CN103215208A - Haemophilus parasuis culture medium - Google Patents
Haemophilus parasuis culture medium Download PDFInfo
- Publication number
- CN103215208A CN103215208A CN2013101352889A CN201310135288A CN103215208A CN 103215208 A CN103215208 A CN 103215208A CN 2013101352889 A CN2013101352889 A CN 2013101352889A CN 201310135288 A CN201310135288 A CN 201310135288A CN 103215208 A CN103215208 A CN 103215208A
- Authority
- CN
- China
- Prior art keywords
- haemophilus parasuis
- culture solution
- basic culture
- substratum
- sodium
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Landscapes
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention discloses a haemophilus parasuis culture medium. A preparation method of the haemophilus parasuis culture medium comprises the steps of preparing a basic culture solution, treating coenzyme A, and perfecting a culture medium, wherein the basic culture solution comprises peptone, tryptone, sodium chloride, dextrose, yeast extract, glycerin and distilled water, and the basic culture solution, the coenzyme A and fetal bovine serum are uniformly mixed so as to obtain the haemophilus parasuis culture medium. The haemophilus parasuis culture medium provided by the invention can keep haemophilus parasuis, thereby facilitating the transportation of suspicious haemophilus parasuis samples; and the death of haemophilus parasuis is not caused in the process of transportation, thereby increasing the separation probability of the haemophilus parasuis.
Description
Technical field
The invention belongs to agriculture microbial technology field, be specifically related to a kind of haemophilus parasuis substratum.
Background technology
Haemophilus parasuis (Haemophilus parasuis) can cause polyserositis and the sacroiliitis of pig.This bacterium is very tender and lovely, substratum is required harsh relatively, growth relies on Reduced nicotinamide-adenine dinucleotide (NAD or the V factor), do not need the X factor (protoheme and other porphyrin substance), the speed of growth is extremely slow, and vitro culture is preserved easily dead, is cultivating on the blood agar plate more than the 72h, just can grow the big small colonies of needle point, HPS is as easy as rolling off a log to be left in the basket or to be covered by other bacterium.
Haemophilus parasuis is all responsive to freezing, heat, the sample of separation of bacterial, need inoculation medium immediately when gathering as synovial fluid, hydrothorax etc., otherwise separation and Culture often can not be successful, but in real work to collection in worksite immediately inoculation medium be seldom a part, poultry master or basic unit's animal doctor personnel collected specimens often need to refrigerate or deliver to after freezing for some time the laboratory or deliver by the mode of transportation, and it is lower that sample arrives back separation and Culture plating efficiency.Therefore, run into very big difficulty during this bacterium of separation and Culture.
The TSA(tryptose soya agar of the import of report use at present) with the TSB(pancreas peptone soybean broth) as the basic medium that separates haemophilus parasuis, these substratum nutrition are extremely abundant, and HPS speed of growth on these substratum is very fast.If its substratum as transportation haemophilus parasuis suspicious specimen, because may also there be bacterium such as intestinal bacteria, suis in suspicious specimen, these bacterial classifications speed of growth on TSA or TSB is faster more than the haemophilus parasuis growth, just can be intact in short period of time the nutrient consumption of substratum, and the refuse that its growth produces can kill haemophilus parasuis.Therefore, TSA and TSB also are not suitable as the substratum that transports suspicious specimen, also are not suitable as the substratum of preserving haemophilus parasuis.Press for a kind of substratum of suitable transportation haemophilus parasuis suspicious specimen clinically.
Summary of the invention
The invention provides a kind of haemophilus parasuis substratum, be prepared from by following preparation method, its preparation method comprises the perfect of basic culture solution preparation, coenzyme A processing and substratum, and concrete steps are as follows:
1) described basic culture solution preparation
A, with component 14 ~ 16g/L(weight/volume meter of basic culture solution, down together) (count by volume peptone, 4 ~ 6g/L Tryptones, 4 ~ 5g/L sodium-chlor, 2 ~ 3g/L glucose, 4 ~ 6g/L yeast leach liquor and 13 ~ 18 ‰, glycerine down together), surplus distilled water mixes;
B, the mixed solution that the A step is obtained are heated to boiling and constantly stir and dissolve fully until mixed solution;
C, the solution that obtains in the B step is regulated pH value to 7.0 ~ 7.2 by sodium hydroxide;
D, the solution packing that the C step is obtained at 121 ℃ of sterilization 15 ~ 20min down, promptly get basic culture solution after the cooling;
2) described coenzyme A is handled
Get 0.14 ~ 0.16g/L coenzyme A, with 8 ~ 12 ‰ sterilized water dilute filtration degerming;
3) substratum is perfect
Every 100ml basic culture solution adds 1ml coenzyme A diluent and the aseptic calf serum of 5ml with aseptic formality, and mixing installs to the substratum branch in the test tube, every test tube 10 ~ 15ml, and 4 ~ 5 ℃ of preservations are standby.
The component of above-described basic culture solution is that peptone is that 15g/L, Tryptones are that 5g/L, sodium-chlor are that 5g/L, glucose are that 2g/L, yeast leach liquor are that 5g/L and glycerine are 15 ‰.
The component of above-described basic culture solution is that peptone is that 14g/L, Tryptones are that 6g/L, sodium-chlor are that 5g/L, glucose are that 2g/L, yeast leach liquor are that 5g/L and glycerine are 15 ‰.
The component protein peptone that above-described basic culture solution is is that 16g/L, Tryptones are that 4g/L, sodium-chlor are that 4g/L, glucose are that 3g/L, yeast leach liquor are that 4g/L and glycerine are 13 ‰.
The component of above-described basic culture solution is that peptone is that 15g/L, Tryptones are that 5g/L, sodium-chlor are that 4g/L, glucose are that 3g/L, yeast leach liquor are that 6g/L and glycerine are 18 ‰.
The component of above-described basic culture solution is that peptone is that 15g/L, Tryptones are that 5g/L, sodium-chlor are that 5g/L, glucose are that 2g/L, yeast leach liquor are that 6g/L and glycerine are 16 ‰.
The component of above-described basic culture solution is that peptone is that 14g/L, Tryptones are that 5g/L, sodium-chlor are that 4g/L, glucose are that 3g/L, yeast leach liquor are that 6g/L and glycerine are 14 ‰.
The component of above-described basic culture solution is that peptone is that 15g/L, Tryptones are that 5g/L, sodium-chlor are that 4g/L, glucose are that 3g/L, yeast leach liquor are that 5g/L and glycerine are 17 ‰.
Characteristics of the present invention: the nutritive substances such as peptone in the substratum of the present invention just in time satisfy the subsistence level of haemophilus parasuis survival, and its speed of growth is slower; And basic culture solution of the present invention adds glycerine, and glycerine can replenish the required carbon source of bacterial growth on the one hand; Glycerine is a kind of hydroaropic substance on the other hand, work to keep osmotic pressure, keep the integrity effect of bacteria cell wall, guarantee that the haemophilus parasuis survival time is longer, make the activity of keeping bacterium in the transportation haemophilus parasuis sample process, reach and improve the purpose of separating plating efficiency.Progress of the present invention: 1) be convenient to transportation in the aseptic technique mode, haemophilus parasuis suspicious specimen bacterial strain is put into substratum of the present invention, be loaded in the test tube, under the room temperature situation, can transport by the mode of regular bus shipping, the preservation haulage time is 12 ~ 72h.2) improve and the substratum of bacterium probability substratum of the present invention, at room temperature preserve transportation, carry out separation and Culture again after arriving the laboratory, can obviously improve the success ratio of first isolating haemophilus parasuis bacterial strain as transportation haemophilus parasuis suspicious specimen.
Embodiment
In contriver's research to the haemophilus parasuis disease in recent years, improve the flow process of cultivating this bacterium repeatedly, sum up the substratum of transportation haemophilus parasuis suspicious specimen, and substratum is delivered to poultry advocate peace the animal doctor personnel of basic unit on hand, instruct their mode with aseptic technique, sample is put into substratum, mode by the regular bus shipping under the situation of room temperature is transported to the contriver on hand, arrive the laboratory and inoculate the separation haemophilus parasuis again, this method has improved the plating efficiency that separates haemophilus parasuis.
Embodiment 1
Get 15g/L peptone, 5g/L Tryptones, 5g/L sodium-chlor, 2g/L glucose, 5g/L yeast leach liquor and 15 ‰ glycerine, surplus distilled water mixes, be heated to boiling and constantly stir and dissolve fully until mixed solution, solution is regulated pH value to 7.0 ~ 7.2 by sodium hydroxide, the solution that regulates the pH value is carried out packing, at 121 ℃ of 15 ~ 20min that sterilize down, promptly get basic culture solution after the cooling; Get the 0.15g/L coenzyme A, with 10 ‰ sterilized water dissolved dilution filtration sterilizations; Every 100ml basic culture solution adds 1ml coenzyme A diluent and the aseptic calf serum of 5ml with aseptic formality, and mixing installs to the substratum branch in the test tube, every test tube 10 ~ 15ml, and 4 ~ 5 ℃ of preservations get the haemophilus parasuis substratum.
Embodiment 2
Get 14g/L peptone, 6g/L Tryptones, 5g/L sodium-chlor, 2g/L glucose, 5g/L yeast leach liquor and 15 ‰ glycerine, surplus distilled water mixes, be heated to boiling and constantly stir and dissolve fully until mixed solution, solution is regulated pH value to 7.0 ~ 7.2 by sodium hydroxide, the solution that regulates the pH value is carried out packing, at 121 ℃ of 15 ~ 20min that sterilize down, promptly get basic culture solution after the cooling; Get the 0.14g/L coenzyme A, with 8 ‰ sterilized water dissolved dilution filtration sterilizations; Every 100ml basic culture solution adds 1ml coenzyme A diluent and the aseptic calf serum of 5ml with aseptic formality, and mixing installs to the substratum branch in the test tube, every test tube 10 ~ 15ml, and 4 ~ 5 ℃ of preservations get the haemophilus parasuis substratum.
Embodiment 3
Get 16g/L peptone, 4g/L Tryptones, 4g/L sodium-chlor, 3g/L glucose, 4g/L yeast leach liquor and 13 ‰ glycerine, surplus distilled water mixes, be heated to boiling and constantly stir and dissolve fully until mixed solution, solution is regulated pH value to 7.0 ~ 7.2 by sodium hydroxide, the solution that regulates the pH value is carried out packing, at 121 ℃ of 15 ~ 20min that sterilize down, promptly get basic culture solution after the cooling; Get the 0.15g/L coenzyme A, filter out bacterium with 10 ‰ sterilized water dissolved dilutions; Every 100ml basic culture solution adds 1ml coenzyme A diluent and the aseptic calf serum of 5ml with aseptic formality, and mixing installs to the substratum branch in the test tube, every test tube 10 ~ 15ml, and 4 ~ 5 ℃ of preservations get the haemophilus parasuis substratum.
Embodiment 4
Get 15g/L peptone, 5g/L Tryptones, 4g/L sodium-chlor, 3g/L glucose, 6g/L yeast leach liquor and 18 ‰ glycerine, surplus distilled water mixes, be heated to boiling and constantly stir and dissolve fully until mixed solution, solution is regulated pH value to 7.0 ~ 7.2 by sodium hydroxide, the solution that regulates the pH value is carried out packing, at 121 ℃ of 15 ~ 20min that sterilize down, promptly get basic culture solution after the cooling; Get the 0.16g/L coenzyme A, filter out bacterium with 12 ‰ sterilized water dissolved dilutions; Every 100ml basic culture solution adds 1ml coenzyme A diluent and the aseptic calf serum of 5ml with aseptic formality, and mixing installs to the substratum branch in the test tube, every test tube 10 ~ 15ml, and 4 ~ 5 ℃ of preservations get the haemophilus parasuis substratum.
Embodiment 5
Get 15g/L peptone, 5g/L Tryptones, 5g/L sodium-chlor, 2g/L glucose, 6g/L yeast leach liquor and 16 ‰ glycerine, surplus distilled water mixes, be heated to boiling and constantly stir and dissolve fully until mixed solution, solution is regulated pH value to 7.0 ~ 7.2 by sodium hydroxide, the solution that regulates the pH value is carried out packing, at 121 ℃ of 15 ~ 20min that sterilize down, promptly get basic culture solution after the cooling; Get the 0.15g/L coenzyme A, with 10 ‰ sterilized water dissolved dilution filtration sterilizations; Every 100ml basic culture solution adds 1ml coenzyme A diluent and the aseptic calf serum of 5ml with aseptic formality, and mixing installs to the substratum branch in the test tube, every test tube 10 ~ 15ml, and 4 ~ 5 ℃ of preservations get the haemophilus parasuis substratum.
Embodiment 6
Get 14g/L peptone, 5g/L Tryptones, 4g/L sodium-chlor, 3g/L glucose, 6g/L yeast leach liquor and 14 ‰ glycerine, surplus distilled water mixes, be heated to boiling and constantly stir and dissolve fully until mixed solution, solution is regulated pH value to 7.0 ~ 7.2 by sodium hydroxide, the solution that regulates the pH value is carried out packing, at 121 ℃ of 15 ~ 20min that sterilize down, promptly get basic culture solution after the cooling; Get the 0.15g/L coenzyme A, with 10 ‰ sterilized water dissolved dilution filtration sterilizations; Every 100ml basic culture solution adds 1ml coenzyme A diluent and the aseptic calf serum of 5ml with aseptic formality, and mixing installs to the substratum branch in the test tube, every test tube 10 ~ 15ml, and 4 ~ 5 ℃ of preservations get the haemophilus parasuis substratum.
Embodiment 7
Get 15g/L peptone, 5g/L Tryptones, 4g/L sodium-chlor, 3g/L glucose, 5g/L yeast leach liquor and 17 ‰ glycerine, surplus distilled water mixes, be heated to boiling and constantly stir and dissolve fully until mixed solution, solution is regulated pH value to 7.0 ~ 7.2 by sodium hydroxide, the solution that regulates the pH value is carried out packing, at 121 ℃ of 15 ~ 20min that sterilize down, promptly get basic culture solution after the cooling; Get the 0.15g/L coenzyme A, with 10 ‰ sterilized water dissolved dilution filtration sterilizations; Every 100ml basic culture solution adds 1ml coenzyme A diluent and the aseptic calf serum of 5ml with aseptic formality, and mixing installs to the substratum branch in the test tube, every test tube 10 ~ 15ml, and 4 ~ 5 ℃ of preservations get the haemophilus parasuis substratum.
Embodiment 8
When the substratum transportation of embodiment 1 preparation is separated the haemophilus parasuis sample, tissue samples such as the blood of aseptic collection, synovial fluid, hydrothorax, lung are put into substratum, be loaded in 10 ~ 15ml test tube, building cap seal installs, vertically be placed on (temperature adds an amount of ice bag when surpassing 40 ℃, the prevention temperature is too high) good seal transportation in the suitable foam case.Can not stand upside down in the transportation, the mode by the regular bus shipping under the situation of room temperature is transported to the contriver on hand, behind the arrival laboratory, culture medium inoculated is separated on the substratum that separates haemophilus parasuis.
Embodiment 9
Table 1: use substratum preservation transportation tissue sample of the present invention and freezing preservation transportation sample to go out the bacterium effect relatively
The result shows: 1, the sample separation plating efficiency of two kinds of preserving types does not have significant difference below 12h the shelf time; Shelf time, substratum of the present invention was isolated haemophilus parasuis between 12 ~ 72h, and freezing preservation does not have haemophilus parasuis and occurs; Two kinds of methods did not all have haemophilus parasuis and occurred more than 72h shelf time.2, substratum of the present invention is at room temperature preserved transportation and is arrived the laboratory, still separablely goes out haemophilus parasuis, and freezing preservation need be lowered the temperature, and isolates the bacterium situation and obviously be inferior to substratum of the present invention.
Claims (8)
1. haemophilus parasuis substratum is characterized in that: be prepared from by following preparation method, its preparation method comprises that basic culture solution preparation, coenzyme A are handled and substratum perfect, and concrete steps are as follows:
1) described basic culture solution preparation
A, with component 14 ~ 16g/L(weight/volume meter of basic culture solution, down together) (count by volume peptone, 4 ~ 6g/L Tryptones, 4 ~ 5g/L sodium-chlor, 2 ~ 3g/L glucose, 4 ~ 6g/L yeast leach liquor and 13 ~ 18 ‰, glycerine down together), surplus distilled water mixes;
B, the mixed solution that the A step is obtained are heated to boiling and constantly stir and dissolve fully until mixed solution;
C, the solution that obtains in the B step is regulated pH value to 7.0 ~ 7.2 by sodium hydroxide;
D, the solution packing that the C step is obtained at 121 ℃ of sterilization 15 ~ 20min down, promptly get basic culture solution after the cooling;
2) described coenzyme A is handled
Get 0.14 ~ 0.16g/L coenzyme A, with 8 ~ 12 ‰ sterilized water dilute filtration degerming;
3) substratum is perfect
Every 100ml basic culture solution adds 1ml coenzyme A diluent and the aseptic calf serum of 5ml with aseptic formality, and mixing installs to the substratum branch in the test tube, every test tube 10 ~ 15ml, and 4 ~ 5 ℃ of preservations are standby.
2. haemophilus parasuis substratum according to claim 1 is characterized in that: the component of described basic culture solution is that peptone is that 15g/L, Tryptones are that 5g/L, sodium-chlor are that 5g/L, glucose are that 2g/L, yeast leach liquor are that 5g/L and glycerine are 15 ‰.
3. haemophilus parasuis substratum according to claim 1 is characterized in that: the component of described basic culture solution is that peptone is that 14g/L, Tryptones are that 6g/L, sodium-chlor are that 5g/L, glucose are that 2g/L, yeast leach liquor are that 5g/L and glycerine are 15 ‰.
4. haemophilus parasuis substratum according to claim 1 is characterized in that: the component of described basic culture solution is that peptone is that 16g/L, Tryptones are that 4g/L, sodium-chlor are that 4g/L, glucose are that 3g/L, yeast leach liquor are that 4g/L and glycerine are 13 ‰.
5. haemophilus parasuis substratum according to claim 1 is characterized in that: the component of described basic culture solution is that peptone is that 15g/L, Tryptones are that 5g/L, sodium-chlor are that 4g/L, glucose are that 3g/L, yeast leach liquor are that 6g/L and glycerine are 18 ‰.
6. haemophilus parasuis substratum according to claim 1 is characterized in that: the component of described basic culture solution is that peptone is that 15g/L, Tryptones are that 5g/L, sodium-chlor are that 5g/L, glucose are that 2g/L, yeast leach liquor are that 6g/L and glycerine are 16 ‰.
7. haemophilus parasuis substratum according to claim 1 is characterized in that: the component of described basic culture solution is that peptone is that 14g/L, Tryptones are that 5g/L, sodium-chlor are that 4g/L, glucose are that 3g/L, yeast leach liquor are that 6g/L and glycerine are 14 ‰.
8. haemophilus parasuis substratum according to claim 1 is characterized in that: the component of described basic culture solution is that peptone is that 15g/L, Tryptones are that 5g/L, sodium-chlor are that 4g/L, glucose are that 3g/L, yeast leach liquor are that 5g/L and glycerine are 17 ‰.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310135288.9A CN103215208B (en) | 2013-04-18 | 2013-04-18 | Haemophilus parasuis culture medium |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310135288.9A CN103215208B (en) | 2013-04-18 | 2013-04-18 | Haemophilus parasuis culture medium |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN103215208A true CN103215208A (en) | 2013-07-24 |
| CN103215208B CN103215208B (en) | 2015-04-08 |
Family
ID=48813372
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201310135288.9A Active CN103215208B (en) | 2013-04-18 | 2013-04-18 | Haemophilus parasuis culture medium |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN103215208B (en) |
Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103667160A (en) * | 2013-12-30 | 2014-03-26 | 山东滨州博莱威生物技术有限公司 | High-density fermentation culture medium for haemophilus parasuis |
| CN105018347A (en) * | 2015-07-29 | 2015-11-04 | 傅石明 | Method for preserving hemophilus strain |
| CN106282075A (en) * | 2016-11-02 | 2017-01-04 | 青岛易邦生物工程有限公司 | A kind of fluid medium for cultivating haemophilus parasuis |
| CN106434480A (en) * | 2016-11-02 | 2017-02-22 | 青岛易邦生物工程有限公司 | High-density fermentation and culture method of Haemophilus parasuis |
| CN107177531A (en) * | 2017-06-15 | 2017-09-19 | 广东海大畜牧兽医研究院有限公司 | A kind of growth accelerator for improving haemophilus parasuis in vitro culture |
| CN108977392A (en) * | 2018-08-14 | 2018-12-11 | 沈阳农业大学 | A kind of haemophilus parasuis proliferated culture medium and preparation method thereof |
| CN112625969A (en) * | 2020-12-31 | 2021-04-09 | 天津瑞普生物技术股份有限公司 | Culture medium for clinical separation of haemophilus parasuis and separation method |
| CN112831428A (en) * | 2019-11-25 | 2021-05-25 | 北京信得威特科技有限公司 | Haemophilus parasuis culture medium |
| CN114149951A (en) * | 2021-12-30 | 2022-03-08 | 国药集团动物保健股份有限公司 | Haemophilus parasuis culture medium and preparation method thereof |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| RU2268933C1 (en) * | 2004-04-21 | 2006-01-27 | Федеральное государственное учреждение "Федеральный центр охраны здоровья животных" (ФГУ ВНИИЗЖ) | STRAIN Haemophilus parasuis SK-1 AS PATHOGEN OF SWINE HEMOPHILEOUS POLYSEROSITIS FOR PREPARING DIAGNOSTIC AND VACCINE PREPARATIONS |
| CN101955916A (en) * | 2010-07-06 | 2011-01-26 | 江苏省农业科学院 | Wide-host spectrum listeria phage and preparation method and application thereof |
-
2013
- 2013-04-18 CN CN201310135288.9A patent/CN103215208B/en active Active
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| RU2268933C1 (en) * | 2004-04-21 | 2006-01-27 | Федеральное государственное учреждение "Федеральный центр охраны здоровья животных" (ФГУ ВНИИЗЖ) | STRAIN Haemophilus parasuis SK-1 AS PATHOGEN OF SWINE HEMOPHILEOUS POLYSEROSITIS FOR PREPARING DIAGNOSTIC AND VACCINE PREPARATIONS |
| CN101955916A (en) * | 2010-07-06 | 2011-01-26 | 江苏省农业科学院 | Wide-host spectrum listeria phage and preparation method and application thereof |
Non-Patent Citations (2)
| Title |
|---|
| T.O’REILLY: "Tryptone-Yeast Extract Broth as a Culture Medium for Haemophilus pleuropneumoniae and Haemophilus parasuis to be used as Challenge Inocula", 《CAN J VET RES》 * |
| 宋德平: "副猪嗜血杆菌江西株的分离鉴定及生物学特性研究", 《江西农业大学学报》 * |
Cited By (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103667160A (en) * | 2013-12-30 | 2014-03-26 | 山东滨州博莱威生物技术有限公司 | High-density fermentation culture medium for haemophilus parasuis |
| CN105018347A (en) * | 2015-07-29 | 2015-11-04 | 傅石明 | Method for preserving hemophilus strain |
| CN106282075A (en) * | 2016-11-02 | 2017-01-04 | 青岛易邦生物工程有限公司 | A kind of fluid medium for cultivating haemophilus parasuis |
| CN106434480A (en) * | 2016-11-02 | 2017-02-22 | 青岛易邦生物工程有限公司 | High-density fermentation and culture method of Haemophilus parasuis |
| CN107177531A (en) * | 2017-06-15 | 2017-09-19 | 广东海大畜牧兽医研究院有限公司 | A kind of growth accelerator for improving haemophilus parasuis in vitro culture |
| CN107177531B (en) * | 2017-06-15 | 2018-07-06 | 广东海大畜牧兽医研究院有限公司 | A kind of growth accelerator for improving haemophilus parasuis in vitro culture |
| CN108977392A (en) * | 2018-08-14 | 2018-12-11 | 沈阳农业大学 | A kind of haemophilus parasuis proliferated culture medium and preparation method thereof |
| CN108977392B (en) * | 2018-08-14 | 2021-08-06 | 沈阳农业大学 | Haemophilus parasuis proliferation medium and preparation method thereof |
| CN112831428A (en) * | 2019-11-25 | 2021-05-25 | 北京信得威特科技有限公司 | Haemophilus parasuis culture medium |
| CN112625969A (en) * | 2020-12-31 | 2021-04-09 | 天津瑞普生物技术股份有限公司 | Culture medium for clinical separation of haemophilus parasuis and separation method |
| CN112625969B (en) * | 2020-12-31 | 2022-04-12 | 天津瑞普生物技术股份有限公司 | Culture medium for clinical separation of haemophilus parasuis and separation method |
| CN114149951A (en) * | 2021-12-30 | 2022-03-08 | 国药集团动物保健股份有限公司 | Haemophilus parasuis culture medium and preparation method thereof |
Also Published As
| Publication number | Publication date |
|---|---|
| CN103215208B (en) | 2015-04-08 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN103215208B (en) | Haemophilus parasuis culture medium | |
| CN102311919B (en) | Microalgae species preserving method | |
| Joblin | Methanogenic archaea | |
| CN103060220A (en) | Low-serum culture medium for efficiently culturing mycoplasma hyopneumoniae and preparation method thereof | |
| CN105211052B (en) | Frozen stock solution of cultured NKT cells and preparation method thereof | |
| KR20150139142A (en) | Feed additive composition for reducing methane gas produced by ruminant animals | |
| CN105039202B (en) | A kind of salmonella, Listeria monocytogenes and the compound selective medium for increasing bacterium of vibrio parahaemolytious and preparation method thereof | |
| CN103710290A (en) | Probiotics lactobacillus rhamnosus hsryfm1301 originated in Guangxi Bama longevity village and application of probiotics lactobacillus rhamnosus hsryfm1301 | |
| CN108018230A (en) | A kind of natural less-virulent strain of serum 7-type haemophilus parasuis and its application | |
| CN104894030A (en) | Lactococcus lactis subsp.lactis and application of lactococcus lactis subsp.lactis in low-temperature silage | |
| Pangloli et al. | Evaluation of methods for recovery of Salmonella from dairy cattle, poultry, and swine farms | |
| CN109400392A (en) | A kind of astaxanthin fertilizer and preparation method thereof containing microorganism | |
| CN111484958B (en) | Lactobacillus plantarum with ETEC (ethylene-tetra-ethyl-carbonate) inhibition effect, fermentation product and application thereof | |
| CN102533561B (en) | Protecting agent for freezing preservation of edible fungus strain and use method | |
| CN107232184B (en) | Diluent for preserving semen of black pig in Guanzhong at normal temperature | |
| CN106434479A (en) | High-density culture method of fermentation tank of 500 L for model-5 haemophilus parasuis | |
| CN102286411A (en) | Lactobacillus plantarum and application thereof in fermenting cabbage wrapper leaf | |
| CN104212745A (en) | Chicken microecological preparation and preparation method thereof | |
| Amin | Screening of Cellulose-Degrading Bacteria Associated with Gastrointestinal Tract of Hybrid Abalone as Probiotic Candidates | |
| CN110904007B (en) | Animal clostridium novyi exotoxin, preparation method thereof, toxigenic culture medium and application | |
| CN104740627B (en) | A kind of large-scale method for producing of pseudo- mad dog attenuated live vaccines for animals | |
| CN108130292B (en) | Streptomyces marinus S063 and application of anticomplement activity thereof | |
| CN112458030A (en) | Preparation method of clostridium butyricum preparation special for ruminants | |
| CN110583630A (en) | Donkey semen diluent and preparation method thereof | |
| CN104911131A (en) | Enterococcus mundtii and application thereof in low-temperature ensiling |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20221028 Address after: 530000 Site 103, the first floor, Building A-1 #, Biological Industry Incubation Park, No. 18, Xinji Road, Nanning City, Guangxi Zhuang Autonomous Region Patentee after: Guangxi Yuemu Biotechnology Co.,Ltd. Address before: 530001 No.51 Youai North Road, XiXiangTang District, Nanning City, Guangxi Zhuang Autonomous Region Patentee before: GUANGXI VETERINARY Research Institute |
|
| TR01 | Transfer of patent right |