CN1014228B - Medical store bag for deep low temp. preserving - Google Patents
Medical store bag for deep low temp. preservingInfo
- Publication number
- CN1014228B CN1014228B CN 87106714 CN87106714A CN1014228B CN 1014228 B CN1014228 B CN 1014228B CN 87106714 CN87106714 CN 87106714 CN 87106714 A CN87106714 A CN 87106714A CN 1014228 B CN1014228 B CN 1014228B
- Authority
- CN
- China
- Prior art keywords
- present
- tissue
- profound hypothermia
- preservation
- bag
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
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- Agricultural Chemicals And Associated Chemicals (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Medical Preparation Storing Or Oral Administration Devices (AREA)
Abstract
The present invention provides a profound hypothermia (-196 DEG C) biological cell and tissue preservation bag. Ethene-ethyl acetate copolymers or ethene-ethyl acrylate copolymers carry out blow molding forming and carry out high frequency heat seal to form the profound hypothermia (-196 DEG C) biological cell and tissue preservation bag. The present invention has the advantages of good cold resistance performance (-196 DEG C), stable chemical performance, convenient processing and forming, no toxic substance release in the processing process and low product cost (which is only 1/10 to 1/15 of that of fluorine plastic bags in the same type). The present invention is suitable for the low temperature preservation or the profound hypothermia preservation of single cells, blood cells, embryo, tissue, organ, etc. in order to meet clinical transplant requirements and experimental requirements.
Description
The present invention relates to a kind of medical profound hypothermia cell, tissue preservation container.
Usually, the control and the adjusting of nerve and body fluid have just been lost after cell, tissue and organ exsomatize.Along with the prolongation of holding time, metabolism is proceeded, and nutriment such as sugar will be consumed to the greatest extent.In order to suppress cellular metabolism, prolong cell life, help long preservation, it is under profound hypothermia (196 ℃) condition.Need a kind of suitable container of anti-the profound hypothermia, and this container preferably has certain diaphaneity, so that observe for this reason.General commercially available polyvinylchloride bag can only tolerate-80 ℃ low temperature at the most, obviously can not meet above-mentioned requirements.Tend to the profound hypothermia storage bag that adopts fluoroplastic to make abroad, it is the fluoride plastic bag of F-46 that domestic also someone has manufactured experimently the trade mark, though this material physicochemical property is good, but price very expensive (price of 100 milliliters of bags is a 20-30 unit), processed complex, forming temperature height (mouthful mould needs more than 380 ℃), thereby it is higher to the material requirements of equipment, and the intermediate that produces in the high temperature process process is poison extremely, and special draft equipment etc. need be arranged.
The object of the present invention is to provide a kind ofly both to have had good low temperature resistant (0 ℃--196 ℃) performance, and easy to process, safety, the medical cell that cost is low, tissue are preserved container.
The objective of the invention is to realize:, form through high frequency heat seal again with ethylene-vinyl acetate copolymer (EVA) or (EEA) blowing of ethylene-propylene acetoacetic ester or injection mo(u)lding by following manner.Die temperature is 170 ℃, and the avirulence material discharges in the course of processing.The top of container can be provided with 1 to 2 adapter.
The present invention's the anti-profound hypothermia of preservation container (196 ℃) is functional, stable chemical performance, and no plasticizer is separated out, to the preservation zero damage of tissue and cell etc.; And machine-shaping is convenient, need not special equipment, and no toxic substance discharges, and general plastic molding and processing plant all can process; The cost of product is low in addition, only is the 1/10-1/15 of fluoroplastic storage bag of the same type.As the room temperature preservation container, its performance also is better than the polyvinylchloride bag.The present invention is applicable to unicellular (as sperm, ovum, tumour cell, diploid cell), haemocyte (as red blood cell, blood platelet, leucocyte, bone marrow cell, fetal liver cell), blood plasma and goods thereof, biological products, the embryo, the low temperature or the profound hypothermia long preservation of tissue (as skin, blood vessel, nerve, cornea) and organ (as kidney, liver, heart and spleen etc.) are for the needs of clinical transplantation and experiment.
Below with reference to accompanying drawing the present invention is described in further detail, wherein
Fig. 1 is a scheme drawing of the present invention.
Ethylene-vinyl acetate copolymer (EVA) or ethylene-ethyl acrylate copolymer (EEA) are pressed the commonsense method blow molding, the wall 4 that synthesizes container shown in Figure 1 again through high frequency heat, seam heat seal in wall 4 one sides connects two adapters 3 identical with the wall material, setting is also identical with wall with the bonding barrier film 2(diaphragm material of tube wall in pipe), the mouth of pipe is with nylon or EVA(or EEA) pipe close 1 made clogs.In addition, but also injection mo(u)lding of the present invention.The The performance test results of product is as follows:
1. mechanical characteristics
Pulling strengrth 14.1MPa
Breaking elongation＞500%
2. blood is preserved test
After under 4 ℃ blood being preserved 28 days in container of the present invention, recording the glycolysis rate is more than 70% for the 92.2%(acceptance value).
3. acute toxicity test in mice
Test results is qualified.
4. fetal liver cell is preserved test under profound hypothermia
Fetal liver cell was preserved 2 months to 2 years down in profound hypothermia (196 ℃), and the result is as shown in table 1: (table is seen the literary composition back)
5. clinical practice
Shanghai Xinhua hospital has carried out 6 routine marrow autograft operations.Before the transplanting, marrow has been preserved 7-85 days (196 ℃) respectively in bag of the present invention, and the result is satisfactory, has reached the application autologous bone marrow transplantation, prevents myelosuppressive purpose.
Shanghai Huashan Hospital uses the tire liver and 15 examples such as tumour patient after the marrow treatment chemotherapy and aplastic patient of having preserved 2 months to 2 years in preservation container of the present invention (196 ℃); the result all produces a desired effect; have no adverse reaction, safe in utilization, convenient.
Table 1
After preserving before preserving
Preserve example
Time limit counts cell count and refuses to dye
1.CFU-GM
2.Cell count refuses to dye CFU-GM
Only/*/milliliter of milliliter % colony % clone
2 months 18 34452.8 60.2 118.2 31511.1 57.4 193.8
12589.9 19.3 78.3 12455.3 14.7 135.1
4-8 16 32140.6 54.6 139.2 29275 57 116.8
Individual month 17244.3 22.8 95.1 1520.78 18.3 76.8
1 year 9 34016.7 75.9 101.3 30438.9 54.2 98.1
14301.4 13.7 62.4 12771.8 18.4 117.7
2 years 3 30533.3 66 32 25,800 41.7 35
15661.1 19 85 11094.4 9 10.6
* being less than or equal to 50 cells is a colony.
Continuous table 1
The percent recovery
Preserve example
Time limit counts cell count and refuses to dye CFU-GM
% % %
2 18 91.5 95.4 164
3.
Month
4-8 16 91 104.4
③83.9
Individual month
1 year 9 89.5 71.4 96.9
2 years 3 84.5 63.1 109.4
3.
1. refuse to dye (can not dye)-cell viability
2. CFU-GM-particle CFU-GM (cell cultivation)
3. be due to the error at measurment
Claims (2)
1, a kind of profound hypothermia (0 ℃-196 ℃) biological cell and tissue are preserved container, it comprises wall body 4 and takes over 3 that the material that it is characterized in that said bag and adapter is ethene one vinyl acetate co-polymer (EVA) or ethylene-ethyl acrylate copolymer (EEA).
2, preservation container according to claim 1 is characterized in that the interior setting of pipe and the bonding barrier film 2 of tube wall of said adapter 3, and the mouth of pipe is provided with pipe close 1.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 87106714 CN1014228B (en) | 1987-09-30 | 1987-09-30 | Medical store bag for deep low temp. preserving |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 87106714 CN1014228B (en) | 1987-09-30 | 1987-09-30 | Medical store bag for deep low temp. preserving |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1032318A CN1032318A (en) | 1989-04-12 |
| CN1014228B true CN1014228B (en) | 1991-10-09 |
Family
ID=4815810
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 87106714 Expired CN1014228B (en) | 1987-09-30 | 1987-09-30 | Medical store bag for deep low temp. preserving |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN1014228B (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1067711C (en) * | 1998-09-14 | 2001-06-27 | 薛世峰 | Preparation of fundamental oil of lubricant oil |
Families Citing this family (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101869100B (en) * | 2010-05-26 | 2014-01-29 | 金卫医疗科技(上海)有限公司 | Low-temperature preservation bag |
| CN101861858B (en) * | 2010-05-26 | 2014-01-29 | 金卫医疗科技(上海)有限公司 | Medical profound hypothermia storage bag having function of protecting liquid inlet pipe |
| CN103392691B (en) * | 2013-08-14 | 2015-09-23 | 北京瑞健高科生物科技有限公司 | The container of a kind of tissue processing, sterilizing and preservation and method |
| ES2826992T3 (en) * | 2016-11-15 | 2021-05-19 | Becton Dickinson France | Evaluation of the stability of a biological product in pre-filled syringes |
| CN108378021A (en) * | 2018-03-19 | 2018-08-10 | 英普乐孚生物技术(上海)有限公司 | A kind of stored refrigerated system of lymphocyte |
-
1987
- 1987-09-30 CN CN 87106714 patent/CN1014228B/en not_active Expired
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1067711C (en) * | 1998-09-14 | 2001-06-27 | 薛世峰 | Preparation of fundamental oil of lubricant oil |
Also Published As
| Publication number | Publication date |
|---|---|
| CN1032318A (en) | 1989-04-12 |
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