 | 英普乐孚生物技术(上海)有限公司 | A kind of stored refrigerated system of lymphocyte |
| CN110121648A (en) * | 2016-11-15 | 2019-08-13 | 贝克顿迪金森法国公司 | The evaluation method of the stability of biological products in prefilled syringe |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1067711C (en) * | 1998-09-14 | 2001-06-27 | 薛世峰 | Preparation of fundamental oil of lubricant oil |
-
1987
- 1987-09-30 CN CN 87106714 patent/CN1014228B/en not_active Expired
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101861858A (en) * | 2010-05-26 | 2010-10-20 | 金卫医疗科技(上海)有限公司 | Medical profound hypothermia storage bag having function of protecting liquid inlet pipe |
| CN101869100A (en) * | 2010-05-26 | 2010-10-27 | 金卫医疗科技(上海)有限公司 | Low-temperature preservation bag |
| CN101869100B (en) * | 2010-05-26 | 2014-01-29 | 金卫医疗科技(上海)有限公司 | Low-temperature preservation bag |
| WO2015021806A1 (en) * | 2013-08-14 | 2015-02-19 | 北京瑞健高科生物科技有限公司 | Container and method for processing, sterilizing, and storing biological tissue |
| CN110121648A (en) * | 2016-11-15 | 2019-08-13 | 贝克顿迪金森法国公司 | The evaluation method of the stability of biological products in prefilled syringe |
| CN110121648B (en) * | 2016-11-15 | 2022-07-26 | 贝克顿迪金森法国公司 | Method for evaluating stability of biological product in prefilled syringe |
| CN108378021A (en) * | 2018-03-19 | 2018-08-10 | 英普乐孚生物技术(上海)有限公司 | A kind of stored refrigerated system of lymphocyte |
Also Published As
| Publication number | Publication date |
|---|---|
| CN1014228B (en) | 1991-10-09 |
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