CN103211677B - Retina extractor - Google Patents
Retina extractor Download PDFInfo
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- CN103211677B CN103211677B CN201310162184.7A CN201310162184A CN103211677B CN 103211677 B CN103211677 B CN 103211677B CN 201310162184 A CN201310162184 A CN 201310162184A CN 103211677 B CN103211677 B CN 103211677B
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Abstract
The invention relates to a retina extractor used for an operation in the experimental ophthalmology. The retina extractor comprises a handle, a retina extracting spoon is arranged at one end of the handle, and a blade is arranged at the other end of the handle. A certain inclination angle is formed between the plane where the retina extracting spoon is located and an axis of the handle. A blade point is arranged at the front end of the blade. Insertion grooves are formed in the two ends of the handle respectively, and matched with the retina extracting spoon and the blade. Devices are arranged in the insertion grooves and used for fixing the retina extracting spoon and the blade. The handle, the extracting spoon and the blade are designed integrally, the design is exquisite and reasonable and meets the body mechanics principle, acquisition time of the retina can be greatly shortened, a using method and steps are simple and convenient to implement, and good conditions are created for the survival rate of nerve cells of the retina. The retina extractor is suitable for being used by researchers and medical workers at different levels in the field and worthy of being popularized.
Description
Technical field
The present invention relates to a kind of surgery medical apparatus and instruments, particularly relate to the retina extractor of performing the operation in a kind of experimental eye science.
Background technology
Retina is the pith of eye structure, is the layer of transparent thin film being positioned at wall of eyeball internal layer, is made up of layer of retina,pigment epithelium and cerebral stratum of retina.The information that the mankind obtain from the external world, more than 80% comes from visual system.Statistics shows, and about there are 1.6 hundred million visual disorder patients in the whole world, 4,000 ten thousand legal blind persons.China is the country that whole world blind person is maximum, about has 7,000,000, and China about has 450,000 people blind every year, the injury caused because of blinding oculopathy and cannot estimating to the heavy burden that society causes.Retinal diseases is the most common cause (more than 50%) of developed country's blinding, and China, every year because of the patient about 200,000 (accounting for 45%) that this type of disease is blind, is the serious blinding oculopathy that there is no effective treatment means at present in the world.Therefore, the research of Efforts To Develop retinal development and gene regulation rule, for the pathogenesis of retinal diseases is extremely urgent with the research finding effective remedy measures.
At present, in domestic and international visual science research field, the amphiblestroid common method of external acquisition is that these two kinds of methods are all consuming time longer, generally need 15-25 minute with enzymic digestion and microdissection separation method.Facts have proved, after retina leaves live body 15-25 minute, the mortality rate of retinal neuronal cell can reach 40-60%, this will produce considerable influence to the retina basic and clinic studies success next carried out, very likely because retina isolated time is long, cause the serious consequence that research is failed.Therefore, be badly in need of improving existing retina and take out technology, retina can be taken out fast and efficiently, improve the survival rate of retinal neuronal cell, to obtain real result of study, for the basic and clinic studies of anti-blind blinding provides technical support.
Summary of the invention
The technical problem to be solved in the present invention: overcome retina in prior art and to draw materials the problem that the time is long, retinal neuronal cell survival rate is low, a kind of retina extractor that can obtain retina fast, improve retinal neuronal cell survival rate is provided.
Technical scheme of the present invention:
A kind of retina extractor, comprises handle, and described handle one end is provided with retina and takes out spoon, and the other end is provided with blade, and described retina takes out spoon place plane and handle axis is certain inclination angle, and the front end of described blade is provided with point of a knife.
The angle of described point of a knife is 20-30 degree, and described inclination angle is 15-30 degree.
Described retina take out spoon with between handle and between blade with handle for being flexibly connected.
Described retina takes out spoon and takes organic plastics to make, and the spoon head cross section that retina takes out spoon is circular, and diameter is 2.5-20mm.
Described handle adopts organic plastics or metal material to make, and handle cross-section is circular, and its diameter is 6-8mm, and handle is long is 80-100mm.
Described retina takes out spoon and blade is disposable product.
Described handle two ends are respectively equipped with the insertion groove taking out spoon and blade with retina and match, and are provided with the device that fixing retina takes out spoon and blade in insertion groove.
the present invention has following advantages:
(1) retina extractor of the present invention, adopting one end of handle to be the retina taking-up spoon other end is the global design of blade, retina takes out spoon place plane and handle place straight line is certain inclination angle, different and the difference to some extent of this inclination angle kind of originating according to retina, the inclination angle that general meiofauna uses is 15-20 degree, and the inclination angle that larger animal or people's eyeball use is about 30 degree.This deft design is reasonable, meets body mechanics's principle, greatly can shorten the amphiblestroid acquisition time, make retina take-off time by the 15-25 minute of conventional method, about shortening to for 5 seconds; Removing method and step are very easy, largely avoided the phenomena of mortality of retinal neuronal cell in retina taking-up process, for the survival rate improving retinal neuronal cell creates good condition.
(2) retina of the present invention takes out the spoon head cross-sectional diameter of spoon is 2.5-20mm, both be applicable to the less animal of eyeball and used (eyeball of mouse diameter is about 4mm), also be applicable to the larger animal or human of eyeball and use (Mini-musk swine eyeball diameter about 20 mm, human eye bulb diameter is about 22mm), the scope of application is wider.
(3) retina of the present invention takes out spoon with all adopting between handle, between blade and handle and is flexibly connected, and takes out spoon and blade can adopt disposable product, can avoid reusing, effectively prevent the mutual pollution between species or between cell.
(4) the present invention is easy to use, and method is easy to grasp, and is suitable for this area research worker at different levels and medical personnel's use, highly promotes.
(5) application of the present invention, is the research of amphiblestroid pathogenesis, retinal development and mechanisms of gene regulation, creates favourable technical support.
Accompanying drawing explanation
Fig. 1, retina extractor structural representation of the present invention;
The decomposition texture schematic diagram of Fig. 2, Fig. 1;
Fig. 3, perspective view of the present invention;
In figure: 1 is retina taking-up spoon, and 2 is handle, and 3 is blade.
Detailed description of the invention
embodiment 1retina extractor, see Fig. 1-3, comprises handle, and handle one end is provided with retina and takes out spoon, and the other end is provided with blade; The wherein long 80mm of handle, its cross section is circular, and diameter is about 6mm, and namely handle is cylindrical-shaped structure; Retina takes out the spoon head cross section of spoon for circle, and diameter is 2.5-10 mm, and the angle taking out spoon place plane and handle place axis is 15 degree; Blade is provided with sharp point of a knife, and the angle at point of a knife place is 30 degree.
Handle two ends are provided with insertion groove, and handle takes out spoon with retina, all adopts inserted mode be connected and fix between handle and blade.Handle adopts rustless steel or organic plastics to make.
the using method of retina extractor:
First, fully rinse with the phosphate buffer (pH7.4) of pre-cooling with containing antibiotic normal saline the eyeball deriving from laboratory animal; Eyeball is placed on eyeball fixer; Wipe out the affiliated group of eyeball surface and courageous and upright attachment, retain four rectus, then four rectus are fixed on eyeball fixer, cut all-round wall of eyeball with the blade of retina extractor along aequator oculi, remove anterior chamber of eye; Take out spoon crystalline lens and vitreous body with retina to take out, then take out complete retina with taking out spoon along subretinal space, put into by retina prior in pre-cooling eppendorf pipe on ice, whole experimentation can complete within 5 seconds.The experimentation that next step is relevant can be carried out after obtaining retina.
embodiment 2substantially identical with embodiment 1, difference is:
The long 90mm of handle of retina extractor, handle cross-section diameter is 7mm, and material adopts organic plastics; Retina spoon head diameter is 11-16mm, and the angle that retina takes out spoon place plane and handle axis is 20 degree,
Handle and retina take out spoon, adopt existing spring gim peg or spring leaf to fix between handle and blade.The angle at point of a knife place is 25 degree, and retina takes out spoon and blade all adopts disposable material to make.
embodiment 3substantially identical with embodiment 1, difference is:
The long 100mm of handle of retina extractor, handle cross-section diameter is 8mm; Spoon head diameter 17-20mm, the angle taking out spoon place plane and handle axis is 30 degree, and point of a knife place angle is 20 degree.Retina takes out spoon and adopts organic plastics to make.
Claims (2)
1. a retina extractor, comprises handle, it is characterized in that: described handle one end is provided with retina and takes out spoon, and the other end is provided with blade, and described retina takes out spoon place plane and handle place axis is certain inclination angle, and the front end of described blade is provided with point of a knife; The angle of described point of a knife is 20-30 degree, and described inclination angle is 15-30 degree; Described retina takes out spoon and takes organic plastics to make, and its spoon head cross section is circular, and diameter is 2.5-20mm.
2. according to the retina extractor described in claim 1, it is characterized in that: described retina takes out spoon with being between handle, between blade with handle and is flexibly connected.
3. according to the retina extractor described in claim 1, it is characterized in that: described handle adopts organic plastics or metal material to make, and handle cross-section is diameter 6-8mm, handle is long is 80-100mm.
4. according to the retina extractor described in claim 1, it is characterized in that: described retina takes out spoon and blade is disposable product.
5. the retina extractor according to any one of claim 1-4, is characterized in that: described handle two ends are respectively equipped with the insertion groove taking out spoon and blade with retina and match, and are provided with the parts that fixing retina takes out spoon or blade in insertion groove.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310162184.7A CN103211677B (en) | 2013-05-06 | 2013-05-06 | Retina extractor |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310162184.7A CN103211677B (en) | 2013-05-06 | 2013-05-06 | Retina extractor |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN103211677A CN103211677A (en) | 2013-07-24 |
| CN103211677B true CN103211677B (en) | 2015-03-18 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201310162184.7A Active CN103211677B (en) | 2013-05-06 | 2013-05-06 | Retina extractor |
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Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103234774B (en) * | 2013-05-06 | 2015-06-03 | 郑州大学第一附属医院 | Method for in vitro separation and preparation of retina nerve cell layer |
| CN111925988B (en) * | 2020-08-29 | 2022-09-23 | 郑州大学 | Method for preparing single cell suspension based on retina tissue/organoid-retina tissue |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN2071060U (en) * | 1990-05-31 | 1991-02-13 | 孙承禄 | Eye muscle choroid insertion operation knife |
| CN103006374A (en) * | 2012-12-27 | 2013-04-03 | 尹澜 | Corneal foreign body draw-out spoon |
| CN203195839U (en) * | 2013-05-06 | 2013-09-18 | 郑州大学第一附属医院 | Retina taking-out instrument |
-
2013
- 2013-05-06 CN CN201310162184.7A patent/CN103211677B/en active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN2071060U (en) * | 1990-05-31 | 1991-02-13 | 孙承禄 | Eye muscle choroid insertion operation knife |
| CN103006374A (en) * | 2012-12-27 | 2013-04-03 | 尹澜 | Corneal foreign body draw-out spoon |
| CN203195839U (en) * | 2013-05-06 | 2013-09-18 | 郑州大学第一附属医院 | Retina taking-out instrument |
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| Publication number | Publication date |
|---|---|
| CN103211677A (en) | 2013-07-24 |
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